Sleep-aiding fermentation composition capable of prolonging slow-wave sleep time and preparation method of sleep-aiding fermentation composition

Abstract

The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a sleep-aiding fermentation composition capable of prolonging slow-wave sleep duration and a preparation method of the sleep-aiding fermentation composition. The fermentation composition is prepared from the following components in parts by weight: 10 to 40 parts of cordyceps sobolifera sporocarp, 20 to 70 parts of sparassis crispa, 150 to 400 parts of gastrodia elata, 8 to 38 parts of enzyme preparation and 0.08 to 0.47 part of leavening agent. The traditional Chinese medicine components have a synergistic effect, the effects of all the components are fully exerted, the obtained fermented composition has the effects of soothing the nerves and helping sleep, the sleep time of the slow-wave sleep stage is mainly prolonged, and better sleep quality can be achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine fermentation, and particularly relates to a sleep-aiding fermentation composition for prolonging slow-wave sleep duration and a preparation method thereof. Background Art

[0002] Sleep is the necessary rest to restore energy and maintain health. It is one of the ways for humans to prolong life. It is an important part of the body's recovery, integration and consolidation of memory. It is an indispensable part of health. Insomnia is a very common sleep disorder in clinical practice. It is not only a separate disease, but also can appear as a comorbidity in many diseases. The clinical manifestations of insomnia are difficulty falling asleep, waking up easily at night, and being unable to fall asleep again after waking up, and sometimes waking up while sleeping. In severe cases, people cannot fall asleep all night, accompanied by headaches, dizziness, fatigue, forgetfulness, etc. If people's lives and work are in a state of insomnia for a long time, their bodies will become worse and worse, and the probability of inducing some other diseases they suffer from will also be greatly increased.

[0003] Human sleep is composed of non-rapid eye movement sleep (NREMS) and rapid eye movement sleep (REMS). Non-rapid eye movement sleep can be divided into four stages from shallow to deep: Stage 1 is the shallow sleep stage, which only lasts a few minutes. It is a transition period from wakefulness to deep sleep and is not real sleep; Stage 2 sleep accounts for about half of the NREMS time. During this stage, brain activity slows down, eye activity stops, and people may have trivial, short and incoherent thinking activities, regular breathing, and lower body temperature; Stage 3 is the moderate sleep period, and Stage 4 begins to enter the deep sleep period. Stages 3 and 4 are collectively referred to as slow-wave sleep (SWS), and there is no qualitative difference between Stages 3 and 4.

[0004] NREMS and REMS are two mutually transforming phases. The sleep of adults first enters the NREMS phase, which lasts for about 90 to 110 minutes, and then enters the REMS phase. The REMS phase lasts for about 10 to 30 minutes, and then enters the NREMS phase. The specific process is to gradually move from stage 1 of the NREMS phase to stage 4, and then from stage 4 to the REMS phase (the first sleep cycle), and then from stage 2 to stage 4 and then to the REMS phase (this is the second sleep cycle). After that, a sleep cycle occurs every 90 minutes or so, and there are about 5 to 6 sleep cycles every night. However, after the second sleep cycle, stages 3 and 4 begin to decrease significantly until they disappear, and the duration of the REMS phase gradually increases. Adults can directly enter the wakefulness state in both the NREMS phase and the REMS phase, that is, they are awakened, but the wakefulness state can only enter the NREMS phase, and cannot directly enter the REMS phase.

[0005] The purpose of treating insomnia is mainly to improve the sleep quality of insomniacs, and the improvement of sleep quality is mainly reflected by increasing the duration of SWS, so the development of products that can selectively increase SWS is the focus of research and development. Summary of the invention

[0006] To this end, the present invention provides a sleep-aiding fermented composition for prolonging the duration of slow-wave sleep. On the one hand, the composition has the effect of calming the nerves and promoting sleep, and on the other hand, it can improve sleep quality and significantly prolong the sleep time of SWS.

[0007] To achieve the above object, the technical solution of the present invention is:

[0008] The invention provides a sleep-aiding fermented composition for prolonging the duration of slow-wave sleep, comprising Cicadae fungi fruiting bodies, Hydrangea serrata and Gastrodia elata.

[0009] Preferably, by weight, the composition comprises 10-40 parts of Cordyceps sinensis fruiting bodies, 20-70 parts of Hydrangea spp., 150-400 parts of Gastrodia elata, 8-38 parts of enzyme preparations and 0.08-0.47 parts of leavening agents.

[0010] Further preferably, based on weight, the composition comprises 20-30 parts of Cordyceps sinensis fruiting bodies, 30-65 parts of Hydrangea spp., 200-300 parts of Gastrodia elata, 14-30 parts of enzyme preparations and 0.14-0.32 parts of leavening agents.

[0011] More preferably, the method comprises, by weight, 25 parts of Cordyceps sinensis fruiting bodies, 50 parts of Hydrangea spp., 240 parts of Gastrodia elata, 20 parts of enzyme preparation and 0.22 parts of leavening agent.

[0012] Preferably, the starter includes Lactobacillus rhamnosus ( Lactobacillus rhamnosus )、Lactobacillus plantarum( Lactobacillus plantarum ), Lactococcus lactis subsp. lactis ( Lactococcus lactis subsp. .milk ) at least one of.

[0013] More preferably, the starter consists of Lactobacillus rhamnosus, Lactobacillus plantarum and Lactococcus lactis subsp. lactis.

[0014] More preferably, the starter comprises, by weight, 0.02-0.1 parts of Lactobacillus rhamnosus, 0.05-0.25 parts of Lactobacillus plantarum, and 0.01-0.12 parts of Lactococcus lactis subsp. lactis.

[0015] Further preferably, the weight ratio of Lactobacillus rhamnosus, Lactobacillus plantarum and Lactococcus lactis subsp. lactis is (3-8):(8-15):(3-9).

[0016] More preferably, the weight ratio of Lactobacillus rhamnosus, Lactobacillus plantarum and Lactococcus lactis subsp. lactis is 2:6:3.

[0017] More preferably, the viable bacteria count of Lactobacillus rhamnosus is 10-100 billion CFU / g, the viable bacteria count of Lactobacillus plantarum is 100-200 billion CFU / g, and the viable bacteria count of Lactococcus lactis subsp. lactis is 80-300 billion CFU / g.

[0018] Preferably, the enzyme preparation includes at least one of cellulase, pectinase and acid protease.

[0019] Further preferably, the enzyme preparation comprises 5-20 parts of cellulase and 3-18 parts of acid protease in parts by weight.

[0020] Further preferably, the weight ratio of the cellulase to the acid protease is 3:2.

[0021] More preferably, the enzymatic activity of the cellulase is 20000-40000 U / g, and the enzymatic activity of the acid protease is 50000-100000 U / g.

[0022] Preferably, it also includes an acid-base regulator, which is selected from one or more of citric acid, sodium hydroxide and sodium bicarbonate.

[0023] The present invention also provides a method for preparing a sleep-aiding fermented composition for prolonging slow-wave sleep duration, comprising the following steps:

[0024] (1) Mixing the fruiting bodies of Cicadae Concolor, Hydrangea, Gastrodia elata and water, and adjusting the pH to obtain a Chinese medicine mixed solution;

[0025] (2) adding an enzyme preparation to the traditional Chinese medicine mixture for enzymatic hydrolysis, adjusting the pH, and obtaining an enzymatic hydrolyzate;

[0026] (3) heating the enzymatic hydrolyzate to inactivate the enzyme and extract the enzymatic hydrolyzate to obtain an enzyme-inactivated extract;

[0027] (4) adding a fermentation agent to the enzyme-killed extract to carry out fermentation to obtain a fermentation liquid;

[0028] (5) The fermentation liquid is separated into solid and liquid to obtain fermentation clear liquid.

[0029] Preferably, in step (1), the pH is adjusted to 4.0-5.0.

[0030] Preferably, the enzymatic hydrolysis temperature in step (2) is 40-65° C., and the enzymatic hydrolysis time is 50-100 min.

[0031] More preferably, in step (2), the pH is adjusted to 5.0-6.0.

[0032] Preferably, the enzyme inactivation extraction temperature in step (3) is 90-110° C., and the enzyme inactivation extraction time is 20-80 min.

[0033] Preferably, in step (4), the fermentation temperature is 20-40° C. and the fermentation time is 32-60 h.

[0034] Preferably, in step (1), the Cordyceps sinensis fruiting bodies, Hydrangea serrata and Gastrodia elata are crushed separately and then passed through a 20-200 mesh sieve to obtain powder.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] 1 The fermented composition for prolonging the duration of slow-wave sleep provided by the present invention uses three raw materials, namely, cicada fungus fruiting bodies, hydrangea and gastrodia elata, as the main ingredients. Among them, the cicada fungus fruiting bodies are artificially cultivated and are a traditional Chinese medicinal material. They are non-addictive to taking, and their various active ingredients have obvious effects on the transportation and transformation metabolism of lipids in the body, lowering blood sugar, and anti-infection. Gastrodia elata is a commonly used Chinese medicinal material with a sweet and flat nature and flavor, belonging to the liver meridian. In November 2023, it was officially included in the catalog of "medicine and food homologous" substances. The "Chinese Pharmacopoeia" records that it has anticonvulsant, analgesic, sedative and other effects. The above three raw materials use gastrodia elata as the main raw material, supplemented by cicada fungus fruiting bodies and hydrangea elata. After enzymatic fermentation, the efficacy of each component is fully exerted. The obtained composition has significant effects of calming the nerves and helping sleep, and improving sleep; and it mainly increases the sleep time in the slow-wave sleep stage, which can achieve better sleep quality.

[0037] 2 The mixed strains of Lactobacillus rhamnosus, Lactobacillus plantarum and Lactococcus lactis subsp. lactis used in the present invention can fully exert the synergistic effect to improve the fermentation efficiency and enhance the tranquilizing and sleep-inducing effect of the composition compared with the use of a single strain.

[0038] 3 The fermented composition for calming nerves and promoting sleep provided by the present invention has simple raw materials, few process steps, and is suitable for industrial production. DETAILED DESCRIPTION

[0040] To make the purpose, technical solutions and advantages of the present invention clearer, the embodiments of the present invention are described in further detail below. Based on the embodiments disclosed in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0041] If no specific experimental steps or conditions are specified in the examples, the experiments can be carried out according to the conventional steps or conditions described in the literature in the field. All reagents without manufacturer specified are conventional reagent products that can be obtained through commercial purchase.

[0042] In the following examples, the source information of some products is as follows:

[0043] Cellulase, pectinase and acid protease were purchased from Shandong Longkote Enzyme Preparation Co., Ltd.; Lactobacillus rhamnosus was purchased from Shandong Zhongke Jiayi Bioengineering Co., Ltd. Lactobacillus rhamnosus JYLR-005; Lactobacillus plantarum was purchased from Shaanxi Mufan Biotechnology Co., Ltd. Lactobacillus plantarum LP-28; Lactococcus lactis subsp. lactis ( Lactococcus lactis subsp.lactis )Lactococcus lactis subsp. lactis JYLL-60 was purchased from Shandong Zhongke Jiayi Bioengineering Co., Ltd.; Bifidobacterium animalis subsp. lactis was purchased from Jiangsu Xinshen'ao Biotechnology Co., Ltd.

[0044] Example 1

[0045] The present embodiment provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep, including 25 parts by weight of Cicadae fungi fruiting bodies, 50 parts by weight of Hydrangea, 240 parts by weight of Gastrodia, 12 parts by weight of cellulase, 8 parts by weight of acid protease, 0.04 parts by weight of Lactobacillus rhamnosus, 0.12 parts by weight of Lactobacillus plantarum, 0.06 parts by weight of Lactococcus lactis subsp. lactis, and 3685 parts by weight of water. The enzymatic activity of the cellulase is 20,000 U / g, the enzymatic activity of the acid protease is 50,000 U / g, the viable bacterial count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable bacterial count of Lactobacillus plantarum is 100 billion CFU / g, and the viable bacterial count of Lactococcus lactis subsp. lactis is 80 billion CFU / g.

[0046] Its preparation method is:

[0047] (1) 25 parts by weight of Cordyceps sinensis fruiting bodies, 50 parts by weight of Hydrangea spp., and 240 parts by weight of Gastrodia elata are crushed and passed through a 200-mesh sieve to obtain a Chinese medicine mixed powder; (2) the Chinese medicine mixed powder and 3685 parts by weight of water are uniformly mixed, and citric acid is added to adjust the pH value to 4.8 to obtain a Chinese medicine mixed solution; (3) 12 parts by weight of cellulase and 8 parts by weight of acid protease are added to the Chinese medicine mixed solution, and enzymolysis is performed at a temperature of 55° C. for 90 minutes, and sodium bicarbonate is added to adjust the pH value to 5.6 to obtain an enzymolysis solution; (4) the enzymolysis solution is heated to 100° C. and the enzyme is inactivated for 40 minutes to obtain an enzyme-inactivated extract; (5) after the enzyme-inactivated extract is cooled to 30° C., 0.04 parts by weight of Lactobacillus rhamnosus, 0.12 parts by weight of Lactobacillus plantarum, and 0.06 parts by weight of Lactococcus lactis subsp. lactis are added, and the mixture is fermented at 30° C. for 42 hours to obtain a fermentation solution; (6) the fermentation solution is separated into solid and liquid to obtain an upper fermentation clear liquid, i.e., a fermentation composition.

[0048] Example 2

[0049] The present embodiment provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep, including 20 parts by weight of Cicadae fungi fruiting bodies, 65 parts by weight of Hydrangea, 200 parts by weight of Gastrodia, 5 parts by weight of cellulase, 18 parts by weight of acid protease, 0.02 parts by weight of Lactobacillus rhamnosus, 0.25 parts by weight of Lactobacillus plantarum, 0.01 parts by weight of Lactococcus lactis subsp. lactis, and 3715 parts by weight of water. The cellulase has an enzymatic activity of 20,000 U / g, an enzymatic activity of 50,000 U / g, a viable bacterial count of Lactobacillus rhamnosus of 10 billion CFU / g, a viable bacterial count of Lactobacillus plantarum of 100 billion CFU / g, and a viable bacterial count of Lactococcus lactis subsp. lactis of 80 billion CFU / g.

[0050] Its preparation method is:

[0051] (1) 20 parts by weight of Cordyceps sinensis fruiting bodies, 65 parts by weight of Hydrangea spp., and 200 parts by weight of Gastrodia elata are crushed and passed through a 200-mesh sieve to obtain a Chinese medicine mixed powder; (2) the Chinese medicine mixed powder and 3715 parts by weight of water are uniformly mixed, and citric acid is added to adjust the pH value to 4.8 to obtain a Chinese medicine mixed solution; (3) 5 parts by weight of cellulase and 18 parts by weight of acid protease are added to the Chinese medicine mixed solution, and enzymolysis is performed at a temperature of 40°C for 100 minutes, and sodium bicarbonate is added to adjust the pH value to 5.6 to obtain an enzymolysis solution; (4) the enzymolysis solution is heated to 110°C and the enzyme is inactivated for 20 minutes to obtain an enzyme-inactivated extract; (5) after the enzyme-inactivated extract is cooled to 20°C, 0.02 parts by weight of Lactobacillus rhamnosus, 0.25 parts by weight of Lactobacillus plantarum, and 0.01 parts by weight of Lactococcus lactis subsp. lactis are added, and the mixture is fermented at 20°C for 50 hours to obtain a fermentation solution; (6) the fermentation solution is separated into solid and liquid to obtain an upper fermentation clear liquid, i.e., a fermentation composition.

[0052] Example 3

[0053] The present embodiment provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep, including 10 parts by weight of Cicadae fungi fruiting bodies, 20 parts by weight of Hydrangea, 400 parts by weight of Gastrodia, 20 parts by weight of cellulase, 3 parts by weight of acid protease, 0.1 parts by weight of Lactobacillus rhamnosus, 0.05 parts by weight of Lactobacillus plantarum, 0.12 parts by weight of Lactococcus lactis subsp. lactis, and 3570 parts by weight of water. The cellulase activity is 20,000 U / g, the acid protease activity is 50,000 U / g, the viable bacteria count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable bacteria count of Lactobacillus plantarum is 100 billion CFU / g, and the viable bacteria count of Lactococcus lactis subsp. lactis is 80 billion CFU / g.

[0054] Its preparation method is:

[0055] (1) 10 parts by weight of Cordyceps sinensis fruiting bodies, 20 parts by weight of Hydrangea spp., and 400 parts by weight of Gastrodia elata are crushed and passed through a 200-mesh sieve to obtain a Chinese medicine mixed powder; (2) the Chinese medicine mixed powder and 3570 parts by weight of water are mixed evenly, and citric acid is added to adjust the pH value to 4.8 to obtain a Chinese medicine mixed solution; (3) 20 parts by weight of cellulase and 3 parts by weight of acid protease are added to the Chinese medicine mixed solution, and enzymolysis is performed at a temperature of 65°C for 50 minutes, and sodium bicarbonate is added to adjust the pH value to 5.2 to obtain an enzymolysis solution; (4) the enzymolysis solution is heated to 90°C and the enzyme is inactivated for 80 minutes to obtain an enzyme-inactivated extract; (5) after the enzyme-inactivated extract is cooled to 40°C, 0.1 parts by weight of Lactobacillus rhamnosus, 0.05 parts by weight of Lactobacillus plantarum, and 0.12 parts by weight of Lactococcus lactis subsp. lactis are added, and the mixture is fermented at 40°C for 60 hours to obtain a fermentation solution; (6) the fermentation solution is separated into solid and liquid to obtain an upper fermentation clear liquid, i.e., a fermentation composition.

[0056] Example 4

[0057] The present embodiment provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep, including 40 parts by weight of Cicadae fungi fruiting bodies, 70 parts by weight of Hydrangea, 150 parts by weight of Gastrodia, 16 parts by weight of cellulase, 6 parts by weight of acid protease, 0.08 parts by weight of Lactobacillus rhamnosus, 0.08 parts by weight of Lactobacillus plantarum, 0.09 parts by weight of Lactococcus lactis subsp. lactis, and 3740 parts by weight of water. The enzymatic activity of the cellulase is 20,000 U / g, the enzymatic activity of the acid protease is 50,000 U / g, the viable bacterial count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable bacterial count of Lactobacillus plantarum is 100 billion CFU / g, and the viable bacterial count of Lactococcus lactis subsp. lactis is 80 billion CFU / g.

[0058] Its preparation method is:

[0059] (1) 40 parts by weight of Cordyceps sinensis fruiting bodies, 70 parts by weight of Hydrangea spp., and 150 parts by weight of Gastrodia elata are crushed and passed through a 200-mesh sieve to obtain a Chinese medicine mixed powder; (2) the Chinese medicine mixed powder and 3740 parts by weight of water are uniformly mixed, and citric acid is added to adjust the pH value to 4.8 to obtain a Chinese medicine mixed solution; (3) 16 parts by weight of cellulase and 6 parts by weight of acid protease are added to the Chinese medicine mixed solution, and enzymolysis is performed at a temperature of 60°C for 80 minutes, and sodium bicarbonate is added to adjust the pH value to 5.2 to obtain an enzymolysis solution; (4) the enzymolysis solution is heated to 105°C, and the enzyme is inactivated for 60 minutes to obtain an enzyme-inactivated extract; (5) after the enzyme-inactivated extract is cooled to 35°C, 0.08 parts by weight of Lactobacillus rhamnosus, 0.08 parts by weight of Lactobacillus plantarum, and 0.09 parts by weight of Lactococcus lactis subsp. lactis are added, and the mixture is fermented at 35°C for 32 hours to obtain a fermentation solution; (6) the fermentation solution is separated into solid and liquid to obtain an upper fermentation clear liquid, i.e., a fermentation composition.

[0060] Example 5

[0061] The present embodiment provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep, including 30 parts by weight of Cicadae fungi fruiting bodies, 20 parts by weight of Hydrangea, 300 parts by weight of Gastrodia, 8 parts by weight of cellulase, 14 parts by weight of acid protease, 0.03 parts by weight of Lactobacillus rhamnosus, 0.15 parts by weight of Lactobacillus plantarum, 0.03 parts by weight of Lactococcus lactis subsp. lactis, and 3650 parts by weight of water. The cellulase activity is 20,000 U / g, the acid protease activity is 50,000 U / g, the viable bacteria count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable bacteria count of Lactobacillus plantarum is 100 billion CFU / g, and the viable bacteria count of Lactococcus lactis subsp. lactis is 80 billion CFU / g.

[0062] Its preparation method is:

[0063] (1) 30 parts by weight of Cordyceps sinensis fruiting bodies, 20 parts by weight of Hydrangea spp., and 300 parts by weight of Gastrodia elata are crushed and passed through a 200-mesh sieve to obtain a Chinese medicine mixed powder; (2) the Chinese medicine mixed powder and 3650 parts by weight of water are uniformly mixed, and citric acid is added to adjust the pH value to 4.8 to obtain a Chinese medicine mixed solution; (3) 8 parts by weight of cellulase and 14 parts by weight of acid protease are added to the Chinese medicine mixed solution, and enzymolysis is performed at a temperature of 50°C for 70 minutes, and sodium bicarbonate is added to adjust the pH value to 5.2 to obtain an enzymolysis solution; (4) the enzymolysis solution is heated to 95°C and the enzyme is inactivated for 70 minutes to obtain an enzyme-inactivated extract; (5) after the enzyme-inactivated extract is cooled to 25°C, 0.03 parts by weight of Lactobacillus rhamnosus, 0.15 parts by weight of Lactobacillus plantarum, and 0.03 parts by weight of Lactococcus lactis subsp. lactis are added, and fermentation is performed at 25°C for 45 hours to obtain a fermentation solution; (6) the fermentation solution is separated into solid and liquid to obtain an upper fermentation clear liquid, i.e., a fermentation composition.

[0064] Comparative Example 1

[0065] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep. The only difference between it and Example 1 is that it does not include the fruiting body of Cordyceps sinensis, that is, it includes 50 weight parts of Hydrangea, 240 weight parts of Gastrodia, 12 weight parts of cellulase, 8 weight parts of acid protease, 0.04 weight parts of Lactobacillus rhamnosus, 0.12 weight parts of Lactobacillus plantarum, 0.06 weight parts of Lactococcus lactis subsp. lactis and 3685 weight parts of water. The enzymatic activity of cellulase is 20000U / g, the enzymatic activity of acid protease is 50000U / g, the viable count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable count of Lactobacillus plantarum is 100 billion CFU / g, and the viable count of Lactococcus lactis subsp. lactis is 80 billion CFU / g. However, the total weight of Hydrangea and Gastrodia is the same as the total weight of the fruiting body of Cordyceps sinensis, Hydrangea and Gastrodia in Example 1.

[0066] The preparation method is the same as that in Example 1.

[0067] Comparative Example 2

[0068] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep. The difference between the composition and Example 1 is that the Chinese medicine composition only includes 315 parts by weight of Gastrodia elata, that is, 315 parts by weight of Gastrodia elata, 12 parts by weight of cellulase, 8 parts by weight of acid protease, 0.04 parts by weight of Lactobacillus rhamnosus, 0.12 parts by weight of Lactobacillus plantarum, 0.06 parts by weight of Lactococcus lactis subsp. lactis, and 3685 parts by weight of water. The cellulase activity is 20000U / g, the acid protease activity is 50000U / g, the viable bacteria count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable bacteria count of Lactobacillus plantarum is 100 billion CFU / g, and the viable bacteria count of Lactococcus lactis subsp. lactis is 80 billion CFU / g.

[0069] The preparation method is the same as that in Example 1.

[0070] Comparative Example 3

[0071] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep. The difference between it and Example 1 is that the Chinese medicine does not include hydrangea, that is, it includes 25 weight parts of Cicadae fruiting bodies, 240 weight parts of Gastrodia, 12 weight parts of cellulase, 8 weight parts of acid protease, 0.04 weight part of Lactobacillus rhamnosus, 0.12 weight part of Lactobacillus plantarum, 0.06 weight part of Lactococcus lactis subsp. lactis and 3685 weight parts of water. Wherein the enzyme activity of cellulase is 20000U / g, the enzyme activity of acid protease is 50000U / g, the viable count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable count of Lactobacillus plantarum is 100 billion CFU / g, and the viable count of Lactococcus lactis subsp. lactis is 80 billion CFU / g. However, the total weight of Cicadae fruiting bodies and Gastrodia is the same as the total weight of Cicadae fruiting bodies, hydrangea and Gastrodia in Example 1.

[0072] The preparation method is the same as that in Example 1.

[0073] Comparative Example 4

[0074] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep. The difference between it and Example 1 is that the Chinese medicine does not include Gastrodia elata, that is, it includes 25 weight parts of Cicadae confusa fruiting bodies, 50 weight parts of Hydrangea spp., 12 weight parts of cellulase, 8 weight parts of acid protease, 0.04 weight parts of Lactobacillus rhamnosus, 0.12 weight parts of Lactobacillus plantarum, 0.06 weight parts of Lactococcus lactis subsp. lactis and 3685 weight parts of water. Wherein the enzyme activity of cellulase is 20000U / g, the enzyme activity of acid protease is 50000U / g, the viable bacteria count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable bacteria count of Lactobacillus plantarum is 100 billion CFU / g, and the viable bacteria count of Lactococcus lactis subsp. lactis is 80 billion CFU / g. However, the total weight of Cicadae confusa fruiting bodies and Hydrangea spp. is the same as the total weight of Cicadae confusa fruiting bodies, Hydrangea spp. and Gastrodia elata in Example 1.

[0075] The preparation method is the same as that in Example 1.

[0076] Comparative Example 5

[0077] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep. The difference between it and Example 1 is that the fruiting body of Cicadae is replaced with Pleurotus eryngii, that is, including 25 parts by weight of Pleurotus eryngii, 50 parts by weight of Hydrangea, 240 parts by weight of Gastrodia, 12 parts by weight of cellulase, 8 parts by weight of acid protease, 0.04 parts by weight of Lactobacillus rhamnosus, 0.12 parts by weight of Lactobacillus plantarum, 0.06 parts by weight of Lactococcus lactis subsp. lactis and 3685 parts by weight of water. The enzymatic activity of cellulase is 20000U / g, the enzymatic activity of acid protease is 50000U / g, the viable count of Lactobacillus rhamnosus is 10 billion CFU / g, the viable count of Lactobacillus plantarum is 100 billion CFU / g, and the viable count of Lactococcus lactis subsp. lactis is 80 billion CFU / g. The preparation method is the same as Example 1.

[0078] Comparative Example 6

[0079] This comparative example provides a fermented composition for prolonging slow-wave sleep and promoting tranquility and sleep, which is different from Example 1 only in that the strain contains only 0.22 parts by weight of Lactobacillus rhamnosus. The remaining components and preparation method are the same as those in Example 1. The number of viable bacteria of Lactobacillus rhamnosus is 10 billion CFU / g.

[0080] Comparative Example 7

[0081] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and promoting sleep, which is different from Example 1 only in that the strain contains only 0.22 parts by weight of Lactobacillus plantarum. The remaining components and preparation method are the same as those of Example 1. The number of viable bacteria of Lactobacillus plantarum is 100 billion CFU / g.

[0082] Comparative Example 8

[0083] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and promoting sleep, which is different from Example 1 only in that the strain contains only 0.22 parts by weight of Lactococcus lactis subsp. lactis. The remaining components and preparation method are the same as those in Example 1. The number of viable bacteria of Lactococcus lactis subsp. lactis is 80 billion CFU / g.

[0084] Comparative Example 9

[0085] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and helping sleep, which is different from Example 1 only in that the strain only contains 0.04 weight parts of Lactobacillus rhamnosus, 0.12 weight parts of Lactobacillus plantarum, and 0.06 weight parts of Bifidobacterium animalis subsp. lactis. The remaining components and preparation methods are the same as those in Example 1. The number of viable bacteria of Lactobacillus rhamnosus is 10 billion CFU / g, the number of viable bacteria of Lactobacillus plantarum is 100 billion CFU / g, and the number of viable bacteria of Bifidobacterium animalis subsp. lactis is 100 billion CFU / g.

[0086] Comparative Example 10

[0087] This comparative example provides a fermented composition for prolonging slow-wave sleep and calming the nerves and promoting sleep, which is different from Example 1 only in that the enzyme preparation contains 12 parts by weight of cellulase and 8 parts by weight of pectinase. The preparation method is the same as that of Example 1. The enzymatic activity of the cellulase is 20,000 U / g, and the enzymatic activity of the pectinase is 30,000 U / g.

[0088] Experimental Example 1 Animal Experiment

[0089] In order to verify the effect of the fermented composition of the present invention on calming nerves and promoting sleep, the animal experiment is described below;

[0090] Animals: 6-7 week old male ICR mice, SPF grade, weighing 18-22 g.

[0091] The mice were fed adaptively for one week in an SPF animal laboratory with a breeding temperature of 24±2℃, a relative humidity of 50%~60%, and light and dark alternation for 12h. The mice were randomly divided into 4 groups, with 160 mice in each group. Group 1 conducted an experiment to extend the sleep time of sodium pentobarbital; Group 2 conducted an experiment on the sleep latency of sodium pentobarbital; Group 3 conducted a hypnotic experiment of subthreshold dose of sodium pentobarbital; and Group 4 conducted a direct sleep experiment. Each group was further divided into a normal group, Example 1-5 groups, and Comparative Example 1-10 groups, with 10 mice in each group. The dosing regimen was gavage once a day at a fixed time, according to 200mL per day for the human body, and the gavage dose of mice was calculated by the equivalent dose method of body surface area ratio conversion, which was 26mL / kg. According to this dose, the corresponding fermentation composition was gavaged in Example 1-5 groups and Comparative Example 1-10 groups, and the normal group was gavaged with physiological saline.

[0092] 1.1 Experiment on prolonging sleep time with sodium pentobarbital

[0093] Each group of mice was given the drug by gavage for 14 consecutive days. 15 minutes after the last administration, each group of mice was intraperitoneally injected with sodium pentobarbital solution (38 mg / kg), and the injection volume was 0.1 mL / 10 g according to the weight of the mice. Sleep was measured by the disappearance of the righting reflex of the mice. When the mice were in dorsal recumbency, those that could not right themselves for more than 1 minute were considered to have entered a sleeping state. The sleep duration of each group of mice was recorded.

[0094] 1.2 Pentobarbital sodium sleep latency experiment

[0095] Each group of mice was given the drug by gavage for 14 consecutive days. Fifteen minutes after the last administration, each group of mice was intraperitoneally injected with sodium barbital solution (240 mg / kg). The injection volume was 0.1 mL / 10 g according to the weight of the mice. The disappearance of the righting reflex of the mice was used as an indicator of sleep. When the mice were in dorsal recumbency, those that could not right themselves for more than 1 minute were considered to have entered a sleeping state. The sleep latency of each group of mice was recorded.

[0096] 1.3 Subthreshold dose hypnosis experiment of sodium pentobarbital

[0097] Each group of mice was given the drug by gavage for 14 consecutive days. 15 minutes after the last administration, each group of mice was intraperitoneally injected with sodium pentobarbital solution (26 mg / kg), and the injection volume was 0.1 mL / 10 g according to the weight of the mice. Sleep was measured by the disappearance of the righting reflex of the mice. When the mice were in a dorsal recumbent position, those that could not right themselves for more than 1 minute were considered to have entered a sleeping state. The number of animals that fell asleep in each group within 30 minutes was recorded, and the incidence of sleep was calculated.

[0098] 1.4 Direct sleep test

[0099] Each group of mice was continuously gavaged and administered for 14 days. One hour after the last administration, the mice were observed to see if they entered a sleep state. The mice were judged to enter sleep if their righting reflex disappeared for more than 60 seconds. The direct hypnotic effects on mice in the normal group, Example 1-5 groups and Comparative Example 1-10 groups were compared.

[0100] Table 1 Detection results of sleep indexes of mice in each experimental group

[0101]

[0102] Note: Different letters in the same column represent significant differences. p ≤0.05

[0103] It can be seen from the results in Table 1 that the fermentation composition prepared by the present invention has no direct sleep effect.

[0104] Compared with the normal group, the fermented compositions of Example 1-5 groups can significantly prolong sleep time, shorten sleep latency, and increase the incidence of sleep. According to the results of the "Technical Specifications for Inspection and Evaluation of Health Food 2023" that help sleep, it can be seen that the fermented compositions provided by the present invention have good calming effects on the nerves and helping sleep.

[0105] Compared with Example 1, the fermented compositions of Comparative Examples 1-5 shortened the sleep time, prolonged the sleep latency, and reduced the sleep incidence, indicating that the choice of raw materials has an impact on the effect of improving sleep, and the combination of Cicadae Fungi fruiting bodies, Hydrangea and Gastrodia elata selected in the present invention can achieve a good effect of calming the nerves and promoting sleep.

[0106] Compared with Example 1, the fermentation compositions of Comparative Examples 6-10 have different degrees of reduction in tranquilizing and inducing sleep effects, indicating that the selection of enzyme preparations and fermentation agents also has an impact on improving sleep, and the combination of fermentation agents and enzyme preparations selected in the present invention can well exert tranquilizing and inducing sleep effects.

[0107] In summary, the fermentation composition prepared by the present invention prolongs the sleep time, shortens the sleep latency, increases the sleep incidence, and has no direct sleep effect, indicating that the fermentation composition of the present invention has a good effect of calming the nerves and promoting sleep.

[0108] Experimental Example 2 Sleep Phase Monitoring

[0109] Animals: 6-7 week old male SD rats, SPF grade, weighing 200-220 g.

[0110] Preparation of p-chlorophenylalanine (PCPA) suspension: Weigh PCPA powder and add it to weak alkaline saline (pH 7-8) for shaking, add appropriate amount of Tween 80 to promote dissolution, and ultrasonicate for 30 minutes to prepare a 0.01 mg / mL PCPA suspension.

[0111] Modeling: The mice were placed in an SPF animal laboratory with a temperature of 24±2℃, a relative humidity of 50%~60%, and a light-dark cycle of 12h for one week for adaptive feeding. The rats in the modeling group were intraperitoneally injected with PCPA suspension (concentration of 0.01mg / mL, dose of 0.1mg / kg) every morning to replicate the insomnia rat model for 2 consecutive days. After the second injection of PCPA, the general condition of the rats in the modeling group was observed, and their sleep latency and sleep duration were recorded. If the sleep duration was significantly shortened and the sleep latency was significantly prolonged, it indicated that the insomnia rat model was successfully established.

[0112] Grouping: 10 rats without modeling were randomly selected as the normal group, and the rats with successful modeling were randomly divided into 16 groups, namely the model group, Example 1-5 groups and Comparative Example 1-10 groups.

[0113] Sleep phase monitoring: Rats were first anesthetized with 5% chloral hydrate intraperitoneally. The hair on the head of the anesthetized rats was trimmed and disinfected with alcohol cotton. The head skin of the rats was cut open with surgical scissors at the midline of the eyes and ears, the skull was fully exposed, the periosteum was peeled off, and the surface was wiped with iodine-containing cotton balls until it was dry and clean. The rats were fixed with a stereotaxic instrument, and holes were drilled with a handheld electric drill at the locations on the skull (AP: -5.2mm, ML: +4.5 mm, DV: -3.8mm). Two stainless steel screws with a diameter of 1mm were buried in the skull to make contact with the dura mater but not penetrate the dura mater. The top of the stainless steel screws was firmly wrapped with a wire, and the two buried stainless steel screws were used as two cortical electrodes for recording brain waves. A ground electrode was buried in the middle of the frontal bone slightly to the right. The three electrode screws were made into micro sockets and fixed with dental cement. Penicillin sodium was injected and the skin was sutured. After surgery, the rats were placed in a mouse box and raised in a single cage, and 40,000 units of sodium penicillin for injection were injected intraperitoneally for 3 consecutive days. After 7 days of recovery, each group of rats was gavaged at the same time every day. According to 200 mL per day for the human body, the gavage dose of the rats was calculated by the equivalent dose conversion method of body surface area ratio, which was 18 mL / kg. This dose was gavaged for 7 consecutive days, wherein the corresponding fermentation composition was gavaged in the Example 1-5 group and the Comparative Example 1-10 group, and the normal group and the model group were gavaged with physiological saline. 30 minutes after the sample was administered on the 7th day, the plug with a wire was connected to the microelectrode socket on the head of the rat, and the brain waves of the rat were recorded within 8 hours, and the duration of the slow wave sleep stage 1 (SWS1), slow wave sleep stage 2 (SWS2), rapid eye movement sleep (REMS) and total sleep duration (TST=SWS1+SWS2+REMS) of the rats during this period were calculated.

[0114] Table 2 Sleeping time of rats in each group

[0115]

[0116] Note: Different letters in the same column represent significant differences. p ≤0.05

[0117] From the results in Table 2, it can be seen that compared with the normal group, the SWS1, SWS2 and REMS times of the rats in the model group were significantly shortened; compared with the model group, the SWS1, SWS2 and REMS times of the rats in Examples 1-5 were significantly prolonged, indicating that the fermentation composition prepared in Examples 1-5 can well improve the sleep condition of rats.

[0118] The SWS1, SWS2 and REMS times of the fermented compositions prepared in Comparative Examples 1-5 were significantly lower than those in Example 1, indicating that the combination of Cicadae Convolvulus fruiting bodies, Hydrangea serrata and Gastrodia elata can better improve the sleep condition of rats by working together.

[0119] The SWS1, SWS2 and REMS of the fermentation compositions prepared in Comparative Examples 6-10 are lower than those in Example 1, which indicates that the combination of the fermentation agent and the enzyme preparation selected in the present invention is the optimal combination, and the raw materials can better improve the sleep condition of rats after being treated with the optimal enzyme preparation and fermentation agent.

[0120] In summary, the traditional Chinese medicine composition selected by the present invention, after enzymatic hydrolysis and fermentation, cooperates synergistically, has the effect of calming the nerves and promoting sleep, and mainly increases the sleep time in the slow-wave sleep stage, which can achieve a better effect of improving sleep.

[0121] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. The present invention provides a fermented sleep-aiding composition for prolonging the duration of slow-wave sleep, characterized in that: The invention comprises the following components by weight: 10-40 parts of Cordyceps sinensis fruiting bodies, 20-70 parts of Hydrangea spp., 150-400 parts of Gastrodia elata, 8-38 parts of enzyme preparations and 0.08-0.47 parts of leavening agents.

2. The fermentation composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 20-30 parts of Cordyceps sinensis fruiting bodies, 30-65 parts of Hydrangea spp., 200-300 parts of Gastrodia elata, 14-30 parts of enzyme preparations and 0.14-0.32 parts of leavening agents.

3. The fermentation composition according to claim 1, characterized in that The starter comprises at least one of Lactobacillus rhamnosus, Lactobacillus plantarum and Lactococcus lactis subsp. lactis.

4. The fermentation composition according to claim 3, characterized in that The starter comprises, by weight, 0.02-0.1 parts of Lactobacillus rhamnosus, 0.05-0.25 parts of Lactobacillus plantarum and 0.01-0.12 parts of Lactococcus lactis subsp. lactis.

5. The fermentation composition according to claim 4, characterized in that The weight ratio of Lactobacillus rhamnosus, Lactobacillus plantarum and Lactococcus lactis subsp. lactis is (3-8): (8-15): (3-9).

6. The fermentation composition according to claim 1, characterized in that The enzyme preparation comprises at least one of cellulase, pectinase and acid protease.

7. The fermentation composition according to claim 6, characterized in that The enzyme preparation comprises 5-20 parts of cellulase and 3-18 parts of acid protease by weight.

8. The fermentation composition according to claim 1, characterized in that It also includes an acid-base regulator, which is selected from one or more of citric acid, sodium hydroxide, and sodium bicarbonate.

9. A method for preparing the sleep-aiding fermented composition for prolonging slow-wave sleep duration according to any one of claims 1 to 8, characterized in that: The steps include: (1) Mixing the fruiting bodies of Cicadae Concolor, Hydrangea, Gastrodia elata and water, and adjusting the pH to obtain a Chinese medicine mixed solution; (2) adding an enzyme preparation to the traditional Chinese medicine mixture for enzymatic hydrolysis, adjusting the pH, and obtaining an enzymatic hydrolyzate; (3) heating the enzymatic hydrolyzate to inactivate the enzyme and extract the enzymatic hydrolyzate to obtain an enzyme-inactivated extract; (4) adding a fermentation agent to the enzyme-killed extract to carry out fermentation to obtain a fermentation liquid; (5) The fermentation liquid is subjected to solid-liquid separation to obtain a fermentation clear liquid.

10. The method for preparing the fermentation composition according to claim 9, characterized in that: In step (2), the temperature of enzymatic hydrolysis is 40-65°C, and the time of enzymatic hydrolysis is 50-100 min; in step (3), the temperature of enzyme inactivation extraction is 90-110°C, and the time of enzyme inactivation extraction is 20-80 min; in step (4), the temperature of fermentation is 20-40°C, and the time of fermentation is 32-60 h.

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