Method for improving germination rate of Chinese herbal medicine podophyllum hexandrum seeds

Abstract

The invention discloses a method for increasing the germination rate of Chinese herbal medicine podophyllum hexandrum seeds, which comprises the following steps: cleaning the full podophyllum hexandrum seeds, putting into a beaker, adding a proper amount of water, stirring for 2.5-3.5 hours by using a magnetic stirrer at the rotating speed of 900-1100r / min, soaking in ethanol with the volume concentration of 75%, disinfecting for 1 + / -0.5 minutes, and washing with distilled water; and putting the podophyllum hexandrum seeds into a culture dish for culture germination treatment or sowing the podophyllum hexandrum seeds into a flowerpot for sowing germination treatment. Water flow generated by the rotor of the magnetic stirrer and the rotor are used for providing slow and soft friction force for seeds and providing long-time friction for seed coats, the compact and thickened seed coat structure of podophyllum hexandrum is effectively broken and the dormancy of the seeds is relieved under the condition that the seed structure is not damaged, the seed germination time can be obviously shortened, and the seed germination rate is improved. The method has the advantages that the treatment time is short, and the treatment is simple and convenient.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicinal material cultivation, and in particular to a method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia. Background Art

[0002] Peach-leaf lotus is a perennial herb of the Berberidaceae family. It is native to Yunnan, Sichuan, Tibet, Gansu, Qinghai, and Shaanxi, and grows in forest wetlands, under forests, in shrubs, or in grass. Peach-leaf lotus has been included in the "Red List of Chinese Species", "Red Data of Chinese Plants", and "List of Chinese Rare and Endangered Plants", and is a national second-class protected plant. The 2020 edition of the "Chinese Pharmacopoeia" includes it under the name "small-leaf lotus", and its medicinal part is the fruit.

[0003] Although the fruit of P. truncatum has a high seed yield, its seeds have strong dormancy characteristics. Under natural conditions, the dormancy period is as long as 6 to 10 months before they can complete physiological ripening and germinate. Studies have shown that long-term seed coat and endosperm restrictions, mechanical dormancy, hypocotyl dormancy, germination inhibitors and physiological ripening lead to unstable germination of P. truncatum seeds. The seed coat of P. truncatum is mainly composed of three layers, namely the cuticle, palisade stone cell layer and spongy tissue layer. The cell walls of these structures are usually thickened to a certain extent, resulting in greater mechanical constraints on seed germination, hindering the air permeability and water permeability of the seeds, causing seed dormancy, resulting in slow germination and low germination rate.

[0004] At present, the main methods to break the dormancy of Psoralea corylifolia are low temperature stratification and hormone treatment. These methods have the problems of long treatment time, cumbersome treatment process, low seed germination rate, etc. So far, there is no efficient method to break the dormancy of Psoralea corylifolia, which seriously hinders the artificial cultivation and development and utilization of Psoralea corylifolia.

[0005] Therefore, breaking the dormancy of Psoralea corylifolia seeds is a bottleneck in Psoralea corylifolia cultivation technology and is also an urgent problem that needs to be solved in the development of its cultivation technology. Summary of the invention

[0006] The technical problem to be solved by the present invention is to provide a method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia, which effectively breaks the dormancy of Psoralea corylifolia and shortens the germination time of Psoralea corylifolia seeds, while preventing damage to the seeds and improving the seed germination rate.

[0007] The present invention provides a method for improving the germination rate of seeds of Chinese medicinal material Psoralea corylifolia, comprising the following steps:

[0008] (1) Seed selection: Select seeds of Peach 77 with full seeds and uniform size;

[0009] (2) Cleaning: Place the seeds in a beaker, add a small amount of detergent, and stir with a glass rod for 3 to 10 minutes to remove impurities on the seed surface;

[0010] (3) Stirring: Place the cleaned Psoralea corylifolia seeds in a beaker, add an appropriate amount of water, and stir with a magnetic stirrer at a speed of 900 to 1100 r / min for 2.5 to 3.5 h;

[0011] (4) Disinfection: Soak the stirred seeds in 75% ethanol for 1±0.5 min, and then rinse with distilled water 2 to 3 times;

[0012] (5) Germination: The seeds of Psoralea corylifolia are cultured and germinated or sown and germinated.

[0013] Preferably, the rotation speed of the magnetic stirrer in step (3) is 1000 r / min, and the stirring time is 3 h.

[0014] Preferably, in step (3), the volume of the beaker is 100 ml, 10 to 20 ml of water is added, and 5 to 10 Psoralea corylifolia seeds are processed each time.

[0015] Preferably, the magnetic stirrer in step (3) is a multi-station magnetic stirrer.

[0016] Preferably, the operation of the culture germination treatment in step (5) is: placing the seeds in a culture dish with a diameter of 9 cm covered with two layers of filter paper, adding 5 ml of ultrapure water to each culture dish, and then placing the culture dish in an artificial climate box with a temperature of 24-26°C and a relative humidity of 60-70%, and culturing at a constant temperature and humidity under the condition of alternating cycles of 12 hours of light and 12 hours of darkness.

[0017] Preferably, the operation of the sowing and germination treatment in step (5) is: sowing the stirred seeds into a flower pot with a diameter of 15 to 20 cm and a height of 15 to 20 cm, with a sowing depth of 2 cm, and then placing the flower pot indoors for cultivation.

[0018] Preferably, the temperature of the artificial climate box in step (6) is 25° C. and the relative humidity is 65%.

[0019] Working principle of the present invention: The method of improving the germination rate of the seeds of the Chinese medicinal material Peach Seven of the present invention utilizes the water flow generated by the rotor of the magnetic stirrer and the rotor to rub the seeds for a long time, and rubs the seed coat by physical methods, thereby causing the cuticle on the surface of the seed coat to wear, destroying the rigid structure of the cell wall of the seed coat, reducing the mechanical binding force of the seeds, improving the water absorption performance, and enhancing the radicle growth ability, thereby releasing the dormancy of the seeds. In addition, the friction force generated by the method of rubbing the seeds with the rotor and the water flow is uniform, and can form a wrapped friction, which is convenient for all-round friction of the seed epidermis, and the friction force is slow and gentle, and will not seriously damage the seed structure.

[0020] Beneficial effects of the present invention: The present invention uses a magnetic stirrer to process the seeds of Psoralea corylifolia, and uses the water flow and rotor generated by the rotor of the magnetic stirrer to provide slow and gentle friction for the seeds, giving the seed coat a long time of friction, and effectively breaking the compact and thickened seed coat structure of Psoralea corylifolia without destroying the seed structure, relieving the dormancy of the seeds, and significantly shortening the seed germination time and improving the seed germination rate. This method has the advantages of short processing time and simple processing. In addition, the use of a multi-station magnetic stirrer for simultaneous operation is also suitable for large-scale seed processing and is suitable for standardized planting of Psoralea corylifolia. DETAILED DESCRIPTION

[0021] In order to make the technical solution of the present invention easier to understand, the technical solution of the present invention is now clearly and completely described in the form of specific embodiments.

[0022] Embodiment 1:

[0023] The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia of the present embodiment comprises the following steps:

[0024] (1) Seed selection: Select seeds of Peach 77 with full seeds and uniform size;

[0025] (2) Cleaning: Place the seeds in a beaker, add a small amount of detergent, and stir with a glass rod for 3 to 10 minutes to remove impurities on the seed surface;

[0026] (3) Stirring: Four groups of experiments were set up. Clean Psoralea corylifolia seeds were placed in a beaker and added with an appropriate amount of water. A magnetic stirrer was used to stir at a speed of 900-1100 r / min for 0 h, 1 h, 2 h, and 3 h, respectively, which were recorded as ck, J1, J2, and J3;

[0027] (4) Disinfection: Soak the stirred seeds in 75% ethanol for 1±0.5 min, and then rinse with distilled water 2 to 3 times;

[0028] (5) Germination culture: The seeds were placed in a 9-cm diameter culture dish covered with two layers of filter paper, and 5 ml of ultrapure water was added to each culture dish. The culture dishes were then placed in an artificial climate chamber at 25°C and 65% relative humidity, and cultured at a constant temperature and humidity with alternating cycles of 12 h of light and 12 h of darkness.

[0029] Embodiment 2:

[0030] The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia of the present embodiment comprises the following steps:

[0031] (1) Seed selection: Select seeds of Peach 77 with full seeds and uniform size;

[0032] (2) Cleaning: Place the seeds in a beaker, add a small amount of detergent, and stir with a glass rod for 3 to 10 minutes to remove impurities on the seed surface;

[0033] (3) Stirring: Four groups of experiments were set up. Clean Psoralea corylifolia seeds were placed in a beaker and added with an appropriate amount of water. The mixture was stirred with a magnetic stirrer at a speed of 1000 r / min for 0 h, 1 h, 2 h, and 3 h, respectively, which were recorded as ck, J1, J2, and J3;

[0034] (4) Disinfection: Soak the stirred seeds in 75% ethanol for 1 min, and then rinse with distilled water 2 to 3 times;

[0035] (5) Seeding and germination: Sow the stirred seeds into a flower pot with a diameter of 17 cm and a height of 15 cm, with a sowing depth of 2 cm, and then place the flower pot indoors for cultivation.

[0036] Characterization methods:

[0037] The determination and calculation formulas of seed germination related indicators are as follows:

[0038] Germination rate = the final number of germinated seeds / the number of tested seeds × 100%;

[0039] Germination potential = the number of seeds that germinate on the day reaching the peak value / the number of seeds tested × 100%;

[0040] Germination index = ∑(Gi / Di), Gi is the number of germinated seeds on the i-th day, and Di is the corresponding germination days.

[0041] Table 1 Effects of different treatments on seed germination of Psoralea corylifolia in Example 1

[0042]

[0043] The characterization results of Example 1 are shown in Table 1: After the seeds of Psoralea corylifolia were stirred by a magnetic stirrer for three hours, the germination rate reached 73.3%, an increase of 56.96% over the control; the germination potential and germination index were 20% and 3.41, respectively, an increase of 50.4% and 184.2% over the control. This shows that stirring with a magnetic stirrer can break the seed coat, break dormancy, and significantly improve the germination rate, germination potential and germination index of Psoralea corylifolia seeds.

[0044] Table 2 Effects of different treatments on seed germination of Psoralea corylifolia in Example 2

[0045]

[0046] The characterization results of Example 2 are shown in Table 2: After the seeds of Psoralea corylifolia were stirred by a magnetic stirrer for three hours, the germination rate reached 100%, an increase of 36.4% over the control; the germination potential and germination index were 46.7% and 10.7, respectively, an increase of 40.2% and 43.0% over the control. This shows that stirring with a magnetic stirrer can break the seed coat and break dormancy, significantly improving the germination rate, germination potential and germination index of Psoralea corylifolia after sowing.

[0047] It should be noted that the embodiments described herein are only partial embodiments of the present invention, rather than all implementations of the present invention, and the embodiments are only exemplary, and their role is only to provide a more intuitive and clear way to understand the content of the present invention, rather than to limit the technical solutions described in the present invention. Without departing from the concept of the present invention, all other implementation methods that can be thought of by ordinary technicians in this field without creative work, and other simple replacements and various changes to the technical solutions of the present invention, all belong to the protection scope of the present invention.

Claims

1. A method for improving the germination rate of seeds of Chinese medicinal material Psoralea corylifolia, characterized in that: The following steps are involved: (1) Seed selection: Select seeds of Peach 77 with full seeds and uniform size; (2) Cleaning: Place the seeds in a beaker, add a small amount of detergent, and stir with a glass rod for 3 to 10 minutes to remove impurities on the seed surface; (3) Stirring: Place the cleaned Psoralea corylifolia seeds in a beaker, add an appropriate amount of water, and stir with a magnetic stirrer at a speed of 900 to 1100 r / min for 2.5 to 3.5 h; (4) Disinfection: Soak the stirred seeds in 75% ethanol for 1±0.5 min, and then rinse with distilled water 2 to 3 times; (5) Germination: The seeds of Psoralea corylifolia are cultured and germinated or sown and germinated.

2. The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia according to claim 1, characterized in that: The rotation speed of the magnetic stirrer in step (3) is 1000 r / min and the stirring time is 3 h.

3. The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia as claimed in claim 1, characterized in that: In step (3), the volume of the beaker is 100 ml, 10 to 20 ml of water is added, and 5 to 10 Psoralea corylifolia seeds are processed each time.

4. The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia according to claim 1, characterized in that: The magnetic stirrer in step (3) is a multi-station magnetic stirrer.

5. The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia according to claim 1, characterized in that: The operation of the culture germination treatment in step (5) is as follows: the seeds are placed in a culture dish with a diameter of 9 cm covered with two layers of filter paper, 5 ml of ultrapure water is added to each culture dish, and then the culture dish is placed in an artificial climate box with a temperature of 24-26° C. and a relative humidity of 60-70%, and cultured at a constant temperature and humidity under the condition of alternating cycles of 12 hours of light and 12 hours of darkness.

6. The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia according to claim 1, characterized in that: The operation of the sowing and germination treatment in step (5) is as follows: sowing the stirred seeds into a flower pot with a diameter of 15 to 20 cm and a height of 15 to 20 cm, with a sowing depth of 2 cm, and then placing the flower pot indoors for cultivation.

7. The method for improving the germination rate of seeds of the Chinese medicinal material Psoralea corylifolia according to claim 1, characterized in that: The temperature of the artificial climate box in step (6) is 25° C. and the relative humidity is 65%.

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