Pseudo-ginseng fibrous root extract as well as preparation method and application thereof

Abstract

The invention provides a panax notoginseng fibrous root extract as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The preparation method comprises the following steps: (1) carrying out steam explosion treatment on panax notoginseng fibrous roots to obtain a steam explosion treatment substance; (2) extracting by using a weakly acidic aqueous solution, and collecting filtrate and filter residues; drying the filtrate to obtain a panax notoginseng fibrous root extract A; (3) mixing the filter residue with a surfactant and an organic solvent, performing ultrasonic treatment after heating extraction, and drying the filtrate to obtain a panax notoginseng fibrous root extract B; the surfactant comprises polyethylene glycol 200 and Tween 80 in a weight ratio of (2-4): 1; the volume concentration of ethanol in the organic solvent is 15-30%, and the volume concentration of ethyl acetate is 50-60%; and (4) mixing the panax notoginseng fibrous root extract A and the panax notoginseng fibrous root extract B to obtain the panax notoginseng fibrous root extract. The panax notoginseng fibrous root extract disclosed by the invention has remarkable anti-oxidation and anti-tumor effects.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a notoginseng root extract and a preparation method and application thereof. Background Art

[0002] Panax notoginseng, also known as Tianqi and Jinbuhuan, tastes sweet and slightly bitter, is warm in nature, and mainly enters the sweet, stomach, and large intestine meridians. It has the effects of nourishing, strengthening, replenishing blood, reducing swelling and relieving pain, stopping bleeding and removing blood stasis, and enhancing immunity. A large number of chemical components have been isolated from Panax notoginseng, mainly Panax notoginseng saponins (PNS), polysaccharides, flavonoids, sterols, volatile oils, amino acids, and various trace elements.

[0003] At present, most of the research on the chemical components of Panax notoginseng is focused on Panax notoginseng saponins. Panax notoginseng total saponins have the effects of promoting blood circulation and removing blood stasis, dredge meridians and activate collaterals, inhibit platelet aggregation and increase cerebral blood flow, and are widely used in drugs for the treatment of diseases such as cerebrovascular sequelae, central retinal vein occlusion, and hyphema. Panax notoginseng total saponins and some of its monomer compounds have good pharmacological activities in the cardiovascular system, nervous system, material metabolism, as well as anti-inflammatory and anti-tumor aspects. Panax notoginseng total saponins and its monomer saponins Rb1 and Rg1 can significantly enhance the learning and memory ability of mice; Panax notoginseng total saponins can also reduce the body's oxygen consumption, improve the body's tolerance to hypoxia, inhibit platelet aggregation, dilate cerebral blood vessels, increase cerebral blood flow, and have anti-thrombotic and anti-coagulant effects.

[0004] Different methods of extracting Panax notoginseng result in different components and contents of saponins, which in turn lead to different effects. Chinese invention patent CN116270779A discloses a method for preparing Panax notoginseng extract, which comprises the steps of extracting Panax notoginseng using an extraction solvent, wherein the extraction solvent comprises, by weight, 10-30 parts of citric acid aqueous solution, 1-12 parts of propylene glycol, and 0.1-5 parts of sodium alginate-fixed xylanase. The preparation method improves the extraction efficiency of the active ingredients in the Panax notoginseng extract by controlling the composition of the extraction solvent, the content of each component in the solvent, and the mass ratio of Panax notoginseng to the extraction solvent. The total saponin content of Panax notoginseng obtained by the method is not less than 84.2%, and the effective ingredients include ginsenoside R1 of not less than 8.1%, ginsenoside Rg1 of not less than 34.6%, ginsenoside Rb1 of not less than 37.2%, and ginsenoside Rd of not less than 5.5%.

[0005] Chinese invention patent CN108938696A discloses a method for extracting total saponins of Panax notoginseng, including: enzymatic treatment, water extraction, macroporous resin adsorption, activated carbon adsorption and cation exchange resin adsorption process. The method adds citric acid antioxidant in the water extraction, which can improve the product quality and yield; using water as a solvent to extract Panax notoginseng can reduce the residual organic solvent in the product. Chinese invention patent CN116098288A discloses a Panax notoginseng oral liquid for lowering blood pressure and its preparation method. The invention uses fresh Panax notoginseng fibrous roots as raw materials, and after ultrafine grinding, extracts Panax notoginseng saponins and other active ingredients, and prepares the extract into Panax notoginseng oral liquid. The main active ingredient is Panax notoginseng saponins, which has obvious advantages in anti-hypoxia, aging, hypoxia tolerance and improving human immunity; compared with using ordinary Panax notoginseng roots (main roots) as raw materials, the invention uses ultrafine grinding to greatly promote the dissolution of active ingredients such as Panax notoginseng saponins.

[0006] In addition to notoginseng saponins, other chemical components in Panax notoginseng also have different active effects. Chinese invention patent CN102961424A discloses a Panax notoginseng polysaccharide extract and its preparation method, preparation and application. The Panax notoginseng polysaccharide extract is obtained by extracting the waste residue after extracting the total saponins of Panax notoginseng as raw materials, through extraction, concentration, refining, drying, crushing and inspection steps. The specific steps are: adding the waste residue after extracting the total saponins of Panax notoginseng to purified water, leaching at 50-60℃ for 4-8h, cyclic leaching 2-6 times, filtering, concentrating, adding ethanol precipitation for 8-24h, filtering, refining, and drying to obtain the target dry product. The Panax notoginseng polysaccharide extract is used to prepare anti-tumor drugs and immunity-enhancing drugs.

[0007] In order to achieve the comprehensive extraction and utilization of the chemical components in Panax notoginseng rootlets, it is necessary to provide a method for efficiently extracting multiple chemical components from Panax notoginseng rootlets, improve the extraction effect of Panax notoginseng rootlet extract, and give full play to the efficacy of Panax notoginseng rootlet extract. Summary of the invention

[0008] The present invention aims at the problems existing in the prior art and provides a Panax notoginseng root extract and a preparation method and application thereof. The Panax notoginseng root is first subjected to steam explosion treatment, and then extracted with a weakly acidic aqueous solution to obtain a Panax notoginseng root extract A rich in water-soluble chemical components; then the filter residue is mixed with a surfactant and an organic solvent, heated and extracted, and then ultrasonically treated to obtain a Panax notoginseng root extract B; the Panax notoginseng root extract A and the Panax notoginseng root extract B are mixed to obtain a Panax notoginseng root extract. The Panax notoginseng root extract of the present invention has significantly improved effects of anti-oxidation and inhibition of cancer cells.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0010] First, the present invention provides a method for preparing a Panax notoginseng root extract, comprising the steps of:

[0011] (1) After the Panax notoginseng fibrous roots are soaked in a weakly acidic aqueous solution, they are subjected to steam explosion treatment at a pressure of 0.5-1.2 MPa for 1-3 minutes to obtain a steam-exploded product;

[0012] (2) mixing the steam explosion treated product with a weakly acidic aqueous solution, leaching, filtering, collecting the filtrate and the filter residue; drying the filtrate to obtain a Panax notoginseng root extract A;

[0013] (3) mixing the filter residue from step (2) with a surfactant and an organic solvent, heating and extracting at 40-55° C. for 3-6 hours; ultrasonically treating for 25-60 minutes, filtering, and drying the filtrate to obtain Panax notoginseng root extract B;

[0014] The surfactant is polyethylene glycol 200 (PEG 200) and Tween 80 in a weight ratio of 2-4:1;

[0015] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 15-30%, and the volume concentration of ethyl acetate is 50-60%;

[0016] (4) Mixing the Panax notoginseng root hair extract A of step (2) and the Panax notoginseng root hair extract B of step (3) to obtain a Panax notoginseng root hair extract.

[0017] Preferably, in step (1) and step (2), the weakly acidic aqueous solution is an aqueous solution of formic acid with a volume concentration of 0.05-0.2%.

[0018] Further preferably, in step (1) and step (2), the weakly acidic aqueous solution is a formic acid aqueous solution with a volume concentration of 0.1%.

[0019] Preferably, in step (1), the soaking time is 8-24 hours; more preferably, the soaking time is 12 hours.

[0020] Preferably, in step (1), the soaking is to completely submerge the Panax notoginseng rootlets in a weakly acidic aqueous solution.

[0021] Preferably, in step (1), the steam explosion treatment condition is 1.0 MPa for 2 min.

[0022] Preferably, in step (2), the leaching is specifically: leaching with stirring at room temperature for 0.5-2h at a solid-to-liquid ratio of 1:20-30g / mL.

[0023] Further preferably, in step (2), the leaching is specifically: leaching with stirring at room temperature for 1 hour at a solid-to-liquid ratio of 1:25 g / mL.

[0024] Preferably, in step (2), the drying is freeze-drying.

[0025] Preferably, in step (3), the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1.

[0026] Preferably, in step (3), the organic solvent is a mixture of ethanol, ethyl acetate and water; wherein the volume concentration of ethanol is 25%, and the volume concentration of ethyl acetate is 55%.

[0027] Preferably, in step (3), the mass ratio of the surfactant to the organic solvent is 2.5-5:100.

[0028] Further preferably, in step (3), the mass ratio of the surfactant to the organic solvent is 3.5:100.

[0029] Preferably, step (3) is specifically as follows: mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:25-35 g / mL, and extracting by heating under reduced pressure at 45-50°C for 4-5 hours; then subjecting the mixture to ultrasonic treatment for 30-50 minutes and filtering, concentrating the filtrate under reduced pressure, removing the organic solvent and drying to obtain Panax notoginseng root extract B.

[0030] Further preferably, step (3) is specifically as follows: mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting by heating at 50°C under reduced pressure for 4 hours; then subjecting the mixture to ultrasonic treatment for 40 minutes and filtering, concentrating the filtrate under reduced pressure, removing the organic solvent and drying to obtain Panax notoginseng root extract B.

[0031] Then, the present invention provides a Panax notoginseng root extract, which is prepared by the above preparation method.

[0032] Finally, the present invention provides the use of the Panax notoginseng root extract in the preparation of drugs with antioxidant and anti-tumor effects.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] 1. The preparation method of the present invention performs steam explosion treatment on the Panax notoginseng rootlets to further destroy the plant tissue of the Panax notoginseng rootlets, which is easy to dissolve the chemically active components efficiently, thereby realizing efficient extraction of the chemically active components from the Panax notoginseng rootlets; weakly acidic water is used for leaching to efficiently extract water-soluble chemically active components.

[0035] 2. The preparation method of the present invention comprises the following steps: subjecting the filter residue after steam explosion and weakly acidic water extraction to organic solvent extraction and assisted ultrasonic treatment, wherein the organic solvent contains Tween 80 and PEG 200, which are beneficial to the dissolution of notoginseng saponins; and the assisted ultrasonic treatment further improves the extraction efficiency of the notoginseng root extract and fully exerts the efficacy of the notoginseng root extract.

[0036] 3. The Panax notoginseng root extract obtained by the preparation method of the present invention has multiple chemically active ingredients that complement and enhance each other, and can be used in drugs with antioxidant and anti-tumor effects, with significantly improved efficacy. DETAILED DESCRIPTION

[0037] The following non-limiting examples can make those of ordinary skill in the art understand the present invention more comprehensively, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention according to the disclosed content, and it should also belong to the scope of the present invention. When the embodiment gives a numerical range, it should be understood that, unless otherwise specified in the present invention, the two endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those of ordinary skill in the technical field to which the present invention belongs.

[0038] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Products from different manufacturers have no significant effect on the effects.

[0039] In the following examples, the polyethylene glycol 200 was purchased from Guangdong Nanhui New Materials Co., Ltd. with the product number of polyethylene glycol PEG200DL; the Tween 80 was purchased from Guangdong Nanhui New Materials Co., Ltd. with the product number of Tween T-80.

[0040] Example 1

[0041] A preparation method of Panax notoginseng root extract is as follows:

[0042] (1) cutting Panax notoginseng root segments (1-2 cm), mixing with a 0.1% formic acid aqueous solution, soaking for 12 hours, and then performing steam explosion treatment at 1.0 MPa for 2 minutes to obtain a steam explosion treated product;

[0043] (2) mixing the steam explosion treated product with a formic acid aqueous solution having a volume concentration of 0.1% at a solid-liquid ratio of 1:25 g / mL, stirring and leaching at room temperature for 1 hour, filtering, collecting the filtrate and the filter residue; concentrating the filtrate, and freeze-drying to obtain Panax notoginseng root extract A;

[0044] (3) mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting by heating at 50° C. under reduced pressure for 4 h; then subjecting the mixture to ultrasonic treatment for 40 min and filtering; concentrating the filtrate under reduced pressure, removing the organic solvent, and then freeze-drying the filtrate to obtain Panax notoginseng root extract B;

[0045] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0046] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0047] (4) Mixing the Panax notoginseng root hair extract A of step (2) and the Panax notoginseng root hair extract B of step (3) to obtain a Panax notoginseng root hair extract.

[0048] Example 2

[0049] A preparation method of Panax notoginseng root extract is as follows:

[0050] (1) cutting Panax notoginseng root segments (1-2 cm) into segments, mixing with a 0.05% formic acid aqueous solution, soaking for 12 hours, and then performing a steam explosion treatment at 0.5 MPa for 3 minutes to obtain a steam explosion treated product;

[0051] (2) mixing the steam explosion treated product with a formic acid aqueous solution having a volume concentration of 0.05% at a solid-liquid ratio of 1:25 g / mL, stirring and leaching at room temperature for 1 hour, filtering, collecting the filtrate and the filter residue; concentrating the filtrate, and freeze-drying to obtain Panax notoginseng root extract A;

[0052] (3) mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting by heating under reduced pressure at 45° C. for 5 h; then subjecting the mixture to ultrasonic treatment for 50 min and filtering; concentrating the filtrate under reduced pressure, removing the organic solvent, and then freeze-drying the filtrate to obtain Panax notoginseng root extract B;

[0053] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 2:1; the mass ratio of the surfactant to the organic solvent is 2.5:100;

[0054] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 15% and the volume concentration of ethyl acetate is 60%;

[0055] (4) Mixing the Panax notoginseng root hair extract A of step (2) and the Panax notoginseng root hair extract B of step (3) to obtain a Panax notoginseng root hair extract.

[0056] Example 3

[0057] A preparation method of Panax notoginseng root extract is as follows:

[0058] (1) cutting Panax notoginseng root segments (1-2 cm), mixing with a 0.2% formic acid aqueous solution, soaking for 12 hours, and then performing steam explosion treatment at 1.2 MPa for 1 minute to obtain a steam explosion treated product;

[0059] (2) mixing the steam explosion treated product with a formic acid aqueous solution having a volume concentration of 0.2% at a solid-liquid ratio of 1:25 g / mL, stirring and leaching at room temperature for 1 hour, filtering, collecting the filtrate and the filter residue; concentrating the filtrate, and freeze-drying to obtain Panax notoginseng root extract A;

[0060] (3) mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting by heating at 50° C. under reduced pressure for 4 h; then subjecting the mixture to ultrasonic treatment for 30 min and filtering; concentrating the filtrate under reduced pressure, removing the organic solvent, and then freeze-drying to obtain Panax notoginseng root extract B;

[0061] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 4:1; the mass ratio of the surfactant to the organic solvent is 5:100;

[0062] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 30% and the volume concentration of ethyl acetate is 50%;

[0063] (4) Mixing the Panax notoginseng root hair extract A of step (2) and the Panax notoginseng root hair extract B of step (3) to obtain a Panax notoginseng root hair extract.

[0064] Example 4

[0065] A preparation method of Panax notoginseng root extract is as follows:

[0066] (1) cutting Panax notoginseng root segments (1-2 cm), mixing with a 0.1% formic acid aqueous solution, soaking for 24 hours, and then performing steam explosion treatment at 1.0 MPa for 2 minutes to obtain a steam explosion treated product;

[0067] (2) mixing the steam explosion treated product with a formic acid aqueous solution having a volume concentration of 0.1% at a solid-liquid ratio of 1:20 g / mL, stirring and leaching at room temperature for 2 h, filtering, collecting the filtrate and the filter residue; concentrating the filtrate, and freeze-drying to obtain Panax notoginseng root extract A;

[0068] (3) mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:35 g / mL, and extracting by heating under reduced pressure at 55° C. for 3 h; then subjecting the mixture to ultrasonic treatment for 25 min and filtering; concentrating the filtrate under reduced pressure, removing the organic solvent, and then freeze-drying to obtain Panax notoginseng root extract B;

[0069] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 5:100;

[0070] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 20% and the volume concentration of ethyl acetate is 55%;

[0071] (4) Mixing the Panax notoginseng root hair extract A of step (2) and the Panax notoginseng root hair extract B of step (3) to obtain a Panax notoginseng root hair extract.

[0072] Example 5

[0073] The difference from Example 1 is that step (3) of the preparation method is different:

[0074] Step (1) to step (2) are the same as in Example 1;

[0075] (3) mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting by heating under reduced pressure at 40° C. for 6 h; then subjecting the mixture to ultrasonic treatment for 60 min and filtering; concentrating the filtrate under reduced pressure, removing the organic solvent, and then freeze-drying the filtrate to obtain Panax notoginseng root extract B;

[0076] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0077] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0078] Step (4) is the same as in Example 1.

[0079] Comparative Example 1

[0080] Different from Example 1, the steam explosion treatment in step (1) and the leaching treatment in step (2) were not performed.

[0081] A method for preparing a Panax notoginseng root extract is as follows:

[0082] The Panax notoginseng root segments (1-2 cm) were mixed with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating at 50°C under reduced pressure for 4 hours; then subjected to ultrasonic treatment for 40 minutes and filtered; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain the Panax notoginseng root extract;

[0083] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0084] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%.

[0085] Comparative Example 2

[0086] The difference from Example 1 is that the surfactant in step (3) is different, and polyethylene glycol 200 is not used in this comparative example.

[0087] Step (1) to step (2) are the same as in Example 1;

[0088] (3) mixing the filter residue of step (2) with Tween 80 and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting by heating at 50° C. under reduced pressure for 4 h; then subjecting the mixture to ultrasonic treatment for 40 min and filtering; concentrating the filtrate under reduced pressure, removing the organic solvent, and then freeze-drying to obtain Panax notoginseng root extract B;

[0089] The mass ratio of Tween 80 to organic solvent is 0.875:100;

[0090] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0091] Step (4) is the same as in Example 1.

[0092] Comparative Example 3

[0093] The difference from Example 1 is that the surfactant in step (3) is different, and Tween 80 is not used in this comparative example.

[0094] Step (1) to step (2) are the same as in Example 1;

[0095] (3) mixing the filter residue of step (2) with polyethylene glycol 200 and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracting by heating at 50° C. under reduced pressure for 4 h; then subjecting the mixture to ultrasonic treatment for 40 min and filtering; concentrating the filtrate under reduced pressure, removing the organic solvent, and then freeze-drying to obtain Panax notoginseng root extract B;

[0096] The mass ratio of polyethylene glycol 200 to the organic solvent is 2.625:100;

[0097] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0098] Step (4) is the same as in Example 1.

[0099] Comparative Example 4

[0100] The difference from Example 1 is that no surfactant is used in step (3). The rest is the same as Example 1.

[0101] Comparative Example 5

[0102] Different from Example 1, the volume concentration of the organic solvent in step (3) is different.

[0103] In this comparative example, the organic solvent in step (3) is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 55% and the volume concentration of ethyl acetate is 25%.

[0104] Comparative Example 6

[0105] The difference from Example 1 is that the organic solvent extraction treatment is performed first, and then the steam explosion treatment is performed.

[0106] A method for preparing a Panax notoginseng root extract is as follows:

[0107] (1) Panax notoginseng root segments (1-2 cm) were cut, mixed with a surfactant and an organic solvent at a solid-liquid ratio of 1:30 g / mL, and extracted by heating at 50°C under reduced pressure for 4 h; then subjected to ultrasonic treatment for 40 min, filtered, and a filtrate and a filter residue were obtained; the filtrate was concentrated under reduced pressure, the organic solvent was removed, and then freeze-dried to obtain Panax notoginseng root extract A;

[0108] The surfactant is a mixture of polyethylene glycol 200 and Tween 80, the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; the mass ratio of the surfactant to the organic solvent is 3.5:100;

[0109] The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 25% and the volume concentration of ethyl acetate is 55%;

[0110] (2) mixing the filter residue with a formic acid aqueous solution having a volume concentration of 0.1%, soaking for 12 hours, and then performing a steam explosion treatment at a temperature of 1.0 MPa for 2 minutes to obtain a steam explosion treated product;

[0111] (3) mixing the steam explosion treated product with a formic acid aqueous solution having a volume concentration of 0.1% at a solid-liquid ratio of 1:25 g / mL, stirring and leaching at room temperature for 1 hour, filtering, and collecting the filtrate; concentrating and freeze-drying the filtrate to obtain Panax notoginseng root extract B;

[0112] (4) Mixing the Panax notoginseng root hair extract A of step (2) and the Panax notoginseng root hair extract B of step (3) to obtain a Panax notoginseng root hair extract.

[0113] Experiment 1 Antioxidant activity detection

[0114] 1. Preparation of DPPH alcohol solution: Accurately weigh 20 mg of DPPH (1,1-diphenyl-2-trinitrophenylhydrazine), dissolve it in anhydrous ethanol and dilute it to a 500 mL brown volumetric flask to obtain a DPPH solution with a mass fraction of 0.004%. Store it away from light for later use.

[0115] 2. Preparation of sample solution: Weigh the Panax notoginseng root extract of each example and comparative example, dissolve it in water, make the volume to 10 mL, dilute it, and prepare it to 1 mg / mL to prepare a sample solution.

[0116] 3. Add 1 mL of sample solution to 1 mL of DPPH alcohol solution; mix well with a homogenizer and protect from light. Measure the absorbance at 517 nm. Repeat the above experiment 3 times and calculate the average value.

[0117] The calculation formula of the scavenging rate of Panax notoginseng root extract on DPPH free radicals is:

[0118] DPPH free radical scavenging rate (%) = 1-[(A i -A j ) / A0]×100, where A0 is the absorbance of 1 mL DPPH alcohol solution + 1 mL water, A i A is the absorbance value of 1mL DPPH alcohol solution + 1mL sample solution. j It is the absorbance value of 1mL sample solution + 1mL water.

[0119] Table 1 shows the DPPH free radical scavenging rate results of the Panax notoginseng root extracts prepared in various examples and comparative examples.

[0120] Table 1

[0121]

[0122]

[0123] In Table 1, the comparative example is compared with Example 1. # P≤0.05, ## P≤0.01.

[0124] As can be seen from Table 1, the Panax notoginseng root extract obtained by the preparation method of the present invention has significant DPPH free radical scavenging activity. The result of the DPPH free radical scavenging rate of the Panax notoginseng root extract obtained by the method of the embodiment is significantly higher than that of the comparative example. Therefore, the use of the preparation method of the present invention can significantly improve the antioxidant activity.

[0125] Experiment 2: Detection of tumor cell proliferation inhibition

[0126] 1. Tumor cell culture: Human lung cancer cells (A549) and human liver cancer cells (HepG2) were cultured in RPMI1640 medium containing 10% fetal bovine serum and 10 μg / mL penicillin-streptomycin, and cultured in a constant temperature and sealed environment at 37°C, 5% carbon dioxide, and saturated humidity. Digested with trypsin-EDTA solution (0.25%:0.02%), and passaged every 2 days.

[0127] 2. Experimental samples: Panax notoginseng root extracts prepared in Examples 1 to 5 and Comparative Examples 1 to 6.

[0128] The MTT method was used to detect the inhibitory effect of Panax notoginseng root extract (0.4 mg / mL) on tumor cell proliferation. The inhibition rate on human lung cancer cells and human liver cancer cells are shown in Table 2.

[0129] Table 2

[0130]

[0131]

[0132] In Table 2, in the same column of data, the comparative example is compared with Example 1. # P≤0.05, ## P≤0.01.

[0133] As can be seen from Table 2, Example 1 has a significantly higher inhibitory effect on tumor cell proliferation than the control group. And compared with the control group, each example group has a significantly improved inhibitory effect on tumor cell proliferation. The Panax notoginseng root extract obtained by the preparation method of the present invention has a significantly improved anti-tumor effect.

[0134] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a Panax notoginseng root extract, characterized in that: Includes steps: (1) After the Panax notoginseng fibrous roots are soaked in a weakly acidic aqueous solution, they are subjected to steam explosion treatment at a pressure of 0.5-1.2 MPa for 1-3 minutes to obtain a steam-exploded product; (2) mixing the steam explosion treated product with a weakly acidic aqueous solution, leaching, filtering, collecting the filtrate and the filter residue; drying the filtrate to obtain a Panax notoginseng root extract A; (3) mixing the filter residue from step (2) with a surfactant and an organic solvent, heating and extracting at 40-55° C. for 3-6 hours; ultrasonically treating for 25-60 minutes, filtering, and drying the filtrate to obtain Panax notoginseng root extract B; The surfactant is polyethylene glycol 200 and Tween 80 in a weight ratio of 2-4:1; The organic solvent is a mixture of ethanol, ethyl acetate and water, wherein the volume concentration of ethanol is 15-30%, and the volume concentration of ethyl acetate is 50-60%; (4) Mixing the Panax notoginseng root hair extract A of step (2) and the Panax notoginseng root hair extract B of step (3) to obtain a Panax notoginseng root hair extract.

2. The preparation method according to claim 1, characterized in that: In step (1) and step (2), the weakly acidic aqueous solution is a formic acid aqueous solution with a volume concentration of 0.05-0.2%.

3. The preparation method according to claim 1, characterized in that: In step (1), the soaking time is 8-24 hours.

4. The preparation method according to claim 1, characterized in that: In step (1), the steam explosion treatment condition is 1.0 MPa for 2 min.

5. The preparation method according to claim 1, characterized in that: In step (2), the leaching is specifically: leaching with stirring at room temperature for 0.5-2h at a solid-liquid ratio of 1:20-30g / mL.

6. The preparation method according to claim 1, characterized in that: In step (3), the weight ratio of polyethylene glycol 200 to Tween 80 is 3:1; The organic solvent is a mixture of ethanol, ethyl acetate and water; wherein the volume concentration of ethanol is 25%, and the volume concentration of ethyl acetate is 55%.

7. The preparation method according to claim 1, characterized in that: In step (3), the mass ratio of the surfactant to the organic solvent is 2.5-5:

100.

8. The preparation method according to claim 1, characterized in that: Step (3) is specifically as follows: mixing the filter residue of step (2) with a surfactant and an organic solvent at a solid-liquid ratio of 1:25-35 g / mL, and extracting by heating under reduced pressure at 45-50°C for 4-5 hours; filtering after ultrasonic treatment for 30-50 minutes, concentrating the filtrate under reduced pressure, removing the organic solvent and drying to obtain Panax notoginseng root extract B.

9. A Panax notoginseng root extract, characterized in that Prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the Panax notoginseng root extract according to claim 9 in the preparation of drugs with antioxidant and anti-tumor effects.

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