Application of vanillyl butyl ether in skin external preparation, vanillyl butyl ether cyclodextrin inclusion compound and preparation method of fiber mask

Abstract

The invention discloses an application of vanillyl butyl ether in an external preparation for skin, a vanillyl butyl ether cyclodextrin inclusion compound and a preparation method of a fiber mask. The vanillyl butyl ether is used as an antioxidant active component and a heating component in the external preparation for skin, and the addition amount of the vanillyl butyl ether in the external preparation for skin is 0.01-10wt%; the invention also provides a preparation method of the vanillyl butyl ether cyclodextrin inclusion compound. The preparation method comprises the following steps: (1) dissolving vanillyl butyl ether in ethanol to prepare a vanillyl butyl ether alcohol solution; (2) dissolving the cyclodextrin in water, and stirring until the cyclodextrin is completely dissolved to prepare a cyclodextrin aqueous solution; and (3) dropwise adding the vanillyl butyl ether alcohol solution prepared in the step (1) into the cyclodextrin aqueous solution prepared in the step (2), stirring for clathration, removing the solvent, and drying. The vanillyl butyl ether is coated, the solubility of the vanillyl butyl ether in water is improved, and the nanofiber thermal inductance mask is prepared by an electrostatic spinning method and has ideal effects of oxidation resistance, heating and the like.

Description

Technical Field

[0001] The present application belongs to the field of cosmetic technology, and in particular to an application of vanillyl butyl ether in a skin external preparation, a vanillyl butyl ether cyclodextrin inclusion compound, and a fiber mask preparation method. Background Art

[0002] The human body produces free radicals in the normal metabolic process, including breathing, external pollution, radiation exposure and other factors. The accumulation of these free radicals will cause oxidative stress and various pathological reactions. The proper use of antioxidants can effectively inhibit the oxidation reaction of free radicals and alleviate skin aging and other problems. Therefore, among the product functions, antioxidant is listed as one of the main research and development directions by cosmetics companies, and it is also one of the most important functional demands of the market.

[0003] Vanillyl butyl ether can be extracted from rosemary and sage. It has been proven that it produces a mild and lasting warm sensation after being applied to the skin, which can promote capillary blood circulation and accelerate the absorption of active ingredients. It is often added to cosmetics and toiletries as a warming agent. However, there are no reports on other properties of vanillyl butyl ether. In addition, vanillyl butyl ether has poor solubility in water, which limits its wide application. It is currently only used in oil phase systems.

[0004] As a commonly used skin care product, facial masks have the functions of rapid hydration and skin care, and have become an indispensable part of people's daily life. However, facial masks on the market are usually water-based sheet masks, which contain a variety of inactive ingredients, such as preservatives and thickeners, which can easily cause skin allergies, accelerate aging and other skin problems. Therefore, people are more inclined to accept novel, green and safe facial masks to reduce damage to the skin, but there are few reports on heating nanofiber masks.

[0005] Therefore, there is an urgent need to develop the potential properties of vanillyl butyl ether and improve the water solubility of vanillyl butyl ether to expand its application in skin care products or other daily chemical products. Summary of the invention

[0006] The technical problem to be solved by this application is to develop the potential properties of vanillyl butyl ether, overcome the defects of poor water solubility of vanillyl butyl ether, lack of modification methods of vanillyl butyl ether, and lack of exploration of the activity of vanillyl butyl ether cyclodextrin inclusion complex after preparing facial mask by electrospinning electrospinning method, and provide an application of vanillyl butyl ether in skin topical preparations, vanillyl butyl ether cyclodextrin inclusion complex and fiber mask preparation method. This application has found through research that vanillyl butyl ether has antioxidant activity. By utilizing the special structure of cyclodextrin and coating vanillyl butyl ether through encapsulation technology, the high solubility of vanillyl butyl ether in water is achieved, and its application level is improved. A nanofiber mask is successfully prepared by electrospinning, achieving ideal antioxidant efficacy and mild thermal effect.

[0007] This application adopts the following technical solutions to solve the above technical problems:

[0008] The present application provides a use of vanillyl butyl ether in a skin topical preparation, wherein vanillyl butyl ether is used as an antioxidant active ingredient in the skin topical preparation;

[0009] Wherein, the vanillyl butyl ether is added in an amount of 0.01 to 10 wt % in the skin external preparation.

[0010] In some embodiments, the vanillyl butyl ether is included in cyclodextrin to obtain a vanillyl butyl ether cyclodextrin inclusion complex, which is used in the skin topical preparation.

[0011] In some embodiments, the vanillyl butyl ether is added in an amount of 0.05-6 wt %, for example 1 wt % in the skin external preparation.

[0012] In some embodiments, the method for preparing the vanillyl butyl ether cyclodextrin inclusion compound specifically comprises the following steps:

[0013] (1) dissolving vanillyl butyl ether in ethanol to prepare a vanillyl butyl ether alcohol solution;

[0014] (2) dissolving the cyclodextrin in water and stirring until it is completely dissolved to prepare a cyclodextrin aqueous solution;

[0015] (3) dropping the vanillyl butyl ether alcohol solution prepared in step (1) into the cyclodextrin aqueous solution prepared in step (2), stirring for inclusion, removing the solvent, and drying.

[0016] Wherein, in step (1), the concentration of vanillyl butyl ether in the vanillyl butyl ether alcohol solution is 0.008 to 0.1 g / mL, preferably 0.03 to 0.05 g / mL.

[0017] In step (1), the ethanol is anhydrous ethanol.

[0018] In step (1), the dissolution is carried out under stirring conditions, and the stirring is conventional in the art, and is generally carried out under a magnetic stirrer.

[0019] Wherein, when the magnetic stirrer is used for stirring, the stirring speed is 100 to 800 rpm, preferably 300 to 400 rpm.

[0020] When a magnetic stirrer is used for stirring, the stirring time is 60 to 120 minutes, preferably 90 minutes.

[0021] In step (1), the dissolving is carried out at room temperature.

[0022] In step (2), the concentration of cyclodextrin in the cyclodextrin aqueous solution is 0.007-0.5 g / mL, preferably 0.045-0.055 g / mL.

[0023] In step (2), the stirring is conventionally performed in the art, and is generally performed under magnetic stirring.

[0024] Wherein, when a magnetic stirrer is used for stirring, the rotation speed is 100 to 800 rpm, preferably 300 to 400 rpm.

[0025] When a magnetic stirrer is used for stirring, the stirring time is 60 to 120 minutes, preferably 90 minutes.

[0026] In step (2), the cyclodextrin includes one of hydroxypropyl-β-cyclodextrin, sulfobutyl-β-cyclodextrin, and methyl-β-cyclodextrin, preferably hydroxypropyl-β-cyclodextrin.

[0027] In step (3), after the vanillyl butyl ether alcohol solution is mixed with the cyclodextrin aqueous solution, the molar ratio of the vanillyl butyl ether to the cyclodextrin is 1:(1-2).

[0028] In step (3), the dripping rate is 1-5 mL / min, preferably 3 mL / min.

[0029] In step (3), the stirring is conventionally performed in the art, and is generally performed under magnetic stirring.

[0030] Wherein, when a magnetic stirrer is used for stirring, the stirring speed is 100 to 800 rpm, preferably 300 to 400 rpm.

[0031] When a magnetic stirrer is used for stirring, the stirring time is 6 to 24 hours, preferably 12 hours.

[0032] In step (3), the inclusion method is: including the vanillyl butyl ether in the cavity structure inside the cyclodextrin to carry out an inclusion reaction.

[0033] In step (3), the method for removing the solvent comprises evaporation under reduced pressure.

[0034] When the reduced pressure evaporation method is adopted, it can be carried out in a rotary evaporator according to the conventional method in the art.

[0035] Wherein, the temperature of the reduced pressure evaporation may be 35-45°C.

[0036] The time of the reduced pressure evaporation may be 20 to 45 minutes.

[0037] In some embodiments, in step (3), the drying is conventional in the art, generally freeze-drying.

[0038] When the freeze drying is used for the drying, the process also includes pre-freezing the sample. Preferably, the pre-freezing temperature is -30 to -10°C, preferably -20°C.

[0039] When the pre-freezing operation is adopted, the pre-freezing time is 2 to 24 hours, preferably 12 hours.

[0040] When freeze drying is used for the drying, the freeze drying temperature is -90 to -70°C, preferably -80°C.

[0041] When freeze drying is used for the drying, the freeze drying time is 24 to 72 hours, preferably 48 hours.

[0042] In some embodiments, the vanillyl butyl ether is used as an antioxidant active ingredient in the skin topical preparation having an ABTS free radical scavenging effect and / or a DPPH free radical scavenging effect.

[0043] The vanillyl butyl ether cyclodextrin inclusion compound is used as an antioxidant active ingredient in the skin external preparation having an ABTS free radical scavenging effect and / or a DPPH free radical scavenging effect.

[0044] Among them, the vanillyl butyl ether or the vanillyl butyl ether cyclodextrin inclusion compound is a component with a fever effect in the skin external preparation, and the fever effect component has a fever effect in 1 to 15 minutes and lasts for 15 to 30 minutes.

[0045] Wherein, the skin external preparation includes a facial mask, lotion or cream, preferably a facial mask, such as a nanofiber facial mask.

[0046] In some embodiments, the nanofiber mask is prepared by an electrospinning method, and the electrospinning method specifically comprises the following steps:

[0047] I. Compounding the vanillyl butyl ether cyclodextrin inclusion compound and the functional ingredients in a polymer spinning aid solution, stirring, and ultrasonically treating to obtain a spinning solution;

[0048] II. Fix the spinning solution and spray it into the receiving device.

[0049] In some embodiments, in step (I), the mass concentration of the vanillyl butyl ether cyclodextrin inclusion complex is 0.1-20 wt %, preferably 2-5 wt %.

[0050] In step (I), the polymer spinning aid solution may generally include at least one of polyvinyl alcohol, polyethylene glycol, polyvinyl pyrrolidone, pullulan, pectin, gelatin, gum arabic and sodium alginate.

[0051] In step (I), the concentration of the polymer spinning aid solution is 5 to 50 wt %, preferably 8 to 20 wt %.

[0052] In step (I), the functional ingredients may also include active ingredients conventionally used in the art, generally including at least one of moisturizing active ingredients, whitening active ingredients, anti-inflammatory active ingredients, anti-allergic active ingredients and antioxidant active ingredients.

[0053] Wherein, the moisturizing active ingredient includes sodium hyaluronate. When the sodium hyaluronate is added, the concentration of the sodium hyaluronate is 0.5-10wt%, preferably 1-4wt%.

[0054] In some embodiments, in step (I), the stirring is performed until the material is completely dissolved. The stirring time is 2 to 24 hours, preferably 12 hours.

[0055] In some embodiments, in step (I), the ultrasonic treatment is ultrasonic degassing treatment.

[0056] Wherein, when the ultrasonic degassing treatment is adopted, the ultrasonic time is 10 to 20 minutes, preferably 15 minutes.

[0057] Wherein, when the ultrasonic degassing treatment is adopted, the frequency of the ultrasound is 150-300W, preferably 200-250W.

[0058] In some embodiments, in step (II), the fixation is conventional in the art, and is generally performed on an electrospinning device.

[0059] Wherein, when the fixing is performed on the electrospinning device, the fixing voltage is 9 to 20 KV, preferably 12 to 16 KV, and more preferably 14 to 15 KV.

[0060] In some embodiments, in step (II), the spraying is conventional injection spraying in the art, generally using a syringe to absorb the spinning solution and spraying it under the non-woven mask.

[0061] Preferably, the spraying flow rate is 0.2-0.6 mL / h, more preferably 0.3-0.4 mL / h.

[0062] Preferably, the spraying time is 1 to 20 hours, more preferably 4 to 8 hours.

[0063] Preferably, the receiving distance of the spraying is 5 to 30 cm, more preferably 15 to 20 cm, for example 15 cm.

[0064] The present application also provides a fiber mask comprising a vanillyl butyl ether cyclodextrin inclusion complex, which is prepared by the electrospinning method as described above.

[0065] On the basis of conforming to the common sense in the art, the above-mentioned preferred conditions can be arbitrarily combined to obtain the preferred embodiments of the present application.

[0066] The reagents and raw materials used in this application are commercially available.

[0067] The positive effects of this application are:

[0068] After research, the present application found that vanillyl butyl ether has potential antioxidant activity due to the presence of a phenolic hydroxyl group in its structure. At the same time, the present application utilizes the special structure of cyclodextrin to coat vanillyl butyl ether through the preparation method of the present application, achieving high solubility in water, and applying it to the base liquid. It can also be compounded with other functional ingredients and film-forming agents to exert a synergistic effect. At the same time, the present application successfully prepared a nanofiber mask using high-voltage electrospinning. Through free radical scavenging experiments and human thermal sensation experiments, it was confirmed that it has ideal antioxidant, heat-generating and other effects, and does not require additives such as preservatives and stabilizers. It is highly safe to use and comfortable to experience. BRIEF DESCRIPTION OF THE DRAWINGS

[0069] The present application may be better understood by referring to the following description in conjunction with the accompanying drawings. The accompanying drawings, together with the following detailed description, are included in and form a part of this specification and are used to further illustrate the preferred embodiments of the present application and explain the principles and advantages of the present application. Among them:

[0070] Figure 1 This is a diagram showing the scavenging effect of vanillyl butyl ether ethanol solutions of different concentrations on DPPH free radicals;

[0071] Figure 2 This is a scanning electron microscope image of the inclusion complex of hydroxypropyl-β-cyclodextrin and vanillyl butyl ether cyclodextrin obtained in Example 1 of the present application;

[0072] Figure 3 This is a thermogravimetric analysis curve of the vanillyl butyl ether and the vanillyl butyl ether cyclodextrin inclusion complex obtained in Example 1 of the present application;

[0073] Figure 4 This is a scanning electron microscope image of the nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate obtained in Example 1 of the present application;

[0074] Figure 5The sustained release graph of vanillyl butyl ether of the nanofiber mask of the vanillyl butyl ether cyclodextrin inclusion compound and the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion compound / sodium hyaluronate obtained in Example 1 of the present application;

[0075] Figure 6 This is a comparison chart of the scavenging effects of the vanillyl butyl ether cyclodextrin inclusion complex on ABTS free radicals at different concentrations obtained in Examples 1 to 3 of the present application and Comparative Examples 1 to 3;

[0076] Figure 7 This is a comparison chart of the scavenging effects of the vanillyl butyl ether cyclodextrin inclusion complex on DPPH free radicals at different concentrations obtained in Examples 1 to 3 of the present application and Comparative Examples 1 to 3;

[0077] Figure 8 This is a comparison chart of the scavenging effects of the nanofiber mask of vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate obtained in Examples 1 to 3 of the present application and Comparative Examples 1 to 3 on ABTS free radicals. DETAILED DESCRIPTION

[0078] The present application is further described below by way of examples, but the present application is not limited to the scope of the examples. The experimental methods without specific conditions in the following examples are selected according to conventional methods and conditions, or according to the product instructions.

[0079] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0080] The vanillyl butyl ether in the following examples and comparative examples was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0081] The ethanol in the following examples and comparative examples was purchased from Shanghai Titan Technology Co., Ltd.

[0082] The hydroxypropyl-β-cyclodextrin in the following examples and comparative examples was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0083] The β-cyclodextrin in the following examples and comparative examples was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0084] The sodium hyaluronate in the following examples and comparative examples was purchased from Bloomage Biotech Co., Ltd.

[0085] The pullulan in the following examples and comparative examples was purchased from Zhongke Hongji Biotechnology Co., Ltd.

[0086] Example 1

[0087] A method for preparing a vanillyl butyl ether cyclodextrin inclusion compound comprises the following steps:

[0088] (1) Weigh 0.5 g of vanillyl butyl ether and dissolve it in 10 mL of anhydrous ethanol. Stir the mixture under magnetic force until it is completely dissolved at room temperature to prepare a vanillyl butyl ether alcohol solution with a concentration of 0.05 g / mL.

[0089] (2) Weigh 3.67 g of hydroxypropyl-β-cyclodextrin and dissolve it in 70 mL of deionized water. Stir it magnetically until it is completely dissolved to prepare a hydroxypropyl-β-cyclodextrin aqueous solution with a concentration of 0.0524 g / mL;

[0090] (3) 10 mL of vanillyl butyl ether ethanol solution was dripped into 70 mL of cyclodextrin aqueous solution at a rate of 3 mL / min, and magnetic stirring was performed at a rotation speed of 300 rpm for 12 h to carry out inclusion reaction to obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and evaporated under reduced pressure to remove the solvent. The heating temperature of the rotary evaporator was 40°C, and the reduced pressure evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20°C for 12 h, and then freeze-dried at -80°C for 48 h to obtain a white loose inclusion complex solid, i.e., vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.

[0091] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate comprises the following steps:

[0092] (I) 5 g of pullulan was added to 45 g of water, stirred at room temperature for 4 h until the pullulan was completely dissolved, then the stirring was stopped, and ultrasonic degassing was performed for 15 min to obtain a pullulan solution (wherein the concentration of pullulan was 10 wt %;). 0.5 g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion compound prepared above and 0.5 g of sodium hyaluronate were weighed and added to the pullulan solution, stirred for 4 h until both were completely dissolved, then the stirring was stopped, and then ultrasonic treatment was performed for 15 min for degassing to obtain a spinning solution.

[0093] (II) The spinning solution in step (I) was absorbed by a 5 mL syringe and fixed on an electrospinning device. The fixed voltage was 15 kV, the receiving distance was 15 cm, the liquid pushing flow rate was 0.4 mL / h, and the receiving screen was a non-woven mask cloth. The spraying was performed for 5 hours. After the spraying was completed, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate was obtained.

[0094] Example 2

[0095] A method for preparing a vanillyl butyl ether cyclodextrin inclusion compound comprises the following steps:

[0096] (1) Weigh 0.5 g of vanillyl butyl ether and dissolve it in 10 mL of anhydrous ethanol. Stir the solution under magnetic force until it is completely dissolved at room temperature to prepare a vanillyl butyl ether ethanol solution with a concentration of 0.05 g / mL;

[0097] (2) Weigh 7.33 g of hydroxypropyl-β-cyclodextrin and dissolve it in 140 mL of deionized water. Stir magnetically at room temperature until it is completely dissolved to prepare a hydroxypropyl-β-cyclodextrin aqueous solution with a concentration of 0.0524 g / mL;

[0098] (3) 10 mL of vanillyl butyl ether ethanol solution was dripped into 140 mL of cyclodextrin aqueous solution at a rate of 3 mL / min, and magnetic stirring was performed at a rotation speed of 300 rpm for 12 h to carry out inclusion reaction to obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and evaporated under reduced pressure to remove the solvent. The heating temperature of the rotary evaporator was 40°C, and the reduced pressure evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20°C for 12 h, and then freeze-dried at -80°C for 48 h to obtain a white loose inclusion complex solid, i.e., vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.

[0099] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate comprises the following steps:

[0100] (I) 5 g of pullulan was added to 45 g of water, stirred at room temperature for 4 h until the pullulan was completely dissolved, then the stirring was stopped, and ultrasonic degassing was performed for 15 min to obtain a pullulan solution (wherein the concentration of pullulan was 10 wt %;). 0.5 g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion compound prepared above and 0.5 g of sodium hyaluronate were weighed and added to the pullulan solution, stirred for 4 h until both were completely dissolved, then the stirring was stopped, and then ultrasonic treatment was performed for 15 min for degassing to obtain a spinning solution.

[0101] (II) The spinning solution in step (I) was absorbed by a 5 mL syringe and fixed on an electrospinning device. The fixed voltage was 15 kV, the receiving distance was 15 cm, the liquid pushing flow rate was 0.4 mL / h, and the receiving screen was a non-woven mask cloth. The spraying was performed for 5 hours. After the spraying was completed, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate was obtained.

[0102] Example 3

[0103] A method for preparing a vanillyl butyl ether cyclodextrin inclusion compound comprises the following steps:

[0104] (1) Weigh 0.5 g of vanillyl butyl ether and dissolve it in 10 mL of anhydrous ethanol. Stir the mixture under magnetic force until it is completely dissolved at room temperature to prepare a vanillyl butyl ether alcohol solution with a concentration of 0.05 g / mL.

[0105] (2) Weigh 3.67 g of hydroxypropyl-β-cyclodextrin and dissolve it in 70 mL of deionized water. Stir it with a magnetic stirrer at room temperature until it is completely dissolved to prepare a hydroxypropyl-β-cyclodextrin aqueous solution with a concentration of 0.0524 g / mL;

[0106] (3) 50 mL of vanillyl butyl ether ethanol solution was dripped into 100 mL of cyclodextrin aqueous solution at a rate of 3 mL / min, and magnetic stirring was performed at a rotation speed of 300 rpm for 8 h to carry out inclusion reaction to obtain vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and evaporated under reduced pressure to remove the solvent. The heating temperature of the rotary evaporator was 40°C, and the reduced pressure evaporation time was 40 min. The concentrated inclusion complex solution was pre-frozen at -20°C for 12 h, and then freeze-dried at -80°C for 48 h to obtain a white loose inclusion complex solid, i.e., vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.

[0107] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate comprises the following steps:

[0108] (I) 5 g of pullulan was added to 45 g of water, stirred at room temperature for 4 h until the pullulan was completely dissolved, then the stirring was stopped, and ultrasonic degassing was performed for 15 min to obtain a pullulan solution (wherein the concentration of pullulan was 10 wt %;). 0.5 g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion compound prepared above and 0.5 g of sodium hyaluronate were weighed and added to the pullulan solution, stirred for 4 h until both were completely dissolved, then the stirring was stopped, and then ultrasonic treatment was performed for 15 min for degassing to obtain a spinning solution.

[0109] (II) The spinning solution in step (I) was absorbed by a 5 mL syringe and fixed on an electrospinning device. The fixed voltage was 15 kV, the receiving distance was 15 cm, the liquid pushing flow rate was 0.4 mL / h, and the receiving screen was a non-woven mask cloth. The spraying was performed for 5 hours. After the spraying was completed, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate was obtained.

[0110] Comparative Example 1

[0111] Compared with Example 1, the only difference is that the reagent used is β-cyclodextrin, and the specific operation is as follows:

[0112] (1) Weigh 0.5 g of vanillyl butyl ether and dissolve it in 10 mL of anhydrous ethanol. Stir the mixture under magnetic force until it is completely dissolved at room temperature to prepare a vanillyl butyl ether alcohol solution with a concentration of 0.05 g / mL.

[0113] (2) Weigh 2.7 g of β-cyclodextrin and dissolve it in 70 mL of deionized water. Stir magnetically until it is completely dissolved at room temperature to prepare a β-cyclodextrin aqueous solution with a concentration of 0.0386 g / mL.

[0114] (3) 10 mL of vanillyl butyl ether ethanol solution was dripped into 70 mL of β-cyclodextrin aqueous solution at a rate of 3 mL / min, and magnetic stirring was performed at a rotation speed of 500 rpm for 12 h to carry out inclusion reaction to obtain a vanillyl butyl ether-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and evaporated under reduced pressure to remove the solvent. The heating temperature of the rotary evaporator was 40°C, and the reduced pressure evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20°C for 12 h, and then freeze-dried at -80°C for 48 h to obtain a white loose inclusion complex solid, i.e., vanillyl butyl ether-β-cyclodextrin inclusion complex.

[0115] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-β-cyclodextrin inclusion complex / sodium hyaluronate comprises the following steps:

[0116] (I) 5 g of pullulan was added to 45 g of water, stirred at room temperature for 4 h until the pullulan was completely dissolved, then the stirring was stopped, and ultrasonic degassing was performed for 15 min to obtain a pullulan solution (wherein the concentration of pullulan was 10 wt %). 0.5 g of vanillyl butyl ether-β-cyclodextrin inclusion complex and 0.5 g of sodium hyaluronate were weighed and added to the pullulan solution, stirred for 4 h until both were completely dissolved, then the stirring was stopped, and then ultrasonic degassing was performed for 15 min to obtain a spinning solution.

[0117] (II) The spinning solution in step (I) was absorbed by a 5 mL syringe and fixed on an electrospinning device. The fixed voltage was 15 kV, the receiving distance was 15 cm, the liquid push flow rate was 0.4 mL / h, and the receiving screen was a non-woven mask cloth. The spraying was performed for 5 hours. After the spraying was completed, a nanofiber mask containing vanillyl butyl ether-β-cyclodextrin inclusion complex / sodium hyaluronate was obtained.

[0118] Comparative Example 2

[0119] Compared with Example 1, the only difference is that the molar ratio of vanillyl butyl ether to hydroxypropyl-β-cyclodextrin is 1:4. The specific operation is as follows:

[0120] (1) Weigh 0.5 g of vanillyl butyl ether and dissolve it in 10 mL of anhydrous ethanol. Stir the mixture under magnetic force until it is completely dissolved at room temperature to prepare a vanillyl butyl ether alcohol solution with a concentration of 0.05 g / mL.

[0121] (2) Weigh 14.68 g of hydroxypropyl-β-cyclodextrin and dissolve it in 280 mL of deionized water. Stir magnetically at room temperature until it is completely dissolved to prepare a 0.0524 g / mL hydroxypropyl-β-cyclodextrin aqueous solution.

[0122] (3) 10 mL of vanillyl butyl ether ethanol solution was dripped into 280 mL of cyclodextrin aqueous solution at a rate of 3 mL / min, and magnetic stirring was performed at a rotation speed of 300 rpm for 12 h to carry out inclusion reaction to obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and evaporated under reduced pressure to remove the solvent. The heating temperature of the rotary evaporator was 40°C, and the reduced pressure evaporation time was 30 min. The concentrated inclusion complex solution was pre-frozen at -20°C for 12 h, and then freeze-dried at -80°C for 48 h to obtain a white loose inclusion complex solid, i.e., vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.

[0123] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate comprises the following steps:

[0124] (I) 5 g of pullulan was added to 45 g of water, stirred at room temperature for 4 h until the pullulan was completely dissolved, then the stirring was stopped, and ultrasonic degassing was performed for 15 min to obtain a pullulan solution (wherein the concentration of pullulan was 10 wt %;). 0.5 g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion compound prepared above and 0.5 g of sodium hyaluronate were weighed and added to the pullulan solution, stirred for 4 h until both were completely dissolved, then the stirring was stopped, and then ultrasonic treatment was performed for 15 min for degassing to obtain a spinning solution.

[0125] (II) The spinning solution in step (I) was absorbed by a 5 mL syringe and fixed on an electrospinning device. The fixed voltage was 15 kV, the receiving distance was 15 cm, the liquid pushing flow rate was 0.4 mL / h, and the receiving screen was a non-woven mask cloth. The spraying was performed for 5 hours. After the spraying was completed, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate was obtained.

[0126] Comparative Example 3

[0127] Compared with Example 1, the difference is that the operation in step (3) is different. The specific operation is as follows:

[0128] (1) Weigh 0.5 g of vanillyl butyl ether and dissolve it in 10 mL of anhydrous ethanol. Stir the mixture under magnetic force until it is completely dissolved at room temperature to prepare a vanillyl butyl ether alcohol solution with a concentration of 0.05 g / mL.

[0129] (2) Weigh 3.67 g of hydroxypropyl-β-cyclodextrin and dissolve it in 70 mL of deionized water. Stir it with a magnetic stirrer at room temperature until it is completely dissolved to prepare a hydroxypropyl-β-cyclodextrin aqueous solution with a concentration of 0.0524 g / mL;

[0130] (3) 10 mL of vanillyl butyl ether ethanol solution was dripped into 70 mL of cyclodextrin aqueous solution at a rate of 3 mL / min, and magnetic stirring was performed at a rotation speed of 300 rpm for 1 hour to carry out inclusion reaction to obtain a vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex solution. The stirred inclusion complex solution was placed in a rotary evaporator and evaporated under reduced pressure to remove the solvent. The heating temperature of the rotary evaporator was 40°C, and the reduced pressure evaporation time was 30 minutes. The concentrated inclusion complex solution was pre-frozen at -20°C for 12 hours, and then freeze-dried at -80°C for 48 hours to obtain a white loose inclusion complex solid, i.e., vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex.

[0131] A method for preparing a nanofiber facial mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate comprises the following steps:

[0132] (I) 5 g of pullulan was added to 45 g of water, stirred at room temperature for 4 h until the pullulan was completely dissolved, then the stirring was stopped, and ultrasonic degassing was performed for 15 min to obtain a pullulan solution (wherein the concentration of pullulan was 10 wt %;). 0.5 g of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion compound prepared above and 0.5 g of sodium hyaluronate were weighed and added to the pullulan solution, stirred for 4 h until both were completely dissolved, then the stirring was stopped, and then ultrasonic treatment was performed for 15 min for degassing to obtain a spinning solution.

[0133] (II) The spinning solution in step (I) was absorbed by a 5 mL syringe and fixed on an electrospinning device. The fixed voltage was 15 kV, the receiving distance was 15 cm, the liquid pushing flow rate was 0.4 mL / h, and the receiving screen was a non-woven mask cloth. The spraying was performed for 5 hours. After the spraying was completed, a nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate was obtained.

[0134] Effect Example 1

[0135] Determination of DPPH free radical scavenging effect. DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) is a stable organic free radical with a maximum absorption peak of 517nm in the visible light region. The higher the DPPH free radical scavenging rate, the more ideal the antioxidant effect. Weigh 20mg of DPPH in a 250mL volumetric flask, dilute with anhydrous ethanol, and prepare a 0.2mmol / L DPPH ethanol solution. Dissolve vanillyl butyl ether in ethanol solution, and prepare solutions with concentrations of 0.001mg / mL, 0.005mg / mL, 0.01mg / mL, 0.05mg / mL, 0.1mg / mL, 0.5mg / mL, 1mg / mL, 5mg / mL and 10mg / mL as the test solution. Mix 100μL of the test solution with an equal amount of DPPH ethanol solution as a sample group, shake slightly, incubate in the dark for 30min, and measure the absorbance at 517nm. At the same time, the DPPH solution and an equal volume of anhydrous ethanol mixed solution were measured as a blank group, and the test solution and an equal volume of anhydrous ethanol mixed solution were measured as a solvent base value group.

[0136] DPPH free radical scavenging rate (%) = [1-(A 样品组 -A 样品底值 ) / (A 空白组 -A 溶剂底值 )]×100%

[0137] Table 1

[0138]

[0139]

[0140] Figure 1 Table 1 shows that vanillyl butyl ether has an excellent DPPH free radical scavenging rate, which shows that vanillyl butyl ether has a good antioxidant effect, and in the range of 0.001 mg / mL to 10 mg / mL, the DPPH free radical scavenging rate is concentration-dependent.

[0141] Effect Example 2

[0142] The microstructure of the vanillyl butyl ether cyclodextrin inclusion complex prepared in Example 1 was observed using a ZEISS GeminiSEM 300 scanning electron microscope at a test voltage of 5 kV. Figure 2 .

[0143] Depend on Figure 2It can be seen that hydroxypropyl-β-cyclodextrin is in the form of a spherical cavity. In the scanning electron microscope of the vanillyl butyl ether cyclodextrin inclusion complex prepared in Example 1, the unique spherical cavity structure of hydroxypropyl-β-cyclodextrin disappears, and an irregular block structure can be clearly observed, indicating that vanillyl butyl ether and hydroxypropyl-β-cyclodextrin interact with each other, causing the cyclodextrin molecular morphology to change. Thermogravimetric analysis of vanillyl butyl ether and vanillyl butyl ether cyclodextrin inclusion complex is shown in Figure 3 After vanillyl butyl ether was included in cyclodextrin, the thermal stability of the inclusion complex was significantly better than that of vanillyl butyl ether.

[0144] Effect Example 3

[0145] The microstructure of the nanofiber membrane prepared in Example 1 was observed using a ZEISS GeminiSEM 300 scanning electron microscope at a test voltage of 5 kV. Figure 4 .

[0146] Depend on Figure 4 It can be seen that the average diameter of the electrospun mask is 194 nm, and the fibers are uniform, smooth, and free of beads, indicating that the preparation method of the electrospinning mask of the present application can achieve uniform mixing of vanillyl butyl ether cyclodextrin inclusion complex, sodium hyaluronate, and pullulan.

[0147] Effect Example 4

[0148] The release rate of vanillyl butyl ether of the vanillyl butyl ether cyclodextrin inclusion compound and the nanofiber mask prepared in Example 1 was tested, and the results are shown in Tables 2 and Figure 5 .

[0149] The test method includes the following steps: 100 mg of the sample to be tested is dissolved in a conical flask containing 20 ml of water, and the solution is placed in a shaker and stirred at a speed of 100 rpm. 4 mL of the release solution is taken at regular intervals, the absorbance of the solution at 280 nm is measured, and the solution is promptly poured back into the original conical flask.

[0150] Vanillyl butyl ether release rate = actual concentration of vanillyl butyl ether in the solution / theoretical concentration of vanillyl butyl ether × 100%

[0151] Table 2

[0152]

[0153] From Table 2 and Figure 5 It shows that vanillyl butyl ether has a good release effect in both the inclusion complex and the nanofiber mask of Example 1, with a rapid release in the first 10 minutes and a slow release thereafter. After 30 minutes, the release of vanillyl butyl ether from the inclusion complex can reach more than 80%, and the release of vanillyl butyl ether from the nanofiber mask can reach more than 60%.

[0154] Effect Example 5

[0155] The antioxidant capacity of the substance is evaluated by detecting the scavenging ability of ABTS free radicals. The higher the scavenging rate of ABTS free radicals, the more ideal the antioxidant effect. The ABTS working solution is prepared by incubating equal volumes of ABTS solution (7.4mmol / L) and K2S2O8 solution (2.6mmol / L) in the dark for 12h. Before the determination, the ABTS working solution is diluted with deionized water so that its absorbance range at 734nm is between 0.68-0.72. The vanillyl butyl ether cyclodextrin inclusion complex prepared in the above Examples 1 to 3 and Comparative Examples 1 to 3 is dissolved in deionized water and configured into different concentrations of the test solution. The concentrations of the vanillyl butyl ether cyclodextrin inclusion complex in the test solution are 1mg / mL, 2mg / mL, 5mg / mL and 10mg / mL respectively. It is worth noting that when the concentration of the vanillyl butyl ether-β-cyclodextrin inclusion complex prepared in Comparative Example 1 is 10 mg / mL or above, the solution becomes white and turbid, indicating that the water solubility of the vanillyl butyl ether-β-cyclodextrin inclusion complex is still low, so only the 0.5 mg / mL, 1 mg / mL and 5 mg / mL solutions thereof are subjected to free radical scavenging experiments. 50 μL of the inclusion complex solution was mixed with 150 μL of ABTS diluent as a sample group, shaken and evenly mixed, and the absorbance of the mixed solution at a wavelength of 734 nm was measured after 6 minutes. The inclusion complex solution was replaced with deionized water as a blank group, and 50 μL of the inclusion complex solution was mixed with 150 μL of deionized water as a solvent base group. The results are shown in Tables 3 and Figure 6 .

[0156] ABTS free radical scavenging rate (%) = [1-(A 样品组 -A 样品底值 ) / (A 空白组 -A 溶剂底值 )]×100%.

[0157] Table 3

[0158]

[0159] Table 3 and Figure 6 It shows that the vanillyl butyl ether inclusion complex prepared in Examples 1 to 3 has a better ABTS free radical scavenging effect than the vanillyl butyl ether inclusion complex prepared in Comparative Examples 1 to 3.

[0160] Effect Example 6

[0161] The vanillyl butyl ether cyclodextrin inclusion complex prepared in the above Examples 1 to 3 and Comparative Examples 1 to 3 was dissolved in deionized water and prepared into test solutions of different concentrations, wherein the concentrations of the inclusion complex in the test solution were 1 mg / mL, 2 mg / mL, 5 mg / mL and 10 mg / mL, respectively. 100 μL of the inclusion complex solution was mixed with an equal amount of DPPH ethanol solution as a sample group, and after slight shaking, the absorbance was measured at 517 nm after incubation in the dark for 30 min. At the same time, the DPPH solution and an equal volume of deionized water were measured as a blank group, and the inclusion complex solution and an equal volume of anhydrous ethanol mixed solution were measured as a solvent bottom value group.

[0162] DPPH free radical scavenging rate (%) = [1-(A 样品组 -A 样品底值 ) / (A 空白组 -A 溶剂底值 )]×100%

[0163] The DPPH radical scavenging rate of the vanillyl butyl ether cyclodextrin inclusion complexes prepared in Examples 1 to 3 and Comparative Examples 1 to 3 was tested. The results are shown in Tables 4 and Figure 7 .

[0164] Table 4

[0165]

[0166] From Table 4 and Figure 7 It can be seen that at the same concentration, the antioxidative effect of the vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex prepared in Examples 1 to 3 is better than that of Comparative Examples 1 to 3, and shows an increasing trend with the increase in the concentration of the vanillyl butyl ether cyclodextrin inclusion complex. Moreover, when the concentration of the vanillyl butyl ether cyclodextrin inclusion complex is increased to 10 mg / mL, the vanillyl butyl ether cyclodextrin inclusion complex in Comparative Example 1 cannot be completely dissolved, so it cannot be measured.

[0167] Effect Example 7

[0168] The ABTS free radical scavenging ability of the nanofiber mask containing vanillyl butyl ether-hydroxypropyl-β-cyclodextrin inclusion complex / sodium hyaluronate prepared in Examples 1 to 3 and Comparative Examples 1 to 3 was measured. The nanofiber mask was dissolved in deionized water and prepared into test solutions of different concentrations. The mass volume ratio of the nanofiber mask in the test solution was 5 mg / mL, 10 mg / mL, 20 mg / mL and 30 mg / mL, respectively. The specific experimental steps are shown in Effect Implementation 5. The ABTS free radical scavenging rate is shown in Tables 5 and Figure 8 shown.

[0169] Table 5

[0170]

[0171] From Table 5 and Figure 8 It can be seen that the nanofiber membranes prepared in Examples 1 to 3 have better scavenging effects on ABTS free radicals than the nanofiber membranes prepared in Comparative Examples 1 to 3.

[0172] Effect Example 8

[0173] The experimental group selected 12 volunteers aged 18-60 who met the test requirements as test subjects. The nanofiber membrane prepared in Example 1 was cut into 5cm×5cm size, applied to the inner side of the forearm of the test subject, and sprayed with a small amount of deionized water to moisten it. The timing started from the dissolution of the nanofiber membrane, and the time when the subject felt heat was recorded within 30 minutes, and the skin was observed for irritation (obvious erythema, itching, swelling, tingling, etc.). The test results are shown in Table 6. Among them, "-" = no reaction; "+" = reaction.

[0174] Table 6

[0175]

[0176] From the results in Table 6, it can be seen that all volunteers felt the heat within 15 minutes, which had a heating effect and was in line with the normal use time of the mask (15-30 minutes). Among them, 4 volunteers among the 12 volunteers felt warm within 1 to 5 minutes, and 9 volunteers felt warm within 10 minutes, which shows that the nanofiber mask prepared in Example 1 has universal adaptability. In addition, the test skin of all volunteers did not show obvious irritation reactions such as skin redness, itching, and swelling.

[0177] Finally, it should be noted that in this application, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements that are not explicitly listed, or also includes elements that are inherent to such process, method, article or apparatus.

[0178] Although the present application has been disclosed above through the description of the specific embodiments of the present application, it should be understood that those skilled in the art can design various modifications, improvements or equivalents to the present application within the spirit and scope of the attached scheme. These modifications, improvements or equivalents should also be considered to be included in the scope of protection claimed in the present application.

Claims

1. A use of vanillyl butyl ether in a skin external preparation, characterized in that: Vanillyl butyl ether is used as an antioxidant active ingredient and exothermic ingredient in skin topical preparations; Wherein, the vanillyl butyl ether is added in an amount of 0.01 to 10 wt % in the skin external preparation.

2. The use of vanillyl butyl ether in a skin external preparation according to claim 1, characterized in that: The vanillyl butyl ether is included in the cyclodextrin to obtain a vanillyl butyl ether cyclodextrin inclusion compound, which is used in the skin external preparation; Preferably, the vanillyl butyl ether is added in an amount of 0.5 to 6 wt %, such as 1 wt % in the skin external preparation.

3. The use of vanillyl butyl ether in a skin external preparation according to claim 2, characterized in that: The preparation method of the vanillyl butyl ether cyclodextrin inclusion compound specifically comprises the following steps: (1) dissolving the vanillyl butyl ether in ethanol to prepare a vanillyl butyl ether alcohol solution; (2) dissolving the cyclodextrin in water and stirring until it is completely dissolved to prepare a cyclodextrin aqueous solution; (3) dropping the vanillyl butyl ether alcohol solution prepared in step (1) into the cyclodextrin aqueous solution prepared in step (2), stirring for inclusion, removing the solvent, and drying.

4. The use of vanillyl butyl ether in a skin external preparation according to claim 3, characterized in that: The preparation method of the vanillyl butyl ether cyclodextrin inclusion compound meets at least one of the following conditions: In step (1), the concentration of vanillyl butyl ether in the vanillyl butyl ether alcohol solution is 0.008 to 0.1 g / mL, preferably 0.03 to 0.05 g / mL; In step (1), the ethanol is anhydrous ethanol; In step (1), the stirring speed is 100 to 800 rpm, preferably 300 to 400 rpm; In step (1), the stirring time is 60 to 120 minutes, preferably 90 minutes.

5. The use of vanillyl butyl ether in a skin external preparation according to claim 3, characterized in that: The preparation method of the vanillyl butyl ether cyclodextrin inclusion compound meets at least one of the following conditions: In step (2), the concentration of cyclodextrin in the cyclodextrin aqueous solution is 0.007 to 0.5 g / mL, preferably 0.045 to 0.055 g / mL; In step (2), the stirring speed is 100 to 800 rpm; preferably 300 to 400 rpm; In step (2), the stirring time is 60 to 120 minutes, preferably 90 minutes; In step (2), the cyclodextrin includes hydroxypropyl-β-cyclodextrin, sulfobutyl-β-cyclodextrin, methyl-β-cyclodextrin, preferably hydroxypropyl-β-cyclodextrin; In step (3), after the vanillyl butyl ether alcohol solution is mixed with the cyclodextrin aqueous solution, the molar ratio of the vanillyl butyl ether to the cyclodextrin is 1:(1-2); In step (3), the dripping rate is 1 to 5 mL / min, for example 3 mL / min; In step (3), the stirring speed is 100 to 800 rpm, preferably 300 to 400 rpm; In step (3), the stirring time is 6 to 24 hours, preferably 12 hours.

6. Use of vanillyl butyl ether in a skin external preparation according to any one of claims 3 to 5, characterized in that: The preparation method of the vanillyl butyl ether cyclodextrin inclusion compound meets at least one of the following conditions: The method for removing the solvent includes reduced pressure evaporation and / or freeze drying. When the reduced pressure evaporation method is adopted, the temperature of the reduced pressure evaporation is 35 to 45° C.; wherein the time of the reduced pressure evaporation is 20 to 45 minutes; The drying is freeze drying. When the freeze drying is used for the drying, it also includes pre-freezing the sample. Preferably, the pre-freezing temperature is -30 to -10°C, more preferably -20°C; preferably, the pre-freezing time is 2 to 24 hours, preferably 12 hours; when the freeze drying is used for the drying, the freeze drying temperature is -90 to -70°C, preferably -80°C; when the freeze drying is used for the drying, the freeze drying time is 24 to 72 hours, preferably 48 hours.

7. Use of vanillyl butyl ether in a skin external preparation according to claim 1 or 2, characterized in that: The vanillyl butyl ether is used as an antioxidant active ingredient in the skin external preparation having an ABTS free radical scavenging effect and / or a DPPH free radical scavenging effect; Preferably, the vanillyl butyl ether cyclodextrin inclusion compound is used as an antioxidant active ingredient having ABTS free radical scavenging effect and / or DPPH free radical scavenging effect in the skin external preparation; Preferably, the vanillyl butyl ether or the vanillyl butyl ether cyclodextrin inclusion compound is used as the exothermic effect component in the skin external preparation, and more preferably, the exothermic effect component exerts an exothermic effect within 1 to 15 minutes and lasts for 15 to 30 minutes; Preferably, the skin external preparation includes a facial mask, lotion or cream, more preferably a facial mask, such as a nanofiber facial mask.

8. The use of vanillyl butyl ether in a skin external preparation according to claim 7, characterized in that: The nanofiber mask is prepared by an electrostatic spinning method, and the electrostatic spinning method specifically comprises the following steps: I. Compounding the vanillyl butyl ether cyclodextrin inclusion compound and the functional ingredients in a polymer spinning aid solution, stirring, and ultrasonically treating to obtain a spinning solution; II. Fix the spinning solution and spray it into the receiving device.

9. The use of vanillyl butyl ether in a skin external preparation according to claim 8, characterized in that: The electrospinning preparation method meets at least one of the following conditions: In step (I), the mass concentration of the vanillyl butyl ether cyclodextrin inclusion complex is 0.1 to 20 wt%, preferably 2 to 5 wt%; In step (I), the polymer spinning aid solution includes at least one of polyvinyl alcohol, polyethylene glycol, polyvinyl pyrrolidone, pullulan, pectin, gelatin, gum arabic and sodium alginate; In step (I), the concentration of the polymer spinning aid solution is 5 to 50 wt%, preferably 8 to 20 wt%; In step (I), the functional ingredients further include at least one of a moisturizing active ingredient, a whitening active ingredient, an anti-inflammatory active ingredient, an anti-allergic active ingredient, and an antioxidant active ingredient; preferably, the moisturizing active ingredient includes sodium hyaluronate, and more preferably, when the sodium hyaluronate is added, the concentration of the sodium hyaluronate is 0.5 to 10 wt%, preferably 1 to 4 wt%; In step (I), the stirring time is 2 to 24 hours, preferably 12 hours; In step (I), the ultrasonic treatment is an ultrasonic degassing treatment. Preferably, when the ultrasonic degassing treatment is used, the ultrasonic time is 10 to 20 minutes, more preferably 15 minutes; preferably, when the ultrasonic degassing treatment is used, the ultrasonic frequency is 150 to 300W, more preferably 200 to 250W; In step (II), the fixing is performed on an electrospinning device. Preferably, when the fixing is performed on the electrospinning device, the fixing voltage is 9 to 20 KV, more preferably 12 to 16 KV, for example 14 to 15 KV; In step (II), the spinning solution is sucked by a syringe and sprayed under the non-woven mask; preferably, the flow rate of the spraying is 0.2-0.6 mL / h, more preferably 0.3-0.4 mL / h; preferably, the spraying time is 1-20 h, more preferably 4-8 h; preferably, the receiving distance of the spraying is 5-30 cm, more preferably 15-20 cm, for example 15 cm.

10. A nanofiber facial mask of vanillyl butyl ether cyclodextrin inclusion complex, which is prepared by the electrospinning preparation method according to claim 8 or 9.

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