A method and device for EMS directed mutagenesis and screening of alfalfa

Abstract

The invention discloses a method and device for EMS directional mutagenesis and screening of alfalfa, the method comprising planting alfalfa seeds soaked in a mutagen in a plant pellet; placing the plant pellets in batches on each mutagenesis culture tray; supplying a nutrient solution and a sodium chloride solution to each mutagenesis culture tray through a supply pipe system, and controlling the illumination of each mutagenesis culture tray; transferring the germinated alfalfa seeds to a test field for cultivation; and collecting alfalfa seeds with excellent mutations after the alfalfa matures. The device comprises a directional mutagenesis mechanism and an air suction feeding mechanism, the directional mutagenesis mechanism comprises a plurality of mutagenesis culture trays, the supply pipe system and the discharge pipe system are respectively connected to each mutagenesis culture tray, a transparent opening and closing cover is provided on the base, and the transparent opening and closing cover is covered with LED lighting lamps. The invention efficiently directional mutagenesis and cultivates alfalfa, so that it has extremely high salt tolerance, and ensures the survival rate and income of the salt-tolerant alfalfa obtained on saline-alkali land. The invention is applicable to the technical field of alfalfa cultivation and mutagenesis.

Description

Technical Field

[0001] The invention belongs to the technical field of alfalfa cultivation and mutagenesis, and specifically relates to a method and a device for EMS directed mutagenesis and screening of alfalfa. Background Art

[0002] At present, due to the low utilization rate of saline-alkali land, in the case of limited arable land resources, it is a waste of resources to be unable to effectively utilize saline-alkali land, and it is urgent to efficiently utilize saline-alkali land. Economic crops adapted to saline-alkali land need to have a certain salt tolerance, but this type of economic crops is relatively scarce. Even if one or more economic crops are found to be planted on saline-alkali land, they can be used to obtain certain economic benefits and improve the environment. However, on the one hand, the survival rate and income of economic crops are low, and on the other hand, the improvement of the soil environment is also minimal, which does not have an improvement effect on the productivity of subsequent saline-alkali land. Therefore, there is an urgent need for a mutagenesis and screening method to carry out directed mutagenesis and cultivation of some economic crops with strong vitality and high income, so that these economic crops have extremely high salt tolerance, in order to improve the efficiency of mutagenesis, and efficiently select seeds of economic crops with extremely high salt tolerance, in preparation for subsequent sowing on saline-alkali land. Summary of the invention

[0003] The invention provides a method and device for EMS directed mutagenesis and screening of alfalfa, which are used for efficiently directed mutagenesis and cultivation of alfalfa to make it have extremely high salt tolerance and ensure the survival rate and yield of the obtained salt-tolerant alfalfa on saline-alkali land.

[0004] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0005] A method for EMS directed mutagenesis and screening of alfalfa comprises the following steps:

[0006] Step 1. Make planting soil into spherical planting balls in batches, plant alfalfa seeds soaked in a mutagen ethyl methanesulfonate in the planting balls, and the number of alfalfa seeds in each planting ball is one;

[0007] Step 2. Use an air suction loading mechanism to place the plant pellets in batches on each mutagenesis culture tray of the directional mutagenesis mechanism;

[0008] Step 3. supplying the nutrient solution and the sodium chloride solution to each mutagenesis culture plate through the supply pipe system of the directional mutagenesis mechanism, and controlling the illumination of each mutagenesis culture plate at the same time;

[0009] Step 4. Transfer the germinated alfalfa seeds to the experimental field for cultivation;

[0010] Step 5. After the alfalfa matures, collect the alfalfa seeds with excellent mutations.

[0011] Furthermore, the alfalfa seeds collected in step 5 are soaked in a mutagen ethyl methanesulfonate, and then steps 1 to 5 are repeated to obtain salt-tolerant alfalfa seeds.

[0012] The present invention also discloses a device for the above-mentioned EMS directed mutagenesis and alfalfa screening method, comprising a directed mutagenesis mechanism arranged on a test bench or a laboratory floor, the directed mutagenesis mechanism comprising a plurality of mutagenesis culture trays installed on a base, the mutagenesis culture trays are arranged at intervals in a vertical direction, a supply pipe system and an exhaust pipe system are respectively connected to each mutagenesis culture tray, a transparent opening and closing cover for covering the mutagenesis culture trays is arranged on the base, and LED lighting lamps are arranged on the transparent opening and closing cover; an air suction feeding mechanism is arranged on one side of the directed mutagenesis mechanism and is located at the opening and closing mouth of the transparent opening and closing cover.

[0013] Furthermore, the mutagenesis culture tray includes a tray body with a nutrient cavity, and vegetation buckets are evenly constructed at the upper end of the tray body, and the lower end of each vegetation bucket extends to the lower part of the nutrient cavity. A supply joint and a discharge joint are respectively constructed on the lower end wall of the tray body, and the supply joint and the discharge joint are both connected to the nutrient cavity. The tray body is connected to two connecting rods, and the lower ends of the two connecting rods are fixed to the base.

[0014] Furthermore, the vegetation bucket includes a vegetation portion, a transition portion and a capillary portion which are sequentially connected in a vertical downward direction. The vegetation portion is a hemispherical shell-shaped structure, a vegetation groove is formed in the vegetation portion, the vegetation balls are placed in the vegetation groove, a filter layer is arranged in the transition portion, a capillary channel connected to the transition portion is constructed in the capillary portion, and the capillary channel is connected to the nutrient chamber.

[0015] Furthermore, a plurality of conducting holes are provided on the peripheral wall of the vegetation part, and these conducting holes are all connected to the nutrient chamber. An inclined liquid suction port is formed at the lower end of the capillary part, and a plurality of air guide holes are provided at intervals on the upper end wall of the disk body, and these air guide holes are all connected to the nutrient chamber.

[0016] Furthermore, the supply pipe system includes a supply hose constructed with multiple first joint pipes, a first control valve is installed on each of the first joint pipes, each first joint pipe is connected to a corresponding supply joint, a liquid supply pipe is installed at the inlet end of the supply hose, an air supply pipe is installed on the liquid supply pipe, and a liquid supply control valve and an air supply control valve are installed on the liquid supply pipe and the air supply pipe, respectively.

[0017] Furthermore, the discharge pipe system includes a discharge hose constructed with multiple second joint pipes, a second control valve is installed on each of the second joint pipes, each second joint pipe is connected to a corresponding discharge joint, a discharge channel is opened in the base, and a first connecting joint and a second connecting joint are respectively constructed on the base and located at both ends of the discharge channel, the first connecting joint is connected to the lower end of the discharge hose, and a discharge control valve is installed on the second connecting joint.

[0018] Furthermore, the air suction feeding mechanism includes an air suction cup installed at one end of the lifting and adjusting unit, the air suction cup includes a suction cup body having an air suction chamber, the upper end of the suction cup body is detachably connected with an upper end cover, a plurality of hemispherical adsorption grooves are constructed at the lower end of the suction cup body, each of the adsorption grooves is provided with an air suction hole connected to the air suction chamber, a connecting column connected to the transfer arm is constructed on the upper end cover, a first channel and a second channel connected to each other are respectively opened on the connecting column and the transfer arm, the first channel is connected to the air suction chamber, and an air suction joint connected to the second channel is constructed on the transfer arm.

[0019] Furthermore, the lifting adjustment unit includes a driving cylinder connected between the transfer arm and the connecting seat, a linear slide is installed on the connecting seat, and the linear slide is assembled on the vertical guide rail.

[0020] Due to the adoption of the above structure, the technical progress achieved by the present invention compared with the prior art is that: since the alfalfa seeds soaked in the mutagen ethyl methanesulfonate have extremely high multidirectional mutation ability, the alfalfa seeds can be subjected to directed mutagenesis; specifically, the soaked alfalfa seeds are placed in batches and layers in a plurality of mutagenesis culture trays of a directed mutagenesis mechanism, and then the alfalfa seeds are subjected to mutagenesis cultivation using a nutrient solution and a sodium chloride solution. Due to the adoption of stereoscopic batch directed mutagenesis, a considerable number of salt-tolerant alfalfa seeds can be easily obtained, and the best alfalfa seeds can be selected from the mutated salt-tolerant alfalfa seeds by cultivation in a subsequent test field. The present invention can place the plant pellets on each mutagenesis culture tray of the directional mutagenesis mechanism in batches through the air suction feeding mechanism, thereby reducing the working intensity of feeding; and the mutagenesis culture tray of the directional mutagenesis mechanism is arranged at intervals in the vertical direction, so that the alfalfa seeds are mutated and cultivated in batches in a three-dimensional manner, and according to the nutrients, oxygen, and salt required for the germination period of the alfalfa seeds, the nutrient solution, air, sodium chloride solution, etc. are supplied to each mutagenesis culture tray through the supply pipe system, thereby promoting the germination of the alfalfa seeds and directional salt tolerance; and a part of the nutrient solution, sodium chloride solution, etc. in the mutagenesis culture tray can be discharged through the discharge pipe system to avoid excessive nutrient solution, sodium chloride solution, etc., thereby ensuring the directional mutagenesis and germination of the alfalfa seeds. In summary, the present invention can efficiently directional mutagenesis and cultivate alfalfa, so that it has extremely high salt tolerance, reduces the difficulty of operation, and preferentially obtains salt-tolerant alfalfa seeds from the batch of mutated alfalfa, thereby ensuring the survival rate and income of the salt-tolerant alfalfa obtained on saline-alkali land. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0022] In the attached picture:

[0023] Figure 1 is a process flow chart of an embodiment of the present invention;

[0024] Figure 2 It is a structural schematic diagram of the directional mutagenesis mechanism when the transparent opening and closing cover is in a closed state according to an embodiment of the present invention;

[0025] Figure 3 It is a structural schematic diagram of the directional mutagenesis mechanism when the transparent opening and closing cover is in an open state according to an embodiment of the present invention;

[0026] Figure 4 This is a schematic diagram of the structure of the directed mutagenesis mechanism of an embodiment of the present invention after the transparent opening and closing cover is removed;

[0027] Figure 5 It is a schematic diagram of the structure of the base in the directed mutagenesis mechanism of an embodiment of the present invention;

[0028] Figure 6 It is a schematic diagram of the partial structure of the directed mutagenesis mechanism of an embodiment of the present invention;

[0029] Figure 7 It is a schematic diagram of the structure of a mutagenesis culture plate in a directed mutagenesis mechanism according to an embodiment of the present invention;

[0030] Figure 8 for Figure 7 A schematic diagram of another angle of the structure shown;

[0031] Fig. 9 It is a partial structural cross-sectional view of a mutagenesis culture dish in the directed mutagenesis mechanism of an embodiment of the present invention;

[0032] Fig.10 It is an axial structural cross-sectional view of a plant bucket in a mutagenesis culture tray according to an embodiment of the present invention;

[0033] Fig.11 This is an axial structural cross-sectional view of the vegetation bucket after being provided with the vegetation pills and the filter layer according to the embodiment of the present invention;

[0034] Fig.12 This is a schematic structural diagram of an air suction feeding mechanism according to an embodiment of the present invention;

[0035] Fig.13 It is a structural schematic diagram of the air suction type feeding mechanism from another angle according to an embodiment of the present invention;

[0036] Fig.14 It is a schematic structural diagram of a suction cup body in an air suction feeding mechanism according to an embodiment of the present invention;

[0037] Fig.15 It is an axial structural cross-sectional view of the connection between the upper end cover and the transfer arm in the air suction feeding mechanism of an embodiment of the present invention.

[0038] Labeled parts: 100-transparent opening and closing cover, 101-half cover body, 102-connecting ear, 103-exhaust joint, 104-exhaust control valve, 200-base, 201-seat body, 202-limiting edge, 203-first connecting joint, 204-second connecting joint, 205-drainage control valve, 300-LED lighting, 400-mutagenized culture tray, 401-tray body, 402-lower end wall , 403-connecting edge, 404-nutrient chamber, 405-supply joint, 406-discharge joint, 407-air guide hole, 408-vegetation part, 409-vegetation groove, 410-conducting hole, 411-transition part, 412-capillary part, 413-capillary channel, 414-inclined liquid suction port, 415-assembly ear, 416-fixing sleeve, 417-assembly port, 500-connecting rod, 600-supply Supply pipe system, 601-supply hose, 602-first joint pipe, 603-first control valve, 604-liquid supply pipe, 605-liquid supply control valve, 606-air supply pipe, 607-air supply control valve, 700-discharge pipe system, 701-discharge hose, 702-second joint pipe, 703-second control valve, 800-plant pill, 900-filter layer, 1000-air suction cup, 1001-suction cup Main body, 1002-adsorption groove, 1003-suction hole, 1004-air suction chamber, 1005-upper end cover, 1006-connecting column, 1007-first channel, 1100-lifting adjustment unit, 1101-vertical guide rail, 1102-linear slide, 1103-connecting seat, 1104-driving cylinder, 1105-adapting arm, 1106-air suction joint, 1107-second channel. DETAILED DESCRIPTION

[0039] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0040] The present invention discloses a method and device for EMS directed mutagenesis and screening of alfalfa. Figure 1 As shown, the following steps are included:

[0041] Step 1. Make the planting soil into spherical planting balls 800 in batches, plant alfalfa seeds soaked in the mutagen ethyl methanesulfonate in the planting balls 800, and the number of alfalfa seeds in each planting ball 800 is one;

[0042] Step 2. Use an air suction loading mechanism to place the plant pellets 800 in batches on each mutagenesis culture tray 400 of the directional mutagenesis mechanism;

[0043] Step 3. Supply the nutrient solution and sodium chloride solution to each mutagenesis culture tray 400 through the supply pipe system 600 of the directional mutagenesis mechanism, and control the illumination of each mutagenesis culture tray 400 at the same time;

[0044] Step 4. Transfer the germinated alfalfa seeds to the experimental field for cultivation;

[0045] Step 5. After the alfalfa matures, collect the alfalfa seeds with excellent mutations.

[0046] The working principle and advantages of the present invention are as follows: since the alfalfa seeds soaked in the mutagen ethyl methanesulfonate have extremely high multidirectional mutation ability, the alfalfa seeds can be subjected to directed mutagenesis; specifically, the soaked alfalfa seeds are placed in batches and layers in a plurality of mutagenesis culture trays 400 of a directed mutagenesis mechanism, and then the alfalfa seeds are subjected to mutagenesis cultivation using a nutrient solution and a sodium chloride solution. Due to the use of stereoscopic batch directed mutagenesis, a considerable number of salt-tolerant alfalfa seeds can be easily obtained, and the best alfalfa seeds can be selected from the mutated salt-tolerant alfalfa seeds by cultivation in a subsequent test field.

[0047] As a preferred embodiment of the present invention, the alfalfa seeds collected in step 5 are soaked with a mutagen ethyl methanesulfonate, and then, steps 1-step 5 are repeated to obtain salt-tolerant alfalfa seeds. In this way, the salt tolerance of the obtained alfalfa is better through secondary mutagenesis and cultivation. The present embodiment can also repeat steps 1-step 5 for many times, and the salt tolerance of the obtained alfalfa is also increased accordingly through multiple mutagenesis and cultivation, but the more the number of repetitions of mutagenesis, the more the lifting effect of the salt tolerance of alfalfa is gradually reduced; generally, it is best to repeat 2-3 times.

[0048] The present invention also discloses a device for the above-mentioned EMS directed mutagenesis and alfalfa screening method, such as Figure 2-15As shown, it includes a directional mutagenesis mechanism and an air-suction feeding mechanism. The directional mutagenesis mechanism is placed on the floor of a test bench or a test room, the air-suction feeding mechanism is arranged on one side of the directional mutagenesis mechanism, and the air-suction feeding mechanism is located at the opening and closing mouth of the transparent opening and closing cover 100, thereby facilitating the loading and unloading of the directional mutagenesis mechanism. The directional mutagenesis mechanism includes a base 200, a transparent opening and closing cover 100, a supply pipe system 600, an exhaust pipe system 700 and a plurality of mutagenesis culture trays 400, wherein the mutagenesis culture trays 400 are all arranged above the base 200, and the mutagenesis culture trays 400 are arranged at intervals in the vertical direction, the supply pipe system 600 and the exhaust pipe system 700 are respectively connected to each mutagenesis culture tray 400, the lower end of the transparent opening and closing cover 100 is arranged on the base 200, and the transparent opening and closing cover 100 is arranged outside the mutagenesis culture trays 400, and the transparent opening and closing cover 100 is covered with LED lighting lamps 300 to provide light to the alfalfa seeds that have just germinated in each mutagenesis culture tray 400. The working principle and advantages of the present invention are as follows: the present invention can place the plant pellets 800 in batches on each mutagenesis culture tray 400 of the directional mutagenesis mechanism through an air suction feeding mechanism, thereby reducing the working intensity of feeding; and the mutagenesis culture trays 400 of the directional mutagenesis mechanism are arranged at intervals in the vertical direction, so that alfalfa seeds can be mutated and cultivated in batches in a three-dimensional manner, and according to the nutrients, oxygen and salt required for mutagenesis during the germination period of the alfalfa seeds, nutrient solution, air, sodium chloride solution and the like are supplied to each mutagenesis culture tray 400 through a supply pipe system 600, thereby promoting the germination of the alfalfa seeds and directional salt tolerance; and a part of the nutrient solution, sodium chloride solution and the like in the mutagenesis culture tray 400 can be discharged through a discharge pipe system 700 to avoid excessive nutrient solution, sodium chloride solution and the like, thereby ensuring the directional mutagenesis and germination of the alfalfa seeds. In summary, the present invention can efficiently induce directed mutagenesis and cultivate alfalfa, so that it has extremely high salt tolerance, reduce the difficulty of operation, and selectively obtain salt-tolerant alfalfa seeds from a batch of induced alfalfa, thereby ensuring the survival rate and yield of the obtained salt-tolerant alfalfa on saline-alkali land.

[0049] As a preferred embodiment of the present invention, Figure 3 As shown, the transparent opening and closing cover 100 includes two half-cover bodies 101. A vertical pivot axis is installed on the base 200. The ends of the two half-cover bodies 101 that are close to each other are pivoted together through the pivot axis. A connecting ear 102 is constructed at the end of each half-cover body 101 that is away from the pivot axis. When the two half-cover bodies 101 are in a closed state, the connecting ears 102 on the two half-cover bodies 101 are aligned with each other, and the aligned connecting ears 102 are connected by a plurality of connecting bolts. In this embodiment, an exhaust joint 103 is constructed at the upper end of each half-cover body 101 for exhausting excess air, water vapor, etc. of the directional mutagenesis mechanism; an exhaust control valve 104 is installed on each exhaust joint 103 for adjusting the exhaust volume of the exhaust joint 103. Figure 5As shown, the base 200 includes a base body 201, and a limit edge 202 is constructed at the circumference of the upper end surface of the base body 201; when the transparent opening and closing cover 100 is in a closed state, the lower inner wall of each half cover body 101 is tightly attached to the outer peripheral wall at the corresponding position of the limit edge 202. In this embodiment, when the directional mutagenesis mechanism is loaded, the transparent opening and closing cover 100 is opened, and the air suction loading mechanism is used to place the plant pellets 800 in batches on each mutagenesis culture tray 400.

[0050] As a preferred embodiment of the present invention, Figure 7-9 As shown, the mutagenesis culture tray 400 includes a tray body 401, on the outer peripheral wall of which two assembly ears 415 are symmetrically constructed, each assembly ear 415 is constructed with a fixing sleeve 416, and an assembly port 417 is opened on the assembly ear 415. The tray body 401 of this embodiment is a hollow shell-like structure, wherein the hollow chamber is a nutrient chamber 404, and plant buckets are evenly constructed at the upper end of the tray body 401, and the lower end of each plant bucket extends to the lower part of the nutrient chamber 404. The middle part of the lower end wall 402 of the tray body 401 of this embodiment protrudes upward, and the lower end wall 402 gradually extends downwardly away from the middle part thereof, and a connecting edge 403 is formed at the outer edge of the lower end wall 402, and the connecting edge 403 is the lowest position of the lower end wall 402. A supply joint 405 and a discharge joint 406 are respectively constructed on the connecting edge 403, and the supply joint 405 and the discharge joint 406 are both connected to the nutrient chamber 404, so that the nutrient supply of the nutrient chamber 404 and the drainage of the nutrient chamber 404 are convenient. Two connecting rods 500 are fixed on the base 200, each connecting rod 500 extends upward in the vertical direction, and each connecting rod 500 is connected to the assembly ears 415 aligned on all the mutagenesis culture trays 400. Specifically, the connecting rod 500 passes through the aligned fixing sleeves 416 in turn, and the fixing sleeves 416 are threadedly connected with locking bolts, and the ends of the locking bolts are locked on the peripheral wall of the connecting rod 500, thereby achieving the fixing of the connecting rod 500 and the assembly ears 415. In this embodiment, the number of mutagenesis culture trays 400 can be increased or decreased according to demand, or the distance between adjacent mutagenesis culture trays 400 can be changed, thereby changing the number of alfalfa seeds to be mutated and cultivated.

[0051] As a preferred embodiment of the present invention, Figure 9-11As shown, the planting bucket includes a planting part 408, a transition part 411 and a capillary part 412 which are connected in sequence along the vertical direction downward. The planting part 408 is a hemispherical shell structure, and a planting groove 409 is formed in the planting part 408. The planting pill 800 is placed in the planting groove 409. This embodiment saves the process of laying the planting groove 409 with planting soil and subsequently planting alfalfa seeds in the planting groove 409. It only needs to place the seeds in the planting soil, and then prefabricate the planting soil into the planting pill 800; in this way, it is convenient for the air suction feeding mechanism to load and unload the mutation culture tray 400, and the planting pill 800 is adapted to the planting groove 409, and there is no need to adjust the angle of the planting pill 800 in the planting groove 409, that is, the planting pill 800 is spherical, and it can be assembled in the planting groove 409 at various angles, which saves the difficulty of operation and improves the planting efficiency. In this embodiment, a filter layer 900 is provided in the transition portion 411, and a capillary channel 413 is constructed in the capillary portion 412. The two ends of the capillary channel 413 are respectively connected to the transition portion 411 and the nutrient chamber 404. The lower end of the capillary portion 412 of this embodiment extends below the liquid level of the nutrient solution, sodium chloride solution, etc. in the nutrient chamber 404. Under the capillary action, the liquid enters the filter layer 900 through the capillary channel 413 and is continuously supplied to the plant pellet 800, so that the plant pellet 800 can absorb nutrients, salts, etc., which is convenient for the directional mutagenesis and germination of alfalfa seeds. In this embodiment, the capillary portion 412 can continuously and stably provide nutrients for alfalfa seeds, provide favorable conditions for the germination of alfalfa seeds, and avoid the occurrence of insufficient or excessive nutrient supply. In this embodiment, during the germination of alfalfa seeds, the demand for nutrients, salts, etc. of alfalfa seeds increases in certain special periods. In order to compensate for the insufficient supply of nutrients, salts, etc. in the capillary portion 412, the measures taken are to open a plurality of conduction holes 410 on the peripheral wall of the vegetation portion 408, and these conduction holes 410 are all connected to the nutrient chamber 404; at this time, the liquid level in the nutrient chamber 404 is raised to above the conduction holes 410, and the liquid can be directly supplied to the vegetation pill 800 through the conduction holes 410. In this embodiment, the arrangement of the plurality of conduction holes 410 on the vegetation portion 408 can be arranged along the vertical interval, so that according to the increase of the liquid level in the nutrient chamber 404, the liquid enters the vegetation groove 409 through the corresponding number of conduction holes 410 below the liquid level and is supplied to the vegetation pill 800, thereby adjusting the supply of nutrients, salts, etc. to the vegetation pill 800. In this embodiment, the lower end of the capillary portion 412 is formed with an inclined liquid suction port 414. Due to the setting of the inclined liquid suction port 414, the lower end of the capillary portion 412 is prevented from being blocked, and the liquid is ensured to smoothly enter the capillary channel 413 to avoid the situation of interruption of flow. In this embodiment, a plurality of air guide holes 407 are spaced apart on the upper end wall of the disk body 401, and these air guide holes 407 are all connected to the nutrient chamber 404.In this embodiment, air can be transported to the nutrient chamber 404 through the supply pipe system 600, a part of the air enters the planting groove 409 through the conducting hole 410, and the other part of the air is discharged from the nutrient chamber 404 through the air conducting hole 407, so that the transparent opening and closing cover 100 is filled with air, and the opening of the exhaust control valve 104 is controlled according to demand, so that the air volume in the transparent opening and closing cover 100 is within a predetermined range.

[0052] As a preferred embodiment of the present invention, Figure 4 , 6 As shown, the supply pipe system 600 includes a supply hose 601, which passes through the assembly ports 417 of the relatively aligned assembly ears 415 in sequence along the vertical direction. A plurality of first joint pipes 602 are constructed on the supply hose 601, and a first control valve 603 is installed on each first joint pipe 602. Each first joint pipe 602 is connected to a corresponding supply joint 405. A liquid supply pipe 604 is installed at the inlet end of the supply hose 601, and an air supply pipe 606 is installed on the liquid supply pipe 604. A liquid supply control valve 605 and an air supply control valve 607 are installed on the liquid supply pipe 604 and the air supply pipe 606, respectively. Liquids such as nutrient solution and sodium chloride solution are supplied to the supply hose 601 through the liquid supply pipe 604, and then are evenly distributed to each supply joint 405 through each first joint pipe 602, and then evenly supplied to each mutagenesis culture tray 400. The gas is supplied to the supply hose 601 through the gas supply pipe 606 , and then evenly distributed to each supply joint 405 through each first joint pipe 602 , and further evenly supplied to each mutagenesis culture tray 400 .

[0053] As a preferred embodiment of the present invention, Figure 4-6 As shown, the discharge pipe system 700 includes a discharge hose 701, which passes through the assembly openings 417 of the relatively aligned assembly ears 415 in sequence along the vertical direction. A plurality of second joint pipes 702 are constructed on the discharge hose 701, and a second control valve 703 is installed on each second joint pipe 702. Each second joint pipe 702 is connected to a corresponding discharge joint 406. A discharge channel is provided in the base 200, and a first connection joint 203 and a second connection joint 204 are respectively constructed on the base 200 and located at both ends of the discharge channel. The first connection joint 203 is connected to the lower end of the discharge hose 701, and a discharge control valve 205 is installed on the second connection joint 204. In this embodiment, the liquid in the nutrient chamber 404 is discharged through the discharge joint 406, and enters the discharge hose 701 through the second joint pipe 702, and then is discharged through the first connection joint 203, the discharge channel and the second connection joint 204 in sequence.

[0054] As a preferred embodiment of the present invention, Figure 12-15As shown, the air suction feeding mechanism includes a lifting adjustment unit 1100 and an air suction cup 1000, and the air suction cup 1000 is installed at one end of the lifting adjustment unit 1100. The air suction cup 1000 of this embodiment includes a suction cup body 1001 having an air suction chamber 1004, and an upper end cover 1005 is detachably connected to the upper end of the suction cup body 1001. A plurality of hemispherical suction grooves 1002 are constructed at the lower end of the suction cup body 1001, and an air suction hole 1003 is opened on each of the suction grooves 1002, and the air suction hole 1003 is connected to the air suction chamber 1004. A connecting column 1006 is constructed on the upper end cover 1005, and the connecting column 1006 is connected to the transfer arm 1105. A first channel 1007 and a second channel 1107 are respectively provided on the connecting column 1006 and the transfer arm 1105. The first channel 1007 and the second channel 1107 are interconnected. The first channel 1007 is connected to the air suction chamber 1004. An air suction joint 1106 is configured on the transfer arm 1105. The air suction joint 1106 is interconnected with the second channel 1107. In this embodiment, the air suction joint 1106 is sucked to suck the plant pellets 800 in the adsorption tank 1002, and the plant pellets 800 are transferred through the lifting adjustment unit 1100. The lifting adjustment unit 1100 of this embodiment includes a connecting seat 1103, a driving cylinder 1104, a linear slide 1102, and a vertical guide rail 1101. The driving cylinder 1104 is connected between the transfer arm 1105 and the connection seat 1103, the linear slide 1102 is installed on the connection seat 1103, and the linear slide 1102 is assembled on the vertical guide rail 1101. In this embodiment, the linear slide 1102 moves on the vertical guide rail 1101 to feed the mutagenesis culture trays 400 at different heights, and the driving cylinder 1104 drives the air suction cup 1000 to extend above the mutagenesis culture tray 400, so that the plant pellet 800 can be smoothly placed in the plant bucket of the mutagenesis culture tray 400.

[0055] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein with equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the scope of protection of the claims of the present invention.

Claims

1. A device for EMS directed mutagenesis and screening of alfalfa, characterized in that: The invention comprises a directional mutagenesis mechanism arranged on a test bench or a laboratory floor, wherein the directional mutagenesis mechanism comprises a plurality of mutagenesis culture plates installed on a base, wherein the mutagenesis culture plates are arranged at intervals in a vertical direction, wherein a supply pipe system and an exhaust pipe system are respectively connected to each mutagenesis culture plate, and a transparent opening and closing cover for covering the mutagenesis culture plates is arranged on the base, wherein the transparent opening and closing cover is covered with LED lighting lamps; an air suction feeding mechanism is arranged on one side of the directional mutagenesis mechanism and is located at an opening and closing opening of the transparent opening and closing cover; The mutagenesis culture tray comprises a tray body with a nutrient cavity, and a planting bucket is evenly constructed at the upper end of the tray body, and the lower end of each planting bucket extends to the lower part of the nutrient cavity. A supply joint and a discharge joint are respectively constructed on the lower end wall of the tray body, and the supply joint and the discharge joint are both connected to the nutrient cavity. The tray body is connected to two connecting rods, and the lower ends of the two connecting rods are fixed to the base; The plant bucket comprises a plant part, a transition part and a capillary part which are connected in sequence along the vertical direction downward, the plant part is a hemispherical shell structure, a plant groove is formed in the plant part, the plant balls are placed in the plant groove, a filter layer is arranged in the transition part, a capillary channel connected with the transition part is constructed in the capillary part, and the capillary channel is connected with the nutrient chamber; The air suction feeding mechanism includes an air suction cup installed at one end of the lifting adjustment unit, the air suction cup includes a suction cup body with an air suction chamber, the upper end of the suction cup body is detachably connected with an upper end cover, a plurality of hemispherical adsorption grooves are constructed at the lower end of the suction cup body, each of the adsorption grooves is provided with an air suction hole connected to the air suction chamber, a connecting column connected to the transfer arm is constructed on the upper end cover, a first channel and a second channel connected to each other are respectively opened on the connecting column and the transfer arm, the first channel is connected to the air suction chamber, and an air suction joint connected to the second channel is constructed on the transfer arm.

2. The device for EMS directed mutagenesis and screening of alfalfa according to claim 1, characterized in that: A plurality of conducting holes are provided on the peripheral wall of the vegetation part, and these conducting holes are all connected to the nutrient cavity. An inclined liquid suction port is formed at the lower end of the capillary part, and a plurality of air guide holes are provided at intervals on the upper end wall of the disk body, and these air guide holes are all connected to the nutrient cavity.

3. The device for EMS directed mutagenesis and screening of alfalfa according to claim 1, characterized in that: The supply pipe system includes a supply hose constructed with multiple first joint pipes, a first control valve is installed on each of the first joint pipes, each first joint pipe is connected to a corresponding supply joint, a liquid supply pipe is installed at the inlet end of the supply hose, an air supply pipe is installed on the liquid supply pipe, and a liquid supply control valve and an air supply control valve are installed on the liquid supply pipe and the air supply pipe, respectively.

4. The device for EMS directed mutagenesis and screening of alfalfa according to claim 1, characterized in that: The discharge pipe system includes a discharge hose constructed with multiple second joint pipes, a second control valve is installed on each of the second joint pipes, each second joint pipe is connected to a corresponding discharge joint, a discharge channel is opened in the base, and a first connecting joint and a second connecting joint are respectively constructed on the base and located at both ends of the discharge channel, the first connecting joint is connected to the lower end of the discharge hose, and a discharge control valve is installed on the second connecting joint.

5. The device for EMS directed mutagenesis and screening of alfalfa according to claim 1, characterized in that: The lifting adjustment unit comprises a driving cylinder connected between a transfer arm and a connecting seat, a linear slide is installed on the connecting seat, and the linear slide is assembled on a vertical guide rail.

Images