Traditional Chinese medicine composition for treating atopic dermatitis and preparation method thereof

Abstract

The invention relates to a traditional Chinese medicine composition for treating atopic dermatitis and a preparation method thereof, and the traditional Chinese medicine composition comprises the following components in parts by weight: 6-15 parts of honeysuckle, 6-15 parts of raw Chinese yam, 6-15 parts of cortex lycii radicis, 6-15 parts of prepared rhizoma polygonati, 6-15 parts of glossy privet fruit and 6-15 parts of coix seed. According to the treatment principle of eliminating dampness and heat and tonifying spleen and kidney, honeysuckle in the formula is long for clearing away heat and toxic materials, Chinese yam can tonify spleen and stomach and benefit lung and kidney, and honeysuckle and Chinese yam are used for clearing heat and promoting diuresis and tonifying spleen and kidney and serve as monarch drugs together. The heat clearing effects of the cortex lycii radicis and the honeysuckle are complementary, the kidney tonifying, yin nourishing, spleen tonifying and lung moistening effects of the rhizoma polygonati and the glossy privet fruit are complementary with the Chinese yam, the cortex lycii radicis, the rhizoma polygonati and the glossy privet fruit are jointly used as ministerial drugs, and the coix seeds are used as adjuvant drugs for clearing damp, promoting diuresis and tonifying spleen, so that the whole formula is mainly used for eliminating dampness and heat and tonifying spleen and kidney; experimental results show that all the medicines in the formula cooperate to achieve the effects of eliminating dampness and heat, invigorating the spleen and tonifying the kidney, and the formula also has a remarkable curative effect on treating atopic dermatitis through external application.

Description

Technical Field

[0001] The invention belongs to the field of skin disease treatment, and particularly relates to a traditional Chinese medicine composition for treating atopic dermatitis and a preparation method thereof. Background Art

[0002] Atopic dermatitis is the inflammatory skin disease with the highest incidence rate, especially in children, the prevalence of atopic dermatitis can reach 10% to 20%. Atopic dermatitis is clinically manifested by skin symptoms such as epidermal exfoliation, severe itching and lichenification, mainly involving the flexion area, face, neck and distal limbs, and some are accompanied by diseases such as asthma and allergic rhinitis. Due to the long duration of atopic dermatitis, repeated attacks and protracted unhealing, it often has a serious impact on the patient's life and psychology. Existing clinical treatments for atopic dermatitis include glucocorticoids, calcineurin inhibitors, biological agents and physical therapy, which mainly reduce the frequency of acute atopic dermatitis, delay the recurrence time interval and reduce the degree of itching to achieve disease relief. After long-term use of these drugs, capillary dilation, drug withdrawal rebound and other phenomena will occur, and even secondary bacterial or fungal infections will cause side effects such as folliculitis or tinea. Therefore, it is urgent to develop new drugs with long-lasting efficacy and few side effects.

[0003] Traditional Chinese medicine has always played an important role in the prevention and treatment of atopic dermatitis. Atopic dermatitis belongs to the category of eczema in the field of traditional Chinese medicine. Traditional Chinese medicine believes that eczema is caused by the body's intolerance, combined with rheumatism and heat evil, which accumulates in the skin. According to the patient's clinical manifestations, it can be divided into damp-heat infiltration syndrome, spleen deficiency and dampness accumulation syndrome, and blood deficiency and wind dryness syndrome. Therefore, under the guidance of the holistic concept and the theory of syndrome differentiation and treatment, traditional Chinese medicine uses the principles of clearing heat, promoting dampness, and strengthening the spleen to treat eczema, which has obvious clinical advantages. Traditional Chinese medicine has a rich means of treating eczema. Before the Ming and Qing Dynasties, doctors classified eczema as "external ulcers" and mainly treated it with external treatment. Since the Ming and Qing Dynasties, doctors in later generations have begun to pay attention to internal causes as the basis of eczema on the basis of summarizing the experience of their predecessors, especially the internal treatment strategy. They usually treat external sores and ulcers with Chinese medicine taken internally, which is the internal treatment method. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a Chinese medicine composition for treating atopic dermatitis and a preparation method thereof, which has significant efficacy, high safety, and is convenient and fast.

[0005] The invention provides a traditional Chinese medicine composition for treating atopic dermatitis. The composition comprises the following components by weight: 6-15 parts of honeysuckle, 6-15 parts of raw yam, 6-15 parts of lycium bark, 6-15 parts of processed polygonatum, 6-15 parts of glossy privet fruit and 6-15 parts of coix seeds.

[0006] Preferably, the mass ratio of the honeysuckle, raw yam, cortex lycii, processed polygonatum, fructus ligustri lucidi and semen coicis is 1:1:1:1:1:1:1.

[0007] The present invention also provides a method for preparing a Chinese medicine composition for treating atopic dermatitis, comprising:

[0008] Take the raw material medicine by weight, add water and boil for 1-3 times, the first time adding water is 6-10 times the total weight of the raw material medicine, boiling for 1.5-2.5 hours, the second and third time adding water is 6-10 times the total weight of the raw material medicine, boiling for 0.5-1.5 hours, combining the decoctions and filtering to obtain the filtrate.

[0009] The amount of water added is further preferably 7-9 times the total weight of the API, more preferably 8 times.

[0010] The number of times of decocting with water is preferably 2 times, the first decocting is 1.5 to 2.5 hours, and the second decocting is 0.5 to 1.5 hours.

[0011] In the present invention, after decoction extraction, the extract is concentrated. The extract of the present invention is preferably obtained by filtration, and the concentration is preferably concentrated at 80°C to a relative density of 1.34. After concentration, it is preferably allowed to stand at room temperature, and the supernatant is taken to obtain the traditional Chinese medicine composition.

[0012] The present invention also provides a traditional Chinese medicine for treating atopic dermatitis, comprising active ingredients of the traditional Chinese medicine composition and pharmaceutically acceptable adjuvants.

[0013] Preferably, the dosage form of the drug includes one or more of granules, lotions, gels, and patches.

[0014] Preferably, the auxiliary material includes one or more of dextrin, carbomer and triethanolamine.

[0015] When the Chinese medicine composition of the present invention is a granule, it is taken orally, 1 to 2 times a day, and each bag (5g / bag) is dissolved in 50mL of warm water and then taken orally. When the Chinese medicine composition of the present invention is a lotion, the usage and dosage of the Chinese medicine composition lotion is external use, 1 to 2 times a day. When the Chinese medicine composition of the present invention is a gel, it is external use, 1 to 2 times a day, at 2 to 3 mg / cm 2 Apply the dose to the affected area.

[0016] The invention provides a Chinese medicine composition for treating atopic dermatitis. The Chinese medicine composition is formulated according to the principle of "dialectical reasoning, and treatment based on reason" in traditional Chinese medicine, and has the effects of clearing dampness and heat, strengthening the spleen and benefiting the kidney, and is suitable for skin diseases caused by dampness and heat blocking the middle jiao. In the prescription, honeysuckle is sweet and cold in nature; it enters the lung, heart, and stomach meridians, has the effects of clearing heat and detoxifying, and evacuating wind-heat, and is commonly used in the clinic to treat carbuncle, furuncle, and is the key medicine for treating all internal and external carbuncle; raw yam is sweet and flat in nature, enters the spleen, lung, and kidney meridians, has the effects of tonifying the spleen and stomach, benefiting the lung and kidney, and has the effects of invigorating qi and nourishing yin, and tonifying the spleen, lung, and kidney; honeysuckle and raw yam clear heat and dampness, strengthen the spleen and benefit the kidney, and both are monarch medicines. The heat-clearing effect of the root bark of Rehmannia glutinosa and honeysuckle is complementary, and the kidney-tonifying and yang-nourishing, spleen-tonifying and lung-moistening effects of Polygonatum sibiricum and Fructus Ligustri Lucidi are complementary to raw yam, and are the ministerial medicines of this prescription. The adjuvant of this prescription is coix seed, which is sweet, mild and slightly cold in nature, has the effect of promoting diuresis, eliminating dampness and strengthening the spleen, can not only drain fire from the bladder, but also can harmonize the effects of various medicines. The combination of various medicines has good effects of clearing dampness and heat, strengthening the spleen and kidney, and has good curative effect in treating atopic dermatitis. In addition, the preparation method of the present invention is simple, easy to operate, easy to use, low in cost, and more easily accepted by patients.

[0017] Beneficial Effects

[0018] 1. The present invention is rigorous in formulating prescriptions and treating the disease based on the cause. Traditional Chinese medicine believes that eczema is caused by the body's inherent intolerance, combined with rheumatic heat evil, and accumulation in the skin. In the prescription, honeysuckle is sweet and cold in nature; it enters the lung, heart, and stomach meridians, has the effects of clearing away heat and detoxifying, and evacuating wind-heat. It is commonly used in the clinic to treat carbuncles, furuncles, and is the key medicine for treating all internal and external carbuncles; raw yam is sweet and flat in nature, enters the spleen, lung, and kidney meridians. It has the effects of tonifying the spleen and stomach, benefiting the lungs and kidneys, and has the effects of invigorating qi and nourishing yin, and tonifying the spleen, lungs, and kidneys; honeysuckle and raw yam clear away heat and dampness, strengthen the spleen and benefit the kidneys, and both are monarch drugs. The heat-clearing effects of Rehmannia root bark and honeysuckle are complementary, and the kidney-tonifying and yang-nourishing, spleen-tonifying and lung-moistening effects of Polygonatum sibiricum and Fructus Ligustri Lucidi are complementary to raw yam, which are the ministerial drugs of this prescription. The adjuvant of this prescription is coix seed, which is sweet, mild and slightly cold in nature. It has the effect of promoting diuresis, removing dampness and strengthening the spleen. It can not only drain fire from the bladder, but also harmonize the effects of various medicines. The combination of various medicines has a good effect of clearing dampness and heat, strengthening the spleen and kidney. It has a good effect in treating dry eczema or asthma.

[0019] 2. The granules of the Chinese medicine composition of the present invention are clinical characteristic Chinese medicine preparations, which are derived from the summary of many years of clinical experience of old Chinese medicine practitioners. The prescription is rigorous and reasonable, the preparation process is stable, the product quality is controllable, it has many years of clinical application experience, definite efficacy, no obvious adverse reactions, and has significant prospects for new drug transformation.

[0020] 3. Decoction is the most traditional and commonly used method of traditional Chinese medicine, but there is no optimal control standard for its process, which often leads to significant differences in the efficacy of the drug. The present invention compares the effects of water extraction, alcohol extraction and water extraction and alcohol precipitation processes on the efficacy of particles through pharmacodynamic tests to determine the extraction and purification process. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a statistical chart of changes in SCORAD scores.

[0022] Figure 2 This is a statistical chart of weight changes.

[0023] Figure 3 The following are the differences in AD phenotypes among the mice in each group.

[0024] Figure 4 Statistical graph of serum TSLP levels in each group of mice.

[0025] Figure 5 Statistical graph of serum IgE levels in each group of mice.

[0026] Figure 6 Statistical graph of serum IFN-γ levels in each group of mice.

[0027] Figure 7 Statistical graph of serum IL-4 levels in each group of mice.

[0028] Figure 8 Statistical graphs of the stratum corneum of HE-stained sections of mice in each group.

[0029] Fig. 9 The figure is a statistical chart of the number of mast cells stained with toluidine blue in each group of mice.

[0030] Fig.10 The HE staining differences of mouse tissues in each group.

[0031] Fig.11 The difference diagram of toluidine blue staining of mouse tissues in each group. DETAILED DESCRIPTION

[0032] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the appended claims of the application equally.

[0033] In the present invention, unless otherwise specified, all raw material components are commercially available products well known to those skilled in the art.

[0034] Example 1

[0035] A Chinese medicine composition (A) for treating atopic dermatitis, comprising the following raw materials of the following quality:

[0036] 15g honeysuckle, 15g raw yam, 15g Rehmannia root bark, 15g processed Polygonatum sibiricum, 15g Ligustrum lucidum fruit, 15g Coix seed.

[0037] The preparation method of the above-mentioned Chinese medicine composition for treating atopic dermatitis comprises the following steps:

[0038] Take the above-mentioned prescription amount of raw materials, add 8 times the total weight of the raw materials, i.e. 720 ml of water, and boil for 2 hours. Then take 6 times the total weight of the raw materials, i.e. 540 ml of water, and boil for 1 hour. Filter and take the supernatant, combine the decoctions, concentrate at 80°C to 80 ml, let stand at room temperature for 24 hours, and take the supernatant.

[0039] Comparative Example 1

[0040] A Chinese medicine composition (B) for treating atopic dermatitis, comprising the following raw materials of the following quality:

[0041] Honeysuckle 15g, Rehmannia root bark 15g, processed Polygonatum 15g, Ligustrum lucidum fruit 15g, Coix seed 15g.

[0042] The preparation method of the above-mentioned Chinese medicine composition for treating atopic dermatitis comprises the following steps:

[0043] Take the above-mentioned prescription amount of raw materials, add 8 times the total weight of the raw materials, i.e. 600 ml of water, and boil for 2 hours. Then take 6 times the total weight of the raw materials, i.e. 450 ml of water, and boil for 1 hour. Filter and take the supernatant, combine the decoctions, concentrate at 80°C to 65 ml, let stand at room temperature for 24 hours, take the supernatant, and you have it.

[0044] Comparative Example 2

[0045] A Chinese medicine composition (C) for treating atopic dermatitis, comprising the following raw materials of the following quality:

[0046] Honeysuckle 15g, raw yam 15g, processed polygonatum 15g, Ligustrum lucidum 15g, coix seed 15g.

[0047] Add 8 times the total weight of the raw material to the above-mentioned prescription amount of raw materials, i.e. 600 ml of water, and boil for 2 hours. Then take 6 times the total weight of the raw material, i.e. 450 ml of water, and boil for 1 hour. Filter and take the supernatant. Combine the decoctions, concentrate at 80°C to 65 ml, let stand at room temperature for 24 hours, and take the supernatant.

[0048] Comparative Example 3

[0049] A Chinese medicine composition (D) for treating atopic dermatitis, comprising the following raw materials of the following quality:

[0050] Honeysuckle 15g, raw yam 15g, Rehmannia root bark 15g, processed Polygonatum 15g, Ligustrum lucidum 15g.

[0051] Take the above-mentioned prescription amount of raw materials, add 8 times the total weight of the raw materials, i.e. 600 ml of water, and boil for 2 hours. Then take 6 times the total weight of the raw materials, i.e. 450 ml of water, and boil for 1 hour. Filter and take the supernatant, combine the decoctions, concentrate at 80°C to 65 ml, let stand at room temperature for 24 hours, take the supernatant, and you have it.

[0052] Comparative Example 4

[0053] A Chinese medicine composition (E) for treating atopic dermatitis, comprising the following raw materials of the following quality:

[0054] Honeysuckle 15g, raw yam 15g, Rehmannia root bark 15g, Ligustrum lucidum fruit 15g, Coix seed 15g.

[0055] Take the above-mentioned prescription amount of raw materials, add 8 times the total weight of the raw materials, i.e. 600 ml of water, and boil for 2 hours. Then take 6 times the total weight of the raw materials, i.e. 450 ml of water, and boil for 1 hour. Filter and take the supernatant, combine the decoctions, concentrate at 80°C to 65 ml, let stand at room temperature for 24 hours, take the supernatant, and you have it.

[0056] Preparation Example 1

[0057] A Chinese medicine composition (F) for treating atopic dermatitis is composed of the following raw materials:

[0058] 30g honeysuckle, 30g raw yam, 30g Rehmannia root bark, 30g processed Polygonatum sibiricum, 30g Ligustrum lucidum fruit, 30g Coix seed.

[0059] Take the above-prescribed raw materials, add 8 times the total weight of the raw materials, i.e. 1440 ml of water, and boil for 2 hours. Then take 6 times the total weight of the raw materials, i.e. 1080 ml of water, and boil for 1 hour. Filter and take the supernatant, combine the decoctions, concentrate at 80°C to a relative density of 1.34, let stand at room temperature for 24 hours, take the supernatant, add purified water to 1000 mL, stir well, and divide into packages.

[0060] The specification of the Chinese medicine composition lotion is 250 mL / bag.

[0061] Preparation Example 2

[0062] A Chinese medicine composition (G) for treating atopic dermatitis, comprising the following raw materials of the following quality:

[0063] 300g honeysuckle, 300g raw yam, 300g Rehmannia root bark, 300g processed Polygonatum sibiricum, 300g Ligustrum lucidum fruit, 300g Coix seed.

[0064] Take the above-mentioned prescription amount of raw materials, add 8 times the total weight of the raw material water, i.e. 1440 ml, decoct for 2 hours, then take 6 times the total weight of the raw material water, i.e. 1080 ml, decoct for 1 hour, filter out the supernatant, combine the decoctions, concentrate at 80°C to an extract with a relative density of 1.34, add 3 times the amount of dextrin, mix well, granulate, dry, and granulate to make 1000g of granules.

[0065] The specification of the Chinese medicine composition granules is 5g / bag.

[0066] Preparation Example 3

[0067] A Chinese medicine composition (H) for treating atopic dermatitis, comprising the following raw materials of the following quality:

[0068] 15g honeysuckle, 15g raw yam, 15g Rehmannia root bark, 15g processed Polygonatum sibiricum, 15g Ligustrum lucidum fruit, 15g Coix seed.

[0069] The preparation method of the above-mentioned Chinese medicine composition for treating atopic dermatitis comprises the following steps:

[0070] Take the above-mentioned prescription amount of raw materials, add 8 times the total weight of the raw material, that is, 720 ml of water, decoct for 2 hours, then take 6 times the total weight of the raw material, that is, 540 ml of water, decoct for 1 hour, filter out the supernatant, combine the decoctions, concentrate at 80°C to an extract with a relative density of 1.34, let stand at room temperature for 24 hours, add 0.8g of carbomer 941, swell and stir evenly, add appropriate amount of triethanolamine to adjust the pH to neutral, add purified water to 80g, and stir evenly to obtain.

[0071] Test Example 1

[0072] Experimental animals: BALB / C mice, male, 8-9 weeks old, 23±1g.

[0073] Preparation groups: blank control group (skin untreated group), model group (only given calcipotriol), Chinese medicine composition group A, Chinese medicine composition group B, Chinese medicine composition group C, Chinese medicine composition group D, and Chinese medicine composition group E.

[0074] Preparation groups: Example 1 is used as Chinese medicine composition group A. Control example 1 is used as Chinese medicine composition group B. Control example 2 is used as Chinese medicine composition group C. Control example 3 is used as Chinese medicine composition group D, and control example 4 is used as Chinese medicine composition group E.

[0075] Experimental process: The mouse skin atopic dermatitis model was established by calcipotriol induction method, and the specific method was as follows: 60 9-week-old Balb / c mice were randomly divided into 6 groups, 10 mice in each group, namely blank control group, model group, Chinese medicine composition group A, Chinese medicine composition group B, Chinese medicine composition group C, Chinese medicine composition group D and Chinese medicine composition group E. On the morning of the first day (Day 0), 2nmol calcipotriol ethanol solution was given to each mouse in both ears except the blank control group. From the second day (Day 1), at 9:00 a.m. every day, 20 μl of anhydrous ethanol was applied to the ears of the mice in the blank control group, and 20 μl of calcipotriol ethanol solution was applied to the ears of the mice in the model group, the Chinese medicine composition group A, the Chinese medicine composition group B, the Chinese medicine composition group C, the Chinese medicine composition group D and the Chinese medicine composition group E to ensure that the dosage of the modeling agent reached 2nmol / ear to create an eczema model; at 17:00 p.m., the mice in the blank control group and the model group were gavaged with 0.1 ml of purified water, and the mice in the Chinese medicine composition group A, the Chinese medicine composition group B, the Chinese medicine composition group C, the Chinese medicine composition group D and the Chinese medicine composition group E were gavaged with 0.1 ml of Chinese medicine composition A extract, Chinese medicine composition B extract, Chinese medicine composition C extract, Chinese medicine composition D extract and Chinese medicine composition E extract, respectively, for 5 days. Photos were taken every day, and the severity of the skin lesions in the ear application area of ​​the mice was evaluated by the atopic dermatitis score (SCORAD). After the last administration (Day 5), the condition of the damaged skin tissue was observed. No modeling stimulation was performed from Day 6 to Day 7. The afternoon of Day 7 was the peak state of atopic dermatitis. The damaged skin tissue was obtained and homogenate was prepared. The atopic dermatitis-related indicators of the damaged skin tissue of each group of mice were detected, and the diseased skin tissue was observed by tissue section staining.

[0076] The SCORAD items include (i) pruritus / itching, (ii) erythema / hemorrhage, (iii) edema, (iv) epidermis / erosion, and (v) scaling / dryness. Each symptom is graded as follows: 0 (no symptoms), 1 (mild), 2 (moderate), and 3 (severe). The total dermatitis score ranges from 0 to 15 points.

[0077] All data are presented as mean ± standard deviation (SD). Data were analyzed using one-way analysis of variance (ANOVA) or t-test (two-tailed). Statistical analysis was performed using GraphPad Pris m (v 8.0). P values ​​less than 0.05 were considered statistically significant (*p<0.05, **p<0.01, and ***p<0.001).

[0078] 1. Atopic dermatitis level:

[0079] Table 1. SCORAD score change table

[0080]

[0081]

[0082] Table 2. Mouse body weight changes

[0083]

[0084] Table 3. Statistics of ear thickness of mice in each group on Day 7 (n=10)

[0085]

[0086] like Figure 1-Figure 3 As shown in Tables 1-3:

[0087] On the third day of administration, except for the normal control group, the ears of mice in each group given the modeling agent showed varying degrees of eczema symptoms, such as redness and swelling of both ears and scratching behavior. The ears of mice with severe eczema showed erythema and vascular dilation inflammation characteristics, and all mice did not show obvious desquamation. In addition, except for the normal control group, the weight of mice in each group decreased to varying degrees, but the weight loss rate of mice in group A after Chinese medicine was slower than that of the model group. SCORAD scores were performed on each group of mice, and the results showed that compared with the normal control group, the scores of mice in each group increased, and the scores were close to no significant difference.

[0088] On the 5th day of administration, the hair of mice in the normal control group was dense, while the hair of mice in the other groups was sparse, and their bodies were curled to varying degrees. The ears of mice in the model group and the Chinese medicine BCDE group generally showed more severe eczema lesions. The mice in the Chinese medicine A group had eczema lesions, but there was no significant difference. The weight of mice treated with the modeling agent continued to decrease. The weight loss rate of mice in the model group and BCDE group was 15%-20%, and the weight loss rate of mice in the Chinese medicine A group was concentrated in the range of 10%-15%, with no statistical difference. The SCORAD score was performed on each group of mice, and the results showed that the score of mice in the model group was higher than that of other groups, and the SOCR atopic dermatitis score of the Chinese medicine A group was close, with no significant difference.

[0089] On the 7th day of administration, the thickening degree of eczema lesions in the ears of mice in the model group and the Chinese medicine BCDE group was concentrated at 1.5±0.1 times, among which the ears of mice with severe eczema symptoms in the model group showed desquamation symptoms. The thickening degree of the ears of mice in the Chinese medicine A group was concentrated at 1.2±0.1 times, which was statistically different from the model group. The SCORAD score of each group of mice showed that the score of the blank control group mice did not change during the experiment, and the score of the model group and the Chinese medicine BCDE group mice was higher than that of the Chinese medicine A group.

[0090] The results showed that ① the composition of Chinese medicine group A had excellent therapeutic effect on eczema and could improve the animal model of eczema; ② the anti-eczema effect of the composition of Chinese medicine groups BCDE was close to that of the model group, and it was considered that Chinese medicine groups BCDE had no obvious therapeutic effect on eczema; ③ the prescription of Chinese medicine group A consisted of honeysuckle, raw yam, radix rehmanniae, processed polygonatum, fructus ligustris and coix seed, the prescription of Chinese medicine group B was based on the prescription of group A except that raw yam was removed, the prescription of Chinese medicine group C was based on the prescription of group A except that cortex rehmanniae, the prescription of Chinese medicine group D was based on the prescription of group A except that coix seed was removed, and the prescription of Chinese medicine group E was based on the prescription of group A except that processed polygonatum was removed. None of the four groups BCDE showed therapeutic effect on eczema, indicating that the Chinese medicine prescription of the present invention has good anti-inflammatory effect.

[0091] 2. Serum biochemical examination

[0092] Table 4. Statistical table of serum biochemical indexes (n=10)

[0093]

[0094] like Figure 4-Figure 7 As shown in Table 4, compared with the normal control group, the serum inflammatory factor concentrations of mice in the model control group and the Chinese medicine BCDE groups were significantly increased, among which the inflammatory factor concentrations of mice treated with the Chinese medicine group A prescription were lower than those of the model control group, including TSLP, IgE, IFN-γ, and IL-4. This indicates that the Chinese medicine group A prescription has a significant improvement effect on the serum inflammatory factor concentrations of eczema model mice. The Chinese medicine BCDE respectively removes raw yam, Chinese herbal medicine root bark, coix seed, and processed polygonatum in the prescription of the present invention, and the anti-inflammatory effect is greatly weakened, indicating that the eczema treatment of the prescription of the present invention is complemented by the 6 medicinal materials.

[0095] 3. Pathological examination

[0096] Table 5. Statistical table of stratum corneum thickness in HE-stained sections (×100 μm, n=10)

[0097] Group Dosage (g / ml) Animals Stratum corneum thickness (μm) Blank control group / 10 35.3±4.2 Model control group / 10 90.9±11.5 Chinese Medicine Group A 5.487 10 43.2±4.0 Chinese medicine group B 4.572 10 68.2±3.9 Chinese medicine group C 4.572 10 66.3±6.9 Chinese medicine group D 4.572 10 74.1±2.6 Chinese Medicine Group E 4.572 10 72.6±3.2

[0098] Table 6. Statistics of mast cell numbers in toluidine blue stained sections (×100 μm, n=10)

[0099]

[0100] The results of HE pathological examination are shown in Table 5 and Figure 8 , Fig.10As shown: Compared with the normal control group, the epidermal keratinization, incomplete keratinization, thickening of the stratum spinosum, hyperplasia of the granular layer, and a large number of inflammatory cells and eosinophils infiltrated the dermis in the model group and the Chinese medicine BCDE group. Compared with the pathological sections of the model group, the thickening of the stratum spinosum of the mice in the Chinese medicine A group was significantly alleviated, and the inflammatory cells and eosinophils in the dermis were greatly reduced. Since the Chinese medicines BCDE respectively remove raw yam, lycium bark, coix seed and processed polygonatum in the prescription of the present invention, it is believed that raw yam, lycium bark, coix seed and processed polygonatum have important anti-inflammatory effects in the prescription of the present invention, indicating the effectiveness of the prescription of the present invention.

[0101] The results of toluidine blue pathological examination are shown in Table 6 and Fig. 9 , Fig.11 The results showed that compared with the normal control group, a large number of mast cell infiltrations were found in the skin lesions of the model group and the Chinese medicine BCDE groups. Compared with the pathological sections of the model group, the number of mast cell infiltrations in the mice of the Chinese medicine A group was greatly reduced, proving that the raw yam, the root bark of Lycium bark, the coix seed and the processed polygonatum sibiricum in the prescription of the present invention have important anti-inflammatory effects, indicating the effectiveness of the prescription of the present invention in treating eczema.

Claims

1. A Chinese medicine composition for treating atopic dermatitis, characterized in that: The invention comprises the following components by weight: 6-15 parts of honeysuckle, 6-15 parts of raw yam, 6-15 parts of lycium bark, 6-15 parts of processed polygonatum, 6-15 parts of ligustrum lucidum fruit and 6-15 parts of coix seeds.

2. The Chinese medicine composition for treating atopic dermatitis according to claim 1, characterized in that: The mass ratio of the honeysuckle, raw yam, Chinese wolfberry bark, processed polygonatum, glossy privet fruit and coix seed is 1:1:1:1:1:1:

1.

3. A method for preparing a Chinese medicine composition for treating atopic dermatitis as claimed in any one of claims 1 to 2, comprising: Take the raw material medicine by weight, add water and boil for 1-3 times, the first time adding water is 6-10 times the total weight of the raw material medicine, boiling for 1.5-2.5 hours, the second and third time adding water is 6-10 times the total weight of the raw material medicine, boiling for 0.5-1.5 hours, combining the decoctions and filtering to obtain the filtrate.

4. A Chinese medicine for treating atopic dermatitis, characterized in that: The invention comprises the active ingredients of the Chinese medicine composition as claimed in any one of claims 1 to 2, and pharmaceutically acceptable excipients.

5. The Chinese medicine according to claim 4, characterized in that: The dosage form of the drug includes one or more of granules, lotions, gels, and patches.

6. The Chinese medicine according to claim 4, characterized in that: The auxiliary materials include one or more of dextrin, carbomer and triethanolamine.

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