Contactless sperm intelligent screening instrument

Abstract

The present invention provides a non-contact sperm intelligent screening instrument, which relates to the technical field of sperm screening, and includes a screening box, a crushing component, a diversion component, a liquid container and a microscope; the screening box includes a lower box, a cover and a cone seat; a processing chamber is provided in the middle of the cone seat, and the crushing component is arranged in the cone seat for crushing and decomposing testicular tissue; the liquid container is arranged on the lower box, and the diversion component is located between the liquid container and the crushing component, and the diversion component is used to divert the solution containing sperm after shearing and crushing treatment to the liquid container; the microscope is installed on the cover and is located on the top of the liquid container; an ultraviolet sterilization lamp is installed on the inner wall of the cover, and an air circulation purification fan is arranged in the lower box. The present invention integrates multiple steps of traditional sperm screening into one instrument, which is convenient for operation and observation, and sterile operation is performed throughout the process, which reduces the possibility of sperm contamination and ensures sperm activity.

Description

Technical Field

[0001] The present invention relates to the technical field of sperm screening, and in particular to a contactless sperm intelligent screening instrument. Background Art

[0002] Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technology and a component of an in vitro fertilization cycle. The in vitro selection of ICSI sperm is very important and directly affects the father's information transmission to the pre-implantation embryo. The most commonly used method for selecting sperm for ICSI is to evaluate sperm motility and morphology under a microscope.

[0003] Most existing sperm screening methods use direct observation under a microscope, using the principle of convex lens to magnify the sperm structure and plan the instrument line to judge sperm activity based on the sperm movement distance. The relevant screening instruments have a simple structure and are only used to clearly see the sperm structure and its movement process. The sperm is easily contaminated during the screening operation, making the observed sperm unusable for fertilization. There is a lack of contactless and sterile sperm detection and screening instruments. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a contactless sperm intelligent screening instrument, which integrates multiple steps of traditional sperm screening operations such as shearing and crushing, filtration, liquid separation, and observation and screening into one instrument. Finally, the target sperm can be taken out, which is convenient for operation and observation. The whole process is sterile, which reduces the possibility of sperm contamination and ensures sperm activity.

[0005] In order to achieve the above-mentioned object, the present invention provides a non-contact sperm intelligent screening instrument, including a screening box, a crushing component, a diversion component, a liquid container and a microscope;

[0006] The screening box body comprises a lower box body, a cover body and a conical seat arranged on the top wall of the cover body; a processing chamber is opened in the middle of the conical seat, and the upper and lower ends of the processing chamber pass through the conical seat and the cover body respectively;

[0007] The crushing assembly is arranged in the cone seat and is used for crushing and decomposing the testicular tissue;

[0008] The liquid container is arranged on the lower box body, the diverter assembly is located between the liquid container and the crushing assembly, and the diverter assembly is used to divert the sperm-containing solution after shearing and crushing to the liquid container;

[0009] The microscope is mounted on the cover and located on the top of the liquid container;

[0010] An ultraviolet sterilization lamp is installed on the inner wall of the cover, and an air circulation purification fan is arranged in the lower box.

[0011] Furthermore, the crushing assembly includes a processing rod and a processing mortar adapted for the processing rod, the front end surface of the processing rod close to the processing mortar protrudes outward to form a hemispherical upper shearing sphere, the upper end surface of the processing mortar is concave to form a lower shearing sphere, and the upper shearing sphere and the lower shearing sphere are densely covered with bidirectional barbs.

[0012] Furthermore, the plurality of bidirectional barbs on the upper shearing spherical surface are arranged in a spiral manner in the circumferential direction, and the plurality of bidirectional barbs on the lower shearing spherical surface are arranged in a uniform and dense manner.

[0013] Furthermore, a retaining ring is sleeved on the processing rod, and the processing rod abuts against the top wall of the cone seat through the retaining ring. The position of the retaining ring in the axial direction of the processing rod is adjustable.

[0014] Furthermore, the processing mortar includes a sleeve and a concave hemispherical body, the hemispherical body is located in the sleeve and close to one end of the processing rod, the outer edge of the hemispherical body is fixedly connected to the outer edge of the sleeve and forms a guide slope, and a plurality of liquid inlet holes are opened on the guide slope.

[0015] Furthermore, a filter screen is arranged in the cone seat, the filter screen is located at the bottom of the processing mortar, and the filter screen is used to filter the testicular tissue debris.

[0016] Furthermore, a rotating platform is provided in the lower box body, a driving motor is connected to the bottom of the rotating platform, the motor is fixed in the lower box body, and a plurality of limiting holes for placing liquid containers are provided on the rotating platform, and the plurality of limiting holes are evenly distributed along the circumference of the rotating platform;

[0017] The diversion component includes a receiving plate and a plurality of liquid distribution droppers, the receiving plate is located at the bottom of the crushing component, the liquid distribution droppers correspond one-to-one to the liquid holding vessels on the limiting holes, one end of the liquid distribution droppers is connected to the inner cavity of the receiving plate, and the other end is located at the top of the liquid holding vessel, and each of the liquid distribution droppers is provided with an electromagnetic control valve to control the opening and closing of the liquid distribution droppers.

[0018] Furthermore, the microscope is connected to a computer, and the microscope can transmit the observed pictures to the computer, and analyze and judge using sperm status analysis software on the computer.

[0019] Furthermore, the top wall of the cover body is provided with a take-in / put-out opening, the take-in / put-out opening is located at the top of one of the limiting holes on the rotating platform, a sliding cover capable of closing the take-in / put-out opening is provided at the take-in / put-out opening, a partition groove capable of placing the sliding cover is provided on the inner wall of the take-in / put-out opening, and the partition groove is connected with the take-in / put-out opening;

[0020] A lifting cylinder is installed in the lower box body, and the lifting cylinder is located directly below the loading and unloading port. The piston rod of the lifting cylinder is connected to a suction cup, and the piston rod of the lifting cylinder can drive the liquid container at the corresponding position to move in the vertical direction through the suction cup.

[0021] Furthermore, a central control device is provided on one side of the screening box, and the lifting cylinder and the driving motor are both connected to the central control device.

[0022] Beneficial effects of the present invention:

[0023] The contactless sperm intelligent screening instrument of the present invention can provide a sterile environment, integrate multiple steps of traditional sperm screening operations such as shearing, crushing, filtering, liquid separation, and observation and screening into one instrument, and finally the target sperm can be taken out, which is convenient for operation and observation. The whole process is sterile, which reduces the possibility of sperm contamination and ensures sperm activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0025] Figure 2 It is a partial cross-sectional view of the present invention for reflecting the internal structure of the screening box.

[0026] Figure 3 It is a cross-sectional view of the present invention for reflecting the internal structure of the screening box.

[0027] Figure 4 It is a schematic diagram of the local structure of the crushing component of the present invention.

[0028] Figure 5 It is a schematic structural diagram of the bidirectional barbed thorn in the present invention.

[0029] In the figure: 1. lower box; 11. rotating platform; 111. limit hole; 12. drive motor; 13. air circulation purification fan; 2. cover; 21. take-in and put-out port; 22. observation position; 23. sliding cover; 24. partition; 25. lifting cylinder; 26. suction cup; 3. cone seat; 31. ultraviolet sterilization lamp; 4. crushing component; 41. processing rod; 411. upper shear sphere; 412. two-way barbed hook; 42. retaining ring; 43. processing mortar; 431. sleeve; 432. hemispherical body; 433. lower shear sphere; 434. guide slope; 435. liquid inlet hole; 44. filter; 5. diversion component; 51. receiving plate; 52. liquid dropper; 6. liquid container; 7. microscope; 8. central control device. DETAILED DESCRIPTION

[0030] The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0031] The invention discloses a contactless sperm intelligent screening instrument.

[0032] Reference Figure 1 and Figure 2 A non-contact sperm intelligent screening instrument includes a screening box, a crushing component 4, a diversion component 5, a liquid container 6 and a microscope 7; the screening box includes a lower box 1, a cover 2 and a cone seat 3 arranged on the top wall of the cover 2; a processing cavity is provided in the middle of the cone seat 3, and its upper and lower ends respectively penetrate the cone seat 3 and the cover 2. An observation position 22 is provided on the cover 2, and the microscope 7 is installed at the observation position 22. An observation light source is provided at the corresponding observation position of the lower box 1 for observation imaging of the microscope 7.

[0033] Reference Figure 2 and Figure 3 The crushing component 4 is arranged in the cone seat 3, which is used to crush and decompose the testicular tissue; the liquid container 6 is arranged on the lower box body 1, and the diversion component 5 is located between the liquid container 6 and the crushing component 4, which is used to divert the solution containing sperm after shearing and crushing to the liquid container 6; an ultraviolet sterilization lamp 31 is installed on the inner wall of the cover body 2, and an air circulation purification fan 13 is arranged in the lower shell body, and a multi-layer filter cotton is arranged on the inner side thereof, so as to achieve sterilization and dust removal of the air in the instrument and prevent sperm pollution.

[0034] The microscope 7 is mounted on the cover 2 and is located on the top of the liquid container 6; it is used to observe the sperm motility state, etc. In addition, the microscope 7 is connected to a computer, and the observed pictures can be transmitted to the computer, and the sperm state analysis software in the computer is used to assist in analyzing and judging the sperm motility. It should be noted that the sperm state analysis software involved in this application has existing announcement numbers CN117911916A, CN117409406A, and the invention patents of CN117409406A have disclosed related technologies, which are not the focus of protection of this application, so they will not be repeated.

[0035] When in use, the extracted testicular tissue is placed in the instrument, and the crushing component 4 is used to grind, decompose and filter it. By adding a test reagent (such as 2 ml, G-MOPS containing 5% HSA), the sperm flows into the liquid container 6 through the diversion component 5, and is observed using a microscope 7 and the observed pictures or videos are transmitted to a computer. The instrument integrates multiple steps of traditional sperm screening operations into one instrument, which is convenient for operation and observation, and is sterile throughout the process, reducing the possibility of contamination.

[0036] Reference Figure 3 to Figure 4In this embodiment, the crushing assembly 4 includes a processing rod 41 and a processing mortar 43 adapted to the processing rod 41. The front end surface of the processing rod 41 near the processing mortar 43 protrudes outward to form a hemispherical upper shearing spherical surface 411, and the upper end surface of the processing mortar 43 is concave to form a lower shearing spherical surface 433. The upper shearing spherical surface 411 and the lower shearing spherical surface 433 are densely covered with bidirectional barbs; wherein, the bidirectional barbs of the upper shearing spherical surface 411 are arranged in a spiral in the circumferential direction, and the bidirectional barbs of the lower shearing spherical surface 433 are evenly and densely arranged. The use of a spherical surface for the processing surface can effectively increase the area of ​​the processing surface and ensure the shearing and crushing effect; the bidirectional barbs arranged in a spiral on the upper shearing spherical surface 411 can tear the testicular tissue to the edge or center of the arc surface by rotating the processing rod 41 in different directions, so as to facilitate the tearing of the testicular tissue.

[0037] Refer to the figure Figure 4 A retaining ring 42 is sleeved on the treatment rod 41, and the treatment rod 41 abuts against the top wall of the cone seat 3 through the retaining ring 42. The position of the retaining ring 42 in the axial direction of the treatment rod 41 can be adjusted, and then the gap between the upper shearing spherical surface 411 and the lower shearing spherical surface 433 can be changed, so as to adjust the size of the grinding tissue. In this embodiment, positioning rings can be set on both sides of the retaining ring 42, and the positioning rings are arranged on the treatment rod 41 and threadedly connected with the treatment rod 41, and then the position of the retaining ring 42 is adjusted by rotating the positioning rings on both sides of the retaining ring 42.

[0038] The processing mortar 43 includes a sleeve 431 and a concave hemispherical body 432. The hemispherical body 432 is located in the sleeve 431 and close to one end of the processing rod 41. The outer edge of the hemispherical body 432 is fixedly connected to the outer edge of the sleeve 431 and forms a guide slope 434. The guide slope 434 is provided with a plurality of liquid inlet holes 435. The guide slope 434 is arranged so that sperm can slowly flow down along the guide slope 434 when the flushing liquid is added, and enter the liquid container 6 through the liquid inlet holes 435.

[0039] In this embodiment, a filter screen 44 is also installed in the cone seat 3. The filter screen 44 is located at the bottom of the processing mortar 43. The filter screen 44 is used to filter the testicular tissue debris.

[0040] Reference Figure 3, a rotating platform 11 is arranged in the lower box 1, a driving motor 12 is connected to the bottom of the rotating platform 11, and the motor is fixed in the lower box 1. A plurality of limiting holes 111 are provided on the rotating platform 11 for placing the liquid container 6. In this embodiment, 12 limiting holes 111 are arranged, and the limiting holes 111 are evenly distributed along the circumference of the rotating platform 11. The flow dividing component 5 includes a receiving plate 51 and a plurality of liquid dividing droppers 52. The receiving plate 51 is located at the bottom of the crushing component 4. The liquid dividing droppers 52 correspond to the liquid containers 6 on the limiting holes 111 one by one. One end of the liquid dividing dropper 52 is connected to the inner cavity of the receiving plate 51, and the other end is located at the top of the liquid container 6. An electromagnetic control valve is provided on each liquid dividing dropper 52 to control the opening and closing of the liquid dividing dropper 52. Through the flow dividing component 5, samples can be added to each liquid container 6 at the same time or to a certain liquid container 6 alone to meet different screening operation requirements.

[0041] Reference Figures 1 to 3 A take-in / take-out opening 21 is provided on the top wall of the cover body 2. The take-in / take-out opening 21 is located at the top of one of the limiting holes 111 on the rotating platform 11. A sliding cover 23 that can close the take-in / take-out opening is provided at the take-in / take-out opening 21. A partition groove 24 for placing the sliding cover 23 is provided on the inner wall of the aperture of the take-in / take-out opening 21. The partition groove 24 is connected to the take-in / take-out opening 21. The take-in / take-out opening 21 is opened and closed by sliding the sliding cover 23.

[0042] A lifting cylinder 25 is installed in the lower box body 1, and the lifting cylinder 25 is located directly below the loading and unloading port 21. The piston rod of the lifting cylinder 25 is connected to a suction cup 26, and the piston rod of the lifting cylinder 25 can drive the liquid container 6 at the corresponding position to move in the vertical direction through the suction cup 26.

[0043] A central control device 8 is provided on one side of the screening box, and the lifting cylinder 25 and the drive motor 12 are both connected to the central control device 8. The central control device 8 is provided with corresponding buttons. When the buttons are pressed, the drive motor 12 rotates the corresponding liquid container 6 to below the outlet 21, and the lifting cylinder 25 operates to lift the liquid container 6 to the outside of the outlet 21, so that the liquid container 6 can be taken out, which is easy to operate.

[0044] The working principle of the non-contact sperm intelligent screening instrument of the present invention is as follows: when in use, the extracted testicular tissue is placed in the instrument, and after adjusting the position of the retaining ring 42, the processing rod 41 is inserted into the cone seat 3, and the processing rod 41 is rotated to shear and crush the testicular tissue, and then the test reagent is added, and the sperm flows into the liquid container 6 through the diversion component 5, and then the rotating platform 11 rotates to rotate the sperm in different liquid containers 6 to the observation position 22, and then the microscope 7 is used to observe and transmit the observed pictures or videos to the computer, and the software in the computer analyzes the activity of the sperm; according to the analysis results, the operator can lift and take out the sperm in the required liquid container 6 by pressing the buttons on the central control device 8. The entire instrument integrates multiple steps of the traditional sperm screening operation into one instrument, which is convenient for operation and observation, and the whole process is aseptic, reducing the possibility of contamination.

[0045] Based on the above ideal embodiments of the present invention, the relevant staff can make various changes and modifications without departing from the technical concept of the present invention through the above description. The technical scope of the present invention is not limited to the contents of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A non-contact sperm intelligent screening instrument, characterized in that: It comprises a screening box, a crushing component (4), a flow dividing component (5), a liquid container (6) and a microscope (7); The screening box comprises a lower box (1), a cover (2) and a conical seat (3) arranged on the top wall of the cover (2); a processing chamber is provided in the middle of the conical seat (3), and the upper and lower ends of the processing chamber respectively pass through the conical seat (3) and the cover (2); The crushing component (4) is arranged in the cone seat (3) and is used for crushing and decomposing testicular tissue; The liquid container (6) is arranged on the lower box body (1), the diverter assembly (5) is located between the liquid container (6) and the crushing assembly (4), and the diverter assembly (5) is used to divert the sperm-containing solution after shearing and crushing treatment into the liquid container (6); The microscope (7) is mounted on the cover (2) and is located on the top of the liquid container (6); An ultraviolet sterilization lamp (31) is installed on the inner wall of the cover body (2), and an air circulation purification fan (13) is arranged in the lower box body (1); The crushing assembly (4) comprises a processing rod (41) and a processing mortar (43) adapted to the processing rod (41); the front end surface of the processing rod (41) close to the processing mortar (43) is convex outward to form a hemispherical upper shearing spherical surface (411); the upper end surface of the processing mortar (43) is concave to form a lower shearing spherical surface (433); the upper shearing spherical surface (411) and the lower shearing spherical surface (433) are densely covered with bidirectional barbs; The plurality of bidirectional barbs on the upper shearing spherical surface (411) are arranged in a spiral pattern in the circumferential direction, and the plurality of bidirectional barbs on the lower shearing spherical surface (433) are arranged in a uniform and dense pattern; The processing rod (41) is sleeved with a retaining ring (42), and the processing rod (41) is in contact with the top wall of the cone seat (3) through the retaining ring (42), and the position of the retaining ring (42) in the axial direction of the processing rod (41) is adjustable; A rotating platform (11) is arranged in the lower box (1), a driving motor (12) is connected to the bottom of the rotating platform (11), the motor is fixed in the lower box (1), a plurality of limiting holes (111) for placing liquid containers (6) are provided on the rotating platform (11), and the plurality of limiting holes (111) are evenly distributed along the circumference of the rotating platform (11); The flow distribution component (5) comprises a receiving plate (51) and a plurality of liquid distribution droppers (52). The receiving plate (51) is located at the bottom of the crushing component (4). The liquid distribution droppers (52) correspond one to one with the liquid storage containers (6) on the limiting holes (111). One end of the liquid distribution droppers (52) is connected to the inner cavity of the receiving plate (51), and the other end is located at the top of the liquid storage container (6). Each of the liquid distribution droppers (52) is provided with an electromagnetic control valve for controlling the opening and closing of the liquid distribution droppers (52).

2. A non-contact sperm intelligent screening instrument according to claim 1, characterized in that: The processing mortar (43) comprises a sleeve (431) and a concave hemispherical body (432), wherein the hemispherical body (432) is located inside the sleeve (431) and close to one end of the processing rod (41), and the outer edge of the hemispherical body (432) is fixedly connected to the outer edge of the sleeve (431) and forms a guide slope (434), and a plurality of liquid inlet holes (435) are provided on the guide slope (434).

3. A non-contact sperm intelligent screening instrument according to claim 2, characterized in that: A filter screen (44) is arranged inside the cone seat (3), and the filter screen (44) is located at the bottom of the processing mortar (43). The filter screen (44) is used to filter testicular tissue debris.

4. A non-contact sperm intelligent screening instrument according to claim 3, characterized in that: The microscope (7) is connected to a computer, and the microscope (7) can transmit the observed pictures to the computer, and analyze and judge the sperm status using sperm status analysis software on the computer.

5. A non-contact sperm intelligent screening instrument according to claim 4, characterized in that: The top wall of the cover body (2) is provided with a take-in / take-out opening (21), the take-in / take-out opening (21) is located at the top of one of the limiting holes (111) on the rotating platform (11), a sliding cover (23) capable of closing the take-in / take-out opening (21) is provided at the take-in / take-out opening (21), the inner wall of the take-in / take-out opening (21) is provided with a partition groove (24) capable of placing the sliding cover (23), and the partition groove (24) is communicated with the take-in / take-out opening (21); A lifting cylinder (25) is installed in the lower box (1). The lifting cylinder (25) is located directly below the loading and unloading port (21). The piston rod of the lifting cylinder (25) is connected to a suction cup (26). The piston rod of the lifting cylinder (25) can drive a liquid container (6) at a corresponding position to move in a vertical direction through the suction cup (26).

6. A non-contact sperm intelligent screening instrument according to claim 5, characterized in that: A central control device (8) is provided on one side of the screening box, and the lifting cylinder (25) and the driving motor (12) are both connected to the central control device (8).

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