POCT blood cell analyzer, kit, detection assembly and detection method

Abstract

The invention discloses a POCT hematology analyzer, a kit, a single-channel detection assembly and a detection method.The single-channel detection assembly comprises a detection piece, a first electrode and a second electrode, the detection piece is provided with a micropore, and a sample analyzer sucks a first to-be-detected sample through the micropore; a first detection item is detected on the first to-be-detected sample through the first electrode and the second electrode; when the first detection item of the first to-be-detected sample is completed, the to-be-detected sample and the remaining first to-be-detected sample are mixed to obtain a second to-be-detected sample, or the remaining first to-be-detected sample is discharged, and the second to-be-detected sample is added into the detection piece; the sample analyzer sucks a second to-be-detected sample through the micropore, and detects a second detection item on the second to-be-detected sample through the first electrode and the second electrode. The second detection item of the second to-be-detected sample is detected through the same detection piece, the cost of the detection assembly is reduced, and the cost of consumables is reduced.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a POCT (Point-of-care testing) blood cell analyzer, a test kit, a single-channel detection component and a detection method. Background Art

[0002] Currently, disposable blood cell test kits generally perform one counting test on the sample; if the sample needs to be counted for red blood cells and platelets, or red blood cells and white blood cells, two sets of detection channels need to be set up on the test kit, resulting in high consumables costs. Summary of the invention

[0003] In order to solve the above problems, the present application provides a single-channel detection component, which is applied to a sample analyzer. The single-channel detection component includes:

[0004] A detection member, used for accommodating a first sample to be detected, wherein the detection member is provided with micropores;

[0005] A first electrode and a second electrode, wherein the first electrode and the second electrode are respectively located on two sides of the micropore;

[0006] The detection component is installed in the sample analyzer, and the sample analyzer sucks the first sample to be detected through the micropore, and obtains electrical signals through the first electrode and the second electrode to count the first sample to be detected, so as to detect a first detection item on the first sample to be detected;

[0007] When the first test item of the first sample to be tested is completed, the test piece is added to the sample to be tested, and is used to mix with the remaining first sample to be tested in the test piece to obtain a second sample to be tested, and the dilution factor of the sample to be tested is less than or equal to the dilution factor of the first sample to be tested; or, the remaining first sample to be tested is discharged, and the test piece is added to the second sample to be tested;

[0008] The sample analyzer draws the second sample to be detected through the micropore, and obtains electrical signals through the first electrode and the second electrode to count the second sample to be detected, so as to detect a second detection item on the second sample to be detected.

[0009] Wherein, the first sample to be detected includes red blood cells or platelets, and the second sample to be detected includes white blood cells;

[0010] When the first test item of the first sample to be tested is completed and the liquid level of the first sample to be tested in the testing member is higher than the preset liquid level, the sample analyzer continues to draw the first sample to be tested through the micropore;

[0011] Alternatively, the detection component includes a pipette. When the first detection item of the first sample to be detected is completed and the liquid level of the first sample to be detected in the detection piece is higher than the preset liquid level, the pipette is used to aspirate the first sample to be detected in the detection piece so that the liquid level of the first sample to be detected is at or below the preset liquid level.

[0012] The preset liquid level is located at or below the plane where the central axis of the micropore is located, and the difference between the initial liquid level of the first sample to be detected and the preset liquid level ranges from 10 μL to 5000 μL.

[0013] In order to solve the above problems, the present application provides a detection method, which is applied to the above single-channel detection component, and the detection method includes:

[0014] Adding a first sample to be tested into the testing component, and installing the testing component on a sample analyzer;

[0015] Controlling the sample analyzer to draw the first sample to be detected through the micropore, and obtaining electrical signals through the first electrode and the second electrode to count the first sample to be detected, so as to detect a first detection item on the first sample to be detected;

[0016] When the first test item of the first sample to be tested is completed, the sample to be tested is added to the testing member to be mixed with the remaining first sample to be tested in the testing member to obtain a second sample to be tested, and the dilution factor of the sample to be tested is less than or equal to the dilution factor of the first sample to be tested; or, the remaining first sample to be tested is discharged, and the second sample to be tested is added to the testing member;

[0017] The sample analyzer is controlled to draw the second sample to be detected through the micropore, and the electrical signal is obtained through the first electrode and the second electrode to count the second sample to be detected, so as to detect a second detection item on the second sample to be detected.

[0018] Wherein, the first sample to be detected includes red blood cells or platelets, the second sample to be detected includes white blood cells, and the detection method further includes:

[0019] In response to completing the first test item of the first sample to be tested, and the liquid level of the first sample to be tested in the testing member is higher than a preset liquid level, controlling the sample analyzer to continue to draw the first sample to be tested through the micropores so that the liquid level of the first sample to be tested is at or below the preset liquid level;

[0020] Wherein, the preset liquid level is located at or below the plane where the central axis of the micropore is located.

[0021] Wherein, the single-channel detection component includes a pipette, and the detection method further includes:

[0022] In response to completing the first test item of the first sample to be tested, and the liquid level of the first sample to be tested in the testing member is higher than a preset liquid level, controlling the pipette to aspirate the first sample to be tested in the testing member so that the liquid level of the first sample to be tested is lower than the preset liquid level;

[0023] Wherein, the preset liquid level is lower than the plane where the central axis of the microhole is located and is close to the bottom of the detection element.

[0024] Wherein, the difference between the initial liquid level of the first sample to be detected and the preset liquid level ranges from 10 μL to 5000 μL.

[0025] Wherein, the sample analyzer includes a light source and a receiver, and the detection method includes:

[0026] In response to completing the second inspection item of the second sample to be inspected, controlling the light source to generate light so that the light irradiates the second sample to be inspected in the inspection member;

[0027] The light passing through the second sample to be detected is received by the receiver to detect a third detection item of the second sample to be detected.

[0028] In order to solve the above problems, the present application provides a test kit, including a box body and the above-mentioned single-channel detection component, wherein the single-channel detection component is located on the box body.

[0029] To solve the above problems, the present application provides a POCT blood cell analyzer, comprising a detection seat, a pressure source and a constant current source, wherein the detection seat is used to place the above-mentioned test kit, and the pressure source is used to apply pressure to the detection piece of the test kit so that the first sample to be detected or the second sample to be detected in the detection piece passes through the micropores of the detection piece; the constant current source is used to power the first electrode and the second electrode of the detection component.

[0030] The single-channel detection component of the present application includes a detection member, a first electrode and a second electrode. The detection member is provided with a micropore, and the first electrode and the second electrode are respectively located on both sides of the micropore. The detection member is installed on a sample analyzer. The sample analyzer sucks the first sample to be detected through the micropore, and counts the first sample to be detected through the first electrode and the second electrode, so as to detect the first detection item of the first sample to be detected; when the first detection item of the first sample to be detected is completed, the detection member adds the sample to be detected, which is used to mix with the remaining first sample to be detected in the detection member to obtain the second sample to be detected, or discharge the remaining first sample to be detected, and the detection member adds the second sample to be detected; the sample analyzer sucks the second sample to be detected through the micropore, and counts the second sample to be detected through the first electrode and the second electrode, so as to detect the second detection item of the second sample to be detected. Wherein, the detection member is installed on the sample analyzer, and the sample analyzer detects the first detection item of the first sample to be detected through the detection member, and detects the second detection item of the second sample to be detected through the same detection member, which can realize the detection of the first detection item and the second detection item through the same detection member, thereby reducing the cost of the detection member and the cost of consumables. In addition, when the first test item of the first sample to be tested is completed, the test piece is added to the sample to be tested or the second sample to be tested, and there is no need to clean the test piece, which saves the cleaning system, further reduces costs and improves detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:

[0032] Figure 1 It is a structural schematic diagram of an embodiment of a single-channel detection component of the present application;

[0033] Figure 2 It is a structural schematic diagram of another embodiment of the single-channel detection component of the present application;

[0034] Figure 3 It is a schematic diagram of a flow chart of an embodiment of a detection method of the present application;

[0035] Figure 4 It is a flow chart of another embodiment of the detection method of the present application.

[0036] Reference numerals: single-channel detection component 10 , detection member 11 , micropore 111 , first electrode 12 , second electrode 13 , pipette 14 , pressure source 21 , constant current source 22 , preset liquid level H1 , light source 23 , receiver 24 . DETAILED DESCRIPTION

[0037] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. It is to be understood that the specific embodiments described herein are only used to explain the present application, rather than to limit the present application. It should also be noted that, for ease of description, only some structures related to the present application are shown in the accompanying drawings, rather than all structures. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0038] The terms "first", "second", etc. in this application are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "provided with" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.

[0039] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "set", "connected", and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or connected through an intermediate medium. For ordinary technicians in this field, if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of this application, the directional indications are only used to explain the relative position relationship, movement, etc. between the components in a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0040] Reference to "embodiments" in this application means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0041] See also Figure 1 As shown, Figure 1The single-channel detection component 10 of this embodiment is applied to a sample analyzer, which is a commonly used medical device for testing samples and detecting various immune indicators in samples. For example, the sample analyzer can be a POCT blood cell analyzer.

[0042] According to some embodiments of the present application, the single-channel detection assembly 10 includes a detection member 11, a first electrode 12 and a second electrode 13. The detection member 11 is used to accommodate a first sample to be detected, and the detection member 11 can be called a detection cup, a reaction cup or a reaction pool.

[0043] Optionally, the first sample to be detected may be composed of a first reagent and a first cell component test liquid, the first reagent may be a diluent, and the first cell component test liquid may be a red blood cell test liquid or a platelet test liquid, that is, the first sample to be detected includes red blood cells or platelets. In other embodiments, the first sample to be detected may be a whole blood sample or a serum sample, etc.

[0044] The detection element 11 is provided with micropores 111, and the micropores 111 are used to allow blood cells in the first sample to be detected to pass through one by one, that is, the diameter of the micropores 111 can be set correspondingly according to the different particle sizes of the blood cells.

[0045] The first electrode 12 and the second electrode 13 are respectively located on both sides of the micropore 111, for example, the first electrode 12 is arranged on the side of the detection member 11 away from the micropore 111, and the second electrode 13 is arranged on the side of the micropore 111 away from the first electrode 12. Optionally, one end of the first electrode 12 is located inside the detection member 11, and the other end of the first electrode 12 is located outside the detection member 11, wherein the detection member 11 can be called a front cell, and the first electrode 12 can be called a front cell electrode. Optionally, the second electrode 13 is located outside the detection member 11, and the second electrode 13 is arranged close to the micropore 111, wherein the second electrode 13 can be called a back cell electrode, and the accommodation space where the second electrode 13 is located can be called a back cell.

[0046] When the single-channel detection component 10 is installed in a sample analyzer, the sample analyzer draws the first sample to be detected through the micropores 111, so that the blood cells in the first sample to be detected pass through the micropores 111 one by one; the sample analyzer obtains electrical signals through the first electrode 12 and the second electrode 13 to count the first sample to be detected, so as to detect the first detection item of the first sample to be detected. For example, the first detection item includes but is not limited to a red blood cell detection item or a platelet detection item.

[0047] Optionally, the sample analyzer includes a pressure source 21 and a constant current source 22. The single-channel detection component 10 is installed on the sample analyzer. The pressure source 21 is connected to the back pool to provide pressure to draw the first sample to be detected through the micropore 111; the constant current source 22 is electrically connected to the first electrode 12 and the second electrode 13, so that the sample analyzer counts the first sample to be detected and detects the first detection item on the first sample to be detected.

[0048] During the process of the sample analyzer sucking the first sample to be detected through the micropore 111, the liquid level of the first sample to be detected in the detection element 11 gradually decreases. When the liquid level of the first sample to be detected is at or below the preset liquid level H1, the sample analyzer completes the detection of the first detection item on the first sample to be detected, that is, completes the first detection item of the first detection sample, and at this time, there is a remaining first sample to be detected in the detection element 11.

[0049] When the first test item of the first test sample is completed, the test piece 11 is added to the sample to be tested, and the sample to be tested is used to mix with the remaining first sample to be tested in the test piece 11 to obtain a second sample to be tested, wherein the dilution factor of the sample to be tested is less than or equal to the dilution factor of the first sample to be tested.

[0050] When the liquid level of the first sample to be detected is at or below the preset liquid level H1, the detection element 11 is added to the sample to be detected, wherein the dilution factor of the sample to be detected is less than or equal to the dilution factor of the first sample to be detected. For example, the dilution factor of the first sample to be detected may range from 1000 to 50000, and the dilution factor of the sample to be detected may range from 0 to 1000; that is, the dilution factor of the first sample to be detected may be 1000, and the dilution factor of the sample to be detected may be 1000; or, the dilution factor of the first sample to be detected may be 10000, and the dilution factor of the sample to be detected may be 100.

[0051] When the detection element 11 is added to the sample to be detected, the sample to be detected is mixed with the remaining first sample to be detected to obtain a second sample to be detected. For example, the sample to be detected can be a second cell component preparation liquid, and the second cell component preparation liquid is mixed with the remaining first sample to be detected to obtain a second cell component test liquid, and the second sample to be detected includes but is not limited to white blood cells.

[0052] Alternatively, when the first test item of the first test sample is completed, the remaining first test sample is discharged, and the second test sample is added to the test piece 11. For example, the sample analyzer sucks out the remaining first test sample in the test piece 11.

[0053] When the detection member 11 is used to accommodate the second sample to be detected, the single-channel detection assembly 10 is installed in the sample analyzer, and the sample analyzer draws the second sample to be detected through the micropore 111, and counts the second sample to be detected through the first electrode 12 and the second electrode 13, so as to detect the second detection item of the second sample to be detected. For example, the second detection item includes but is not limited to the white blood cell detection item.

[0054] The single-channel detection component 10 of the present embodiment includes a detection member 11, a first electrode 12 and a second electrode 13. The detection member 11 is provided with a micropore 111. The first electrode 12 and the second electrode 13 are respectively located on both sides of the micropore 111. The single-channel detection component 10 is installed on a sample analyzer. The sample analyzer draws a first sample to be detected through the micropore 111, and counts the first sample to be detected through the first electrode 12 and the second electrode 13, so as to detect a first detection item for the first sample to be detected; when the first detection item of the first sample to be detected is completed, the detection member 11 adds the sample to be detected to be mixed with the remaining first sample to be detected in the detection member 11 to obtain a second sample to be detected, or the remaining first sample to be detected is discharged, and the detection member 11 adds the second sample to be detected; the sample analyzer draws a second sample to be detected through the micropore 111, and counts the second sample to be detected through the first electrode 12 and the second electrode 13, so as to detect a second detection item for the second sample to be detected. Among them, the single-channel detection component 10 is installed in the sample analyzer, and the sample analyzer detects the first detection item on the first sample to be detected through the detection component 11, and detects the second detection item on the second sample to be detected through the same detection component 11, which can realize the detection of the first detection item and the second detection item through the same detection component 11, reducing the cost of the single-channel detection component 10 and the cost of consumables. In addition, when the first detection item of the first sample to be detected is completed, the detection component 11 is added to the sample to be detected or the second sample to be detected, and the detection component 11 does not need to be cleaned, which saves the cleaning system, further reduces the cost, and improves the detection efficiency.

[0055] According to some embodiments of the present application, when the first test item of the first sample to be tested is completed and the liquid level of the first sample to be tested in the testing piece 11 is higher than the preset liquid level H1, the sample analyzer continues to aspirate the first sample to be tested in the testing piece 11 through the micropore 111 until the liquid level of the first sample to be tested in the testing piece 11 is at or below the preset liquid level H1.

[0056] Since the initial liquid level of the first sample to be detected in the detection element 11 is relatively high, the initial liquid level is the liquid level of the first sample to be detected when the detection element 11 completes the addition of the first sample to be detected; when the sample analyzer completes the detection of the first sample to be detected, the liquid level of the first sample to be detected in the detection element 11 is higher than the preset liquid level H1, resulting in too much first sample to be detected remaining in the detection element 11, affecting the dilution multiple of the second sample to be detected.

[0057] Therefore, in this embodiment, when the first test item of the first sample to be tested is completed and the liquid level of the first sample to be tested in the testing member 11 is higher than the preset liquid level H1, the sample analyzer continues to draw the first sample to be tested in the testing member 11 through the micropore 111 until the liquid level of the first sample to be tested in the testing member 11 is at or below the preset liquid level H1, so as to ensure that the liquid level of the first sample to be tested is at or below the preset liquid level H1, reduce the impact on the second sample to be tested, and improve the accuracy of testing the second sample to be tested.

[0058] According to some embodiments of this application, see Figure 2 As shown, Figure 2 1 is a schematic diagram of the structure of another embodiment of the detection component of the present application. The single-channel detection component 10 of the present embodiment includes a pipette 14. When the first detection item of the first sample to be detected is completed and the liquid level of the first sample to be detected in the detection member 11 is higher than the preset liquid level H1, the pipette 14 is used to suck the first sample to be detected in the detection member 11 so that the liquid level of the first sample to be detected in the detection member 11 is at or below the preset liquid level H1.

[0059] Optionally, when the first test item of the first sample to be tested is completed and the liquid level of the first sample to be tested in the testing piece 11 is higher than the preset liquid level H1, the pipette 14 is controlled to move into the testing piece 11 to aspirate the first sample to be tested in the testing piece 11 until the liquid level of the first sample to be tested in the testing piece 11 is at or below the preset liquid level H1, so as to ensure that the liquid level of the first sample to be tested is at or below the preset liquid level H1, thereby reducing the impact on the second sample to be tested and improving the accuracy of testing the second sample to be tested.

[0060] Optionally, the pipette 14 is used to aspirate all the first sample to be detected in the detection element 11 , so as to facilitate the addition of the second sample to be detected into the detection element 11 .

[0061] According to some embodiments of the present application, the preset liquid level H1 is located at or below the plane where the central axis of the micropore 111 is located. The central axis of the micropore 111 may be the center line of the micropore 111, and the preset liquid level H1 is located at or below the plane where the center line of the micropore 111 is located. Optionally, the plane where the central axis of the micropore 111 is located is a plane parallel to the liquid surface of the first sample to be detected in the detection element 11.

[0062] Among them, the difference between the initial liquid level of the first sample to be detected in the detection element 11 and the preset liquid level H1 ranges from 10μL to 5000μL, and the difference between the initial liquid level of the first sample to be detected and the preset liquid level H1 can be 10μL, 50μL, 100μL, 200μL, 300μL, 400μL, 500μL, 600μL, 700μL, 800μL, 900μL, 1000μL, 2000μL, 3000μL, 4000μL or 5000μL.

[0063] Optionally, the liquid level of the first sample to be detected in the detection element 11 is at or below the preset liquid level H1, and the total amount of the first sample to be detected in the detection element 11 is in the range of 0-10000 μL. In the case where the pipette 14 is used to aspirate the first sample to be detected in the detection element 11, the preset liquid level H1 is as close to the bottom of the detection element 11 as possible, further reducing the impact on the second sample to be detected and improving the accuracy of the detection of the second sample to be detected.

[0064] When the difference between the initial liquid level of the first sample to be detected in the detection element 11 and the preset liquid level H1 is less than 10 μL, the total amount of the first sample to be detected in the detection element 11 sucked by the sample analyzer through the micropore 111 is too small, resulting in low accuracy of the sample analyzer in detecting the first test item of the first sample to be detected. When the difference between the initial liquid level of the first sample to be detected in the detection element 11 and the preset liquid level H1 is greater than 5000 μL, the remaining first sample to be detected in the detection element 11 is too small, and it is impossible to mix with the sample to be detected to obtain the second sample to be detected.

[0065] Therefore, the difference between the initial liquid level of the first sample to be detected in the detection element 11 of the present embodiment and the preset liquid level H1 ranges from 10 μL to 5000 μL, which can ensure that the sample analyzer draws the total amount of the first sample to be detected in the detection element 11 through the micropore 111, thereby improving the accuracy of the sample analyzer in detecting the first test item of the first sample to be detected; and can also allow the remaining first sample to be detected in the detection element 11 to be mixed with the sample to be detected.

[0066] Optionally, the sample analyzer detects the liquid level of the first sample to be detected in the detection element 11 through the first electrode 12 and the second electrode 13. That is, when the sample analyzer detects a signal through the first electrode 12 and the second electrode 13, the sample analyzer determines that the liquid level of the first sample to be detected in the detection element 11 is higher than the preset liquid level H1. That is, when the sample analyzer detects a signal through the first electrode 12 and the second electrode 13, the sample analyzer determines that the liquid level of the first sample to be detected in the detection element 11 is higher than the preset liquid level H1. When the sample analyzer cannot detect a signal through the first electrode 12 and the second electrode 13, the sample analyzer determines that the liquid level of the first sample to be detected in the detection element 11 is lower than the preset liquid level H1.

[0067] The sample analyzer of this embodiment detects the liquid level of the first sample to be detected in the detection element 11 through the first electrode 12 and the second electrode 13, which is simple to implement, reduces costs, and improves the accuracy of liquid level detection.

[0068] Optionally, the sample analyzer further includes a light source 23 and a receiver 24. When the single-channel detection assembly 10 is installed on the sample analyzer and the detection element 11 stores the second sample to be detected, the light source 23 and the receiver 24 are respectively located on both sides of the detection element 11. The light source 23 is used to generate light so that the light irradiates the second sample to be detected in the detection element 11, and the receiver 24 is used to receive the light passing through the second sample to be detected. The sample analyzer detects the second sample to be detected by photoelectric colorimetry so that the sample analyzer detects the third detection item of the second sample to be detected, and the third detection item includes but is not limited to the components of the second sample to be detected.

[0069] The sample analyzer of this embodiment includes a light source 23 and a receiver 24. The sample analyzer detects the second sample to be detected by photoelectric colorimetry, so as to detect the second sample to be detected by multiple methods, thereby improving the detection capability of the sample analyzer.

[0070] According to some embodiments of this application, see Figure 3 As shown, Figure 3 1 is a flow chart of an embodiment of the detection method of the present application. The detection method of this embodiment is applied to the single-channel detection component 10 disclosed in the above embodiment, and the detection method includes the following steps:

[0071] S301: Add a first sample to be tested into the testing component 11, and install the single-channel testing component 10 on the sample analyzer.

[0072] The first sample to be detected is added to the detection element 11, and the single-channel detection component 10 is installed in the sample analyzer. In other embodiments, the first sample to be detected is added to the detection element 11 in advance, and the single-channel detection component 10 is directly installed in the sample analyzer.

[0073] Optionally, the single-channel detection component 10 is installed on a detection seat of a sample analyzer, so that the sample analyzer detects the first sample to be detected in the single-channel detection component 10 .

[0074] S302: Control the sample analyzer to draw the first sample to be detected through the micropore 111, and obtain electrical signals through the first electrode 12 and the second electrode 13 to count the first sample to be detected, so as to detect a first detection item for the first sample to be detected.

[0075] The pressure source 21 of the sample analyzer is connected to the back pool of the detection element 11 , and controls the pressure source 21 of the sample analyzer to draw the first sample to be detected through the micropores 111 , so that the blood cells in the first sample to be detected pass through the micropores 111 one by one.

[0076] The constant current source 22 of the sample analyzer is electrically connected to the first electrode 12 and the second electrode 13. The constant current source 22 supplies power to the first electrode 12 and the second electrode 13 to obtain an electrical signal through the first electrode 12 and the second electrode 13 to count the first sample to be detected, thereby detecting the first detection item for the first sample to be detected.

[0077] S303: When the first test item of the first sample to be tested is completed, the sample to be tested is added to the test piece to be mixed with the remaining first sample to be tested in the test piece to obtain a second sample to be tested; or, the remaining first sample to be tested is discharged and the second sample to be tested is added to the test piece.

[0078] When the first test item of the first test sample is completed, the test piece 11 is added to the sample to be tested, and the sample to be tested is used to mix with the remaining first sample to be tested in the test piece 11 to obtain a second sample to be tested, wherein the dilution factor of the sample to be tested is less than or equal to the dilution factor of the first sample to be tested.

[0079] Alternatively, when the first test item of the first test sample is completed, the remaining first test sample is discharged, and the second test sample is added to the test piece 11. For example, the sample analyzer sucks out the remaining first test sample in the test piece 11.

[0080] S304: Control the sample analyzer to draw the second sample to be detected through the micropore 111, and obtain electrical signals through the first electrode 12 and the second electrode 13 to count the second sample to be detected, so as to detect a second detection item for the second sample to be detected.

[0081] The pressure source 21 of the sample analyzer is controlled to draw the second sample to be detected through the micropores 111, so that the blood cells in the second sample to be detected pass through the micropores 111 one by one. The first electrode 12 and the second electrode 13 are powered by the constant current source 22, so that the electrical signal is obtained through the first electrode 12 and the second electrode 13 to count the second sample to be detected, thereby realizing the detection of the second detection item on the second sample to be detected.

[0082] This embodiment controls the sample analyzer to detect the first detection item on the first sample to be detected through the detection element 11, and detects the second detection item on the second sample to be detected through the same detection element 11, so that the first detection item and the second detection item can be detected by the same detection element 11, reducing the cost of the single-channel detection component 10 and the cost of consumables. In addition, when the first detection item of the first sample to be detected is completed, the detection element 11 is added to the sample to be detected or the second sample to be detected, and there is no need to clean the detection element 11, which saves the cleaning system, further reduces the cost, and improves the detection efficiency.

[0083] According to some embodiments of the present application, the detection method of this embodiment further includes:

[0084] In response to completing the first test item of the first sample to be tested, and the liquid level of the first sample to be tested in the testing member 11 is higher than the preset liquid level H1, the sample analyzer is controlled to continue to draw the first sample to be tested through the micropore 111, so that the liquid level of the first sample to be tested is at or below the preset liquid level H1; wherein the preset liquid level H1 is at or below the plane where the central axis of the micropore 111 is located.

[0085] The central axis of the micropore 111 may be the center line of the micropore 111, and the preset liquid level H1 is located at or below the plane where the center line of the micropore 111 is located. Optionally, the plane where the central axis of the micropore 111 is located is a plane parallel to the liquid surface of the first sample to be detected in the detection element 11.

[0086] In response to completing the first test item of the first sample to be tested, and the liquid level of the first sample to be tested in the testing piece 11 is higher than the preset liquid level H1, the sample analyzer is controlled to continue to aspirate the first sample to be tested in the testing piece 11 through the micropore 111 until the liquid level of the first sample to be tested in the testing piece 11 is at or below the preset liquid level H1, so as to ensure that the liquid level of the first sample to be tested is at or below the preset liquid level H1, reduce the impact on the second sample to be tested, and improve the accuracy of the detection of the second sample to be tested.

[0087] According to some embodiments of the present application, the detection method of this embodiment further includes:

[0088] In response to completing the first test item of the first sample to be tested, and the liquid level of the first sample to be tested in the testing piece 11 is higher than the preset liquid level H1, the pipette 14 is controlled to aspirate the first sample to be tested in the testing piece 11 so that the liquid level of the first sample to be tested is lower than the preset liquid level H1; wherein the preset liquid level H1 is lower than the plane where the central axis of the micropore 111 is located, and is close to the bottom of the testing piece 11.

[0089] The pipette 14 is controlled to move into the detection member 11 to aspirate the first sample to be detected in the detection member 11 until the liquid level of the first sample to be detected in the detection member 11 is at or below the preset liquid level H1, so as to ensure that the liquid level of the first sample to be detected is at or below the preset liquid level H1, reduce the impact on the second sample to be detected, and improve the accuracy of detecting the second sample to be detected.

[0090] In addition, the preset liquid level H1 is as close to the bottom of the detection element 11 as possible, so as to further reduce the influence on the second sample to be detected and improve the accuracy of the detection of the second sample to be detected.

[0091] According to some embodiments of the present application, the difference between the initial liquid level of the first sample to be detected in the detection element 11 and the preset liquid level H1 ranges from 10 μL to 5000 μL.

[0092] According to some embodiments of this application, see Figure 4 As shown, Figure 4 is a flow chart of another embodiment of the detection method of the present application. After step S304, the detection method further includes the following steps:

[0093] S401 : in response to completing the second inspection item of the second sample to be inspected, controlling the light source 23 to generate light so as to irradiate the second sample to be inspected in the inspection element 11 .

[0094] The sample analyzer controls the light source 23 to generate light so that the light is irradiated to the second sample to be detected in the detection element 11 .

[0095] S402 : receiving the light passing through the second sample to be detected by the receiver 24 to detect the third detection item of the second sample to be detected.

[0096] The sample analyzer controls the receiver 24 to receive the light passing through the second sample to be detected, and the sample analyzer detects the second sample to be detected by photoelectric colorimetry, so that the sample analyzer detects the third detection item of the second sample to be detected.

[0097] The present application also provides a test kit, comprising a box body and the above-mentioned detection component, wherein the detection component is located on the box body. The detection component can be integrally formed with the box body; or the detection component and the box body are detachably connected, and the detection component is arranged on the box body.

[0098] The present application also provides a POCT blood cell analyzer, which includes a detection seat, a pressure source 21 and a constant current source 22. The detection seat is used to place the test kit of the above embodiment, the pressure source 21 is used to apply pressure to the detection piece 11 of the test kit so that the first sample to be detected or the second sample to be detected in the detection piece 11 passes through the micropore 111 of the detection piece 11; the constant current source 22 is used to power the first electrode 12 and the second electrode 13 of the single-channel detection component 10.

[0099] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A single-channel detection component, characterized in that: Applied to a sample analyzer, the single-channel detection component comprises: A detection member, used for accommodating a first sample to be detected, wherein the detection member is provided with micropores; A first electrode and a second electrode, wherein the first electrode and the second electrode are respectively located on two sides of the micropore; The detection component is installed in the sample analyzer, and the sample analyzer sucks the first sample to be detected through the micropore, and obtains electrical signals through the first electrode and the second electrode to count the first sample to be detected, so as to detect a first detection item on the first sample to be detected; When the first test item of the first sample to be tested is completed, the test piece is added to the sample to be tested, and is used to mix with the remaining first sample to be tested in the test piece to obtain a second sample to be tested, and the dilution factor of the sample to be tested is less than or equal to the dilution factor of the first sample to be tested; or, the remaining first sample to be tested is discharged, and the test piece is added to the second sample to be tested; The sample analyzer draws the second sample to be detected through the micropore, and obtains electrical signals through the first electrode and the second electrode to count the second sample to be detected, so as to detect a second detection item on the second sample to be detected.

2. The single-channel detection assembly according to claim 1, characterized in that: The first sample to be detected includes red blood cells or platelets, and the second sample to be detected includes white blood cells; When the first test item of the first sample to be tested is completed and the liquid level of the first sample to be tested in the testing member is higher than the preset liquid level, the sample analyzer continues to draw the first sample to be tested through the micropore; Alternatively, the single-channel detection component includes a pipette. When the first detection item of the first sample to be detected is completed and the liquid level of the first sample to be detected in the detection piece is higher than the preset liquid level, the pipette is used to aspirate the first sample to be detected in the detection piece so that the liquid level of the first sample to be detected is at or below the preset liquid level.

3. The single-channel detection assembly according to claim 1 or 2, characterized in that: The preset liquid level is located at or below the plane where the central axis of the micropore is located, and the difference between the initial liquid level of the first sample to be detected and the preset liquid level ranges from 10 μL to 5000 μL.

4. A detection method, characterized in that: Applied to the single-channel detection component according to any one of claims 1 to 3, the detection method comprises: Adding a first sample to be tested into the testing component, and installing the testing component on a sample analyzer; Controlling the sample analyzer to draw the first sample to be detected through the micropore, and obtaining electrical signals through the first electrode and the second electrode to count the first sample to be detected, so as to detect a first detection item on the first sample to be detected; When the first test item of the first sample to be tested is completed, the sample to be tested is added to the testing member to be mixed with the remaining first sample to be tested in the testing member to obtain a second sample to be tested, and the dilution factor of the sample to be tested is less than or equal to the dilution factor of the first sample to be tested; or, the remaining first sample to be tested is discharged, and the second sample to be tested is added to the testing member; The sample analyzer is controlled to draw the second sample to be detected through the micropore, and the electrical signal is obtained through the first electrode and the second electrode to count the second sample to be detected, so as to detect a second detection item on the second sample to be detected.

5. The detection method according to claim 4, characterized in that: The first sample to be detected includes red blood cells or platelets, the second sample to be detected includes white blood cells, and the detection method further includes: In response to completing the first test item of the first sample to be tested, and the liquid level of the first sample to be tested in the testing member is higher than a preset liquid level, controlling the sample analyzer to continue to draw the first sample to be tested through the micropores so that the liquid level of the first sample to be tested is at or below the preset liquid level; Wherein, the preset liquid level is located at or below the plane where the central axis of the microhole is located.

6. The detection method according to claim 4, characterized in that: The single-channel detection component includes a pipette, and the detection method further includes: In response to completing the first test item of the first sample to be tested, and the liquid level of the first sample to be tested in the testing member is higher than a preset liquid level, controlling the pipette to aspirate the first sample to be tested in the testing member so that the liquid level of the first sample to be tested is lower than the preset liquid level; Wherein, the preset liquid level is lower than the plane where the central axis of the microhole is located and is close to the bottom of the detection element.

7. The detection method according to claim 5 or 6, characterized in that: The difference between the initial liquid level of the first sample to be detected and the preset liquid level ranges from 10 μL to 5000 μL.

8. The detection method according to claim 4, characterized in that: The sample analyzer includes a light source and a receiver, and the detection method includes: In response to completing the second inspection item of the second sample to be inspected, controlling the light source to generate light so that the light irradiates the second sample to be inspected in the inspection member; The light passing through the second sample to be detected is received by the receiver to detect a third detection item of the second sample to be detected.

9. A kit, characterized in that: It comprises a box body and a single-channel detection component as described in any one of claims 1 to 3, wherein the single-channel detection component is located on the box body.

10. A POCT blood cell analyzer, characterized in that: It comprises a detection seat, a pressure source and a constant current source, wherein the detection seat is used to place the test kit as claimed in claim 9, and the pressure source is used to apply pressure to the detection element of the test kit so that the first sample to be detected or the second sample to be detected in the detection element passes through the micropores of the detection element; The constant current source is used to supply power to the first electrode and the second electrode of the detection component.

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