Antibacterial dressing containing plant essential oil

Abstract

The present invention relates to the technical field of dressings, and discloses an antibacterial dressing containing plant essential oils, wherein the antibacterial dressing containing plant essential oils comprises the following raw materials: atractylodes macrocephala essential oil microcapsules, modified polyglutamic acid, ammonium persulfate, and deionized water; wherein the atractylodes macrocephala essential oil microcapsules are prepared by using guar gum and gelatin as wall materials and atractylodes macrocephala essential oil as core material to form inclusion compounds; and modified polyglutamic acid is prepared by reacting perillyl alcohol with polyglutamic acid. The present invention prepares atractylodes macrocephala essential oil microcapsules and modified polyglutamic acid to participate in the preparation process of the dressing, so that the prepared dressing has excellent antibacterial and anti-inflammatory effects, can effectively promote wound healing, reduce pigmentation and the possibility of scarring, and can play an effective role for a long time without frequent replacement, can effectively avoid secondary injuries, and relieve pain.

Description

Technical Field

[0001] The invention relates to the technical field of dressings, and in particular to an antibacterial dressing containing plant essential oil. Background Art

[0002] Dressing is a common material used in wound management. It is used for wound protection and treatment. To a certain extent, it can isolate external pollution, maintain a moist environment for the wound, and promote wound healing. However, in actual use, the effective ingredients of ordinary dressings have a short effect time and need to be replaced frequently, which not only increases the complexity of wound care, but may also cause secondary damage. Frequent replacement of dressings may also increase the risk of wound infection, interfere with the natural healing process, prolong the healing time, and bring additional pain to patients. At the same time, ordinary dressings have limited antibacterial and healing effects, and cannot effectively resist the invasion of bacteria and other microorganisms, increasing the risk of wound infection. Even after the wound heals, pigmentation and scar hyperplasia are prone to occur, affecting the overall aesthetics of the skin.

[0003] Therefore, the patent with the announcement number CN115300660B discloses an antibacterial dressing and a preparation method, by stacking an adhesive layer and an antibacterial composition layer together, and providing the adhesive layer with through holes distributed in an array, while ensuring the viscosity of the antibacterial dressing, the air permeability is increased, the comfort of use is improved, and the secondary injury of the user is reduced. At the same time, the antibacterial components of the antibacterial dressing can be slowly released through the through holes on the adhesive layer, which can make the antibacterial effect more durable. Although the patent is based on reality, it increases the service life of the dressing and solves the problem of frequent dressing changes. However, the antibacterial components in the patent are chemical substances such as chlorhexidine gluconate, chlorhexidine acetate, triclosan, benzalkonium chloride, povidone iodine, polyhexamethylene biguanide hydrochloride and silver-containing compounds, which may be irritating in actual use and are not conducive to wound healing. Summary of the invention

[0004] The purpose of the present invention is to provide an antibacterial dressing containing plant essential oils, which solves the following technical problems: (1) the antibacterial and anti-inflammatory effects of ordinary dressings are average, and they need to be replaced frequently during use; (2) the healing and repair effects of ordinary dressings are average, and the wound is prone to pigmentation and even scarring after healing;

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] An antibacterial dressing containing plant essential oil comprises the following raw materials in parts by weight: 20-30 parts of atractylodes macrocephala essential oil microcapsules, 30-50 parts of modified polyglutamic acid, 2-5 parts of ammonium persulfate, and 100-150 parts of deionized water; the atractylodes macrocephala essential oil microcapsules are prepared by using guar gum and gelatin as wall materials and atractylodes macrocephala essential oil as a core material to form an inclusion compound; the modified polyglutamic acid is prepared by reacting perillyl alcohol with polyglutamic acid.

[0007] Furthermore, the preparation of the Atractylodes macrocephala essential oil microcapsules comprises the following steps:

[0008] S1: grind the raw Atractylodes macrocephala slices through a 20-30 mesh sieve, place them in an extraction tank, set the CO2 flow rate, extract for 2-3 hours under the conditions of extraction pressure of 25-35 MPa and extraction temperature of 40-45°C, collect the product after the extraction, and obtain the core material Atractylodes macrocephala essential oil;

[0009] S2: placing guar gum in deionized water, stirring and mixing thoroughly, adding glycidyl methacrylate and a catalyst, heating to 80-90° C. and stirring for 5-6 hours, and collecting the product after reduced pressure distillation to obtain modified guar gum;

[0010] S3: placing modified guar gum and gelatin in deionized water, heating to 50-55° C., and stirring and mixing thoroughly to obtain a wall material solution;

[0011] S4: Place the Atractylodes macrocephala essential oil in the wall material solution, use a high-speed dispersing homogenizer to emulsify for 3-5 minutes, add glacial acetic acid solution to adjust the pH to 4-5, heat to 40-45°C and stir at a constant temperature for 8-10 hours, cool to 10-12°C, collect the product, and obtain Atractylodes macrocephala essential oil microcapsules.

[0012] In this scheme, the supercritical extraction method is used to extract the Atractylodes macrocephala essential oil from raw Atractylodes macrocephala slices, and the Atractylodes macrocephala essential oil is used as the core material. The epoxy group in the structure of glycidyl methacrylate and the hydroxyl group in the structure of guar gum undergo a ring-opening reaction to obtain modified guar gum with an olefin group. The modified guar gum and gelatin are used as wall materials, the Atractylodes macrocephala essential oil is used as the core material, the pH value is adjusted, and the Atractylodes macrocephala essential oil microcapsules are prepared by a composite coacervation method. The surface of the Atractylodes macrocephala essential oil microcapsule has active double bonds, which can participate in the preparation process of the dressing and are not easy to precipitate when used. The modified guar gum and gelatin are used as shell materials, which have good biocompatibility and will not produce rejection reactions with wounds. Guar gum has good wettability and lubricity, and can form a moist protective film on the wound, effectively reducing the evaporation of moisture from the wound, reducing the friction between the dressing and the wound, and effectively reducing pain. Gelatin has the function of activating macrophages and stopping bleeding, and can effectively promote hemostasis and healing of wounds. Using gelatin and guar gum as a shell layer can not only protect the core material, but also create an excellent healing environment for the wound, effectively reducing the pain of the wound. The Atractylodes macrocephala essential oil microcapsule has a core material of Atractylodes macrocephala essential oil extracted from raw Atractylodes macrocephala, which is rich in compounds such as atractylodes ketone, germacrene and atractylodes lactone, and has significant The anti-inflammatory effect can effectively prevent the occurrence of inflammatory reactions, reduce the risk of wound infection, and effectively promote wound healing. It also has an antioxidant effect, can reduce the damage to the skin caused by oxidative stress, and has a good protective effect on the skin. In addition, Atractylodes macrocephala essential oil can promote the regeneration of skin cells, effectively repair the skin, accelerate the wound healing process, and reduce the risk of scarring. In this scheme, Atractylodes macrocephala essential oil is used as the core material to prepare microcapsules, and the Atractylodes macrocephala essential oil is protected and sustained-released by a shell layer made of guar gum and gelatin. It can not only protect the Atractylodes macrocephala essential oil from the influence of environmental factors such as light, heat, and oxygen, and extend the stability and service life of the Atractylodes macrocephala essential oil, but also control the slow release of the Atractylodes macrocephala essential oil, so that it can exert a long-term anti-inflammatory and repair effect, so that the prepared dressing will not fail in the short term, reduce the frequency of dressing changes at the wound, and reduce the risk of secondary injury.

[0013] Furthermore, in step S1, the CO2 flow rate is 25-30 L / h.

[0014] Furthermore, in step S2, the catalyst is any one of tetrabutylammonium bromide, tetrabutylammonium chloride, and tetrabutylammonium hydroxide.

[0015] Furthermore, in step S4, the mass fraction of the glacial acetic acid solution is 10-12%.

[0016] Furthermore, the preparation of the modified polyglutamic acid comprises the following steps:

[0017] Put polyglutamic acid and perillyl alcohol in dimethyl sulfoxide, stir and mix them thoroughly, then add p-toluenesulfonic acid, heat and react, and collect the product after reduced pressure distillation to obtain modified polyglutamic acid.

[0018] In this scheme, under the action of p-toluenesulfonic acid, the carboxyl group in the polyglutamic acid structure and the hydroxyl group in the perillyl alcohol structure undergo esterification reaction to obtain a modified polyglutamic acid containing an olefinic group in the structure. This modified polyglutamic acid has good biocompatibility and moisturizing properties, and can provide a better healing environment for the wound. At the same time, polyglutamic acid can inhibit the activity of tyrosinase, effectively reduce the production of melanin, and reduce the possibility of melanin deposition after wound healing. Perillyl alcohol has excellent antibacterial and anti-inflammatory effects, can kill bacteria, and reduce infection. Chemically bonding perillyl alcohol to polyglutamic acid can effectively enhance the stability of perillyl alcohol and prevent it from precipitating when the dressing is used for a long time, resulting in a decrease in the antibacterial effect. At the same time, this modified polyglutamic acid structure also has double bonds, which can participate in the preparation process of the dressing and form a cross-linked network structure with the Atractylodes macrocephala essential oil microcapsules, which can not only enhance the stability of the dressing and enhance the binding ability with the skin, but also can encapsulate perillyl alcohol in the network structure, so that it can produce a sustained-release effect, further enhancing the antibacterial and anti-inflammatory effects and durability of the dressing.

[0019] Furthermore, the polyglutamic acid is γ-polyglutamic acid.

[0020] Furthermore, the temperature of the temperature-raising reaction is 90-105° C., and the time is 6-8 hours.

[0021] Furthermore, the preparation method of the dressing comprises the following preparation steps:

[0022] Step 1, fully stirring and mixing the Atractylodes macrocephala essential oil microcapsules, modified polyglutamic acid and deionized water to obtain a mixed solution;

[0023] Step 2: Add ammonium persulfate to the mixed solution, heat it to 60-65°C and stir it thoroughly for 5-6 hours, cool it to room temperature and collect the product to obtain a dressing.

[0024] Beneficial effects of the present invention:

[0025] The present invention prepares Atractylodes macrocephala essential oil microcapsules and modified polyglutamic acid and participates in the preparation process of the dressing, so that the prepared dressing has excellent antibacterial and anti-inflammatory effects, can effectively promote wound healing, reduce pigmentation and the possibility of scarring, can play an effective role for a long time, does not need to be frequently replaced, can effectively avoid secondary injuries, and relieve pain.

[0026] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0028] Figure 1 The present invention is a process flow chart of the Atractylodes macrocephala essential oil microcapsule. DETAILED DESCRIPTION

[0029] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The preparation methods of the Atractylodes macrocephala essential oil microcapsules and modified polyglutamic acid in the following examples and comparative examples of the present invention are as follows:

[0031] 1. Preparation of Atractylodes macrocephala essential oil microcapsules

[0032] S1: 150g of raw Atractylodes macrocephala slices were crushed and passed through a 20-mesh sieve, placed in an extraction tank, and extracted for 2h under the conditions of extraction pressure of 25MPa, CO2 flow rate of 25L / h, and extraction temperature of 40°C. After the extraction, the product was collected to obtain the core material Atractylodes macrocephala essential oil;

[0033] S2: 2.3 g of guar gum was placed in 50 ml of deionized water, and after being fully stirred and mixed, 2 g of glycidyl methacrylate and 0.03 g of tetrabutylammonium bromide were added, and the temperature was raised to 80° C. and stirred for 5 h. The product was collected after reduced pressure distillation to obtain modified guar gum;

[0034] S3: 2 g of modified guar gum and 2 g of gelatin are placed in 600 ml of deionized water, heated to 50° C., and stirred to obtain a wall material solution;

[0035] S4: Place 4.5 ml of Atractylodes macrocephala essential oil in the wall material solution, emulsify with a high-speed disperser homogenizer for 3 minutes, add 10% glacial acetic acid solution to adjust the pH to 4, heat to 40°C and stir at a constant temperature for 8 hours, cool to 10°C, collect the product, and obtain Atractylodes macrocephala essential oil microcapsules.

[0036] The Atractylodes macrocephala essential oil microcapsules were stored at -18°C for 12 h, and then freeze-dried at -80°C and 1 Pa for 12 h to obtain Atractylodes macrocephala essential oil microcapsule powder. The Atractylodes macrocephala essential oil microcapsule powder was placed in a funnel, filtered and washed with petroleum ether, and the mixture was repeated three times and then dried to constant weight. The washed Atractylodes macrocephala essential oil microcapsules were weighed, placed in a Soxhlet extraction apparatus, and extracted to obtain the Atractylodes macrocephala essential oil embedded in the microcapsules. The embedding rate of the Atractylodes macrocephala essential oil was calculated according to the following formula: embedding rate (%) = mass of the embedded Atractylodes macrocephala essential oil (g) × 100 / initial mass of the Atractylodes macrocephala essential oil (g); after calculation, the embedding rate of the Atractylodes macrocephala essential oil microcapsules was 81.2%.

[0037] Combination Figure 1 It can be seen that the preparation process of Atractylodes macrocephala essential oil microcapsules is to crush and sieve the raw Atractylodes macrocephala, then use supercritical extraction technology to obtain Atractylodes macrocephala essential oil, then react guar gum and glycidyl methacrylate under the action of tetrabutylammonium bromide to obtain modified guar gum, and then combine the modified guar gum with gelatin to obtain a wall material solution, add Atractylodes macrocephala essential oil to the wall material solution, and adjust the pH of the solution to acidity by homogenizing and emulsifying with a high-speed dispersing homogenizer and using glacial acetic acid. After heating and stirring, the product is cooled and collected to obtain Atractylodes macrocephala essential oil microcapsules. The Atractylodes macrocephala essential oil microcapsules prepared by this scheme can not only protect the Atractylodes macrocephala essential oil from the influence of environmental factors such as light, heat, and oxygen, and prolong the stability and service life of the Atractylodes macrocephala essential oil, but also control the slow release of the Atractylodes macrocephala essential oil, so that it can exert a long-term anti-inflammatory and repair effect, so that the prepared dressing will not fail in the short term, reduce the frequency of dressing replacement at the wound, and effectively reduce the risk of secondary injury.

[0038] 2. Preparation of modified polyglutamic acid

[0039] 3.5 g of γ-polyglutamic acid and 3 g of perillyl alcohol were placed in 80 ml of dimethyl sulfoxide, and after being fully stirred and mixed, 0.2 g of p-toluenesulfonic acid was added, and the temperature was raised to 90° C. to react for 6 h. The product was collected after reduced pressure distillation to obtain modified polyglutamic acid.

[0040] Example 1

[0041] Dressing preparation

[0042] Step 1, fully stirring and mixing 20 parts of Atractylodes macrocephala essential oil microcapsules, 30 parts of modified polyglutamic acid and 100 parts of deionized water to obtain a mixed solution;

[0043] Step 2: Add 2 parts of ammonium persulfate to the mixed solution, heat to 60°C and stir thoroughly for 5 hours, cool to room temperature and collect the product to obtain a dressing.

[0044] Example 2

[0045] Dressing preparation

[0046] Step 1, fully stirring and mixing 25 parts of Atractylodes macrocephala essential oil microcapsules, 40 parts of modified polyglutamic acid and 120 parts of deionized water to obtain a mixed solution;

[0047] Step 2: Add 3.5 parts of ammonium persulfate to the mixed solution, heat to 62°C and stir thoroughly for 5.5 hours, cool to room temperature and collect the product to obtain a dressing.

[0048] Example 3

[0049] Dressing preparation

[0050] Step 1, 30 parts of Atractylodes macrocephala essential oil microcapsules, 50 parts of modified polyglutamic acid and 150 parts of deionized water are fully stirred and mixed to obtain a mixed solution;

[0051] Step 2: Add 5 parts of ammonium persulfate to the mixed solution, heat to 65°C and stir thoroughly for 6 hours, cool to room temperature and collect the product to obtain a dressing.

[0052] Comparative Example 1

[0053] Dressing preparation

[0054] Step 1, 25 parts of Atractylodes macrocephala essential oil, 40 parts of modified polyglutamic acid and 120 parts of deionized water are fully stirred and mixed to obtain a mixed solution;

[0055] Step 2: Add 3.5 parts of ammonium persulfate to the mixed solution, heat to 62°C and stir thoroughly for 5.5 hours, cool to room temperature and collect the product to obtain a dressing.

[0056] Comparative Example 2

[0057] Dressing preparation

[0058] Step 1, fully stirring and mixing 25 parts of Atractylodes macrocephala essential oil microcapsules, 40 parts of γ-polyglutamic acid and 120 parts of deionized water to obtain a mixed solution;

[0059] Step 2: Add 3.5 parts of ammonium persulfate to the mixed solution, heat to 62°C and stir thoroughly for 5.5 hours, cool to room temperature and collect the product to obtain a dressing.

[0060] Performance Testing

[0061] (1) Antibacterial performance testing

[0062] The dressings prepared in Examples 1 to 3 and Comparative Examples 1 to 2 were used as samples, and 1 ml of activated Escherichia coli and Staphylococcus aureus were respectively dripped onto the sterilized sample surface, and cultured at 37°C for 24 h. 10 ml of sterile saline was added for dilution, and the diluted bacterial solution was spread on a solid culture medium, and cultured at 37°C for 48 h. The number of colonies on the culture medium was counted, and a blank experiment was performed at the same time. The antibacterial rate was calculated with reference to the following formula: Antibacterial rate (%) = (number of colonies in the blank experiment - number of colonies in the sample experiment) × 100% / number of colonies in the blank experiment; the test results are shown in Table 1:

[0063] Table 1

[0064]

[0065] As can be seen from Table 1, the dressings prepared in Examples 1 to 3 all have excellent antibacterial effects. In the dressing prepared in Comparative Example 1, Atractylodes macrocephala essential oil is directly added without coating, and the Atractylodes macrocephala essential oil is not protected, which affects the use effect of the Atractylodes macrocephala essential oil. Since modified polyglutamic acid is added, it has a broad-spectrum antibacterial effect, so the antibacterial effect is better. In the sample prepared in Comparative Example 2, γ-polyglutamic acid is directly added without modification, but Atractylodes macrocephala essential oil microcapsules are added therein, so it also has an antibacterial effect, but not as good as the examples.

[0066] (2) 60 mice weighing 24-26 g were taken, and the hair on the right side of the back spine of each mouse was trimmed. Under anesthesia, a circular wound with a diameter of 1 cm and a depth of 2 mm was formed using a scalpel. Ten mice were divided into a group, and the total number was six. The wounds were covered with the functional dressings prepared in Example 1 to Example 3 and Comparative Example 1 to Comparative Example 2. A control group was set up, and the control group was directly covered with traditional cotton yarn. The wound healing rates of the mouse wounds on the 1st day, the 5th day and the 10th day were observed. When changing the dressing, the wound area was measured, and the wound healing rate was calculated by the following formula: wound healing rate = (area before treatment - area after treatment) / area before treatment × 100%; at the same time, the scar repair at the wound was observed on the 50th day after the wound healed, and the scar was ≤1 mm higher than the normal skin surface as the standard for complete repair. After the scar was completely repaired and dropped for 3 days, the scar and the surrounding skin were observed for obvious color difference. The test results are shown in Table 2:

[0067] Table 2

[0068]

[0069] As can be seen from Table 2, the samples prepared in Examples 1 to 3 all have excellent ability to promote wound healing, and are not prone to color difference and scars. The sample prepared in Comparative Example 1 has a good initial wound healing effect, but the long-term healing effect is not as good as that in the embodiment. This is because the Atractylodes macrocephala essential oil is directly added to the sample without being protected, resulting in a slow scar repair effect. Since modified polyglutamic acid is added thereto, there is no obvious color difference between the scar and the surrounding skin. The sample prepared in Comparative Example 2 has a good wound healing effect, which is due to the addition of Atractylodes macrocephala essential oil microcapsules. In the control group, traditional cotton yarn is directly used as a dressing. It can be seen that the wound heals slowly, the scar is difficult to repair, and obvious color difference appears on the wound skin.

[0070] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0071] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the scope defined by the concept of the invention, they should all fall within the protection scope of the present invention.

Claims

1. An antibacterial dressing containing plant essential oil, characterized in that: The invention comprises the following raw materials in parts by weight: 20-30 parts of atractylodes macrocephala essential oil microcapsules, 30-50 parts of modified polyglutamic acid, 2-5 parts of ammonium persulfate, and 100-150 parts of deionized water; the atractylodes macrocephala essential oil microcapsules are prepared by using guar gum and gelatin as wall materials and atractylodes macrocephala essential oil as a core material to form an inclusion compound; the modified polyglutamic acid is prepared by reacting perillyl alcohol with polyglutamic acid; The preparation of the Atractylodes macrocephala essential oil microcapsules comprises the following steps: S1: grind the raw Atractylodes macrocephala slices through a 20-30 mesh sieve, place them in an extraction tank, set the CO2 flow rate, extract for 2-3 hours under the conditions of extraction pressure of 25-35 MPa and extraction temperature of 40-45°C, collect the product after the extraction, and obtain the core material Atractylodes macrocephala essential oil; S2: placing guar gum in deionized water, stirring and mixing thoroughly, adding glycidyl methacrylate and a catalyst, heating to 80-90° C. and stirring for 5-6 hours, and collecting the product after reduced pressure distillation to obtain modified guar gum; S3: placing modified guar gum and gelatin in deionized water, heating to 50-55° C., and stirring and mixing thoroughly to obtain a wall material solution; S4: placing the Atractylodes macrocephala essential oil in the wall material solution, emulsifying it with a high-speed dispersing homogenizer for 3-5 minutes, adding glacial acetic acid solution to adjust the pH to 4-5, heating it to 40-45° C. and stirring it at a constant temperature for 8-10 hours, cooling it to 10-12° C. and collecting the product to obtain Atractylodes macrocephala essential oil microcapsules; The preparation of the modified polyglutamic acid comprises the following steps: The polyglutamic acid and perillyl alcohol are placed in dimethyl sulfoxide, fully stirred and mixed, and then p-toluenesulfonic acid is added, the temperature is raised to react, and the product is collected after reduced pressure distillation to obtain modified polyglutamic acid; The preparation method of the dressing comprises the following preparation steps: Step 1, fully stirring and mixing the Atractylodes macrocephala essential oil microcapsules, modified polyglutamic acid and deionized water to obtain a mixed solution; Step 2: Add ammonium persulfate to the mixed solution, heat it to 60-65°C and stir it thoroughly for 5-6 hours, cool it to room temperature and collect the product to obtain a dressing.

2. The antibacterial dressing containing plant essential oil according to claim 1, characterized in that: In step S1, the CO2 flow rate is 25-30 L / h.

3. The antibacterial dressing containing plant essential oil according to claim 1, characterized in that: In step S2, the catalyst is any one of tetrabutylammonium bromide, tetrabutylammonium chloride, and tetrabutylammonium hydroxide.

4. The antibacterial dressing containing plant essential oil according to claim 1, characterized in that: In step S4, the mass fraction of the glacial acetic acid solution is 10-12%.

5. The antibacterial dressing containing plant essential oil according to claim 1, characterized in that: The polyglutamic acid is γ-polyglutamic acid.

6. The antibacterial dressing containing plant essential oil according to claim 1, characterized in that: The temperature of the temperature-raising reaction is 90-105° C. and the time is 6-8 hours.

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