An industrial biological enzyme purification device with a cleaning function

Abstract

The present invention pertains to the technical field of biological enzyme preparation, and is mainly an industrial biological enzyme purification device with a cleaning function. It includes a base, the base is fixedly connected with a housing, the outside of the housing is fixedly connected with a control terminal, the top of the housing is fixedly connected with a first motor electrically connected to the control terminal through a connecting member, the output shaft of the first motor is fixedly connected with a first gear, the top of the housing is rotatably connected with a rotating shaft, the bottom and the top inside the housing are respectively rotatably connected with a chassis and a top plate, a first microfiltration membrane is fixedly connected between the chassis and the top plate, a first channel is arranged inside the rotating shaft, and the rotating shaft is fixedly connected with an airbag. By inflating the airbag, the sponge is closely attached to the first microfiltration membrane, and then the impurities attached to the inner side of the first microfiltration membrane are impacted by bubbles, so that the device can clean the first microfiltration membrane without stopping the machine, saving a large amount of time and greatly improving the purification rate of biological enzymes.

Description

Technical Field

[0001] The present invention relates to the technical field of biological enzyme preparation, and particularly relates to an industrial biological enzyme purification device with a cleaning function. Background Art

[0002] Biological enzymes are biological macromolecules that catalyze specific chemical reactions. The vast majority are proteins, and very few are RNA molecules with catalytic activity. When preparing biological enzymes, it is necessary to purify the biological enzymes in the culture solution in order to obtain biological enzymes with higher purity. The microfiltration membrane technology is a means of separating and filtering suspended particles in liquids or gases using a thin film with a certain pore size. It can effectively intercept suspended solids, bacteria, microparticles, some viruses, and colloids in water, while allowing water molecules to pass through, and thus is widely used in the purification of biological enzymes. When microfiltering and purifying biological enzymes, impurities in the culture solution will block the pore size of the microfiltration membrane, resulting in a decrease in flow rate. Most of the existing cleaning methods require shutting down the machine to remove the microfiltration membrane for cleaning, and then reinstalling the microfiltration membrane. This cleaning method is not only time-consuming and laborious, but also greatly reduces the purification rate of biological enzymes. Summary of the Invention

[0003] In order to overcome the disadvantages mentioned in the above background art, the present invention provides an industrial biological enzyme purification device with a cleaning function.

[0004] Technical Solution: An industrial biological enzyme purification device with a cleaning function includes a base. The base is fixedly connected with a housing. The outside of the housing is fixedly connected with a control terminal. The top of the housing is fixedly connected with a first motor electrically connected to the control terminal through a connecting member. The output shaft of the first motor is fixedly connected with a first gear. A rotating shaft is rotatably connected to the top of the housing. The rotating shaft is fixedly connected with a second gear meshing with the first gear outside the housing. The bottom and top inside the housing are respectively rotatably connected with a chassis and a top plate. A first microfiltration membrane is fixedly connected between the chassis and the top plate. The chassis, the top plate, and the first microfiltration membrane divide the housing into an inner cavity and an outer cavity. A first channel is provided inside the rotating shaft. The rotating shaft is fixedly connected with an airbag. The first channel inside the rotating shaft is communicated with the airbag. A sponge fixedly connected to the rotating shaft is provided outside the airbag. The rotating shaft and the airbag are jointly fixedly connected with flexible tubes arranged in a linear array and mirror-symmetrically distributed. The flexible tubes are communicated with the first channel. An air valve is provided at the top of the housing. A liquid inlet pipe communicated with the inner cavity and a liquid outlet pipe communicated with the outer cavity are respectively fixedly connected to the bottom and top of the housing. A blocking assembly for blocking the airbag is provided on the rotating shaft.

[0005] As an improvement of the above solution, the plugging assembly includes a first push rod electrically connected to the control terminal. The first push rod is fixedly connected to the top of the housing through a connecting member. The telescopic end of the first push rod is fixedly connected to a first sliding shaft. The first sliding shaft is fixedly connected to a linear array of plugging rings. The plugging rings in the linear array are all slidably connected to the rotating shaft. The plugging rings in the linear array are in plugging cooperation with the airbag. The plugging rings in the linear array are respectively in plugging cooperation with the flexible tubes distributed in adjacent mirror images. A rotating assembly for rotating the first microfiltration membrane is arranged inside the housing.

[0006] As an improvement of the above solution, the rotating assembly includes a second motor electrically connected to the control terminal. The second motor is fixedly connected to the top of the housing through a connecting member. The output shaft of the second motor is fixedly connected to a rotating shaft. The rotating shaft is fixedly connected to a linear array of third gears. Tooth teeth in a circular array are fixedly connected to the exteriors of the chassis and the top plate. The chassis and the top plate are respectively in transmission connection with the adjacent third gears through the tooth teeth in the circular array.

[0007] As an improvement of the above solution, it further includes a backwashing assembly for backwashing the first microfiltration membrane. The backwashing assembly is arranged inside the housing. The backwashing assembly includes a linear array of backwashing cylinders. The backwashing cylinders in the linear array are all fixedly connected to the inside of the housing through connecting members. A first piston shaft is slidably connected inside the backwashing cylinder. A first elastic element is arranged between the first piston shaft and the adjacent backwashing cylinder. A pushing assembly for pushing the first piston shaft is arranged on the housing.

[0008] As an improvement of the above solution, the pushing assembly includes a second push rod electrically connected to the control terminal. The second push rod is fixedly connected to the top of the housing through a connecting member. The telescopic end of the second push rod is fixedly connected to a second sliding shaft. The second sliding shaft is slidably connected to the housing. The second sliding shaft is fixedly connected to a linear array of extrusion blocks. Trigger rods are fixedly connected to the first piston shafts in the linear array. The extrusion blocks in the linear array are respectively in extrusion cooperation with the adjacent trigger rods.

[0009] As an improvement of the above solution, the extrusion block is a right-angled triangular plate, and the hypotenuse of the extrusion block faces the adjacent backwashing cylinder.

[0010] As an improvement to the above solution, it further includes a docking component for docking the first microfiltration membrane with the backwashing cylinder. The docking component is arranged inside the first microfiltration membrane. The docking component includes a third push rod electrically connected to the control terminal. The third push rod is fixedly connected to the top of the housing through a connecting piece. The housing is fixedly connected with a docking pipe inside it. The telescopic end of the third push rod is fixedly connected with a piston disc slidably connected to the docking pipe. The docking pipe is slidably connected with a linear array of second piston shafts. The second piston shafts are fixedly connected with flexible rings. A second elastic element is arranged between each of the linear array of second piston shafts and the docking pipe.

[0011] As an improvement to the above solution, it further includes a collection component for collecting impurities. The collection component is arranged on the housing. The collection component includes a fourth push rod electrically connected to the control terminal. The fourth push rod is fixedly connected to the top of the housing through a connecting piece. The telescopic end of the fourth push rod is fixedly connected with a third sliding shaft. The third sliding shaft is in contact and cooperation with the chassis. The top of the housing is detachably connected with a plugging disc. The third sliding shaft is slidably connected with the plugging disc. The lower part of the third sliding shaft is fixedly connected with a fixed disc. A second microfiltration membrane is fixedly connected between the fixed disc and the third sliding shaft. The third sliding shaft, the fixed disc and the second microfiltration membrane cooperate to form a sealed cavity. The fixed disc is fixedly connected with an annular array of feed holes. The annular array of feed holes communicates with the sealed cavity.

[0012] As an improvement to the above solution, the feed hole is frustum-shaped, and the end with a smaller diameter of the feed hole is located inside the sealed cavity formed by the cooperation of the third sliding shaft, the fixed disc and the second microfiltration membrane.

[0013] As an improvement to the above solution, a second channel is arranged inside the third sliding shaft. The bottom of the third sliding shaft is provided with an annular array of air outlet holes. The annular array of air outlet holes all communicate with the second channel.

[0014] The present invention has the following advantages: 1. The present invention makes the sponge closely fit with the first microfiltration membrane through the inflation of the airbag, and then combines the impact of bubbles on the impurities attached to the inner side of the first microfiltration membrane, so that the device can clean the first microfiltration membrane without stopping the machine, saving a lot of time and greatly improving the purification rate of the biological enzyme.

[0015] 2. Through the docking of the flexible ring with the backwashing cylinder, the first microfiltration membrane is sealed and fitted between the flexible ring and the backwashing cylinder, and then the first piston shaft in the backwashing cylinder is used to backwash the first microfiltration membrane, improving the filtration rate of the first microfiltration membrane.

[0016] 3. The impurities in the inner cavity of the housing are collected through the second microfiltration membrane, preventing the accumulation of impurities in the inner cavity of the housing, which would otherwise lead to an increasing blockage frequency of the first microfiltration membrane and a decrease in its filtration rate.

[0017] 4. Bubbles are formed by introducing gas into the culture solution to impact the impurities therein, suspending them in the culture solution and preventing the impurities from depositing on the upper plane of the chassis where they cannot be collected. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a three-dimensional structural schematic diagram of the present invention;

[0019] Figure 2 is a three-dimensional structural sectional schematic diagram of the housing of the present invention;

[0020] Figure 3 is a three-dimensional structural sectional schematic diagram of the housing and the first microfiltration membrane of the present invention;

[0021] Figure 4 is a three-dimensional structural schematic diagram of the first push rod and the first sliding shaft of the present invention;

[0022] Figure 5 is a three-dimensional structural sectional schematic diagram of the airbag and the sponge of the present invention;

[0023] Figure 6 is a three-dimensional structural sectional schematic diagram of the rotating shaft of the present invention;

[0024] Figure 7 is a three-dimensional structural schematic diagram of the rotating shaft and the third gear of the present invention;

[0025] Figure 8 is a three-dimensional structural schematic diagram of the second push rod and the third push rod of the present invention;

[0026] Figure 9 is a three-dimensional structural schematic diagram of the second sliding shaft and the extrusion block of the present invention;

[0027] Figure 10 is a three-dimensional structural sectional schematic diagram of the backflush cylinder of the present invention;

[0028] Figure 11 is a three-dimensional structural sectional schematic diagram of the docking pipe of the present invention;

[0029] Figure 12 is a three-dimensional structural schematic diagram of the third sliding shaft of the present invention;

[0030] Figure 13 is a three-dimensional structural sectional schematic diagram of the third sliding shaft of the present invention.

[0031] Names of the reference numerals in the figure: 1: base, 2: outer shell, 3: control terminal, 4: first motor, 5: first gear, 6: rotating shaft, 7: second gear, 8: chassis, 9: top plate, 10: first microfiltration membrane, 11: first channel, 12: airbag, 13: sponge, 14: flexible tube, 15: air valve, 201: first push rod, 202: first sliding shaft, 203: sealing ring, 301: second motor, 302: rotating shaft, 303: third gear, 401: backwashing cylinder, 402: first piston shaft, 403: first elastic element, 501: second push rod, 502: second sliding shaft, 503: extrusion block, 504: trigger rod, 601: third push rod, 602: docking pipe, 603: piston disc, 604: second piston shaft, 605: flexible ring, 606: second elastic element, 701: fourth push rod, 702: third sliding shaft, 703: sealing disc, 704: fixed disc, 705: second microfiltration membrane, 706: feed hole, 707: second channel, 708: air outlet hole. Detailed implementation manners

[0032] Reference to an embodiment herein means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present invention. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0033] When microfiltration purifying bioenzymes, impurities in the culture solution will clog the pore diameter of the microfiltration membrane, resulting in a decrease in flow rate. Most of the existing cleaning methods require shutting down the machine to remove the microfiltration membrane for cleaning and then reinstalling the microfiltration membrane. This cleaning method not only takes time and effort but also greatly reduces the purification rate of bioenzymes.

[0034] Embodiment 1: An industrial bioenzyme purification device with a cleaning function, as Figures 1-6As shown in the figure, it includes a base 1. The base 1 is fixedly connected to a housing 2. A control terminal 3 is fixedly connected to the front side of the outside of the housing 2. A first motor 4 electrically connected to the control terminal 3 is fixedly connected to the top of the housing 2. A first gear 5 is fixedly connected to the output shaft of the first motor 4. A rotating shaft 6 located inside the housing 2 is rotatably connected to the top of the housing 2. A second gear 7 meshing with the first gear 5 is fixedly connected to the outside of the rotating shaft 6. The first motor 4 drives the rotating shaft 6 to rotate through the first gear 5 and the second gear 7. The bottom and the top of the inside of the housing 2 are respectively rotatably connected to a chassis 8 and a top plate 9. A first microfiltration membrane 10 is fixedly connected between the chassis 8 and the top plate 9. The first microfiltration membrane 10 is used to filter impurities in the culture solution. The chassis 8, the top plate 9 and the first microfiltration membrane 10 divide the housing 2 into two cavities, an inner cavity and an outer cavity. A first channel 11 is arranged inside the rotating shaft 6. The first channel 11 is communicated with an external gas injection device. An airbag 12 located inside the first microfiltration membrane 10 is fixedly connected to the rotating shaft 6. The first channel 11 inside the rotating shaft 6 is communicated with the airbag 12. A sponge 13 fixedly connected to the rotating shaft 6 is fixedly connected to the outside of the airbag 12, which is used to make the gas in the first channel 11 enter the airbag 12, so that the airbag 12 expands to drive the sponge 13 to closely fit with the first microfiltration membrane 10. The rotation of the rotating shaft 6 drives the sponge 13 to wipe off the impurities attached to the inside of the first microfiltration membrane 10. The rotating shaft 6 and the airbag 12 are jointly fixedly connected with flexible tubes 14 arranged in a linear array and mirror-symmetrically distributed. The flexible tubes 14 are used to adapt to the expansion and contraction of the airbag 12. The flexible tubes 14 arranged in a linear array and mirror-symmetrically distributed are all communicated with the first channel 11 inside the rotating shaft 6, which is used to make the gas inside the first channel 11 enter the inner cavity of the housing 2 along the flexible tubes 14 and the sponge 13, and then form bubbles to impact and clean the impurities attached to the inside of the first microfiltration membrane 10. An air valve 15 is arranged at the top of the housing 2. The air valve 15 is used to discharge the gas injected into the inner cavity of the housing 2, so as to stabilize the pressure inside the inner cavity of the housing 2. The bottom and the top of the housing 2 are respectively fixedly connected with a liquid inlet pipe and a liquid outlet pipe. The liquid inlet pipe is communicated with the inner cavity, which is used to inject the culture solution into the inner cavity of the housing 2. The liquid outlet pipe is communicated with the outer cavity, which is used to discharge the purified biological enzyme culture solution. A blocking assembly for blocking the airbag 12 is arranged on the rotating shaft 6. By the expansion of the airbag 12, the sponge 13 is closely attached to the first microfiltration membrane 10, and then combined with the impact of bubbles on the impurities attached to the inside of the first microfiltration membrane 10, the first microfiltration membrane 10 can be cleaned without stopping the machine, saving a lot of time and greatly improving the purification rate of biological enzymes.

[0035] As Figure 2 , Figure 3 and Figure 6As shown, the blocking component includes a first push rod 201 electrically connected to the control terminal 3, the first push rod 201 is fixedly connected to the top of the shell 2 through a connecting piece, the telescopic end of the first push rod 201 is fixedly connected to the first sliding shaft 202, the first sliding shaft 202 is fixedly connected to the linear array of blocking rings 203, the linear array of blocking rings 203 are all slidably connected to the rotating shaft 6, the telescopic end of the first push rod 201 drives the linear array of blocking rings 203 to move upward or downward through the first sliding shaft 202, the linear array of blocking rings 203 and the airbag 12 are blocked, and the blocking ring 203 does not block the airbag 12 in the initial state, and is used to inflate the airbag 12, the linear array of blocking rings 203 are respectively blocked with adjacent mirror-distributed flexible tubes 14, and the initial state is a blocked state, and a rotating component for rotating the first microfiltration membrane 10 is arranged inside the shell 2.

[0036] like Figure 1 and Figure 7 As shown, the rotating assembly includes a second motor 301 electrically connected to the control terminal 3, the second motor 301 is fixedly connected to the top of the housing 2 through a connecting piece, the output shaft of the second motor 301 is fixedly connected to the rotating shaft 302, the rotating shaft 302 is fixedly connected to two third gears 303 of a linear array, the output shaft of the second motor 301 drives the two third gears 303 to rotate through the rotating shaft 302, the outside of the chassis 8 and the top plate 9 are fixedly connected with teeth of an annular array, the chassis 8 and the top plate 9 are transmission connected to adjacent third gears 303 through the teeth of the annular array, the two third gears 303 respectively drive the chassis 8 and the top plate 9 to rotate synchronously, thereby driving the first microfiltration membrane 10 therebetween to rotate, thereby changing the fitting position of the first microfiltration membrane 10 and the sponge 13.

[0037] When the biological enzyme needs to be purified by microfiltration, the staff injects the culture solution into the inner cavity of the outer shell 2 through the liquid inlet pipe. After the culture solution enters the inner cavity of the outer shell 2, the biological enzyme in the culture solution passes through the first microfiltration membrane 10 and enters the outer cavity of the outer shell 2, and is then discharged along the liquid outlet pipe. At this time, the impurities in the culture solution will remain in the inner cavity of the outer shell 2, and adhere to the inner side of the first microfiltration membrane 10 to block it, thereby reducing the filtration efficiency of the first microfiltration membrane 10.

[0038] When microfiltration purifying the bio-enzyme, the staff turns on the external gas injection device. The gas injection device injects gas into the first channel 11 inside the rotating shaft 6. After the gas enters the first channel 11, it enters the airbag 12. The gas entering the inside of the airbag 12 causes it to expand. The expansion of the airbag 12 drives the external sponge 13 to fit with the first microfiltration membrane 10. When the airbag 12 expands until the sponge 13 and the first microfiltration membrane 10 are in a tightly contacting state, at this time, the first push rod 201 is turned on. The telescopic end of the first push rod 201 drives the first sliding shaft 202 to move upward. The upward movement of the first sliding shaft 202 drives the linear array of sealing rings 203 to move synchronously, so that the linear array of sealing rings 203 releases the sealing of the linear array and mirror-distributed flexible tubes 14 and seals the airbag 12. Then the first push rod 201 is turned off. At this time, the gas in the first channel 11 enters the flexible tube 14, enters the sponge 13 along the flexible tube 14, and then enters the culture solution in the inner cavity of the outer shell 2 to form bubbles to impact the impurities attached to the inner side of the first microfiltration membrane 10. The bubbles entering the inner cavity of the outer shell 2 move upward and finally are discharged along the air valve 15 at the top of the outer shell 2 to stabilize the pressure inside the outer shell 2. Then the first motor 4 is turned on. The output shaft of the first motor 4 drives the first gear 5 to rotate. The first gear 5 drives the rotating shaft 6 to rotate synchronously through the second gear 7. The rotating shaft 6 drives the sponge 13 to rotate synchronously to wipe the inner side of the first microfiltration membrane 10. The sponge 13 is tightly attached to the first microfiltration membrane 10 by the expansion of the airbag 12, and then combined with the bubbles to impact the impurities attached to the inner side of the first microfiltration membrane 10, so that the first microfiltration membrane 10 can be cleaned without stopping the machine, saving a lot of time and greatly improving the purification rate of the bio-enzyme.

[0039] When turning on the first motor 4, the second motor 301 is turned on. The output shaft of the second motor 301 drives the rotating shaft 302 to rotate synchronously. The rotation of the rotating shaft 302 drives the two third gears 303 thereon to rotate synchronously. The rotation of the third gears 303 drives the chassis 8 and the top plate 9 to rotate. The rotation of the chassis 8 and the top plate 9 drives the first microfiltration membrane 10 between them to rotate synchronously, thereby changing the contact position between the first microfiltration membrane 10 and the sponge 13, so that the sponge 13 evenly wipes the impurities on the inner side of the first microfiltration membrane 10.

[0040] So until the purification of the bio-enzyme is completed. At this time, the external gas injection device, the first motor 4 and the second motor 301 are turned off. Then the first push rod 201 is turned on. The telescopic end of the first push rod 201 drives the linear array of sealing rings 203 to reset through the first sliding shaft 202 to seal the flexible tube 14 again. At the same time, the gas in the airbag 12 is discharged from the first channel 11, and the airbag 12 shrinks to the initial state. When it is necessary to microfiltration purify the bio-enzyme again, repeat the above steps.

[0041] When the biocatalyst is being purified, when the first microfiltration membrane 10 filters the impurities in the culture solution, the impurities in the culture solution will not only adhere to the inner side of the first microfiltration membrane 10, but some impurities will enter the filtration pores of the first microfiltration membrane 10, which are difficult to clean and block the first microfiltration membrane 10 from the inside, resulting in a reduction in the filtration efficiency of the first microfiltration membrane 10.

[0042] Example 2: On the basis of Example 1, as Figures 8-10 shown, it further includes a backwashing assembly for backwashing the first microfiltration membrane 10. The backwashing assembly is arranged in the housing 2. The backwashing assembly includes six backwashing cylinders 401 arranged in a linear array. The six backwashing cylinders 401 arranged in a linear array are all fixedly connected to the outer cavity of the housing 2 through connecting pieces. A first piston shaft 402 is slidably connected in the backwashing cylinder 401. The first piston shaft 402 slides along the adjacent backwashing cylinder 401 to backwash the first microfiltration membrane 10. A first elastic element 403 is arranged between the first piston shaft 402 and the adjacent backwashing cylinder 401. The first elastic element 403 is a spring, which is used to drive the adjacent first piston shaft 402 to reset. A pushing assembly is arranged on the housing 2 for pushing the first piston shaft 402.

[0043] As Figures 8-10 shown, the pushing assembly includes a second push rod 501 electrically connected to the control terminal 3. The second push rod 501 is fixedly connected to the top of the housing 2 through a connecting piece. The telescopic end of the second push rod 501 is fixedly connected to a second sliding shaft 502. The second sliding shaft 502 is slidably connected to the housing 2. The second sliding shaft 502 is fixedly connected to six extrusion blocks 503 arranged in a linear array. The second push rod 501 drives the six extrusion blocks 503 arranged in a linear array thereon to slide up or down. The extrusion block 503 is a right-angled triangular plate, and the hypotenuse of the extrusion block 503 faces the adjacent backwashing cylinder 401. Six trigger rods 504 are fixedly connected to the six first piston shafts 402 arranged in a linear array. The six extrusion blocks 503 arranged in a linear array are respectively in extrusion cooperation with the adjacent trigger rods 504. The hypotenuse of the extrusion block 503 extrudes the adjacent trigger rod 504, so that the trigger rod 504 drives the adjacent first piston shaft 402 to slide along the adjacent backwashing cylinder 401.

[0044] As Figure 8 and Figure 11As shown in the figure, it further includes a docking component for docking the first microfiltration membrane 10 with the backflush cylinder 401. The docking component is arranged inside the first microfiltration membrane 10. The docking component includes a third push rod 601 electrically connected to the control terminal 3. The third push rod 601 is fixedly connected to the top of the housing 2 through a connecting member. A docking pipe 602 is fixedly connected to the top of the housing 2 and located inside it. A piston disc 603 slidably connected to the docking pipe 602 is fixedly connected to the telescopic end of the third push rod 601. The telescopic end of the third push rod 601 drives the piston disc 603 to slide up and down along the docking pipe 602, pushing the gas inside it. Six second piston shafts 604 in a linear array are slidably connected to the docking pipe 602. The docking pipe 602, the piston disc 603 and the six second piston shafts 604 in the linear array cooperate to form a sealed cavity. The gas in the docking pipe 602 pushes the six second piston shafts 604 in the linear array to slide outwards. The second piston shaft 604 is fixedly connected to a flexible ring 605. The central axis of the flexible ring 605 is collinear with the central axis of the adjacent backflush cylinder 401, for the second piston shaft 604 to drive the flexible ring 605 to dock with the adjacent backflush cylinder 401. A second elastic element 606 is arranged between each of the second piston shafts 604 in the linear array and the docking pipe 602. The second elastic element 606 is a spring, for driving the adjacent second piston shaft 604 to reset. By docking the flexible ring 605 with the adjacent backflush cylinder 401, the first microfiltration membrane 10 is fixed between the two, facilitating subsequent backflushing of the first microfiltration membrane 10.

[0045] When purifying the bioenzyme, when the staff observes that the flow rate of the liquid outlet pipe starts to decrease, the third push rod 601 is turned on, and at the same time, the second motor 301 is turned off. At this time, the telescopic end of the third push rod 601 drives the piston disc 603 to slide down along the docking pipe 602. The piston disc 603 pushes the gas in the docking pipe 602, so that the gas in the docking pipe 602 pushes the second piston shafts 604 in the linear array to slide outwards. At the same time, the second elastic elements 606 in the linear array are compressed. The second piston shaft 604 drives the flexible ring 605 thereon to slide synchronously, so that the flexible ring 605 pushes the first microfiltration membrane 10 to move until it fits with the adjacent backflush cylinder 401. Then the third push rod 601 is turned off, and the second push rod 501 is turned on. The second push rod 501 drives the extrusion blocks 503 in the linear array to slide down synchronously through the second sliding shaft 502. At this time, the extrusion blocks 503 extrude the adjacent trigger rods 504. The trigger rods 504 drive the adjacent first piston shafts 402 to slide along the adjacent backflush cylinder 401 under the extrusion force, and inject the culture solution inside it towards the first microfiltration membrane 10 for backwashing, and backflush the impurities attached to the filtration pores of the first microfiltration membrane 10 into the inner cavity of the housing 2.

[0046] After the culture solution in the recoil cylinder 401 is completely injected into the inner cavity of the outer shell 2, the second push rod 501 is closed at this time, and the third push rod 601 is opened, so that the telescopic end of the third push rod 601 drives the piston disk 603 thereon to reset. At this time, the linear array of second elastic elements 606 resets to drive the adjacent second piston shafts 604 to reset. The reset of the second piston shaft 604 drives the adjacent flexible ring 605 to separate from the adjacent recoil cylinder 401. At this time, the second motor 301 is turned on, so that the output shaft of the second motor 301 drives the first microfiltration membrane 10 to rotate through the third gear 303, the chassis 8 and the top plate 9, changing the recoil position of the recoil cylinder 401 on the first microfiltration membrane 10. Then the second motor 301 is turned off, and at the same time the second push rod 501 is turned on. The telescopic end of the second push rod 501 drives the linear array of extrusion blocks 503 to reset through the second sliding shaft 502. At this time, the linear array of first elastic elements 403 resets and drives the adjacent first piston shafts 402 to synchronously reset until the initial state. Then the second push rod 501 is turned off, and the above steps are repeated until the first microfiltration membrane 10 is uniformly backwashed for one circle.

[0047] During the purification process of the biological enzyme, as the biological enzyme is filtered in the inner cavity of the outer shell 2, the impurities in the inner cavity will increase more and more, which will gradually increase the blockage frequency of the first microfiltration membrane 10 and reduce the filtration rate of the first microfiltration membrane 10.

[0048] Embodiment 3: On the basis of Embodiment 2, as Figure 12 and Figure 13As shown in the figure, it further includes a collection component for collecting impurities. The collection component is arranged on the outer shell 2. The collection component includes a fourth push rod 701 electrically connected to the control terminal 3. The fourth push rod 701 is fixedly connected to the top of the outer shell 2 through a connecting piece. The telescopic end of the fourth push rod 701 is fixedly connected with a third sliding shaft 702. The third sliding shaft 702 is in contact and cooperation with the chassis 8. A plugging disk 703 is detachably connected to the top of the outer shell 2. The third sliding shaft 702 is slidably connected to the plugging disk 703. The telescopic end of the fourth push rod 701 drives the third sliding shaft 702 to slide up or down. A fixed disk 704 is fixedly connected to the lower part of the third sliding shaft 702. A second microfiltration membrane 705 is fixedly connected between the fixed disk 704 and the third sliding shaft 702. The second microfiltration membrane 705 is used to collect impurities in the inner cavity of the outer shell 2. The third sliding shaft 702, the fixed disk 704 and the second microfiltration membrane 705 cooperate to form a sealed cavity. The fixed disk 704 is fixedly connected with four feeding holes 706 arranged in an annular array. The feeding holes 706 are frustum-shaped, and the end with a smaller diameter of the feeding holes 706 is located at the lower end. The four feeding holes 706 arranged in an annular array are communicated with the sealed cavity, which is convenient for impurities in the inner cavity of the outer shell 2 to enter the sealed cavity, and at the same time, it is not convenient for the impurities in the sealed cavity to flow back into the inner cavity of the outer shell 2 again. The impurities in the inner cavity of the outer shell 2 are collected through the second microfiltration membrane 705, so as to avoid more and more impurities in the inner cavity of the outer shell 2, which will lead to a gradual increase in the blockage frequency of the first microfiltration membrane 10 and a decrease in the filtration rate of the first microfiltration membrane 10. A second channel 707 is arranged inside the third sliding shaft 702. The second channel 707 is communicated with an external gas injection device. Four air outlet holes 708 arranged in an annular array are arranged at the bottom of the third sliding shaft 702. The four air outlet holes 708 arranged in an annular array are all communicated with the second channel 707, so that the air outlet holes 708 inject gas into the inner cavity of the outer shell 2. The gas enters the culture solution to form bubbles to impact the impurities therein, so that the impurities are suspended in the culture solution, and it is avoided that the impurities are deposited on the upper plane of the chassis 8 and cannot be collected.

[0049] When the biological enzyme starts to be purified, the fourth push rod 701 is turned on. The telescopic end of the fourth push rod 701 drives the third sliding shaft 702 to slide down along the plugging disk 703. The third sliding shaft 702 drives the fixed disk 704 thereon to slide down synchronously. At this time, the culture solution in the inner cavity of the outer shell 2 will carry the impurities therein and enter the sealed cavity formed by the cooperation of the fixed disk 704, the third sliding shaft 702 and the second microfiltration membrane 705 along the four feeding holes 706 arranged in an annular array. This continues until the third sliding shaft 702 moves into contact with the chassis 8, and then the fourth push rod 701 drives the third sliding shaft 702 to slide up. This cycle is repeated, and the impurities in the inner cavity of the outer shell 2 are collected through the second microfiltration membrane 705, so as to avoid more and more impurities in the inner cavity of the outer shell 2, which will lead to a gradual increase in the blockage frequency of the first microfiltration membrane 10 and a decrease in the filtration rate of the first microfiltration membrane 10.

[0050] When impurities in the inner cavity of the second microfiltration membrane 705 collection housing 2 are collected, the staff turns on the external air injection device to inject air into the second channel 707 in the third sliding shaft 702. The gas in the second channel 707 flows downward and enters the four air outlet holes 708 in the annular array. The gas enters the inner cavity of the housing 2 from the four air outlet holes 708 in the annular array. The gas enters the culture solution to form bubbles to impact the impurities therein, making them suspended in the culture solution and preventing the impurities from depositing on the upper plane of the chassis 8, so that they cannot be collected.

[0051] So until the biocatalyst purification is completed, then the fourth push rod 701 drives the third sliding shaft 702 to reset to the initial state and closes, and the external air injection device is shut down. When the biocatalyst is purified again, the above steps are repeated.

[0052] The staff regularly removes the plugging disc 703 and cleans the impurities stored in the sealed cavity formed by the cooperation of the third sliding shaft 702, the fixed disc 704 and the second microfiltration membrane 705. After the cleaning is completed, the plugging disc 703 is installed on the top of the housing 2 again to prevent the impurities from filling the sealed cavity formed by the cooperation of the third sliding shaft 702, the fixed disc 704 and the second microfiltration membrane 705, resulting in the inability to collect the impurities in the inner cavity of the housing 2 in time and reducing the filtration rate of the first microfiltration membrane 10.

[0053] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. An industrial bio-enzyme purification device with a cleaning function, comprising a base (1), wherein the base (1) is fixedly connected to a housing (2), a control terminal (3) is fixedly connected to the outside of the housing (2), a first motor (4) electrically connected to the control terminal (3) is fixedly connected to the top of the housing (2) through a connecting member, an output shaft of the first motor (4) is fixedly connected to a first gear (5), a rotating shaft (6) is rotatably connected to the top of the housing (2), a second gear (7) meshing with the first gear (5) is fixedly connected to the outside of the housing (2) by the rotating shaft (6), a bottom plate (8) and a top plate (9) are rotatably connected to the bottom and top of the housing (2), respectively, a first microfiltration membrane (10) is fixedly connected between the bottom plate (8) and the top plate (9), the bottom plate (8), the top plate (9) and the first microfiltration membrane (10) divide the housing (2) into an inner cavity and an outer cavity, a first channel (11) is arranged inside the rotating shaft (6), and the device is characterized in that: It also comprises an airbag (12), the airbag (12) being fixedly connected to the rotating shaft (6), the first channel (11) inside the rotating shaft (6) being in communication with the airbag (12), the outside of the airbag (12) being fixedly connected with a sponge (13) fixedly connected to the rotating shaft (6), the rotating shaft (6) and the airbag (12) being jointly fixedly connected with a linear array of flexible tubes (14) distributed in a mirror image, the flexible tubes (14) being in communication with the first channel (11), the top of the housing (2) being provided with an air valve (15), the bottom and top of the housing (2) being respectively fixedly connected with a liquid inlet tube in communication with the inner cavity and a liquid outlet tube in communication with the outer cavity, and the rotating shaft (6) being provided with a blocking component for blocking the airbag (12); The blocking component comprises a first push rod (201) electrically connected to the control terminal (3); the first push rod (201) is fixedly connected to the top of the housing (2) via a connecting piece; the telescopic end of the first push rod (201) is fixedly connected to a first sliding shaft (202); the first sliding shaft (202) is fixedly connected to a linear array of blocking rings (203); the linear array of blocking rings (203) are all slidably connected to the rotating shaft (6); the linear array of blocking rings (203) are in blocking cooperation with the airbags (12); the linear array of blocking rings (203) are respectively in blocking cooperation with the adjacent mirror-distributed flexible tubes (14); and a rotating component for rotating the first microfiltration membrane (10) is provided inside the housing (2); The rotating assembly comprises a second motor (301) electrically connected to the control terminal (3); the second motor (301) is fixedly connected to the top of the housing (2) via a connecting piece; the output shaft of the second motor (301) is fixedly connected to the rotating shaft (302); the rotating shaft (302) is fixedly connected to a third gear (303) in a linear array; the outsides of the chassis (8) and the top plate (9) are fixedly connected to teeth in an annular array; the chassis (8) and the top plate (9) are both transmission-connected to the adjacent third gear (303) via the teeth in the annular array; The invention also comprises a backwashing assembly for backwashing the first microfiltration membrane (10), the backwashing assembly being arranged in the housing (2), the backwashing assembly comprising a recoil cylinder (401) in a linear array, the recoil cylinders (401) in the linear array being fixedly connected in the housing (2) via a connecting piece, a first piston shaft (402) being slidably connected in the recoil cylinder (401), a first elastic element (403) being arranged between the first piston shaft (402) and the adjacent recoil cylinder (401), and a pushing assembly for pushing the first piston shaft (402) being arranged on the housing (2); The pushing assembly comprises a second push rod (501) electrically connected to the control terminal (3); the second push rod (501) is fixedly connected to the top of the housing (2) via a connecting piece; the telescopic end of the second push rod (501) is fixedly connected to a second sliding shaft (502); the second sliding shaft (502) is slidably connected to the housing (2); the second sliding shaft (502) is fixedly connected to a linear array of extrusion blocks (503); the first piston shafts (402) of the linear array are all fixedly connected to trigger rods (504); the extrusion blocks (503) of the linear array are respectively extruded and matched with adjacent trigger rods (504); The invention also comprises a docking assembly for docking the first microfiltration membrane (10) with the recoil cylinder (401), the docking assembly being arranged inside the first microfiltration membrane (10), the docking assembly comprising a third push rod (601) electrically connected to the control terminal (3), the third push rod (601) being fixedly connected to the top of the housing (2) via a connecting piece, the housing (2) being fixedly connected to a docking tube (602) located inside the housing (2), the telescopic end of the third push rod (601) being fixedly connected to a piston disc (603) slidably connected to the docking tube (602), the docking tube (602) being slidably connected to a second piston shaft (604) of a linear array, the second piston shaft (604) being fixedly connected to a flexible ring (605), and a second elastic element (606) being arranged between each of the second piston shafts (604) of the linear array and the docking tube (602).

2. The industrial bio-enzyme purification device with cleaning function according to claim 1 is characterized in that: The extrusion block (503) is a right-angled triangle plate, and the hypotenuse of the extrusion block (503) faces the adjacent recoil cylinder (401).

3. The industrial bio-enzyme purification device with cleaning function according to claim 1 is characterized in that: The invention also comprises a collection component for collecting impurities, the collection component being arranged on the housing (2), the collection component comprising a fourth push rod (701) electrically connected to the control terminal (3), the fourth push rod (701) being fixedly connected to the top of the housing (2) via a connecting piece, the telescopic end of the fourth push rod (701) being fixedly connected to a third sliding shaft (702), the third sliding shaft (702) being in contact with and mating with the chassis (8), the top of the housing (2) being detachably connected to a blocking disk (703), the third sliding shaft ( The third sliding shaft (702) is slidably connected to the blocking disk (703), the lower part of the third sliding shaft (702) is fixedly connected to a fixed disk (704), a second microfiltration membrane (705) is fixedly connected between the fixed disk (704) and the third sliding shaft (702), the third sliding shaft (702), the fixed disk (704) and the second microfiltration membrane (705) cooperate to form a closed cavity, the fixed disk (704) is fixedly connected to a circular array of feed holes (706), and the circular array of feed holes (706) are connected to the closed cavity.

4. The industrial bio-enzyme purification device with cleaning function according to claim 3 is characterized in that: The feed hole (706) is truncated cone-shaped, and the end of the feed hole (706) with a smaller diameter is located inside a closed cavity formed by the cooperation of the third sliding shaft (702), the fixed disk (704) and the second microfiltration membrane (705).

5. The industrial bio-enzyme purification device with cleaning function according to claim 4 is characterized in that: A second channel (707) is provided inside the third sliding shaft (702), and an annular array of air outlet holes (708) is provided at the bottom of the third sliding shaft (702), and the annular array of air outlet holes (708) are all in communication with the second channel (707).

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