Arthrobacter capable of efficiently preventing and controlling tomato bacterial wilt and promoting tomato growth and application of arthrobacter

Abstract

The invention discloses arthrobacter capable of efficiently preventing and controlling tomato bacterial wilt and promoting tomato growth and application of the arthrobacter, and belongs to the technical field of agricultural microorganisms. The bacterial strain C152 is identified as a potential new species of Arthrobacter, and is named as Arthrobacter sp.C152, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC), and the preservation number is GDMCC No: 65249. The invention also discloses a method for preparing the Arthrobacter strain. The arthrobacter disclosed by the invention can produce siderophores and IAA, inhibit the growth of ralstonia solanacearum, significantly reduce the attack degree of tomato bacterial wilt (the disease resistance efficiency is up to 67.86%), and significantly promote the growth of tomato plants (the growth promoting efficiency is up to 20.30%). Therefore, the arthrobacter sp. C152 disclosed by the invention has important research value and application potential in the aspects of improving the bacterial wilt resistance of the crops and promoting the growth of the crops.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural microorganisms, and in particular to an Arthrobacter strain capable of effectively preventing and controlling tomato bacterial wilt and promoting tomato growth, and an application thereof. Background Art

[0002] Tomato is one of the three major world traded vegetables, known as the "crown of vegetables", and occupies an important economic position in the global vegetable trade. my country's annual tomato production is close to 1 / 3 of the world's total tomato production, ranking first in the world. Crop diseases are one of the most important limiting factors in tomato production, among which tomato bacterial wilt is particularly serious. Tomato bacterial wilt is a devastating soil-borne bacterial disease caused by Ralstonia solanacearum and is known as "tomato cancer." Due to the complex typing of the pathogen and its ability to survive for a long time in adverse environments, it is extremely difficult to prevent and control in agricultural production. Green prevention and control of crop diseases and crop production through microorganisms is considered to be the microbial technology with the most development potential.

[0003] There are rich varieties of microorganisms with different functions, especially those in the soil. Disease resistance and growth promotion are the two most concerned microbial functions in crop production. Studies have found that some microbial groups can improve plant disease resistance by secreting antibacterial compounds or enhancing plant immune responses, and some microorganisms can produce siderophores, IAA, or dissolve / mineralize insoluble phosphorus in the soil to promote plant growth. A few microbial groups have both functions. Therefore, screening and utilizing such multifunctional microorganisms, especially new microbial resources with outstanding functions after application, is very important for improving the application effect of microorganisms and solving the serious homogeneity of microbial products on the market. Summary of the invention

[0004] The purpose of the present invention is to provide a new disease-resistant and growth-promoting species of the genus Arthrobacter (Arthrobacter sp.) C152, so as to provide a new microbial resource for improving the resistance of crops to bacterial wilt and promoting crop growth.

[0005] To achieve the above purpose, the new species C152 of the genus Arthrobacter provided by the present invention is isolated from tomato rhizosphere soil and named Arthrobacter sp. C152. The strain is deposited in Guangdong Microbiological Culture Collection Center (GDMCC), the address is 5th Floor, Building 59, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, the deposit number is GDMCC No: 65249, and the deposit date is October 14, 2024.

[0006] Identification characteristics of the Arthrobacter:

[0007] Strain C152 is a Gram-positive bacterium. On TSA medium, the single colony is round, light yellow, smooth, transparent, and has neat edges. The 16S rRNA gene sequence of strain C152 is 1526 bp long. Sequence comparison analysis showed that the strain is similar to Arthrobacter pokkalii P3B162. T The strain C152 has the highest similarity of 98.59%. Based on the results of comparative genome analysis, the average nucleotide identity between strain C152 and the model species of the genus Arthrobacter is 78.85-83.98%, and the digital DNA-DNA hybridization value is 21.60-27.50%. The results show that strain C152 represents a new species of the genus Arthrobacter.

[0008] The present invention found that strain C152 has the ability to produce siderophore.

[0009] The present invention found that strain C152 has the ability to produce IAA, which is 22.05 mg·L -1 .

[0010] The present invention finds that strain C152 can inhibit the growth of Ralstonia solanacearum, and the diameter of the inhibition zone is 21.67 mm.

[0011] The present invention finds that inoculating strain C152 into tomato root system can significantly reduce the incidence of tomato bacterial wilt, and the disease resistance efficiency is 67.86%.

[0012] The present invention finds that inoculating strain C152 into tomato root system can significantly promote the growth of tomato plants, with the growth-promoting effect of the aboveground part being 16.13%, the root system being 30.18%, and the whole plant being 20.30%.

[0013] The present invention also provides a microbial fertilizer and / or microbial preparation, characterized in that it contains the Arthrobacter C152, or its fermentation liquid, or the supernatant of its fermentation liquid as an active ingredient.

[0014] The present invention also provides the use of the above-mentioned Arthrobacter C152 or the above-mentioned microbial fertilizer and / or microbial preparation in producing siderophores.

[0015] The present invention also provides the use of the above-mentioned Arthrobacter C152 or the above-mentioned microbial fertilizer and / or microbial preparation in producing IAA.

[0016] The present invention also provides the use of the above-mentioned Arthrobacter C152, or its fermentation liquid, or the supernatant of its fermentation liquid, or the above-mentioned microbial fertilizer and / or microbial preparation in biological control of tomato bacterial wilt.

[0017] The present invention also provides the use of the above-mentioned Arthrobacter C152, or its fermentation liquid, or the supernatant of its fermentation liquid, or the above-mentioned microbial fertilizer and / or microbial preparation in promoting the growth of tomatoes.

[0018] Preferably, the crop is tomato.

[0019] The present invention has the following advantages over the prior art:

[0020] 1) Arthrobacter C152 is a new species different from other Arthrobacter bacteria.

[0021] 2) Arthrobacter C152 can produce not only siderophores but also IAA, with an IAA production capacity of 22.05 mg·L -1 .

[0022] 3) Arthrobacter C152 could inhibit the growth of Ralstonia solanacearum, and the diameter of the inhibition zone was 21.67 mm.

[0023] 4) Arthrobacter C152 can significantly reduce the incidence of tomato bacterial wilt, with an anti-disease efficiency of up to 67.86%.

[0024] 5) Arthrobacter C152 can significantly promote the growth of tomato plants, with the growth-promoting effects on the aboveground part, root system and the whole plant reaching 16.13%, 30.18% and 20.30%, respectively.

[0025] Therefore, the Arthrobacter C152 disclosed in the present invention can improve the resistance of crops to bacterial wilt and promote crop growth, and has broad application prospects in the preparation of crop microbial fertilizers and microbial preparations.

[0026] Arthrobacter sp.C152 is deposited in the Guangdong Microbial Culture Collection Center (GDMCC), located at 5th Floor, Building 59, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, the deposit number is GDMCC No: 65249, and the deposit date is October 14, 2024. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is the colony morphology of Arthrobacter C152 on TSA medium.

[0028] Figure 2 is the phylogenetic tree of the genome of Arthrobacter C152.

[0029] Figure 3 It is the ability of Arthrobacter C152 to produce siderophore (A) and inhibit the growth of bacterial wilt pathogen (B).

[0030] Figure 4 This is the growth status of tomatoes when sampling was performed after inoculation with Arthrobacter C152.

[0031] Figure 5 The effect of inoculation with Arthrobacter C152 on tomato bacterial wilt and plant growth. DETAILED DESCRIPTION

[0032] The following are specific implementation examples of the present invention. It should be noted that these embodiments are only examples and do not constitute any limitation to the scope of the present invention. Modifications and replacements made to the details and forms of the implementation scheme within the scope of the present invention fall within the protection scope of the present invention.

[0033] Unless otherwise defined, the technical terms used in the following examples have the same meanings as those generally understood by those skilled in the art to which the present invention belongs. The experimental reagents used in the following examples are all conventional biochemical reagents unless otherwise specified.

[0034] Example 1: Isolation of strain C152

[0035] The soil used to isolate strain C152 was tomato soil collected from Jintang Town, Maonan District, Maoming City, Guangdong Province. Soil bacteria were isolated according to the high-throughput isolation, culture and identification technology of bacteria. The specific operation is as follows: Take 1g of fresh soil, dilute it 5000 times and then 2000 times with sterile magnesium chloride solution and 10% TSB liquid culture medium in turn. Take 160μL of the dilution and add it to 48 96-well cell culture plates and culture it in the dark at 30℃ for 2 weeks. Evenly aspirate 10μL of bacterial solution from the wells of the culture plate to extract bacterial DNA by alkaline lysis method. Take 140μL of 80% (v / v) glycerol and add it to the remaining bacterial solution, which is preserved as a bacterial glycerol seed for subsequent bacterial activation. PCR amplification was performed using primers 799F / 1193R (V5-V7 region of 16S rRNA gene) and Green Taq Mix of Novozymes. The product was diluted 120 times and used as a template. PCR amplification was performed using 799F and 1193R primers with sequencing adapters (Illumina adapter) and barcodes (Barcode). The second round of PCR products was electrophoresed, and the approximately 550bp band was cut for purification and the product concentration was determined. Each 96-well plate was prepared according to 100ng, and the mixed library of 48 plates totaled 4800ng. High-throughput sequencing was performed at Beijing Novogene Technology Co., Ltd. The sequencing data was analyzed using the analysis process Culturome (https: / / github.com / YongxinLiu / Culturome) to determine the position of the cultured bacteria in the 96-well plate. 25μL of bacterial solution was drawn from the wells of the corresponding 96-well cell culture plate and activated on 1 / 2TSA medium. The bacteria were purified by multiple streaking method, and the purified bacterial strain was mixed with 25% glycerol (v / v) and stored in a -20℃ refrigerator to obtain strain C152.

[0036] The strain C152 was identified as a Gram-positive bacterium. The single colony on TSA medium was round, light yellow, smooth, surrounded by a transparent circle, and had neat edges ( Figure 1 ).

[0037] Example 2: 16S rRNA gene sequence analysis of strain C152

[0038] The genomic DNA of strain C152 was extracted using the HiPure Bacterial DNA Kit (Cat. No. D3146-02) of Guangzhou Meiji Biotechnology Co., Ltd., and the full length of the 16S rRNA gene was amplified using the bacterial universal primers 27F / 1492R. The PCR product was sent to Suzhou Jinweizhi Biotechnology Co., Ltd. for sequencing, and its sequence is shown in SEQ ID NO.1, with a length of 1526bp. The sequences obtained by sequencing were compared on the EzBioCloud (https: / / www.ezbiocloud.net / identify) website. The results showed that strain C152 and the model strain Arthrobacter pokkalii P3B162 T has the highest similarity of 98.59%; followed by Arthrobacter pascens DSM 20545 T , similarity 98.55%; Arthrobacterglobiformis NBRC 12137 T , with a similarity of 98.06%. The 16S rRNA gene sequence similarity with other model species of the genus Arthrobacter was lower than 98.00%. Based on the 16S rRNA gene sequence similarity results, strain C152 belongs to the genus Arthrobacter, but its taxonomic status cannot be accurately determined.

[0039] Example 3: Full gene sequence analysis of strain C152

[0040] Strain C152 was sent to Shanghai Meiji Biopharmaceutical Technology Co., Ltd. for genome sequencing using the Illumina Novaseq platform. SPAdes v3.15.3 software was used to assemble the bacterial genome sequence and remove contig sequences of low quality and less than 500bp in length. CheckM v1.1.3 software was used to perform quality analysis on the integrity and contamination of the bacterial genome. If the integrity of the genome is not less than 95% and the contamination is not more than 5%, the genome is considered to be of high quality. The analysis results showed that the genome integrity of strain C152 was 99.71% and the contamination was 0.34%, so the genome was a high-quality genome. The bacterial genome was analyzed using QUAST v5.0.2. The results showed that the total genome length of strain C152 was 4.69Mbp, the length of N50 was 119146bp, and the G+C content of genomic DNA was 66.95%.

[0041] The genome of the model species of the genus Arthrobacter was downloaded from the NCBI website (https: / / www.ncbi.nlm.nih.gov / datasets / genome / ?taxon=1663). In order to further clarify the differences between these bacteria, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between strain C152 and the genome sequence of the model species were calculated using ANICalculator (http: / / www.ezbiocloud.net / tools / ani) and Genome-to-Genome DistanceCalculator v3.0 (https: / / ggdc.dsmz.de / ggdc.php#). The analysis results showed that the ANI value between strain C152 and the model species was 78.85-83.98%, and the dDDH value was 21.60-27.50% (Table 1). When the ANI value of two bacterial genomes is 95-96%, it is equivalent to a DNA-DNA hybridization value of 70%, which is equivalent to a 16S rRNA gene similarity of 98.65%. Therefore, when the ANI values ​​of two bacterial genomes are greater than 96%, they are the same species; when the ANI values ​​of two bacterial genomes are less than 95%, they are different species. A whole-genome DNA-DNA hybridization value of 70% is the gold standard for the classification of prokaryotic species. In addition, UBCG v3.0 was used to construct a genomic phylogenetic tree of strain C152 and closely related reference model strains. The analysis results showed that strain C152 formed an independent phylogenetic branch with a bootstrap value of 100( Figure 2 ), indicating that strain C152 has an independent taxonomic status. Based on the above results, strain C152 is a potential new species of the genus Arthrobacter, named Arthrobacter sp. C152. The strain is deposited in Guangdong Microbiological Culture Collection Center (GDMCC), the address is 5th Floor, Building 59, No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, Postal Code: 510070, the deposit number is GDMCC No: 65249, and the deposit date is October 14, 2024.

[0042] Table 1 ANI and dDDH values ​​between Arthrobacter C152 and type species of Arthrobacter

[0043]

[0044] Example 4: Identification of the ability of Arthrobacter C152 to produce siderophore

[0045] Bacterial iron carrier identification culture medium: Chrome azurol S (CAS) 60.5 mg·L -1 , hexadecyltrimethylammonium bromide (HDTMA) 72.9 mg·L -1 , ferric chloride hexahydrate 2.645 mg·L -1 , sodium dihydrogen phosphate dihydrate 295.25 mg·L -1 , disodium hydrogen phosphate dodecahydrate 1213.5 mg·L -1 , ammonium chloride 125mg·L -1 , potassium dihydrogen phosphate 37.5mg·L -1 , sodium chloride 62.5mg·L -1 , pH 6.8 ± 0.1. The culture medium was purchased from Qingdao High-Tech Industrial Park Haibo Biotechnology Co., Ltd., with the catalog number HB9132.

[0046] Activate Arthrobacter C152 on TSA medium, pick a single colony and inoculate it into 50mL TSB liquid medium, and culture it at 30℃ and 200rpm for 2 days. Pipette 10μL of bacterial solution and add it to the bacterial siderophore production identification medium, then invert the plate and culture it at 30℃ for 5 days. Observe the orange-yellow circle on the plate to determine whether Arthrobacter C152 has the ability to produce siderophores.

[0047] The results showed that a huge orange-yellow ring ( Figure 3 A), indicating that Arthrobacter C152 has the ability to produce siderophores.

[0048] Example 5: Identification of the IAA production capacity of Arthrobacter C152

[0049] A single colony of activated Arthrobacter C152 was inoculated into a solution containing 200 mg·L -1 The cells were cultured in R2A liquid medium containing L-tryptophan at 30°C and 200 rpm for 2 days with 3 biological replicates. 1 mL of bacterial culture was taken and the supernatant was collected by centrifugation at 12000 rpm for 5 min. The IAA production was determined by the Salkowski colorimetric method. 100 μL of supernatant was mixed with an equal volume of Salkowski colorimetric solution (50 mL 35% HClO4 + 1 mL 0.5 mol·L -1 FeCl3) was mixed. After standing for 0.5 h in dark conditions, the absorbance at 530 nm was measured using an ELISA instrument. Sterile R2A liquid culture medium was used as a control. -1 The IAA standard solution was diluted in a gradient manner and the OD at the corresponding concentration was measured. 530 The absorbance value was used to draw a standard curve and calculate the amount of IAA produced by Arthrobacter C152.

[0050] The results showed that the IAA production capacity of Arthrobacter C152 was 22.05 mg·L -1 .

[0051] Example 6: Identification of the inhibitory effect of Arthrobacter C152 on bacterial wilt pathogens

[0052] The double-layer agar diffusion method was used to determine the inhibitory effect of Arthrobacter C152 on bacterial wilt pathogens. First, prepare the bottom TSA culture medium (agar powder content 1.6%) with a moderate thickness. Place Oxford cups on the bottom TSA culture medium, 3 on each plate. Then pour in an appropriate amount of NA culture medium and take out the Oxford cup after it solidifies. The bacterial wilt pathogen Ralstonia solanacearum QL-Rs1115 was inoculated into NB liquid culture medium for activation. After culturing for 24 hours, the bacteria in the fermentation broth were collected and their OD was adjusted. 600 =1.0, then dilute it with sterile water to a concentration of 0.8%, and apply the bacterial solution evenly on the NA medium with a cotton swab. Take 30 μL of OD 600 =1.0 was added into the wells, and after culturing at 28°C for 36 h, the diameter of the inhibition zone was measured.

[0053] The results showed that after the addition of Arthrobacter C152, the pathogen of bacterial wilt, Ralstonia solanacearum QL-Rs1115, formed a clear transparent circle around the well, with an average value of 21.67 mm ( Figure 3 B). This indicates that Arthrobacter C152 has a significant inhibitory effect on the growth of Ralstonia solanacearum QL-Rs1115, the pathogen of bacterial wilt.

[0054] Example 7: Evaluation of the ability of Arthrobacter C152 to resist tomato bacterial wilt and promote growth

[0055] The soil from which Arthrobacter C152 was isolated was used as the test soil. After the soil was air-dried, it was passed through a 2mm sieve and then sterilized by high-temperature and high-pressure steam (sterilized at 121°C for 1h). This experiment was a potted plant experiment, with a total of 4 treatments, namely CK (control group, i.e., only inoculated with water), P (only inoculated with the pathogen Ralstonia solanacearum QL-Rs1115), C152 (only inoculated with Arthrobacter C152) and C152-P (first inoculated with the pathogen and then inoculated with Arthrobacter C152). Each treatment had 6 biological replicates. After germination of the Xinzhongshu No. 4 tomato seeds, seeds with consistent germination were selected and sown in pots. After 14 days of growth, tomato seedlings with consistent growth were selected. The C152 and C152-P groups were inoculated with 5mL of the bacterial suspension of Arthrobacter C152 (resuspended in sterile water, OD 600=1.0), CK and P groups were inoculated with 5 mL of sterile water. 7 days later, P and C152-P groups were inoculated with 5 mL of Ralstoniasolanacearum QL-Rs1115 bacterial suspension (OD 600 =1.0), CK and C152 were inoculated with 5 mL of sterile water. After 10 days, the incidence of tomato bacterial wilt was counted. The incidence of tomato bacterial wilt is divided into 0 to 5 levels, representing no symptoms (0), 20% (1), 40% (2), 60% (3), 80% (4) and 100% (5) leaves with symptoms. The aboveground parts and roots of tomatoes were dried separately and their biomass was measured.

[0056] The results showed that the tomato seedlings in the CK and C152 groups did not show symptoms of bacterial wilt, while the tomato seedlings in the P and C152-P groups showed typical symptoms of bacterial wilt ( Figure 4 Compared with the P group, the incidence of tomato bacterial wilt in the C152-P group was significantly reduced (P<0.001, n=6), and the disease resistance efficiency of the inoculated Arthrobacter C152 was as high as 67.86% ( Figure 5 Compared with the CK group, the aboveground biomass, root biomass and plant biomass of the C152 group were significantly increased (P<0.001, n=6), and the growth promotion efficiency of inoculation with Arthrobacter C152 was as high as 16.13%, 30.18% and 20.30%, respectively ( Figure 5 ). In summary, Arthrobacter C152 is a multifunctional bacterium that is highly resistant to tomato bacterial wilt and can effectively promote tomato growth.

[0057] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

[0058] Full-length sequence of 16S rRNA gene of Arthrobacter C152 (SEQ ID NO.1)

[0059] TCAACGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATGC

[0060] AAGTCGAACGATGATCTCCAGCTTGCTGGGGGGATTAGTGGCGAACGGGTGAGTAACA

[0061] CGTGAGTAACCTGCCCTTGACTCTGGGATAAGCCTGGGAAACTGGGTCTAATACCGGA

[0062] TATGACTGACTATCGCATGGTGGTTGGTGGAAAGCTTTTGTGGTTTTGGATGGACTCGC

[0063] GGCCTATCAGCTTGTTGGTGGGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCC

[0064] TGAGAGGGTGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGC

[0065] AGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATGCAGCGACGCCGCGTGAGG

[0066] GATGACGGCCTTCGGGTTGTAAACCTCTTTCAGTAGGGAAGAAGCCGCAAGGTGACGG

[0067] TACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGC

[0068] GCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGTTTGTCGCGTCTG

[0069] CCGTGAAAGTCCGGGGCTCAACTCCGGATCTGCGGTGGGTACGGGCAGACTAGAGTGA

[0070] TGTAGGGGAGACTGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAAC

[0071] ACCGATGGCGAAGGCAGGTCTCTGGGCATTAACTGACGCTGAGGAGCGAAAGCATGG

[0072] GGAGCGAACAGGATTAGATACCCTGGTAGTCCATGCCGTAAACGTTGGGCACTAGGTG

[0073] TGGGGGACATTCCACGTTTTCCGCGCCGTAGCTAACGCATTAAGTGCCCCGCCTGGGG

[0074] AGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGCGG

[0075] AGCATGCGGATTAATTCGATGCAACGCGAAGAACCTTACCAAGGCTTGACATGGACCG

[0076] GACCGCCGCAGAAATGTGGTTTCCCCTTTGGGGCCGGTTTACAGGTGGTGCATGGTTGT

[0077] CGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGTTCT

[0078] ATGTTGCCAGCGGTTCGGCCGGGGACTCATAGGAGACTGCCGGGGTCAACTCGGAGGA

[0079] AGGTGGGGACGACGTCAAATCATCATGCCCCTTATGTCTTGGGCTTCACGCATGCTACA

[0080] ATGGCCGGTACAAAGGGTTGCGATACTGTGAGGTGGAGCTAATCCCAAAAAGCCGGTC

[0081] TCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTCGCTAGTAATCGC

[0082] AGATCAGCAACGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGT

[0083] CACGAAAGTTGGTAACACCCGAAGCCGGTGGCCTAACCCCTTGTGGGAGGGAGCTGTC

[0084] GAAGGTGGGACTGGCGATTGGGACTAAGTCGTAACAAGGTAGCCGTACCGGAAGGTG

[0085] CGGCTGGATCACCTCCTTT。

Claims

1. Arthrobacter sp. C152, deposited with GDMCC No: 65249.

2. A microbial fertilizer and / or microbial preparation, characterized in that: Contains the Arthrobacter C152 according to claim 1, or a fermentation liquid thereof, or a supernatant of a fermentation liquid thereof as an active ingredient.

3. Use of the Arthrobacter C152 according to claim 1 or the microbial fertilizer and / or microbial preparation according to claim 2 in producing siderophores.

4. Use of the Arthrobacter C152 described in claim 1 or the microbial fertilizer and / or microbial preparation described in claim 2 in producing IAA.

5. Use of the Arthrobacter C152 according to claim 1, or its fermentation liquid, or the supernatant of its fermentation liquid, or the microbial fertilizer and / or microbial preparation according to claim 2 in biological control of crop bacterial wilt.

6. Use of the Arthrobacter C152 according to claim 1, or its fermentation liquid, or the supernatant of its fermentation liquid, or the microbial fertilizer and / or microbial preparation according to claim 2 in promoting crop growth.

7. The use according to claim 5 or 6, characterized in that: The crop is tomato.

Images