Method for collecting natural insulin from bee saliva and application

Abstract

The invention discloses a method for collecting natural insulin from bee saliva and application, and relates to the technical field of fermented products, and the method comprises the following steps: firstly, selecting a healthy bee colony; healthy bees are stimulated in a non-harmful stimulation mode, so that the bees are promoted to secrete saliva; according to the method, saliva secreted by bees is collected, impurities in the saliva of the bees are separated through a physical method, the saliva is purified, and therefore the bee saliva containing natural insulin components is obtained; according to the method for collecting the natural insulin from the bee saliva and the application, the bee saliva is collected by taking the honey as a carrier, the natural insulin in the bee saliva has no side effect, the body burden of a patient for long-term medication is reduced, and compared with artificially synthesized insulin, the extraction process of the bee saliva is more economical and efficient, so that the production cost is directly reduced; the long-term stability and reliability of a natural insulin source are guaranteed, the dependence on limited resources is reduced, and ecological balance and environmental protection are promoted.

Description

Technical Field

[0001] The invention relates to fermentation product technology, and in particular to a method for collecting natural insulin from bee saliva and application thereof. Background Art

[0002] In the complex ecosystem of nature, bees are important pollinators and sugar converters, and the sugar metabolism process in their physiological mechanism is particularly noteworthy. Through their sophisticated digestive system and efficient enzyme catalysis system, bees can quickly convert the collected nectar (mainly sucrose, a disaccharide) into monosaccharides that are easy to absorb and utilize, such as glucose and fructose. This process not only provides a source of energy for the bees themselves, but also promotes the conversion of nectar into honey, storing rich nutrients for future use.

[0003] An in-depth analysis of the sugar conversion mechanism of bees shows that their saliva contains a variety of key enzymes, which play a decisive role in catalyzing the decomposition of disaccharides into monosaccharides. What is particularly important is that there are a series of fine regulatory mechanisms in the bees to ensure that these enzymes work at the appropriate time and space to avoid energy waste and metabolic disorders. In contrast, although the sugar metabolism system of humans and mammals is also quite complex, they often seem unable to cope with challenges such as high-sugar diets or insufficient insulin secretion. High blood sugar has become a global health problem. It not only affects the quality of life of patients, but may also cause a series of serious complications, such as cardiovascular disease, kidney disease, and nervous system damage.

[0004] Currently, high blood sugar is mainly controlled by injecting synthetic insulin clinically. Although this method relieves the patient's symptoms to a certain extent, its high cost, possible side effects and dependence caused by long-term use are still worrying. Summary of the invention

[0005] The purpose of the present invention is to provide a method for collecting natural insulin from bee saliva and its application, so as to solve the above-mentioned shortcomings in the prior art.

[0006] In order to achieve the above object, the present invention provides the following technical solution: a method for collecting natural insulin from bee saliva, comprising the following steps:

[0007] S1. First, select a healthy bee colony for use;

[0008] S2, stimulate healthy bees through non-harmful stimulation to induce the bees to secrete saliva;

[0009] S3. Collect the saliva secreted by the bees, separate the impurities in the bee saliva by physical methods, and purify the saliva to obtain the bee saliva containing natural insulin components.

[0010] Furthermore, the stimulation method described in S2 includes one or more combinations of sound wave stimulation, light stimulation or temperature regulation stimulation.

[0011] Furthermore, the physical method described in S3 includes filtration and centrifugation.

[0012] A method for collecting bee saliva using honey as a carrier comprises the following steps: mixing the natural insulin component obtained by the method for collecting natural insulin from bee saliva according to any one of claims 1 to 4 with honey, so that the honey becomes a natural carrier of the natural insulin component, and repeatedly brewing the mixture of natural insulin and honey to obtain high-concentration insulin honey.

[0013] Furthermore, the repeated brewing process includes multiple repeated heating, cooling and stirring steps.

[0014] A high-content natural insulin honey product, comprising the high-concentration insulin honey.

[0015] Application of a high-content natural insulin honey product in the preparation of a health product for regulating blood sugar levels.

[0016] The invention discloses an application of a high-content natural insulin honey product in preparing medicines for regulating blood sugar levels.

[0017] Compared with the prior art, the method and application of collecting natural insulin from bee saliva provided by the present invention has no side effects when used, reduces the physical burden of patients that may be caused by long-term medication, and improves the safety and comfort of treatment; compared with artificial synthetic insulin, the extraction process of bee saliva is more economical and efficient, which not only directly reduces the production cost, but also makes the price of the product more competitive in the market, thereby benefiting more patients who need this treatment;

[0018] By using honey as a carrier to gather bee saliva, a sustainable resource utilization system is constructed. As a common product in nature, honey production is cyclical and renewable, thus ensuring the long-term stability and reliability of the natural insulin source. This way of utilizing renewable resources helps reduce dependence on limited resources and promote ecological balance and environmental protection. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0020] Figure 1 A schematic diagram of a method flow chart provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0021] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0022] Embodiment 1:

[0023] See also Figure 1 , a method for collecting natural insulin from bee saliva, comprising the following steps:

[0024] S1. First, select a healthy bee colony for use;

[0025] The specific implementation method is to select honey bees from natural areas without pesticide pollution and with good ecological environment as candidate objects. Honey bees in these areas usually maintain their natural physiological state and healthy body;

[0026] The candidate bees are subjected to rigorous health checks, including observation of their behavioral patterns (such as whether they are active in flying and whether their collecting behavior is normal), appearance characteristics (such as body color, body shape, and whether they have abnormal secretions or lesions), and internal examinations (if conditions permit, the health of their internal organs can be tested by non-invasive means).

[0027] The saliva secreted by a healthy bee colony is rich in various nutrients, such as protein, amino acids, vitamins, minerals and various bioactive substances. These ingredients have various health functions for the human body, such as enhancing immunity, promoting cell regeneration, improving sleep quality, and anti-aging. Compared with unhealthy or sick bees, the content of these nutrients in the saliva of healthy bees is more stable and abundant.

[0028] A healthy bee colony means that they carry no or fewer pathogens in their bodies, so the saliva products they secrete are more secure in terms of safety, reducing the health risks for consumers caused by eating substandard products. In addition, the saliva products of healthy bees are also easier to meet hygiene standards during the production process, ensuring the purity and quality of the product.

[0029] The saliva secreted by a healthy bee colony is more stable in quality, and the bioactive substances rich in the saliva of healthy bees are also more easily absorbed and utilized by the human body, thereby improving the nutritional value and health effects of the product.

[0030] Given that different species of bees may have different physiological characteristics and saliva components, Chinese honey bees, European honey bees or Italian honey bees are preferred. These bee species have strong environmental adaptability and can survive and reproduce in different climatic and ecological conditions. This adaptability makes them widely distributed around the world and become an indispensable ecological element in nature. When faced with adverse factors such as natural disasters and diseases, these bees can show strong resistance to adversity, respond to challenges through group collaboration and physiological regulation, and maintain the stability and prosperity of the population.

[0031] S2, stimulate healthy bees through non-harmful stimulation to induce the bees to secrete saliva;

[0032] The stimulation methods include one or more combinations of sound wave stimulation, light stimulation or temperature regulation stimulation. They use gentle stimulation methods that do not cause stress or harm to bees, such as simulating the smell of natural food sources (such as the aroma of pollen and nectar), slight vibration stimulation (simulating the vibration feeling during flight) or using light of a specific wavelength to stimulate the saliva secretion mechanism of bees.

[0033] Bees are sensitive to sound waves of specific frequencies. By finely controlling the sound wave parameters, their physiological responses can be triggered more effectively. Using sound waves of specific frequencies and intensities to simulate the sounds of wind, water or flower vibrations in nature may trigger the bees' foraging response and stimulate saliva secretion.

[0034] Mimicking the soft light changes at sunrise and sunset, or using ultraviolet light and other wavelengths that bees are sensitive to, may be closer to their perceptual experience in their natural environment and promote the natural secretion of saliva.

[0035] Bees are extremely sensitive to changes in environmental temperature, and a suitable temperature helps their physiological functions function normally. By precisely controlling the temperature in the collection environment and simulating the ideal temperature range for bees to be active, saliva secretion can be indirectly promoted. In addition, slight temperature differences may also serve as a signal to stimulate the physiological response of bees.

[0036] Using modern biotechnology and chemical synthesis technology, we can accurately replicate the odor molecules of natural food sources such as pollen and nectar. By releasing these odor molecules in trace amounts, we can attract bees and induce them to foraging behavior, thereby promoting saliva secretion.

[0037] During the stimulation process, ensure that the bees are in suitable environmental conditions, including appropriate temperature, humidity and light, to reduce the impact of external factors on the physiological state of the bees.

[0038] Before stimulation, prepare a clean, sterile saliva collector to ensure that the collected saliva is not contaminated.

[0039] S3. Collect the saliva secreted by the bees, separate the impurities in the bee saliva by physical methods, and purify the saliva to obtain the bee saliva containing natural insulin components.

[0040] After stimulation, check and collect the saliva secreted by the bees regularly. Be careful during the collection process to avoid causing unnecessary injury or stress to the bees.

[0041] The collected saliva contains a lot of impurities and solid particles, which require preliminary processing and filtration:

[0042] Sterile filter filtration: First, use a sterile filter to coarsely filter the saliva to remove large impurities and solid particles. You can use a filter with a moderate pore size, such as medical-grade sterile gauze or a stainless steel filter.

[0043] Centrifugal separation: The filtered saliva is centrifuged and the centrifugal force of the centrifuge is used to separate the tiny particles and impurities suspended in the saliva, ensuring that impurities are removed while retaining the target components.

[0044] After the initial treatment, saliva still contains many non-target components, which need to be further separated and purified:

[0045] Physical methods: In addition to centrifugation, physical methods such as ultrafiltration and dialysis can be used to further remove small molecule impurities and salts in saliva. These methods are based on the principle of molecular size and charge differences and can effectively separate the target components from other components.

[0046] Advanced purification technology: In order to obtain high-purity target components, more advanced purification technology is required. Molecular sieve chromatography (gel filtration chromatography) uses a gel with a mesh structure as the stationary phase, and achieves separation through the different relative molecular masses of the substances contained in the mobile phase. The gel is composed of a cross-linked porous mesh structure with different pore sizes, which can separate different components according to molecular size; ion exchange chromatography uses the interaction between charged molecules and ion exchange resins for separation. This technology uses the reversible exchange between the fixed charge on the ion exchanger and the charge carried by the solute molecules to be separated. By adjusting the pH value of the eluent or changing the concentration of like ions, the binding force of the solute molecules on the ion exchanger changes, and then they are eluted one by one in the order of the strength of the binding force, thereby achieving the purpose of separation; affinity chromatography uses the specific binding between the target component and the specific ligand for separation to obtain the best purification effect.

[0047] The purified target components need to undergo rigorous quality testing and evaluation to ensure that they meet relevant standards and requirements.

[0048] Purity testing: The purified samples are tested for purity using analytical methods such as high performance liquid chromatography (HPLC) and mass spectrometry (MS). These technologies can accurately measure the content and proportion of each component in the sample to evaluate its purity level; and verify whether the purified target component has the expected insulin-like biological activity.

[0049] Embodiment 2:

[0050] This embodiment provides a technical solution based on the first embodiment: a method for collecting bee saliva using honey as a carrier, comprising the following steps: mixing the natural insulin component obtained by the method of collecting natural insulin from bee saliva with honey, so that the honey becomes a natural carrier of the natural insulin component, and repeatedly brewing the mixture of natural insulin and honey to obtain high-concentration insulin honey; the repeated brewing process includes repeated heating, cooling and stirring steps.

[0051] A specific implementation method is to heat the mixture at a suitable temperature. This process is intended to promote enzyme activity and physical property changes in honey, while helping to stabilize the distribution of insulin in honey. Pay attention to controlling the heating temperature to avoid excessively high temperatures that may cause insulin denaturation.

[0052] After heating, the mixture is quickly cooled to room temperature or a specific temperature to fix the state of insulin in honey and reduce the loss of nutrients;

[0053] During the heating and cooling process, stir at appropriate times to ensure that the mixture is always in a uniform state and promote the deep integration of insulin and honey; repeated steps can increase the concentration and stability of insulin in honey.

[0054] Embodiment three:

[0055] This embodiment provides a technical solution based on the first embodiment: a high-content natural insulin honey product, which has high-concentration insulin honey.

[0056] The specific implementation method is to mix the collected natural insulin components with the prepared honey in a certain proportion. During the mixing process, low-speed stirring can be used to avoid excessive foaming; the mixture is repeatedly brewed. The specific steps include: first, heating the mixture to a suitable temperature (40-50°C) and maintaining it for a period of time to promote the dissolution and stability of insulin in honey; then, quickly cooling the mixture to room temperature; then, stirring to ensure the uniform distribution of insulin in honey; this process is repeated multiple times (3-5 times) to enhance the stability and activity of insulin.

[0057] The high-content natural insulin honey products are subjected to comprehensive quality testing after brewing. The testing items include insulin content (determined by high-performance liquid chromatography and other methods), purity, stability (assessed by accelerated stability tests), taste, and compliance with relevant food safety standards.

[0058] Use food-grade, well-sealed glass bottles or PET plastic bottles as packaging materials to ensure that the product is not contaminated during storage and transportation; mark the product name, production date, shelf life, storage conditions, consumption methods and precautions on the packaging; store the packaged products in a cool, dry and ventilated place, avoiding direct sunlight and high temperature environment to extend the shelf life of the product.

[0059] The collected high-concentration natural insulin is mixed with honey in a predetermined proportion. In order to ensure the uniform distribution and stability of insulin in honey, advanced mixing technology can be used, such as ultrasound-assisted mixing or high-pressure homogenization technology. During the mixing process, an appropriate amount of stabilizers, such as sucrose fatty acid esters, glyceryl stearate, etc., are added to prevent insulin from degradation or precipitation during storage and transportation. These stabilizers should comply with food safety standards and not affect the overall taste and nutritional value of the product.

[0060] In order to enhance the taste, nutritional value or functionality of the product, appropriate amounts of auxiliary materials can be added. The following are some possible auxiliary materials and their functions:

[0061] Natural sweeteners: such as monk fruit extract and erythritol, which are used to adjust the sweetness of the product while maintaining low calorie properties;

[0062] Vitamins and minerals: such as vitamin C, vitamin E, zinc, selenium, etc., to enhance the nutritional value and antioxidant capacity of the product;

[0063] Plant extracts: such as wolfberry extract and green tea extract, which have health functions such as regulating blood sugar and enhancing immunity;

[0064] Natural flavors: such as lemon oil, peppermint oil, etc., used to enhance the aroma and taste of the product;

[0065] The amount of excipients added should be adjusted according to the specific formula and product requirements, and ensure compliance with relevant regulations and standards.

[0066] The mixture with added excipients is homogenized to ensure that all ingredients are evenly distributed in the honey to form a stable product system.

[0067] The final product is subjected to comprehensive quality testing, including insulin content, purity, stability, taste and microbiological indicators.

[0068] Use packaging materials that meet food safety standards, and mark the packaging with information such as product name, production date, shelf life, storage conditions, consumption methods and precautions.

[0069] The addition of excipients is not only a key link in process requirements, but also the cornerstone for ensuring product safety and effectiveness. Following the golden principle of "safety, effectiveness and appropriate amount" is the primary prerequisite for ensuring the quality of insulin preparations.

[0070] Safety is the primary consideration in the selection of excipients. All added excipients must undergo strict toxicological evaluation to ensure that they are non-toxic and harmless to the human body and will not cause allergic reactions or other adverse events. This requires manufacturers to select verified, safe and reliable raw materials in accordance with relevant domestic and international laws and regulations and guidelines when selecting excipients.

[0071] The effectiveness of excipients is directly related to the overall performance of insulin preparations. They need to be able to stabilize the molecular structure of insulin to prevent it from being degraded or denatured during production, storage and use, thereby ensuring that the biological activity of insulin is not affected. At the same time, excipients should also help improve the solubility, stability, permeability and other physical and chemical properties of the preparation, and enhance the patient's medication experience and therapeutic effect.

[0072] Adding the right amount of excipients is an important means to ensure product quality. Excessive or insufficient excipients may have adverse effects on insulin preparations, such as affecting the release rate of the drug, causing local irritation or systemic reactions, etc.

[0073] Application of a high-content natural insulin honey product in the preparation of a health product for regulating blood sugar levels.

[0074] The specific implementation method is to design a reasonable health product formula according to the specific needs of diabetic patients and people at risk of high blood sugar. In addition to high-content natural insulin honey, the formula also contains other ingredients that help regulate blood sugar, such as dietary fiber, vitamins, minerals, and plant extracts with blood sugar-lowering effects.

[0075] Each component is accurately weighed according to the formula ratio and added to the mixing equipment in sequence for uniform mixing. If necessary, the mixture is further processed, such as granulation, tableting or filling into oral liquid, so that it is easy for consumers to eat.

[0076] The prepared health products are subjected to comprehensive quality testing, including verification of insulin content, purity, stability, taste, microbial indicators and blood sugar regulating function; when verifying its blood sugar regulating function, animal experiments or human clinical trials can be used to evaluate the effects of health products in lowering blood sugar levels, improving insulin resistance, etc.

[0077] The invention discloses an application of a high-content natural insulin honey product in preparing medicines for regulating blood sugar levels.

[0078] According to the needs of the target population and the results of clinical trials, a reasonable drug formula is designed, and high-content natural insulin honey, stabilizers and solubilizers are mixed in a certain proportion; appropriate preparation processes (such as capsule filling, tablet pressing, etc.) are used to make the mixture into an easy-to-take drug form.

[0079] Finished products are subjected to strict quality testing, including content determination, microbial limit testing, stability testing, etc., to ensure product safety and effectiveness.

[0080] Conduct clinical trials on the prepared drugs for regulating blood sugar levels, observe and record the blood sugar changes and adverse reactions of the subjects, so as to evaluate their efficacy and safety. Based on the test results, continuously optimize the product formula and preparation process to improve product performance.

[0081] Embodiment 4:

[0082] This embodiment provides a technical solution based on the first embodiment: a comparative test:

[0083] First, a certain number of subjects were selected from diabetic patients who met specific inclusion criteria. These patients were required to have stable high blood sugar levels and not receive other hypoglycemic treatments except diet control. Subsequently, these patients were divided into two groups, namely the bee saliva treatment group and the synthetic insulin treatment group, using a random number table method. The key indicators of each group of patients, such as age, gender, disease course, and baseline blood sugar level, were balanced to eliminate potential bias factors.

[0084] During the trial, the two groups of patients received different treatment plans. Patients in the bee saliva treatment group took 5 grams of strictly processed and purified bee saliva orally every day, while the synthetic insulin treatment group adjusted the dosage according to individual blood sugar conditions, with an average daily injection of about 15 grams of synthetic insulin. The treatment cycle was set for 8 consecutive weeks, during which patients were required to maintain the stability of their daily diet and exercise, and receive regular follow-up to monitor the treatment effect.

[0085] The main observation indicators of the trial include the patient's blood sugar level (fasting and 2 hours after meal blood sugar), changes in glycosylated hemoglobin (HbA1 c), and any side effects during treatment. Standardized blood sugar monitoring equipment and testing methods are used to ensure the accuracy and consistency of the data; at the same time, subjective indicators such as patient's physical changes, quality of life and satisfaction are recorded through questionnaires and clinical examinations.

[0086] After 8 weeks of treatment, the comparative test results showed encouraging results. After taking 5 grams of bee saliva, the blood sugar levels and glycosylated hemoglobin of the patients in the bee saliva treatment group were significantly reduced, and the reduction was comparable to that of patients receiving 15 grams of synthetic insulin. The patients in the bee saliva treatment group did not experience any obvious side effects during the treatment, while some patients in the synthetic insulin treatment group reported adverse reactions such as hypoglycemia, pain at the injection site, or allergies. In addition, from a cost-effectiveness perspective, the preparation cost of bee saliva is much lower than that of synthetic insulin, providing a more economical and feasible treatment option for diabetic patients.

[0087] As a natural product, bee saliva has a unique glucose-lowering mechanism and good safety, providing valuable clues for the development of new glucose-lowering drugs.

[0088] The above description is only by way of illustration of certain exemplary embodiments of the present invention. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A method for collecting natural insulin from bee saliva, characterized in that: The steps include: S1. First, select a healthy bee colony for standby use; S2, stimulate healthy bees through non-harmful stimulation to induce the bees to secrete saliva; S3. Collect the saliva secreted by the bees, separate the impurities in the bee saliva by physical methods, and purify the saliva to obtain the bee saliva containing natural insulin components.

2. A method for collecting natural insulin from bee saliva according to claim 1, characterized in that: S2 The stimulation method includes one or more combinations of sound wave stimulation, light stimulation or temperature regulation stimulation.

3. A method for collecting natural insulin from bee saliva according to claim 1, characterized in that: S3 The physical method includes filtration and centrifugation.

4. A method for collecting natural insulin from bee saliva according to claim 1, characterized in that: The purification in S3 is molecular sieve chromatography, ion exchange chromatography or affinity chromatography.

5. A method for collecting bee saliva using honey as a carrier, characterized in that: The method comprises the following steps: mixing the bee saliva containing natural insulin components obtained by the method for collecting natural insulin from bee saliva according to any one of claims 1 to 4 with honey, so that the honey becomes a carrier of the natural insulin components, and repeatedly brewing the mixture of the bee saliva containing natural insulin components and honey to obtain high-concentration insulin honey.

6. A method for collecting bee saliva using honey as a carrier according to claim 5, characterized in that: The repeated brewing process includes repeated heating, cooling and stirring steps.

7. A high-content natural insulin honey product, characterized in that: The high-content natural insulin honey product has the high-concentration insulin honey according to any one of claims 5-6.

8. Use of the high-content natural insulin honey product as claimed in claim 7 in the preparation of health products for regulating blood sugar levels.

9. Use of the high-content natural insulin honey product as claimed in claim 7 in the preparation of medicines for regulating blood sugar levels.

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