A parasitic egg magnetization measuring device and a parasitic egg magnetization measuring method during artificial breeding of trichogrammatids

Abstract

The invention discloses a parasitic egg magnetization measuring device and a method for measuring the magnetization of parasitic eggs in the artificial breeding process of trichogrammatids, belonging to the field of trichogrammatid breeding devices. The purpose of the invention is to solve the existing problem of over-parasitism of trichogrammatids. After the parasitic eggs that have been parasitized are measured by the magnetization measuring method, the parasitic eggs are separated from the unparasitized host eggs, the unparasitized host eggs are repeatedly parasitized and ovulated, and the parasitic eggs are cultured, so as to realize the control of the number of times the host eggs are parasitized, reduce the over-parasitism ratio, and solve the occurrence of the over-parasitism problem. And it can significantly improve the body length and uniformity, eclosion rate, life span, etc. of trichogrammatids. In addition, the invention performs parasitism measurement through a device and facilitates manual intervention in the number of parasitism. The measurement method is highly reliable and efficient, and there is no time lag.

Description

Technical Field

[0001] The invention belongs to the field of trichogrammatid breeding devices, and in particular relates to a magnetization measuring device and a magnetization measuring method for parasitic eggs in the process of artificial breeding of trichogrammatids. Background Art

[0002] Trichogrammatids are parasitic natural enemies of many major lepidoptera pests in crops such as rice, corn, cotton, sugarcane, vegetables, and jasmine, and have a significant control effect on target pests. The types of Trichogrammatids that can be bred and released on a large scale in my country mainly include Trichogrammatids of rice borers, Trichogrammatids of yellow stem borers, Trichogrammatids of pine caterpillars, and Trichogrammatids of corn borers. In crop production, artificially bred Trichogrammatids are released in large areas in the field, which can significantly reduce the population of pests by parasitizing the eggs of target pests, and effectively prevent and control pests such as the striped stem borer, rice leaf roller, corn borer, cotton bollworm, sugarcane borer (striped stem borer, two-spotted stem borer, yellow stem borer), and jasmine bud borer. my country has a long history of development and successful experience in the use of artificial breeding and release of Trichogrammatids to control pests, as well as good technical and industrial support. Artificial breeding and release of Trichogrammatids began in the 1970s, forming an integrated system of scientific research, production, and application. The technology of artificially releasing trichogrammatids to control pests can significantly reduce the population size and degree of damage of target pests, and has a good pest control effect. During the egg stage of target pests, trichogrammatids are released 1-2 times per generation, and the general egg parasitism rate can reach 60-70%, the control effect is 80-90%, and the application of pesticides can be reduced by 1-2 times. Due to the reduction in the use of pesticides, the population of natural enemies can be protected, the continuous pest control effect of natural enemies can be brought into play, the ecological environment of farmland can be improved, the degree of pest occurrence can be continuously reduced, the number of control times and the amount of pesticides used can be reduced, the quality of agricultural products can be improved, and the planting benefits can be increased.

[0003] The dominant bee species in nature are artificially bred in large numbers through hosts, and qualified parasitic eggs of trichogrammatids are obtained and then released in large numbers. At present, it has been confirmed that trichogrammatids with good control effects and can be bred in factories include using trichogrammatids of rice borer to control rice stem borer and rice leaf roller, artificially bred using rice moth eggs; using trichogrammatids of pine caterpillars to control corn borer, artificially bred using tussah eggs, or using trichogrammatids of corn borer, artificially bred using rice moth or wheat moth eggs; using trichogrammatids of yellow stem borer to control cotton bollworm, jasmine bud borer and sugarcane borer, artificially bred using tussah eggs.

[0004] However, the problem of overparasitism of trichogrammatids is still widespread and has not been effectively solved. Overparasitism of trichogrammatids refers to the number of individuals of the parasitoid trichogrammatids in the host eggs exceeding the number that the host can bear, resulting in the death of some or all individuals due to incomplete development, or a series of problems due to poor development, such as the inability of all or some individuals to complete development. Due to nutritional problems, the offspring that have completed development will have a decrease in the emergence rate, size, lifespan, fertility and female ratio, and in severe cases, the death of the parasitic bee. The size of a tussah egg is about 0.5~2 mm, sesame-shaped, and one egg can support the development of about 80 trichogrammatids. Therefore, it is difficult for people to determine whether overparasitism has occurred when using tussah eggs as hosts. The direct manifestation of overparasitism is that the parasitoid trichogrammatids parasitize and ovulate twice or more in the same tussah egg host, so that young bees with different developmental periods will be formed in the host egg, which increases the inconsistency of the final individual development. All these will affect the quantity and quality of Trichogrammatid bees during artificial breeding. There is currently no good artificial intervention method for controlling over-parasitism of Trichogrammatid bees. It only relies on the parasitoid Trichogrammatid to autonomously identify whether the host has been parasitized or to determine whether it has been parasitized by the color change of the host eggs. However, these methods are less reliable. It takes about ten hours to determine whether it has been parasitized by the color change of the host eggs. There is a certain time lag and low efficiency. Summary of the invention

[0005] In order to solve the existing over-parasitism problem of trichogrammatids, the present invention proposes a magnetization measurement method for parasitic eggs in the artificial breeding process of trichogrammatids. After the parasitic eggs that have been parasitized are measured by the magnetization measurement method, the parasitic eggs are separated from the unparasitized host eggs, the unparasitized host eggs are repeatedly parasitized and ovulated, and the parasitized eggs are cultured, thereby realizing the control of the number of times the host eggs are parasitized, reducing the over-parasitism ratio, and solving the occurrence of the over-parasitism problem.

[0006] The parasitic egg magnetization measuring device of the present invention is composed of a reactor, a hot air blower, an argon gas bottle, a pure water storage tank, an artificial yolk liquid storage tank, a support plate driving control box and a wastewater storage tank; a discharge port is opened on the upper part of the side wall of the reactor, and a valve is arranged on the discharge port; a spray head is arranged on the top of the reactor, and the spray head is connected to the water outlet pipe of the pure water storage tank arranged outside the reactor; a hot air pipe is arranged on the top of the reactor, and the hot air pipe is connected to the exhaust pipe of the hot air blower arranged outside the reactor; a high-pressure gas inlet is arranged on the side wall of the reactor, and the high-pressure gas inlet is connected to the exhaust pipe of the hot air blower arranged outside the reactor; The reactor is connected to the outlet pipe of the argon gas bottle outside the reactor; the bottom of the reactor is provided with an artificial yolk liquid inlet, an artificial yolk liquid outlet and a wastewater outlet, the artificial yolk liquid inlet is connected to the artificial yolk liquid delivery pipe of the artificial yolk liquid storage tank outside the reactor, the artificial yolk liquid outlet is connected to the artificial yolk liquid recovery pipe of the artificial yolk liquid storage tank, and the wastewater outlet is connected to the water inlet pipe of the wastewater storage tank outside the reactor; a supporting plate is provided in the reactor, the supporting plate is a circular plate, the supporting plate is inclined, the supporting plate is a porous plate, and the edge of the supporting plate is tightly connected to the inner wall of the reactor. The pallet is in contact with the reactor, and a vertical pallet rotating shaft is arranged at the center of the lower surface of the pallet; the pallet driving control box is arranged at the bottom of the reactor and the pallet driving control box is sealed and connected to the bottom of the reactor; a pallet rotating shaft driving motor and a pallet lifting driving motor are arranged in the pallet driving control box, the lower end of the pallet rotating shaft passes through the rotating shaft through hole at the center of the bottom plate of the reactor and is connected to the power output shaft of the pallet rotating shaft driving motor, and two guide plates are symmetrically arranged on both sides of the pallet rotating shaft driving motor, and the two ends of the guide plates are respectively connected to the bottom of the reactor and the bottom of the pallet driving control box. The guide plate is provided with a vertical T-shaped slot; two horizontal connecting plates are symmetrically arranged on the housing of the pallet rotating shaft driving motor, one end of the connecting plate is fixedly connected to the housing of the pallet rotating shaft driving motor, and the other end of the connecting plate is fixedly connected with a slider, which is arranged in the T-shaped slot; a vertical threaded through hole is arranged on the connecting plate, the pallet lifting driving motor is fixed on the bottom plate inside the pallet driving control box, a vertical screw is connected to the power output shaft of the pallet lifting driving motor, and the screw is arranged in the vertical thread of the connecting plate; the electromagnet is arranged on the bottom plate inside the reactor.

[0007] The present invention uses a parasitic egg magnetization measuring device to carry out a magnetization measuring method for parasitic eggs in the artificial breeding process of trichogrammatids according to the following steps:

[0008] Step 1, placing trichogrammatid bee species and host eggs in a culture room for bee inoculation; the number ratio of trichogrammatid bee species to host eggs is 1:50-60; the bee inoculation time is 5 hours, wherein, before the 4th hour, the temperature in the culture room is controlled at 10-15° C., and the humidity is 65-75%; from the 4th hour to the 5th hour, the temperature in the culture room is controlled at 23-25° C., and the humidity is 65-75%;

[0009] Step 2, separating the trichogrammatid bee species and the host eggs, collecting the host eggs, and performing a magnetization measurement of the host eggs using a parasitic egg magnetization measurement device, and determining the host eggs that have been parasitized in the collected host eggs, namely the parasitic eggs;

[0010] The magnetization determination method of the parasitic egg using the parasitic egg magnetization determination device is as follows:

[0011] ①, placing the collected host eggs in a reactor in a parasitic egg magnetization measuring device and stacking them on a support plate; transporting the artificial yolk liquid in the artificial yolk liquid storage tank into the reactor and immersing the host eggs, and at the same time using an argon gas bottle to transport high-pressure argon gas into the reactor. After the air pressure in the reactor reaches 2-3 MPa, start the support plate rotary shaft drive motor to rotate the support plate to stir the host eggs, stop after 1-2 hours, and stop transporting the high-pressure argon gas, and finally release the air pressure in the reactor to normal pressure;

[0012] The artificial yolk solution is prepared by mixing glucose, tussah egg yolk, carboxylated ferric oxide nanoparticles and purified water;

[0013] ②, empty the artificial yolk liquid in the reactor, and use the purified water in the pure water storage tank to rinse the host eggs through the spray head, and then empty the purified water in the reactor after rinsing;

[0014] ③. Use a hot air blower to dry the host eggs in the reactor at a drying temperature of 20-25°C;

[0015] ④, injecting the purified water in the purified water storage tank into the reactor through the spray head until the host eggs are submerged and the liquid level is higher than the discharge port, and then adding sucrose to the purified water in the reactor to obtain a sucrose solution;

[0016] The mass fraction of sucrose in the sucrose solution is 10%;

[0017] ⑤. Start the support plate rotary shaft drive motor to rotate the support plate to stir the host eggs, and at the same time turn on the power of the electromagnet. Under the magnetic force, the host eggs floating in the sucrose solution are stratified, some host eggs move toward the support plate to the lower layer of the sucrose solution, and some host eggs float on the upper layer of the sucrose solution. The host eggs in the lower layer are parasitic eggs.

[0018] The principles and beneficial effects of the present invention are:

[0019] 1. The magnetization measurement method for parasitic eggs in the artificial breeding process of trichogrammatids of the present invention measures the parasitic eggs that have been parasitized by the magnetization measurement method and separates the parasitic eggs from the unparasitized host eggs, which can effectively control the number of parasitism, avoid the occurrence of repeated parasitism, achieve effective control of over-parasitism, significantly reduce the over-parasitism ratio, and thus ensure that the consistency of individual development in the parasitic eggs is improved; the present invention performs parasitism measurement through a device and facilitates manual intervention in the number of parasitism, the measurement method has high reliability and efficiency, and there is no time lag.

[0020] 2. During the process of Trichogrammatid bees being attached to host eggs, Trichogrammatid bees use the ovipositor at the end of the abdomen to drill into the host egg to form a channel and lay eggs in the host egg. In the present invention, the artificial yolk liquid enters the host egg through the channel formed during the bee attachment process under the high pressure in the reactor; the artificial yolk liquid contains glucose, tussah egg yolk, and carboxylated ferric oxide nanoparticles. The tussah egg yolk is used to supplement the yolk of the host egg, and the carboxylated ferric oxide nanoparticles are magnetic particles with good biocompatibility. The carboxylated ferric oxide nanoparticles are used to make the host egg magnetic, and then the host egg that is parasitized is measured and separated using a magnetic field. The artificial yolk liquid containing glucose and tussah egg yolk is beneficial to the individual development of Trichogrammatid bees, and can significantly improve the body length and uniformity, emergence rate, life span, etc. of Trichogrammatid bees. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a schematic diagram of the structure of the parasitic egg magnetization measuring device in Example 1;

[0022] Figure 2 This is a schematic diagram of the connection between the connecting plate and the guide plate in Example 1;

[0023] Figure 3 This is a cross-sectional view of the guide plate in Example 1. DETAILED DESCRIPTION

[0024] The technical solution of the present invention is not limited to the specific implementation modes listed below, but also includes any reasonable combination of the specific implementation modes.

[0025] Specific implementation method one: The parasitic egg magnetization measuring device of this implementation method is composed of a reactor 1, a hot air blower 2, an argon gas bottle 3, a pure water storage tank 4, an artificial yolk liquid storage tank 5, a support plate drive control box 6 and a wastewater storage tank 7; a discharge port 8 is opened on the upper part of the side wall of the reactor 1, and a valve is arranged on the discharge port 8; a spray head 9 is arranged on the top of the reactor 1, and the spray head 9 is connected to the water outlet pipe of the pure water storage tank 4 arranged outside the reactor 1; a hot air duct is arranged on the top of the reactor 1, and the hot air duct is connected to the exhaust duct of the hot air blower 2 arranged outside the reactor 1; a high-pressure gas inlet is arranged on the side wall of the reactor 1, and the high-pressure gas inlet is connected to the exhaust pipe of the hot air blower 2 arranged outside the reactor 1 The bottom of the reactor 1 is provided with an artificial yolk liquid inlet, an artificial yolk liquid outlet and a wastewater outlet, the artificial yolk liquid inlet is connected to the artificial yolk liquid delivery pipe of the artificial yolk liquid storage tank 5 arranged outside the reactor 1, the artificial yolk liquid outlet is connected to the artificial yolk liquid recovery pipe of the artificial yolk liquid storage tank 5, and the wastewater outlet is connected to the water inlet pipe of the wastewater storage tank 7 arranged outside the reactor 1; a support plate 10 is provided in the reactor 1, the support plate 10 is a circular plate, the support plate 10 is inclined, the support plate 10 is a porous plate, the edge of the support plate 10 is in close contact with the inner wall of the reactor 1, and the lower surface of the support plate 10 A vertical pallet rotary shaft 11 is arranged in the center; a pallet drive control box 6 is arranged at the bottom of the reactor 1 and the pallet drive control box 6 is sealed and connected to the bottom of the reactor 1; a pallet rotary shaft drive motor 13 and a pallet lifting drive motor 17 are arranged in the pallet drive control box 6, the lower end of the pallet rotary shaft 11 passes through the rotary shaft through hole in the center of the bottom plate of the reactor 1 and is connected to the power output shaft of the pallet rotary shaft drive motor 13, two guide plates 16 are symmetrically arranged on both sides of the pallet rotary shaft drive motor 13, and the two ends of the guide plates 16 are respectively connected to the bottom of the reactor 1 and the bottom of the pallet drive control box 6, and a guide plate 16 is arranged There is a vertical T-shaped slot 18; two horizontal connecting plates 14 are symmetrically arranged on the shell of the pallet rotating shaft driving motor 13, one end of the connecting plate 14 is fixedly connected to the shell of the pallet rotating shaft driving motor 13, and the other end of the shell of the connecting plate 14 is fixedly connected with a slider, and the slider is arranged in the T-shaped slot 18; a vertical threaded through hole is arranged on the connecting plate 14, and the pallet lifting driving motor 17 is fixed on the bottom plate inside the pallet driving control box 6, and a vertical screw 15 is connected to the power output shaft of the pallet lifting driving motor 17, and the screw 15 is arranged in the vertical thread of the connecting plate 14; the electromagnet 12 is arranged on the bottom plate inside the reactor 1.

[0026] This embodiment has the following beneficial effects:

[0027] The parasitic egg magnetization measuring device of the present embodiment measures the parasitic eggs that have been parasitized by the magnetization measuring method and separates the parasitic eggs from the unparasitized host eggs, which can effectively control the number of parasitism, avoid the occurrence of repeated parasitism, significantly reduce the proportion of over-parasitism, and achieve effective control of over-parasitism, thereby ensuring that the consistency of individual development in the parasitic eggs is improved; the present embodiment performs parasitism measurement through the device and facilitates manual intervention in the number of parasitism. The measurement method has high reliability and efficiency, and there is no time lag.

[0028] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that valves are provided on the water outlet pipe of the pure water storage tank 4, the exhaust pipe of the hot air blower 2, the air outlet pipe of the argon gas bottle 3, the artificial egg yolk liquid delivery pipe of the artificial egg yolk liquid storage tank 5, the artificial egg yolk liquid recovery pipe of the artificial egg yolk liquid storage tank 5 and the water inlet pipe of the wastewater storage tank 7.

[0029] Specific implementation method three: This implementation method is different from specific implementation methods one or two in that: the surface of the electromagnet 12 is sealed and coated with epoxy resin.

[0030] Specific embodiment 4: This embodiment is different from any one of specific embodiments 1 to 3 in that a mechanical seal is provided between the support plate rotary shaft 11 and the rotary shaft through hole at the center of the bottom plate of the reactor 1 .

[0031] Specific implementation method 5: In this implementation method, the magnetization measurement method of parasitic eggs in the artificial breeding process of trichogrammatids using a parasitic egg magnetization measurement device is carried out according to the following steps:

[0032] Step 1, placing trichogrammatid bee species and host eggs in a culture room for bee inoculation; the number ratio of trichogrammatid bee species to host eggs is 1:50-60; the bee inoculation time is 5 hours, wherein, before the 4th hour, the temperature in the culture room is controlled at 10-15° C., and the humidity is 65-75%; from the 4th hour to the 5th hour, the temperature in the culture room is controlled at 23-25° C., and the humidity is 65-75%;

[0033] Step 2, separating the trichogrammatid bee species and the host eggs, collecting the host eggs, and performing a magnetization measurement of the host eggs using a parasitic egg magnetization measurement device, and determining the host eggs that have been parasitized in the collected host eggs, namely the parasitic eggs;

[0034] The method for measuring the magnetization of host eggs by using the parasitic egg magnetization measuring device is as follows:

[0035] ①, the collected host eggs are placed in the reactor 1 in the parasitic egg magnetization measuring device, and are stacked on the support plate 10; the artificial yolk liquid in the artificial yolk liquid storage tank 5 is transported into the reactor 1 and the host eggs are immersed, and the argon bottle 3 is used to transport high-pressure argon gas into the reactor 1. After the air pressure in the reactor 1 reaches 2-3 MPa, the support plate rotary shaft drive motor 13 is started to rotate the support plate 10 to stir the host eggs, and the process is stopped after 1-2 hours, and the transport of high-pressure argon gas is stopped, and finally the air pressure in the reactor 1 is released to normal pressure;

[0036] The artificial yolk solution is prepared by mixing glucose, tussah egg yolk, carboxylated ferric oxide nanoparticles and purified water;

[0037] ②, the artificial yolk liquid in the reactor 1 is drained, and the purified water in the pure water storage tank 4 is used to rinse the host eggs through the spray head 9, and the purified water in the reactor 1 is drained after rinsing;

[0038] ③, using the hot air blower 2 to dry the host eggs in the reaction kettle 1, the drying temperature is 20-25°C;

[0039] ④, injecting the purified water in the purified water storage tank 4 into the reactor 1 through the spray head 9 until the host eggs are submerged and the liquid level is higher than the discharge port 8, and then adding sucrose to the purified water in the reactor 1 to obtain a sucrose solution;

[0040] The mass fraction of sucrose in the sucrose solution is 10%;

[0041] 5. Start the support plate rotary shaft drive motor 13 to rotate the support plate 10 to stir the host eggs, and at the same time turn on the power of the electromagnet 12. Under the magnetic force, the host eggs floating in the sucrose solution are stratified, and some host eggs move to the support plate 10 to the lower layer of the sucrose solution, and some host eggs float on the upper layer of the sucrose solution. The host eggs in the lower layer are parasitic eggs.

[0042] The parasitic egg magnetization measuring device can measure the parasitic eggs that have been parasitized by the magnetization measuring method and separate the parasitic eggs from the unparasitized host eggs. It can effectively control the number of parasitism, avoid the occurrence of repeated parasitism, and achieve effective control of over-parasitism, thereby ensuring the improvement of the consistency of individual development in the parasitic eggs; it is convenient for artificial intervention in the number of parasitism, and the measuring method is highly reliable and efficient, and there is no time lag. The artificial yolk liquid used in the use of the device contains glucose, tussah egg yolk, and carboxylated ferric oxide nanoparticles. The tussah egg yolk is used to supplement the yolk of the host eggs. The carboxylated ferric oxide nanoparticles are magnetic particles with good biocompatibility. The carboxylated ferric oxide nanoparticles are used to make the host eggs magnetic, and then the magnetic field is used to measure and separate the parasitized host eggs. The artificial yolk liquid contains glucose and tussah egg yolk, which is beneficial to the individual development of Trichogramma, and can significantly improve the body length and uniformity, emergence rate, lifespan, etc. of Trichogramma.

[0043] Specific implementation method six: This implementation method is different from specific implementation method five in that the mass fraction of glucose in the artificial yolk solution is 4-5%, the mass fraction of carboxylated ferric oxide nanoparticles is 0.5-1%, and the mass fraction of tussah egg yolk is 70-75%.

[0044] Specific implementation method seven: This implementation method is different from specific implementation methods five or six in that the yolk of the tussah egg is obtained by cutting open the abdomen of the female tussah moth to take out the egg and remove the egg shell.

[0045] Specific implementation eight: This implementation differs from any one of specific implementations five to seven in that: the particle size of the carboxylated ferric oxide nanoparticles is 10-50 nm.

[0046] Specific embodiment nine: This embodiment differs from any one of specific embodiments five to eight in that: after step 2 determines that parasitism has occurred, the non-parasitized host eggs and parasitized eggs are separated using a parasitized egg magnetization measuring device; the parasitized eggs are cultured and prepared into bee cards for preservation; the non-parasitized host eggs are repeated with steps 1 and 2 for inoculation and measurement until all non-parasitized host eggs have been inoculated with bees and the magnetization measurement of parasitic eggs during the artificial breeding of Trichogrammatids is completed.

[0047] Specific embodiment ten: This embodiment differs from any one of specific embodiments five to nine in that: the method of separating non-parasitized host eggs and parasitic eggs using a parasitic egg magnetization measuring device is as follows: the host eggs in the upper and lower layers are completely separated by controlling the power supply voltage of the electromagnet 12; then the discharge port 8 is opened to discharge the host eggs in the upper layer of the sucrose solution, and then the power supply of the electromagnet 12 is turned off, and the host eggs located on the support plate 10 are parasitic eggs that have completed parasitism; the support plate 10 is lifted by the support plate lifting drive motor 17 and the parasitic eggs are rinsed with purified water in the pure water storage tank 4 through the spray head 9, and finally discharged through the discharge port 8.

[0048] Example 1

[0049] The magnetization determination method of parasitic eggs in the artificial breeding process of Trichogramma in this embodiment is carried out according to the following steps:

[0050] Step 1, placing pine caterpillar trichogrammatid bee species and host eggs in a culture room for bee inoculation; the number ratio of trichogrammatid bee species to host eggs is 1:60; the bee inoculation time is 5 hours, wherein, before the 4th hour, the temperature in the culture room is controlled at 12°C and the humidity is 70%; from the 4th hour to the 5th hour, the temperature in the culture room is controlled at 25°C and the humidity is 75%; the host eggs are tussah eggs; the pine caterpillar trichogrammatid bee species tussah is used as the host, and the temperature is 25°C indoors, L:D=14h:10h (L is light, D is dark, L:D=14h:10h means: the light time is 14h and the dark time is 10h within 24 hours.) Reproduction for 10 generations;

[0051] Step 2, separating the trichogrammatid bee species and the host eggs, collecting the host eggs, and performing a magnetization measurement of the host eggs using a parasitic egg magnetization measurement device, and determining the host eggs that have been parasitized in the collected host eggs, namely the parasitic eggs;

[0052] The method for measuring the magnetization of host eggs by using the parasitic egg magnetization measuring device is as follows:

[0053] ①, the collected host eggs are placed in the reactor 1 in the parasitic egg magnetization measuring device, and are stacked on the support plate 10; the artificial yolk liquid in the artificial yolk liquid storage tank 5 is transported into the reactor 1 and the host eggs are immersed, and the argon bottle 3 is used to transport high-pressure argon gas into the reactor 1. After the air pressure in the reactor 1 reaches 2.5MPa, the support plate rotary shaft drive motor 13 is started to rotate the support plate 10 to stir the host eggs, and the process is stopped after 2h, and the transport of high-pressure argon gas is stopped, and finally the air pressure in the reactor 1 is released to normal pressure;

[0054] The artificial yolk solution is prepared by mixing glucose, tussah egg yolk, carboxylated ferric oxide nanoparticles and purified water;

[0055] The mass fraction of glucose in the artificial yolk liquid is 4.5%, the mass fraction of carboxylated ferric oxide nanoparticles is 0.8%, and the mass fraction of tussah egg yolk is 70%;

[0056] The yolk of the tussah silkworm egg is obtained by dissecting the female tussah silkworm moth to take out the egg and remove the egg shell;

[0057] The particle size of the carboxylated ferric oxide nanoparticles is 10-20 nm;

[0058] ②, the artificial yolk liquid in the reactor 1 is drained, and the purified water in the pure water storage tank 4 is used to rinse the host eggs through the spray head 9, and the purified water in the reactor 1 is drained after rinsing;

[0059] ③, using the hot air blower 2 to dry the host eggs in the reaction kettle 1, the drying temperature is 25°C;

[0060] ④, injecting the purified water in the purified water storage tank 4 into the reactor 1 through the spray head 9 until the host eggs are submerged and the liquid level is higher than the discharge port 8, and then adding sucrose to the purified water in the reactor 1 to obtain a sucrose solution;

[0061] The mass fraction of sucrose in the sucrose solution is 10%;

[0062] 5. Start the support plate rotary shaft drive motor 13 to rotate the support plate 10 to stir the host eggs, and at the same time turn on the power of the electromagnet 12, so that the host eggs floating in the sucrose solution are stratified under the magnetic force, and some host eggs move to the lower layer of the sucrose solution toward the support plate 10, and some host eggs float on the upper layer of the sucrose solution. The host eggs in the lower layer are parasitic eggs;

[0063] After parasitism is detected in step 2, the parasitoid egg magnetization measuring device is used to separate the unparasitized host eggs from the parasitoid eggs; the parasitoid eggs are cultured and prepared into bee cards for storage; the unparasitized host eggs are repeated in steps 1 and 2 for bee connection and measurement until all the unparasitized host eggs are connected with bees, and the magnetization measurement of the parasitoid eggs in the artificial breeding process of trichogrammatids is completed; the method of separating the unparasitized host eggs from the parasitoid eggs using the parasitoid egg magnetization measuring device is as follows: the host eggs in the upper layer and the lower layer are completely separated by controlling the power supply voltage of the electromagnet 12; then the discharge port 8 is opened to discharge the host eggs in the upper layer of the sucrose solution, and then the power supply of the electromagnet 12 is turned off, and the host eggs located on the support plate 10 are parasitoid eggs that have completed parasitism; the support plate 10 is lifted by the support plate lifting drive motor 17, and the parasitic eggs are rinsed by the purified water in the pure water storage tank 4 through the spray head 9, and finally discharged through the discharge port 8;

[0064] The parasitic egg magnetization measuring device is composed of a reactor 1, a hot air blower 2, an argon gas bottle 3, a pure water storage tank 4, an artificial yolk liquid storage tank 5, a support plate drive control box 6 and a wastewater storage tank 7; a discharge port 8 is provided on the upper part of the side wall of the reactor 1, and a valve is provided on the discharge port 8; a spray head 9 is provided on the top of the reactor 1, and the spray head 9 is connected to the water outlet pipe of the pure water storage tank 4 arranged outside the reactor 1; a hot air pipe is provided on the top of the reactor 1, and the hot air pipe is connected to the exhaust pipe of the hot air blower 2 arranged outside the reactor 1; a high-pressure gas inlet is provided on the side wall of the reactor 1, and the high-pressure gas inlet is connected to the outlet pipe of the argon gas bottle 3 arranged outside the reactor 1; an artificial yolk liquid inlet, an artificial yolk liquid inlet, and an argon gas bottle 3 arranged outside the reactor 1 are provided at the bottom of the reactor 1. The reactor 1 is provided with an outlet and a wastewater outlet, the artificial yolk liquid inlet is connected to the artificial yolk liquid conveying pipe of the artificial yolk liquid storage tank 5 arranged outside the reactor 1, the artificial yolk liquid outlet is connected to the artificial yolk liquid recovery pipe of the artificial yolk liquid storage tank 5, and the wastewater outlet is connected to the water inlet pipe of the wastewater storage tank 7 arranged outside the reactor 1; a support plate 10 is arranged in the reactor 1, the support plate 10 is a circular plate, the support plate 10 is inclined, the support plate 10 is a porous plate, the edge of the support plate 10 is in close contact with the inner wall of the reactor 1, and a vertical support plate rotating shaft 11 is arranged at the center of the lower surface of the support plate 10; the support plate drive control box 6 is arranged at the bottom of the reactor 1 and the support plate drive control box 6 is sealed and connected to the bottom of the reactor 1; the support plate drive control box 6 is provided with The pallet rotary shaft driving motor 13 and the pallet lifting driving motor 17, the lower end of the pallet rotary shaft 11 passes through the rotary shaft through hole at the center of the bottom plate of the reactor 1 and is connected to the power output shaft of the pallet rotary shaft driving motor 13, and a mechanical seal is arranged between the pallet rotary shaft 11 and the rotary shaft through hole at the center of the bottom plate of the reactor 1; two guide plates 16 are symmetrically arranged on both sides of the pallet rotary shaft driving motor 13, and the two ends of the guide plate 16 are respectively connected to the bottom of the reactor 1 and the bottom of the pallet drive control box 6, and a vertical T-shaped slide groove 18 is arranged on the guide plate 16; two horizontal connecting plates 14 are symmetrically arranged on the shell of the pallet rotary shaft driving motor 13, and one end of the connecting plate 14 is fixedly connected to the shell of the pallet rotary shaft driving motor 13 , a slider is fixedly connected to the other end of the shell of the connecting plate 14, and the slider is arranged in the T-shaped slide groove 18; a vertical threaded through hole is arranged on the connecting plate 14, and the pallet lifting drive motor 17 is fixed on the bottom plate inside the pallet drive control box 6, and a vertical screw 15 is connected to the power output shaft of the pallet lifting drive motor 17, and the screw 15 is arranged in the vertical thread of the connecting plate 14; the electromagnet 12 is arranged on the bottom plate inside the reactor 1, and the surface of the electromagnet 12 is sealed and coated with epoxy resin; valves are arranged on the water outlet pipe of the pure water storage tank 4, the exhaust pipe of the hot air blower 2, the air outlet pipe of the argon gas bottle 3, the artificial yolk liquid delivery pipe of the artificial yolk liquid storage tank 5, the artificial yolk liquid recovery pipe of the artificial yolk liquid storage tank 5 and the water inlet pipe of the wastewater storage tank 7.

[0065] Example 2: This example is different from Example 1 in that the mass fraction of glucose in the artificial yolk solution is 5%, the mass fraction of carboxylated ferric oxide nanoparticles is 0.5%, and the mass fraction of tussah egg yolk is 75%. Other steps and processes are the same as in Example 1.

[0066] Example 3: This example is different from Example 1 in that the mass fraction of glucose in the artificial yolk solution is 5%, the mass fraction of carboxylated ferric oxide nanoparticles is 0.5%, and the mass fraction of tussah egg yolk is 70%. Other steps and processes are the same as in Example 1.

[0067] Comparative Example:

[0068] Step 1, placing pine caterpillar trichogrammatid bee species and host eggs in a culture room for bee inoculation; the number ratio of trichogrammatid bee species to host eggs is 1:60; the bee inoculation time is 5 hours, the temperature in the culture room is controlled at 25°C, and the humidity is 75%; the host eggs are tussah eggs; pine caterpillar trichogrammatid bee species tussah as the host in the room (25°C, L:D=14h:10h, 10 generations of reproduction); the number of parasitism is counted after 24 hours, and the body length and uniformity, emergence rate, lifespan and the like are tested 48 hours later.

[0069]

[0070] Table 1 is the data of the offspring bee emergence rate in the embodiment, and Table 3 is the data of the percentage of body length of the offspring bee (female bee) in the embodiment, which is tested according to the prior art. Table 2 is the statistics of the number of parasitism of the separated parasitic eggs. The statistical method is to take 1,000 parasitic eggs, count the number of ovulations of the bee species in each parasitic egg, and find the parasitic eggs with the least number of ovulations among the 100 eggs, calculate the average number of ovulations of the 100 rows, and the average number of ovulations of the bee species in each parasitic egg / the average number of ovulations of the 100 rows is the reference value of the number of ovulations; the reference value of the number of ovulations <1.5 is 1 ovulation (1 parasitism), 1.6≤the reference value of the number of ovulations <2.5 is 2 ovulations, and the reference value of the number of ovulations ≥2.6 is 3 ovulations or more.

[0071] The female ratio of the offspring bees obtained by statistics (thousands of bees) in Examples 1 to 3 was 49-50%, while the female ratio of the control group was only 44-47%. The average life span of the offspring bees obtained in Example 1 was increased by 0.9 days compared with the control group. Therefore, the present invention can significantly improve the body length and uniformity, emergence rate, life span, etc. of the trichogrammatid bees, and is significantly better than the control group, and significantly reduces the over-parasitism ratio, thereby achieving effective control of over-parasitism.

Claims

1. A parasitic egg magnetization measuring device, characterized in that: The parasitic egg magnetization measuring device comprises a reaction kettle (1), a hot air blower (2), an argon gas bottle (3), a pure water storage tank (4), an artificial yolk liquid storage tank (5), a support plate drive control box (6) and a wastewater storage tank (7); a discharge port (8) is provided on the upper part of the side wall of the reaction kettle (1), and a valve is provided on the discharge port (8); a spray head (9) is provided on the top of the reaction kettle (1), and the spray head (9) is connected to the water outlet pipe of the pure water storage tank (4) arranged outside the reaction kettle (1); a hot air pipe is provided on the top of the reaction kettle (1), and the hot air pipe is connected to the exhaust pipe of the hot air blower (2) arranged outside the reaction kettle (1); a high-pressure gas inlet is provided on the side wall of the reaction kettle (1), and the high-pressure gas inlet is connected to the outlet pipe of the hot air blower (2) arranged outside the reaction kettle (1); ) is connected to the outlet pipe of the argon gas bottle (3) outside the reactor (1); the bottom of the reactor (1) is provided with an artificial yolk liquid inlet, an artificial yolk liquid outlet and a wastewater outlet, the artificial yolk liquid inlet is connected to the artificial yolk liquid delivery pipe of the artificial yolk liquid storage tank (5) outside the reactor (1), the artificial yolk liquid outlet is connected to the artificial yolk liquid recovery pipe of the artificial yolk liquid storage tank (5), and the wastewater outlet is connected to the water inlet pipe of the wastewater storage tank (7) outside the reactor (1); a support plate (10) is provided in the reactor (1), the support plate (10) is a circular plate, the support plate (10) is inclined, the support plate (10) is a porous plate, the edge of the support plate (10) is in close contact with the inner wall of the reactor (1), and the lower surface of the support plate (10) is A vertical pallet rotating shaft (11) is arranged at the center of the surface; a pallet driving control box (6) is arranged at the bottom of the reactor (1) and the pallet driving control box (6) is sealedly connected to the bottom of the reactor (1); a pallet rotating shaft driving motor (13) and a pallet lifting driving motor (17) are arranged in the pallet driving control box (6); the lower end of the pallet rotating shaft (11) passes through the rotating shaft through hole at the center of the bottom plate of the reactor (1) and is connected to the power output shaft of the pallet rotating shaft driving motor (13); two guide plates (16) are symmetrically arranged on both sides of the pallet rotating shaft driving motor (13); the two ends of the guide plates (16) are respectively connected to the bottom of the reactor (1) and the bottom of the pallet driving control box (6); the guide plates (16) A vertical T-shaped slide groove (18) is arranged on the top; two horizontal connecting plates (14) are symmetrically arranged on the shell of the pallet rotary shaft driving motor (13), one end of the connecting plate (14) is fixedly connected to the shell of the pallet rotary shaft driving motor (13), and the other end of the connecting plate (14) is fixedly connected to a sliding block, and the sliding block is arranged in the T-shaped slide groove (18); a vertical threaded through hole is arranged on the connecting plate (14), the pallet lifting driving motor (17) is fixed on the bottom plate inside the pallet driving control box (6), and a vertical screw rod (15) is connected to the power output shaft of the pallet lifting driving motor (17), and the screw rod (15) is arranged in the vertical thread of the connecting plate (14); the electromagnet (12) is arranged on the bottom plate inside the reaction kettle (1); The artificial yolk solution is prepared by mixing glucose, tussah egg yolk, carboxylated ferric oxide nanoparticles and purified water.

2. The parasitic egg magnetization measuring device according to claim 1, characterized in that: Valves are provided on the water outlet pipe of the pure water storage tank (4), the exhaust pipe of the hot air blower (2), the air outlet pipe of the argon gas bottle (3), the artificial yolk liquid delivery pipe of the artificial yolk liquid storage tank (5), the artificial yolk liquid recovery pipe of the artificial yolk liquid storage tank (5) and the water inlet pipe of the wastewater storage tank (7).

3. The parasitic egg magnetization measuring device according to claim 1, characterized in that: The surface of the electromagnet (12) is sealed and coated with epoxy resin.

4. The parasitic egg magnetization measuring device according to claim 1, characterized in that: A mechanical seal is provided between the support plate rotating shaft (11) and the rotating shaft through hole at the center of the bottom plate of the reaction kettle (1).

5. A method for measuring the magnetization of parasitic eggs during artificial breeding of trichogrammatids using the parasitic egg magnetization measuring device as claimed in claim 1, characterized in that: The method proceeds as follows: Step 1, placing trichogrammatid bee species and host eggs in a culture room for bee inoculation; the number ratio of trichogrammatid bee species to host eggs is 1:50-60; the bee inoculation time is 5 hours, wherein, before the 4th hour, the temperature in the culture room is controlled at 10-15° C., and the humidity is 65-75%; from the 4th hour to the 5th hour, the temperature in the culture room is controlled at 23-25° C., and the humidity is 65-75%; Step 2, separating the trichogrammatid bee species and the host eggs, collecting the host eggs, and performing a magnetization measurement of the host eggs using a parasitic egg magnetization measurement device, and determining the host eggs that have been parasitized in the collected host eggs, namely the parasitic eggs; The method for measuring the magnetization of host eggs by using the parasitic egg magnetization measuring device is as follows: ①, the collected host eggs are placed in a reactor (1) in a parasitic egg magnetization measuring device and stacked on a support plate (10); the artificial yolk liquid in an artificial yolk liquid storage tank (5) is transported into the reactor (1) and the host eggs are immersed, and at the same time, an argon bottle (3) is used to transport high-pressure argon gas into the reactor (1). After the air pressure in the reactor (1) reaches 2-3 MPa, the support plate rotary shaft drive motor (13) is started to rotate the support plate (10) to stir the host eggs, and the process is stopped after 1-2 hours, and the transport of high-pressure argon gas is stopped, and finally the air pressure in the reactor (1) is released to normal pressure; The artificial yolk solution is prepared by mixing glucose, tussah egg yolk, carboxylated ferric oxide nanoparticles and purified water; ②, draining the artificial yolk liquid from the reaction kettle (1), and using the purified water in the pure water storage tank (4) to rinse the host eggs through the spray head (9), and after rinsing, draining the purified water from the reaction kettle (1); ③, using a hot air blower (2) to dry the host eggs in the reaction kettle (1), the drying temperature being 20-25° C.; ④, the purified water in the purified water storage tank (4) is then injected into the reaction kettle (1) through the spray head (9) until the host eggs are submerged and the liquid level is higher than the discharge port (8), and then sucrose is added to the purified water in the reaction kettle (1) to obtain a sucrose solution; The mass fraction of sucrose in the sucrose solution is 10%; 5. Start the support plate rotary shaft drive motor (13) to rotate the support plate (10) to stir the host eggs, and at the same time turn on the power supply of the electromagnet (12). Under the magnetic force, the host eggs floating in the sucrose solution are stratified, and some host eggs move to the support plate (10) to the lower layer of the sucrose solution, and some host eggs float on the upper layer of the sucrose solution. The host eggs in the lower layer are parasitic eggs.

6. The method for determining the magnetization of parasitic eggs during artificial breeding of Trichogramma according to claim 5, characterized in that: The mass fraction of glucose in the artificial yolk liquid is 4-5%, the mass fraction of carboxylated ferric oxide nanoparticles is 0.5-1%, and the mass fraction of tussah egg yolk is 70-75%.

7. The method for determining the magnetization of parasitic eggs during artificial breeding of Trichogramma according to claim 5, characterized in that: The yolk of the tussah silkworm egg is obtained by cutting open the abdomen of the female tussah silkworm moth to take out the egg and remove the egg shell.

8. The method for determining the magnetization of parasitic eggs during artificial breeding of Trichogramma according to claim 5, characterized in that: The particle size of the carboxylated ferric oxide nanoparticles is 10 to 50 nm.

9. The method for magnetization determination of parasitic eggs in the artificial breeding process of Trichogramma according to claim 5, characterized in that: After parasitism is detected in step 2, non-parasitized host eggs and parasitized eggs are separated using a parasitized egg magnetization measuring device; the parasitized eggs are cultured and prepared into bee cards for storage; and steps 1 and 2 are repeated for the non-parasitized host eggs to bee-inoculated and measured until all the non-parasitized host eggs are inoculated with bees and the magnetization measurement of parasitized eggs during the artificial breeding of trichogrammatids is completed.

10. The method for determining the magnetization of parasitic eggs during artificial breeding of Trichogramma according to claim 9, characterized in that: The method for separating unparasitized host eggs and parasitized eggs by using a parasitized egg magnetization measuring device comprises: completely separating the host eggs in the upper layer and the lower layer by controlling the power supply voltage of an electromagnet (12); then opening a discharge port (8) to discharge the host eggs in the upper layer of a sucrose solution; then turning off the power supply of the electromagnet (12); the host eggs on the support plate (10) are parasitized eggs; lifting the support plate (10) by a support plate lifting drive motor (17) and washing the parasitic eggs by using purified water in a pure water storage tank (4) through a spray head (9); and finally discharging the parasitized eggs through the discharge port (8).

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