Corrosion promoting and carbon sequestration microbial agent for livestock and poultry manure compost and application

Abstract

The invention discloses a preparation method of a microbial agent for promoting corrosion and sequestration of livestock and poultry manure and application of the microbial agent in aerobic composting of the livestock and poultry manure, the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure is prepared by adopting three strains of sphingobacterium thermophilum CICC 22013, lactobacillus plantarum-like CICC 24386 and paenibacillus polymyxa CICC 10580 and optimizing the culture and fermentation process of the three strains of bacteria, and the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure is prepared by adopting the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure and the application of the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure and the application of the microbial agent for promoting corrosion and sequestration of the livestock and poultry manure. The microbial inoculum has the remarkable characteristics of no antagonism between strains, stable effect, strong enzyme activity, high viable count and the like, the effective viable count is up to 5.0 * 10 < 9 >-1.0 * 10 < 10 > cfu / mL, functional metabolism of microorganisms in a compost microenvironment can be directionally regulated and controlled, biodegradation of organic matters is activated, compost humification is better promoted, carbon emission in the composting process is reduced, and the microbial inoculum has a good application prospect. And the method has important significance on improving the quality of compost products.

Description

Technical Field

[0001] The present invention belongs to the field of microbial technology, and specifically relates to a microbial agent capable of enhancing humification and carbon fixation of livestock and poultry manure compost and a preparation method thereof, as well as an application of increasing the total carbon and humus content in material products after livestock and poultry manure composting treatment. Background Art

[0002] Composting livestock and poultry manure is an economical and environmentally friendly resource utilization technology. Through the action of microorganisms, unstable organic carbon components in materials can be converted into stable humus. However, some carbon and nitrogen substances are inevitably lost in the traditional composting process, and the degree of humification is relatively low, which seriously affects the quality of compost products and aggravates greenhouse gas emissions. Therefore, how to promote the formation of humus (especially humic acid substances) more efficiently in a short time is an important goal of high-quality aerobic composting of livestock and poultry manure. The humification of organic matter such as sugars and proteins in the composting process is closely related to the decomposition of organic matter and the transformation of organic carbon, and is also considered to be a key step in composting carbon fixation. Microorganisms are the main driving force for material transformation in the composting process, with strong randomness and poor controllability. The addition of exogenous microbial agents can regulate the functional metabolism of microorganisms, activate the biodegradation of organic matter, and better promote the humification of compost, which is of great significance to improving the application value of compost products.

[0003] The invention patent with the existing application number 202310339562.8 discloses a composite microbial agent composed of Trichoderma longibrachiatum, Candida tropicalis and Geobacillus stearothermophilus in a mass ratio of (1-3): (1-3): (1-3). The agent can improve the degradation effect of livestock and poultry manure, and at the same time has an excellent deodorizing effect on livestock and poultry manure. However, the microbial agent mainly deals with the degradation of organic matter in livestock and poultry manure such as chicken manure and pig manure. The formation of precursor substances such as phenols, polysaccharides and amino acids after the degradation of organic matter in the material and the conversion of humus during the composting process are not mentioned. The invention patent with the existing application number 202110163698.9 uses the Bacillus subtilis ACCC60364, Bacillus pumilus ACCC04180, Bacillus tequila ACCC06525, and Mesophilic Sphingobacillus CICC22013 to degrade kitchen waste.

[0004] Xinjiang has a huge output of livestock and poultry manure, and the quantity and quality requirements of composting agents are increasing year by year. However, there is a lack of unified standards for the functions and applications of composting agents, composting processes, and humification determination. In addition, what kind of strains to use for compounding and what kind of processes to prepare microbial agents can solve the problems of low humification and difficult carbon emissions during livestock and poultry manure composting. The existing technical records do not provide an effective solution. For the selection of fermentation strains in the process of livestock and poultry manure composting, how to select a strain ratio that can ferment efficiently and play a synergistic role is the key to solving the problem. There is no prior art record about the compound fermentation system of three bacteria, namely, Sphingobacterium thalpophilum CICC 22013, Lentilactobacillus parafarraginis CICC 24386 and Paenibacillus polymyxa CICC 10580. There is also no prior art record about how to compound the three strains so that there is no antagonism between the strains, the effect is stable, the enzyme activity is strong and the number of live bacteria is large. Summary of the invention

[0005] In view of the problems of low humification degree and difficult to control carbon emissions in the process of livestock and poultry manure composting, and the lack of patents and literature reports on microbial agents specifically used for efficient humification and carbon fixation in livestock and poultry manure composting, the present invention provides a high-efficiency humification and carbon fixation agent for livestock and poultry manure composting and a preparation method. The present invention also provides the application of the livestock and poultry manure composting humification and carbon fixation agent in the directional humification of organic matter and the reduction of carbon emissions in livestock and poultry manure composting. By implementing the technical scheme of the present invention, the humification of livestock and poultry manure is enhanced, the carbon sequestration and directional conversion efficiency in the process of livestock and poultry manure composting is increased, the total organic carbon and soluble carbon content of the compost product are improved, and the problems of low humification degree and low product quality in livestock and poultry manure composting are solved.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions: The present invention provides a microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting, wherein the agent is composed of a mesophilic sphingobacillus ( 嗜土鞘氨醇杆菌 )CICC 22013, Lactobacillus lentus 副法拉第扁豆乳杆菌 )CICC 24386 and Paenibacillus polymyxa ( 类芽孢杆菌属 多粘类芽孢杆菌 )CICC 10580 fermentation broth.

[0007] In this application, the following content "Mesophilic Sphingobacterium ( 嗜土鞘氨醇杆菌 )CICC22013” ​​are abbreviated as “Mesophilic Sphingobacillus”; “Lactobacillus lentus (扁豆乳杆菌属 副法拉第(种) ) CICC 24386" are abbreviated as "Lactobacillus lentus"; "Bacillus polymyxa ( 多粘类芽孢杆菌 )CICC 10580" are abbreviated as "Bacillus polymyxa".

[0008] Furthermore, the present application provides a microbial agent for promoting the composting and fixing carbon of livestock and poultry manure, which is obtained by mixing the fermentation broth of mesophilic sphingobacillus, lactobacillus lentus and Bacillus polymyxa in a cell number ratio of 2:1:1.

[0009] Furthermore, the present invention provides a microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting, with an effective viable bacterial count of 5.0×10 9 ~1.0×10 10 cfu / mL.

[0010] The mesophilic sphingobacterium used in the present invention ( 嗜土鞘氨醇杆菌 )CICC 22013, Lactobacillus lentus 副法拉第扁豆乳杆菌 )CICC 24386 and Paenibacillus polymyxa ( 多粘类芽孢杆菌 ) The three strains of CICC 10580 are all harmless and safe strains allowed for use by the Ministry of Agriculture and Rural Affairs, and were purchased from the China Industrial Microbiology Culture Collection (CICC).

[0011] At the same time, the present invention provides a preparation method of a microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting, which specifically comprises the following steps: activating the strains in each freezing tube stored in a -80°C ultra-low temperature refrigerator, and respectively carrying out freezing tube-plate-shaking flask culture to obtain a primary seed liquid of each strain; continuing to statically expand the above seed liquid in a 50L fermentation barrel, wherein three strains of compound sphingobacillus, bran-like lactobacillus and polymyxa are fermented respectively to obtain fermentation liquid; after the fermentation of each of the above single strains is completed, the fermentation liquids of the three strains of compound sphingobacillus, bran-like lactobacillus and polymyxa are mixed according to the bacterial body number ratio to obtain.

[0012] Furthermore, in the preparation method of the carbon-fixing microbial agent for livestock and poultry manure composting provided by the present invention, the activation culture medium used for the mesophilic sphingobacillus and polymyxa bacillus is: 10.0 g / L peptone, 10.0 g / L beef extract, 5.0 g / L sodium chloride, 15.0 g / L agar, and the pH value is adjusted to 7.0-7.2 after dissolving in distilled water, and the mixture is sterilized and set aside; the activation culture medium used for the bran-like Lactobacillus lentus is: 10.0 g / L casein peptone, 10.0 g / L beef extract, 5.0 g / L yeast extract, 20.0 g / L glucose, 2.0 g / L potassium dihydrogen phosphate, 15.0 g / L agar, and the pH value is adjusted to 6.0-6.5 after dissolving in distilled water, and the mixture is sterilized and set aside.

[0013] Furthermore, in the preparation method of the microbial agent for promoting the decay and fixing carbon for livestock and poultry manure composting provided by the present invention, the culture medium for static fermentation of the three bacteria in a 50L fermentation barrel is: 3.0% molasses, 0.5% peptone, 0.1% K2HPO4, and distilled water, and the culture conditions of the three bacteria are room temperature, static, and closed culture.

[0014] The invention provides an application of a decomposition-promoting and carbon-fixing microbial agent for livestock and poultry manure composting in a process of efficient resource utilization of livestock and poultry manure.

[0015] The invention provides an application of a decay-promoting and carbon-fixing microbial agent for livestock and poultry manure composting in aerobic composting of livestock and poultry manure.

[0016] Furthermore, the present invention provides an application of a humus-promoting and carbon-fixing microbial agent for livestock and poultry manure composting in aerobic composting of livestock and poultry manure, wherein the application is to enhance humification and improve carbon fixation functions.

[0017] The invention provides an application of a microbial agent for promoting decay and fixing carbon for composting livestock and poultry manure in crop planting.

[0018] Furthermore, the present invention provides an application of a microbial agent for promoting the decay and fixing carbon for livestock and poultry manure composting in crop planting, wherein the application is for promoting the germination of tomato seeds.

[0019] Compared with the prior art, the present invention has the following characteristics: (1) The effective live bacteria count of the carbon-fixing bacteria agent for livestock and poultry manure composting provided by the present invention is as high as 5.0×10 9 ~1.0×10 10cfu / mL, all of which are harmless and safe strains allowed for use by the Ministry of Agriculture and Rural Affairs, can be purchased through other public channels such as the China Industrial Microbiology Culture Collection Center (CICC), and can be expanded and cultured using public strain culture media and culture methods. The three strains of the present invention are preferably cultured using a special culture medium, and after mixing according to the volume ratio, the strains have the following significant characteristics: no antagonism between the strains, stable effect, strong enzyme activity, high number of viable bacteria, etc.

[0020] (2) The carbon-fixing bacteria agent for livestock and poultry manure composting provided by the present invention can effectively reduce greenhouse gas emissions during the composting process and significantly increase the total organic carbon and humus content in the compost product. Compared with natural composting, the total CH4 gas emission and the total CO2 gas emission of livestock and poultry manure treated with the carbon-fixing bacteria agent in the present invention were reduced by 32.3% and 41.1% respectively; the total organic carbon content in the compost product increased by 11.8%, the soluble organic carbon content increased by 20.0%, and the humus content increased by 14.8%; indicating that the carbon-fixing bacteria agent for livestock and poultry manure composting provided by the present invention can effectively solve the problems of carbon emissions and low humification degree during livestock and poultry manure composting.

[0021] (3) The carbon-fixing bacteria agent provided by the invention has strong adaptability, can produce highly active polyphenol oxidase, peroxidase, methane oxidase and functional bacteria for regulating carbon metabolism in compost, and has the characteristics of new strain combination, no antagonism between strains, stable effect, strong enzyme activity, large number of live bacteria, high cellulose degradation rate, effective reduction of carbon emissions, and increased humus content. After 50 days of composting, the cow dung compost with the carbon-fixing bacteria agent of the invention added had a total nitrogen content of 45.8%, a total carbon content of 12.8%, a tomato seed germination rate of 14.7%, and a humus content of 16.5% compared with the control group compost without the bacteria agent. The results show that the inoculation of the bacteria agent of the invention effectively increases the nutrient content of the final product of cow dung compost, significantly reduces the CO2 and CH4 emissions during the composting process, reduces carbon loss, and promotes the humification degree of the compost. In addition, the carbon-fixing bacteria agent can effectively degrade mature livestock and poultry manure and remove plant toxicity during the application process, which is beneficial to the growth of crops.

[0022] (4) The present invention solves the problem of high carbon emissions due to organic matter degradation during livestock and poultry manure composting, and improves carbon conversion and humus formation in livestock and poultry manure composting. The preparation process of the humus-promoting carbon fixation bacterial agent provided by the present invention is simple, and after inoculation, carbon loss is effectively reduced, the humification process is promoted, and good ecological and economic benefits can be generated. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a graph showing the changes in temperature, total organic carbon content and dissolved organic carbon content during the cow dung composting process using livestock and poultry manure composting carbon-fixing bacteria.

[0024] Figure A shows the fermentation temperature change graph; Figure B shows the total organic carbon content change graph; Figure C shows the dissolved organic carbon content change graph.

[0025] Figure 2 This is a graph showing the changes in humus, humic acid and degree of humification during the cow dung composting process using livestock and poultry manure composting carbon-fixing bacteria.

[0026] Figure A shows the changes in humus; Figure B shows the changes in humic acid content; and Figure C shows the changes in the degree of humification.

[0027] Figure 3 The figure shows the changes of polyphenol oxidase, peroxidase and methane oxidase in the process of cow dung composting using livestock and poultry manure composting and carbon fixation bacteria.

[0028] Figure A shows the changes in polyphenol oxidase; Figure B shows the changes in peroxidase; and Figure C shows the changes in methane oxidase.

[0029] Figure 4 Shown is a graph showing the changes in total nitrogen content, total organic carbon content, and tomato seed germination rate of the bacterial agent of the present invention and commercially available products during cow dung composting.

[0030] Figure A shows the changes in total nitrogen content; Figure B shows the changes in total organic carbon content; and Figure C shows the changes in tomato seed germination rate. Figure 5 Shown is a graph showing the changes in CO2 emission rate, CH4 emission rate, and humus content between the bacterial agent of the present invention and commercially available products. Figure D shows the change of CO2 emission rate; Figure E shows the change of CH4 emission rate; Figure F shows the change of humus content. DETAILED DESCRIPTION

[0031] The following examples are used to further illustrate the content of the present invention, but should not be construed as limiting the present invention. Without departing from the spirit and essence of the present invention, modifications or substitutions made to the method, steps or conditions of the present invention all belong to the scope of the present invention.

[0032] The mesophilic sphingobacterium used in the present invention ( 嗜土鞘氨醇杆菌 )CICC 22013, Lactobacillus lentus 副法拉第扁豆乳杆菌 )CICC 24386 and Paenibacillus polymyxa ( 多粘类芽孢杆菌 ) The three strains of CICC 10580 are all harmless and safe strains allowed for use by the Ministry of Agriculture and Rural Affairs and can be purchased through other public channels such as the China Industrial Microbiology Collection Center (CICC).

[0033] In this application, the following examples contain "Mesophilic Sphingobacterium (鞘氨醇杆菌属 嗜土(种) )CICC 22013” ​​are abbreviated as “Mesophilic Sphingobacillus”; “Lactobacillus lentus ( 副法拉第扁豆乳杆菌 ) CICC 24386" are abbreviated as "Lactobacillus lentus"; "Bacillus polymyxa ( 多粘类芽孢杆菌 )CICC 10580" are abbreviated as "Bacillus polymyxa".

[0034] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are all commercially available unless otherwise specified.

[0035] Example 1: Preparation of a carbon-fixing bacterial agent for livestock and poultry manure composting The bacterial strains in each freezing tube stored in a -80°C ultra-low temperature refrigerator are activated, and are cultured in freezing tubes, flat dishes, and shake flasks to obtain the primary seed liquid of each bacterial strain; the above seed liquid is further statically expanded in a 10L fermentation barrel, and the three strains of mesophilic sphingobacillus, lentobacillus of cereal bran and Paenibacillus polymyxa are fermented separately to obtain fermentation liquid; after the fermentation of the above single strains is completed, the fermentation liquids of the three strains of mesophilic sphingobacillus, lentobacillus of cereal bran and Paenibacillus polymyxa are mixed to obtain. The activation culture medium used by the mesophilic sphingobacillus and the polymyxa bacillus is: 10.0 g / L peptone, 10.0 g / L beef extract, 5.0 g / L sodium chloride, 15.0 g / L agar, which are dissolved in distilled water and the pH value is adjusted to 7.0-7.2, and the mixture is sterilized for later use; the activation culture medium used by the bran-like lactobacillus is: 10.0 g / L casein peptone, 10.0 g / L beef extract, 5.0 g / L yeast extract, 20.0 g / L glucose, 2.0 g / L potassium dihydrogen phosphate, 15.0 g / L agar, which is dissolved in distilled water and the pH value is adjusted to 6.0-6.5, and the mixture is sterilized for later use.

[0036] The conditions for the seed culture of the mesophilic Sphingobacillus and Paenibacillus polymyxa are: 160 rpm / min, 36° C. for 36 h; the conditions for the fermentation of the Lactobacillus lentus seed liquid are: 180 rpm, 34° C. for 48 h.

[0037] Example 2: A carbon-fixing bacterial agent specifically used for composting livestock and poultry manure Based on Example 1, this example provides a carbon fixation bacterial agent for livestock and poultry manure composting, wherein the fermentation broth of the mesophilic sphingobacillus, the bran-like Lactobacillus lentus and the polymyxa bacillus is mixed in a cell number ratio of 2:1:1, and the effective viable cell count of the composting bacterial agent is 5.0×10 9 ~1.0×10 10cfu / mL.

[0038] The culture medium for static fermentation of the three strains of bacteria in a 50L fermentation barrel is: 3.0% molasses, 0.5% peptone, 0.1% K2HPO4, and distilled water. The culture conditions of the three strains of bacteria are room temperature, static, and closed culture.

[0039] Example 3: Antagonism between strains of composite fermentation agent This example is based on the example to the second example to verify whether there is antagonism between the mesophilic sphingobacillus, the lentus of Lactobacillus granarius and the polymyxa bacillus strains. The strains inoculated on the slopes of the respective test tubes are made into bacterial suspensions until the OD 600 =0.10~0.12. Add the prepared bacterial suspension as the background bacteria to the dissolved sterile solid culture medium at about 37°C, mix well and pour into the plate. After the plate is cooled and solidified, gently place the sterile Oxford cup on it with tweezers, and draw an equal amount of other strain bacterial suspensions into the Oxford cup according to different volumes. Each group of experiments is repeated 3 times, and sterile water is used to replace the bacterial suspension in the Oxford cup as a blank control. After culturing in the incubator for a period of time (bacterial culture 48h, fungi and actinomycetes culture 5d), observe whether an inhibition zone appears around the Oxford cup. If there is no inhibition zone, it means that there is no antagonism between the background bacteria and the inoculated strains. On the contrary, it means that there is antagonism between the strains. Among them, the volume ratio between the strains is the cell number ratio of mesophilic sphingobacillus (A), Lactobacillus lentus (B), and Bacillus polymyxa (C). For specific results, see Table 1.

[0040] Table 1: Antagonism test of different strains

[0041] Note: ++ indicates a strong antagonistic reaction; + indicates a weak antagonistic reaction; - indicates no antagonistic reaction.

[0042] From the data in Table 1, it can be seen that no inhibition zone appeared around the Oxford cup after the bacterial suspensions of different strains cultured in special culture media were mixed in pairs, indicating that there was no antagonism between the three strains of mesophilic Sphingobacillus, Lactobacillus lentus and Paenibacillus polymyxa, and the effect was significant.

[0043] Example 4: Application of a bacterial agent specifically for promoting the decomposition and fixing carbon in livestock and poultry manure composting (1) Changes in temperature, greenhouse gas emissions, and humus content during the cow dung composting process of the livestock and poultry manure composting carbon fixation agent of the present invention The compost material used in this embodiment is dehydrated cow dung (water content is 70-80%) and crushed cotton stalks from the Anningqu Experimental Field of Xinjiang Academy of Agricultural Sciences. According to 90kg of dehydrated cow dung, 30kg of crushed cotton stalks and 0.05% of microbial agent, the initial carbon-nitrogen ratio of the compost raw material is about 32%, and the water content is 65%. On the basis of Examples 1 to 3, the promotability and carbon fixation bacteria provided by the present invention were applied to cow dung composting, and the temperature, CH4 and CO2 gas emissions, TOC content changes and humus content changes of the promotability and carbon fixation bacteria (Group A) obtained by the formula and preparation method provided in Examples 1 and 2 of the present invention during cow dung composting were investigated. Commercially available composting agents (Group B, purchased from Zhengzhou Yuqi Fu Agricultural Technology Co., Ltd., organic material composting agents, using Bacillus amyloliquefaciens, Bacillus jelly-like, Aspergillus niger, etc.), mesophilic sphingobacillus (Group C), Lactobacillus plantarum (Group D), Bacillus polymyxa (Group E) and natural composting (CK Group) were used as controls. Each group was tested three times in parallel, and the other experimental conditions were the same. Relevant indexes were tested. For specific results, see Appendix Figure 1 and Table 2.

[0044] Table 2: Changes of relevant indicators during cow dung composting

[0045] By the attached Figure 1 It can be seen from the data recorded in that the maximum temperature of the first heating of the promoting and carbon-fixing bacteria agent obtained by the formula and preparation method provided in Example 1 and Example 2 of the present invention can reach 55°C, which is significantly higher than the commercially available decomposition agent and the control group. This shows that the promoting and carbon-fixing bacteria agent provided by the present invention heats up quickly after inoculation and maintains a high temperature for a long time. It can quickly kill the activity of insect eggs and pathogens in livestock and poultry manure and promote the decomposition process of materials.

[0046] The total organic carbon and humus content of cow dung compost were further analyzed. From the data in Table 2, it can be seen that during the application of the carbon fixation and decomposition promoting bacteria provided by the present invention, the total organic carbon content was 237.3 g / kg at the end of composting (55 days). Among them, it was 6.8% higher than the marketed decomposition agent of Group B, 11.8% higher than the natural decomposition of Group CK, 6.7% higher than the mesophilic sphingobacillus inoculum of Group C, 8.0% higher than the bran-like lactobacillus inoculum of Group D, and 8.3% higher than the polymyxa bacillus inoculum of Group E; the soluble carbon content was 1.3 g / kg, which was 34.4% higher than the marketed decomposition agent of Group B, 20.3% higher than the natural decomposition of Group CK, and 15.5% higher than the mesophilic sphingobacillus inoculum of Group C. , which was 22.1% higher than that of the bran-like Lactobacillus retardus inoculant in Group D and 21.7% higher than that of the polymyxa Bacillus inoculant inoculant in Group E; thus, it can be seen that the pro-corrosion and carbon-fixing bacterial agent provided by the present invention effectively promotes the conversion of organic carbon in the material during the composting process of livestock and poultry manure solid waste, significantly increases the content of total organic carbon and humus, solves the problem of carbon emission and low degree of humification of livestock and poultry manure organic solid waste due to carbon metabolism, and at the same time improves the carbon fixation and nutrient retention of livestock and poultry manure organic solid waste.

[0047] (2) The present invention provides an application of a carbon fixation bacteria agent for cow dung composting In this example, on the basis of Example 1 and Example 2, the promotability and carbon fixation bacteria agent provided by the present invention is applied to a tomato seed germination rate experiment after the cow dung compost is decomposed, and the effect of the promotability and carbon fixation bacteria agent (Group A) obtained by the formula and preparation method provided in Example 1 and Example 2 of the present invention on the germination rate of tomato seeds is investigated. Commercially available decomposition agents (Group B), mesophilic sphingobacillus (Group C), Lactobacillus lentus (Group D), Paenibacillus polymyxa (Group E) and natural decomposition (CK group) are used as controls. Each group is tested three times in parallel, and the other experimental conditions are the same. The specific data are shown in Table 3.

[0048] Table 3: Effects of the carbon-fixing bacteria on the germination rate of tomato seeds in cow dung compost

[0049] Based on the data recorded in Table 3, it can be seen from the germination rate of tomato seeds at different composting stages that the promotility and carbon fixation bacteria provided by the present invention, after being applied to livestock and poultry manure composting, is used for tomato planting, and its toxicity to plants is significantly reduced compared with natural decomposition (CK) and commercially available bacteria. After 4 days of composting, the germination rate of tomatoes increased by 5% compared with the naturally decomposed CK group, 1% compared with the commercial bacteria in Group B, 3% compared with the mesophilic sphingobacillus inoculum inoculum in Group C, 6% compared with the bran-like Lactobacillus inoculum inoculum in Group D, and 2% compared with the polymyxa-paenibacillus inoculum inoculum in Group E; after 11 days, the germination rate of tomatoes increased by 12% compared with the naturally decomposed CK group, 8% compared with the commercial bacteria in Group B, 6% compared with the mesophilic sphingobacillus inoculum in Group C, 7% compared with the bran-like Lactobacillus inoculum inoculum in Group D, and 4% compared with the polymyxa-paenibacillus inoculum inoculum in Group E. %; At 20 days, the germination rate of tomatoes increased by 15% compared with the naturally mature CK group, 8% compared with the commercially available group B, 9% compared with the mesophilic sphingobacillus inoculant in C, 11% compared with the bran-like Lactobacillus inoculant in D, and 6% compared with the polymyxa bacillus inoculant in E; At 55 days, the germination rate of tomatoes increased by 16% compared with the naturally mature CK group, 7% compared with the commercially available group B, 10% compared with the mesophilic sphingobacillus inoculant in C, 12% compared with the bran-like Lactobacillus inoculant in D, and 6% compared with the polymyxa bacillus inoculant in E. 多粘类芽孢杆菌 ) increased by 6%. This shows that the decomposition-promoting carbon-fixing bacterial agent provided by the present invention can effectively degrade mature livestock and poultry manure and remove plant toxicity during application, which is beneficial to the growth of crops.

[0050] The above examples show that Figure 3 According to the records in the literature, the carbon fixation bacteria agent provided by the present invention has strong adaptability and can produce highly active cellulase, lignin degrading enzyme, polyphenol oxidase, α-glucosidase, methane oxidase and functional bacteria for regulating carbon metabolism in composting, with the characteristics of new strain combination, no antagonism between strains, stable effect, strong enzyme activity, large number of live bacteria, high degradation rate of lignocellulose, effective reduction of carbon emissions, and increase of humus content. At the end of composting, the humic acid content of livestock and poultry manure and cattle dung increased by 14.8% compared with the naturally decomposed CK group, 11.6% compared with the marketed composting agent of group B in the city, 8% compared with the mesophilic sphingobacillus inoculant of group C, 16.4% compared with the grain bran-like retardant lactobacillus inoculant of group D, and 7.3% compared with the polymyxa bacillus inoculant of group E; in addition, the humification degree (HA / FA) ​​increased by 6.8% compared with the naturally decomposed CK group, 15.0% compared with the marketed composting agent of group B in the city, 13.1% compared with the mesophilic sphingobacillus inoculant of group C, 14.6% compared with the grain bran-like retardant lactobacillus inoculant of group D, and 11.3% compared with the polymyxa bacillus inoculant of group E. The present invention solves the problem of high carbon emission due to organic matter degradation during livestock and poultry manure composting, and improves carbon conversion and humus formation in livestock and poultry manure composting. The preparation process of the humus-promoting carbon fixation bacterial agent provided by the present invention is simple, and after inoculation, carbon loss is effectively reduced, the humification process is promoted, and good ecological and economic benefits can be generated.

[0051] Example 5: Application of a bacterial agent specifically for promoting the decomposition and fixing carbon in livestock and poultry manure composting Composting comparison test of the microbial agent of the present invention. The test site is the Anningqu Experimental Station of the Xinjiang Academy of Agricultural Sciences. The microbial agent for promoting decay and fixing carbon obtained by the formula and preparation method provided in Example 1 and Example 2 of the present invention is mainly used. The microbial agent of the present invention is added at a volume weight ratio of 1‰, mixed evenly with cow dung, and the moisture content of the compost raw material is adjusted to about 65%. The initial carbon-nitrogen ratio of the compost material is adjusted to 30 using crushed cotton straw, and a pile with a width of 0.8 meters, a height of 0.6 meters, and a length of 1.0 meters is built for composting and fermentation. At the same time, a natural compost without the addition of microbial agent is set as a control group (CK group).

[0052] The main composting effects are shown in the attached Figure 4 The record in the literature states that at the end of composting (50 days), the total nitrogen content of the cow dung compost added with the inventive humus-fixing bacterial agent increased by 45.8%, the total carbon content increased by 12.8%, the tomato seed germination rate increased by 14.7%, and the humus content increased by 16.5% compared with the control group compost without the inventive bacterial agent. The results show that the inoculation of the inventive bacterial agent effectively increases the nutrient content of the final product of cow dung compost, significantly reduces the emission rate of CO2 and CH4 during the composting process, reduces carbon loss, and promotes the humification degree of the compost.

[0053] The above embodiments are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the present invention.

Claims

1. A microbial agent for promoting the decomposition and carbon fixation of livestock and poultry manure composting, characterized in that: The bacterial agent is composed of mesophilic sphingobacterium ( Sphingobacterium thalpophilum )CICC 22013, Lactobacillus lentus ( Lentilactobacillus parafarraginis ) CICC 24386 and Paenibacillus polymyxa ( Paenibacillus polymyxa ) CICC 10580 fermentation broth was mixed.

2. The microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting according to claim 1, characterized in that: The bacterial agent is composed of mesophilic sphingobacterium ( Sphingobacterium thalpophilum )CICC 22013, Lactobacillus lentus ( Lentilactobacillus parafarraginis )CICC 24386 and Paenibacillus polymyxa ( Paenibacillus polymyxa ) CICC 10580 fermentation broth was obtained by mixing the bacterial cell number ratio of 2:1:

1.

3. The microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting according to claim 1, characterized in that: The effective viable bacteria count of the bacterial agent is 5.0×10 9 ~1.0×10 10 cfu / mL.

4. A method for preparing a microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting according to claim 1, characterized in that: Specifically, the steps include: activating the strains in each cryovial stored in a -80°C ultra-low temperature freezer, and performing cryovial-plate-shake flask culture to obtain the primary seed liquid of each strain; statically culturing the above seed liquid in a 50L fermentation tank at a 2% inoculation rate to obtain the fermentation stock liquid, and isolating the thermophilic Sphingobacterium ( Sphingobacterium thalpophilum )CICC 22013, Lactobacillus lentus ( Lentilactobacillus parafarraginis )CICC 24386 and Paenibacillus polymyxa ( Paenibacillus polymyxa ) CICC 10580 The three fermentation broths were mixed in the above proportions.

5. The method for preparing the microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting according to claim 4, characterized in that: The mesophilic sphingobacterium ( Sphingobacterium thalpophilum ) CICC 22013 and Paenibacillus polymyxa ( Paenibacillus polymyxa ) The activation medium used for CICC 10580 is: peptone 10.0 g / L, beef extract 10.0 g / L, sodium chloride 5.0 g / L, agar 15.0 g / L, dissolved in distilled water and adjusted to pH 7.0-7.2, sterilized and ready for use; Lactobacillus lentus ( Lentilactobacillus parafarraginis The activation medium used for CICC 24386 is: casein peptone 10.0 g / L, beef extract 10.0 g / L, yeast extract 5.0 g / L, glucose 20.0 g / L, potassium dihydrogen phosphate 2.0 g / L. Adjust the pH to 6.0-6.5 and sterilize before use.

6. The method for preparing the microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting according to claim 4, characterized in that: The mesophilic sphingobacterium ( Sphingobacterium thalpophilum ) CICC 22013 and Paenibacillus polymyxa ( Paenibacillus polymyxa ) CICC 10580 seed culture conditions: 160 rpm / min, 36 ℃ for 36 h; Lactobacillus lentus ( Lentilactobacillus parafarraginis ) CICC 24386 seed liquid fermentation culture conditions are: 180 rpm, 34 ° C for 48 h.

7. The method for preparing the microbial agent for promoting the decomposition and fixing carbon for livestock and poultry manure composting according to claim 4, characterized in that: The culture medium for static fermentation of the three bacteria in a 50L fermentation barrel is: 3.0% molasses, 0.5% peptone, 0.1% K2HPO4, and distilled water. The culture conditions of the three bacteria are room temperature, static, and closed culture.

8. Use of the microbial agent for promoting the decay and fixing carbon for livestock and poultry manure composting according to any one of claims 1 to 3 in aerobic composting of livestock and poultry manure.

9. Use of the microbial agent for promoting the decay and fixing carbon in livestock and poultry manure composting according to claim 8 in aerobic composting of livestock and poultry manure, characterized in that: The application is to enhance humification and improve carbon fixation functions.

10. Use of the microbial agent for promoting the decomposition and carbon fixation of livestock and poultry manure composting according to any one of claims 1 to 3 in crop planting.

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