Traditional Chinese medicine compound and preparation for reducing blood uric acid and relieving pain and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine compound and preparation for reducing blood uric acid and relieving pain and a preparation method of the traditional Chinese medicine compound, belongs to the technical field of traditional Chinese medicine compounds, and solves the technical problem that at present, a medicine which does not have side effects and is used for treating hyperuricemia is lacked. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 10-30 parts of cortex phellodendri, 5-20 parts of rhizoma coptidis, 15-30 parts of rhizoma atractylodis, 20-40 parts of semen coicis, 10-30 parts of radix cyathulae, 10-30 parts of pawpaw, 3-5 parts of fried fructus evodiae, 15-50 parts of radix astragali, 5-15 parts of The traditional Chinese medicine composition has the effects of reducing blood uric acid, resisting inflammation and relieving pain, is reasonable in compatibility, complements each other and has a synergistic effect, and the effects of reducing blood uric acid, resisting inflammation and relieving pain are increased.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine compound prescriptions, and in particular relates to a traditional Chinese medicine compound prescription for reducing blood uric acid and relieving pain, a preparation and a preparation method thereof. Background Art

[0002] Gout refers to a group of diseases in which uric acid in the blood is deposited in the joints and tissues, causing multiple damages. In severe cases, it may be complicated by cardiovascular and cerebrovascular diseases, renal failure, and may eventually be life-threatening. It is an independent risk factor for the occurrence of diseases such as diabetes, metabolic syndrome, dyslipidemia, chronic kidney disease, and stroke. The treatment of gout is divided into acute and remission phases. In the acute phase, non-steroidal analgesics, colchicine tablets, and glucocorticoids are mainly used for anti-inflammatory and analgesic treatment. In the remission phase, benzbromarone tablets, febuxostat tablets, and allopurinol tablets are mainly used for uric acid-lowering treatment. These drugs have many side effects in the treatment of gout, which may cause serious adverse reactions such as liver and kidney damage and gastrointestinal mucosal bleeding. In addition, some drugs are expensive, which brings great trouble to patients. Finding effective drugs to treat gout has become an urgent need for clinical treatment. Therefore, there is currently a lack of a drug that has no side effects to treat high uric acid levels. Summary of the invention

[0003] In order to solve the above technical problems, the present invention provides a Chinese medicine compound prescription for lowering blood uric acid and relieving pain, a preparation and a preparation method thereof. Salt-coptis bark and wine-coptis root are used as monarch drugs, which have strong effects of clearing away heat and dampness, purging fire and detoxifying, and have a direct therapeutic effect on symptoms of damp-heat accumulation such as gout; Atractylodes lancea and Coix seeds are used as assistant drugs, Atractylodes lancea strengthens the spleen, and Coix seeds promote diuresis, so as to assist the monarch drugs in strengthening the effects of clearing heat and drying dampness; Rhizoma Cyathulae, papaya, and stir-fried Evodia rutaecarpa are used as adjuvant drugs, which can promote blood circulation, remove blood stasis, relax muscles and tendons, dispel cold and relieve pain, and help relieve joint pain and other symptoms of gout patients; Raw Astragalus, Pheretima, and Corydalis are used as guiding drugs, Raw Astragalus replenishes qi and promotes diuresis, Pheretima promotes blood circulation and dredges meridians, and Corydalis promotes blood circulation and relieves pain, so as to guide other drugs to directly reach the diseased area and enhance the therapeutic effect.

[0004] The technical solution adopted by the present invention is as follows: A traditional Chinese medicine compound for lowering blood uric acid and relieving pain is composed of the following raw materials by weight: 10-30 parts of phellodendron, 5-20 parts of coptis, 15-30 parts of atractylodes, 20-40 parts of coix seeds, 10-30 parts of cyathula, 10-30 parts of papaya, 3-5 parts of stir-fried evodia rutaecarpa, 15-50 parts of astragalus, 5-15 parts of earthworm and 20-30 parts of yanhusuo.

[0005] Preferably, the Phellodendron chinense is salt Phellodendron chinense.

[0006] Preferably, the Coptis chinensis is Coptis chinensis fermented with wine.

[0007] Preferably, the papaya is raw papaya slices.

[0008] Preferably, the astragalus is raw astragalus.

[0009] A traditional Chinese medicine preparation for lowering blood uric acid and relieving pain is prepared using the traditional Chinese medicine compound described above, and includes decoctions, pills, powders and pastes.

[0010] A method for preparing a Chinese medicine preparation for lowering blood uric acid and relieving pain, which is used to prepare the above-mentioned decoction, comprises the following steps: Step 1: Mix salted Phellodendron, wine-soaked Coptis, Atractylodes, Coix seeds, Cyathula, papaya, stir-fried Evodia rutaecarpa, raw Astragalus, earthworm, and Corydalis according to weight, and soak in cold water for 30 to 60 minutes; Step 2: Boil the mixed medicine obtained after soaking in step 1, and keep boiling for 20 to 30 minutes; Step 3: filtering the mixture obtained in step 2 to obtain the first medicinal solution and the first medicinal residue; Step 4: Add cold water to the first batch of medicinal residues, bring to a boil and keep boiling for 20 to 30 minutes; Step 5: Filter the mixture obtained in step 4 to obtain a second medicinal solution and a second medicinal residue; Step 6: Mix the first solution with the second solution to obtain a dose of medicine.

[0011] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. The main ingredients of salted Phellodendron include berberine hydrochloride, alkaloids, tannins and various amino acids, which belong to the kidney meridian and bladder meridian, and have the effects of clearing away heat and dampness, purging fire and detoxifying, removing bone steaming and hot flashes, nourishing yin and reducing fire. The processed salted Phellodendron can enhance the efficacy of Phellodendron, making it more suitable for the treatment of hyperuricemia; Coptis chinensis itself has the effects of clearing heat and dampness, purging fire and detoxifying. After being made into wine-made Coptis chinensis, it is more suitable for patients with high uric acid constitution, and helps to regulate high uric acid caused by damp heat; Atractylodes can strengthen the spleen and eliminate dampness, and can be combined with salted Phellodendron to enhance the effect of clearing heat and drying dampness, and is suitable for hyperuricemia with dampness and turbidity accumulation; Coix seed has the effects of diuresis and dampness, strengthening the spleen and stopping diarrhea, and is suitable for symptoms such as dampness blocking the middle jiao and edema, which is indirectly beneficial It is used to control uric acid levels; Sichuan Achyranthes has the effects of promoting blood circulation, removing blood stasis, strengthening muscles and bones, and has a certain relieving effect on joint pain in gout patients; papaya can relax muscles and tendons, harmonize the stomach and remove dampness, and is suitable for symptoms such as rheumatic arthralgia and indigestion, and has a certain auxiliary effect on gout patients; fried Evodia rutaecarpa has the effects of dispelling cold and relieving pain, relieving adverse reactions and stopping vomiting, and is suitable for gout patients with cold and dampness arthralgia; raw Astragalus has the effects of replenishing Qi, consolidating the exterior, promoting diuresis and reducing swelling, and has an improving effect on symptoms such as edema caused by Qi deficiency, and indirectly promotes uric acid excretion; earthworms have the effects of promoting blood circulation, removing blood stasis, and unblocking meridians, and are suitable for gout patients with blood stasis blocking the meridians; Corydalis has the effects of promoting blood circulation, promoting Qi, and relieving pain, and is suitable for pain caused by Qi stagnation and blood stasis.

[0012] 2. Salted Phellodendron has a bitter and cold nature, and is especially good at clearing dampness and heat from the lower jiao. Wine-coptis has a bitter and cold taste, and can clear heat and dry dampness, purge fire and detoxify. It is the main drug together with salted Phellodendron; Atractylodes has a bitter and dry slightly warm nature, and can strengthen the spleen and eliminate dampness, treat both the symptoms and the root cause, and is the ministerial drug; they are used together, salted Phellodendron and wine-coptis are good at clearing heat, and Atractylodes is good at drying dampness, with unique effects, and the taste of the drugs is controlled to make the drugs mild to prevent damage to the stomach qi. Coix seed has a sweet and mild nature, and can penetrate dampness and remove arthralgia, strengthen the spleen and replenish the middle, discharge pus and eliminate carbuncle, relax muscles and relieve urgency, and clear heat and detoxify. Papaya has a sour and warm nature, and can relax muscles and activate collaterals, harmonize the stomach and eliminate dampness. Sichuan Cyathula is bitter and sweet in nature, and can promote blood circulation and menstruation, remove blood stasis and relieve pain, nourish the liver and kidney, strengthen tendons and bones, promote diuresis, and guide blood downward, so it is used as a guiding drug; fried Evodia rutaecarpa is bitter, pungent and hot, and can dispel cold and relieve pain, reduce adverse reactions and stop vomiting, and can counteract the bitter and cold stomach-damaging nature of salted Phellodendron. Raw Astragalus is sweet and slightly warm in nature, and can replenish qi and strengthen the spleen, promote diuresis and reduce swelling. If the spleen is healthy, dampness can be eliminated. Raw Astragalus is paired with Coix seeds to enhance the effect of strengthening the spleen and removing dampness. Pheretima is salty and cold in nature, and is good at clearing heat and calming the liver, and unblocking the meridians. "Compendium of Materia Medica" points out that "its nature is cold and descends, so it can relieve various heat diseases, and it descends so it can promote urination, treat foot diseases and dredge the meridians." It is cold in nature so it can treat heat diseases, and it tends to descend so it can promote urination, treat foot diseases, and dredge the meridians. The compatibility of earthworm with salt phellodendron and wine coptis can enhance the effect of clearing away heat, and with Sichuan cyathula, it tends to go straight down, making it easier for the medicine to reach the diseased foot. Corydalis yanhusuo is pungent, bitter and warm in nature, and has the effects of promoting qi and blood circulation and relieving pain. DETAILED DESCRIPTION

[0013] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution of the present application will be clearly and completely described below in combination with the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

[0014] Embodiment 1~5 Step 1: Mix salted Phellodendron, wine-scented Coptis, Atractylodes, Coix seeds, Cyathula, papaya, stir-fried Evodia rutaecarpa, raw Astragalus, earthworm, and Corydalis according to the weight parts shown in Table 1, and soak in cold water for 30 minutes; Step 2: Boil the mixed medicine obtained after soaking in step 1 and keep boiling for 20 minutes; Step 3: filtering the mixture obtained in step 2 to obtain the first medicinal solution and the first medicinal residue; Step 4: Add cold water to the first batch of medicinal residues, bring to a boil and keep boiling for 20 minutes; Step 5: Filter the mixture obtained in step 4 to obtain a second medicinal solution and a second medicinal residue; Step 6: Mix the first solution with the second solution to obtain a dose of medicine.

[0015] Table 1: Weight of each drug added in a traditional Chinese medicine compound for lowering blood uric acid and relieving pain

[0016] Rat experiments: (1) 100 rats weighing 18-22 g were divided into 5 groups, corresponding to the experimental groups in Examples 1 to 5, with 20 rats in each group; (2) A rat model of hyperuricemia and its complications was established using potassium oxonate (OA) combined with hypoxanthine (H); (3) The dosage of each decoction prepared in each example was controlled to be 500 ml. The decoction prepared from the Chinese herbal compound for lowering blood uric acid and relieving pain described in Examples 1 to 5 was orally administered to different groups of rats, twice a day, with each rat taking 10 ml orally each time. The treatment course was 3 weeks, and the five groups of rats were all fed in the same way.

[0017] (4) Comparison of treatment effects was performed, as shown in Table 2.

[0018] Table 2 Comparison of therapeutic effects of Examples 1 to 5

[0019] Clinical trials: The applicant collected 80 patients diagnosed with hyperuricemia of dampness and stasis type from outpatients and inpatients of the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine in the early stage, including 52 males and 28 females; the youngest was 24 years old, the oldest was 65 years old, and the average age was 43 years old. They were divided into two groups using the random number table method: the treatment group included 28 males and 12 females, aged 30 to 65 years old; the course of disease was 2 months to 15 years; the control group included 24 males and 16 females, aged 24 to 65 years old; the course of disease was 1 month to 14 years. There was no statistical significance in the age, gender, and course of disease between the two groups of patients, and they were comparable.

[0020] The treatment group took the medicine prepared by the Chinese medicine compound for lowering blood uric acid and relieving pain recorded in Example 3 orally twice a day, with a total daily dosage of one dose (i.e., the amount in Table 1 above); the control group took allopurinol (produced by Beijing Yanjing Pharmaceutical Co., Ltd.) twice a day, 100 mg each time orally (the above is the daily routine dose for treatment); the treatment course of the treatment group and the control group was 3 weeks. Both groups of patients adopted the same dietary lifestyle intervention method.

[0021] Comparison of clinical efficacy: 40 cases in the treatment group, 26 cases were clinically cured, 10 cases were markedly effective, 3 cases were effective, 1 case was ineffective, and the total effective rate was 97.5%; 40 cases in the control group, 9 cases were clinically cured, 8 cases were markedly effective, 12 cases were effective, 11 cases were ineffective, and the total effective rate was 72.5%. The difference in clinical efficacy between the two groups was statistically significant ( P < 0. 05).

[0022] The efficacy standard is formulated in accordance with the "Guidelines for Clinical Research of New Chinese Medicines". Clinical cure: Symptoms completely disappear and blood uric acid returns to normal; marked effect: main symptoms improve, blood uric acid drops significantly or approaches normal (decline ≥ 20%); effective: main symptoms are relieved, blood uric acid drops compared with before (decline 10% ≤<20%); ineffective: does not meet the effectiveness standard.

[0023] Comparison of serum uric acid (UA), serum creatinine (Cr), and blood urea nitrogen (BUN) levels in the two groups before and after treatment: see Table 3. The serum uric acid levels in the two groups after treatment were significantly lower than those before treatment, and the difference was statistically significant ( P <0. 01). There was no statistically significant difference in the serum uric acid levels between the two groups after treatment ( P > 0. 05). There was no statistically significant difference in serum creatinine and blood urea nitrogen before and after treatment ( P > 0. 05), there was no statistically significant difference between the two groups after treatment ( P > 0.5). There were no abnormalities in the liver and kidney function, routine stool and blood routine safety indicators in the control group and the treatment group. No adverse reactions occurred in the treatment group during the treatment. In the control group, 2 cases had mild stomach discomfort and 1 case had mild drug rash, but all continued to participate in the treatment.

[0024] Table 3. Comparison of UA, Cr, and BUN levels between the two groups before and after treatment (X±s)

[0025] Salt-coptis chinensis has a bitter and cold nature, and is especially good at clearing dampness and heat from the lower burner. Wine-coptis chinensis has a bitter and cold nature, and can clear heat and dampness, purge fire and detoxify. It is the main medicine together with salt-coptis chinensis.

[0026] Atractylodes lancea has a bitter, dry and slightly warm nature. It can strengthen the spleen and eliminate dampness, and treat both the symptoms and the root cause. It is a minister medicine.

[0027] They are used together. Salted Phellodendron and wine-flavored Coptis are good at clearing heat, while Atractylodes is good at drying dampness. They have unique effects, and the flavors of the herbs are balanced to make the herbs mild to prevent damage to the stomach qi. Coix seeds are sweet and mild in nature, and can penetrate dampness and remove numbness, strengthen the spleen and replenish the middle, discharge pus and eliminate carbuncle, relax muscles and relieve urgency, and clear heat and detoxify. Papaya is sour and warm in nature, and can relax muscles and activate collaterals, harmonize the stomach and eliminate dampness. Sichuan Cyathula is bitter and sweet in nature, and can promote blood circulation and menstruation, remove blood stasis and relieve pain, nourish the liver and kidneys, strengthen muscles and bones, promote diuresis, and guide blood downward. It is used as a guiding drug.

[0028] Fried Evodia rutaecarpa is bitter, pungent and hot, which can dispel cold and relieve pain, reduce adverse reactions and stop vomiting, and can counteract the bitter and cold stomach-damaging nature of salt coptis chinensis. Raw Astragalus membranaceus is sweet and slightly warm in nature, which can replenish qi and strengthen the spleen, promote diuresis and reduce swelling. If the spleen is healthy, dampness can be eliminated. Raw Astragalus membranaceus and Coix seeds can enhance the effect of strengthening the spleen and removing dampness. Earthworms are salty and cold in nature, and are good at clearing heat, calming the liver, and unblocking meridians. The Compendium of Materia Medica points out that "its nature is cold and descends, so it can relieve various heat diseases, descends so it can promote urination, treat foot diseases and dredge meridians." It is cold in nature so it can treat heat diseases, and it tends to descend so it can promote urination, treat foot diseases, and dredge meridians. Earthworms, combined with salt coptis chinensis and wine coptis chinensis, can enhance the effect of clearing heat. When combined with Sichuan cyathulae, it tends to go straight down, which can make it easier for drugs to reach the diseased feet. Corydalis yanhusuo is pungent, bitter and warm in nature, and has the effects of promoting qi and blood circulation and relieving pain.

[0029] Phellodendron contains a variety of chemical components, mainly including alkaloids, phenolic acids, limonoids, sterols, volatile oils, etc. Alkaloid components are considered to be the main chemical components of salt Phellodendron, accounting for about 4.82%. Including protoberberine alkaloids such as berberine, phellodendronine, dihydroberberine jatrorrhizine, etc.; aporphenic alkaloids such as magnolia alkaloids, oxidized magnolia alkaloids, etc.; benzoisoquinoline alkaloids such as isoliensinine, etc. Berberine is the main metabolite of berberine, the active ingredient of Phellodendron, with higher bioavailability and higher plasma concentration. Both raw and salt products of Phellodendron can reduce the activity of xanthine oxidase in the liver, resulting in a decrease in the serum uric acid level in rats with hyperuricemia. Phellodendron water extract can inhibit the increase of xanthine oxidase activity and its mRNA and protein expression in the liver of mice caused by potassium oxonic acid, thereby inhibiting the synthesis of blood uric acid and reducing the level of blood uric acid. Berberine-7 is the best compound for anti-inflammatory and analgesic effects, while berberine-17 can significantly reduce the serum uric acid level in hyperuricemia model mice. Phellodendron chinense raw products, different processed products of Phellodendron chinense, and Phellodendron chinense alcohol extract can effectively reduce the UA and Cr levels in hyperuricemia model rats, and inhibit the joint swelling of GA rats, exerting an anti-gout effect. Phellodendron chinense water extract mainly inhibits UA production by reducing the serum and liver UA and Cr levels of hyperuricemia mice and downregulating the expression levels of URAT1 mRNA and protein in the kidneys, thereby exerting a uric acid-lowering effect. Berberine and dihydroberberine reduce the blood uric acid level by downregulating the expression of URAT1 and GLUT9 in the kidneys of hyperuricemia mice. At the same time, berberine can also downregulate the level of CRP inflammatory factors and increase the activity of superoxide dismutase (SOD) to alleviate oxidative stress and inflammatory damage in hyperuricemia mice. Berberine can downregulate the expression of GLUT9 and URAT1 in the kidney tissue of hyperuricemia mice, and upregulate the protein and mRNA levels of OAT1 / 3 and ABCG2 to play a role in treating hyperuricemia. Phellodendron chinense and its chemical components can inhibit the expression and generation of inflammatory mediators by inhibiting the TLR / MyD88 / NF-κB, MAPK, JAK2 / STAT3 signaling pathways, activating the Nrf2 / HO-1 antioxidant signaling pathway, thereby reducing inflammatory damage to affected joints and kidneys and delaying the development of gout.

[0030] As a traditional antibacterial drug, Rhizoma Coptidis can relieve symptoms such as redness, swelling, heat and pain in joints. Its main ingredient, berberine, has multiple pharmacological effects such as inhibiting inflammatory response, anti-oxidation, anti-endothelial damage, and anti-tumor. Experiments have shown that berberine can target NIMA-associated protein kinase 7 (NEK7), specifically block the interaction between NEK7-NLRP3, and fight inflammation by continuously inhibiting the release of IL-1β. Berberine can also inhibit the activation of NLRP3 inflammasomes, thereby reducing inflammation induced by urate crystals in rats and cell models. Experimental studies have shown that berberine can regulate the expression of mRNA, NLRP3 and IL-1 proteins through the NLRP3 inflammasome complex, and ultimately lead to a decrease in the activity of the catalytic domain (p20) of Caspase-1, inhibiting the levels of IL-1β and IL-18, and alleviating inflammation caused by urate crystals.

[0031] There are many active ingredients in Atractylodes lancea, and the following are the main ones found so far: enyne components (such as atractylodesin), sesquiterpenoid components (such as β-eudesmol, cypermethrin, atractylodes lactone), sugars, sterols, etc. The main component of Atractylodes lancea - Atractylodes polysaccharide can regulate the body's immunity by reducing the activity of caspase-3 and the expression of mitochondrial serine protease (Omi / HtrA2). In addition, the polysaccharide extract of Atractylodes lancea can improve the body's immunity by increasing the thymus index and spleen index, and can significantly affect the distribution of cell cycle and enhance cellular immune function. The volatile oils of raw Atractylodes lancea and bran-fried products can increase the level of anti-inflammatory factor IL-4, reduce the levels of pro-inflammatory factors TNF-α, IL-1β, IL-6, and IL-6, IL-8, TNF-α mRNA and protein expression, thereby reducing the inflammation level of gout patients. Atractylodes alcohol and β-eudesmol contained in Atractylodes lancea have the effect of inhibiting the central nervous system. Atractylodes alcohol and β-cineole have strong analgesic effects and can effectively improve the pain symptoms in the acute stage of gout.

[0032] Cyathulae contains a variety of chemical components, among which steroids, triterpenoid saponins and polysaccharides are high in content. In addition, it also contains chemical components such as polypeptides, alkaloids, volatile oils and flavonoids. The extract of Cyathulae root has a strong anti-inflammatory and detumescent effect. Its mechanism is that Cyathulae can improve the body's immune function, activate the phagocytic ability of mouse MΦ to bacteria, dilate blood vessels, improve circulation, and promote the absorption of inflammatory lesions. It also has a significant inhibitory effect on the swelling of the mouse foot inflammatory model caused by carrageenan. Cyathulae is more effective than Achyranthes bidentata in terms of egg white-induced foot swelling and inflammation in rats. Cyathulae polysaccharides are mainly composed of glucose, mannose, rhamnose, galacturonic acid, arabinose, etc., and have the advantages of easy absorption, low toxicity and good solubility. Its hypoglycemic, anti-tumor and immune regulation effects are particularly prominent. The alkaloids and volatile oil components in Rhizoma Cynoglossi are the main sources of "bitterness". The alkaloid components mainly include berberine, coptisine, epiberberine, etc. The volatile oil components mainly include long-chain alkanes, aldehydes, esters, alcohols, ketones, long-chain saturated fatty acids and pyrazine compounds. Rhizoma Cynoglossi can significantly inhibit the activity of MCF-7 cells and reduce the production of NO and tumor necrosis factor-α in macrophages induced by lipopolysaccharides (LPS), thereby triggering anti-inflammatory effects. β-Ecdysterone in Rhizoma Cynoglossi can inhibit the conduction of NF-κB signals, reduce the activity of cysteine ​​protease-3, downregulate the expression of matrix metalloproteinase 3 (MMP3), MMP9 and cyclooxygenase-2, and achieve anti-inflammatory and matrix degradation effects. Rhizoma Cynoglossi total saponins, geniposide and total steroids acted on mouse ear swelling, rat foot swelling and granuloma inflammation models, and the inflammatory response was alleviated. At present, the anti-inflammatory and analgesic mechanisms of Achyranthes bidentata mainly focus on improving the body's immune function and activating the phagocytic function of macrophages.

[0033] The main active ingredients of coix seed are esters, unsaturated fatty acids, sugars and lactams. The main pharmacological effects of coix seed polysaccharide are anti-oxidation, enhancing immunity, lowering blood sugar, regulating metabolism, etc. Coix seed polysaccharide can achieve anti-oxidative stress by directly eliminating free radicals in the body; it can promote T lymphocyte proliferation by activating the IL-2-mediated JAK3 / STAT5 signaling pathway, thereby improving cellular immune function and promoting immune regulation. Coix seed protein can effectively relieve foot swelling and pain in rats with adjuvant arthritis, and its mechanism may be related to reducing the mass concentration of TNF-α, IL-1, and IL-6 in serum. Glutamic acid will generate γ-aminobutyric acid after decarboxylation. It is a free non-protein amino acid and has a variety of functions in regulating body functions, such as regulating hormone metabolism, calming the nerves, and lowering blood pressure. The crude coix seed glycoprotein has strong reducing ability, can effectively inhibit Fe2+-induced lipid peroxidation, and has a certain scavenging ability for DPPH free radicals, hydroxyl free radicals and superoxide anions. There are many studies on flavonoids in coix shell and bran, including quercetin, apigenin, luteolin, tangeretin, naringin, rutin, etc. There are fewer monomeric flavonoid components identified in coix seed, mainly quercetin, kaempferol and rutin. Coix seed flavonoids have strong antioxidant and anti-inflammatory effects, have a good scavenging effect on DPPH free radicals, protect mitochondria from free radical damage, and the antioxidant capacity is in a dose-response relationship with the mass concentration of total flavonoids.

[0034] The chemical components of papaya mainly include triterpenes, phenylpropanoids, flavonoids and their glycosides, organic acids and their glycosides and esters, amino acids, oils, steroids and their glycosides, etc. The pharmacological effects of papaya mainly include analgesia, anti-inflammatory, and immune enhancement. Intragastric administration of papaya triterpenes can significantly inhibit the swelling of the paws of rats with adjuvant arthritis (AA), reduce the scores of polyarthritis, and improve the pathological changes of synovial tissue, reduce the pathological scores of synovial tissue, reduce the content of tumor necrosis factor-α, interleukin-1β, IL-6 and IL-8 in synovial tissue, and reduce the expression levels of p-serine threonine kinase and NF-kB p65 protein. Inhibiting the activation of Akt / NF-κB signaling pathway and the production of pro-inflammatory factors may be one of the important mechanisms for its anti-inflammatory effect. Papaya triterpenes can also exert anti-inflammatory effects by inhibiting the secretion of pro-inflammatory factors such as inducible nitric oxide synthase (iNOS), TNF-α, IL-1β and IL-6 by RAW264.7 cells (mouse mononuclear macrophage leukemia cells), and promoting the secretion of IL-4 and IL-10. Its mechanism of action is related to inhibiting the phosphorylation of nuclear factor κB-α and NF-κB p65, thereby restoring the balance between pro-inflammatory and anti-inflammatory factors.

[0035] Evodia rutaecarpa alkaloids are the main active ingredients of Evodia rutaecarpa, which are divided into indole alkaloids, quinolone alkaloids and other alkaloids according to their structures. Among the Evodia rutaecarpa alkaloids, evodiamine, dehydroevodiamine and dihydroevodiamine carpine have strong anti-inflammatory effects and are quality markers of the anti-inflammatory activity of Evodia rutaecarpa. Evodiamine, evodiamine and dehydroevodiamine can significantly downregulate proinflammatory cytokines and inflammatory mediators, and exert intestinal anti-inflammatory effects in the co-culture model of human colon adenocarcinoma cells and RAW264.7 macrophages.

[0036] Raw Astragalus has anti-infection and immunomodulatory effects. Astragaloside IV is the main active substance in raw Astragalus that plays an anti-infection and immunomodulatory role. TLRs are transmembrane pattern recognition receptors expressed by innate immune cells, which can trigger innate immune responses by controlling different signal transduction pathways. Among them, TLR4 recognizes the MSU crystal signal deposited in the joints and activates the activation of the downstream signal molecule MyD88, which can stimulate the transcription of the IL-1β gene and express the IL-1β precursor without any biological activity. In addition, MSU crystals interact with resident macrophages to form and activate NLRP3, the adapter protein ASC, and the cysteine ​​protease caspase-1 to assemble into the NLRP3 inflammasome, leading to the cleavage and activation of caspase-1. The activated capase-1 cleaves the IL-1β precursor and promotes the maturation and release of IL-1β. Cell autophagy is a state of balance in the body. The maintenance of inflammatory balance enables it to play an active role in innate immunity and adaptive immunity. Astragaloside IV regulates the immune inflammation of acute GA by inhibiting cell autophagy and regulating the activation of NLRP3 inflammasome.

[0037] Earthworms mainly contain chemical components such as amino acids, peptides, nucleosides and fatty acids. Earthworms can enhance the body's immune function, shorten the patient's inflammation time, accelerate wound healing, and thus improve the activation of phagocytes in the body. Earthworms can also inhibit the activity of macrophages by reducing harmful substances, improve the function of phagocytes, and enhance the body's ability to resist diseases. Studies have found that earthworm peptides can improve the phagocytic function of macrophages and enhance the body's immune protection.

[0038] Tetrahydropalmatine (THP) is the main active ingredient of the traditional Chinese medicine Corydalis. Studies have shown that THP can inhibit the formation of osteoclasts by inhibiting the nuclear factor activated B cell kappa light chain enhancer and mitogen-activated protein kinase pathways, and can reduce the accumulation of intracellular reactive oxygen species under the stimulation of irradiation. Studies have shown that THP has antioxidant activity, can inhibit the production of interleukin 1β (IL-1β) and nitric oxide induced by lipopolysaccharide, and reduce the activation of the nuclear factor activated B cell kappa light chain enhancer pathway induced by lipopolysaccharide. THP can inhibit the inflammatory response of acute gouty arthritis by reducing the production of proinflammatory cytokine IL-1β and the infiltration of neutrophils.

[0039] In summary, the ingredients in this Chinese medicine compound have the effects of lowering blood uric acid, anti-inflammatory and analgesic. The compatibility of various drugs and the complementary advantages of each ingredient increase the effects of lowering blood uric acid, anti-inflammatory and analgesic. The reasonable compatibility of each ingredient achieves a synergistic effect.

[0040] It should be noted that: The above-mentioned embodiments only express the specific implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present application. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the technical solution concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. A Chinese medicinal compound for lowering blood uric acid and relieving pain, It is characterized in that The drug is composed of the following raw materials by weight: 10-30 parts of Phellodendron chinense, 5-20 parts of Coptis chinensis, 15-30 parts of Atractylodes macrocephala, 20-40 parts of Coix seeds, 10-30 parts of Cyathula capitata, 10-30 parts of Papaya, 3-5 parts of stir-fried Evodia rutaecarpa, 15-50 parts of Astragalus membranaceus, 5-15 parts of Pheretima, and 20-30 parts of Corydalis yanhusuo.

2. A Chinese medicinal compound for lowering blood uric acid and relieving pain according to claim 1, It is characterized in that The cork is salt cork.

3. A Chinese medicinal compound for lowering blood uric acid and relieving pain according to claim 1, It is characterized in that The coptis root is prepared by brewing wine.

4. A Chinese medicinal compound for lowering blood uric acid and relieving pain according to claim 1, It is characterized in that The papaya is raw papaya slices.

5. A Chinese medicinal compound for lowering blood uric acid and relieving pain according to claim 1, It is characterized in that The astragalus root is raw.

6. A Chinese medicine preparation for lowering blood uric acid and relieving pain, It is characterized in that The preparation is prepared by the Chinese medicinal compound according to claims 1 to 5, including decoctions, pills, powders and pastes.

7. A method for preparing a Chinese medicine preparation for lowering blood uric acid and relieving pain, It is characterized in that The method for preparing the decoction according to claim 6 comprises the following steps: Step 1: Mix salted Phellodendron, wine-soaked Coptis, Atractylodes, Coix seeds, Cyathula, papaya, stir-fried Evodia rutaecarpa, raw Astragalus, earthworm, and Corydalis according to weight, and soak in cold water for 30 to 60 minutes; Step 2: Boil the mixed medicine obtained after soaking in step 1, and keep boiling for 20 to 30 minutes; Step 3: filtering the mixture obtained in step 2 to obtain the first medicinal solution and the first medicinal residue; Step 4: Add cold water to the first batch of medicinal residues, bring to a boil and keep boiling for 20 to 30 minutes; Step 5: Filter the mixture obtained in step 4 to obtain a second medicinal solution and a second medicinal residue; Step 6: Mix the first solution with the second solution to obtain a dose of medicine.

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