A cell culture bag

Abstract

The present invention relates to a cell culture bag, comprising a bag body, a vent plate, a stirring mechanism, and a plurality of pipeline interfaces, wherein the vent plate is located below the bag body, the stirring mechanism is located in the bag body, and the plurality of pipeline interfaces are used for liquid communication between the inside and outside of the bag body, the stirring mechanism comprises a stirring rod, a stirring paddle, and a key, the stirring paddle comprises a shaft sleeve and a paddle, the paddle is located at the outer periphery of the shaft sleeve, a first strip groove extending along an axial direction is provided on the inner periphery of the shaft sleeve, a second strip groove is provided on the stirring rod, the key is in an elongated shape, and the length of the key is the same as the length of the second strip groove; the key has a first surface and a second surface that are arranged oppositely, and protrusions are respectively provided at the upper and lower ends of the first surface, so as to form a concave surface in the middle of the first surface, and the length of the concave surface is the same as the length of the shaft sleeve; after the key is placed in the second strip groove, the concave surface protrudes from the outer periphery of the stirring rod so as to be combined with the first strip groove.

Description

Technical Field

[0001] The invention relates to the field of cell culture, and in particular to a cell culture bag. Background Art

[0002] Bioreactor refers to an appropriate environment or engineering equipment that provides biochemical reactions. It usually refers to the use of enzymes or organisms (microorganisms) to make the device have the function of simulating organisms, and can carry out biochemical reactions outside the cell. In the simulation process, both aerobic and anaerobic reactions can be carried out. Based on the source, it can be divided into natural bioreactors and engineered bioreactors. Bioreactors are usually cylindrical, with a volume ranging from a few liters to a few cubic meters, and are often made of stainless steel, borosilicate glass or plastic bags.

[0003] The cell culture bag generally includes a bag body, a stirring paddle is arranged inside the bag body, a vent plate and a drain port are arranged at the bottom of the bag body, and the existing stirring paddle is usually fixed to the rotating shaft by screws or by a composite buckle structure. When the cell culture bag is used for cell culture, if the culture cycle is long, there is a risk of loosening of the screw fixing the stirring paddle, and there will be a large dead zone volume in the gap between the screw and the thread. The composite buckle has a complex structure, is difficult to connect and install, and cannot be disassembled after installation, has high requirements for operation, and is also costly.

[0004] The bottom of the cell culture bag is usually provided with a ventilation plate to provide oxygen or air to the culture bag during culture. The ventilation plate of the prior art is generally designed with 6, 8 or 10 ventilation interfaces at the bottom of the ventilation plate, and the gas enters the ventilation plate from these ventilation interfaces and diffuses into the culture solution. This type of ventilation plate has many ventilation interfaces, resulting in complex ventilation pipelines, difficult installation, and high costs. At the same time, when the operator connects the external gas pipeline at the interface or the bag body is hit by external force, the rotational pressure generated can easily cause the interface connection to loosen, which can easily lead to the risk of non-sealing of the culture bag, thereby causing contamination of the culture. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a cell culture bag, comprising a bag body, a vent plate, a stirring mechanism, and a plurality of pipeline interfaces, wherein the vent plate is located below the bag body, the stirring mechanism is located inside the bag body, and the plurality of pipeline interfaces are used for liquid communication between the inside and outside of the bag body.

[0006] The stirring mechanism comprises a stirring rod, a stirring paddle and a key. The stirring paddle comprises a sleeve and a paddle. The paddle is located at the outer periphery of the sleeve. The inner periphery of the sleeve is provided with a first strip groove extending along the axial direction. The stirring rod is provided with a second strip groove. The key is in the shape of a long strip, and the length of the key is the same as the length of the second strip groove.

[0007] The key has a first surface and a second surface that are arranged opposite to each other, and protrusions are respectively arranged at the upper and lower ends of the first surface, so as to form a recessed surface in the middle of the first surface, and the length of the recessed surface is the same as the length of the sleeve; after the key is placed in the second strip groove, the recessed surface protrudes from the outer periphery of the stirring rod to facilitate combination with the first strip groove.

[0008] Furthermore, the second surface is in a stepped shape, so that the key forms an elastic cantilever with a smaller thickness at the upper side than at the lower side, or the key forms an elastic cantilever with a smaller thickness at the lower side than at the upper side.

[0009] Furthermore, on the first surface, the protrusion located on the elastic cantilever has an inclined surface inclined from the end of the key to the middle and the outside of the key.

[0010] Furthermore, the cell culture bag is also provided with a driving device interface, and the driving device interface is used to connect an external driving device to drive the stirring mechanism to rotate.

[0011] Furthermore, the vent plate includes a base, a side wall, a protrusion, and an annular cover plate, wherein the protrusion is arranged on the base and protrudes from the base, the side wall is arranged around the base and extends upward, an annular cavity is formed between the protrusion and the side wall, and the annular cover plate is used to close the upper opening of the annular cavity. The annular cover plate is also provided with a plurality of pores, which are connected to the interior of the cell culture bag and the annular cavity.

[0012] Furthermore, a first countersunk hole is arranged in the annular cavity, and a second countersunk hole is arranged on the protruding seat, and the first countersunk hole and the second countersunk hole pass through the base.

[0013] Further, a first fastener is arranged in the first countersunk hole, and a second fastener is arranged in the second countersunk hole; the first fastener and the second fastener respectively have an integrally formed head and a rod, and the first fastener and the second fastener have a hollow channel;

[0014] The head of the first fastener cooperates with the countersunk head of the first countersunk hole, the head of the second fastener cooperates with the countersunk head of the second countersunk hole, and the stems of the first fastener and the second fastener pass through the base.

[0015] Furthermore, a thread is provided at the lower portion of the rod, which cooperates with a nut to fix the first fastener and the second fastener to the base; a pipeline interface is also provided at the outer periphery of the end of the rod.

[0016] Further, at least one first sealing ring is arranged between the lower part of the head of the first fastener and the first countersunk hole, and / or at least one second sealing ring is arranged between the stem of the first fastener and the first countersunk hole.

[0017] Further, at least one third sealing ring is arranged between the lower part of the head of the second fastener and the second countersunk hole, and / or at least one fourth sealing ring is arranged between the stem of the second fastener and the second countersunk hole.

[0018] The paddle installation of the present invention adopts a simple single buckle connection, which is simple and reliable, and the installation operation is simple. The single buckle is a low value-added loss part. When disassembling, the integrity and detachability of the paddle can be ensured by damaging the single buckle. The bottom of the ventilation plate at the bottom of the cell culture bag is only provided with a ventilation interface and a liquid interface, which can ensure that the gas of the ventilation plate is expanded from all sides into the culture medium. The structure is compact, the pipeline is simple, the operation is convenient, and the cost is also reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a diagram of the structure of the cell culture bag;

[0020] Figure 2 This is the disassembly diagram of the main components of the stirring mechanism;

[0021] Figure 3 It is a schematic diagram of the installation of the stirring mechanism;

[0022] Figure 4 It is an exploded view of the vent plate structure;

[0023] Figure 5 This is a structural diagram of the vent disc after assembly;

[0024] Figure 6 is a cross-sectional view of the vent disc. DETAILED DESCRIPTION

[0025] See also Figure 1 The cell culture bag of the present invention comprises a bag body 1, a vent plate 2, a stirring mechanism 3, a plurality of pipeline interfaces 4, and a driving device interface 5. The vent plate 2 is located below the bag body 1, the stirring mechanism 3 is located inside the bag body 1, and the plurality of pipeline interfaces 4 are used for liquid communication between the inside and outside of the culture bag. The driving device interface 5 is used for connecting an external driving device to drive the stirring mechanism 3 to rotate. The driving interface device 5 is preferably arranged above the culture bag, and can also be arranged below the culture bag.

[0026] See also Figure 2 The stirring mechanism 3 of the present invention includes a stirring rod 31, a stirring paddle 32, and a key 33. The stirring paddle 32 includes a sleeve 321 and a blade 322. The blade 322 is located on the outer periphery of the sleeve 321. The inner periphery of the sleeve 321 is provided with a first strip groove 3211 extending along the axial direction.

[0027] The stirring rod 31 is provided with a second strip groove 311, the key 33 is in a long strip shape, the length of the key 33 is the same as the length of the second strip groove 311, and the width of the key 33, the width of the first strip groove 3211, and the width of the second strip groove 311 are the same;

[0028] The key 33 has a first surface 331 and a second surface 332 that are oppositely disposed. The first surface 331 is provided with protrusions 333 at both ends, so that a concave surface 334 is formed in the middle of the first surface 331. The length of the concave surface 334 is the same as the length of the sleeve 321. After the key 33 is placed in the second strip groove 311, the concave surface 334 protrudes from the outer periphery of the stirring rod 31 so as to be combined with the first strip groove 3211.

[0029] The second surface 332 is stepped, so that the key 33 forms an elastic cantilever with a smaller thickness at the top or bottom (at Figure 2 The step shape may be a step transitioning from a vertical plane or a step transitioning from a vertical plane to a vertical plane. Figure 2 The steps shown use inclined surface transitions, and different transition forms make the elastic cantilever have different compression resistance effects. On the first surface 331, the protrusion 333 located on the cantilever has an inclined surface that tilts from the end of the key 33 to the middle of the key and toward the outside (the first surface 331 is the outside, and the second surface 332 is the inside).

[0030] See also Figure 3 , is a schematic diagram after the stirring mechanism of the present invention is installed. The process of installing the stirring paddle 32 is as follows: place the key 33 in the second strip groove 311, and then insert the sleeve 321 from one side of the stirring rod 31. In the figure, since the elastic cantilever is located at the upper part, the sleeve 321 is inserted downward from the upper part of the stirring rod, and the first strip groove 3211 is also aligned with the key 33. When the sleeve 321 contacts the key 33, due to the guidance of the inclined surface of the upper end protrusion 333, the elastic cantilever is squeezed and elastically deformed into the second strip groove 311, and the sleeve 321 can continue to move downward. When the lower end of the sleeve 321 abuts against the protrusion at the lower end of the key 33, the elastic cantilever rebounds, thereby firmly fixing the sleeve 321 in the recessed surface 334 and preventing it from moving up and down. At the same time, since a part of the key 33 is accommodated in the first strip groove 3211, the sleeve 321 cannot rotate relative to the stirring rod 31.

[0031] See also Figure 4-5 The vent plate 2 includes a base 21 , a side wall 22 , a protruding seat 23 , and an annular cover plate 25 .

[0032] The protrusion 23 is arranged on the base 21 and protrudes from the base 21. The side wall 22 is arranged around the base 21 and extends upward. An annular cavity 24 is formed between the protrusion 23 and the side wall 22. The annular cover plate 25 has the same shape as the annular cavity 24, thereby closing the upper opening of the annular cavity 24. A plurality of air holes 26 are also provided on the annular cover plate 25, thereby communicating with the annular cavity 24.

[0033] A first countersunk hole 28 is provided in the annular cavity 24 , and a second countersunk hole 27 is provided on the protruding seat 23 .

[0034] A first fastener is arranged in the first countersunk hole 28. The first fastener has an integrally formed head 281 and a stem 282. The shape of the head 281 is the same as the countersunk shape of the first countersunk hole 28. The stem 282 extends along the head 281 and passes through the first countersunk hole 28. The lower part of the stem 282 is provided with a thread, so that the first fastener is fixedly connected to the base 21 by a nut. The first fastener has a hollow passage, and a pipeline interface (such as a snap-on type) is arranged at the outer periphery of the end of the stem 282 to facilitate communication with an external air intake pipe. A first sealing ring 283 is arranged between the lower part of the head 281 and the first countersunk hole 28, and a second sealing ring 284 is arranged between the stem 282 and the first countersunk hole 28. An annular groove for accommodating the second sealing ring 284 is arranged in the base 21 at the position of the second sealing ring 284.

[0035] In the present invention, the shape of the head 281 is the same as the countersunk shape of the first countersunk hole 28, and is not circular, so as to facilitate the positioning and installation of the first fastener, and will not rotate at will. At the same time, the double sealing ring design is adopted, so that the sealing between the base and the external fluid is significantly enhanced. In terms of pipeline design, due to the use of the form of an annular cavity 24, the entire structure only needs two interfaces, one of which leads to the center of the ventilation disk for the discharge of the liquid in the cell culture bag, and the other interface is directly connected to the annular cavity 24, and the air holes on the annular cover plate 25 are connected to the inside of the cell culture bag, which not only makes the ventilation more uniform, but also greatly simplifies the structure of the system and the external ventilation pipeline design.

[0036] See also Figure 5-6 The second countersunk hole 27 is provided with a second fastener, and the structure and installation method of the second fastener are similar to those of the first fastener, and will not be described in detail. The base 21 is only changed into a protruding seat 23, and the hollow channel of the second fastener is used to discharge the liquid in the culture bag.

[0037] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cell culture bag, comprising a bag body (1), a venting plate (2), a stirring mechanism (3), and a plurality of pipeline interfaces (4), wherein the venting plate (2) is located below the bag body (1), the stirring mechanism (3) is located inside the bag body (1), and the plurality of pipeline interfaces (4) are used for liquid communication between the inside and outside of the bag body, characterized in that: The stirring mechanism (3) comprises a stirring rod (31), a stirring paddle (32), and a key (33); the stirring paddle (32) comprises a shaft sleeve (321) and a paddle (322); the paddle (322) is located on the outer periphery of the shaft sleeve (321); the inner periphery of the shaft sleeve (321) is provided with a first strip groove (3211) extending along the axial direction; the stirring rod (31) is provided with a second strip groove (311); the key (33) is in the shape of a long strip; and the length of the key (33) is the same as the length of the second strip groove (311); The key (33) comprises a first surface (331) and a second surface (332) which are arranged opposite to each other. The first surface (331) is provided with protrusions (333) at the upper and lower ends respectively, so that a recessed surface (334) is formed in the middle of the first surface (331). The length of the recessed surface (334) is the same as the length of the shaft sleeve (321). After the key (33) is placed in the second strip groove (311), the recessed surface (334) protrudes from the outer periphery of the stirring rod (31) so as to be combined with the first strip groove (3211). The second surface (332) is stepped, so that the key (33) forms an elastic cantilever with a smaller thickness at the top relative to the bottom, or forms an elastic cantilever with a smaller thickness at the bottom relative to the top.

2. The cell culture bag according to claim 1, characterized in that: On the first surface (331), the protrusion (333) located on the elastic cantilever has an inclined surface that slopes from the end of the key (33) toward the middle and the outside of the key.

3. The cell culture bag according to claim 1, characterized in that: The cell culture bag is also provided with a drive device interface (5), and the drive device interface (5) is used to connect an external drive device to drive the stirring mechanism (3) to rotate.

4. The cell culture bag according to claim 1, characterized in that: The vent plate (2) comprises a base (21), a side wall (22), a protruding seat (23), and an annular cover plate (25). The protruding seat (23) is arranged on the base (21) and protrudes from the base (21); the side wall (22) is arranged around the base (21) and extends upward; an annular cavity (24) is formed between the protruding seat (23) and the side wall (22); the annular cover plate (25) is used to close the upper opening of the annular cavity (24); a plurality of pores (26) are further arranged on the annular cover plate (25); the pores (26) are in communication with the interior of the cell culture bag and the annular cavity (24).

5. The cell culture bag according to claim 4, characterized in that: A first countersunk hole (28) is provided in the annular cavity (24), and a second countersunk hole (27) is provided on the protruding seat (23); the first countersunk hole (28) and the second countersunk hole (27) penetrate the base (21).

6. The cell culture bag according to claim 5, characterized in that: A first fastener is arranged in the first countersunk hole (28), and a second fastener is arranged in the second countersunk hole (27); the first fastener and the second fastener respectively have an integrally formed head (281) and a rod (282), and the first fastener and the second fastener have a hollow channel; The head of the first fastener cooperates with the countersunk head of the first countersunk hole (28), the head of the second fastener cooperates with the countersunk head of the second countersunk hole (27), and the stems of the first fastener and the second fastener pass through the base (21).

7. The cell culture bag according to claim 6, characterized in that: The lower part of the rod (282) is provided with a thread, which cooperates with a nut to fix the first fastener and the second fastener to the base (21); a pipeline interface is also provided on the outer periphery of the end of the rod (282).

8. The cell culture bag according to claim 6, characterized in that: At least one first sealing ring (283) is provided between the lower part of the head of the first fastener and the first countersunk hole (28), and / or at least one second sealing ring (284) is provided between the stem (282) of the first fastener and the first countersunk hole (28).

9. The cell culture bag according to claim 6, characterized in that: At least one third sealing ring is provided between the lower part of the head of the second fastener and the second countersunk hole (27), and / or at least one fourth sealing ring is provided between the stem of the second fastener and the second countersunk hole (27).

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