Traditional Chinese medicine compound composition taking glossy privet fruit as monarch drug as well as preparation method and application of traditional Chinese medicine compound composition

Abstract

The invention provides a traditional Chinese medicine compound composition taking glossy privet fruit as a monarch drug as well as a preparation method and application of the traditional Chinese medicine compound composition. The traditional Chinese medicine compound composition comprises the following raw materials in parts by weight: 15-35 parts of glossy privet fruit, 20-40 parts of oyster, 10-25 parts of curcuma zedoary, 5-25 parts of wine-treated rhubarb, 5-25 parts of roasted rhizoma atractylodis macrocephalae and 20-40 parts of oldenlandia diffusa. The traditional Chinese medicine compound composition is obvious in tumor treatment effect, can nourish liver and kidney, strengthen healthy energy to assist in removing food retention, promote qi circulation, break blood, remove food retention and relieve pain, has the effects of nourishing yin, softening hard mass, activating blood and detoxifying, and has the characteristics of high practical operability, high compliance, high tumor inhibition rate and low adverse reaction.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine compound composition with Ligustrum lucidum as a main drug, and a preparation method and application thereof. Background Art

[0002] Malignant tumors have become a serious threat to human health today. Liver malignant tumors are referred to as liver cancer. As one of the most common types of malignant tumors, it is easy to recur and metastasize. There are usually no symptoms or atypical symptoms in the early stage. It has the characteristics of hidden onset. Most patients have significant clinical symptoms, and most of them have entered the middle and late stages when diagnosed. The main methods of Western medicine for the treatment of liver cancer include surgical treatment, vascular interventional treatment, radiotherapy, local ablation treatment, molecular targeted therapy, etc. Among them, surgical treatment is considered to be an active and effective treatment option. However, many liver cancer patients have already spread and metastasized when they are diagnosed, and they are in the middle and late stages of liver cancer and lose the opportunity for surgery; although some patients undergo surgery and other treatment methods for auxiliary treatment, the five-year survival rate of patients who undergo surgery is only about 17%, and the metastasis and recurrence rate remains high, affecting the long-term survival of patients and becoming a bottleneck for further improving the overall efficacy of liver cancer.

[0003] With the continuous development of traditional Chinese medicine, its status in tumor prevention and treatment is increasing. A large number of clinical practices have shown that traditional Chinese medicine has unique advantages in reducing the toxic side effects of chemotherapy drugs, improving immunity, improving the quality of life of patients, and prolonging the survival of patients. Traditional Chinese medicine can improve the symptoms of patients with advanced liver cancer, improve immunity and their quality of life, so actively developing anti-tumor traditional Chinese medicine compound compositions will have important practical significance.

[0004] There are also some Chinese medicine formulas for anti-tumor in the prior art, but they all have more or less disadvantages, especially unclear efficacy, poor therapeutic effect, bitter taste, large side effects, and cannot be taken for a long time.

[0005] Therefore, there is an urgent need to develop a Chinese medicine compound composition that has obvious tumor treatment effects, nourishes the liver and kidneys, strengthens the body, has low adverse reactions, and is suitable for long-term use. Summary of the invention

[0006] The present invention aims at the above-mentioned problems existing in the prior art, and provides a Chinese medicine compound composition with Ligustrum lucidum fruit as the main drug. The Chinese medicine compound composition of the present invention has obvious effect in treating tumors, can nourish the liver and kidney, strengthen the body to help eliminate accumulation, and can promote qi and break blood, eliminate accumulation and relieve pain. The whole formula plays the effects of nourishing yin, softening hard masses, promoting blood circulation and detoxifying, and the medicine tastes sweet, is safe and non-toxic, and is suitable for long-term use.

[0007] The first aspect of the present invention provides a traditional Chinese medicine compound composition with Ligustrum lucidum as the main drug, wherein the traditional Chinese medicine compound composition comprises the following raw materials in parts by weight: 15-35 parts of Ligustrum lucidum, 20-40 parts of oysters, 10-25 parts of Curcuma zedoaria, 5-25 parts of wine-cooked rhubarb, 5-25 parts of stir-fried Atractylodes macrocephala, and 20-40 parts of Hedyotis diffusa.

[0008] Preferably, the Chinese medicine compound composition of the present invention comprises the following raw materials in parts by weight: 20-30 parts of Ligustrum lucidum fruit, 25-35 parts of oyster, 10-20 parts of Curcuma zedoaria, 10-20 parts of wine rhubarb, 10-20 parts of stir-fried Atractylodes macrocephala, and 25-35 parts of Hedyotis diffusa.

[0009] Preferably, the Chinese medicine compound composition of the present invention comprises the following raw materials in parts by weight: 22-28 parts of Ligustrum lucidum fruit, 27-33 parts of oyster, 12-18 parts of Curcuma zedoaria, 12-18 parts of wine rhubarb, 12-18 parts of stir-fried Atractylodes macrocephala, and 27-33 parts of Hedyotis diffusa.

[0010] Preferably, the Chinese medicine compound composition of the present invention comprises the following raw materials in parts by weight: 25 parts of Ligustrum lucidum fruit, 30 parts of oyster, 15 parts of Curcuma zedoaria, 15 parts of wine rhubarb, 15 parts of stir-fried Atractylodes macrocephala, and 30 parts of Hedyotis diffusa.

[0011] The second aspect of the present invention provides a method for preparing the above-mentioned Chinese medicine compound composition, comprising the following steps:

[0012] Step 1: Take the weight portions of Ligustrum lucidum, oyster, Curcuma zedoaria, wine rhubarb, stir-fried Atractylodes macrocephala and Hedyotis diffusa of the above-mentioned Chinese medicine compound composition, add water with a weight of 10-15 times the total weight of the raw materials to cover the drugs, and boil for one hour;

[0013] Step 2: extracting a first clear medicinal juice from the boiled product obtained in step 1, and obtaining a first solid medicinal residue;

[0014] Step 3: Add 8-10 times the weight of water to the first solid medicinal residue and boil for half an hour;

[0015] Step 4: extracting a second clear medicinal juice from the boiled product obtained in step 3, and obtaining a second solid medicinal residue;

[0016] Step 5: Repeat steps 3-4 to obtain the third clear medicine juice;

[0017] Step 6: The first clear medicinal juice, the second clear medicinal juice and the third clear medicinal juice are mixed, filtered to remove medicinal residues, concentrated, cooled to room temperature, and placed in a refrigerated environment for overnight to obtain a Chinese medicine compound preparation.

[0018] The third aspect of the present invention provides the use of the above-mentioned Chinese medicine compound composition in the preparation of anti-tumor drugs or medical materials.

[0019] The fourth aspect of the present invention provides the use of the above-mentioned Chinese medicine compound composition in the preparation of multi-target anti-tumor drugs or medical materials.

[0020] The Chinese medicine compound composition with Ligustrum lucidum as the main drug provided by the present invention and its preparation method and application can bring the following beneficial technical effects:

[0021] 1. The Chinese medicine compound composition of the present invention uses Ligustrum lucidum as the main medicine, has a sweet taste, is safe and non-toxic, can improve the body's immunity, protect liver function and resist tumors, and is more suitable for patients to take for a long time.

[0022] 2. From the perspective of the entire compound compatibility, the Chinese medicine compound composition of the present invention takes into account the functions of the liver, spleen, large intestine, small intestine and other internal organs, has multiple targets, good synergy and excellent clinical effect.

[0023] 3. The Chinese medicine compound composition of the present invention is a kind of reasonable proportion, definite curative effect, safe clinical application, which can not only nourish the liver and kidney, strengthen the body to help eliminate the accumulation, but also promote qi and blood circulation, eliminate the accumulation and relieve pain, and the whole formula plays the effect of nourishing yin, softening hardness, promoting blood circulation and detoxifying. Experiments show that the compound composition has a good growth inhibitory effect on the human liver cancer cell SMMC-7721 nude mouse transplant tumor model, and the tumor inhibition rate can reach 51.1%.

[0024] 4. The Chinese medicine compound composition of the present invention can be used to prepare anti-tumor drugs or medical materials, and can achieve the technical effects of practical operability, high compliance, high tumor inhibition rate, definite efficacy, clinical application safety and low adverse reactions in anti-tumor. DETAILED DESCRIPTION

[0025] The exemplary embodiments of the present invention are described in detail below. It should be noted that the relative configuration of the tables, digital representations and numerical values ​​described in these embodiments does not limit the scope of the present invention unless otherwise specifically stated. The experimental methods used in the embodiments of the present invention are conventional methods unless otherwise specified.

[0026] The raw materials of the Chinese medicine compound composition with Ligustrum lucidum as the main drug provided by the present invention include Ligustrum lucidum, oyster, Curcuma zedoaria, wine-cooked rhubarb, stir-fried Atractylodes macrocephala and Hedyotis diffusa, and the weight proportions of each raw material are: 15-35 parts of Ligustrum lucidum, 20-40 parts of oyster, 10-25 parts of Curcuma zedoaria, 5-25 parts of wine-cooked rhubarb, 5-25 parts of stir-fried Atractylodes macrocephala, and 20-40 parts of Hedyotis diffusa. The characteristics of the original drugs in the Chinese medicine compound composition of the present invention are as follows:

[0027] Ligustrum lucidum:

[0028] [Properties]: sweet and bitter in taste, cool in nature, enters the liver and kidney meridians.

[0029] [Effects]: It has the effects of nourishing the liver and kidneys, cooling blood and stopping bleeding, strengthening the body and helping to eliminate accumulation.

[0030] Curcuma zedoaria:

[0031] [Properties]: Spicy and bitter in taste, warm in nature, enters the liver and spleen meridians.

[0032] [Effects]: It has the functions of promoting qi and removing blood, eliminating accumulation and relieving pain.

[0033] Oyster:

[0034] [Properties]: Salty in taste, neutral in nature, enters the liver and kidney meridians.

[0035] [Effects]: It has the functions of nourishing Yin and suppressing Yang, softening and dispersing lumps.

[0036] Wine rhubarb:

[0037] [Properties]: bitter in taste, cold in nature, enters the spleen, stomach, large intestine, heart and liver meridians.

[0038] [Effects]: It has the functions of purging the lungs, attacking stagnation, promoting blood circulation, cooling blood and detoxifying.

[0039] Hedyotis diffusa:

[0040] [Properties]: A ministerial drug, slightly bitter, sweet, cold. Enters the stomach, large intestine, and small intestine meridians.

[0041] [Effects]: It has the functions of clearing away heat, detoxifying, promoting dampness and relieving stranguria.

[0042] Stir-fried Atractylodes macrocephala:

[0043] [Medicinal properties]: sweet, bitter, warm in nature,

[0044] [Effects]: It can replenish qi and strengthen the spleen, dry dampness and promote diuresis, consolidate the exterior and stop sweating, and strengthen the body to eliminate evil.

[0045] The Chinese medicine compound composition of the present invention uses Ligustrum lucidum fruit as the main drug, has a sweet taste, is safe and non-toxic, can improve the body's immunity, protect liver function to resist tumors, and is more suitable for patients to take for a long time. In addition, from the perspective of the entire compound compatibility, the Chinese medicine compound composition of the present invention takes into account the functions of the liver, spleen, large intestine, small intestine and other internal organs, has multiple action targets, good synergy, and good clinical effect.

[0046] The Chinese medicine compound composition of the present invention is described below with reference to specific examples.

[0047] Example 1

[0048] The Chinese medicine compound composition of this embodiment comprises: 25 parts of Ligustrum lucidum fruit, 30 parts of oyster, 15 parts of Curcuma zedoaria, 15 parts of wine rhubarb, 15 parts of stir-fried Atractylodes macrocephala, and 30 parts of Hedyotis diffusa.

[0049] Example 2

[0050] The Chinese medicine compound composition of this embodiment comprises: 15 parts of Ligustrum lucidum fruit, 40 parts of oyster, 10 parts of Curcuma zedoaria, 10 parts of wine rhubarb, 10 parts of stir-fried Atractylodes macrocephala, and 20 parts of Hedyotis diffusa.

[0051] Example 3

[0052] The Chinese medicine compound composition of this embodiment comprises: 20 parts of Ligustrum lucidum fruit, 25 parts of oyster, 20 parts of Curcuma zedoaria, 20 parts of wine rhubarb, 20 parts of stir-fried Atractylodes macrocephala, and 25 parts of Hedyotis diffusa.

[0053] Example 4

[0054] The Chinese medicine compound composition of this embodiment comprises: 30 parts of Ligustrum lucidum fruit, 35 parts of oyster, 12 parts of Curcuma zedoaria, 12 parts of wine rhubarb, 12 parts of stir-fried Atractylodes macrocephala, and 35 parts of Hedyotis diffusa.

[0055] Example 5

[0056] The Chinese medicine compound composition of this embodiment comprises: 35 parts of Ligustrum lucidum fruit, 20 parts of oyster, 25 parts of Curcuma zedoaria, 25 parts of wine rhubarb, 25 parts of stir-fried Atractylodes macrocephala, and 40 parts of Hedyotis diffusa.

[0057] Example 6

[0058] The Chinese medicine compound composition of this embodiment comprises: 32 parts of Ligustrum lucidum fruit, 27 parts of oyster, 16 parts of Curcuma zedoaria, 16 parts of wine rhubarb, 16 parts of stir-fried Atractylodes macrocephala, and 38 parts of Hedyotis diffusa.

[0059] Example 7

[0060] This embodiment describes the preparation method of the Chinese medicine compound composition of the present invention. The specific steps of the preparation method are as follows:

[0061] Step 1: Take a certain weight of Ligustrum lucidum fruit, oyster, Curcuma zedoaria, wine-cooked rhubarb, stir-fried Atractylodes macrocephala and Hedyotis diffusa, add a certain amount (e.g. 10-15 times) of water to cover the medicine, and boil for a certain time.

[0062] Specifically, the weight parts of each medicinal material in this step are weighed according to the formula of Example 1 of the present invention, that is, 25g of Ligustrum lucidum, 30g of oyster, 15g of Curcuma zedoaria, 15g of wine rhubarb, 15g of stir-fried Atractylodes macrocephala, 30g of Hedyotis diffusa, and then the weighed medicinal materials are placed in a decoction container, and 14 times the weight of water is added thereto, and decocted for one hour. Wherein, the 14 times the weight of water mentioned in this step is 14 times the total weight of all raw materials, namely, Ligustrum lucidum, oyster, Curcuma zedoaria, wine rhubarb, stir-fried Atractylodes macrocephala and Hedyotis diffusa. For example, 25g of Ligustrum lucidum, 30g of oyster, 15g of Curcuma zedoaria, 15g of wine rhubarb, 15g of stir-fried Atractylodes macrocephala, 30g of Hedyotis diffusa, and the sum of the weights of each raw material is 130g, so the weight of water added should be: 130g×14=1820g.

[0063] Step 2: taking out the first clear medicinal juice from the boiled product obtained in step 1, and obtaining the first solid medicinal residue.

[0064] Specifically, in this step, the boiled medicinal juice must be carefully separated as much as possible, and the first clear medicinal juice and the first solid medicinal residue are separated by filtering or other tools, and the first solid medicinal residue is placed in the decoction container.

[0065] Step 3: Add 8-10 times the weight of water to the first solid medicinal residue and boil for half an hour.

[0066] Specifically, the amount of water added in this step is also calculated according to the sum of the weights of all the Chinese medicinal materials in the raw materials. Taking the example in step 1 as an example, the sum of the weights of the raw materials is 130g. Then in this step, the weight of the water calculated according to 8 times the weight is 130g×8=1040g, and the weight of the water calculated according to 10 times the weight is 130g×10=1300g. In this way, the amount of water added in this step should be between 1040g and 1300g, that is, between 8 and 10 times the total weight of the raw materials.

[0067] Step 4: taking out a second clear medicinal juice from the boiled product obtained in step 3, and obtaining a second solid medicinal residue.

[0068] Specifically, as in step 2, in this step, the boiled medicinal juice must be carefully separated as much as possible. Filtering or other tools can be used to assist in separating the second clear medicinal juice from the second solid medicinal residue, and the second solid medicinal residue is placed in the decoction container.

[0069] Step 5: Repeat steps 3-4 to obtain the third clear medicine juice.

[0070] Specifically, this step is a repeated step of step 3 and step 4, except that the medicinal juice obtained by separating the boiled material is called the third clear medicinal juice.

[0071] Step 6: The first clear medicinal juice, the second clear medicinal juice and the third clear medicinal juice are mixed, filtered to remove the medicinal residue, concentrated, cooled to room temperature, and then placed in a refrigerated environment (e.g., -70°C to -90°C) for overnight refrigeration to obtain a Chinese medicine compound preparation.

[0072] Specifically, in this step, the mixed clear medicine juice can be filtered using any filtering tools such as a filter screen and a funnel, and concentrated using any common medicine juice concentration method, and then the medicine juice is cooled to room temperature and placed in a refrigerated environment, such as -80°C, overnight, such as a refrigerator, a cold storage, etc. Finally, it is taken out from the refrigerated environment to obtain the Chinese medicine compound composition preparation of the present invention.

[0073] The above describes the preparation process of the Chinese medicine compound composition of the present invention by taking the formula of the Chinese medicine compound composition of Example 1 as an example. The preparation methods of the Chinese medicine compound composition preparations of other embodiments are similar thereto and will not be described in detail here.

[0074] Experimental verification

[0075] The efficacy of the Chinese medicine compound composition of the present invention is verified by experiments using the Chinese medicine compound composition prepared in Example 7 as an example.

[0076] 1. Effect of serum containing Chinese herbal medicine compound on proliferation of human hepatoma cell line SMMC-7721

[0077] The dosage of experimental rats was calculated using a conversion formula based on the body surface area of ​​humans and rats. In this experiment, the conversion formula used a commonly used calculation formula in the prior art, and the dosage obtained by calculation was used as the experimental drug.

[0078] After the rats were adapted to feeding for 7 days, all the rats were divided into a blank control group and a compound experimental group. The administration was started by gavage, specifically including:

[0079] [Phase of obtaining serum containing Chinese herbal compound medicine]

[0080] Rats in the blank control group were given normal saline twice a day.

[0081] The rats in the compound experimental group were given a Chinese herbal compound preparation at a concentration of 46.8 g / kg daily, divided into two doses per day.

[0082] The specific dosage is as follows:

[0083] The first time, the blank control group was given normal saline, and the compound experimental group was given a Chinese herbal compound preparation with a concentration of 46.8 g / kg;

[0084] After 2 hours, the blank control group was given normal saline for the second time, and the compound experimental group was given the Chinese herbal compound preparation with a concentration of 46.8 g / kg.

[0085] The two groups of rats were gavaged continuously for 3 days. One hour after the above administration on the third day, the two groups of rats were anesthetized with ether, and blood was collected from the abdominal aorta of the two groups of rats under sterile conditions. The blood was left to stand for more than 2 hours, centrifuged at 1500r / min for 15 minutes, and then the serum was collected. The two groups of serum were mixed separately, inactivated in a water bath at 56 degrees Celsius for 30 minutes, and the blank control group serum and the Chinese medicine compound drug-containing serum were obtained, which were packaged and stored in a -20℃ refrigerator for later use.

[0086] It should be noted that the above two groups of serum are mixed separately, which means that the serum obtained from all blank control group rats is mixed to obtain the blank control group serum, and the serum obtained from all compound experimental group rats is mixed to obtain the Chinese medicine compound drug-containing serum, and the blank control group serum and the Chinese medicine compound drug-containing serum are processed separately and are not mixed with each other. The method of mixing the serum of each group of rats together can avoid errors caused by differences between animals.

[0087] [Experimental stage of human liver cancer cell SMMC-7721]

[0088] The Chinese medicine compound drug-containing serum obtained above was used to prepare a Chinese medicine compound drug-containing serum culture fluid. Specifically, a Chinese medicine compound drug-containing serum culture fluid containing a concentration of 2%, 4%, 6%, 8%, 10%, 12%, 14%, and 16% of the Chinese medicine compound drug-containing serum was prepared. The blank control group culture fluid prepared contained a blank control group serum concentration of 10%. The Chinese medicine compound drug-containing serum culture fluid containing a concentration of 2%, 4%, 6%, 8%, 10%, 12%, 14%, and 16% of the Chinese medicine compound drug-containing serum and the blank control group culture fluid were respectively acted on SMMC-7721 human liver cancer cells for 24 hours and 48 hours, and the experimental data obtained were listed in Table 1.

[0089] Table 1 shows the effect of the Chinese medicine compound medicated serum on the proliferation of SMMC-7721 cells. As shown in Table 1, the Chinese medicine compound medicated serum of the present invention has a significant inhibitory effect on human liver cancer cell SMMC-7721. As the concentration of the Chinese medicine compound medicated serum increases, the cell inhibition rate IR increases, and the cell inhibition rate under the condition of 48 hours of action is significantly greater than the cell inhibition rate under the condition of 24 hours of action. Therefore, the experimental data show that the inhibition rate of liver cancer cells is dose-dependent and time-dependent. Among them, IR in Table 1 represents the inhibition rate, and OD represents the absorbance.

[0090] In addition, IC was calculated based on the MTT assay. 50 (half inhibitory concentration), IC under 24-hour action 50 The serum concentration of the Chinese medicine compound is 17.60%, and the IC value is 48 hours. 50 The serum concentration of the Chinese herbal compound was 15.58%.

[0091] In addition, the OD values ​​of the compound groups at each concentration were statistically significant compared with the OD values ​​of the blank control group (P<0.05).

[0092] Table 1 Effects of Chinese herbal compound serum on SMMC-7721 cell proliferation

[0093]

[0094] Note: Compared with the blank control group:1) P<0.05, 2) P<0.01

[0095] 2. Tumor inhibition rate of Chinese herbal compound on tumor-bearing mice

[0096] First, a nude mouse model of SMMC-7721 liver cancer was prepared. Specifically, human liver cancer cells SMMC-7721 were routinely cultured, and 1×10 SMMC-7721 cells in the logarithmic growth phase were cultured. 7 The cells were inoculated in the axilla of nude mice to prepare tumor-bearing nude mice.

[0097] Then, according to the dose and type of drug to be administered, the tumor-bearing nude mice were divided into a model control group, a compound high-dose group, a compound medium-dose group, a compound low-dose group, and a positive control group for in vivo experiments. The tumor-bearing nude mice in the model control group will not be administered. The dosage of the nude mice was calculated according to the conversion formula, and the dosage of the Chinese medicine compound was 16.9 g kg -1 、33.8g·kg -1 and 67.6 g kg -1 The tumor-bearing nude mice were determined as the low-dose compound group, the medium-dose compound group and the high-dose compound group. The positive control group was given cyclophosphamide. The following is a detailed description of the administration of the tumor-bearing nude mice.

[0098] Specifically, the Chinese medicine compound preparation was administered to tumor-bearing nude mice 24 hours after inoculation of SMMC-7721 cells. -1 、33.8g·kg -1 and 67.6 g kg -1 The nude mice bearing tumors in the low-dose compound group, the medium-dose compound group, and the high-dose compound group were treated with the drug twice a day for 14 days. The nude mice bearing tumors in the positive control group were given cyclophosphamide 0.03 g kg -1 , intraperitoneal injection, once every other day, for 3 times in succession. Each group of nude mice was killed 24 hours after the last administration and the tumor was weighed to calculate the tumor inhibition rate. The experimental results are shown in Table 2.

[0099] Table 2 Tumor inhibition rate of Chinese herbal compound on tumor-bearing mice

[0100]

[0101] As shown in Table 2 above, the in vivo experimental results show that the high-dose compound group, the medium-dose compound group, and the low-dose compound group can all inhibit the growth of human liver cancer cells, and as the dose of the Chinese medicine compound increases, the tumor inhibition rate increases. The tumor inhibition rate of the high-dose Chinese medicine compound group can reach 51.1%, while the tumor inhibition rate of the positive control group is 50.5%, which is lower than that of the high-dose Chinese medicine compound group.

[0102] In summary, the experimental results show that the Chinese medicine compound composition provided by the present invention has a significant inhibitory effect on mouse transplanted tumors, and the tumor inhibition rate is dose-dependent.

[0103] 3. Detection of spleen index

[0104] After the nude mice were killed, the spleens were separated and weighed. The spleen index was calculated according to formula (1). The results are shown in Table 3.

[0105] Spleen index = spleen mass (mg) / body weight (10g) (1) Table 3 shows the effect of the Chinese herbal compound on the spleen index of nude mice

[0106]

[0107] As shown in Table 3, compared with the model control group, the spleen index of the positive control group decreased, while the spleen index of each compound group was significantly increased. The spleen is an immune organ of the body. This experiment proves that the Chinese medicine compound composition of the present invention enhances the immune function of tumor-bearing nude mice.

[0108] In summary, the Chinese medicine compound composition of the present invention is a Chinese medicine compound composition with reasonable proportion, definite efficacy, safe clinical application, and multi-target anti-tumor effect. It has low adverse reactions, can not only nourish the liver and kidney, strengthen the body to help eliminate accumulation, but also promote qi and blood circulation, eliminate accumulation and relieve pain, and the whole formula plays the effect of nourishing yin, softening hardness, and promoting blood circulation and detoxification. Experiments show that the compound composition has a good growth inhibitory effect on the human liver cancer cell SMMC-7721 nude mouse transplant tumor model, and the tumor inhibition rate can reach 51.1%. It can be seen that the Chinese medicine compound composition provided by the present invention has a good application prospect in anti-tumor.

[0109] Specifically, the Chinese medicine compound composition of the present invention can be used to prepare anti-tumor drugs or anti-tumor medical materials. In addition, the Chinese medicine compound composition of the present invention can be used to prepare multi-target / multi-target anti-tumor drugs or medical materials.

[0110] The anti-tumor drug or medical material prepared according to the Chinese medicine compound composition of the present invention can achieve the technical effects of practical operability, high compliance, high tumor inhibition rate, definite efficacy, safe clinical application and low adverse reactions in terms of anti-tumor.

[0111] Although the present invention has been described above with reference to exemplary embodiments, they are only preferred embodiments of the present invention and cannot be used to limit the protection scope of the present invention. Any modifications, equivalent substitutions and improvements made according to the spirit and essence of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese medicine compound composition with Ligustrum lucidum as the main drug, characterized in that: The Chinese medicine compound composition comprises the following raw materials in parts by weight: 15-35 parts of Ligustrum lucidum fruit, 20-40 parts of oyster, 10-25 parts of Curcuma zedoaria, 5-25 parts of wine rhubarb, 5-25 parts of stir-fried Atractylodes macrocephala, and 20-40 parts of Hedyotis diffusa.

2. The Chinese medicine compound composition according to claim 1, characterized in that: The Chinese medicine compound composition comprises the following raw materials in parts by weight: 20-30 parts of Ligustrum lucidum fruit, 25-35 parts of oyster, 10-20 parts of Curcuma zedoaria, 10-20 parts of wine rhubarb, 10-20 parts of stir-fried Atractylodes macrocephala, and 25-35 parts of Hedyotis diffusa.

3. The Chinese medicine compound composition according to claim 1, characterized in that: The Chinese medicine compound composition comprises the following raw materials in parts by weight: 22-28 parts of Ligustrum lucidum fruit, 27-33 parts of oyster, 12-18 parts of Curcuma zedoaria, 12-18 parts of wine rhubarb, 12-18 parts of stir-fried Atractylodes macrocephala, and 27-33 parts of Hedyotis diffusa.

4. The Chinese medicine compound composition according to claim 1, characterized in that: The Chinese medicine compound composition comprises the following raw materials in parts by weight: 25 parts of Ligustrum lucidum fruit, 30 parts of oyster, 15 parts of Curcuma zedoaria, 15 parts of wine rhubarb, 15 parts of stir-fried Atractylodes macrocephala, and 30 parts of Hedyotis diffusa.

5. The method for preparing the preparation of the Chinese medicine compound composition according to any one of claims 1 to 4, characterized in that: The following steps are involved: Step 1: Take the weight portions of Ligustrum lucidum, oyster, Curcuma zedoaria, wine rhubarb, stir-fried Atractylodes macrocephala and Hedyotis diffusa according to any one of claims 1 to 4, add water with a weight of 10 to 15 times the total weight of the raw materials to cover the drugs, and boil for one hour; Step 2: extracting a first clear medicinal juice from the boiled product obtained in step 1, and obtaining a first solid medicinal residue; Step 3: adding water with a weight of 8-10 times the total weight of the raw materials to the first solid medicinal residue and boiling for half an hour; Step 4: extracting a second clear medicinal juice from the boiled product obtained in step 3, and obtaining a second solid medicinal residue; Step 5: Repeat steps 3-4 to obtain the third clear medicine juice; Step 6: Mix the first clear medicinal juice, the second clear medicinal juice and the third clear medicinal juice, filter out the medicinal residue, concentrate, cool to room temperature, place in a refrigerated environment and refrigerate overnight to obtain a Chinese medicine compound preparation.

6. Use of the Chinese medicine compound composition according to any one of claims 1 to 4 in the preparation of anti-tumor drugs or medical materials.

7. Use of the Chinese medicine compound composition according to any one of claims 1 to 4 in the preparation of multi-target anti-tumor drugs or medical materials.

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