An integrated device and method for microalgae cultivation, harvesting and oil preparation

Abstract

The present invention relates to the technical field of microbiological devices, and in particular to an integrated device and method for microalgae cultivation, harvesting and oil preparation. Wastewater to be treated and an appropriate amount of microalgae cells are sequentially added from an injection port into a microalgae cultivation main chamber, and then the temperature, light, and stirring speed parameters are set according to predefined optimal culture conditions to start microalgae cultivation and wastewater treatment. The present invention realizes efficient and clean treatment of various types of wastewater, especially seaweed processing wastewater, and rapid harvesting of microalgae cells. The obtained biomass can be further used for the extraction of high-value active substances such as bio-oil and the development of microalgae biological products, thereby generating considerable economic benefits. In addition, by integrating the treatment of wastewater, the harvesting of highly economical microalgae, and the preparation of active substances, rapid separation of microalgae cells from culture fluid and efficient preparation of oil are realized, thereby effectively simplifying the harvesting process of large-scale microalgae cultivation and significantly reducing the harvesting cost.

Description

Technical Field

[0001] The present invention relates to the technical field of microbiological devices, and in particular to a device and method for integrating microalgae cultivation, harvesting and oil preparation. Background Art

[0002] With the rapid growth of the global economy, the acceleration of industrialization and urbanization, the large-scale discharge of various types of wastewater has caused serious impacts on the environment. At the same time, people's demand for clean energy and freshwater resources is also increasing. Building a green and efficient energy production system is an urgent problem to be solved. A large number of studies have shown that microalgae is a type of photoautotrophic organism with fast growth and strong adaptability. It has been widely used in the treatment of various wastewaters. Because it can quickly accumulate high-value compounds such as oils, fatty acids, and pigments, it has been considered a new generation of biomass resources, and has broad prospects for development and application in the fields of energy, food, and medicine. Wastewater treatment technology based on microalgae cultivation can effectively achieve carbon emission reduction and alleviate the freshwater resource crisis. At the same time, the obtained microalgae biomass can be used for the extraction of active substances such as oils and fats, as well as the preparation of algae biological products such as aquatic feed and biofertilizers.

[0003] Microalgae need to be under suitable temperature, light intensity and photoperiod conditions to grow better, accumulate more high-value compounds and achieve the best wastewater treatment effect. However, outdoor temperature and light conditions fluctuate greatly. Without human interference, it is difficult to achieve efficient cultivation of microalgae and efficient removal of wastewater. At the same time, it is difficult to quickly harvest the finished algae cells after large-scale cultivation of microalgae. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, the present invention provides an integrated device and method for microalgae cultivation, harvesting and oil preparation.

[0005] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0006] A device for integrating microalgae cultivation, harvesting and oil preparation comprises an upper transparent tempered glass shell and a lower transparent tempered glass shell, wherein a control panel for controlling the internal equipment of the upper transparent tempered glass shell is installed on the outside of the upper transparent tempered glass shell, a reagent port, a sampling port and a water inlet are provided on the outside of the upper transparent tempered glass shell, a microalgae cultivation main chamber is provided inside the upper transparent tempered glass shell, a plurality of lamps for illumination are fixedly inlaid on the inner wall of the microalgae cultivation main chamber, a sensor for detecting chemical oxygen demand, total nitrogen, total phosphorus, suspended particles and pH index is also fixedly installed inside the microalgae cultivation main chamber, a stirring paddle is installed inside the upper transparent tempered glass shell, A paddle is fixedly installed on the outside of the stirring paddle, and the shape of the paddle can be set to be square, round, arc, triangle, spiral or the like. A drainage pipe opening and a sample discharge pipe opening are fixedly installed on the upper and lower sides of the outside of the upper transparent tempered glass shell respectively. Filter membranes are arranged inside the drainage pipe opening and the sample discharge pipe opening. Both the drainage pipe opening and the sample discharge pipe opening are connected to the inside of the lower transparent tempered glass shell. A grease preparation chamber is opened inside the lower transparent tempered glass shell. A temperature control device for controlling the internal temperature of the microalgae breeding main chamber with the grease preparation chamber is arranged outside the lower transparent tempered glass shell. A crushing blade is rotatably connected inside the lower transparent tempered glass shell, and a motor is arranged outside the crushing blade for driving.

[0007] A switch assembly for connection is provided between the upper transparent tempered glass shell and the lower transparent tempered glass shell, and the switch assembly includes a lower support, a circular shaft and an upper support, the lower support is fixedly connected to the outside of the lower transparent tempered glass shell, the upper support is fixedly connected to the outside of the upper transparent tempered glass shell, the circular shaft is rotatably connected to the outside of the lower support and fixedly connected to the upper support, the upper transparent tempered glass shell rotates outside the upper transparent tempered glass shell with the circular shaft as the axis, and a regulating assembly for solid-liquid separation is provided above the inside of the grease preparation chamber.

[0008] The regulating component includes a solid-liquid separation partition for separating microalgae from wastewater, a circular hole valve, an outer ring and a plurality of connecting support plates. The circular hole valve is fixedly embedded in the center of the solid-liquid separation partition. The circular hole valve is used to control the connection of microalgae, wastewater and oil above the solid-liquid separation partition to the inside of the oil preparation chamber. The outer ring is fixedly connected to the outside of the solid-liquid separation partition. A plurality of connecting support plates are fixedly connected to the outside of the outer ring. One end of the connecting support plate away from the outer ring is fixedly connected to the inner wall of the oil preparation chamber. The solid-liquid separation partition is fixed above the inside of the oil preparation chamber through the outer ring and the connecting support plates. The solid-liquid separation partition is set to a funnel shape, and a baffle plate for shielding microalgae is set on the outside of the outer ring.

[0009] As a preferred technical solution of the present invention, a side slot is provided on the outside of the outer ring, and a side plate is movably connected inside the side slot. One end of the side plate away from the inside of the side slot is fixedly connected to the baffle plate. The baffle plate is driven by the side plate to slide upward or downward on the outside of the outer ring. When the baffle plate moves upward on the outside of the outer ring, it drives the side plate to move inside the side slot. The movement of the side plate inside the side slot prevents the baffle plate from moving out of the outside of the outer ring. A tooth groove is provided on the outside of the baffle plate.

[0010] As a preferred technical solution of the present invention, the switch assembly also includes a rubber gasket ring, a sealing base and a shielding ring for sealing the connection between the lower transparent tempered glass shell and the upper transparent tempered glass shell. The shielding ring is fixedly connected to the outside of the lower transparent tempered glass shell, the sealing base is fixedly connected to the outside of the lower transparent tempered glass shell and is located on the outside of the shielding ring. The end of the sealing base away from the lower transparent tempered glass shell is open-shaped, the rubber gasket ring is fixedly connected to the end of the upper transparent tempered glass shell close to the lower transparent tempered glass shell, the rubber gasket ring is movably embedded in the interior of the sealing base and connects the lower transparent tempered glass shell with the upper transparent tempered glass shell. The switch assembly also includes a fixed seat, a rotating ring and a lower mounting seat. The fixed seat is fixedly connected to the outside of the upper transparent tempered glass shell, the lower mounting seat is fixedly connected to the outside of the lower transparent tempered glass shell, the rotating ring is rotatably connected to the outside of the fixed seat, and a recess is fixedly connected below the outside of the lower mounting seat. The end of the rotating ring away from the fixed seat is rotatably embedded in the outside of the recess.

[0011] As a preferred technical solution of the present invention, a self-lifting component is arranged on the outside of the baffle plate, and the self-lifting component includes an inner notch, a sleeve roller and a rotating roller. The inner notch is opened inside the lower transparent tempered glass shell, and the rotating roller is rotatably connected to the inside of the inner notch. The sleeve roller is fixedly connected to the outside of the rotating roller. The sleeve roller is driven by the rotating roller to rotate inside the inner notch, and the outside of the sleeve roller is fixedly connected with a sleeve tooth, which is meshed with the tooth groove.

[0012] A circular groove is provided on the outside of the sleeve roller, and a torsion spring is fixedly connected to the inside of the circular groove. The torsion spring is movably sleeved on the outside of the rotating roller, and one end of the torsion spring away from the inside of the circular groove is fixedly connected to the inner wall of the inner groove. A wire groove is provided on the side of the sleeve roller away from the sleeve teeth, and a pull rope is fixedly connected to the inside of the wire groove. The pull rope is arranged in a winding shape inside the wire groove, and one end of the pull rope away from the wire groove movably passes through the inner groove and extends to be fixedly connected to the rubber gasket ring.

[0013] A method for using a device for integrating microalgae cultivation, harvesting and oil preparation comprises the following steps:

[0014] S1: Add the wastewater to be treated and an appropriate amount of microalgae cells into the microalgae cultivation main chamber from the injection port in sequence, and then set the parameters of temperature, light, and stirring speed according to the pre-defined optimal culture conditions to start microalgae cultivation and wastewater treatment;

[0015] S2: When the chemical oxygen demand, total nitrogen and total phosphorus concentrations of the wastewater are reduced to below 260, 15 and 0.5 mg / L respectively, flocculants are added according to the pre-defined optimal flocculation conditions, and the flocculation of algae cells and further removal of wastewater pollutants are carried out after setting the stirring speed and time;

[0016] S3: When the chemical oxygen demand, total nitrogen, total phosphorus and suspended particle concentrations of the wastewater are reduced to below 120, 10, 0.5 and 10 mg / L respectively, and the pH is within the range of 6-9, the supernatant after flocculation is discharged from the drainage pipe outlet on the lower side of the microalgae cultivation main chamber, and the flocculated algae cells are transferred from the circular hole valve in the middle of the solid-liquid separation partition to the oil preparation chamber, and the pollution index is detected at this time. If the pollution index does not reach the limit value, the flocculation step is repeated;

[0017] S4: Then, an oil extractant is added through the reagent port on the top of the device, and the oil extractant enters the oil preparation chamber through the circular hole valve. A temperature control device is arranged below the oil preparation chamber, and the temperature control range is 0-80°C, which is used to regulate the optimal temperature required for microalgae culture and cell drying. At the same time, the microalgae cells are broken by the crushing blade, and then extracted for a certain period of time, so that the obtained organic phase is discharged from the sample discharge port below the oil preparation chamber to obtain the crude oil extract, and the remaining algae residue is discharged from the sample discharge port together with the cleaned waste water.

[0018] As a preferred technical solution of the present invention, the oil preparation chamber is also used for the preliminary extraction of lutein, polysaccharides and protein active substances. The oil preparation method is to first add an oil extractant and then perform microalgae cell wall breaking treatment, followed by leaching. The obtained organic phase is the crude oil extract. The flocculant is prepared from one or more compounds of chitosan, sodium alginate, xanthan gum, starch and cellulose in appropriate proportions.

[0019] Compared with the prior art, the present invention can achieve the following beneficial effects:

[0020] 1. The present invention can achieve efficient and clean treatment of various types of wastewater, especially seaweed processing wastewater, and rapid harvesting of microalgae cells. The obtained biomass can be further used for the extraction of high-value active substances such as bio-oils and the development of microalgae biological products such as aquatic baits and bio-fertilizers, thereby generating considerable economic benefits. By integrating the treatment of wastewater, the harvesting of highly economical microalgae, and the preparation of active substances, the rapid separation of microalgae cells and culture fluid, and the efficient preparation of oils and fats can be effectively achieved, thereby effectively simplifying the harvesting process of large-scale cultivation of microalgae and significantly reducing the harvesting cost.

[0021] 2. The rotating ring is rotated to separate it from the recessed seat. At this time, the upper transparent tempered glass shell is rotated outside the lower support with the circular axis as the axis. The upper transparent tempered glass shell is rotated to separate from the lower transparent tempered glass shell to expose the solid-liquid separation partition to the outside. At this time, the microalgae and algae residue outside the solid-liquid separation partition can be quickly cleaned to avoid the situation where the microalgae and algae residue are not cleaned thoroughly.

[0022] 3. By setting a self-lifting component, when the upper transparent tempered glass shell drives the rubber gasket to rotate and detach from the inside of the sealing base, the rubber gasket drives the pull rope and pulls it inside the line slot. The pull rope is pulled out of the line slot by the tension and drives the sleeve roller to rotate the sleeve teeth with the roller as the center. The sleeve teeth are meshed with the tooth grooves and drive the baffle plate to move upward outside the outer ring when the upper transparent tempered glass shell is rotated to open, so as to shield the microalgae and algae residue outside the solid-liquid separation partition, thereby preventing the microalgae and algae residue outside the solid-liquid separation partition from leaking out when the upper transparent tempered glass shell is opened.

[0023] 4. The torsion spring arranged inside the circular notch shrinks the circular notch when the sleeve roller rotates. When the upper transparent tempered glass shell is closed again with the lower transparent tempered glass shell, the elastic force generated by the torsion spring drives the sleeve roller to rotate and rewinds the pull rope inside the line notch. When the sleeve roller rotates, it drives the sleeve teeth and the tooth grooves and resets the baffle plate downward, thereby achieving the effect of facilitating the reciprocating use of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 A three-dimensional surface diagram showing the interactive effects of flocculant concentration, mixing time and mixing speed on the removal rate of chemical oxygen demand in wastewater;

[0025] Figure 2 A three-dimensional surface diagram showing the interactive effects of flocculant concentration, mixing time and mixing speed on the flocculation efficiency of microalgae cells in the present invention;

[0026] Figure 3 This is the variance analysis table when the chemical oxygen demand removal rate of the present invention is the response value;

[0027] Figure 4 This is the variance analysis table when the flocculation efficiency of the present invention is the response value;

[0028] Figure 5 It is a schematic structural diagram of the transparent tempered glass housing of the present invention;

[0029] Figure 6 It is a structural schematic diagram of a transparent tempered glass housing according to the present invention;

[0030] Figure 7It is a schematic cross-sectional structure diagram of the transparent tempered glass housing of the present invention;

[0031] Figure 8 It is a schematic cross-sectional structure diagram of the transparent tempered glass housing according to the present invention;

[0032] Fig. 9 It is a schematic diagram of the structure of the stirring paddle of the present invention;

[0033] Fig.10 It is a structural schematic diagram of the roller sleeve of the present invention;

[0034] Fig.11 It is a structural schematic diagram of the solid-liquid separation partition of the present invention;

[0035] Fig.12 It is a structural schematic diagram of the lower mounting seat of the present invention.

[0036] Among them: 10, upper transparent tempered glass shell; 11, lower transparent tempered glass shell; 12, control panel; 13, lamp tube; 14, stirring paddle; 15, crushing blade; 16, microalgae cultivation main room; 17, oil preparation room; 18, sample discharge pipe outlet; 19, drainage pipe outlet; 20, solid-liquid separation partition; 21, round hole valve; 22, outer ring; 23, side notch; 24, side plate; 25, connection Support plate; 26, baffle plate; 27, tooth groove; 30, rubber gasket; 31, sealing base; 32, cover ring; 33, lower support; 34, round shaft; 35, upper support; 36, fixed seat; 37, rotating ring; 38, lower mounting seat; 39, recessed seat; 40, inner notch; 41, sleeve roller; 42, sleeve teeth; 43, torsion spring; 44, rotating roller; 45, circular ring notch; 46, wire notch; 47, pull rope. DETAILED DESCRIPTION

[0037] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work all belong to the protection scope of the present invention. The experimental methods in the following embodiments, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following embodiments, unless otherwise specified, can all be obtained from commercial channels.

[0038] Embodiment 1

[0039] An integrated device and method for microalgae cultivation, harvesting and oil preparation.

[0040] In order to solve the above technical problems, the present invention provides the following technical solutions: Figure 4-Figure 12A device for integrating microalgae cultivation, harvesting and oil preparation comprises an upper transparent tempered glass shell 10 and a lower transparent tempered glass shell 11. A control panel 12 for controlling the internal equipment of the upper transparent tempered glass shell 10 is installed on the outside of the upper transparent tempered glass shell 10. A reagent port, a sampling port and a water inlet are provided on the outside of the upper transparent tempered glass shell 10. A microalgae cultivation main chamber 16 is provided inside the upper transparent tempered glass shell 10. A plurality of lamps 13 for illumination are fixedly inlaid on the inner wall of the microalgae cultivation main chamber 16. A sensor for detecting chemical oxygen demand, total nitrogen, total phosphorus, suspended particles and pH index is also fixedly installed inside the microalgae cultivation main chamber 16. A stirring paddle 14 is installed inside the upper transparent tempered glass shell 10. A paddle is fixedly installed on the outside of 14, and the shape of the paddle can be set to be square, circular, arc, triangular and spiral shapes. A drainage pipe port 19 and a sample discharge pipe port 18 are fixedly installed on the upper and lower sides of the outside of the upper transparent tempered glass shell 10, respectively. The insides of the drainage pipe port 19 and the sample discharge pipe port 18 are both provided with filter membranes. The drainage pipe port 19 and the sample discharge pipe port 18 are both connected to the inside of the lower transparent tempered glass shell 11. A grease preparation chamber 17 is opened inside the lower transparent tempered glass shell 11. A temperature control device for controlling the internal temperature of the microalgae breeding main chamber 16 with the grease preparation chamber 17 is provided on the outside of the lower transparent tempered glass shell 11. A crushing blade 15 is rotatably connected to the inside of the lower transparent tempered glass shell 11, and a motor is provided on the outside of the crushing blade 15 for driving.

[0041] A switch assembly for connection is provided between the upper transparent tempered glass shell 10 and the lower transparent tempered glass shell 11, and the switch assembly includes a lower support 33, a circular shaft 34 and an upper support 35. The lower support 33 is fixedly connected to the outside of the lower transparent tempered glass shell 11, and the upper support 35 is fixedly connected to the outside of the upper transparent tempered glass shell 10. The circular shaft 34 is rotatably connected to the outside of the lower support 33 and is fixedly connected to the upper support 35. The upper transparent tempered glass shell 10 rotates outside the upper transparent tempered glass shell 10 with the circular shaft 34 as the axis. A regulating assembly for solid-liquid separation is provided above the inside of the grease preparation chamber 17.

[0042] The regulating component includes a solid-liquid separation partition 20 for separating microalgae from wastewater, a circular hole valve 21, an outer ring ring 22 and a plurality of connecting support plates 25. The circular hole valve 21 is fixedly embedded in the center of the solid-liquid separation partition 20. The circular hole valve 21 is used to control the connection of microalgae, wastewater and oil above the solid-liquid separation partition 20 to the inside of the oil preparation chamber 17. The circular hole valve 21 can completely separate or connect the microalgae breeding main chamber 16 and the oil preparation chamber 17. The outer ring ring 22 is fixedly connected to the outside of the solid-liquid separation partition 20, and a plurality of connecting support plates 25 are fixedly connected to the outside of the outer ring ring 22. One end of the connecting support plate 25 away from the outer ring ring 22 is fixedly connected to the inner wall of the oil preparation chamber 17. The solid-liquid separation partition 20 is fixed above the inside of the oil preparation chamber 17 through the outer ring ring 22 and the connecting support plate 25. The solid-liquid separation partition 20 is set in a funnel shape, and a baffle plate 26 for shielding microalgae is set on the outside of the outer ring ring 22.

[0043] A side slot 23 is provided on the outside of the outer ring 22, and a side plate 24 is movably connected inside the side slot 23. One end of the side plate 24 away from the inside of the side slot 23 is fixedly connected to a retaining ring plate 26. The retaining ring plate 26 is driven by the side plate 24 to slide upward or downward on the outside of the outer ring 22. When the retaining ring plate 26 moves upward on the outside of the outer ring 22, it drives the side plate 24 to move inside the side slot 23. The movement of the side plate 24 inside the side slot 23 prevents the retaining ring plate 26 from moving out of the outside of the outer ring 22. A tooth groove 27 is provided on the outside of the retaining ring plate 26.

[0044] The switch assembly also includes a rubber insert ring 30, a sealing base 31 and a shielding ring 32 for sealing the connection between the lower transparent tempered glass shell 11 and the upper transparent tempered glass shell 10. The shielding ring 32 is fixedly connected to the outside of the lower transparent tempered glass shell 11, and the sealing base 31 is fixedly connected to the outside of the lower transparent tempered glass shell 11 and is located on the outside of the shielding ring 32. The end of the sealing base 31 away from the lower transparent tempered glass shell 11 is open. The rubber insert ring 30 is fixedly connected to the end of the upper transparent tempered glass shell 10 close to the lower transparent tempered glass shell 11. The rubber insert ring 30 is movably embedded in the interior of the sealing base 31 and connects the lower transparent tempered glass shell 11 to the upper transparent tempered glass shell 10. The switch assembly also includes a fixed seat 36, a rotating ring 37 and a lower mounting seat 38. The fixed seat 36 is fixedly connected to the outside of the upper transparent tempered glass shell 10, the lower mounting seat 38 is fixedly connected to the outside of the lower transparent tempered glass shell 11, the rotating ring 37 is rotatably connected to the outside of the fixed seat 36, and a recessed seat 39 is fixedly connected below the outside of the lower mounting seat 38. The end of the rotating ring 37 away from the fixed seat 36 is rotatably embedded in the outside of the recessed seat 39. The rotating ring 37 is rotated to separate it from the recessed seat 39. At this time, the upper transparent tempered glass shell 10 is rotated outside the lower support 33 with the circular shaft 34 as the axis. The upper transparent tempered glass shell 10 is rotated to separate from the lower transparent tempered glass shell 11 to expose the solid-liquid separation partition 20 to the outside. At this time, the microalgae material and algae residue outside the solid-liquid separation partition 20 can be quickly cleaned to avoid the situation where the microalgae and algae residue are not thoroughly cleaned.

[0045] A self-lifting component is arranged on the outside of the retaining ring plate 26, and the self-lifting component includes an inner notch 40, a sleeve roller 41 and a rotating roller 44. The inner notch 40 is opened inside the lower transparent tempered glass shell 11, and the rotating roller 44 is rotatably connected to the inside of the inner notch 40. The sleeve roller 41 is fixedly connected to the outside of the rotating roller 44. The sleeve roller 41 is driven to rotate inside the inner notch 40 by the rotating roller 44. The outside of the sleeve roller 41 is fixedly connected with a sleeve tooth 42, and the sleeve tooth 42 is meshed with the tooth groove 27.

[0046] A circular notch 45 is provided on the outside of the sleeve roller 41, and a torsion spring 43 is fixedly connected inside the circular notch 45. The torsion spring 43 is movably sleeved on the outside of the rotating roller 44. One end of the torsion spring 43 away from the inside of the circular notch 45 is fixedly connected to the inner wall of the inner notch 40. A wire notch 46 is provided on the side of the sleeve roller 41 away from the sleeve teeth 42. A pull rope 47 is fixedly connected inside the wire notch 46. The pull rope 47 is arranged in a winding shape inside the wire notch 46. One end of the pull rope 47 away from the wire notch 46 movably passes through the inner notch 40 and extends to be fixedly connected to the plug-in rubber pad ring 30. By setting a self-lifting component, the upper transparent tempered glass shell 10 drives the plug-in rubber pad When the ring 30 rotates and disengages from the sealing base 31, the rubber pad ring 30 drives the pull rope 47 and pulls it inside the line slot 46. The pull rope 47 is pulled out of the line slot 46 and drives the sleeve roller 41 to make the sleeve teeth 42 rotate around the rotating roller 44. The sleeve teeth 42 engage with the tooth grooves 27 and drive the baffle plate 26 to move upward outside the outer ring 22 when the upper transparent tempered glass shell 10 is rotated to open, so as to block the microalgae and algae residue outside the solid-liquid separation partition 20, thereby preventing the microalgae and algae residue outside the solid-liquid separation partition 20 from leaking out when the upper transparent tempered glass shell 10 is opened.

[0047] A method for using a device for integrating microalgae cultivation, harvesting and oil preparation comprises the following steps:

[0048] S1: Add the wastewater to be treated and an appropriate amount of microalgae cells into the microalgae cultivation main chamber 16 from the injection port in sequence, and then set the parameters of temperature, light, and stirring speed according to the pre-defined optimal culture conditions to start microalgae cultivation and wastewater treatment;

[0049] S2: When the chemical oxygen demand, total nitrogen and total phosphorus concentrations of the wastewater are reduced to below 260, 15 and 0.5 mg / L respectively, flocculants are added according to the pre-defined optimal flocculation conditions, and the flocculation of algae cells and further removal of wastewater pollutants are carried out after setting the stirring speed and time;

[0050] S3: When the chemical oxygen demand, total nitrogen, total phosphorus and suspended particle concentrations of the wastewater are reduced to below 120, 10, 0.5 and 10 mg / L respectively, and the pH is within the range of 6-9, the supernatant after flocculation is discharged from the drainage port 19 at the lower side of the microalgae cultivation main chamber 16, and the flocculated algae cells are transferred from the circular hole valve 21 in the middle of the solid-liquid separation partition 20 to the oil preparation chamber 17, and the pollution index is detected at this time. If the pollution index does not reach the limit value, the flocculation step is repeated;

[0051] S4: Then, an oil extractant is added through the reagent port at the top of the device, and the oil extractant enters the oil preparation chamber 17 through the circular hole valve 21. A temperature control device is arranged below the oil preparation chamber 17, and the temperature control range is 0-80°C, which is used to regulate the optimal temperature required for microalgae culture and cell drying. The oil preparation chamber 17 is also used for the preliminary extraction of lutein, polysaccharides and protein active substances. The oil preparation method is to first add an oil extractant and then perform microalgae cell wall breaking treatment, followed by leaching, and the obtained organic phase is the crude oil extract. The flocculant is prepared from one or more compounds selected from chitosan, sodium alginate, xanthan gum, starch and cellulose in an appropriate proportion. At the same time, the microalgae cells are broken by the crushing blade 15, and then leached for a certain period of time, so that the obtained organic phase is discharged from the sample discharge pipe port 18 below the oil preparation chamber 17 to obtain the crude oil extract, and the remaining algae residue is discharged from the sample discharge pipe port 18 together with the cleaned wastewater.

[0052] Embodiment 2

[0053] Based on the first embodiment, Figure 1 , Figure 2 , Figure 3 and Figure 4 , taking the treatment of laver processing wastewater by using Scenedesmus obliquus as an example, the laver processing wastewater to be treated and Scenedesmus obliquus cells with a dry biomass of 0.12 g / L are added into the microalgae cultivation main chamber 16 from the injection port in sequence, and then the temperature, light intensity, light cycle and stirring speed of the device are set to 30°C, 4000 Lux, 24:0 h and 130 r / min respectively to start microalgae cultivation and wastewater treatment. During the cultivation of microalgae, appropriate amounts of samples are taken from the sampling port regularly to manually analyze the changes in indicators such as microalgae biomass and chemical oxygen demand of wastewater. Changes in indicators such as chemical oxygen demand, total nitrogen, total phosphorus, suspended particles, pH, etc. of wastewater can also be regularly recorded from the control panel 12.

[0054] When the chemical oxygen demand, total nitrogen and total phosphorus concentrations of the wastewater are reduced to below 260, 15 and 0.5 mg / L, respectively, a flocculant is added from the reagent port at the top of the upper transparent tempered glass shell 10 at a final concentration of 79.13 mg / L, the speed of the stirring paddle 14 is set to 162 r / min, and the stirring is stopped after 22 minutes of stirring, and the stirring is allowed to stand for 15 minutes or more, and the algae cells are completely precipitated to the bottom, and the chemical oxygen demand, total nitrogen, total phosphorus and suspended particle concentrations of the wastewater are reduced to below 120, 10, 0.5 and 10 mg / L, respectively, and the pH is within the range of 6-9, the flocculated supernatant is discharged from the drain pipe port 19 on the lower side of the microalgae cultivation main chamber 16, and the flocculated algae cells are transferred from the circular hole valve 21 in the middle of the solid-liquid separation partition 20 to the oil preparation chamber; if the pollution indicators do not reach the limit value, the flocculation step is repeated.

[0055] Then, add the oil extractant through the reagent port at the top of the upper transparent tempered glass shell 10 and the circular hole valve 21 in the middle of the solid-liquid separation partition 20, and at the same time open the crushing blade 15 to break the wall of the microalgae cells, and then extract for about 10 minutes, and the obtained organic phase is discharged from the sample discharge pipe port 18 on the lower side of the oil preparation chamber 17 to obtain the crude oil extract, and the remaining algae residue is discharged from the sample discharge pipe port 18 together with the cleaning waste water.

[0056] The chemical oxygen demand of laver processing wastewater treated according to the above steps can be reduced to 71.17 mg / L, and the flocculation efficiency of suspended particles can reach 99.02%. At the same time, 0.48 g / L of microalgae biomass and 18.63% of oil are obtained. The chemical oxygen demand, total nitrogen, total phosphorus and suspended particle concentration of the treated wastewater can effectively meet the emission requirements of the national "Pollutant Discharge Standard for Urban Wastewater Treatment Plants" (GB18919-2002).

[0057] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited thereto, and various changes can be made within the knowledge scope of technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. A device for integrating microalgae cultivation, harvesting and oil preparation, comprising an upper transparent tempered glass shell (10) and a lower transparent tempered glass shell (11), wherein a control panel (12) for controlling the internal equipment of the upper transparent tempered glass shell (10) is installed on the outside of the upper transparent tempered glass shell (10), and a reagent port, a sampling port and a water inlet are provided on the outside of the upper transparent tempered glass shell (10), characterized in that: The upper transparent tempered glass shell (10) is provided with a microalgae cultivation main chamber (16) inside, and a plurality of lamp tubes (13) for illumination are fixedly inlaid on the inner wall of the microalgae cultivation main chamber (16), and sensors for detecting chemical oxygen demand, total nitrogen, total phosphorus, suspended particles and pH index are also fixedly installed inside the microalgae cultivation main chamber (16), a stirring paddle (14) is installed inside the upper transparent tempered glass shell (10), and a paddle is fixedly installed outside the stirring paddle (14), and a drainage pipe opening (19) and a sample discharge pipe opening (18) are fixedly installed on the upper and lower sides of the outer part of the upper transparent tempered glass shell (10), respectively, and a fat preparation chamber (17) is provided inside the lower transparent tempered glass shell (11), and a temperature control device for controlling the internal temperature of the microalgae cultivation main chamber (16) covered with the fat preparation chamber (17) is arranged outside the lower transparent tempered glass shell (11), and a crushing blade (15) is rotatably connected inside the lower transparent tempered glass shell (11); A switch assembly for connection is provided between the upper transparent tempered glass shell (10) and the lower transparent tempered glass shell (11), the switch assembly comprising a lower support (33), a circular shaft (34) and an upper support (35); the lower support (33) is fixedly connected to the outside of the lower transparent tempered glass shell (11); the upper support (35) is fixedly connected to the outside of the upper transparent tempered glass shell (10); the circular shaft (34) is rotatably connected to the outside of the lower support (33) and is fixedly connected to the upper support (35); the upper transparent tempered glass shell (10) rotates outside the upper transparent tempered glass shell (10) with the circular shaft (34) as the axis; and a control assembly for solid-liquid separation is provided above the inside of the grease preparation chamber (17); The regulating component comprises a solid-liquid separation partition (20) for separating microalgae from wastewater, a circular hole valve (21), an outer ring (22) and a plurality of connecting support plates (25), wherein the circular hole valve (21) is fixedly embedded in the center of the solid-liquid separation partition (20), the outer ring (22) is fixedly connected to the outside of the solid-liquid separation partition (20), the plurality of connecting support plates (25) are fixedly connected to the outside of the outer ring (22), one end of the connecting support plate (25) away from the outer ring (22) is fixedly connected to the inner wall of the oil preparation chamber (17), the solid-liquid separation partition (20) is fixed above the inside of the oil preparation chamber (17) through the outer ring (22) and the connecting support plates (25), the solid-liquid separation partition (20) is set in a funnel shape, and a baffle plate (26) for shielding microalgae is arranged outside the outer ring (22).

2. The preparation device for the integrated method of microalgae cultivation, harvesting and oil preparation according to claim 1, characterized in that: The outer ring (22) is provided with a side notch (23) on the outside, and the inside of the side notch (23) is movably connected with a side plate (24). One end of the side plate (24) away from the inside of the side notch (23) is fixedly connected to a baffle plate (26). The baffle plate (26) is driven by the side plate (24) to slide upward or downward on the outside of the outer ring (22). A tooth groove (27) is provided on the outside of the baffle plate (26).

3. The preparation device for the integrated method of microalgae cultivation, harvesting and oil preparation according to claim 2, characterized in that: The switch assembly further comprises a rubber insert ring (30) for sealing the connection between the lower transparent tempered glass shell (11) and the upper transparent tempered glass shell (10), a sealing base (31) and a shielding ring (32); the shielding ring (32) is fixedly connected to the outside of the lower transparent tempered glass shell (11); the sealing base (31) is fixedly connected to the outside of the lower transparent tempered glass shell (11) and is located outside the shielding ring (32); one end of the sealing base (31) away from the lower transparent tempered glass shell (11) is open; the rubber insert ring (30) is fixedly connected to one end of the upper transparent tempered glass shell (10) close to the lower transparent tempered glass shell (11); the rubber insert ring (30) is movably embedded in the interior of the sealing base (31) and connects the lower transparent tempered glass shell (11) with the upper transparent tempered glass shell (10).

4. The preparation device for the integrated method of microalgae cultivation, harvesting and oil preparation according to claim 3, characterized in that: The switch assembly further comprises a fixed seat (36), a rotating ring (37) and a lower mounting seat (38); the fixed seat (36) is fixedly connected to the outside of the upper transparent tempered glass shell (10); the lower mounting seat (38) is fixedly connected to the outside of the lower transparent tempered glass shell (11); the rotating ring (37) is rotatably connected to the outside of the fixed seat (36); a recess (39) is fixedly connected below the outside of the lower mounting seat (38); and one end of the rotating ring (37) away from the fixed seat (36) is rotatably embedded in the outside of the recess (39).

5. The preparation device for the integrated method of microalgae cultivation, harvesting and oil preparation according to claim 4, characterized in that: A self-lifting assembly is arranged outside the baffle plate (26), and the self-lifting assembly includes an inner notch (40), a sleeve roller (41) and a rotating roller (44). The inner notch (40) is opened inside the lower transparent tempered glass shell (11), and the rotating roller (44) is rotatably connected inside the inner notch (40). The sleeve roller (41) is fixedly connected to the outside of the rotating roller (44). The sleeve roller (41) is driven by the rotating roller (44) to rotate inside the inner notch (40). The outside of the sleeve roller (41) is fixedly connected with a sleeve tooth (42), and the sleeve tooth (42) is meshedly connected with the tooth groove (27); A circular notch (45) is provided on the outside of the sleeve roller (41), a torsion spring (43) is fixedly connected to the inside of the circular notch (45), the torsion spring (43) is movably sleeved on the outside of the rotating roller (44), one end of the torsion spring (43) away from the inside of the circular notch (45) is fixedly connected to the inner wall of the inner notch (40), a wire notch (46) is provided on the side of the sleeve roller (41) away from the sleeve teeth (42), a pull rope (47) is fixedly connected to the inside of the wire notch (46), the pull rope (47) is arranged in a winding shape inside the wire notch (46), and one end of the pull rope (47) away from the wire notch (46) movably passes through the inner notch (40) and extends to be fixedly connected to the rubber insert ring (30).

6. The method for using the integrated device for microalgae cultivation, harvesting and oil preparation according to claim 1, characterized in that: The following steps are involved: S1: adding the wastewater to be treated and an appropriate amount of microalgae cells into the microalgae cultivation main chamber (16) from the injection port in sequence, and then setting the parameters of temperature, light, and stirring speed according to the pre-defined optimal culture conditions to start microalgae cultivation and wastewater treatment; S2: When the chemical oxygen demand, total nitrogen and total phosphorus concentrations of the wastewater are reduced to below 260, 15 and 0.5 mg / L respectively, flocculants are added according to the pre-defined optimal flocculation conditions, and the flocculation of algae cells and further removal of wastewater pollutants are carried out after setting the stirring speed and time; S3: When the chemical oxygen demand, total nitrogen, total phosphorus and suspended particle concentrations of the wastewater are reduced to below 120, 10, 0.5 and 10 mg / L respectively, and the pH is within the range of 6-9, the flocculated supernatant is discharged from the drainage pipe port (19) at the lower side of the microalgae cultivation main chamber (16), and the flocculated algae cells are transferred from the circular hole valve (21) in the middle of the solid-liquid separation partition (20) to the oil preparation chamber (17), and the pollution index is detected at this time. If the pollution index does not reach the limit value, the flocculation step is repeated; S4: Then, an oil extractant is added through the reagent port at the top of the device, and the oil extractant enters the oil preparation chamber (17) through the circular hole valve (21). At the same time, the microalgae cells are broken by the crushing blade (15), and then extracted for a certain period of time, so that the obtained organic phase is discharged from the sample discharge pipe port (18) below the oil preparation chamber (17) to obtain a crude oil extract, and the remaining algae residue is discharged from the sample discharge pipe port (18) together with the cleaned waste water.

7. The method for using the integrated device for microalgae cultivation, harvesting and oil preparation according to claim 6, characterized in that: The oil preparation chamber (17) is also used for the preliminary extraction of lutein, polysaccharides and protein active substances. The oil preparation method is to first add an oil extractant and then perform microalgae cell wall breaking treatment, followed by leaching, and the obtained organic phase is the crude oil extract.

8. The method for using the integrated device for microalgae cultivation, harvesting and oil preparation according to claim 6, characterized in that: The flocculant is prepared from one or more compounds selected from the group consisting of chitosan, sodium alginate, xanthan gum, starch and cellulose in appropriate proportions.

9. The method for using the integrated device for microalgae cultivation, harvesting and oil preparation according to claim 6, characterized in that: A temperature control device is provided below the oil preparation chamber (17), and the temperature control range is 0-80° C., which is used to control the optimal temperature required for microalgae cultivation and cell drying.

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