Application of a small molecule compound in promoting the growth of tomato seedlings

Abstract

The present invention belongs to the field of agricultural technology, and discloses the use of a small molecule compound (4-(2-((4-iodo-2-methylphenyl)amino)-2-oxoethoxy)-3-methylbenzoic acid, denoted as BGE) or a pharmaceutically acceptable salt thereof in promoting plant growth and development or preparing a product promoting plant growth and development. The present invention proposes for the first time the use of the small molecule compound BGE in promoting plant growth. Experiments have shown that during the cultivation of tomato seedlings, spraying the small molecule compound BGE can significantly promote the plant height, stem thickness, dry weight and fresh weight of tomatoes, thereby being beneficial to the growth and development of tomatoes.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and particularly relates to application of a small molecule compound in promoting the growth of tomato seedlings. Background Art

[0002] tomato( Solanum lycopersicum ) is one of the important economic crops widely cultivated around the world, and is widely favored for its rich nutritional value and high economic benefits. However, during the cultivation process of tomatoes, especially in the seedling stage, they are easily affected by factors such as environmental stress, pests and diseases, and nutritional imbalance, resulting in slow growth and poor development, which in turn affects the final yield and fruit quality.

[0003] Plant growth regulators are a class of chemical substances that can significantly affect plant growth and development at extremely low concentrations. They regulate the physiological processes within plants, promote or inhibit physiological activities such as cell division, cell elongation, organ formation and differentiation, and thus affect the overall growth and development of plants. For example, auxin is one of the earliest discovered plant hormones, which can promote the elongation of plant cells, regulate light morphogenesis and phototropism, etc. (Teale, WD, Paponov, IA, & Palme, K. (2006). Auxin in action: signalling, transport and the control of plant growth and development. Nature Reviews Molecular Cell Biology, 7, 847-859). However, although traditional plant growth regulators such as 2,4-D have a good effect in promoting fruit setting of Solanaceae vegetables, they also have some shortcomings in practical applications, such as poor mobility, low absorption efficiency, and causing phytotoxicity, which to a certain extent limit their application (Grossmann, K. (2003). Mediation ofherbicide effects by hormone interactions. Journal of Plant Growth Regulation, 22, 109-122).

[0004] Therefore, the development of new small molecule compounds that are more easily absorbed by plants and safer has become an important direction in the current research on plant growth regulators. Summary of the invention

[0005] The present invention aims to develop a novel growth-promoting chemical substance which can significantly promote the growth of tomatoes.

[0006] The purpose of the first aspect of the present invention is to provide a use of a small molecule compound or a pharmaceutically acceptable salt thereof in promoting plant growth and development or in preparing a product for promoting plant growth and development.

[0007] The second aspect of the present invention aims to provide a method.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is:

[0009] The first aspect of the present invention provides a use of a small molecule compound or a pharmaceutically acceptable salt thereof in promoting plant growth and development or preparing a product that promotes plant growth and development, wherein the structural formula of the small molecule compound is: .

[0010] The name of the small molecule compound is 4-(2-((4-iodo-2-methylphenyl)amino)-2-oxoethoxy)-3-methylbenzoic acid (4-(2-((4-iodo-2-methylphenyl)amino)-2-oxoethoxy)-3-methylbenzoic acid), CBNumber: CB413896898, and the molecular formula is C 17 H 16 INO4, denoted as BioGro-Enhance (abbreviated as BGE).

[0011] In some embodiments of the present invention, the plant is a dicotyledonous plant.

[0012] In some embodiments of the present invention, the dicotyledonous plant is Arabidopsis thaliana, tobacco or tomato.

[0013] In some embodiments of the present invention, the dicotyledonous plant is tomato.

[0014] In some embodiments of the invention, the pharmaceutically acceptable salt refers to a salt of a free acid or base of a compound represented herein that is non-toxic, biologically tolerable or otherwise biologically suitable for administration to a subject. See generally, S.M.Berge et al., "Pharmaceutical Salts," J. Pharm. Sci., 1977, 66, 1-19. Preferred pharmaceutically acceptable salts are those that are pharmacologically effective and suitable for contact with a subject's tissue without excessive toxicity, irritation or allergic reaction. The compounds described herein may have sufficiently acidic groups, sufficiently basic groups, two types of functional groups, or more than one functional group of each type, and therefore react with many inorganic or organic bases and inorganic and organic acids to form pharmaceutically acceptable salts.

[0015] For compounds described herein that contain a basic group (such as an amine), pharmaceutically acceptable salts can be prepared by any suitable method available in the art, for example, with an inorganic acid (such as hydrochloric acid, hydrobromic acid, sulfuric acid, sulfamic acid, nitric acid, boric acid, phosphoric acid, etc.) or with an organic acid (such as acetic acid, phenylacetic acid, propionic acid, stearic acid, lactic acid, ascorbic acid, maleic acid, oxaloacetic acid, isethionic acid, succinic acid, valeric acid, fumaric acid, malonic acid, pyruvic acid, oxalic acid, glycolic acid, salicylic acid, oleic acid, palmitic acid, lauric acid, pyranosidic acid (such as The free base may be treated with any compatible mixture of an acid such as glucuronic acid or galacturonic acid), an α-hydroxy acid such as mandelic acid, citric acid or tartaric acid, an amino acid such as aspartic acid or glutamic acid, an aromatic acid such as benzoic acid, 2-acetoxybenzoic acid, naphthoic acid or cinnamic acid, a sulfonic acid such as lauryl sulfonic acid, p-toluenesulfonic acid, methanesulfonic acid or ethanesulfonic acid, or any other acid and mixtures thereof deemed to be equivalent or acceptable substitutes according to the level of ordinary skill in the art.

[0016] For compounds described herein containing acidic groups (such as carboxylic acid groups), base addition salts can be prepared by any suitable method available in the art, for example, by treating such compounds with a sufficient amount of the desired base (either neat or in a suitable inert solvent). Examples of pharmaceutically acceptable base addition salts include, but are not limited to, lithium, sodium, potassium, calcium, ammonium, zinc or magnesium salts, or other metal salts; organic amino salts, such as alkyl, dialkyl, trialkyl or tetraalkyl ammonium salts.

[0017] In some preferred embodiments of the present invention, the pharmaceutically acceptable salt is selected from disodium salt, camphorsulfonate, sulfate, pyrosulfate, bisulfate, sulfite, bisulfite, phosphate, monohydrogen phosphate, dihydrogen phosphate, metaphosphate, pyrophosphate, chloride, bromide, iodide, acetate, propionate, decanoate, octanoate, acrylate, formate, isobutyrate, hexanoate, heptanoate, oxalate, malonate, succinate, suberate, sebacate, fumarate, maleate, butyne-1,4-dioate, hexyne-1 , any of the following salts: benzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, phthalate, sulfonate, methylsulfonate, propanesulfonate, benzenesulfonate, xylenesulfonate, naphthalene-1-sulfonate, naphthalene-2-sulfonate, phenylacetate, phenylpropionate, phenylbutyrate, citrate, lactate, γ-hydroxybutyrate, glycolate, tartrate and mandelate.

[0018] In some embodiments of the present invention, the promoting plant growth and development includes at least one of promoting an increase in root weight of tomato seedlings, promoting an increase in stem thickness of tomato seedlings, promoting growth of tomato seedlings, and promoting an increase in dry and fresh weight of tomato seedlings.

[0019] In some embodiments of the present invention, the small molecule compound is used at a concentration of 1 to 50 μM.

[0020] In some embodiments of the present invention, the small molecule compound is used at a concentration of 1 to 40 μM.

[0021] In some embodiments of the present invention, the small molecule compound is used at a concentration of 1 to 20 μM.

[0022] In some embodiments of the present invention, the product includes a reagent (such as a plant growth promoter), a kit, or a drug.

[0023] The second aspect of the present invention provides a method, comprising the step of applying (a1) or (a2) during plant growth;

[0024] (a1) a small molecule compound or a pharmaceutically acceptable salt thereof; the structural formula of the small molecule compound is:

[0025] ;

[0026] (a2) Products containing (a1);

[0027] The method is at least one of (b1) to (b4):

[0028] (b1) A method for promoting the increase of root weight of tomato seedlings;

[0029] (b2) a method for promoting the growth of tomato seedlings;

[0030] (b3) A method for promoting the increase of dry and fresh weight of tomato seedlings;

[0031] (b4) A method for promoting the increase of stem thickness of tomato seedlings.

[0032] In some embodiments of the present invention, the product includes a reagent (such as a plant growth promoter), a kit, or a drug.

[0033] In some embodiments of the present invention, the applying comprises spraying or applying to plants or places where plants grow.

[0034] In some embodiments of the present invention, the product further comprises auxiliary materials.

[0035] In some embodiments of the present invention, the auxiliary materials include at least one of fillers, disintegrants, diluents, dispersants, excipients, stabilizers, lubricants, binders, wetting agents, flavoring agents, solubilizers, suspending agents, solvents, sustained-release agents, emulsifiers, absorption enhancers, surfactants, preservatives, pigments, flavors and solvents.

[0036] In some embodiments of the present invention, the filler is selected from starch, sucrose, lactose, mannitol, sorbitol, xylitol, microcrystalline cellulose or glucose; the binder is selected from cellulose derivatives, alginate, starch, water, dextrin, gelatin or polyvinyl pyrrolidone; the disintegrant is selected from microcrystalline cellulose, sodium carboxymethyl starch, cross-linked polyvinyl pyrrolidone, low-substituted hydroxypropyl cellulose or cross-linked sodium carboxymethyl cellulose; the lubricant is selected from stearic acid, polyethylene glycol, calcium carbonate, sodium bicarbonate, micro-powder silica gel, talc or magnesium stearate; the suspending agent is selected from micro-powder silica gel, beeswax, cellulose, solid polyethylene glycol; the wetting agent is selected from glycerol, Tween-80, hydrogenated castor oil or lecithin; the solvent is selected from ethanol , liquid polyethylene glycol, isopropanol, Tween-80, glycerol, propylene glycol or vegetable oil, the vegetable oil is selected from soybean oil, castor oil, peanut oil, blending oil, etc.: the surfactant is selected from sodium dodecylbenzene sulfonate, stearic acid, polyoxyethylene-polyoxypropylene copolymer, fatty acid sorbitan or polysorbate (Tween), etc.: the flavoring agent is selected from aspartame, sucralose, flavor, stevioside, acesulfame potassium, citric acid or saccharin sodium; the preservative is selected from one of sodium benzoate, benzoic acid, sorbic acid, potassium sorbate, sodium diacetate, sodium dehydroacetate, and butyl parahydroxybenzoate; the cosolvent is one or two of ethanol, propanol, Tween 20, Tween 80, sodium dodecyl sulfate, sodium dodecyl sulfonate, and sodium heptane sulfonate.

[0037] In some embodiments of the present invention, during the process of cultivating the plants, it is necessary to provide the tomatoes with the light and temperature required for their growth and development.

[0038] In some embodiments of the present invention, when the tomato seedlings are at the two-leaf and one-heart stage, spraying is performed once at night and once again one week later, for a total of two times.

[0039] The beneficial effects of the present invention are:

[0040] The present invention proposes for the first time the application of the small molecule compound BGE in promoting plant growth. Experiments have shown that during the cultivation of tomato seedlings, spraying the above-mentioned small molecule compound BGE can significantly promote the plant height, stem thickness, dry weight and fresh weight of tomatoes, thereby being beneficial to the growth and development of tomatoes.

[0041] The small molecule compound is used to treat tomato seedlings, which is simple and convenient to operate. It is a highly effective plant growth regulator and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 This is a growth phenotype diagram of tomato seedlings sprayed with different concentrations in Example 2.

[0043] Figure 2This is a statistical bar graph of plant height and stem thickness of tomato seedlings sprayed with different concentrations in Example 2. In the figure, lowercase letters a, b, and c represent significant differences in the values ​​of physiological indicators of tomato seedlings sprayed with different concentrations at the 5% level.

[0044] Figure 3 This is a statistical bar chart of the fresh weight of tomato seedlings sprayed with different concentrations in Example 2. In the figure, lowercase letters a, b, and c represent that the differences between the values ​​of physiological indicators of tomato seedlings sprayed with different concentrations at the 5% level are significant.

[0045] Figure 4 This is a statistical bar chart of the dry weight of tomato seedlings sprayed with different concentrations in Example 2. In the figure, lowercase letters a, b, and c represent that the differences between the values ​​of physiological indicators of tomato seedlings sprayed with different concentrations at the 5% level are significant. DETAILED DESCRIPTION

[0046] The present invention is further described in detail below through specific examples.

[0047] It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention.

[0048] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0049] The features and performance of the present invention are further described in detail below in conjunction with the embodiments.

[0050] Example 1 Cultivation of tomato seedlings

[0051] Tomato seeds were soaked in 50°C warm water for 15 minutes and then placed in a 28°C constant speed shaker (200 rpm / min) for germination for 3 days, with the water changed every 12 hours.

[0052] When the radicle of the seeds was about 1 cm long, they were sown in 72-well trays with a mixture of peat and vermiculite in a ratio of 3:1. The seedlings were cultured in a plant factory with a temperature setting of 21 / 19°C (day / night), a photoperiod of 12 h light / 12 h dark, and a light intensity of 200 μmol m -2 s -1 .

[0053] After the tomato seedlings grow a true leaf, transplant them into a planting pot and water them with an appropriate amount of Hoagland nutrient solution every 3 days.

[0054] Example 2 The promoting effect of the small molecule compound BGE on tomato seedlings

[0055] The structural formula of the small molecule compound involved in this embodiment is: , the name is 4-(2-((4-iodo-2-methylphenyl)amino)-2-oxoethoxy)-3-methylbenzoic acid, CBNumber: CB413896898, the molecular formula is C 17 H 16 INO4, denoted as BioGro-Enhance (abbreviated as BGE).

[0056] This example verifies the effect of the above-mentioned small molecule compounds on the growth of tomato seedlings through experiments. The specific experiments are as follows:

[0057] When the tomato seedlings (variety Condine Red) were cultured (cultured as in Example 1) to the two-leaf and one-heart stage, tomato plants with similar growth status were selected for spraying treatment. The treatment concentrations were set to 0 μM, 1 μM, 5 μM, and 10 μM BGE aqueous solution (ethanol was used as a cosolvent to dissolve the BGE powder and then diluted with tap water to the required concentration). The spraying was performed once a week, with a spraying amount of 30 mL each time, for a total of two times. 20 plants were treated in each treatment. After the two sprayings, the growth status of each group of tomato seedlings was observed, and the results were recorded and statistically analyzed.

[0058] Based on the above spraying amount and BGE concentration and the molecular formula of BGE (relative molecular mass is 425.22, that is, the molar mass of the substance is 425.22g / mol), the mass of BGE applied to each tomato seedling each time is calculated according to the following formula: amount of substance (mol) = mass of substance (g) / molar mass of substance (g / mol).

[0059] Take concentrations of 1μM and 10μM as examples:

[0060] The mass of BGE sprayed per tomato seedling = 30 mL × 1 μM (i.e. 1 μmol / L) × 425.22 g / mol = 12.756 × 10 -6 g=12.756μg, that is, each tomato seedling is sprayed with 12.756μg BGE each time.

[0061] The mass of BGE sprayed per tomato seedling = 30 mL × 10 μM (i.e. 10 μmol / L) × 425.22 g / mol = 127.56 × 10 -6g=127.56μg, that is, each tomato seedling is sprayed with 127.56μg BGE each time.

[0062] The results showed that compared with the blank control group (spraying 0μM BGE aqueous solution, i.e. spraying water), the growth of tomato seedlings after spraying BGE was significantly better. BGE small molecule compounds at concentrations of 1μM, 5μM, and 10μM all significantly promoted tomato growth in a dose-dependent manner ( Figure 1 10μM concentration of BGE had the best effect on promoting the growth of tomato seedlings, increasing tomato plant height by 27.90% and stem diameter by 29.74% ( Figure 2 and Table 1), while the dry weight and fresh weight of the aboveground and underground parts also increased significantly ( Figure 3 , Figure 4 and Table 1).

[0063] Table 1 Plant height, stem diameter, fresh weight and dry weight of tomato seedlings after spraying with different concentrations of BGE

[0064]

[0065] Note: In Table 1, data without the same lowercase letter in the same row indicate significant differences among different treatments ( P <0.05).

[0066] In summary, the small molecule compound BioGro-Enhancer provided by the present invention can significantly increase the biomass of tomatoes, is simple and convenient to operate, is a highly effective plant growth regulator, and has broad application prospects.

[0067] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present invention. In addition, the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

Claims

1. Application of a small molecule compound in promoting tomato growth and development, characterized in that: The structural formula of the small molecule compound is: ; The promoting of tomato growth and development is at least one of promoting an increase in root weight of tomato seedlings, promoting an increase in stem thickness of tomato seedlings, and promoting an increase in dry and fresh weight of tomato seedlings.

2. The use according to claim 1, characterized in that: The small molecule compound is used at a concentration of 1 to 10 μM.

3. A method, characterized in that The method comprises the steps of applying a small molecule compound during the growth of tomato seedlings; The structural formula of the small molecule compound is: ； The method is at least one of (b1) to (b3): (b1) A method for promoting the increase of root weight of tomato seedlings; (b2) a method for promoting the increase of dry and fresh weight of tomato seedlings; (b3) A method for promoting the increase of stem thickness of tomato seedlings.

4. The method according to claim 3, characterized in that The application includes spraying onto tomato seedlings or onto the place where tomato seedlings grow.

5. The method according to claim 4, characterized in that The application amount of the small molecule compound is 12.756-127.56 μg / plant / time.

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