Retinol nano-liposome with wrinkle removing effect and preparation method and application of freeze-dried powder of retinol nano-liposome

Abstract

The invention discloses a retinol nano-liposome with a wrinkle removing effect and a preparation method and application of freeze-dried powder of the retinol nano-liposome, and the retinol nano-liposome comprises the following components: 4.8%-5.2% of retinol, 45%-55% of a humectant, 20%-30% of a solvent, 5%-15% of an emollient, 5%-15% of a lubricant, 3%-4% of a coating material, 0.8%-1.2% of an emulsifier and 0.8%-1.2% of a moisturizing preservative. The retinol nano-liposome freeze-dried powder prepared from the arranged retinol nano-liposome and other auxiliary materials can be used as a whitening skin care product for skin care, so that the function of promoting permeation of active matters of the nano-liposome can be exerted, retinol can be well absorbed, and the effects of removing wrinkles, tendering skin and protecting skin can be exerted to the greatest extent; and the retinol can be protected from being influenced by oxidation, the retinol can be stored at normal temperature for a long time, and the problem of agglomeration of nano materials is effectively avoided. And after long-term use by a user, the product shows good effects of removing wrinkles, resisting aging, removing yellowness, brightening, tightening, softening and smoothing skin.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, in particular to a method for preparing and applying a retinol nanoliposome having wrinkle-removing effect and a freeze-dried powder thereof. Background Art

[0002] With the rapid development of science and technology, instruments and methodology, people have a deeper understanding of themselves. The research on skin physiology and pathology has penetrated into the level of genetics and molecular biology, which has greatly promoted the development of related bio-pharmaceutical industries. Many biologically active animal and plant components and biological polymer components have been applied to the bio-pharmaceutical industry, achieving huge social and economic benefits.

[0003] In recent years, retinol, which has specific skin active functions, has been widely used in skin care products. It is a vitamin substance, and it is difficult for it to pass through the skin barrier to be effectively absorbed, so it is difficult to play a good role; the difficulty of transdermal absorption is not the size of the molecular weight, but the fact that there is a barrier on the surface of the skin that is difficult to break through, both waterproof and oil-proof, and it is difficult for ordinary substances to penetrate. In addition, retinol is easily oxidized, thus losing its activity, and needs to be stored at low temperatures and away from light.

[0004] If retinol cannot be effectively absorbed, its physiological effects such as wrinkle removal, skin rejuvenation and whitening cannot be well achieved. In addition, retinol is easily oxidized, which will lead to a lot of active ingredients being used but very little effect. Therefore, the preservation and absorption of retinol is an urgent problem to be solved in the beauty industry. Making retinol into nanoliposomes can effectively solve the absorption problem and avoid oxidation; however, nanomaterials are very fine particles and are easily agglomerated in liquid state. Making them into freeze-dried powder can effectively solve the agglomeration problem of nanomaterials.

[0005] In summary, in view of the fact that retinol is difficult to be effectively absorbed through the skin, is easily oxidized and difficult to preserve, and nanomaterials are prone to agglomeration in liquid state, the present invention provides a method for preparing and using retinol nanoliposomes with anti-wrinkle effects and their freeze-dried powder. Summary of the invention

[0006] In order to effectively solve the problems in the background technology and overcome the technical defects in the prior art, the present invention provides the following technical solutions:

[0007] A retinol nanoliposome with anti-wrinkle effect, comprising the following components:

[0008] Retinol 4.8%-5.2%, moisturizer 45%-55%, solvent 20%-30%, emollient 5%-15%, lubricant 5%-15%, dressing material 3%-4%, emulsifier 0.8%-1.2%, moisturizing preservative 0.8%-1.2%.

[0009] As a preferred technical solution of the present invention, the following components are included:

[0010] Retinol 4.8%, moisturizer 48%, solvent 20%, emollient 11%, lubricant 11%, dressing material 3%, emulsifier 1.3%, moisturizing preservative 0.9%.

[0011] As a preferred technical solution of the present invention, the following components are included:

[0012] Retinol 5%, moisturizer 52%, solvent 22%, emollient 10%, lubricant 7.5%, dressing material 2.5%, emulsifier 1%, moisturizing preservative 1%.

[0013] As a preferred technical solution of the present invention, the following components are included:

[0014] Retinol 5.2%, moisturizer 43%, solvent 23%, emollient 12%, lubricant 12%, dressing material 2.4%, emulsifier 1.2%, moisturizing preservative 1.2%.

[0015] Furthermore, a method for preparing retinol nanoliposomes having anti-wrinkle effect and freeze-dried powder thereof comprises the following steps:

[0016] Step 1: Weigh corresponding amounts of retinol, moisturizer, solvent, emollient, lubricant, dressing material, emulsifier, moisturizing preservative, pour them into a container, stir and mix, and process under high pressure to prepare retinol nanoliposomes;

[0017] Step 2: Select a volumetric flask, irrigate 1g of liquid into a 3g bottle, irrigate 2g of liquid into a 5g bottle, fill all the liquid in step 1, and then add the filling material;

[0018] Step 3: Place the volumetric flask in a freeze dryer, set the temperature at -48°C, freeze dry the 5g bottle for 24 hours, and freeze dry the 3g bottle for 18 hours;

[0019] Step 4: Slowly heat the volumetric flask in step 3 to 45° C., and then evacuate the volumetric flask to form retinol nanoliposome freeze-dried powder.

[0020] Furthermore, the retinol nanoliposome and the freeze-dried powder thereof are used in cosmetics.

[0021] Compared with the prior art, the present invention uses the retinol nanoliposomes and then prepares the retinol nanoliposome freeze-dried powder with other auxiliary materials, which can be used as a whitening skin care product for skin care. This can not only play the role of promoting the penetration of active substances in the nanoliposomes, so that retinol can be better absorbed and play the maximum wrinkle removal, skin rejuvenation and skin care effects, but also protect retinol from oxidation, and can be stored at room temperature for a long time, and effectively avoid the problem of agglomeration of nanomaterials. And after long-term use by users, this product shows good effects of wrinkle removal, anti-aging, yellow removal, brightening, firming, smoothing and delicate skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is an electron microscope image of the nanoliposome in the present invention.

[0023] Figure 2 Graph showing the particle size distribution of the nanoliposomes of the present invention.

[0024] Figure 3 This is a product diagram of the retinol nanoliposomes of the present invention;

[0025] Figure 4 This is a picture of the freeze-dried powder product of retinol nanoliposomes in the present invention. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution in the embodiment of the present invention will be further clearly and completely described below. Obviously, the described embodiment is only a part of the embodiment of the present invention, not all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The present invention provides a technical solution: a retinol nanoliposome freeze-dried powder with wrinkle removal effect, comprising the following components:

[0028] Retinol 4.8%-5.2%, moisturizer 45%-55%, solvent 20%-30%, emollient 5%-15%, lubricant 5%-15%, dressing material 3%-4%, emulsifier 0.8%-1.2%, moisturizing preservative 0.8%-1.2%.

[0029] Among them, moisturizers include but are not limited to glycerin, propylene glycol, sorbitol, betaine and polyethylene glycol;

[0030] Solvents: including but not limited to water, alcohol, liquid alkanes, cyclohexanone, amyl acetate and ethylene glycol butyl ether, etc.;

[0031] Emollients: including but not limited to cetyl ethylhexanoate, myristyl myristate, caprylic capric glyceride, oleyl oleate, hexyl laurate, isopropyl palmitate, PEG-6 caprylic / capric triglyceride, PEG-60 corn oil glyceryl esters and PEG-7 olive oil ester, etc.;

[0032] Lubricants: including but not limited to butanediol, mineral lubricants, synthetic lubricants, animal and vegetable oils, and water-based liquids;

[0033] Coating materials: including but not limited to beta-cyclodextrin, alpha-cyclodextrin, gamma-cyclodextrin, hydroxypropyl beta-cyclodextrin, methyl beta-cyclodextrin, hydroxypropyl gamma-cyclodextrin, soybean-derived lecithin, egg yolk-derived lecithin, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, egg yolk phospholipids, phosphatidylglycerol, lecithin particles, phosphatidylethanolamine, phosphatidylinositol phosphate and all hydrogenated lecithins, etc.;

[0034] Emulsifiers include but are not limited to: Span series, Tween series, nonionic emulsifiers, anionic emulsifiers and cationic emulsifiers, etc.;

[0035] Moisturizing preservatives: including but not limited to: glycol moisturizing preservatives, such as pentylene glycol, hexylene glycol, caprylyl glycol, etc., ethylhexylglycerin and caprylhydroxamic acid, etc.;.

[0036] Embodiment 1:

[0037] Contains the following ingredients:

[0038] Retinol 4.8%, moisturizer 48%, solvent 20%, emollient 11%, lubricant 11%, dressing material 3%, emulsifier 1.3%, moisturizing preservative 0.9%.

[0039] Embodiment 2:

[0040] Contains the following ingredients:

[0041] Retinol 5%, moisturizer 52%, solvent 22%, emollient 10%, lubricant 7.5%, dressing material 2.5%, emulsifier 1%, moisturizing preservative 1%.

[0042] Embodiment 3:

[0043] Contains the following ingredients:

[0044] Retinol 5.2%, moisturizer 43%, solvent 23%, emollient 12%, lubricant 12%, dressing material 2.4%, emulsifier 1.2%, moisturizing preservative 1.2%.

[0045] Embodiment 4:

[0046] A method for preparing retinol nanoliposomes with anti-wrinkle effect and freeze-dried powder thereof comprises the following steps:

[0047] Step 1: Weigh corresponding amounts of retinol, moisturizer, solvent, emollient, lubricant, dressing material, emulsifier, moisturizing preservative, pour them into a container, stir and mix, and process under high pressure to prepare retinol nanoliposomes;

[0048] Step 2: Select a volumetric flask, irrigate 1g of liquid into a 3g bottle, irrigate 2g of liquid into a 5g bottle, fill all the liquid in step 1, and then add the filling material;

[0049] Step 3: Place the volumetric flask in a freeze dryer, set the temperature at -48°C, freeze dry the 5g bottle for 24 hours, and freeze dry the 3g bottle for 18 hours;

[0050] Step 4: Slowly heat the volumetric flask in step 3 to 45° C., and then evacuate the volumetric flask to form retinol nanoliposome freeze-dried powder.

[0051] The invention relates to the application of retinol nanoliposomes and freeze-dried powder thereof having whitening effect in cosmetics, specifically, the application of retinol nanoliposomes and freeze-dried powder thereof in cosmetic raw materials.

[0052] Specifically, it is divided into two parts: first, it is made into an emulsion, which solves the problem of retinol absorption and stability; then it is made into freeze-dried powder, which solves the problem of nanomaterial agglomeration.

[0053] 1. Comparative Proportion

[0054] The composition is composed of the following raw materials in parts by weight:

[0055] Moisturizer 48%, solvent 36%, emollient 10%, emulsifier 3.5%, auxiliary emulsifier 1%, auxiliary moisturizer 1.5%;

[0056] Then, the preparation was carried out according to the method in Example 4, and the retinol nanoliposomes and freeze-dried powders thereof in Examples 1 to 3 were prepared at the same time.

[0057] 2. Experimental Examples

[0058] (1) First, the skin care effects of the serum with added retinol liposomes and the serum with added untreated retinol were compared: the nanoliposomes of Examples 1 to 3 were added to the moisturizing serum (the addition ratio was 6%, wherein the retinol content was 0.288%, 0.300% and 0.312%, i.e., Examples 1, 2 and 3), and then a portion of the moisturizing serum was taken and 0.5% retinol was added (i.e., the untreated group), and a comparative experiment on the skin improvement effect was performed;

[0059] Specifically: Volunteers were first selected, all of whom had wrinkles and dull skin to varying degrees, aged 35-50 years old, a total of 48 people, and were divided into 4 groups, corresponding to Examples 1 to 3 and the comparative example, with 12 people in each group;

[0060] The people in each group were respectively coated with the corresponding nanoliposome freeze-dried powder of Examples 1 to 3 and the comparative example, once in the morning and once in the evening, and then the application lasted for four weeks and then observed;

[0061]

[0062]

[0063] (2) Secondly, the skin care effects of the essence with retinol liposome freeze-dried powder added and the essence without retinol liposome freeze-dried powder were compared. The nanoliposome freeze-dried powder of Examples 1 to 3 was added to the moisturizing essence (addition ratio was 6%) to conduct skin improvement effect experiments, and the control example did not add retinol;

[0064] Specifically: Volunteers were first selected, all of whom had wrinkles and dull skin to varying degrees, aged 35-50 years old, a total of 48 people, and were divided into 4 groups, corresponding to Examples 1 to 3 and the comparative example, with 12 people in each group;

[0065] The people in each group were respectively coated with the corresponding nanoliposome freeze-dried powder of Examples 1 to 3 and the comparative example, once in the morning and once in the evening, and then the application lasted for four weeks and then observed;

[0066]

[0067]

[0068] (3) Again, the freeze-dried nanoliposome powders in Examples 1 to 3 were placed for half a year and then added to the moisturizing essence (addition ratio was 6%) and then compared with the moisturizing essence of the comparative example in a skin improvement effect experiment;

[0069] Specifically: Volunteers were first selected, all of whom had wrinkles and dull skin to varying degrees, aged 35-50 years old, a total of 48 people, and were divided into 4 groups, corresponding to Examples 1 to 3 and the comparative example, with 12 people in each group;

[0070] The people in each group were respectively applied with the corresponding essence of the nanoliposome freeze-dried powder of Examples 1 to 3 and the moisturizing essence of the comparative example, once in the morning and once in the evening, and then applied for four weeks and observed;

[0071]

[0072]

[0073] In summary, in the present invention, by the retinol nanoliposomes provided, the retinol nanoliposome freeze-dried powder made with other auxiliary materials can be used as a whitening skin care product for skin care. In this way, the function of promoting the penetration of active substances of nanoliposomes can be brought into play, the transdermal absorption rate can be greatly improved, retinol can be better absorbed and the maximum wrinkle removal, skin rejuvenation and skin care effects can be brought into play, and retinol can be protected from the influence of oxidation, and can also be stored at room temperature for a long time, and the problem of agglomeration of nano materials can be effectively avoided. After the user uses it for a period of time, this product shows good wrinkle removal and anti-aging, yellow removal and brightening, firming and smooth and delicate skin effects. Moreover, after the freeze-dried powder is placed for a long time, its effect is substantially unaffected. In addition, the effect of the composition made in Embodiment 3 of the present invention is the best.

[0074] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A retinol nanoliposome with anti-wrinkle effect, Features: Contains the following ingredients: Retinol 4.8%-5.2%, moisturizer 45%-55%, solvent 20%-30%, emollient 5%-15%, lubricant 5%-15%, dressing material 3%-4%, emulsifier 0.8%-1.2%, moisturizing preservative 0.8%-1.2%.

2. The retinol nanoliposome with anti-wrinkle effect according to claim 1, Features: Contains the following ingredients: Retinol 4.8%, moisturizer 48%, solvent 20%, emollient 11%, lubricant 11%, dressing material 3%, emulsifier 1.3%, moisturizing preservative 0.9%.

3. The retinol nanoliposome with anti-wrinkle effect according to claim 1, Features: Contains the following ingredients: Retinol 5%, moisturizer 52%, solvent 22%, emollient 10%, lubricant 7.5%, dressing material 2.5%, emulsifier 1%, moisturizing preservative 1%.

4. The retinol nanoliposome with anti-wrinkle effect according to claim 1, Features: Contains the following ingredients: Retinol 5.2%, moisturizer 43%, solvent 23%, emollient 12%, lubricant 12%, dressing material 2.4%, emulsifier 1.2%, moisturizing preservative 1.2%.

5. A method for preparing the wrinkle-removing retinol nanoliposome and its freeze-dried powder according to any one of claims 1 to 4, Features: The following steps are involved: Step 1: Weigh corresponding amounts of retinol, moisturizer, solvent, emollient, lubricant, dressing material, emulsifier, moisturizing preservative, pour them into a container, stir and mix, and process under high pressure to prepare retinol nanoliposomes; Step 2: Select a volumetric flask, irrigate 1g of liquid into a 3g bottle, irrigate 2g of liquid into a 5g bottle, fill all the liquid in step 1, and then add the filling material; Step 3: Place the volumetric flask in a freeze dryer, set the temperature at -48°C, freeze dry the 5g bottle for 24 hours, and freeze dry the 3g bottle for 18 hours; Step 4: Slowly heat the volumetric flask in step 3 to 45° C., and then evacuate the volumetric flask to form retinol nanoliposome freeze-dried powder.

6. Use of the retinol nanoliposomes with anti-wrinkle effect and the freeze-dried powder thereof in cosmetics as claimed in claim 5, Features: The retinol nanoliposome and the freeze-dried powder thereof are applied in cosmetics.

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