Lectin coating agent for strong gluten wheat and coating equipment

Abstract

The invention relates to the technical field of strong gluten wheat lectin coating, in particular to a lectin coating agent and coating equipment for strong gluten wheat, the lectin coating agent comprises a sporocarp, a buffer solution, a biological fertilizer, a microbial agent and a filler, the sporocarp is a macro fungus edible fungus, and the buffer solution is a phosphate buffer solution; viscosity adjustment convenience of a coating agent is improved, stress resistance and disease resistance of seeds are improved, wheat coating quality of equipment is improved, wheat coating pretreatment convenience is improved, and wheat germination rate is increased.

Description

Technical Field

[0001] The invention relates to the technical field of lectin coating for strong-gluten wheat, in particular to a lectin coating agent and coating equipment for strong-gluten wheat. Background Art

[0002] With the continuous development of agricultural science and technology, seed treatment technology has been significantly improved. Among them, lectin coating agents, as an advanced seed treatment technology, have been widely used in wheat planting.

[0003] As in the patent with prior art authorization announcement number CN207911285U, the utility model relates to a seed coating machine, including a stirring tank, a bracket and a supporting wheel, the stirring tank and the supporting wheel combination are respectively provided with a ring gear and a gear, the ring gear and the gear are meshed with each other, the seed coating machine also includes a discharging system and a spraying system, the discharging system includes a material box, the material box is supported by legs, and a feed port b and a discharging hopper are respectively provided at the top and bottom of the material box, the spraying system includes a stirring motor, a stirring blade, a pump body and a medicine storage tank, and the outlet end of the pump body is connected to the feed port a of the stirring tank through a sprayer.

[0004] However, during the use of this equipment, it was found that the equipment was not convenient for screening and dust removal before seed coating. The germination rate of wheat seeds with insufficient grains was affected after coating, and the cost waste of coating was increased, which reduced the quality of wheat coating. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a lectin coating agent and coating equipment for strong-gluten wheat, which can improve the convenience of adjusting the viscosity of the coating agent, improve the stress resistance and disease resistance of seeds, improve the quality of wheat coating by equipment, improve the convenience of wheat coating pretreatment, and improve the germination rate of wheat.

[0006] The invention discloses a lectin coating agent for strong-gluten wheat, comprising a fruiting body, a buffer, a biological fertilizer, a microbial agent and a filler, wherein the fruiting body is a large edible fungus, and the buffer is a phosphate buffer; the biological fertilizer, the microbial agent and the filler are mixed with the fruiting body, wherein the biological fertilizer is used to provide the nutrition required for seed growth and improve the seed germination rate, the microbial agent improves the seed germination speed by regulating the physiological metabolism during seed growth, and the viscosity adjustment convenience of the coating agent is improved by mixing the filler, so that the lectin coating agent forms a uniform and dense coating layer, which covers the surface of the strong-gluten wheat seeds, improves the stress resistance and disease resistance of the seeds, and provides the nutrition and regulator required for seed growth, so as to promote the germination and growth of the seeds.

[0007] Preferably, the large edible fungi include Agaricus bisporus. As a large edible fungi, Agaricus bisporus has a high lectin content in its fruiting body. By using Agaricus bisporus as a raw material, the preparation convenience of the lectin coating agent is improved.

[0008] Preferably, the preparation method of the lectin coating agent comprises the following steps:

[0009] S1. Extract lectins from multiple Agaricus bisporus fruiting bodies cultivated by the research group, draw standard curves, determine the lectin content, and statistically analyze and screen out strains with high lectin content;

[0010] S2, placing the Agaricus bisporus fruiting bodies with high lectin content in a food drying machine at 60° C. and drying for 24 hours to prepare a dry product;

[0011] S3, crush the dry product with a grinder and filter it with a 35-mesh sieve, weigh and record the crushed Agaricus bisporus dry product after screening, mix it with 20 mM phosphate buffer at a ratio of 1:10, add it to a 50 mL centrifuge tube, shake well and let it stand and soak at 4 ° C for 12 hours;

[0012] S4, centrifuge the soaking liquid in a refrigerated centrifuge at 4°C, 12000r / min for 22min, take the supernatant and filter the supernatant solids, centrifuge the filtered supernatant again at 4°C, 12000r / min for 22min, filter the supernatant, slowly add (NH4)2SO4 to the filtered solution in an ice water bath to precipitate, saturation reaches 40%, slowly stir at 4°C for 2h, centrifuge at 12000r / min for 30min, take the supernatant and discard the precipitate, take the supernatant and slowly add (NH4)2SO4 again to saturation reach 60%, slowly stir at 4°C for 4h, finally centrifuge the stirred and mixed sample at 4°C, 12000r / min for 30min, and discard the supernatant;

[0013] S5, dissolve the precipitate base liquid with 20mM PBS buffer and pour it into a 35mm diameter dialysis bag for dialyzed desalination, and soak the dialysis bag in a solution of 0.2M PBS phosphate buffer diluted 1:10 with pure water, add magnetic beads and stir the solution at low temperature on a magnetic stirrer for 10h, then put the dialysate into a centrifuge tube at 4℃, 12000r / min for 30min to obtain the precipitate, and then dissolve and concentrate the precipitate with 20mM PBS buffer to obtain a crude sample solution;

[0014] S6, placing the crude sample solution in a freeze dryer for freeze drying to obtain a lyophilized crude lectin product, and then dissolving the lyophilized crude product in 20 mM Tris-HCl buffer (pH=7.8) to obtain Agaricus bisporus lectin;

[0015] S7. Evenly mix the Agaricus bisporus lectin with the biological fertilizer, the microbial agent and the filler to obtain the lectin coating agent.

[0016] Preferably, a lectin coating device for strong gluten wheat comprises a driving device, a collecting device, a heating device, a spraying device, a first box, a screen cylinder, blades, a conical cylinder, a baffle, a conveying box and a ring body, wherein an air inlet valve is arranged on the first box, a take-in and put-out port is arranged on the right side wall of the first box, a through hole is arranged on the left end of the first box, the screen cylinder is rotatably installed in the first box through the driving device, and the opening of the screen cylinder is communicated with the take-in and put-out port of the first box, a plurality of groups of blades are circumferentially installed on the inner side wall of the screen cylinder, the conical cylinder extends into the interior of the screen cylinder through the through hole of the first box, and a through hole is arranged on the upper half of the conical cylinder There are screen holes, the baffle is installed at the right end of the conical cylinder, the ring body is mounted on the left side wall of the conical cylinder, the conveying box is connected with the left end of the conical cylinder, the conveying box is installed on the collecting device, the collecting device is used to drive the conveying box to move left and right, the collecting device is used to collect the screened wheat, and the collecting device is used to remove dust from the wheat, the spraying device is connected with the screen cylinder, the spraying device is used to spray the coating agent, the heating device is connected with the first box body, and the heating device is used to dry the coated wheat; put the wheat into the screen cylinder through the first box body taking and putting port, and then drive the conveying box to move right through the collecting device, so that The conveying box drives the conical cylinder to extend into the screen cylinder, so that the baffle closes the access opening of the first box body, and the ring body closes the through hole between the conical cylinder and the first box body. The driving device drives the screen cylinder to rotate, so that the screen cylinder scoops up the wheat through multiple sets of blades and throws it down. The thrown wheat falls to the top of the conical cylinder, and the unfull small grains of wheat are screened through the screen holes in the upper half of the conical cylinder. The screened small grains of wheat are conveyed to the conveying box through the inside of the conical cylinder, and then the screened wheat is conveyed to the collecting device through the conveying box. At the same time, the collecting device inhales air in the conveying box, so that outdoor air passes through the first An air inlet valve of a box body enters into the screen cylinder, thereby removing dust from the wheat. After the wheat screening and dust removal are completed, the conveying box drives the conical cylinder to move to the left and out of the outside of the screen cylinder. At this time, the baffle closes the through hole of the first box body and opens the first box body access port, and then extends into the screen cylinder through the spraying device, so that the spraying device coats the wheat with full particles in the screen cylinder. When the wheat coating is completed, the screen cylinder is rotated in the opposite direction so that multiple groups of blades discharge the wheat outward through the first box body access port, thereby improving the quality of wheat coating of the equipment, improving the convenience of wheat coating pre-treatment, and improving the wheat germination rate.

[0017] Preferably, the collecting device comprises a power device, a dust suction device, a second box, a guide platform, multiple groups of telescopic rods, multiple groups of springs, a support net, a frame, an electric cylinder, a vibration motor, a first nozzle and a closed door. The second box is slidably mounted on the guide platform left and right by the power device, the conveying box is connected and arranged at the top of the second box, the multiple groups of telescopic rods are all mounted on the bottom of the inner wall of the second box, the multiple groups of springs are respectively fitted on the outer walls of the multiple groups of telescopic rods, the frame is mounted on the top of the multiple groups of telescopic rods, the left end of the support net is rotatably mounted on the frame, the fixed end of the electric cylinder is rotatably mounted on the outer wall of the frame, the moving end of the electric cylinder is rotatably connected to the left part of the support net, the vibration motor is mounted on the outer wall of the frame, the first nozzle is mounted on the top of the second box, and the first nozzle is connected to the heating device, the outer wall of the second box is provided with a discharge port, the closed door is installed at the discharge port, the dust suction device is connected to the conveying box, and the dust suction device is used to suck air into the conveying box; the conveying box will sieve The small-particle wheat is transported and discharged into the second box body. The wheat entering the second box body falls to the top of the supporting net. The vibration motor is turned on to drive the frame to vibrate, and the frame drives the supporting net to vibrate, so that the wheat is evenly spread on the top of the supporting net. When the wheat in the screen cylinder is sprayed and coated, hot air is blown into the first box body through the heating device, and the hot air passes through the inside of the screen cylinder to dry the coated wheat. Then the hot air is transported to the inside of the first nozzle. Since the hot air contains evaporative products of lectin coating agent, the hot air is blown to the wheat at the top of the supporting net, so that the wheat at the top of the supporting net intercepts and filters the lectin coating agent in the hot air, and then the hot air flows back to the heating device, thereby improving the convenience of hot air circulation and lectin recovery. The closed door is opened and the right part of the supporting net is pushed upward by the electric cylinder to discharge the wheat at the top of the supporting net, thereby improving the convenience of equipment use.

[0018] Preferably, the heating device includes a third box, an electric heating tube, a first fan, a first hose and a second hose. The third box is connected and arranged at the lower part of the outer side wall of the first box, the electric heating tube is installed inside the third box, the output end of the first fan is connected with the third box, the input end of the first fan is connected with the first hose, the input end of the first hose is connected with the second box, the input end of the second hose is connected and arranged at the top of the first box, and the output end of the second hose is connected with the first nozzle. The first fan is used to blow air into the third box, and the electric heating tube heats the air in the third box at the same time, so that the hot air enters the first box, and then the hot air flows through the screen cylinder, so that the hot air dries the coated wheat, and then the hot air is recovered and transported to the first nozzle through the second hose, so that the first nozzle blows the hot air to the wheat on the top of the supporting net, so that the wheat collects the lectin, and then the hot air flows back to the first fan through the first hose to realize the recycling of the hot air.

[0019] Preferably, the spraying device includes an electric rotating table, a support arm, a cover body, a second nozzle, a first gear, a first driving motor and a rack. The electric rotating table is installed on the top of the first box body, the support arm is slidably installed on the rotating end of the electric rotating table left and right, the cover body is installed on the bottom of the support arm, the second nozzle is installed on the outer side wall of the cover body, the first gear is rotatably installed on the inner side wall of the rotating end of the electric rotating table, the first driving motor is installed on the outer side wall of the rotating end of the electric rotating table, the output end of the first driving motor is connected to the first gear, the rack is arranged on the outer side wall of the support arm, and the rack is connected to the first gear. a gear meshes; when the conical cylinder moves to the left and out of the outside of the screen cylinder, the first gear is driven to rotate by the first driving motor, so that the first gear drives the support arm to move to the left through the meshing with the rack, so that the cover body is installed at the first box body taking and releasing port, and the second nozzle extends into the screen cylinder at the same time, and then the lectin coating agent is transported to the second nozzle, so that the second nozzle sprays the coating agent onto the wheat, and the supporting arm is driven to swing by the rotating end of the electric turntable, so as to facilitate the cover body to move to the side of the first box body, thereby improving the convenience of taking and placing the wheat in the screen cylinder.

[0020] Preferably, the dust suction device includes a collecting box, a second fan and a filter. The collecting box is connected and arranged on the outer side wall of the second driving motor, the second fan is connected and arranged on the outer side wall of the collecting box, and the filter is arranged on the input end of the second fan. The second fan sucks air into the collecting box, so that the collecting box collects dust in the wheat, thereby improving the convenience of dust removal treatment of the wheat.

[0021] Preferably, the driving device includes a second driving motor, a second gear and a gear ring, the second driving motor is mounted on the outer wall of the first box body, the second gear is mounted on the output end of the second driving motor, the gear ring is mounted on the outer wall of the screen cylinder, and the gear ring is meshed with the second gear; the second driving motor drives the second gear to rotate, and the second gear drives the screen cylinder to rotate through the meshing with the gear ring, thereby improving the convenience of the screen cylinder for mixing wheat.

[0022] Preferably, the power device includes a screw and a third drive motor, the screw is rotatably installed on the inner wall of the guide platform, the bottom of the second box is screwed on the screw, the third drive motor is installed on the outer wall of the guide platform, and the output end of the third drive motor is connected to the screw; the screw is driven to rotate by the third drive motor, so that the screw drives the second box to move left and right, thereby improving the convenience of movement and adjustment of the conical cylinder.

[0023] Compared with the prior art, the present invention has the following beneficial effects: by mixing biological fertilizer, microbial agent and filler with fruiting body, the biological fertilizer is used to provide nutrition required for seed growth and improve seed germination rate, the microbial agent improves seed germination speed by regulating physiological metabolism in the seed growth process, and by mixing filler, the viscosity adjustment convenience of coating agent is improved, so that lectin coating agent forms a uniform and dense coating layer, which covers the surface of strong gluten wheat seeds, improves the stress resistance and disease resistance of seeds, and at the same time provides nutrition and regulator required for seed growth, and promotes seed germination and growth. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the axonometric structure of the present invention;

[0025] Figure 2 It is a schematic diagram of the axonometric structure of the connection between the second box body and the guide platform, etc.;

[0026] Figure 3 It is a schematic diagram of the axonometric partial structure of the connection between the first box body and the screen cylinder, etc.;

[0027] Figure 4 It is a schematic diagram of the axonometric structure of the connection between the conical cylinder and the baffle, etc.;

[0028] Figure 5 is a schematic diagram of a partial structure of the connection between the first nozzle and the second hose, etc. from a side view;

[0029] Figure 6 is a side view structural diagram of the connection between the second box body and the telescopic rod, etc.;

[0030] Figure 7 It is a schematic diagram of the axonometric structure of the connection between the guide table and the lead screw;

[0031] Figure 8 It is an axonometric structural diagram of the connection between the third box and the first fan, etc.;

[0032] Fig. 9 It is a schematic diagram of the axonometric partial structure of the connection between the support arm and the cover body;

[0033] Fig.10 It is a schematic diagram of the axonometric structure of the connection between the conical cylinder and the ring body;

[0034] Fig.11 It is a schematic diagram of the axonometric structure of the connection between the second box body and the conveying box, etc.;

[0035] Fig.12 It is an axonometric diagram of the local structure connecting the support grid and the frame.

[0036] Markings in the attached drawings: 101, first box; 102, screen cylinder; 103, blades; 104, conical cylinder; 105, baffle; 106, conveying box; 107, ring body; 201, second box; 202, guide platform; 203, telescopic rod; 204, spring; 205, support net; 206, frame; 207, electric cylinder; 208, vibration motor; 209, first nozzle; 210, closed door; 301, third box; 302, electric heating pipe; 303, first fan; 304, first hose; 305, second hose; 401, electric rotating table; 402, support arm; 403, cover body; 404, second nozzle; 405, first gear; 406, first drive motor; 407, rack; 501, collection box; 502, second fan; 503, filter; 601, second drive motor; 602, second gear; 603, gear ring; 701, lead screw; 702, third drive motor. DETAILED DESCRIPTION

[0037] In order to facilitate understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0038] Example 1

[0039] The lectin coating agent for strong gluten wheat of the present invention comprises a fruiting body, a buffer, a biological fertilizer, a microbial agent and a filler, wherein the fruiting body is a large edible fungus, and the buffer is a phosphate buffer;

[0040] The macro-fungi edible mushrooms include Agaricus bisporus;

[0041] The preparation method of the lectin coating agent comprises the following steps:

[0042] S1. Extract lectins from multiple Agaricus bisporus fruiting bodies cultivated by the research group, draw standard curves, determine the lectin content, and statistically analyze and screen out strains with high lectin content;

[0043] S2, placing the Agaricus bisporus fruiting bodies with high lectin content in a food drying machine at 60° C. and drying for 24 hours to prepare a dry product;

[0044] S3, crush the dry product with a grinder and filter it with a 35-mesh sieve, weigh and record the crushed Agaricus bisporus dry product after screening, mix it with 20 mM phosphate buffer at a ratio of 1:10, add it to a 50 mL centrifuge tube, shake well and let it stand and soak at 4 ° C for 12 hours;

[0045] S4, centrifuge the soaking liquid in a refrigerated centrifuge at 4°C, 12000r / min for 22min, take the supernatant and filter the supernatant solids, centrifuge the filtered supernatant again at 4°C, 12000r / min for 22min, filter the supernatant, slowly add (NH4)2SO4 to the filtered solution in an ice water bath to precipitate, saturation reaches 40%, slowly stir at 4°C for 2h, centrifuge at 12000r / min for 30min, take the supernatant and discard the precipitate, take the supernatant and slowly add (NH4)2SO4 again to saturation reach 60%, slowly stir at 4°C for 4h, finally centrifuge the stirred and mixed sample at 4°C, 12000r / min for 30min, and discard the supernatant;

[0046] S5, dissolve the precipitate base liquid with 20mM PBS buffer and pour it into a 35mm diameter dialysis bag for dialyzed desalination, and soak the dialysis bag in a solution of 0.2M PBS phosphate buffer diluted 1:10 with pure water, add magnetic beads and stir the solution at low temperature on a magnetic stirrer for 10h, then put the dialysate into a centrifuge tube at 4℃, 12000r / min for 30min to obtain the precipitate, and then dissolve and concentrate the precipitate with 20mM PBS buffer to obtain a crude sample solution;

[0047] S6, placing the crude sample solution in a freeze dryer for freeze drying to obtain a lyophilized crude lectin product, and then dissolving the lyophilized crude product in 20 mM Tris-HCl buffer (pH=7.8) to obtain Agaricus bisporus lectin;

[0048] S7, uniformly mixing the Agaricus bisporus lectin with the biological fertilizer, the microbial agent and the filler to obtain a lectin coating agent;

[0049] In this embodiment, biological fertilizer, microbial agent and filler are mixed with fruiting bodies, wherein the biological fertilizer is used to provide the nutrients required for seed growth and improve the seed germination rate, the microbial agent increases the seed germination rate by regulating the physiological metabolism during seed growth, and the filler is mixed to improve the convenience of adjusting the viscosity of the coating agent, so that the lectin coating agent forms a uniform and dense coating layer, which covers the surface of the strong gluten wheat seeds, thereby improving the seed's stress resistance and disease resistance, and at the same time providing the nutrients and regulators required for seed growth, thereby promoting seed germination and growth.

[0050] Example 2

[0051] On the basis of Example 1, a lectin coating device for strong gluten wheat of the present invention comprises a driving device, a collecting device, a heating device, a spraying device, a first box body 101, a screen cylinder 102, blades 103, a conical cylinder 104, a baffle 105, a conveying box 106 and a ring body 107, wherein the first box body 101 is provided with an air inlet valve, the right side wall of the first box body 101 is provided with a take-in and put-out port, the left end of the first box body 101 is provided with a through hole, the screen cylinder 102 is rotatably installed in the first box body 101 by the driving device, and the opening of the screen cylinder 102 is communicated with the take-in and put-out port of the first box body 101, a plurality of groups of blades 103 are circumferentially installed on the inner wall of the screen cylinder 102, and the conical cylinder 104 is provided with a baffle 105. The conveying box 106 and the ring body 107 are provided with an air inlet valve. The cylinder 104 extends into the screen cylinder 102 through the through hole of the first box body 101, and the upper half of the conical cylinder 104 is provided with screen holes, the baffle 105 is installed at the right end of the conical cylinder 104, the ring body 107 is sleeved on the left side wall of the conical cylinder 104, the conveying box 106 is connected with the left end of the conical cylinder 104, the conveying box 106 is installed on the collecting device, the collecting device is used to drive the conveying box 106 to move left and right, the collecting device is used to collect the screened wheat, and the collecting device is used to remove dust from the wheat, the spraying device is communicated with the screen cylinder 102, the spraying device is used to spray the coating agent, the heating device is communicated with the first box body 101, and the heating device is used to dry the coated wheat;

[0052] The collecting device includes a power device, a dust collecting device, a second box 201, a guide platform 202, multiple sets of telescopic rods 203, multiple sets of springs 204, a supporting net 205, a frame 206, an electric cylinder 207, a vibration motor 208, a first nozzle 209 and a closed door 210. The second box 201 is slidably mounted on the guide platform 202 by the power device, the conveying box 106 is connected and arranged at the top of the second box 201, the multiple sets of telescopic rods 203 are all installed at the bottom of the inner wall of the second box 201, the multiple sets of springs 204 are respectively fitted on the outer walls of the multiple sets of telescopic rods 203, and the frame 206 is provided with a plurality of springs 204. Installed on the top of multiple sets of telescopic rods 203, the left end of the support net 205 is rotatably installed on the frame 206, the fixed end of the electric cylinder 207 is rotatably installed on the outer wall of the frame 206, the movable end of the electric cylinder 207 is rotatably connected to the left part of the support net 205, the vibration motor 208 is installed on the outer wall of the frame 206, the first nozzle 209 is installed on the top of the second box 201, and the first nozzle 209 is connected to the heating device, the outer wall of the second box 201 is provided with a discharge port, and the closed door 210 is installed at the discharge port, the dust suction device is connected to the conveying box 106, and the dust suction device is used to suck air in the conveying box 106;

[0053] The heating device comprises a third box 301, an electric heating pipe 302, a first fan 303, a first hose 304 and a second hose 305. The third box 301 is arranged at the lower part of the outer wall of the first box 101, the electric heating pipe 302 is installed inside the third box 301, the output end of the first fan 303 is connected to the inside of the third box 301, the input end of the first fan 303 is connected to the first hose 304, the input end of the first hose 304 is connected to the inside of the second box 201, the input end of the second hose 305 is arranged at the top of the first box 101, and the output end of the second hose 305 is connected to the first nozzle 209;

[0054] The spraying device includes an electric rotating platform 401, a support arm 402, a cover body 403, a second nozzle 404, a first gear 405, a first drive motor 406 and a rack 407. The electric rotating platform 401 is installed at the top of the first box body 101, the support arm 402 is slidably installed on the rotating end of the electric rotating platform 401, the cover body 403 is installed at the bottom of the support arm 402, the second nozzle 404 is installed on the outer wall of the cover body 403, the first gear 405 is rotatably installed on the inner wall of the rotating end of the electric rotating platform 401, the first drive motor 406 is installed on the outer wall of the rotating end of the electric rotating platform 401, the output end of the first drive motor 406 is connected to the first gear 405, the rack 407 is arranged on the outer wall of the support arm 402, and the rack 407 is meshed with the first gear 405;

[0055] The dust collecting device includes a collection box 501, a second fan 502 and a filter 503, the collection box 501 is connected and arranged on the outer side wall of the second driving motor 601, the second fan 502 is connected and arranged on the outer side wall of the collection box 501, and the filter 503 is arranged on the input end of the second fan 502;

[0056] The driving device includes a second driving motor 601, a second gear 602 and a gear ring 603. The second driving motor 601 is mounted on the outer wall of the first box body 101, the second gear 602 is mounted on the output end of the second driving motor 601, and the gear ring 603 is mounted on the outer wall of the screen cylinder 102. The gear ring 603 is meshed with the second gear 602.

[0057] The power device includes a lead screw 701 and a third drive motor 702. The lead screw 701 is rotatably mounted on the inner wall of the guide platform 202. The bottom of the second box 201 is screwed on the lead screw 701. The third drive motor 702 is mounted on the outer wall of the guide platform 202. The output end of the third drive motor 702 is connected to the lead screw 701.

[0058] In the present embodiment, Agaricus bisporus is a large edible fungus with a high lectin content in its fruiting body. Agaricus bisporus is used as a raw material to improve the convenience of preparing the lectin coating agent. Wheat is put into the screen cylinder 102 through the access port of the first box body 101, and then the conveying box 106 is driven to move to the right by the collecting device, so that the conveying box 106 drives the conical cylinder 104 to extend into the screen cylinder 102, so that the baffle 105 closes the access port of the first box body 101, and the ring body 107 closes the through hole between the conical cylinder 104 and the first box body 101, and the screen cylinder 102 is driven to rotate by the driving device, so that the screen cylinder 102 scoops up the wheat through the multiple sets of blades 103 and throws it downward, and the thrown wheat falls to the top of the conical cylinder 104, and the unfull small grains of wheat are screened through the screen holes in the upper half of the conical cylinder 104, and the screened small grains of wheat pass through the conical cylinder 104. The wheat is transported to the conveying box 106, and then the screened wheat is transported to the collecting device through the conveying box 106. At the same time, the collecting device sucks air into the conveying box 106, so that the outdoor air enters the screen cylinder 102 through the air inlet valve of the first box body 101, so that the wheat is dusted. After the wheat screening and dust removal are completed, the conveying box 106 drives the conical cylinder 104 to move left out of the screen cylinder 102. At this time, the baffle 105 closes the through hole of the first box body 101 and opens the first box body 101 access port, and then extends into the screen cylinder 102 through the spraying device, so that the spraying device coats the wheat with full particles in the screen cylinder 102. When the wheat coating is completed, the screen cylinder 102 is rotated in the opposite direction, so that the multiple sets of blades 103 discharge the wheat through the access port of the first box body 101, thereby improving the quality of wheat coating by the equipment, improving the convenience of wheat coating pre-treatment, and improving the wheat germination rate.

[0059] like Figures 1 to 12As shown, a lectin coating device for strong gluten wheat of the present invention, when working, puts the wheat into the screen cylinder 102 through the access port of the first box body 101, and then drives the conveying box 106 to move to the right through the collecting device, so that the conveying box 106 drives the conical cylinder 104 to extend into the screen cylinder 102, so that the baffle 105 closes the access port of the first box body 101, and at the same time the ring body 107 closes the through hole between the conical cylinder 104 and the first box body 101, and drives the screen cylinder 102 to rotate through the driving device, so that the screen cylinder 102 scoops up the wheat through the multiple sets of blades 103 and throws it downward, and the thrown wheat falls to the top of the conical cylinder 104, and the unfull small-grain wheat is screened through the screen holes in the upper half of the conical cylinder 104, and the screened small-grain wheat passes through the conical cylinder 104. The screened wheat is transported to the conveying box 106, and then the screened wheat is transported to the collecting device through the conveying box 106. At the same time, the collecting device sucks air into the conveying box 106, so that the outdoor air enters the screen cylinder 102 through the air intake valve of the first box body 101, so that the wheat is dusted. After the wheat screening and dust removal are completed, the conveying box 106 drives the conical cylinder 104 to move to the left and out of the outside of the screen cylinder 102. At this time, the baffle 105 closes the through hole of the first box body 101 and opens the first box body 101 access port, and then extends into the screen cylinder 102 through the spraying device, so that the spraying device coats the wheat with full particles in the screen cylinder 102. When the wheat coating is completed, the screen cylinder 102 is rotated in the opposite direction, so that the multiple sets of blades 103 discharge the wheat through the access port of the first box body 101.

[0060] The main functions achieved by the present invention are: improving the convenience of screening and dust removal before wheat coating, improving the quality of wheat coating, improving the stress resistance and disease resistance of seeds, and at the same time providing the nutrients and regulators required for seed growth to promote seed germination and growth.

[0061] The electric cylinder 207, vibration motor 208, electric heating tube 302, first fan 303, electric rotating table 401, first drive motor 406, second fan 502, second drive motor 601 and third drive motor 702 of the lectin coating agent and coating equipment for strong gluten wheat of the present invention are purchased on the market, and technicians in the industry only need to install and operate them according to the accompanying instruction manual, without the need for creative labor by technicians in this field.

[0062] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A lectin coating agent for strong gluten wheat, It is characterized in that The invention comprises a fruiting body, a buffer, a biological fertilizer, a microbial agent and a filler. The fruiting body is a large edible fungus, and the buffer is a phosphate buffer.

2. The lectin coating agent for strong gluten wheat according to claim 1, It is characterized in that The macrofungus edible mushrooms include Agaricus bisporus.

3. The lectin coating agent for strong gluten wheat according to claim 1, It is characterized in that The preparation method of the lectin coating agent comprises the following steps: S1. Extract lectins from multiple Agaricus bisporus fruiting bodies cultivated by the research group, draw standard curves, determine the lectin content, and statistically analyze and screen out strains with high lectin content; S2, placing the Agaricus bisporus fruiting bodies with high lectin content in a food drying machine at 60° C. and drying for 24 hours to prepare a dry product; S3, crush the dry product with a grinder and filter it with a 35-mesh sieve, weigh and record the crushed Agaricus bisporus dry product after screening, mix it with 20 mM phosphate buffer at a ratio of 1:10, add it to a 50 mL centrifuge tube, shake well and let it stand and soak at 4 ° C for 12 hours; S4, centrifuge the soaking liquid in a refrigerated centrifuge at 4°C, 12000r / min for 22min, take the supernatant and filter the supernatant solids, centrifuge the filtered supernatant again at 4°C, 12000r / min for 22min, filter the supernatant, slowly add (NH4)2SO4 to the filtered solution in an ice water bath to precipitate, saturation reaches 40%, slowly stir at 4°C for 2h, centrifuge at 12000r / min for 30min, take the supernatant and discard the precipitate, take the supernatant and slowly add (NH4)2SO4 again to saturation reach 60%, slowly stir at 4°C for 4h, finally centrifuge the stirred and mixed sample at 4°C, 12000r / min for 30min, and discard the supernatant; S5, dissolve the precipitate base liquid with 20mM PBS buffer and pour it into a 35mm diameter dialysis bag for dialyzed desalination, and soak the dialysis bag in a solution of 0.2M PBS phosphate buffer diluted 1:10 with pure water, add magnetic beads and stir the solution at low temperature on a magnetic stirrer for 10h, then put the dialysate into a centrifuge tube at 4℃, 12000r / min for 30min to obtain the precipitate, and then dissolve and concentrate the precipitate with 20mM PBS buffer to obtain a crude sample solution; S6, placing the crude sample solution in a freeze dryer for freeze drying to obtain a lyophilized crude lectin product, and then dissolving the lyophilized crude product in 20 mM Tris-HCl buffer (pH=7.8) to obtain Agaricus bisporus lectin; S7. Evenly mix the Agaricus bisporus lectin with the biological fertilizer, the microbial agent and the filler to obtain the lectin coating agent.

4. A lectin coating device for strong gluten wheat, It is characterized in that The invention comprises a driving device, a collecting device, a heating device, a spraying device, a first box (101), a screen cylinder (102), blades (103), a conical cylinder (104), a baffle (105), a conveying box (106) and a ring body (107); an air intake valve is arranged on the first box (101); a take-in and put-out opening is arranged on the right side wall of the first box (101); a through hole is arranged on the left end of the first box (101); the screen cylinder (102) is rotatably installed in the first box (101) through the driving device; the opening of the screen cylinder (102) is communicated with the take-in and put-out opening of the first box (101); a plurality of blades (103) are circumferentially installed on the inner side wall of the screen cylinder (102); the conical cylinder (104) is passed through the first box (107); 01) extends into the interior of the screen cylinder (102), and the upper half of the conical cylinder (104) is provided with screen holes, the baffle (105) is installed at the right end of the conical cylinder (104), the ring body (107) is sleeved on the left side wall of the conical cylinder (104), the conveying box (106) is connected to the left end of the conical cylinder (104), the conveying box (106) is installed on the collecting device, the collecting device is used to drive the conveying box (106) to move left and right, the collecting device is used to collect the screened wheat, and the collecting device is used to remove dust from the wheat, the spraying device is communicated with the screen cylinder (102), the spraying device is used to spray the coating agent, the heating device is communicated with the first box body (101), and the heating device is used to dry the coated wheat.

5. A lectin coating device for strong gluten wheat as claimed in claim 4, It is characterized in that The collecting device comprises a power device, a dust collecting device, a second box (201), a guide platform (202), a plurality of telescopic rods (203), a plurality of springs (204), a support net (205), a frame (206), an electric cylinder (207), a vibration motor (208), a first nozzle (209) and a closed door (210); the second box (201) is slidably mounted on the guide platform (202) by the power device; the conveying box (106) is connected and arranged at the top of the second box (201); the plurality of telescopic rods (203) are all mounted at the bottom of the inner side wall of the second box (201); the plurality of springs (204) are respectively fitted on the outer side walls of the plurality of telescopic rods (203); and the frame (206) is provided with a plurality of springs. 06) is installed on the top of multiple groups of telescopic rods (203), the left end of the support net (205) is rotatably installed on the frame (206), the fixed end of the electric cylinder (207) is rotatably installed on the outer wall of the frame (206), the movable end of the electric cylinder (207) is rotatably connected to the left part of the support net (205), the vibration motor (208) is installed on the outer wall of the frame (206), the first nozzle (209) is installed on the top of the second box (201), and the first nozzle (209) is connected to the heating device, the outer wall of the second box (201) is provided with an exhaust port, and the closed door (210) is installed at the exhaust port, the dust suction device is connected to the conveying box (106), and the dust suction device is used to suck air into the conveying box (106).

6. A lectin coating device for strong gluten wheat as claimed in claim 4, It is characterized in that The heating device comprises a third box (301), an electric heating pipe (302), a first fan (303), a first hose (304) and a second hose (305); the third box (301) is arranged in communication with the lower part of the outer wall of the first box (101); the electric heating pipe (302) is installed inside the third box (301); the output end of the first fan (303) is in communication with the inside of the third box (301); the input end of the first fan (303) is in communication with the first hose (304); the input end of the first hose (304) is in communication with the inside of the second box (201); the input end of the second hose (305) is arranged in communication with the top end of the first box (101); and the output end of the second hose (305) is in communication with the first nozzle (209).

7. A lectin coating device for strong gluten wheat as claimed in claim 4, It is characterized in that The spraying device comprises an electric rotating platform (401), a support arm (402), a cover body (403), a second nozzle (404), a first gear (405), a first driving motor (406) and a rack (407); the electric rotating platform (401) is mounted on the top of the first box body (101); the support arm (402) is slidably mounted on the rotating end of the electric rotating platform (401); the cover body (403) is mounted on the bottom of the support arm (402); the second nozzle (404) is mounted on the outer wall of the cover body (403); the first gear (405) is rotatably mounted on the inner wall of the rotating end of the electric rotating platform (401); the first driving motor (406) is mounted on the outer wall of the rotating end of the electric rotating platform (401); the output end of the first driving motor (406) is connected to the first gear (405); the rack (407) is arranged on the outer wall of the support arm (402); and the rack (407) is meshed with the first gear (405).

8. The lectin coating device for strong gluten wheat according to claim 5, It is characterized in that The dust collecting device comprises a collection box (501), a second fan (502) and a filter (503); the collection box (501) is arranged on the outer side wall of the second drive motor (601) in communication with the collection box (501); the second fan (502) is arranged on the outer side wall of the collection box (501) in communication with the collection box (501); and the filter (503) is arranged on the input end of the second fan (502).

9. The lectin coating device for strong gluten wheat according to claim 4, It is characterized in that The driving device comprises a second driving motor (601), a second gear (602) and a gear ring (603); the second driving motor (601) is mounted on the outer wall of the first box body (101); the second gear (602) is mounted on the output end of the second driving motor (601); the gear ring (603) is mounted on the outer wall of the screen cylinder (102); and the gear ring (603) is meshed with the second gear (602).

10. The lectin coating device for strong gluten wheat according to claim 5, It is characterized in that The power device comprises a lead screw (701) and a third drive motor (702); the lead screw (701) is rotatably mounted on the inner wall of the guide platform (202); the bottom of the second box body (201) is screwed on the lead screw (701); the third drive motor (702) is mounted on the outer wall of the guide platform (202); and the output end of the third drive motor (702) is connected to the lead screw (701).

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