Pinellia tuber fermented mass and preparation method thereof

Abstract

The invention provides rhizoma pinellinae praeparata fermented and a preparation method thereof.According to the method, raw rhizoma pinellinae praeparata cultivated through a hydrogen peroxide silver ion compound solvent is adopted to inhibit toxin growth in the medicinal material cultivation period, then rhizoma pinellinae praeparata and rhizoma pinellinae fermented are prepared in sequence through the raw rhizoma pinellinae praeparata, and a drying technology involved in the process of preparing the rhizoma pinellinae praeparata and the rhizoma pinellinae fermented is used for preparing rhizoma pinellinae praeparata. High-pressure mercury lamp ultraviolet rays are adopted for irradiation to control toxins in the production process, in addition, in the fermented pinellia preparation process, litsea cubeba oil is dropwise added, the purpose of controlling the toxins is further achieved, and understandable, by means of the method, on the premise that the efficacy of the fermented pinellia is not affected, the production efficiency of the fermented pinellia is improved, and the production cost is reduced. The content of aflatoxin in the fermented pinellia is effectively controlled.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a kind of pinellia tuber and a preparation method thereof. Background Art

[0002] Pinellia tuber is a processed product made by fermenting French pinellia tuber, adzuki bean, bitter almond, fresh Artemisia annua, fresh Polygonum hydropiper, fresh Xanthium sibiricum and flour.

[0003] It should be noted that Chinese medicine may be contaminated with mycotoxins during the production, harvesting, processing, transportation, and storage processes due to the combined effects of its own properties (internal factors) and external factors (external factors). Internal factors refer to the nutrients in the Chinese medicinal materials themselves (such as water, protein, sugars, oils, etc.) that can provide the necessary substances for the growth of mold. External factors refer to improper human handling, which provides the necessary production environment for mold, thereby increasing the probability of mycotoxin contamination. This phenomenon is particularly prominent in the hot and humid areas in the south.

[0004] Among them, taking aflatoxin as an example, the four most common and extremely harmful ones are aflatoxin B1, B2, G1, and G2. B1 is the most toxic, 10 times that of potassium cyanide and 68 times that of arsenic. The median lethal dose of aflatoxin B1 is 0.36 mg / kg. The so-called median lethal dose refers to the drug dose required to cause the death of at least half of the experimental subjects. Therefore, how to control the content of aflatoxin in Pinellia ternata is particularly important. Summary of the invention

[0005] Based on this, an embodiment of the present invention provides a Pinellia tuber and a preparation method thereof, aiming to control the content of aflatoxin in the Pinellia tuber without affecting the efficacy of the Pinellia tuber.

[0006] A first aspect of the embodiments of the present invention provides a method for preparing Pinellia tuber, the method comprising:

[0007] Take raw pinellia, separate them according to the raw pinellia specifications, soak them in clean water respectively, change the water every day, and drain the water twice during the water change process, wherein the raw pinellia is regularly sprayed with hydrogen peroxide silver ion composite solvent on the leaf surface of the raw pinellia during the cultivation stage;

[0008] Add alum to the soaked raw pinellia, stir evenly, pickle for a preset time, add clean water to soak for a second time, and dry after soaking, wherein 5 kg of alum is used for every 100 kg of raw pinellia;

[0009] Pour the dried sample into a container, stir evenly with sapodilla, dried ginger, mint, tangerine peel, liquorice, alum, saltpeter, add boiling water to brew and stir, control the juice to exceed the medicine surface by 14cm-16cm, seal, soak in a cool place indoors, take out, wash, cut into thick slices, dry, and obtain French pinellia, wherein, for every 100kg of raw pinellia, use 3kg of sapodilla, dried ginger, mint, tangerine peel, liquorice, 12.5kg of alum, and 5kg of saltpeter;

[0010] Grind pinellia tuber, adzuki bean and bitter almond into fine powder, mix evenly with flour, add decoction of fresh artemisia annua, fresh polygonum hydropiper and fresh Xanthium sibiricum, and drop litsea cubeba oil, heat and stir evenly, ferment, shape and dry to obtain pinellia tuber. During the above drying processes, ultraviolet light from a high-pressure mercury lamp is used for irradiation.

[0011] Furthermore, the method comprises grinding pinellia tuber, adzuki bean and bitter almond into fine powder, mixing the powder evenly with flour, adding decoction of fresh artemisia annua, fresh polygonum hydropiper and fresh xanthium sibiricum, dripping litsea cubeba oil, heating and stirring evenly, fermenting, shaping and drying to obtain pinellia tuber koji, which comprises the following steps:

[0012] Grind pinellia tuber, adzuki bean and bitter almond into fine powder, mix evenly with flour, add decoction of fresh Artemisia annua, fresh Polygonum hydropiper and fresh Xanthium sibiricum;

[0013] Divide the Litsea cubeba oil into three equal parts and add them dropwise into the above mixture three times;

[0014] The process is heated and stirred evenly, fermented, formed, and dried to obtain the pinellia tuber.

[0015] Furthermore, during the process of irradiating with ultraviolet light from a high-pressure mercury lamp, the temperature of the surface of the medicinal material irradiated by the ultraviolet light from the high-pressure mercury lamp is controlled to be above 268°C.

[0016] Furthermore, the drying process is as follows: first, the medicinal materials to be dried are placed in a drying box, and a high-pressure mercury lamp ultraviolet device is also installed in the drying box. While controlling the operation of the drying box, the high-pressure mercury lamp ultraviolet device is controlled to operate intermittently, wherein the operating time of the high-pressure mercury lamp ultraviolet device is controlled to account for 5% to 10% of the total drying time.

[0017] Furthermore, the drying temperature of the drying oven is set to 40° C. to 50° C., and the drying time of the drying oven is set to 4 h to 6 h.

[0018] Furthermore, the total number of operations of the high-pressure mercury lamp ultraviolet device is determined according to the drying time of the drying box, wherein the total number of operations of the high-pressure mercury lamp ultraviolet device is 1 to 2 times per drying hour, and the time interval between two operations of the high-pressure mercury lamp ultraviolet device is gradually extended.

[0019] Furthermore, in the step of dividing the litsea cubeba oil into three equal parts and dropping them into the above mixture three times, the mass ratio of the added litsea cubeba oil to the fine powder of pinellia tuber, adzuki bean and bitter almond is 1:100.

[0020] Furthermore, in the step of heating and stirring uniformly, the heating temperature is 40° C. to 50° C., and the stirring rate is 20 r / min to 80 r / min.

[0021] Furthermore, in the fermentation step, the fermentation temperature is 30°C to 35°C, and the fermentation days are 4 to 5 days.

[0022] A second aspect of the embodiments of the present invention provides a kind of Pinellia tuber, which is prepared by the above-mentioned Pinellia tuber preparation method.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The method comprises the following steps: taking raw pinellia, separating the raw pinellia according to the raw pinellia specifications, soaking them in clean water respectively, changing the water every day, and draining the water twice during the water changing process, wherein the raw pinellia is regularly sprayed with a hydrogen peroxide silver ion composite solvent on the leaf surface of the raw pinellia during the cultivation stage; adding alum to the soaked raw pinellia, stirring evenly, adding clean water to soak for a second time after pickling for a preset time, and drying after soaking, wherein 5 kg of alum is used for every 100 kg of raw pinellia; pouring the dried sample into a container, stirring evenly with soap beans, dried ginger, mint, tangerine peel, licorice, alum, and saltpeter, adding boiling water to brew and stir, controlling the medicine juice to exceed the medicine surface by 14 cm to 16 cm, sealing, and soaking in a cool place indoors until the mouth is dry. When a slight numbness is felt on the tongue, take it out, wash it, cut it into thick slices, and dry it to obtain the French pinellia, wherein for every 100 kg of raw pinellia, use 3 kg each of soap beans, dried ginger, mint, tangerine peel, and licorice, 12.5 kg of alum, and 5 kg of saltpeter; grind the French pinellia, red beans, and bitter almonds into fine powder, mix them evenly with flour, add the decoction of fresh Artemisia annua, fresh Polygonum hydropiper, and fresh Xanthium sibiricum, and drop into Litsea cubeba oil, heat and stir evenly, ferment, shape, and dry to obtain Pinellia ternata. In the above-mentioned drying processes, high-pressure mercury lamp ultraviolet light is used for irradiation. Specifically, since aflatoxin is inhibited and removed during the whole process of the raw material raw pinellia and the preparation of Pinellia ternata, the purpose of controlling the aflatoxin content in Pinellia ternata without affecting the efficacy of Pinellia ternata is achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the relationship between the extract content of Pinellia tuber and the fermentation days at different temperatures;

[0026] Figure 2 The toxin test spectrum of the Pinellia tuber powder prepared in Example 1 of the present invention;

[0027] Figure 3 A parameter table corresponding to the toxin test spectrum of the Pinellia tuber powder prepared in Example 1 of the present invention;

[0028] Figure 4 The toxin test spectrum of Pinellia tuber powder prepared by the method of Example 1 of the present invention but without adding Litsea cubeba oil;

[0029] Figure 5 This is a parameter table of the toxin test spectrum of Pinellia tuber powder prepared by the method of Example 1 of the present invention but without adding Litsea cubeba oil;

[0030] Figure 6 The toxin test spectrum of Pinellia tuber powder prepared by the existing method;

[0031] Figure 7 This is a parameter table of the toxin test spectrum of Pinellia tuber powder prepared by the existing method.

[0032] The following specific implementation manner will be further described in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0033] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0035] The preparation method of the Pinellia tuber in the embodiment of the present invention specifically comprises the following steps:

[0036] Step S01, taking raw pinellia, separating them according to their specifications, soaking them in clean water respectively, changing the water every day, and draining the water twice during the water change process. During the cultivation stage, the raw pinellia is regularly sprayed with a hydrogen peroxide silver ion composite solvent on the leaves of the raw pinellia.

[0037] Specifically, take raw pinellia, separate large and small ones, add clean water to soak for 7 to 10 days, change the water 2 to 3 times a day, and drain the water twice in the middle. During the cultivation stage, the raw pinellia is regularly sprayed with a hydrogen peroxide silver ion composite solvent on the leaf surface of the raw pinellia. The hydrogen peroxide silver ion composite solvent can decompose aflatoxin produced during the growth of the raw pinellia, and effectively inhibit the diffusion of aflatoxin produced during the growth of the raw pinellia. In this embodiment, the hydrogen peroxide silver ion composite solvent is a hydrogen peroxide silver ion composite solvent produced by Oxytech. The specific method of use is to spray the hydrogen peroxide silver ion composite solvent on the leaf surface of the raw pinellia for disinfection for 20 minutes to 30 minutes, and then rinse the leaf surface of the raw pinellia with clean water.

[0038] Step S02, adding alum to the soaked raw pinellia, stirring evenly, and after marinating for a preset time, adding clean water to soak for a second time, and drying after soaking, wherein 5 kg of alum is used for every 100 kg of raw pinellia.

[0039] Specifically, alum is added to the soaked raw pinellia tuber, and the mixture is stirred evenly. After being pickled for 24 hours, clean water is added for secondary soaking, and the mixture is dried after being soaked for 7 to 10 days. It should be noted that the drying process is as follows: first, the medicinal material to be dried is placed in a drying oven, and a high-pressure mercury lamp ultraviolet device is also installed in the drying oven. While controlling the operation of the drying oven, the high-pressure mercury lamp ultraviolet device is controlled to operate intermittently, wherein the operating time of the high-pressure mercury lamp ultraviolet device accounts for 5% to 10% of the total drying time. In addition, the drying temperature of the drying oven is set to 40° C. to 50° C., so that the medicinal material is dried in a low temperature environment, and the temperature of the high-pressure mercury lamp ultraviolet light irradiating the surface of the medicinal material is controlled to be 268° C. ℃ or above, while removing aflatoxins, retaining part of the moisture of the medicinal materials, the drying time of the drying oven is set to 4h~6h, in order to make the drying of the medicinal materials and the removal of aflatoxins better, a multi-layer mesh structure is evenly arranged in the drying oven, and the aperture size of the mesh is based on the medicinal materials not leaking out, wherein each layer of the mesh structure includes two mesh plates buckled up and down, and the two mesh plates sandwich the flat medicinal materials in the middle, and at the same time, each layer of the mesh structure can be connected to the inner wall of the drying oven through a rotating shaft, and driven by the rotating shaft, each layer of the mesh structure rotates at a uniform speed in the drying oven, because high-pressure mercury lamp ultraviolet devices are evenly laid around the drying oven, so that the surface of the medicinal materials is evenly irradiated.

[0040] In this embodiment, the total number of operations of the high-pressure mercury lamp ultraviolet device is determined according to the drying time of the drying oven, wherein the total number of operations of the high-pressure mercury lamp ultraviolet device is 1 to 2 times per drying hour, and the time interval between two operations of the high-pressure mercury lamp ultraviolet device is gradually extended. It can be understood that if the drying time of the drying oven is set to 4 hours, the total number of operations of the high-pressure mercury lamp ultraviolet device is 4 to 8 times. According to the control of the operation time of the high-pressure mercury lamp ultraviolet device accounting for 5% to 10% of the total drying time, it can be obtained that the control operation time of the high-pressure mercury lamp ultraviolet device is 0.2h to 0.4h. It should be noted that due to the low temperature drying Drying, the water content of medicinal materials in the initial drying stage is high, and it is more likely to produce aflatoxin in a humid environment. Therefore, as the drying time increases, the time interval between the two high-pressure mercury lamp ultraviolet device operations is gradually extended, that is, the operation frequency of the high-pressure mercury lamp ultraviolet device in the initial drying stage is higher than that in the later drying stage. For example, when the drying time of the drying oven is set to 4h, the total number of operations of the high-pressure mercury lamp ultraviolet device is 5 times, and the operating time of the high-pressure mercury lamp ultraviolet device is controlled to be 0.2h, that is, 12min. Since the total number of operations of the high-pressure mercury lamp ultraviolet device is 5 times, then the time for each operation is 2.4min, and the first control When the high-pressure mercury lamp ultraviolet device is controlled to run for 15 minutes of drying, the high-pressure mercury lamp ultraviolet device is controlled to run for 2.4 minutes at this moment; when the high-pressure mercury lamp ultraviolet device is controlled to run for the second time, the high-pressure mercury lamp ultraviolet device is controlled to run for 30 minutes of drying, the high-pressure mercury lamp ultraviolet device is controlled to run for 2.4 minutes at this moment; when the high-pressure mercury lamp ultraviolet device is controlled to run for the third time, the high-pressure mercury lamp ultraviolet device is controlled to run for 1 hour of drying, the high-pressure mercury lamp ultraviolet device is controlled to run for 2.4 minutes at this moment; when the high-pressure mercury lamp ultraviolet device is controlled to run for the fourth time, the high-pressure mercury lamp ultraviolet device is controlled to run for 2 hours of drying, the high-pressure mercury lamp ultraviolet device is controlled to run for 2.4 minutes at this moment; and when the high-pressure mercury lamp ultraviolet device is controlled to run for the fifth time, the high-pressure mercury lamp ultraviolet device is controlled to run for 1 hour of drying. When drying for 4 hours, the high-pressure mercury lamp ultraviolet device is controlled to run for 2.4 minutes. It can be understood that the time interval between the first control of the high-pressure mercury lamp ultraviolet device and the second control of the high-pressure mercury lamp ultraviolet device is 15 minutes, the time interval between the second control of the high-pressure mercury lamp ultraviolet device and the third control of the high-pressure mercury lamp ultraviolet device is 30 minutes, the time interval between the third control of the high-pressure mercury lamp ultraviolet device and the fourth control of the high-pressure mercury lamp ultraviolet device is 1 hour, and the time interval between the fourth control of the high-pressure mercury lamp ultraviolet device and the fifth control of the high-pressure mercury lamp ultraviolet device is 2 hours, and the time intervals are gradually extended.

[0041] It should be noted that in the subsequent drying step, the above-mentioned drying method is also used for drying.

[0042] Step S03, pouring the dried sample into a container, mixing the dried sample with sapodilla, dried ginger, mint, tangerine peel, liquorice, alum, and saltpeter, adding boiling water to brew and stir, controlling the medicine juice to exceed the medicine surface by 14cm to 16cm, sealing, and soaking in a cool place indoors until a slight numbness in the tongue is tasted, taking it out, washing, cutting into thick slices, and drying to obtain French pinellia, wherein, for every 100kg of raw pinellia, 3kg each of sapodilla, dried ginger, mint, tangerine peel, and liquorice, 12.5kg of alum, and 5kg of saltpeter are used.

[0043] Exemplarily, the medicine juice is controlled to exceed the medicine surface by 14 cm, 14.5 cm, 15 cm, 15.5 cm or 16 cm, but is not limited thereto.

[0044] Step S04, grind the pinellia tuber, adzuki bean and bitter almond into fine powder, mix them evenly with flour, add the decoction of fresh Artemisia annua, fresh Polygonum hydropiper and fresh Xanthium sibiricum, and drop litsea cubeba oil, heat and stir evenly, ferment, shape and dry to obtain pinellia tuber.

[0045] Specifically, take Pinellia ternata, Adzuki bean and bitter almond and grind them into fine powder, mix them evenly with flour, add decoction of fresh Artemisia annua, fresh Polygonum hydropiper and fresh Xanthium sibiricum; divide Litsea cubeba oil into three equal parts, and drip them into the above mixture three times, wherein the mass ratio of Litsea cubeba oil to the ground powder of Pinellia ternata, Adzuki bean and bitter almond is 1:100; heat and stir evenly, ferment, shape and dry to obtain Pinellia ternata koji, wherein in the step of heating and stirring evenly, the heating temperature is 40°C to 50°C, and illustratively, the heating temperature is 40°C , 42°C, 44°C, 46°C, 48°C or 50°C, but not limited thereto, the stirring rate is 20r / min-80r / min, exemplarily, the stirring rate is 20r / min, 40r / min, 60r / min or 80r / min, but not limited thereto, in the fermentation step, the fermentation temperature is 30°C-35°C, exemplarily, the fermentation temperature is 30°C, 31°C, 32°C or 33°C, 34°C or 35°C, but not limited thereto, the fermentation days are 4-5 days, please refer to Figure 1, is a schematic diagram of the relationship between the extract content of Pinellia ternata and the number of fermentation days at different temperatures. It should be noted that at 25℃, the growth of Pinellia ternata Aspergillus is slow, and green mold spots appear; at 40℃, the mold also grows slowly, which is not conducive to the fermentation of Pinellia ternata; there is little difference between the fermentation of Pinellia ternata at 30℃ and 32℃, which is normal fermentation; the fermentation speed at 35℃ is faster than that at 30℃ and 32℃, and the mold grows vigorously. On the 6th day, a smell similar to moldy tofu appeared. It is recommended that the fermentation time at 35℃ should not exceed 5 days. The fermentation temperature was investigated, and the fermentation temperature was set at 25℃, 30℃, 32℃, 35℃, and 40℃; the powder was coarse powder. Samples were taken at 3, 4, 5, 6, and 7 days of fermentation, respectively. Combined with the results of properties and extract content, the suitable fermentation temperature of Pinellia ternata is 30℃~35℃. Please refer to Table 1 for the determination results of water-soluble extracts of Pinellia ternata.

[0046] Table 1. Results of determination of water-soluble extracts from Pinellia tuber

[0047]

[0048]

[0049] In summary, the method for preparing Pinellia tuber provided by the embodiment of the present invention comprises the following steps: taking raw Pinellia tuber, separating the raw Pinellia tuber according to the specifications of the raw Pinellia tuber, respectively adding clean water to soak, changing the water every day, draining the water twice during the water changing process, wherein the raw Pinellia tuber is regularly sprayed with a hydrogen peroxide silver ion composite solvent on the leaf surface of the raw Pinellia tuber during the cultivation stage; adding alum to the soaked raw Pinellia tuber, stirring evenly, adding clean water to soak for a second time after pickling for a preset time, and drying after soaking, wherein 5 kg of alum is used for every 100 kg of raw Pinellia tuber; pouring the dried sample into a container, stirring evenly with soap beans, dried ginger, mint, tangerine peel, licorice, alum, and saltpeter, adding boiling water to brew and stir, controlling the juice to exceed the medicine surface by 14 cm to 16 cm, and sealing , soak in a cool place indoors until the tongue feels slightly numb, take it out, wash it, cut it into thick slices, and dry it to obtain French pinellia, wherein, for every 100kg of raw pinellia, use 3kg each of soap beans, dried ginger, mint, tangerine peel, and licorice, 12.5kg of alum, and 5kg of saltpeter, and irradiate with ultraviolet light from a high-pressure mercury lamp during the drying process; grind French pinellia, red beans, and bitter almonds into fine powder, mix them evenly with flour, add decoction of fresh Artemisia annua, fresh Polygonum aviculare, and fresh Xanthium sibiricum, and drop into Litsea cubeba oil, heat and stir evenly, ferment, shape, and dry to obtain Pinellia ternata. Specifically, since aflatoxin is inhibited and removed during the whole process of raw material raw pinellia and preparation of Pinellia ternata, the purpose of controlling the content of aflatoxin in Pinellia ternata without affecting the efficacy of Pinellia ternata is achieved.

[0050] On the other hand, the present invention also provides a kind of pinellia tuber, which is prepared by adopting the preparation method of pinellia tuber mentioned above.

[0051] In order to facilitate understanding of the present invention, several embodiments of the present invention are provided below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0052] Example 1

[0053] In Example 1 of the present invention, raw pinellia is taken, separated by large and small sizes, and soaked in clean water for 7 days. The water is changed twice a day, and the water is drained twice in the middle. During the cultivation stage, the raw pinellia is regularly sprayed with a hydrogen peroxide silver ion composite solvent on the leaf surface of the raw pinellia. Specifically, the hydrogen peroxide silver ion composite solvent is a hydrogen peroxide silver ion composite solvent produced by Oxytech. The specific method of use is to spray the hydrogen peroxide silver ion composite solvent on the leaf surface of the raw pinellia, disinfect it for 20 minutes, and then rinse the leaf surface of the raw pinellia with clean water.

[0054] Add alum to the soaked raw pinellia, 5kg of alum for every 100kg of raw pinellia, stir evenly, pickle for 24h, add clean water for secondary soaking, and dry after soaking for 7 days. It should be noted that the drying process is as follows: first, put the medicinal materials to be dried into a drying oven, which is also equipped with a high-pressure mercury lamp ultraviolet device. While controlling the operation of the drying oven, the high-pressure mercury lamp ultraviolet device is controlled to operate intermittently, wherein the operating time of the high-pressure mercury lamp ultraviolet device accounts for 5% of the total drying time. In addition, the drying temperature of the drying oven is set to 48°C, so that the medicinal materials are dried in a low temperature environment, and the high pressure mercury lamp ultraviolet device is controlled. The temperature of the surface of the medicinal materials irradiated by ultraviolet light from a mercury pressure lamp is 270°C. While removing aflatoxin, part of the moisture of the medicinal materials is retained. The drying time of the drying oven is set to 4 hours. In order to achieve better drying of the medicinal materials and removal of aflatoxin, a multi-layer mesh structure is evenly arranged in the drying oven. The aperture size of the mesh is based on the fact that the medicinal materials do not leak out. Each layer of the mesh structure includes two mesh plates that are buckled up and down. The two mesh plates sandwich the flat medicinal materials in the middle. At the same time, each layer of the mesh structure can be connected to the inner wall of the drying oven through a rotating shaft. Driven by the rotating shaft, each layer of the mesh structure rotates at a uniform speed in the drying oven.

[0055] In this embodiment, the total number of operations of the high-pressure mercury lamp ultraviolet device is determined according to the drying time of the drying oven, wherein the total number of operations of the high-pressure mercury lamp ultraviolet device is 5 times, and the time interval between two operations of the high-pressure mercury lamp ultraviolet device is gradually extended, and the operation time of the high-pressure mercury lamp ultraviolet device is controlled to be 0.2h. Specifically, when the first time the high-pressure mercury lamp ultraviolet device is controlled to operate for 15min of drying, the high-pressure mercury lamp ultraviolet device is controlled to operate for 2.4min at this moment; when the second time the high-pressure mercury lamp ultraviolet device is controlled to operate for 30min of drying, the high-pressure mercury lamp ultraviolet device is controlled to operate for 2.4min at this moment; when the third time the high-pressure mercury lamp ultraviolet device is controlled to operate for 1h of drying, the high-pressure mercury lamp ultraviolet device is controlled to operate for 2.4min at this moment; and when the fourth time the high-pressure mercury lamp ultraviolet device is controlled to operate for 1h of drying, the high-pressure mercury lamp ultraviolet device is controlled to operate for 2.4min at this moment. When the operating time is drying for 2 hours, the high-pressure mercury lamp ultraviolet device is controlled to run for 2.4 minutes. When the fifth operating time is drying for 4 hours, the high-pressure mercury lamp ultraviolet device is controlled to run for 2.4 minutes. It can be understood that the time interval between the first control of the high-pressure mercury lamp ultraviolet device and the second control of the high-pressure mercury lamp ultraviolet device is 15 minutes, the time interval between the second control of the high-pressure mercury lamp ultraviolet device and the third control of the high-pressure mercury lamp ultraviolet device is 30 minutes, the time interval between the third control of the high-pressure mercury lamp ultraviolet device and the fourth control of the high-pressure mercury lamp ultraviolet device is 1 hour, and the time interval between the fourth control of the high-pressure mercury lamp ultraviolet device and the fifth control of the high-pressure mercury lamp ultraviolet device is 2 hours, and the time intervals are gradually extended.

[0056] It should be noted that in the subsequent drying step, the above-mentioned drying method is also used for drying.

[0057] Pour the above dried sample into a container, mix it evenly with soap beans, dried ginger, mint, tangerine peel, licorice, alum, and saltpeter, add boiling water to brew and stir, control the juice to exceed the medicine surface by 14 cm, seal, and soak in a cool place indoors until there is a slight numbness in the tongue, take it out, wash, cut into thick slices, and dry to obtain French pinellia, wherein, for every 100 kg of raw pinellia, use 3 kg of soap beans, dried ginger, mint, tangerine peel, and licorice, 12.5 kg of alum, and 5 kg of saltpeter.

[0058] Specifically, take Pinellia ternata, Adzuki bean and bitter almond and grind them into fine powder, mix them evenly with flour, add decoction of fresh Artemisia annua, fresh Polygonum hydropiper and fresh Xanthium sibiricum; divide Litsea cubeba oil into three equal parts, and drip them into the above mixture three times, wherein the mass ratio of Litsea cubeba oil to the fine powder of Pinellia ternata, Adzuki bean and bitter almond is 1:100; heat and stir evenly, ferment, shape and dry to obtain Pinellia ternata koji, wherein in the step of heating and stirring evenly, the heating temperature is 48°C and the stirring rate is 80r / min, and in the step of fermentation, the fermentation temperature is 35°C and the fermentation days are 4 days.

[0059] Example 2

[0060] The difference between the preparation method of Pinellia tuber in Example 2 of the present invention and that in Example 1 is that the operating time of the high-pressure mercury lamp ultraviolet device is controlled to account for 8% of the total drying time, that is, when the drying time of the drying oven is set to 4h, the operating time of the high-pressure mercury lamp ultraviolet device is controlled to be 0.32h.

[0061] Example 3

[0062] The difference between the preparation method of Pinellia tuber in Example 3 of the present invention and that in Example 1 is that the operating time of the high-pressure mercury lamp ultraviolet device is controlled to account for 10% of the total drying time, that is, when the drying time of the drying oven is set to 4 hours, the operating time of the high-pressure mercury lamp ultraviolet device is controlled to be 0.4 hours.

[0063] Example 4

[0064] The difference between the preparation method of Pinellia tuber in Example 4 of the present invention and that in Example 1 is that the drying temperature of the drying oven is set to 40°C, the drying time of the drying oven is set to 6h, the total number of operations of the high-pressure mercury lamp ultraviolet device is 7 times, and the time interval between the two operations of the high-pressure mercury lamp ultraviolet device is gradually extended. Specifically, the operation time of the high-pressure mercury lamp ultraviolet device is controlled to be 0.3h, the first time the operation time of the high-pressure mercury lamp ultraviolet device is controlled to be dried for 5min, at this moment, the high-pressure mercury lamp ultraviolet device is controlled to be operated for 2.57min, the second time the operation time of the high-pressure mercury lamp ultraviolet device is controlled to be dried for 10min, at this moment, the high-pressure mercury lamp ultraviolet device is controlled to be operated for 2.57min, and the third time the operation time of the high-pressure mercury lamp ultraviolet device is controlled to be operated for The first time the high-pressure mercury lamp ultraviolet device was controlled to run for 2.57 minutes, the fourth time the high-pressure mercury lamp ultraviolet device was controlled to run for 2 hours, the fifth time the high-pressure mercury lamp ultraviolet device was controlled to run for 80 minutes, the sixth time the high-pressure mercury lamp ultraviolet device was controlled to run for 160 minutes, the seventh time the high-pressure mercury lamp ultraviolet device was controlled to run for 320 minutes, the high-pressure mercury lamp ultraviolet device was controlled to run for 2.57 minutes, and the time intervals were gradually extended.

[0065] Example 5

[0066] The difference between the preparation method of Pinellia tuber in Example 5 of the present invention and that in Example 4 is that the drying temperature of the drying oven is set to 50°C.

[0067] Example 6

[0068] The difference between the preparation method of Pinellia tuber in Example 6 of the present invention and that in Example 4 is that the drying temperature of the drying oven is set to 45°C.

[0069] Comparative Example 1

[0070] The difference between the preparation method of Pinellia tuber in this comparative example 1 and that in Example 1 is that no litsea cubeba oil is added during the preparation process.

[0071] Comparative Example 2

[0072] The difference between the preparation method of Pinellia tuber in Comparative Example 2 and that in Example 1 is that, during the cultivation stage of raw Pinellia tuber, the hydrogen peroxide silver ion complex solvent is not regularly sprayed on the leaf surface of raw Pinellia tuber.

[0073] Comparative Example 3

[0074] The difference between the preparation method of Pinellia ternata in Comparative Example 3 and that in Example 1 is that the drying process involved in the preparation of Pinellia ternata and Pinellia ternata does not use high-pressure mercury lamp ultraviolet irradiation.

[0075] Comparative Example 4

[0076] The difference between the preparation method of Pinellia tuber in this comparative example 4 and that in Example 1 is that during the irradiation with ultraviolet light from a high-pressure mercury lamp, the temperature of the surface of the medicinal material irradiated by the ultraviolet light from the high-pressure mercury lamp is controlled to be 265°C.

[0077] Comparative Example 5

[0078] This comparative example 5 is the Pinellia tuber powder prepared by the existing method.

[0079] Take the Pinellia powder in the above Examples 1 to 6 and Comparative Examples 1 to 5 respectively, take about 5 g of the test sample powder (pass through a No. 2 sieve), accurately weigh, accurately add 50 mL of 70% methanol solution, vortex mix for 1 min, ultrasonically treat for 30 min, centrifuge at 5000 r / min for 5 min, accurately measure 10 mL of the supernatant, dilute to 20 mL with water, and vortex mix.

[0080] Furthermore, accurately pipette 3 mL and slowly pass it through a treated HLB column (specification: 3 mL, 60 mg. Elute with 3 mL of methanol and water in turn) until an appropriate amount of air passes through, and collect the eluate; then elute with 3 mL of methanol, collect the eluate, combine the two eluates, slowly blow nitrogen at 40°C until nearly dry, add 50% acetonitrile solution to make the volume 1 mL, filter with a microporous filter membrane (0.22 μm), and take the filtrate to obtain the test solution.

[0081] Adopt the determination method, accurately take 5ul of each of the above series of mixed reference solutions, inject into high performance liquid chromatography-mass spectrometer, determine the peak area, and draw a standard curve with the peak area as the ordinate and the injection concentration as the abscissa. Accurately take 5ul of the above test solution, inject into high performance liquid chromatography-mass spectrometer, determine the peak area, read the total concentration of the test solution equivalent to aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, vomitoxin, fumonisin B1, fumonisin B2 and T-2 toxin from the standard curve, and calculate to obtain.

[0082] See also Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7 , Figure 2 This is the toxin test spectrum of the Pinellia tuber powder prepared in Example 1 of the present invention, Figure 3The parameter table corresponding to the toxin test spectrum of the Pinellia tuber powder prepared in Example 1 of the present invention is as follows: Figure 4 This is the toxin test spectrum of the Pinellia tuber powder prepared by the method of Example 1 of the present invention but without adding Litsea cubeba oil. Figure 5 This is a parameter table of the toxin test spectrum of the Pinellia tuber powder prepared by the method of Example 1 of the present invention but without adding Litsea cubeba oil. Figure 6 This is the toxin test spectrum of Pinellia tuber powder prepared by the existing method. Figure 7 The parameter table of the toxin test spectrum of the Banxiaqu powder prepared by the existing method shows that the toxins of the Banxiaqu powder in the prior art are relatively obvious and not well controlled. Figure 2 and Figure 4 The toxins in the samples were obviously controlled, but aflatoxin G1 still existed in the Pinellia tuber powder prepared without adding Litsea cubeba oil.

[0083] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0084] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for preparing Pinellia tuber, characterized in that: The method comprises: Take raw pinellia, separate them according to the raw pinellia specifications, soak them in clean water respectively, change the water every day, and drain the water twice during the water change process, wherein the raw pinellia is regularly sprayed with hydrogen peroxide silver ion composite solvent on the leaf surface of the raw pinellia during the cultivation stage; Add alum to the soaked raw pinellia, stir evenly, pickle for a preset time, add clean water to soak for a second time, and dry after soaking, wherein 5 kg of alum is used for every 100 kg of raw pinellia; Pour the dried sample into a container, stir evenly with sapodilla, dried ginger, mint, tangerine peel, liquorice, alum, saltpeter, add boiling water to brew and stir, control the juice to exceed the medicine surface by 14cm-16cm, seal, soak in a cool place indoors, take out, wash, cut into thick slices, dry, and obtain French pinellia, wherein, for every 100kg of raw pinellia, use 3kg of sapodilla, dried ginger, mint, tangerine peel, liquorice, 12.5kg of alum, and 5kg of saltpeter; Grind pinellia tuber, adzuki bean and bitter almond into fine powder, mix evenly with flour, add decoction of fresh artemisia annua, fresh polygonum hydropiper and fresh Xanthium sibiricum, and drop litsea cubeba oil, heat and stir evenly, ferment, shape and dry to obtain pinellia tuber. During the above drying processes, ultraviolet light from a high-pressure mercury lamp is used for irradiation.

2. The method for preparing the Banxiaqu according to claim 1, characterized in that: The method comprises the steps of grinding pinellia tuber, adzuki bean and bitter almond into fine powder, mixing the powder with flour, adding decoction of fresh artemisia annua, fresh polygonum hydropiper and fresh xanthium sibiricum, dripping litsea cubeba oil, heating and stirring, fermenting, shaping and drying to obtain pinellia tuber koji: Grind pinellia tuber, adzuki bean and bitter almond into fine powder, mix evenly with flour, add decoction of fresh Artemisia annua, fresh Polygonum hydropiper and fresh Xanthium sibiricum; Divide the Litsea cubeba oil into three equal parts and add them dropwise into the above mixture three times; The process is heated and stirred evenly, fermented, formed, and dried to obtain the pinellia tuber.

3. The method for preparing the Banxiaqu according to claim 2, characterized in that: During the irradiation with ultraviolet light from a high-pressure mercury lamp, the temperature of the surface of the medicinal material irradiated by the ultraviolet light from the high-pressure mercury lamp is controlled to be above 268°C.

4. The method for preparing the Banxiaqu according to claim 3, characterized in that: The drying process is as follows: firstly, the medicinal materials to be dried are placed in a drying box, in which a high-pressure mercury lamp ultraviolet device is also installed. While controlling the operation of the drying box, the high-pressure mercury lamp ultraviolet device is controlled to operate intermittently, wherein the operating time of the high-pressure mercury lamp ultraviolet device accounts for 5% to 10% of the total drying time.

5. The method for preparing Pinellia tuber according to claim 4, characterized in that: The drying temperature of the drying oven is set to 40°C ~ 50°C, and the drying time of the drying oven is set to 4h ~ 6h.

6. The method for preparing Pinellia tuber according to claim 5, characterized in that: According to the drying time of the drying box, the total number of operations of the high-pressure mercury lamp ultraviolet device is determined, wherein the total number of operations of the high-pressure mercury lamp ultraviolet device is 1 to 2 times for every 1 hour of drying, and the time interval between two operations of the high-pressure mercury lamp ultraviolet device is gradually extended.

7. The method for preparing Pinellia tuber according to claim 2, characterized in that: In the step of dividing the litsea cubeba oil into three equal parts and dropping them into the above mixture three times, the mass ratio of the litsea cubeba oil added to the fine powder of pinellia tuber, adzuki bean and bitter almond is 1:

100.

8. The method for preparing Pinellia tuber according to claim 2, characterized in that: In the step of heating and stirring uniformly, the heating temperature is 40° C. to 50° C., and the stirring rate is 20 r / min to 80 r / min.

9. The method for preparing Pinellia tuber according to claim 2, characterized in that: In the fermentation step, the fermentation temperature is 30° C. to 35° C., and the fermentation days are 4 to 5 days.

10. A Banxiaqu, characterized in that: It is prepared by the method for preparing the pinellia tuber according to any one of claims 1 to 9.

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