Laser-assisted transparent tape punching system and punching method

Abstract

The invention provides a laser-assisted transparent tape punching system and method, and belongs to the technical field of medical instruments. The system comprises a laser emission module, a guiding device and a microscope assembly, the laser emission module is arranged beside the microscope assembly and comprises a laser light source and an optical fiber collimator which are connected in sequence, the output end of the laser emission module faces the guiding device, and the laser emission module is configured to be capable of two-dimensionally moving relative to the light incident side of the guiding device. The guiding device comprises a concave reflector, and the concave reflector is arranged at an angle relative to the light emitting direction of the optical fiber collimator. The microscope assembly comprises a secondary reflector, an objective lens and a culture dish which are arranged in sequence, and the secondary reflector is arranged at the incident end of the microscope assembly and used for reflecting the incident light beam through the guide device to the objective lens and focusing the light beam on the culture dish. By the adoption of the system and the punching method, the adverse effect caused by the fact that an arc guide rail is adopted for conducting laser transparent belt punching operation in the related technology can be avoided, and the punching precision is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices, and in particular to a laser-assisted transparent band punching system and a punching method. Background Art

[0002] As the demand for assisted reproductive technology continues to grow, embryo culture technology has received more attention. The laser-assisted zona pellucida perforation system uses laser to ablate the zona pellucida, thereby effectively improving the embryo implantation rate and shortening the patient's successful pregnancy time.

[0003] Depending on the embryo sample, different areas of the zona pellucida often need to be ablated. Therefore, the device often needs a control module to achieve precise ablation of the infrared laser. In the related technology, the control module of the traditional laser zona pellucida perforator usually adopts an arc-shaped guide rail, which is used to drive the emitting end of the indicator laser and the ablation laser to move, and adjust the direction of the laser beam to achieve ablation of different positions of the zona pellucida.

[0004] The circular arc guide is used to move and guide the laser beam of the laser zona pellucida puncher. To adjust the position of the irradiation on the sample, a large space is required. The processing and layout of the corresponding circular arc guide and the corresponding equipment module are also limited and difficult. At the same time, relative to the adjustment of the laser focus position on the embryonic zona pellucida sample, the movement control scheme of the transmitter of the indicator laser and the ablation laser is also more complicated. If the setting specifications and space requirements of the circular arc guide cannot be met, the movement accuracy will be reduced, and the punching accuracy on the corresponding sample will also be reduced. Summary of the invention

[0005] The embodiment of the present invention provides a laser-assisted transparent tape punching system punching method, which can solve the adverse effects caused by the use of arc guide rails for laser transparent tape punching operations in related technologies and improve the punching accuracy. The technical solution is as follows: In a first aspect, an embodiment of the present invention provides a laser-assisted transparent band punching system, comprising: a laser emission module, a guide device and a microscope assembly, The laser emission module is arranged beside the microscope assembly, and comprises a laser light source and a fiber collimator connected in sequence, the output end of the laser emission module faces the guide device, and the laser emission module is configured to be able to move two-dimensionally relative to the light incident side of the guide device; The guiding device comprises a concave reflecting mirror, which is arranged at an angle relative to the light emitting direction of the optical fiber collimator and is used to guide the light beam emitted by the laser emitting module to irradiate the incident end of the microscope assembly; The microscope assembly comprises a secondary reflector, an objective lens and a culture dish which are arranged in sequence. The secondary reflector is arranged at the incident end of the microscope assembly and is used to reflect the light beam incident through the guide device to the objective lens and focus on the culture dish.

[0006] Optionally, the concave reflector is arranged at 45° relative to the light emitting direction of the optical fiber collimator, and the secondary reflector is arranged at 90° to the concave reflector. Optionally, the guiding device also includes a beam splitter, which is arranged below the output end of the laser emission module and between the concave reflector and the secondary reflector in the horizontal direction. The beam splitter is arranged at 45° relative to the light emitting direction of the fiber optic collimator, the concave reflector is parallel to the light emitting direction of the fiber optic collimator, and the secondary reflector is parallel to the beam splitter.

[0007] Optionally, the guiding device also includes a conical lens, which is arranged between the incident end of the microscope assembly and the output end of the laser emission module, the conical surface of the conical lens faces the microscope assembly, and the plane of the conical lens faces the laser emission module and is perpendicular to the light output direction of the optical fiber collimator. Optionally, the laser light source includes an indication laser light source and an infrared ablation laser light source, and the laser emission module further includes a fiber coupler, and the indication laser light source and the infrared ablation laser light source are connected to the fiber collimator via the fiber coupler.

[0008] Optionally, the wavelength range of the laser emitted by the indicator laser light source is 630-660 nm, and the wavelength range of the laser emitted by the infrared ablation laser light source is 1450-1500 nm.

[0009] Optionally, the secondary reflector is a dichroic mirror. In a second aspect, an embodiment of the present invention provides a punching method, which is implemented based on the laser-assisted transparent belt punching system described in the first aspect, and includes: Adjusting the relative position of the laser emission module and the guide device so that the light output side of the optical fiber collimator faces the light input side of the concave reflector; Starting the laser emission module, using the laser light source to emit laser light, which is collimated by the fiber collimator, reflected by the concave reflector, and irradiated to the incident end of the microscope assembly, and then reflected by the secondary reflector to the objective lens and finally focused on the culture dish to ablate the sample in the culture dish; The laser emission module is driven to move two-dimensionally relative to the light incident side of the guide device, and the position of the light beam irradiated on the concave reflector is changed to adjust the position of the light spot that is finally focused on the culture dish after passing through the secondary reflector and the objective lens, so as to ablate different positions of the sample.

[0010] The beneficial effects brought about by the technical solution provided by the embodiment of the present invention include at least: The laser-assisted transparent band punching system provided by the embodiment of the present invention optimizes the guiding mode and setting form of the laser emission module with a mobile guiding function, and is connected to the microscope component of the biological microscope structure as the main body through an electric slide rail or a slide structure to realize two-dimensional movement of the light incident side relative to the guiding device. It uses a guiding device mainly composed of a concave reflector to reflect and guide the light beam, and cooperates with the secondary reflector and objective lens in the microscope component to reflect and focus the light beam on the culture dish to perform directional ablation of the sample. It only needs to drive the laser emission module to perform a simple lateral two-dimensional movement to adjust the angle of the light beam with high precision to achieve punching at different positions on the sample. Compared with the traditional arc guide rail solution, it has higher movement and adjustment accuracy for the laser emission end such as the laser light source, and occupies and adjusts the required space volume, and has lower setting cost. It can solve the adverse effects caused by the use of arc guide rails for laser transparent band punching operations in related technologies and improve the punching accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0012] Figure 1 It is a structural schematic diagram of a laser-assisted transparent belt punching system provided by an embodiment of the present invention; Figure 2 It is a schematic diagram of laser drilling holes at different positions of a sample provided by an embodiment of the present invention; Figure 3 is a structural schematic diagram of a second laser-assisted transparent belt punching system provided by an embodiment of the present invention; Figure 4 is a structural schematic diagram of a third laser-assisted transparent belt punching system provided by an embodiment of the present invention; Figure 5 It is a flow chart of the punching method provided by an embodiment of the present invention.

[0013] In the figure: 1-laser emission module; 2-guiding device; 3-microscope assembly; 11-laser light source; 12-fiber collimator; 13-fiber coupler; 21-concave reflector; 22-beam splitter; 23-conical lens; 31-secondary reflector; 32-objective lens; 33-culture dish; 111-indicator laser light source; 112-infrared ablation laser light source. DETAILED DESCRIPTION In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0014] Figure 1 It is a structural schematic diagram of a laser-assisted transparent belt punching system provided by an embodiment of the present invention; Figure 2 It is a schematic diagram of laser drilling holes at different positions of a sample provided by an embodiment of the present invention; Figure 3 is a structural schematic diagram of a second laser-assisted transparent belt punching system provided by an embodiment of the present invention; Figure 4 Schematic diagram of the structure of the third laser-assisted transparent belt perforating system provided by the embodiment of the present invention. Figures 1 to 4 As shown, an embodiment of the present invention provides a laser-assisted transparent belt punching system, including: a laser emission module 1, a guide device 2 and a microscope assembly 3.

[0015] The laser emission module 1 is arranged beside the microscope assembly 3, and includes a laser light source 11 and a fiber collimator 12 connected in sequence. The output end of the laser emission module 1 faces the guide device 2, and the laser emission module 1 is configured to be able to move two-dimensionally relative to the light incident side of the guide device 2.

[0016] The guiding device 2 includes a concave reflector 21 . The concave reflector 21 is arranged at an angle relative to the light emitting direction of the optical fiber collimator 12 , and is used to guide the light beam emitted by the laser emission module 1 to irradiate the incident end of the microscope assembly 3 .

[0017] The microscope assembly 3 comprises a secondary reflector 31, an objective lens 32 and a culture dish 33 which are arranged in sequence. The secondary reflector 31 is disposed at the incident end of the microscope assembly 3 to reflect the incident light beam through the guide device 2 to the objective lens 32 and focus it on the culture dish 33.

[0018] In the embodiment of the present invention, the laser emission module 1 and the guide device 2 are structures independent of the microscope assembly 3, wherein the microscope assembly 3 can be a unit in a biological microscope integrated in a laboratory. When the embryo zona pellucida punching operation is not performed, it can be used as a part of the biological microscope itself, and the experimenter can observe the sample in the culture dish 33 through the eyepiece and other structures above the culture dish 33. The laser emission module 1 can be connected to the biological microscope as the main body through an electric slide rail or a slide table structure, and the guide device 2 can be fixedly mounted using a corresponding bracket structure to ensure that the laser emission module 1 can move horizontally in two dimensions relative to the light incident side of the guide device 2 under electric drive. Furthermore, the laser light source 11 inside the laser emission module 1 includes an indication laser light source 111 and an infrared ablation laser light source 112, and a fiber coupler 13 is also provided in the laser emission module 1, and the indication laser light source 111 and the infrared ablation laser light source 112 are connected to the fiber collimator 12 through the fiber coupler 13. The laser wavelength emitted by the indicator laser light source 111 is 650nm, and the laser wavelength emitted by the infrared ablation laser light source 112 is 1480nm. When the laser light source 11 is working, the indicator laser light source 111 first starts to emit invisible light with a wavelength of 650nm as the indicator laser to indicate the position where the ablation laser finally hits the sample in the culture dish 33. Then the indicator laser light source 111 is turned off, and the infrared ablation laser light source 112 is started to emit an ablation laser with a wavelength of 1480nm. After being coupled with the indicator laser by the optical fiber coupler 13 to unify the light output path, it is collimated by the optical fiber collimator 12 to form an output light beam, which is conducive to propagation in space.

[0019] In the first embodiment of the present invention, the laser emission module 1 is arranged beside the microscope assembly 3 in the horizontal direction, and the light emitting direction of the fiber optic collimator 12 thereof is downward in the vertical direction. The concave reflector 21 serving as the guide device 2 is arranged directly below the light emitting direction of the fiber optic collimator 12, and its arc concave side faces the laser emission module 1, and the whole is arranged at 45° relative to the light emitting direction of the fiber optic collimator 12. Correspondingly, the secondary reflector 31 located at the incident end of the microscope assembly 3 is arranged at 90° to the concave reflector 21. When the entire laser-assisted transparent band punching system is in the initial working condition, the light beam emitted by the laser emission module 1 hits the concave reflector 21 and travels along the concave reflector 21. Figure 1 After the straight optical path in the optical microscope assembly 3 is reflected to the incident end, it is reflected to the objective lens 32 through the secondary reflector 31 and focused on the culture dish 33, so as to realize the directional ablation of the first punching position of the sample in the culture dish 33. Furthermore, by driving the laser emission module 1 to move horizontally in two dimensions relative to the concave reflector 21, the light beam is hit at different positions of the concave reflector 21 and moves along the concave reflector 21. Figure 1The dotted light path trajectory is reflected at different angles to the incident end of the microscope assembly 3, and then reflected by the secondary reflector 31 to the objective lens 32 and focused on the culture dish 33, so as to reach the second punching position of the left dotted light path on the culture dish 33, or the third punching position of the right dotted light path on the culture dish 33, etc.

[0020] The laser-assisted transparent band punching system provided by the embodiment of the present invention optimizes the guiding mode and setting form of the laser emission module 1 with a mobile guiding function, and is connected to the microscope assembly 3 of the biological microscope structure as the main body through an electric slide rail or a slide structure to realize two-dimensional movement relative to the light incident side of the guide device 2. It uses a concave reflector 21 as the main guiding device 2 to reflect and guide the light beam, and cooperates with the secondary reflector 31 and the objective lens 32 in the microscope assembly 3 to reflect and focus the light beam on the culture dish 33 to perform directional ablation of the sample. It only needs to drive the laser emission module 1 to perform a simple lateral two-dimensional movement to adjust the angle of the light beam with high precision, so as to realize punching at different positions on the sample. Compared with the traditional arc guide rail solution, it has higher movement and adjustment accuracy for the laser emission end such as the laser light source 11, and occupies and adjusts the required space volume, and has lower setting cost. It can solve the adverse effects caused by the use of arc guide rails for laser transparent band punching operations in related technologies and improve the punching accuracy.

[0021] It should be noted that, in the embodiment of the present invention, the rear focal plane of the concave reflector 21 coincides with the rear focal plane of the objective lens 32, ensuring that the light spots at different positions on the concave reflector 21 are all converged onto the rear focal plane of the objective lens 32, and that the indicator laser and infrared laser will not deviate beyond the light aperture of the objective lens 32 when the two-dimensional slide and other structures drive the laser emission module 1 to move.

[0022] Further, for the schematic diagram of the laser entering the microscope assembly 3 drilling holes at different positions of the sample, refer to Figure 2 Different punching positions require light of different angles to enter the objective lens 32 at the rear focal plane of the objective lens 32, such as Figure 2 The solid line is the first punching position, and the dotted line is the second punching position. The parallel light beam at the first punching position passes through the objective lens 32 in the horizontal direction and is focused on the sample surface of the culture dish 33 to achieve punching. After the laser emission module 1 drives the laser to move in two dimensions, the switching between the first punching position and the second punching position is achieved through the reflection of the guide device 2. The parallel light at the second punching position forms a certain angle θ with the optical axis and is focused at a position with a height h at the center of the sample surface after passing through the objective lens 32. The corresponding relationship is: , Where f is the focal length of the objective lens, and θ is the angle between the parallel light beam and the optical axis.

[0023] Optionally, the guiding device 2 also includes a beam splitter 22, which is arranged below the output end of the laser emission module 1 and between the concave reflector 21 and the secondary reflector 31 in the horizontal direction. The beam splitter 22 is arranged at 45° relative to the light emitting direction of the fiber optic collimator 12, the concave reflector 21 is parallel to the light emitting direction of the fiber optic collimator 12, and the secondary reflector 31 is parallel to the beam splitter 22.

[0024] For example, in the second embodiment of the present invention, on the basis of the first embodiment, a beam splitter 22 and a concave reflector 21 are used to reflect the light beam emitted by the laser emission module 1. The position of the concave reflector 21 is shifted away from the incident end of the microscope assembly 3 in the horizontal direction, and its setting angle is changed so that it is parallel to the light emitting direction of the fiber collimator 12, that is, it is arranged vertically in the vertical direction. Accordingly, the beam splitter 22 is arranged directly below the output end of the laser emission module 1, and is arranged at 45° relative to the light emitting direction of the fiber collimator 12. The light beam emitted from the output end of the laser emission module 1 is reflected by the beam splitter 22, and then incident on the concave reflector 21 in a direction perpendicular to the concave reflector 21. After being reflected by the concave reflector 21, it hits the beam splitter 22 again. The beam splitter 22 can partially transmit and partially reflect the lasers with wavelengths of 650nm and 1480nm. The reflected laser passes through the beam splitter 22 and hits the incident end of the microscope assembly 3, and then is reflected by the secondary reflector 31 to the objective lens 32 and focused on the culture dish 33, so as to achieve directional ablation of the first punching position of the sample in the culture dish 33, refer to Figure 3 Correspondingly, by driving the laser emission module 1 to perform a simple lateral two-dimensional movement, the beam can be oriented with high precision to achieve drilling at different positions on the sample, as shown in FIG. Figure 3 The dotted light path in the figure. By adding a beam splitter 22 to cooperate with the concave reflector 21 to reflect and guide the laser, the concave reflector 21 does not need to be placed at a specific angle. The light beam emitted by the laser emission module 1 can be reflected by the beam splitter 22 and then hit the arc concave surface of the concave reflector 21 in a vertical direction for angle adjustment and reflection. Compared with the first embodiment, the light spot that is finally focused on the culture dish 33 after being reflected by the concave reflector 21 placed at a specific angle is usually elliptical in shape. In the second embodiment, the light spot that is finally focused on the culture dish 33 will be a more regular circle, and its light spot energy distribution will be more uniform, thereby improving the consistency and accuracy of the transparent band punching operation in the sample position.

[0025] Optionally, the guiding device 2 also includes a conical lens 23, which is arranged between the incident end of the microscope assembly 3 and the output end of the laser emission module 1, the conical surface of the conical lens 23 faces the microscope assembly 3, and the plane of the conical lens 23 faces the laser emission module 1 and is perpendicular to the light output direction of the optical fiber collimator 12. For example, reference Figure 4 In the third embodiment of the present invention, the arrangement form of the laser emission module 1 and the type of the matching guide device 2 are further optimized and changed. By placing the laser emission module 1 horizontally so that its output end is arranged horizontally, and setting the guide device 2 arranged between the laser emission module 1 and the microscope assembly 3 as a cone lens 23, the collimated light beam output by the laser emission module 1 will pass through and refract through the cone surface to the incident end of the microscope assembly 3 after hitting the plane of the cone lens 23, and finally focus on the culture dish 33 for directional ablation. Accordingly, the laser emission module 1 can be connected to the biological microscope as the main body through an electric slide rail or a slide table structure, and accordingly, its driving direction is changed to the longitudinal direction. By driving the laser emission module 1 to lift or descend, the light beam has been guided to achieve the change of the punching position. Compared with the first two embodiments, the laser emission module 1 is arranged in this form, and by placing it horizontally, the space occupied in the longitudinal direction after the laser emission module 1 is combined with the biological microscope as the main body can be further reduced, thereby improving the overall adaptability and practicality.

[0026] Optionally, in the third embodiment, the conical lens 23 may also be replaced by a convex lens, as long as accurate refraction of the collimated light beam output by the laser emitting module 1 can be achieved.

[0027] Optionally, the secondary reflector 31 is a dichroic mirror. Exemplarily, in the embodiment of the present invention, a dichroic mirror is used as the secondary reflector 31, and while ensuring that the light beams with wavelengths of 650nm and 1480nm can be reflected to the sample on the culture dish 33, the light beam illuminating the biological microscope above can be imaged through the dichroic mirror.

[0028] Furthermore, in another possible implementation of the embodiment of the present invention, the laser emission module 1 is driven to move two-dimensionally relative to the light incident side of the guide device 2. In addition to driving the laser emission module 1 to move as a whole through structures such as an electric slide rail or a slide structure, the optical path can also be adjusted by adjusting the position or angle of the concave reflector 21 or the conical lens 23 to achieve ablation at different positions on the final sample.

[0029] ‌ Figure 5 FIG. 1 is a flow chart of a punching method provided by an embodiment of the present invention. Figure 5 As shown, the embodiment of the present invention also provides a punching method based on Figures 1 to 4 The laser-assisted transparent band punching system shown is implemented, and the method includes the following steps: S1. Adjust the relative positions of the laser emission module 1 and the guide device 2 so that the light-emitting side of the optical fiber collimator 12 faces the light-incoming side of the concave reflector 21.

[0030] S2, start the laser emission module 1, use the laser light source 11 to emit laser, which is collimated by the fiber collimator 12, reflected by the concave reflector 21, and irradiated to the incident end of the microscope component 3, and then reflected by the secondary reflector 31 to the objective lens 32 and finally focused on the culture dish 33 to ablate the sample in the culture dish 33.

[0031] S3, drive the laser emission module 1 to move two-dimensionally relative to the light incident side of the guide device 2, change the position of the light beam irradiating the concave reflector 21, so as to adjust the position of the light spot that is finally focused on the culture dish 33 after passing through the secondary reflector 31 and the objective lens 32, so as to ablate different positions of the sample.

[0032] The laser-assisted zona pellucida punching system provided by the embodiment of the invention is used in conjunction with the above-mentioned punching method to perform directional ablation of the zona pellucida of the embryo sample. The guiding mode and setting form of the laser emission module 1 with a mobile guiding function are optimized, and the microscope assembly 3 of the biological microscope structure as the main body is connected through an electric slide rail or a slide structure to achieve two-dimensional movement relative to the light incident side of the guide device 2. The guide device 2, which is mainly a concave reflector 21, is used to reflect and guide the light beam, and the secondary reflector 31 and the objective lens 32 in the microscope assembly 3 are used to reflect and focus the light beam on the culture dish 33 to perform directional ablation of the sample. It is only necessary to drive the laser emission module 1 to perform a simple lateral two-dimensional movement to adjust the angle of the light beam with high precision, so as to achieve punching at different positions on the sample. Compared with the traditional arc guide scheme, it has higher movement adjustment accuracy for the laser emission end such as the laser light source 11, and occupies and adjusts the required space volume, and has lower setting cost. It can solve the adverse effects caused by the use of arc guides for laser zona pellucida punching operations in the related art, and improve the punching accuracy.

[0033] Unless otherwise defined, the technical terms or scientific terms used herein shall have the usual meanings understood by persons of ordinary skill in the field to which the present invention belongs. The words "first", "second" and similar words used in the patent application specification and claims of the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, words such as "one" or "one" do not indicate a quantity limitation, but indicate the existence of at least one. Words such as "include" or "comprise" and similar words mean that the elements or objects appearing before "include" or "comprise" cover the elements or objects listed after "include" or "comprise" and are extremely equivalent, and do not exclude other elements or objects. Words such as "connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0034] The above descriptions are merely optional embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A laser-assisted transparent band punching system, It is characterized in that include: Laser emission module (1), guide device (2) and microscope assembly (3), The laser emission module (1) is arranged beside the microscope assembly (3), and comprises a laser light source (11) and an optical fiber collimator (12) connected in sequence, the output end of the laser emission module (1) faces the guide device (2), and the laser emission module (1) is configured to be able to move in two dimensions relative to the light incident side of the guide device (2); The guiding device (2) comprises a concave reflecting mirror (21), the concave reflecting mirror (21) being arranged at an angle relative to the light emitting direction of the optical fiber collimator (12), and being used for guiding the light beam emitted by the laser emitting module (1) so as to irradiate the incident end of the microscope assembly (3); The microscope assembly (3) comprises a secondary reflector (31), an objective lens (32) and a culture dish (33) which are arranged in sequence; the secondary reflector (31) is disposed at the incident end of the microscope assembly (3) and is used to reflect a light beam incident through the guide device (2) to the objective lens (32) and focus the light beam on the culture dish (33).

2. The laser-assisted transparent band perforation system according to claim 1, It is characterized in that The concave reflector (21) is arranged at 45° relative to the light emitting direction of the optical fiber collimator (12), and the secondary reflector (31) is arranged at 90° to the concave reflector (21).

3. The laser-assisted transparent band perforation system according to claim 1, It is characterized in that The guiding device (2) further comprises a beam splitter (22), the beam splitter (22) being arranged below the output end of the laser emission module (1) and being located between the concave reflector (21) and the secondary reflector (31) in the horizontal direction, the beam splitter (22) being arranged at 45° relative to the light emitting direction of the optical fiber collimator (12), the concave reflector (21) being parallel to the light emitting direction of the optical fiber collimator (12), and the secondary reflector (31) being parallel to the beam splitter (22).

4. The laser-assisted transparent band perforation system according to claim 1, It is characterized in that The guide device (2) further comprises a conical lens (23), wherein the conical lens (23) is arranged between the incident end of the microscope assembly (3) and the output end of the laser emission module (1), the conical surface of the conical lens (23) faces the microscope assembly (3), and the plane of the conical lens (23) faces the laser emission module (1) and is perpendicular to the light output direction of the optical fiber collimator (12).

5. The laser-assisted transparent tape perforation system according to any one of claims 1 to 4, It is characterized in that The laser light source (11) comprises an indication laser light source (111) and an infrared ablation laser light source (112); the laser emission module (1) further comprises an optical fiber coupler (13); the indication laser light source (111) and the infrared ablation laser light source (112) are connected to the optical fiber collimator (12) via the optical fiber coupler (13).

6. The laser-assisted transparent band perforation system according to claim 5, It is characterized in that The wavelength range of the laser light emitted by the indicating laser light source (111) is 630-660 nm, and the wavelength range of the laser light emitted by the infrared ablation laser light source (112) is 1450-1500 nm.

7. The laser-assisted transparent band perforation system according to any one of claims 1 to 4, It is characterized in that The secondary reflector (31) is a dichroic mirror.

8. A punching method, implemented based on the laser-assisted transparent belt punching system according to any one of claims 1 to 7, It is characterized in that include: Adjusting the relative position of the laser emission module (1) and the guide device (2) so that the light-emitting side of the optical fiber collimator (12) faces the light-incoming side of the concave reflector (21); The laser emission module (1) is started, and the laser light source (11) is used to emit laser light, which is collimated by the optical fiber collimator (12), reflected by the concave reflector (21), and irradiated to the incident end of the microscope component (3), and then reflected by the secondary reflector (31) to the objective lens (32) and finally focused on the culture dish (33), thereby ablating the sample in the culture dish (33); The laser emission module (1) is driven to move two-dimensionally relative to the light incident side of the guide device (2), and the position of the light beam irradiated on the concave reflector (21) is changed to adjust the position of the light spot that is finally focused on the culture dish (33) after passing through the secondary reflector (31) and the objective lens (32), so as to ablate different positions of the sample.

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