Application of tannic acid metal complexes in rice seed treatment

Abstract

The invention discloses an application of a tannic acid metal complex in rice seed treatment. The application is the application of the tannic acid metal complex as a rice seed treatment agent in improving the germination rate of rice seeds, the rice seedling rate and promoting the growth of rice seedlings. The tannic acid metal complex is zinc tannate and / or manganese tannate. Experiments have shown that the application of manganese tannate and zinc tannate in rice seed treatment can improve the germination rate, seedling rate and growth of rice seedlings of direct-seeded rice seeds, ensure the healthy growth of rice seedlings, and provide support for drone sowing and simplified rice cultivation.

Description

Technical Field

[0001] The invention relates to the field of seed treatment, and specifically to the application of tannic acid metal complexes in rice seed treatment; in particular, it relates to the application of tannic acid metal complexes in rice seed treatment to improve rice seed germination rate, seedling rate and promote rice seedling growth. Background Art

[0002] From sowing to harvesting, crop cultivation has increasingly higher requirements for intelligent, efficient, mechanized and simplified production, and urgently needs high-efficiency, labor-saving sowing and pesticide application technologies and products that match the crop planting model. Rice is the largest staple food consumed by Chinese residents, accounting for 60% of the food ration. Healthy seedlings are the key to ensuring food production, and breaking seed dormancy and preventing and controlling seed-borne diseases such as seedling disease and seedling rot are the key to seed treatment during rice sowing. At present, the main rice cultivation model first requires seedling cultivation, which generally requires seed drying, seed selection, seed soaking, germination, germination and sowing, and then planting in the nursery field to promote the rapid and uniform emergence of seeds, reduce the occurrence of seedling diseases, facilitate rooting in the seedling stage, and prevent rotten buds and dead seedlings. However, this planting method is cumbersome, has a long cycle, and a low level of mechanization. In particular, a large amount of wastewater is generated during the pesticide soaking process, which causes serious pollution to the environment.

[0003] The rising labor cost in rural areas has challenged the traditional rice planting method. Mechanization and simplification are the inevitable trends of rice production in the future. Direct seeding of rice is a method of directly sowing rice seeds in the field without sterilizing seedlings or transplanting. It includes dry direct seeding, water direct seeding and wet direct seeding, among which wet direct seeding is the main method. Direct seeding of rice can effectively reduce production costs, reduce labor consumption and improve rice planting efficiency. However, the current direct seeding of rice mainly faces bottleneck problems such as low germination rate, uneven emergence of seedlings, weak growth and easy lodging of seedlings. It is urgent to develop substances that can break seed dormancy to improve the germination rate of rice seeds and improve the quality of seedlings. This substance can be used with pesticides for disease control through seed coating, seed mixing, pelleting and other methods to treat seeds at one time, and can be applied to modern seeding machinery and equipment including drone sowing, which can greatly reduce the cumbersome production process such as seedling cultivation and realize the simplification of rice sowing. Summary of the invention

[0004] Based on the above problems, the first purpose of the present invention is to provide an application of tannic acid metal complex in improving the germination rate and seedling rate of rice seeds and promoting the growth of rice seedlings.

[0005] Tannic acid, also known as tannic acid, molecular formula C 76 H 52 O 46 , English name Tannic acid, CAS number 1401-55-4.

[0006] The method for obtaining the tannic acid metal complex is as follows: mixing a tannic acid aqueous solution with a zinc salt aqueous solution or a manganese salt aqueous solution, stirring and reacting for 3 hours to obtain the tannic acid metal complex; wherein the molar ratio of tannic acid to zinc salt or manganese salt is 1:20-30.

[0007] As a preferred technical solution of the present invention, the zinc salt is Zn(CH3COO)2, ZnSO4, ZnCl2, Zn(NO3)2; the manganese salt is MnSO4, MnCl2, Mn(NO3)2.

[0008] More preferably, the zinc salt is Zn(CH3COO)2; the manganese salt is preferably MnSO4;

[0009] Furthermore, in the method for obtaining the tannic acid metal complex, the molar ratio of the tannic acid to the zinc salt or manganese salt is preferably 1:27.5.

[0010] The tannic acid metal complex is used for seed treatment and has a good effect on improving the germination rate and seedling rate of rice seeds and promoting the growth of rice seedlings.

[0011] At a seed treatment dosage of 0.1-0.4 g / 100 kg, tannic acid metal complexes can increase the germination rate by 6%-16%, the seedling rate by 0.7%-12%, and the rice plant height by 15.35%-81.16%.

[0012] Preferably, the dosage of the tannic acid metal complex for rice seed treatment is 0.3-0.4 g / 100 kg of seeds.

[0013] Among them, manganese tannate can increase the germination rate of rice seeds by 14.0%-16.0%, the seedling rate by 8.7%-12.0%, and the plant height by 69.17%-81.16% at a seed treatment dose of 0.3-0.4 g / 100 kg of seeds. Zinc tannate can increase the germination rate of rice seeds by 10.0%-13.0%, the seedling rate by 4.0%-11.3%, and the plant height by 23.55%-55.61% at a seed treatment dose of 0.3-0.4 g / 100 kg of seeds.

[0014] The advantages of the present invention are:

[0015] 1. Tannic acid metal complexes can increase the germination rate of rice seeds, promote the formation of rice seedlings and promote the growth of rice seedlings (increase the height of rice plants), thereby enhancing the quality of rice seedlings and ensuring the health of rice seedlings.

[0016] 2. Tannic acid is environmentally friendly. The present invention provides a new use of the compound, and the tannic acid metal complex can be used as a seed treatment agent for direct seeding of rice seeds. DETAILED DESCRIPTION

[0017] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0018] In the following embodiments, the “parts by mass” mentioned herein is a unit of weight according to the understanding in the art, and may be “g”, “kg”, “mg”, “µg”, etc.

[0019] The reagents involved in the following embodiments and test examples are disclosed as follows:

[0020] Tannic acid (96%, Tianjin Xiens Biochemical Technology Co., Ltd.), KCl (99.5%, Sinopharm Chemical Reagent Co., Ltd.), MgSO4 (99%, Tianjin Fuchen Chemical Reagent Factory), Zn(CH3COO)2 (98%, Shanghai McLean Biochemical Technology Co., Ltd.), CaCl2 (96%, Sinopharm Chemical Reagent Co., Ltd.), Fe2(SO4)3 (96%, Tianjin Xiens Biochemical Technology Co., Ltd.), MnSO4 (99%, Tianjin Xiens Biochemical Technology Co., Ltd.).

[0021] The rice seeds were Song F17, a conventional japonica rice.

[0022] Example 1: Application of tannic acid metal complex in improving rice germination rate

[0023] 1. Preparation of Tannic Acid Metal Complex

[0024] Dissolve tannic acid in deionized water to prepare a 0.012 mol / L tannic acid solution; dissolve KCl, CaCl2, MgSO4, Zn(CH3COO)2, MnSO4 and Fe2(SO4)3 in deionized water to prepare a 0.33 mol / L metal salt solution; mix the tannic acid solution and the metal salt solution in a volume ratio of 1:1, stir for 3 hours, and dry to remove moisture after the reaction is completed to obtain a tannic acid metal complex.

[0025] 2. Rice Seed Treatment

[0026] Preparation of rice seed treatment agent: 5 mL of deionized water was measured, mixed with 0.2-0.8 mg of tannic acid, metal salt or tannic acid metal complex prepared in step 1, and 0.25 g of film-forming agent (styrene-acrylate emulsion, solid content 53%, Beijing Yite Agricultural Technology Extension Service Co., Ltd., film-forming agent 11-1#) was added respectively to prepare rice seed treatment agents with tannic acid, metal salt or tannic acid metal complex prepared in step 1 as active ingredients, and an equal amount of water was used to replace the active ingredient to prepare a control treatment agent in parallel.

[0027] Seed treatment method: In a culture dish, according to the effective ingredient dosage of 0.1-0.4 g / 100 kg seeds (for specific dosage, please see Table 1 and Table 2), weigh 1 g of the above rice seed treatment agent or control treatment agent, and mix it thoroughly with 40 g of rice seeds Song F17 to obtain coated rice seeds.

[0028] 3. Rice sowing and cultivation method

[0029] The rice seed germination rate detection method in the germination test of GB / T 3543.4-1995 Inspection Procedure for Seeds of Crops was adopted. The rice seeds were treated with rice seed treatment agents with tannic acid, metal salts or tannic acid metal complexes as active ingredients, and the control treatment agent coating was used as the control treatment. Twenty-five rice seeds were placed in a 9 cm culture dish using the paper bed method, and repeated 4 times. 5 mL of deionized water was evenly added to each dish, and the seeds were cultured in a dark moisturizing incubator at 25°C.

[0030] IV. Survey Methods

[0031] After 14 days of cultivation, the number of germinations in each treatment was investigated and recorded, and the germination rate and germination rate increase were calculated according to the following formula:

[0032]

[0033] V. Test Results

[0034] After the rice seed treatment agent with tannic acid, metal salt or tannic acid metal complex prepared in step 1 as the effective ingredient was treated, the results of the rice seed germination rate were shown in Tables 1 and 2. Compared with the control group, MnSO4 at a dosage of 0.1-0.4 g / 100 kg seeds can increase the rice seed germination rate by 10.0%-17.0%; Fe2(SO4)3 at a dosage of 0.1-0.4 g / 100 kg seeds can increase the rice seed germination rate by 8.0%-13.0%; Zn(CH3COO)2 and KCl at a dosage of 0.2-0.4 g / 100 kg seeds can increase the rice seed germination rate by 2.0%-14.0% and 2.0%-4.0%. Tannic acid at a dosage of 0.1-0.4 g / 100 kg seeds has no promoting effect on seed germination or the effect is not significant. Tannic acid magnesium, tannic acid manganese, tannic acid iron and tannic acid zinc, at a dosage of 0.1-0.4 g / 100 kg of seeds, all promote the germination of rice seeds, and can increase the germination rate by more than 6%, up to 16.0%. Among them, after treatment with tannic acid manganese and tannic acid zinc, the germination rate of rice seeds increased by 6.0%-16.0% and 6.0%-13.0%, respectively. In particular, at a seed treatment dosage of 0.3-0.4 g / 100 kg of seeds, tannic acid manganese can increase the germination rate of rice seeds by 14.0%-16.0%, and tannic acid zinc can increase the germination rate of rice seeds by 10.0%-13.0%, which is the best performance.

[0035] Table 1. Effects of single-agent treatments of tannic acid and metal salts on germination rate of rice seeds

[0036]

[0037] Table 2. Effects of tannic acid metal complex treatment on rice seed germination rate

[0038]

[0039] Example 2: Application of tannic acid metal complex in improving rice seedling rate

[0040] 1. Preparation of Tannic Acid Metal Complex

[0041] The preparation method of tannic acid metal complex is the same as that in Example 1.

[0042] 2. Rice Seed Treatment

[0043] Preparation of rice seed treatment agent: Referring to the method of Example 1, tannic acid, metal salt and tannic acid metal complex or control treatment agent are mixed with film-forming agent and water in a certain proportion to prepare seed treatment agent.

[0044] Seed treatment method: Referring to the method of Example 1, a rice seed treatment agent or a control treatment agent containing tannic acid, metal salt or the tannic acid metal complex prepared in step 1 as an active ingredient is used to coat rice seeds at an active ingredient dosage of 0.1-0.4 g / 100 kg of seeds.

[0045] 3. Rice sowing and cultivation method

[0046] A rice seed treatment agent treatment group with tannic acid, metal salt or tannic acid metal complex prepared in step 1 as the active ingredient and a blank control treatment group were set up, 50 seeds were sown in each treatment, and repeated 3 times. Specifically, nutrient soil was prepared according to peat: vermiculite: chicken manure at a ratio of 7:3:1, tap water was added, and after reaching saturated water content, the coated rice seeds were sown into the soil, the surface was evenly covered with 1 cm thick soil, and placed in a greenhouse for cultivation, and watered once every 3 days. The temperature in the greenhouse was 25-30℃ during the day, 13-15℃ at night, and the air humidity was 50%-90%.

[0047] IV. Survey Methods

[0048] Thirty days after sowing, the number of seedlings in each treatment was investigated, and the seedling rate and the growth rate of the seedling rate were calculated according to the following formula:

[0049]

[0050] V. Test Results

[0051] The rice seed treatment agent with tannic acid, metal salt or tannic acid metal complex prepared in step 1 as an effective ingredient coated rice seeds has an effect on the rice seedling rate as shown in Tables 3 and 4. MnSO4 can increase the rice seed seedling rate by 2.0%-7.3% at a coating dosage of 0.1-0.4 g / 100 kg of seeds. The effects of other metal salts on the seed seedling rate are unstable, and the rice seedlings are inhibited at certain concentrations. Manganese tannate can increase the seedling rate by 6.0%-12.0% at a coating dosage of 0.1-0.4 g / 100 kg of seeds, especially at a seed treatment dosage of 0.3-0.4 g / 100 kg of seeds, which can increase the rice seed seedling rate by 8.7%-12.0%. In addition, zinc tannate can increase the seedling rate by 0.7%-11.3%, especially at a seed treatment dose of 0.3-0.4 g / 100 kg of seeds, which can increase the rice seed seedling rate by 10.0%-13.0%. Manganese tannate and zinc tannate performed best in promoting rice seeding and seedling establishment. Magnesium tannate can increase the seedling rate by 1.3%-7.3% at a coating dose of 0.1-0.3 g / 100 kg of seeds. Other tannic acid metal complexes have unstable effects on rice seedling establishment.

[0052] Table 3. Effects of single treatments of tannic acid and metal salts on rice seedling rate

[0053]

[0054] Table 4. Effects of tannic acid metal complex treatment on rice seedling rate

[0055]

[0056] Example 3: Application of tannic acid metal complex in promoting the growth of rice seedlings

[0057] 1. Preparation of Tannic Acid Metal Complex

[0058] The preparation method of tannic acid metal complex is the same as that in Example 1.

[0059] 2. Rice Seed Treatment

[0060] Preparation of rice seed treatment agent: Referring to the method of Example 1, tannic acid, metal salt and tannic acid metal complex or an equal amount of water are mixed with a film-forming agent and water in a certain proportion to prepare a seed treatment agent or a control treatment agent.

[0061] Seed treatment method: Referring to the method of Example 1, a rice seed treatment agent or a control treatment agent containing tannic acid, metal salt or the tannic acid metal complex prepared in step 1 as an active ingredient is used to coat rice seeds at an active ingredient dosage of 0.1-0.4 g / 100 kg of seeds.

[0062] 3. Rice sowing and cultivation method: Set up rice seed treatment agents with tannic acid, metal salts or tannic acid metal complexes prepared in step 1 as active ingredients and blank control treatment groups, sow 50 seeds for each treatment, and repeat 3 times. Specifically, the sowing and seedling growth conditions are the same as in Example 2.

[0063] IV. Survey method: Survey rice plant height 30 days after sowing, and calculate the growth rate of plant height of each treatment according to the following formula:

[0064]

[0065] V. Test Results

[0066] The effects of rice seed treatment agents with tannic acid, metal salts or tannic acid metal complexes prepared in step 1 as active ingredients on rice seedling height are shown in Tables 5 and 6. Tannic acid alone has a significant growth-promoting effect on rice plant height at a dosage of 0.2-0.4 g / 100 kg seeds, but has an inhibitory effect on seedling growth at a dosage of 0.1 g / 100 kg seeds. Different metal salts have different effects on the growth of rice seedlings. At a dosage of 0.1-0.4 g / 100 kg seeds, MnSO4 and Zn(CH3COO)2 can increase plant height by 24.45%-68.69% and 5.96%-23.73%, respectively, showing a growth-promoting effect. In contrast, manganese tannate and zinc tannate can significantly increase the height of rice plants, reaching 67.17%-81.16% and 15.35%-55.61% respectively. In particular, at a seed treatment dose of 0.3-0.4 g / 100 kg of seeds, the height of rice seedlings can be increased by 69.17%-81.16% and 23.55%-55.61%, which is the best performance. In addition, iron tannate can increase the height of rice plants by 18.86%-38.03% at a dosage of 0.2-0.4 g / 100 kg of seeds. Manganese tannate, zinc tannate and iron tannate all showed the effect of promoting the growth of rice seedlings, while other tannic acid metal complexes showed the effect of inhibiting plant height growth at some dosages, and the effect of promoting the growth of rice seedlings was unstable.

[0067] Table 5. Effects of single treatments of tannic acid and metal salts on rice plant height

[0068]

[0069] Table 6. Effects of single treatments of tannic acid and metal salts on rice plant height

[0070]

[0071] The invention finds that a tannic acid metal complex is prepared by mixing a tannic acid solution with a zinc salt or a manganese salt solution, stirring and reacting for 3 hours, wherein the molar ratio of tannic acid to zinc salt or manganese salt is 1:20-30; the tannic acid metal complex is used for seed treatment at a dosage of 0.1-0.4 g / 100 kg of seeds, and has a good effect on improving the germination rate and seedling rate of rice seeds and promoting the growth of rice seedlings (increasing the height of rice plants).

[0072] The research results of the present invention show that manganese tannate can increase the germination rate of rice seeds by 6.0%-16.0%, the seedling rate by 6.0%-12.0%, and the plant height by 69.17%-81.16% at a seed treatment dosage of 0.1-0.4 g / 100 kg of seeds; zinc tannate can increase the germination rate of rice seeds by 6.0%-13.0%, the seedling rate by 0.7%-11.3%, and the plant height by 15.35%-55.61% at a seed treatment dosage of 0.3-0.4 g / 100 kg of seeds. In particular, the promotion effect on rice germination, seedling formation, and seedling growth is optimal at a seed treatment dosage of 0.3-0.4 g / 100 kg of seeds. Manganese tannate can increase the germination rate of rice seeds by 14.0%-16.0%, the seedling rate by 8.7%-12.0%, and the plant height by 69.17%-81.16%; zinc tannate can increase the germination rate of rice seeds by 10.0%-13.0%, the seedling rate by 4.0%-11.3%, and the plant height by 23.55%-55.61%. Tannic acid is environmentally friendly, and the present invention provides a new use of such compounds, and the tannic acid metal complex can be used as a seed treatment agent for direct seeding of rice seeds.

[0073] Although the present invention has been described in detail above by general description, specific implementation methods and experiments, it is obvious to those skilled in the art that some modifications or improvements can be made to the present invention. Therefore, these modifications or improvements made on the basis of the technical solution of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A use of a tannic acid metal complex as a rice seed treatment agent to improve the germination rate of rice seeds, the rice seedling rate and promote the growth of rice seedlings, characterized in that: The tannic acid metal complex is zinc tannic acid and / or manganese tannic acid; the tannic acid metal complex is prepared by mixing a tannic acid aqueous solution with a zinc salt aqueous solution or a manganese salt aqueous solution, and stirring and reacting for 3 hours; wherein the molar ratio of tannic acid to zinc salt or manganese salt is 1:20-30.

2. The use according to claim 1, characterized in that: The molar ratio of the tannic acid to the zinc salt or the manganese salt is 1:27.

5.

3. The use according to claim 1, characterized in that: The zinc salt is Zn(CH3COO)2, ZnSO4, ZnCl2 or Zn(NO3)2.

4. The use according to claim 1, characterized in that: The manganese salt is MnSO4, MnCl2 or Mn(NO3)2.

5. A method for improving the germination rate of rice seeds, the rice seedling rate or promoting the growth of rice seedlings, which comprises using a tannic acid metal complex as a rice seed treatment agent to treat rice seeds, wherein the seed treatment method comprises seed coating, seed soaking, seed mixing or seed pelleting; the tannic acid metal complex is zinc tannate or manganese tannate; the tannic acid metal complex is prepared by mixing a tannic acid aqueous solution with a zinc salt aqueous solution or a manganese salt aqueous solution, and stirring and reacting for 3 hours; wherein, The molar ratio of tannic acid to zinc salt or manganese salt is 1:20-30; the zinc salt is Zn(CH3COO)2, ZnSO4, ZnCl2 or Zn(NO3)2; the manganese salt is MnSO4, MnCl2 or Mn(NO3)2.

6. The method according to claim 5, characterized in that The molar ratio of the tannic acid to the zinc salt or the manganese salt is 1:27.

5.

7. The method according to claim 6, characterized in that: The dosage of the tannic acid metal complex for rice seed treatment is 0.1-0.4 g / 100 kg of seeds.

8. The method according to claim 7, characterized in that: The dosage of the tannic acid metal complex for rice seed treatment is 0.3-0.4 g / 100 kg of seeds.

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