Low-purine krill oil and preparation method thereof

Abstract

The invention relates to the technical field of nutritional health-care products, in particular to low-purine krill oil and a preparation method thereof.The preparation method comprises the following steps that S1, antarctic krill powder is ground or smashed to ensure the efficiency and uniformity of the extraction process, and krill oil is extracted through an ultrasonic-assisted extraction method; s2, degreasing the extracted krill oil by a low-temperature freezing and centrifugal separation method; s3, degrading acidic components in the krill oil by adopting an enzymolysis method; s4, reducing the purine content in the krill oil by using an ultrafiltration technology and an ion exchange technology; and S5, purifying the mixed krill oil by adopting a molecular distillation method to obtain a low-purine krill oil finished product. By optimizing the extraction and treatment process, the purine content in the krill oil is effectively reduced, the stability of the krill oil is improved, meanwhile, the integrity of nutritional ingredients is kept, and the requirements of the market for high-quality health-care products are met.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional health products, and in particular to low-purine krill oil and a preparation method thereof. Background Art

[0002] As a new type of nutritional health product, low-purine krill oil has attracted widespread attention because it is rich in Omega-3 fatty acids, phosphatidylcholine and other ingredients that are beneficial to the human body. However, krill oil products on the market currently generally have the problem of high purine content. After purine metabolism, uric acid will be produced in the human body, which can easily cause diseases such as gout. In addition, krill oil is prone to oxidation reactions during the extraction and processing process, resulting in a decrease in product quality. Therefore, how to effectively reduce the purine content in krill oil and improve its stability and antioxidant properties has become a technical problem that needs to be solved urgently.

[0003] Existing technical methods, such as conventional physical extraction and chemical degradation, have limited effects in reducing purine content. There are also problems with complex processing, high costs, and easy destruction of other nutrients in krill oil. In addition, the existing technologies are not ideal in improving the stability and antioxidant properties of krill oil, and it is difficult to meet market demand.

[0004] Therefore, there is an urgent need for a new technical solution that can effectively reduce the purine content and enhance its stability and antioxidant properties while ensuring the high quality of krill oil, so as to meet consumers' demand for high-quality health products. Summary of the invention

[0005] Based on the above purpose, the present invention provides a low-purine krill oil and a preparation method thereof.

[0006] A method for preparing low-purine krill oil comprises the following steps: S1: Pretreatment, grinding or crushing the Antarctic krill powder to ensure the efficiency and uniformity of the extraction process, and extracting krill oil by ultrasound-assisted extraction; S2: defatting, the extracted krill oil is defatted by low-temperature freezing and centrifugal separation; S3: acid reduction treatment, using enzymatic hydrolysis to degrade the acidic components in krill oil; S4: Purine removal, using ultrafiltration and ion exchange technology to reduce the purine content in krill oil; S5: Purification: The mixed krill oil is purified by molecular distillation to remove residual impurities and obtain a low-purine krill oil product.

[0007] Furthermore, the frequency of the ultrasonic assisted extraction method in S1 is 20-40 kHz, the extraction time is 30-60 minutes, and the ultrasonic power is 200-500 W to extract the oil components in the krill meal.

[0008] Furthermore, the low-temperature freezing temperature in S2 is -20°C to -10°C, and the freezing time is 1-2 hours to reduce the oxidation reaction of oil; the centrifugal separation speed is 3000-5000rpm, and the centrifugal time is 10-20 minutes.

[0009] Furthermore, in the enzymatic hydrolysis method adopted in S3, the amount of enzyme added is 0.1-0.5% of the weight of the krill oil, the enzymatic hydrolysis temperature is 30-40° C., and the enzymatic hydrolysis time is 1-2 hours.

[0010] Furthermore, the membrane pore size of the ultrafiltration technology in S4 is 0.01-0.1 micron, the operating pressure is 0.1-0.5 MPa, and the ultrafiltration time is 30-60 minutes to remove the purine components in the krill oil; the ion exchange resin used in the ion exchange technology is a strong acid cation exchange resin, the resin amount is 5-10% of the weight of the krill oil, and the exchange time is 1-2 hours to reduce the purine content.

[0011] Furthermore, the operating temperature of the molecular distillation method in S5 is 50-80° C., the operating pressure is 1-10 Pa, and the distillation time is 1-2 hours.

[0012] Furthermore, a low-purine krill oil is prepared according to a method for preparing low-purine krill oil.

[0013] Beneficial effects of the present invention: The present invention, by adopting ultrasonic assisted extraction method and multi-step processing technology, effectively reduces the purine content in krill oil, solves the problem that traditional methods are difficult to completely remove purine, and further removes purine through low-temperature freezing and centrifugal separation degreasing treatment, combined with enzymatic degradation of acidic components, and ultrafiltration and ion exchange technology, to ensure the purity and safety of krill oil. In addition, the application of molecular distillation method significantly improves the stability and purity of krill oil, making the product less prone to oxidation and quality degradation during storage. The low-purine krill oil prepared by the method of the present invention not only has a low purine content, but also has good stability and shelf life, can better meet the market demand for high-quality health products, and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 The present invention is a schematic diagram of the preparation method of low-purine krill oil. DETAILED DESCRIPTION

[0016] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments.

[0017] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the present invention should be understood by people with ordinary skills in the field to which the present invention belongs. The "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0018] Example 1: Figure 1 As shown, a method for preparing low-purine krill oil comprises the following steps: S1: Pretreatment, grinding or crushing the Antarctic krill powder to ensure the efficiency and uniformity of the extraction process, and extracting krill oil by ultrasound-assisted extraction; S2: defatting, the extracted krill oil is defatted by low-temperature freezing and centrifugal separation; S3: acid reduction treatment, using enzymatic hydrolysis to degrade the acidic components in krill oil; S4: Purine removal, using ultrafiltration and ion exchange technology to reduce the purine content in krill oil; S5: Purification: The mixed krill oil is purified by molecular distillation to remove residual impurities and obtain a low-purine krill oil product.

[0019] The frequency of the ultrasonic assisted extraction method in S1 was 30 kHz, the extraction time was 45 min, and the ultrasonic power was 350 W to extract the oil components in the krill meal.

[0020] The low-temperature freezing temperature in S2 is -15°C and the freezing time is 1.5 hours to reduce the oxidation reaction of oils and fats; the centrifugal separation speed is 4000rpm and the centrifugal time is 15 minutes. The centrifugal force can separate the oils and fats from other components to ensure the purity of the extract.

[0021] In the enzymatic hydrolysis method adopted by S3, the enzyme addition amount is 0.3% of the weight of krill oil, the enzymatic hydrolysis temperature is 35°C, the enzymatic hydrolysis time is 1.5 hours, and lipase or protease is used to degrade the acidic components to improve the quality of krill oil.

[0022] The membrane pore size of the ultrafiltration technology in S4 is 0.05 microns, the operating pressure is 0.3MPa, and the ultrafiltration time is 45 minutes to remove the purine components in krill oil; the ion exchange resin used in the ion exchange technology is a strong acid cation exchange resin, the resin amount is 7% of the weight of the krill oil, and the exchange time is 1.5 hours to reduce the purine content.

[0023] The operating temperature of the molecular distillation method in S5 is 65°C, the operating pressure is 5Pa, and the distillation time is 1.5 hours. The residual impurities can be removed by molecular distillation to improve the purity of krill oil.

[0024] Example 2: The preparation process is as follows: S1: Pretreatment, grinding or crushing the Antarctic krill powder to ensure the efficiency and uniformity of the extraction process, and extracting krill oil by ultrasonic assisted extraction, with a frequency of 20kHz, an extraction time of 30 minutes, and an ultrasonic power of 200W; S2: Degreasing treatment: firstly, the extracted krill oil is subjected to low-temperature freezing treatment at -20°C for 1 hour to reduce the oxidation reaction of the oil. Then, it is centrifuged at a speed of 3000rpm for 10 minutes; S3: acid reduction treatment, specifically using enzymatic hydrolysis to degrade the acidic components in the krill oil, the amount of enzyme added is 0.1% of the weight of the krill oil, the enzymatic hydrolysis temperature is 30°C, and the enzymatic hydrolysis time is 1 hour; S4: Purine removal, using ultrafiltration technology and ion exchange technology to reduce the purine content in krill oil. The membrane pore size of ultrafiltration technology is 0.01 micron, the operating pressure is 0.1MPa, the ultrafiltration time is 30 minutes, and the ion exchange technology uses strong acid cation exchange resin, the resin amount is 5% of the weight of krill oil, and the exchange time is 1 hour; S5: The mixed krill oil is purified by molecular distillation to remove residual impurities. The operating temperature is 50° C., the operating pressure is 1 Pa, and the distillation time is 1 hour.

[0025] Example 3: The preparation process is as follows: S1: Pretreatment, grinding or crushing the Antarctic krill powder to ensure the efficiency and uniformity of the extraction process, and extracting krill oil by ultrasonic assisted extraction, with a frequency of 40kHz, an extraction time of 60 minutes, and an ultrasonic power of 500W; S2: Degreasing treatment: firstly, the extracted krill oil is subjected to low-temperature freezing treatment at -10°C for 2 hours to reduce the oxidation reaction of the oil. Then, it is centrifuged at a speed of 5000rpm for 20 minutes; S3: acid reduction treatment, specifically using enzymatic hydrolysis to degrade the acidic components in the krill oil, the amount of enzyme added is 0.5% of the weight of the krill oil, the enzymatic hydrolysis temperature is 40°C, and the enzymatic hydrolysis time is 2 hours; S4: Purine removal, using ultrafiltration technology and ion exchange technology to reduce the purine content in krill oil. The membrane pore size of ultrafiltration technology is 0.1 micron, the operating pressure is 0.5MPa, the ultrafiltration time is 60 minutes, and the ion exchange technology uses strong acid cation exchange resin, the resin amount is 10% of the weight of krill oil, and the exchange time is 2 hours; S5: The mixed krill oil is purified by molecular distillation to remove residual impurities. The operating temperature is 80° C., the operating pressure is 10 Pa, and the distillation time is 2 hours.

[0026] Table 1 Comparison of finished product performance parameters: ; As can be seen from Table 1 above, Example 1 exhibits a lower purine content and a higher omega-3 fatty acid content, which is very beneficial for consumers who pursue health benefits. At the same time, the total antioxidant capacity and vitamin E content of Example 1 also show good antioxidant properties, which help to improve the shelf life and nutritional value of the product. In addition, its excellent sensory evaluation further proves its superiority in taste and flavor. In terms of storage stability, Example 1 can maintain product stability for a long time, which is of great significance for the commercial circulation of the product. Therefore, Example 1 not only performs well in technical indicators, but also shows great potential in market and consumer acceptance.

[0027] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for preparing low-purine krill oil, It is characterized in that The following steps are involved: S1: Pretreatment, grinding or crushing the Antarctic krill powder to ensure the efficiency and uniformity of the extraction process, and extracting krill oil by ultrasound-assisted extraction; S2: defatting, the extracted krill oil is defatted by low-temperature freezing and centrifugal separation; S3: acid reduction treatment, using enzymatic hydrolysis to degrade the acidic components in krill oil; S4: Purine removal, using ultrafiltration and ion exchange technology to reduce the purine content in krill oil; S5: Purification: The mixed krill oil is purified by molecular distillation to remove residual impurities and obtain a low-purine krill oil product.

2. A method for preparing low-purine krill oil according to claim 1, It is characterized in that The frequency of the ultrasonic assisted extraction method in S1 is 20-40 kHz, the extraction time is 30-60 minutes, and the ultrasonic power is 200-500 W to extract the oil components in the krill powder.

3. The method for preparing low-purine krill oil according to claim 1, It is characterized in that The low-temperature freezing temperature in S2 is -20°C to -10°C, and the freezing time is 1-2 hours to reduce the oxidation reaction of oil; the rotation speed of centrifugal separation is 3000-5000rpm, and the centrifugal time is 10-20 minutes.

4. The method for preparing low-purine krill oil according to claim 1, It is characterized in that In the enzymatic hydrolysis method adopted in S3, the amount of enzyme added is 0.1-0.5% of the weight of the krill oil, the enzymatic hydrolysis temperature is 30-40° C., and the enzymatic hydrolysis time is 1-2 hours.

5. The method for preparing low-purine krill oil according to claim 1, It is characterized in that The membrane pore size of the ultrafiltration technology in S4 is 0.01-0.1 micron, the operating pressure is 0.1-0.5 MPa, and the ultrafiltration time is 30-60 minutes to remove the purine components in the krill oil; the ion exchange resin used in the ion exchange technology is a strong acid cation exchange resin, the resin amount is 5-10% of the weight of the krill oil, and the exchange time is 1-2 hours to reduce the purine content.

6. The method for preparing low-purine krill oil according to claim 1, It is characterized in that The operating temperature of the molecular distillation method in S5 is 50-80° C., the operating pressure is 1-10 Pa, and the distillation time is 1-2 hours.

7. A low-purine krill oil prepared according to the method according to any one of claims 1 to 6.

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