Eye care solution prepared from plant extracts and used for relieving cataract

Abstract

The invention discloses a cataract-relieving eye care solution prepared from plant extracts, which is prepared from the following raw materials in parts by weight: 0.025-0.05 part of grape seed extract, 0.125-0.25 part of long-time moxibustion cistanche deserticola extract, 2-4 parts of sodium chloride and 250-500 parts of Shihan spring water, and is prepared by the following steps: weighing the grape seed extract, wine-roasted cistanche deserticola water extract and sodium chloride in proportion; adding into Shihan spring water, and fully dissolving by ultrasonic waves; filtering and degerming by using a degerming membrane, and irradiating for two hours under ultraviolet light; the invention relates to the technical field of eye care solutions, and the eye care solution is mild in use condition and convenient to use, can achieve a good effect of relieving cataract without an operation, and has no toxic or side reaction to a human body due to the fact that functional components are all from natural products.

Description

Technical Field

[0001] The invention relates to the technical field of eye care solutions, in particular to an eye care solution for alleviating cataracts prepared from plant extracts. Background Art

[0002] Cataract is a blinding eye disease and is currently the leading cause of blindness in the world. The onset of cataracts is related to many factors, but so far, there is no consensus on the pathogenesis of cataracts. Currently, surgery is the main method for treating cataracts, but the high cost and requirements of surgical treatment mean that many cataract patients cannot receive good treatment. Therefore, extracting active components from traditional Chinese medicine to effectively prevent and treat cataracts is a way worth exploring.

[0003] As a traditional tonic precious Chinese medicinal material, Cistanche deserticola has a history of application of thousands of years. Studies have shown that Cistanche deserticola has the effects of nourishing the kidney and qi, delaying aging, etc. Cistanche deserticola contains polysaccharides, phenylethanol glycosides, and polyphenols. Cistanche deserticola has significant effects in the treatment of various diseases, but there is little research on its treatment of eye diseases. Modern medical research shows that Cistanche deserticola extract has anti-inflammatory, antioxidant, blood sugar-lowering and cancer cell-inhibiting effects. It can maintain the content of SOD, GSH and GSH-PX in vivo. These components are related to cataracts. Therefore, it is necessary to explore the treatment of eye diseases with Cistanche deserticola.

[0004] Grape seed extract contains abundant natural medicinal components. The most important one is resveratrol. Studies have shown that resveratrol may regulate the expression of SIR T1, further regulate the expression of downstream genes P53, forkhead transcription factor 1, and NF-κB, inhibit and delay the apoptosis of lens epithelial cells, and delay the occurrence and development of cataracts in diabetic rats. Therefore, it shows good prospects in the field of cataract treatment.

[0005] A single extract component cannot achieve a good effect in treating cataracts. This experiment combines Cistanche deserticola extract and grape seed extract to explore their therapeutic effects on cataracts, providing a path for Chinese herbal extracts in treating cataracts. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention provides an eye care solution for alleviating cataracts prepared from a plant extract, which solves the problem that the existing method for treating cataracts is mainly through surgical excision, but this method is expensive, time-consuming, and has certain risks.

[0007] To achieve the above objectives, the present invention is implemented through the following technical scheme: a plant extract is used to prepare an eye care solution for alleviating cataracts, comprising the following raw materials in parts by weight: 0.025-0.05 parts of grape seed extract, 0.125-0.25 parts of long-moxibustion Cistanche extract, 2-4 parts of sodium chloride and 250-500 parts of Shihan spring water.

[0008] Preferably, the following raw materials are included in parts by weight: 0.03 parts of grape seed extract, 0.15 parts of long-moxibustion Cistanche extract, 2.4 parts of sodium chloride and 300 parts of Shihan spring water.

[0009] Preferably, the method further comprises the following preparation steps:

[0010] S1. Weigh grape seed extract, wine-roasted Cistanche deserticola water extract and sodium chloride in proportion, add them into Shihan spring water, and fully dissolve them by ultrasonication;

[0011] S2. Filter the prepared solution through a sterilization membrane and sterilize it under ultraviolet light for two hours;

[0012] S3. Use a pipette to draw 2 ml of the sterilized solution, bottle it, and seal it.

[0013] Preferably, the preparation method of the grape seed extract is: adding distilled water to dried grape seeds at a solid-liquid ratio of 1:20, extracting at 60° C. for two hours, centrifuging and filtering to remove insoluble matter, and drying the supernatant to obtain the grape seed extract.

[0014] Preferably, the method for preparing the wine-roasted Cistanche deserticola water extract is as follows: Cistanche deserticola is moistened with rice wine and then dried, crushed and extracted with water, the solid-liquid ratio is 1:20, extracted at 80°C for 2 hours, centrifuged and filtered, and then spray-dried to obtain the wine-roasted Cistanche deserticola water extract.

[0015] Preferably, the rare spring water is natural soda water.

[0016] Beneficial Effects

[0017] The present invention provides a plant extract for preparing an eye care solution for alleviating cataracts, which has the following beneficial effects:

[0018] The present invention has mild conditions for use and is easy to use. It can achieve a good effect of alleviating cataracts without surgery. Moreover, since the effective ingredients are all from natural products, they have no toxic side effects on the human body. The grape seed extract used is rich in antioxidant components, which helps to reduce the impact of oxidative stress on the eyes and delay the development of cataracts. The Cistanche extract is rich in multiple active ingredients, which helps to improve eye blood circulation and reduce eye fatigue, thereby assisting in alleviating cataract symptoms. Sodium chloride serves as an osmotic pressure regulator to maintain the osmotic balance between the solution and the eye tissue and ensure the physiological compatibility of the eye care solution. Shihan spring water, as a natural soda water, has good solubility and buffering capacity, which helps to improve the bioavailability of other ingredients while maintaining the pH value of the eye care solution stable. Through scientific and reasonable ingredient ratios and preparation processes, the product is highly efficient, safe and convenient. For cataract patients, this is a very practical and targeted eye care product. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the process of the present invention.

[0020] Figure 2a This is a schematic diagram of the RCFBF cytotoxicity test of the formulation of the present invention.

[0021] Figure 2b This is a schematic diagram of the RCFBF cytotoxicity test of Formulation 2 of the present invention.

[0022] Figure 2c This is a schematic diagram of the RCFBF cytotoxicity test of Formulation 3 of the present invention.

[0023] Figure 2d This is a schematic diagram of the RCFBF cytotoxicity test of Formulation 4 of the present invention.

[0024] Figure 2e This is a schematic diagram of the RCFBF cytotoxicity test of Formulation 5 of the present invention.

[0025] Figure 2f This is a schematic diagram of the RCFBF cytotoxicity test of Formulation 6 of the present invention.

[0026] Figure 3a This is a schematic diagram of the RCFC cytotoxicity test of formulation 1 of the present invention.

[0027] Figure 3b This is a schematic diagram of the RCFC cytotoxicity test of Formulation 2 of the present invention.

[0028] Figure 3c This is a schematic diagram of the RCFC cytotoxicity test of formulation three of the present invention.

[0029] Figure 3d This is a schematic diagram of the RCFC cytotoxicity test of Formulation 4 of the present invention.

[0030] Figure 3e This is a schematic diagram of the RCFC cytotoxicity test of Formulation 5 of the present invention.

[0031] Figure 3f This is a schematic diagram of the RCFC cytotoxicity test of Formulation 6 of the present invention.

[0032] Figure 4a This is a schematic diagram of the RCFBF cell scratch test of the present invention.

[0033] Figure 4b This is a schematic diagram of the RCFBF cell scratch test of Formula 2 of the present invention.

[0034] Figure 4c This is a schematic diagram of the RCFBF cell scratch test of formulation three of the present invention.

[0035] Figure 4d This is a schematic diagram of the RCFBF cell scratch test of Formula 4 of the present invention.

[0036] Figure 4e This is a schematic diagram of the RCFBF cell scratch test of Formula 5 of the present invention.

[0037] Figure 4f This is a schematic diagram of the RCFBF cell scratch test of Formula 6 of the present invention.

[0038] Figure 5a This is a schematic diagram of the RCFC cell scratch test of Formula 1 of the present invention.

[0039] Figure 5b This is a schematic diagram of the RCFC cell scratch test of Formula 2 of the present invention.

[0040] Figure 5c This is a schematic diagram of the RCFC cell scratch test of formulation three of the present invention.

[0041] Figure 5d This is a schematic diagram of the RCFC cell scratch test of Formula 4 of the present invention.

[0042] Figure 5e This is a schematic diagram of the RCFC cell scratch test of Formula 5 of the present invention.

[0043] Figure 5f This is a schematic diagram of the RCFC cell scratch test of Formula 6 of the present invention. DETAILED DESCRIPTION

[0044] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] See also Figure 1 -2. The present invention provides a technical solution:

[0046] 1. Raw and auxiliary materials

[0047] 1.1 Sample preparation

[0048] Formula 1: Grape seed extract: 100, 500, 1000, 2000, 5000 μg / mL;

[0049] Formula 2: Grape seed extract: Vitamin C = 10: 1 = 100, 500, 1000, 2000, 5000 μg / mL;

[0050] Formula 3: Wine-roasted Cistanche deserticola water extract: 100, 500, 1000, 2000, 5000 μg / mL;

[0051] Formula 4: Fresh Cistanche deserticola polysaccharide: 0.05, 0.1, 0.5, 1, 3 mg / mL;

[0052] Formula 5: Grape seed extract: wine-roasted Cistanche deserticola water extract 1:1 = 100, 500, 1000, 2000, 5000 μg / mL;

[0053] Formula 6: Steamed Cistanche deserticola water extract: 100, 500, 1000, 2000, 5000 μg / mL;

[0054] Shihanquan concentrate; sodium chloride.

[0055] 1.2 Reagents and consumables

[0056] 1mL syringe, sterile filter, rubber gloves, sterile cell culture flask, 15mL centrifuge tube, anhydrous ethanol, 96-well cell culture plate, pipette, pipette tip, cell culture medium, DMSO, trypsin digestion solution, PBS buffer, fetal bovine serum, liquid nitrogen, carbon dioxide, RCFBF cells, RCFC cells.

[0057] 1.3 Instruments and Equipment

[0058] Constant temperature cell culture incubator, sterile operating table, microplate reader, optical microscope, refrigerated centrifuge, liquid nitrogen tank.

[0059] 2 Experimental methods

[0060] Rabbit corneal anterior stromal fibroblasts (RCFBF) and rat conjunctival fibroblasts (RCFC) were used.

[0061] 2.1 Cytotoxicity assay

[0062] 2.1.1 RCFBF cells

[0063] RCFBF cells were cultured in F12 medium containing 5% fetal calf serum (FCS) at 37°C (5% CO2 and saturated humidity) for 48-72h. RCFBF cells in the logarithmic phase were collected and the cell suspension concentration was adjusted to 1×10 5 cells / mL. RCFBF cells were added to a 96-well plate (with sterile PBS in the edge wells), cultured at 37°C for 12h, and then treated with different concentrations of the formula samples. Complete medium was used as a negative control group. After incubation for 24h, 6ml of a culture bottle can be used to spot cells in 60 wells, 100μl per well, grown on the plate for 12h, and the sample was added to 200μl per well for 4h. The culture medium was removed and 100μL of MTT (0.5mg / mL) was added to each well and cultured for another 4h. The supernatant was then aspirated and 150μL of dimethyl sulfoxide (DMSO) was added to the 96-well plate, which was placed on a shaker to completely dissolve the crystals at a low speed. The absorbance at 570 nm was measured by an ELISA reader. Cell viability was calculated according to the following formula:

[0064] Cell viability (%) = Abs sample / Abs control × 100

[0065] Concentration gradient setting: 100 μl, 60 μl, 20 μl sample, add culture medium to 100 μl.

[0066] 2.1.2 RCFC cells

[0067] RCFC cells were cultured in F12 medium containing 5% fetal calf serum (FCS) at 37°C (5% CO2 and saturated humidity) for 48-72h. RCFC cells in the logarithmic phase were collected and the cell suspension concentration was adjusted to 1×10 5 cells / mL. RCFC cells were added to a 96-well plate (with sterile PBS in the edge wells), cultured at 37°C for 12h, and then treated with different concentrations of the formula samples. Complete medium was used as a negative control group. After incubation for 24h, 6ml of a culture flask can be used to spot cells in 60 wells, 100μl per well, grown on the plate for 12h, and the sample was added to 200μl per well for 4h. The culture medium was removed and 100μL of MTT (0.5mg / mL) was added to each well and cultured for another 4h. The supernatant was then aspirated and 150μL of dimethyl sulfoxide (DMSO) was added to the 96-well plate, which was placed on a shaker to completely dissolve the crystals at a low speed. The absorbance at 570 nm was measured by an ELISA reader. Cell viability was calculated according to the following formula:

[0068] Cell viability (%) = Abs sample / Abs control × 100

[0069] Concentration gradient setting: 100 μl, 60 μl, 20 μl sample, add culture medium to 100 μl.

[0070] 2.2 Cell scratch assay

[0071] 2.2.1 RCFBF cells

[0072] RCFBF cells were cultured in F12 medium containing 5% fetal calf serum (FCS) at 37°C (5% CO2 and saturated humidity) for 48-72h. RCFBF cells in the logarithmic phase were collected and the cell suspension concentration was adjusted to 1×10 5 cells / mL. RCFBF cells were added to a 12-well plate, cultured at 37°C for 12h, scratched with a syringe, and then treated with the formula sample. Complete culture medium was used as a negative control group. The cells were observed and photographed using an inverted cell microscope.

[0073] Concentration setting: 100 μl (96-well plate) or 500 μl (12-well plate) sample per well.

[0074] 2.2.2 RCFC cells

[0075] RCFC cells were cultured in F12 medium containing 5% fetal calf serum (FCS) at 37°C (5% CO2 and saturated humidity) for 48-72h. RCFC cells in the logarithmic phase were collected and the concentration of the cell suspension was adjusted to 1×10 5 cells / mL. RCFC cells were added to a 12-well plate, cultured at 37°C for 12h, scratched with a syringe, and then treated with the formula sample. Complete culture medium was used as a negative control group. The cells were observed and photographed using an inverted cell microscope.

[0076] Concentration setting: 100 μl (96-well plate) or 500 μl (12-well plate) sample per well.

[0077] 3 Experimental results

[0078] 3.1 Physical and chemical properties

[0079] Table 1 Conductivity, osmotic pressure, surface tension and pH value of samples

[0080]

[0081] 3.2 Cytotoxicity

[0082] 3.2.1 RCFBF cells.

[0083] Formula 1: Grape seed extract, such as Figure 2a As shown in the experimental results, it can be seen that grape seed extract has low toxicity at low concentrations and has certain toxicity at high concentrations, but the toxicity is not strong, and the cell survival rate is still around 80%. Therefore, it can be used as an eye care solution, but grape seed extract has a darker color, and too high a concentration will affect the color of the product.

[0084] Formula 2: Grape seed extract: vitamins, such as Figure 2b As shown in the figure, compared with Formula 1, Formula 2 adds a certain amount of vitamin C. From the results, it can be seen that the cytotoxicity is lower than that of Formula 1, but the overall trend is still that the toxicity is lower at low concentrations and the cytotoxicity gradually increases at high concentrations. However, the cell survival rate is similar to that of the blank sample. This shows that low concentrations of grape seed extract and vitamin C help cell proliferation.

[0085] Formula 3: Wine-roasted Cistanche deserticola water extract, such as Figure 2c As shown, it can be seen from the experimental results that the Cistanche extract is helpful for cell culture and has less toxicity at low concentrations, but the cytotoxicity increases when the concentration increases, but the cell survival rate is above 80%, so the wine-roasted Cistanche extract can be used as an eye care solution product.

[0086] Formula 4: Fresh Cistanche deserticola polysaccharide, such as Figure 2d As shown, from the experimental results, it can be seen that Cistanche polysaccharide promotes cell proliferation, especially when the polysaccharide concentration is 100 and 500 μg / ml, the promotion effect on cells is the greatest, and the survival rate is lower at lower and higher concentrations. This shows that at lower concentrations, the promotion effect on cells is not obvious, and at higher concentrations, the cytotoxicity is greater. Therefore, Cistanche polysaccharide can be used as an eye care solution, but the sample is easily contaminated with bacteria, which can be verified by the following scratch experiment. As an eye care solution, antibacterial issues should be considered;

[0087] Formula 5: Grape seed extract: wine roasted Cistanche deserticola water extract, such as Figure 2e As shown, formula five is grape seed extract + wine-roasted Cistanche deserticola water extract. From the results, it can be seen that the mixture of the two substances has a synergistic effect on cell proliferation. Moreover, after adding Cistanche deserticola extract, the cytotoxicity of grape seed extract is significantly reduced, and the cell activity is greater than that of a single Cistanche deserticola extract. Therefore, formula five is suitable for eye care solution.

[0088] Formula 6: Steamed Cistanche deserticola water extract, such as Figure 2f As shown in the results, the toxicity of steamed Cistanche deserticola water extract is similar to that of wine-roasted Cistanche deserticola water extract. This indicates that the processing method of Cistanche deserticola has nothing to do with cytotoxicity, and both can be used as eye care solutions, but the concentration should not be too high.

[0089] 3.2.2 RCFC cells

[0090] Formula 1: Grape seed extract, such as Figure 3a As shown in the results, it can be seen that grape seed extract promotes cell growth at low concentrations, and has higher cytotoxicity at higher concentrations. Therefore, when used as an eye care solution, the concentration issue should be considered. At low concentrations, the sample color is lighter and has less impact on the product.

[0091] Formula 2: Grape seed extract: Vitamin C, such as Figure 3b As shown, it can be seen from the results that grape seed extract + vitamin C promotes cell growth at low concentrations, and when the concentration increases, the cytotoxicity increases. When used as an eye care solution, the concentration issue should be considered.

[0092] Formula 3: Wine-roasted Cistanche deserticola water extract, such as Figure 3c As shown in the results, it can be seen that the wine-roasted Cistanche deserticola water extract has an increasing effect on cell growth with increasing concentration, indicating that the Cistanche deserticola extract promotes cell proliferation and can be used as an eye care solution. However, from the rabbit corneal anterior stroma fibroblast experiment, the Cistanche deserticola extract is easily contaminated with bacteria, so the antibacterial problem should be considered.

[0093] Formula 4: Fresh Cistanche deserticola polysaccharide, such as Figure 3d As shown in the results, it can be seen that the toxicity of Cistanche deserticola polysaccharide to cells is relatively low. At low concentrations, it has a promoting effect on cells. When the concentration increases, the toxicity does not increase significantly, so it can be used as an eye care solution. However, this sample is easily contaminated with bacteria, so when used as an eye care solution, the antibacterial effect of the product should be considered first.

[0094] Formula 5: Grape seed extract: wine roasted Cistanche deserticola water extract, such as Figure 3eAs shown, it can be seen from the results that as the concentration of grape seed extract + wine-roasted Cistanche deserticola water extract increases, the cell survival rate increases, indicating that the sample promotes cell growth, so it can be considered as an eye care solution.

[0095] Formula 6: Steamed Cistanche deserticola water extract, such as Figure 3f As shown in the results, it can be seen that the steamed Cistanche deserticola water extract promotes cell growth. As the concentration increases, the cytotoxicity does not increase significantly, and the survival rate is higher than that of blank cells, indicating that the steamed Cistanche deserticola water extract promotes cell proliferation. Therefore, it can be used as an eye care solution.

[0096] 3.3 Cell scratch assay

[0097] 3.3.1 RCFBF cells

[0098] Formula 1: Grape seed extract, such as Figure 4a As shown, from the perspective of cell scratch repair, grape seed extract has no effect on cell scratch repair, and when the sample concentration is too high, it will cause cell death.

[0099] Formula 2: Grape seed extract: Vitamin C, such as Figure 4b As shown, it can be seen from the results that grape seed extract + vitamin C has no effect on cell repair, but the addition of vitamin C alleviates the cytotoxicity of grape seed extract to a certain extent.

[0100] Formula 3: Wine-roasted Cistanche deserticola water extract, such as Figure 4c As shown, the results do not show the repair effect of wine-roasted Cistanche deserticola water extract on cell scratches. The sugar component content in the Cistanche deserticola extract is relatively high, so the Cistanche deserticola extract is easily contaminated with bacteria.

[0101] Formula 4: Fresh Cistanche deserticola polysaccharide, such as Figure 4d As shown in the results, Cistanche deserticola polysaccharide has a certain repair effect on cell scratches, but the bacterial contamination of Cistanche deserticola polysaccharide is more serious than that of the extract. If used as an eye care solution, the antibacterial effect should be considered.

[0102] Formula 5: Grape seed extract: wine roasted Cistanche deserticola water extract, such as Figure 4e As shown, it can be seen from the results that the effect of grape seed extract + wine-roasted Cistanche deserticola water extract on cell scratch repair cannot be obtained, and the sample is contaminated with bacteria.

[0103] Formula 6: Steamed Cistanche deserticola water extract, such as Figure 4f As shown, the steamed Cistanche deserticola water extract sample was affected by bacteria and could not produce a scratch repair effect.

[0104] 3.3.2 RCFC cells

[0105] Formula 1: Grape seed extract, such as Figure 5a As shown, it can be seen from the cell pictures that the grape seed extract sample has no effect on cell scratch repair, but the sample is not contaminated with bacteria, indicating that the sample has a certain antibacterial effect.

[0106] Formula 2: Grape seed extract: Vitamin C, such as Figure 5b As shown in the results, it can be seen that the grape seed extract + vitamin C sample has no repair effect on cell scratches, and there is no obvious change in the cell scratches after 24h and 48h of culture.

[0107] Formula 3: Wine-roasted Cistanche deserticola water extract, such as Figure 5c As shown in the results, it can be seen that the wine-roasted Cistanche deserticola water extract has a certain repair effect on cell scratches, but the sample is easily contaminated with bacteria, so the antibacterial problem should be considered if it is used as a sample.

[0108] Formula 4: Fresh Cistanche deserticola polysaccharide, such as Figure 5d As shown in the results, it can be seen that Cistanche deserticola polysaccharide has a good repairing effect on cell scratches, and the scratch repair effect is better at a higher concentration. After 24 hours of culture, the scratches have a certain repair effect, but at 48 hours, the cells are contaminated with bacteria. Therefore, if used as an eye care solution, antibacterial properties need to be considered.

[0109] Formula 5: Grape seed extract: wine roasted Cistanche deserticola water extract, such as Figure 5e As shown, it can be seen from the results that grape seed extract + wine-roasted Cistanche deserticola water extract has no repair effect on cell scratches, but the sample is not contaminated with bacteria, so grape seed extract has an antibacterial effect, but it also weakens the repair effect of wine-roasted Cistanche deserticola water extract on cells.

[0110] Formula 6: Steamed Cistanche deserticola water extract, such as Figure 5f As shown in the results, it can be seen that the steamed Cistanche deserticola water extract has a certain effect on cell scratch repair, but the effect is not obvious, and the sample is contaminated with bacteria after 48 hours.

[0111] 4 Research Summary

[0112] From the experimental results, it can be seen that among the six formulas, the most toxic one is formula one. However, the results show that the toxicity of grape seed extract is significantly reduced at low concentrations, and the color of low-concentration grape seed extract is lighter. Therefore, grape seed extract can be used as an eye care solution at low concentrations.

[0113] Formula 2: The toxicity of the grape seed extract + vitamin C formula is similar to that of formula 1, but the cell survival rate of this sample at low concentration is higher than that of formula 1 at the same concentration, indicating that the toxicity of the formula is reduced after the addition of vitamin C. However, at high concentrations, the cytotoxicity is enhanced. Therefore, attention should be paid to the formula concentration when this formula is used as an eye care solution.

[0114] Formula 3 and Formula 6 are respectively: wine-roasted Cistanche deserticola water extract and steamed Cistanche deserticola water extract. From the results, it can be seen that the cytotoxicity trends of Formula 3 and Formula 6 are consistent. When the concentration is above 2 mg / ml, it will be toxic to cells. Below this concentration, the cell toxicity is relatively small, especially when the sample concentration is 500 and 1000 μg / ml, the cell survival rate is the highest, which is higher than the blank control and low concentration, indicating that the extract at this concentration has a promoting effect on cell growth. Therefore, this formula is suitable for use as an eye care solution.

[0115] The experimental results also show that the cytotoxicity trends of Cistanche deserticola samples prepared by different processing methods are the same. However, it was found during the experiment that this formula is very easy to be contaminated with bacteria due to the high content of sugar components. If it is used as an eye care solution, antibacterial issues should be considered.

[0116] Formula 4: Fresh Cistanche polysaccharide. The experimental results show that the toxicity of Cistanche polysaccharide is relatively low. When the sample concentration is above 3 mg / ml, the cell survival rate is lower than that of the blank control, and the survival rate is 93%. Therefore, the sample has relatively low toxicity, so this formula is suitable for use as an eye care solution. However, the experimental process found that this formula will cause turbidity in the solution during high concentration dissolution, so pay attention to the formula problem when using this formula as an eye care solution. In addition, due to the high content of Cistanche polysaccharide, this formula is very easy to be contaminated with bacteria. Therefore, pay attention to the antibacterial problem of the product when used as an eye care solution.

[0117] Formula 5: Grape seed extract: wine-roasted Cistanche deserticola water extract. The experimental results show that the cytotoxicity of this formula is weaker than that of formula 1, indicating that Cistanche deserticola extract can alleviate the cytotoxicity of grape seed extract and has a certain degree of relief on the bacterial infection of cells, so this formula can be considered for use.

[0118] In summary, when grape seed extract is used as an eye care solution, high concentrations should not be used, and adding a certain amount of vitamin C can help cell growth. Cistanche extract has a certain promoting effect on cell growth and can be used as an eye care solution, but the antibacterial problem should be considered. Cistanche polysaccharide has a greater promoting effect on cell growth, but the antibacterial problem needs to be considered when using this sample as an eye care solution. Formula five can be used as an eye care solution, and its cytotoxicity is low.

[0119] Example 1: Preparation of the first batch of samples, name: Cataract relief eye care solution.

[0120] Quantity: 100 bottles

[0121] Specification: 2ml / bottle

[0122] The formula and basis are as follows

[0123]

[0124] Raw and auxiliary materials

[0125] 0.1mg / ml grape seed extract, 0.5mg / ml wine-roasted Cistanche deserticola water extract, sodium chloride with purity ≥99.5%, Shihanquan stock solution.

[0126] Preparation Operation

[0127] Preparation of grape seed extract: Add distilled water to dried grape seeds at a solid-liquid ratio of 1:20, extract at 60°C for two hours, centrifuge and filter to remove insoluble matter, and dry the supernatant to obtain grape seed extract.

[0128] Preparation of wine-roasted Cistanche deserticola water extract: Cistanche deserticola is moistened with yellow wine and then dried, crushed and extracted with water, the solid-liquid ratio is 1:20, and the extraction is carried out at 80°C for 2 hours. After centrifugal filtration, the wine-roasted Cistanche deserticola water extract is obtained by spray drying.

[0129] Sample preparation: (1) Weigh 0.03 g of grape seed extract, 0.15 g of wine-roasted Cistanche deserticola extract, and 2.4 g of sodium chloride, add them to 300 ml of Shihan spring water, and fully dissolve them by ultrasonication; (2) Filter the prepared solution through a sterilization membrane for sterilization, and irradiate it under ultraviolet light for two hours; (3) Pipette 2 ml of the sterilized sample solution with a pipette tip, bottle it, and seal it.

[0130] Example 2: Preparation of the second batch of samples, name: Cataract relief eye care solution.

[0131] Quantity: 200 bottles; Specification: 2ml / bottle;

[0132] The formula and basis are as follows

[0133]

[0134] Raw and auxiliary materials

[0135] 0.1mg / ml grape seed extract, 0.5mg / ml wine-roasted Cistanche deserticola water extract, sodium chloride with purity ≥99.5%, Shihanquan stock solution.

[0136] Preparation Operation

[0137] Preparation of grape seed extract: Add distilled water to dried grape seeds at a solid-liquid ratio of 1:20, extract at 60°C for two hours, centrifuge and filter to remove insoluble matter, and dry the supernatant to obtain grape seed extract.

[0138] Preparation of wine-roasted Cistanche deserticola water extract: Cistanche deserticola is moistened with yellow wine and then dried, crushed and extracted with water, the solid-liquid ratio is 1:20, and the extraction is carried out at 80°C for 2 hours. After centrifugal filtration, the wine-roasted Cistanche deserticola water extract is obtained by spray drying.

[0139] Sample preparation: (1) Weigh 0.05 g of grape seed extract, 0.25 g of wine-roasted Cistanche deserticola water extract, and 4 g of sodium chloride, add them to 500 ml of Shihan spring water, and fully dissolve them by ultrasonication; (2) Filter the prepared solution through a sterilization membrane for sterilization and irradiate it under ultraviolet light for two hours; (3) Pipette 2 ml of the sterilized sample solution with a pipette tip, bottle it, and seal it.

[0140] Example 3: Preparation of the third batch of samples, name: Cataract-relieving eye care solution.

[0141] Quantity: 200 bottles; Specification: 2ml / bottle;

[0142] The formula and basis are as follows

[0143]

[0144] Raw and auxiliary materials

[0145] 0.1mg / ml grape seed extract, 0.5mg / ml wine-roasted Cistanche deserticola water extract, sodium chloride with purity ≥99.5%, Shihanquan stock solution.

[0146] Preparation Operation

[0147] Preparation of grape seed extract: Add distilled water to dried grape seeds at a solid-liquid ratio of 1:20, extract at 60°C for two hours, centrifuge and filter to remove insoluble matter, and dry the supernatant to obtain grape seed extract.

[0148] Preparation of wine-roasted Cistanche deserticola water extract: Cistanche deserticola is moistened with yellow wine and then dried, crushed and extracted with water, the solid-liquid ratio is 1:20, and the extraction is carried out at 80°C for 2 hours. After centrifugal filtration, the wine-roasted Cistanche deserticola water extract is obtained by spray drying.

[0149] Sample preparation: (1) Weigh 0.025g of grape seed extract, 0.125g of wine-roasted Cistanche deserticola water extract and 2.0g of sodium chloride respectively, add them to 250ml of Shihan spring water, and fully dissolve them by ultrasonication; (2) Filter the prepared solution with a sterilization membrane for sterilization and irradiate it under ultraviolet light for two hours; (3) Pipette 2ml of the sterilized sample solution with a pipette tip, bottle it, and seal it.

[0150] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant extract for preparing an eye care solution for relieving cataracts, characterized in that: The invention comprises the following raw materials in parts by weight: 0.025-0.05 parts of grape seed extract, 0.125-0.25 parts of long-moxibustion Cistanche deserticola extract, 2-4 parts of sodium chloride and 250-500 parts of Shihan spring water.

2. The cataract-relieving eye care solution prepared from a plant extract according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 0.03 parts of grape seed extract, 0.15 parts of long-moxibustion Cistanche deserticola extract, 2.4 parts of sodium chloride and 300 parts of Shihan spring water.

3. The cataract-relieving eye care solution prepared from a plant extract according to claim 2, characterized in that: The following preparation steps are also included: S1. Weigh grape seed extract, wine-roasted Cistanche deserticola water extract and sodium chloride in proportion, add them into Shihan spring water, and fully dissolve them by ultrasonication; S2. Filter the prepared solution through a sterilization membrane and sterilize it under ultraviolet light for two hours; S3. Use a pipette to draw 2 ml of the sterilized solution, bottle it, and seal it.

4. The cataract-relieving eye care solution prepared from a plant extract according to claim 1, characterized in that: The preparation method of the grape seed extract is as follows: adding distilled water to dried grape seeds at a solid-liquid ratio of 1:20, extracting at 60° C. for two hours, centrifuging and filtering to remove insoluble matter, and drying the supernatant to obtain the grape seed extract.

5. The cataract-relieving eye care solution prepared from a plant extract according to claim 3, characterized in that: The preparation method of the wine-roasted Cistanche deserticola water extract is as follows: the Cistanche deserticola is moistened with yellow wine and then dried, crushed and then extracted with water, the solid-liquid ratio is 1:20, the extraction is carried out at 80°C for 2 hours, and after centrifugal filtration, the wine-roasted Cistanche deserticola water extract is obtained by spray drying.

6. The cataract-relieving eye care solution prepared from a plant extract according to claim 1, characterized in that: The rare spring water is natural soda water.

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