Composition for coating plant seeds

Abstract

Disclosed is a composition for coating a plant seed, which is capable of promoting the initial growth of a plant or promoting the growth under stress conditions. A composition for coating a plant seed according to one or more embodiments of the present invention contains glutathione, an amino acid, and an agriculturally acceptable carrier. The glutathione is preferably an oxidized glutathione, and the amino acid is preferably L-tryptophan. Another embodiment of the present invention relates to a coated seed comprising a seed of a plant and a composition comprising glutathione and an amino acid present on the surface of the seed.

Description

Technical Field

[0001] The present specification discloses a composition for coating plant seeds, coated seeds, a method for cultivating plants, a composition for promoting the initial growth of plants, and a composition for promoting the growth of plants under stress conditions. Background Art

[0002] As a method for applying a component useful for plant growth to a plant, a method of coating seeds with a composition containing a useful component is known. Compared with a method of applying a useful component to a leaf surface, soil or water, this method can efficiently supply the useful component to plant seeds with a small amount of the useful component. Therefore, seed treatment based on a composition containing a useful component is expected to be used as a method for supplying useful components to plants with a small environmental burden and low cost.

[0003] Patent Document 1 describes a seed treatment composition comprising aromatic amino acids such as tryptophan, phenylalanine, and tyrosine and gibberellins. In addition, Patent Document 1 describes that wheat seed treatment based on tryptophan promotes germination of wheat seeds under normal conditions and high salt concentration conditions. In addition, Patent Document 1 describes that wheat seed treatment based on tryptophan and gibberellins can synergistically promote the increase in fresh weight of seedlings under normal conditions and high salt concentration conditions.

[0004] Patent Document 2 describes that the rooting promoting effect of plants can be greatly enhanced by combining phenyllactic acid with tryptophan, which has a rooting promoting effect on plants. Patent Document 2 also describes that seeds are treated with a phenyllactic acid-tryptophan mixture.

[0005] On the other hand, glutathione is a peptide composed of three amino acids, L-cysteine, L-glutamic acid, and glycine. Glutathione exists in the body in the form of reduced glutathione (N-(N-γ-L-glutamyl-L-cysteinyl) glycine, hereinafter sometimes referred to as "GSH") and oxidized glutathione (hereinafter sometimes referred to as "GSSG"), wherein the reduced glutathione is a form of SH in which the thiol group of the L-cysteine ​​residue is reduced, and the oxidized glutathione is a form in which the thiol group of the L-cysteine ​​residue of two molecules of GSH is oxidized to form a disulfide bond between the two molecules of glutathione.

[0006] Patent Document 3 describes rice seeds treated with at least one coating material selected from a specific benzoxazole compound, iron and its oxide, calcium peroxide, molybdenum and a molybdenum compound, and at least one active ingredient selected from a plant growth regulator containing oxidized glutathione.

[0007] Patent document 4 describes a seed treatment composition comprising a carrier and an effective amount of glutathione or a salt thereof for promoting plant growth. In patent document 4, glutathione specifically refers to reduced glutathione (GSH). Patent document 4 describes that when one or more plant signaling molecules are further added to the seed treatment composition, the plant growth promoting effect based on the plant signaling molecules is synergistically improved. As specific examples of the plant signaling molecules, lipo-chitooligosaccharide compounds and chitooligosaccharide are described.

[0008] Prior art literature

[0009] Patent Literature

[0010] Patent Document 1: International Publication WO2018 / 215783

[0011] Patent Document 2: International Publication No. WO2009 / 104405

[0012] Patent Document 3: Japanese Patent Application Publication No. 2017-113033

[0013] Patent Document 4: International Publication No. WO2014 / 070757 Summary of the invention

[0014] Problems to be solved by the invention

[0015] In the cultivation of crop plants, it is important to promote the initial growth immediately after germination from seeds in order to increase the final yield. Therefore, a method for promoting the initial growth of plants is needed.

[0016] Additionally, there is a need for a method for improving plant growth under stress conditions such as salt stress.

[0017] Therefore, the present specification discloses a composition for coating plant seeds that can promote the initial growth of plants or promote the growth under stress conditions.

[0018] In addition, the present specification discloses a method for growing coated seeds and plants using the above-mentioned composition.

[0019] In addition, the present specification discloses a composition for promoting the initial growth of a plant and a composition for promoting the growth of a plant under stress conditions.

[0020] Solution to the problem

[0021] This specification discloses one or more of the following embodiments.

[0022] (1) A composition for coating plant seeds, comprising glutathione, amino acids, and an agriculturally acceptable carrier.

[0023] (2) The composition according to (1), wherein the glutathione is oxidized glutathione.

[0024] (3) The composition according to (1) or (2), wherein the amino acid is L-tryptophan.

[0025] (4) The composition according to any one of (1) to (3), comprising 0.012 parts by weight or more and 167 parts by weight or less of the amino acid per 100 parts by weight of the glutathione.

[0026] (5) The composition according to any one of (1) to (4), wherein the composition contains 3 g or more of the glutathione in an amount for coating 1 ton of plant seeds.

[0027] (6) The composition according to any one of (1) to (5), wherein the composition contains 0.009 g or more of the amino acid in an amount for coating 1 ton of plant seeds.

[0028] (7) The composition according to any one of (1) to (6), which is used for promoting initial growth of a plant.

[0029] (8) The composition according to any one of (1) to (7), which is used for promoting the growth of a plant under stress conditions.

[0030] (9) The composition according to any one of (1) to (8), which is used for increasing plant yield.

[0031] (10) A coated seed comprising:

[0032] Plant seeds, and

[0033] A composition comprising glutathione and amino acids present on the surface of the above seeds.

[0034] (11) The coated seed according to (10), wherein the glutathione is oxidized glutathione.

[0035] (12) The coated seed according to (10) or (11), wherein the amino acid is L-tryptophan.

[0036] (13) The coated seed according to any one of (10) to (12), wherein the composition contains 0.012 parts by weight or more and 167 parts by weight or less of the amino acid per 100 parts by weight of the glutathione.

[0037] (14) The coated seed according to any one of (10) to (13), wherein the coated seed contains 3 g or more of the glutathione per ton.

[0038] (15) The coated seed according to any one of (10) to (14), wherein the coated seed contains 0.009 g or more of the amino acid per ton.

[0039] (16) A method for cultivating a plant, the method comprising:

[0040] sowing the coated seeds according to any one of (10) to (15), and

[0041] The plants are grown from the sown coated seeds.

[0042] (17) The method according to (16), wherein the step of growing the plant includes promoting initial growth of the plant.

[0043] (18) The method according to (16) or (17), wherein the step of growing the plants includes increasing the density of the plants, wherein the density is a value obtained by dividing the fresh weight of the aboveground parts of the plants by the plant height of the aboveground parts of the plants.

[0044] (19) The method according to any one of (16) to (18), wherein the step of growing the plant includes increasing the yield of the plant.

[0045] (20) The method according to any one of (16) to (19), wherein the step of growing the plant includes growing the plant under stress conditions.

[0046] (21) A composition for promoting initial growth of a plant, comprising glutathione and 0.012 parts by weight or more and 167 parts by weight or less of an amino acid per 100 parts by weight of the glutathione.

[0047] (22) A composition for promoting growth of a plant under stress conditions, comprising glutathione and 0.012 parts by weight or more and 167 parts by weight or less of an amino acid per 100 parts by weight of the glutathione.

[0048] (23) A composition for increasing plant yield, comprising glutathione and 0.012 parts by weight or more and 167 parts by weight or less of an amino acid per 100 parts by weight of the glutathione.

[0049] This specification includes the disclosure of Japanese Patent Application No. 2022-036616 which is the basis of the priority of this application.

[0050] Effects of the Invention

[0051] The composition for coating plant seeds, the coated seeds using the composition, and the method for growing plants according to one or more embodiments of the present invention are effective for promoting the initial growth of plants and promoting the growth under stress conditions.

[0052] The composition for promoting the initial growth of a plant according to one or more embodiments of the present invention can promote the initial growth of a plant by being applied to the plant.

[0053] The composition for promoting the growth of plants under stress conditions according to one or more embodiments of the present invention can promote the growth of plants under stress conditions by being applied to plants. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] Figure 1 The graph shows the change rate (%) of the aboveground fresh weight of corn cultivated under normal conditions from seeds treated with different amounts of tryptophan (Trp) and oxidized glutathione (GSSG) in Example 1 relative to that of the control plot.

[0055] Figure 2 The graph shows the change rate (%) of the density of corn cultivated under normal conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 1 relative to that of the control plot.

[0056] Figure 3 The graph shows the change rate (%) of the aboveground fresh weight of corn grown under salt stress conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 2 relative to the control plot.

[0057] Figure 4 The figures show the plant height, leaf number, aboveground fresh weight, and density of corn grown from seeds treated with different amounts of tryptophan and oxidized glutathione under salt stress conditions in Example 2.

[0058] Figure 5 The graph shows the change rate (%) of the above-ground fresh weight of wheat grown under normal conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 3 relative to that of the control plot.

[0059] Figure 6 The graph shows the change rate (%) of the density of wheat cultivated under normal conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 3 relative to that of the control plot.

[0060] Figure 7The graph shows the change rate (%) of the aboveground fresh weight of wheat grown under salt stress conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 4 relative to that of the control plot.

[0061] Figure 8 The graph shows the change rate (%) of plant height, leaf number, aboveground fresh weight and density of wheat grown under salt stress conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 4 relative to the control plot.

[0062] Fig. 9 The graph shows the change rate (%) of the above-ground fresh weight of soybeans grown under normal conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 5 relative to that of the control plot.

[0063] Fig.10 The graph shows the change rate (%) of the density of soybeans grown under normal conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 5 relative to that of the control plot.

[0064] Fig.11 The graph shows the change rate (%) of the aboveground fresh weight of soybeans grown under salt stress conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 6 relative to that of the control plot.

[0065] Fig.12 The graph shows the change rate (%) of the above-ground fresh weight, density, and underground dry weight of soybeans grown under salt stress conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 6 relative to the control plot.

[0066] Fig.13 The graph shows the change rate (%) of the above-ground fresh weight of rice grown under normal conditions from seeds treated with different amounts of tryptophan and oxidized glutathione in Example 7 relative to that of the control plot.

[0067] Fig.14 The graph shows the change rate (%) of the above-ground fresh weight of corn grown under normal conditions from seeds treated with different amounts of tryptophan and reduced glutathione in Comparative Example 1 relative to that of the control plot.

[0068] Fig.15 The graph shows the change rate (%) of the aboveground fresh weight of corn grown under salt stress conditions from seeds treated with different amounts of tryptophan and reduced glutathione in Comparative Example 2 relative to that of the control plot.

[0069] Fig.16The ear yield of corn cultivated in the field from corn seeds coated with 0.25 g tryptophan per ton of seeds, corn seeds coated with 75 g oxidized glutathione per ton of seeds, and seeds coated with 0.25 g tryptophan and 75 g oxidized glutathione per ton of seeds in Example 8 is shown. DETAILED DESCRIPTION

[0070] Hereinafter, one or more embodiments of the present invention will be described.

[0071] <Glutathione>

[0072] In the present specification, "glutathione" may be reduced glutathione (GSH, N-(N-γ-L-glutamyl-L-cysteinyl)glycine), or oxidized glutathione (GSSG) in which two GSH molecules are bonded via a disulfide bond, preferably GSSG.

[0073] Glutathione (GSSG or GSH) may include various forms of glutathione, such as a free form that is not bound to other substances and is not ionized, a salt formed of glutathione and an acid or a base, a hydrate thereof, and a mixture thereof.

[0074] When GSSG is used as glutathione, it can be used in the form of a mixture of GSSG and GSH, and the content of GSSG is preferably relatively larger than the content of GSH. With respect to the total mass of GSSG and GSH (converted to the mass of all free bodies), the total mass of GSSG (converted to the mass of free bodies) is more preferably 70% by mass or more, more preferably 80% by mass or more, more preferably 90% by mass or more, further preferably 95% by mass or more, further preferably 98% by mass or more, and most preferably 100% by mass.

[0075] The salt of GSSG is not particularly limited as long as it is one or more salts allowed as fertilizers such as ammonium salts, calcium salts, magnesium salts, sodium salts, and lithium salts, but is preferably one or more salts selected from ammonium salts, calcium salts, and magnesium salts. Examples of the GSSG salt include monoammonium salt of GSSG, 0.5 calcium salt or monocalcium salt of GSSG, and 0.5 magnesium salt or monomagnesium salt of GSSG.

[0076] <Amino Acid>

[0077] In this specification, "amino acid" is any amino acid that is agriculturally acceptable, preferably tryptophan. When the amino acid is an optically active amino acid, it may be an L-form, a D-form, or a mixture of an L-form and a D-form, preferably an L-form. The amino acid may be only one amino acid or a mixture of two or more amino acids. The most preferred amino acid is L-tryptophan.

[0078] The amino acid may include various forms of amino acids, such as free forms that are not bound to other substances and are not ionized, salts formed from amino acids and acids or bases, hydrates thereof, and mixtures thereof.

[0079] <Agriculturally acceptable carriers>

[0080] In this specification, "agriculturally acceptable carrier" can be any carrier that can hold glutathione and amino acids, and can be a liquid carrier or a solid carrier. As a solid carrier, a hydratable solid can be mentioned. The solid carrier can be in the form of powder or granules.

[0081] Preferred "agriculturally acceptable carriers" are liquid carriers such as water and organic solvents. Here, water as a carrier is not limited to pure water, and may be an aqueous solution, an aqueous suspension, an aqueous gel or an aqueous slurry, and may have viscosity. Similarly, the organic solvent is not limited to a pure organic solvent, and may be a solution, a suspension, a gel or a slurry based on an organic solvent, and may have viscosity. Examples of the organic solvent include methyl ether, ethyl ether, propyl ether and butyl ether.

[0082] The carrier that is agriculturally permissible is preferably a liquid carrier of an aqueous solution formed by dissolving a hydratable substance in water, or a solid carrier comprising a hydratable substance that can be dissolved in water. As the hydratable substance, examples include: polyvinyl pyrrolidone, random and block copolymers of alkylene oxide, vinyl acetate / vinyl pyrrolidone copolymers, alkylated vinyl pyrrolidone copolymers, polyalkylene glycols comprising polypropylene glycol and polyethylene glycol, polyvinyl acetate, polyvinyl alcohol, gelatin, agar, gum arabic, karaya gum, tragacanth gum, guar gum, locust bean gum, xanthan gum, ghatti gum, carrageenan, alginate, casein, dextran, pectin, chitin, 2-hydroxyethyl starch, 2-aminoethyl starch, 2-hydroxyethyl cellulose, methyl cellulose, carboxymethyl cellulose salts, cellulose sulfate, polyacrylic acid amide, alkali metal salts of maleic anhydride copolymers, alkali metal salts of poly (meth) acrylates, etc.

[0083] Plant seed coating composition can further comprise one or more of the following further additives as required. As additives, wetting agent, colorant, defoamer, UV protectant, antifreeze, preservative, biological control agent or biocide, surfactant, emulsifier, extender, capture agent, plasticizer, phospholipid, flow agent, fusion aid, wax, and / or filler (for example, clay, talcum, glass fiber, cellulose, micronized wood, etc.) can be listed, but it is not limited to this.

[0084] <Plants>

[0085] In one or more embodiments of the present invention, "plant" is not particularly limited, and is preferably a crop plant. Examples of crop plants include corn (corn, maize), wheat, barley, rye, oats, rice, soybeans, rapeseed (Brassica napus), cotton, sunflowers, sugar beets, potatoes, tobacco, broccoli, lettuce, cabbage, cauliflower, coconut, tomato, cucumber, eggplant, honeydew melon, pumpkin, okra, pepper, watermelon, carrot, radish, onion, onion, flowers, lawn grass, forage grass, etc. In one or more embodiments of the present invention, "seed" can be seeds of these plants.

[0086] <Composition for coating plant seeds>

[0087] A first embodiment of the present invention relates to a composition for coating plant seeds, comprising glutathione, amino acids, and an agriculturally acceptable carrier.

[0088] By treating the seeds of the plant with the composition for coating plant seeds of the present embodiment, the growth of the seeds toward the plant, especially the initial growth after germination, can be promoted. Here, "initial growth" generally refers to the middle of the vegetative growth period from germination to the beginning of a rapid increase in dry matter production. For example, it refers to growth within 90 days, within 60 days, within 30 days, within 25 days, within 21 days, or within 14 days after germination.

[0089] In addition, by treating plant seeds with the composition for coating plant seeds according to the present embodiment, growth under stress conditions such as salt stress can be promoted.

[0090] The value obtained by dividing the fresh weight of the aboveground part of the plant by the plant height is defined as "density". The composition for coating plant seeds of this embodiment is particularly capable of increasing the "density" of plants. According to the composition for coating plant seeds of this embodiment, these effects can be used to increase the yield of plants. Here, the increase in plant yield refers to the increase in the weight of the plant parts harvested as crops, and is particularly preferably the increase in the weight of fruits, seeds, leaves or stems harvested as crops.

[0091] That is, the composition for coating plant seeds of this embodiment is a composition for promoting the initial growth of plants, a composition for promoting the growth of plants under stress conditions, a composition for increasing the yield of plants, or a composition for increasing the density of plants (as defined above).

[0092] According to the different forms of the above-mentioned agriculturally allowable carrier, the seed plant coating composition of the present embodiment can be liquid or solid accordingly. When the seed plant coating composition of the present embodiment is a liquid composition, the seed of plant can be immersed or sprayed with the above-mentioned liquid composition and coated, and dried as required after coating. When the seed plant coating composition of the present embodiment is a solid composition such as powder, the above-mentioned solid composition after drying can be contacted with the seed of plant and coated, and it can also be contacted with the seed of plant and coated after adding water in the above-mentioned solid composition and making liquid or pasty state, and dried as required after coating.

[0093] In the composition for coating seeds and plants of the present embodiment, the glutathione is preferably oxidized glutathione and the amino acid is L-tryptophan. By coating plant seeds with a combination of oxidized glutathione and L-tryptophan, the growth of the plant can be particularly significantly promoted.

[0094] The seed plant coating composition of the present embodiment contains 0.012 weight parts or more of amino acids relative to 100 weight parts of glutathione, preferably contains 0.015 weight parts or more, and more preferably contains 0.030 weight parts or more, and the upper limit contains 167 weight parts or less, preferably contains 84 weight parts or less, and particularly preferably contains 67 weight parts or less relative to 100 weight parts of glutathione. When the weight ratio of glutathione to amino acids is within this range, the effect of synergistically promoting plant growth is particularly significant.

[0095] The concentrations of glutathione and amino acids in the composition for coating seeds and plants of the present embodiment are not particularly limited. The above-mentioned composition for coating seeds and plants in the amount used for coating 1 ton of plant seeds preferably contains glutathione (especially oxidized glutathione) 3g or more, more preferably 6g or more, and particularly preferably 15g or more, and the upper limit is not particularly limited, preferably 500g or less, more preferably 300g or less. In addition, the above-mentioned composition for coating seeds and plants in the amount used for coating 1 ton of plant seeds preferably contains amino acids (especially L-tryptophan) 0.009g or more, more preferably 0.040g or more, and particularly preferably 0.10g or more, and the upper limit is not particularly limited, preferably 50g or less, more preferably 30g or less, and particularly preferably 10g or less.

[0096] <Coated seeds>

[0097] A second embodiment of the present invention relates to a coated seed, comprising:

[0098] Plant seeds, and

[0099] A composition comprising glutathione and amino acids present on the surface of the above seeds.

[0100] The coated seeds of the present embodiment can be prepared by coating plant seeds with the composition for coating plant seeds of the first embodiment of the present invention.

[0101] Plants grown from the coated seeds of the present embodiment have high initial growth properties and high growth properties under stress conditions.

[0102] In the coated seeds of the present embodiment, the composition containing glutathione and amino acids present on the surface of the seeds is typically the composition for coating plant seeds of the first embodiment of the present invention itself, or a composition obtained by drying the composition for coating plant seeds. It should be noted that in the coated seeds of the present embodiment, the composition containing glutathione and amino acids is not limited to being present only on the surface of the seeds, and a part of the composition can penetrate into the inside of the seeds.

[0103] In the coated seeds of this embodiment, the glutathione is preferably oxidized glutathione and the amino acid is L-tryptophan. By coating plant seeds with a combination of oxidized glutathione and L-tryptophan, plant growth can be particularly significantly promoted.

[0104] The above-mentioned composition in the coated seeds of the present embodiment particularly preferably contains 0.012 weight parts or more and 167 weight parts or less of amino acids relative to 100 weight parts of glutathione. The lower limit is not particularly limited as long as it is 0.012 weight parts or more, preferably 0.015 weight parts or more, and more preferably 0.030 weight parts or more. In addition, the upper limit is not particularly limited as long as it is 167 weight parts or less, preferably 84 weight parts or less, and more preferably 67 weight parts or less. When the weight ratio of glutathione to amino acids is within this range, the effect of synergistically promoting plant growth is particularly significant.

[0105] The content of glutathione and amino acids in the coated seeds of the present embodiment is not particularly limited. On average, the coated seeds of the present embodiment preferably contain 3 g or more of glutathione (especially oxidized glutathione), more preferably 6 g or more, and particularly preferably 15 g or more, and the upper limit is not particularly limited, preferably 500 g or less, and more preferably 300 g or less. In addition, the coated seeds of the present embodiment preferably contain 0.009 g or more of amino acids (especially L-tryptophan), more preferably 0.040 g or more, and particularly preferably 0.10 g or more, and the upper limit is not particularly limited, preferably 50 g or less, more preferably 30 g or less, and particularly preferably 10 g or less.

[0106] <How to grow plants>

[0107] A third embodiment of the present invention relates to a method for cultivating a plant, the method comprising:

[0108] sowing the coated seeds according to the second embodiment of the present invention, and

[0109] The plants are grown from the sown coated seeds.

[0110] According to the method of the third embodiment of the present invention, the growth of plants, especially the initial growth, can be promoted, and plants can be cultivated with high yield. In addition, plants can be cultivated efficiently even under stress conditions such as salt stress.

[0111] In the method of the present embodiment, the step of sowing the coated seeds and the step of growing the plant from the sown coated seeds can be performed under conditions appropriately selected according to the plant.

[0112] In the method of this embodiment, in the step of growing the plant from the coated seeds after sowing, growth, especially initial growth, is promoted. Here, the promotion of growth is preferably an increase in the fresh weight and / or density (defined as above) of the aboveground part of the plant.

[0113] The degree of increase in the aboveground fresh weight of the method according to the present embodiment is not particularly limited, and the increase in the aboveground fresh weight is preferably 1% or more, more preferably 3% or more, further preferably 5% or more, and particularly preferably 10% or more, compared to the case where the plant is cultivated from seeds that are not coated with the above-mentioned plant seed coating composition. From the viewpoint of market value, the upper limit of the increase in the above-ground fresh weight is not particularly limited, but is preferably 100% or less, more preferably 80% or less, and particularly preferably 60% or less.

[0114] The degree of increase in density based on the method of this embodiment is not particularly limited, and the rate of increase in density is preferably 1% or more, more preferably 3% or more, further preferably 5% or more, and particularly preferably 10% or more, compared to the case where plants are grown from seeds that are not coated with the above-mentioned plant seed coating composition. From the viewpoint of market value, the upper limit of the rate of increase in density is not particularly limited, but is preferably 100% or less, more preferably 80% or less, and particularly preferably 60% or less.

[0115] In the method of this embodiment, the step of growing the plant from the coated seeds after sowing includes, for example, growing the plant under stress conditions. Here, as stress conditions, salt stress conditions, high temperature stress conditions, low temperature stress conditions, dryness stress conditions, etc. can be exemplified.

[0116] <Other Compositions>

[0117] A fourth aspect of the present invention relates to a composition for promoting initial growth of a plant, comprising glutathione and 0.012 parts by weight or more and 167 parts by weight or less of an amino acid per 100 parts by weight of the glutathione.

[0118] A fifth embodiment of the present invention relates to a composition for promoting plant growth under stress conditions, comprising glutathione and 0.012 parts by weight or more and 167 parts by weight or less of amino acids per 100 parts by weight of the glutathione.

[0119] A sixth embodiment of the present invention relates to a composition for increasing plant yield, comprising glutathione and 0.012 parts by weight or more and 167 parts by weight or less of amino acids per 100 parts by weight of the glutathione.

[0120] The compositions of the fourth, fifth and sixth embodiments of the present invention are not limited to seed coating compositions, and may be compositions for application to plants via appropriate routes, such as compositions for application to leaves or the like, compositions for application to soil or water.

[0121] The composition according to the fourth embodiment of the present invention can significantly promote the initial growth of plants particularly due to the synergistic effect of glutathione and amino acids.

[0122] The composition of the fifth embodiment of the present invention can significantly promote the initial growth of plants under stress conditions through the synergistic effect of glutathione and amino acids. Here, as stress conditions, salt stress conditions, high temperature stress conditions, low temperature stress conditions, dry stress conditions, etc. can be exemplified.

[0123] The composition according to the sixth embodiment of the present invention can significantly increase the yield of plants due to the synergistic effect of glutathione and amino acids.

[0124] In the fourth, fifth and sixth aspects of the present invention, it is more preferred that glutathione is oxidized glutathione and the amino acid is L-tryptophan.

[0125] Example

[0126] The present invention will be specifically described by way of the following examples, but the present invention is not limited to the examples.

[0127] In the following description and the drawings to be referred to, "tryptophan" or "Trp" means L-tryptophan, "GSSG" means oxidized glutathione, and "GSH" means reduced glutathione.

[0128] <Example 1>

[0129] Corn cultivation from coated seeds under normal conditions

[0130] Experimental methods

[0131] Using a coating apparatus (manufactured by SATEC), 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and a coloring pigment in water was coated on 100 g of dent corn seeds (variety: Snow-dent Otoha). The coating treatment was performed individually or in combination with 0 g, 0.045 g, 0.1225 g, 0.45 g, 5 g, or 50 g of tryptophan per ton of seeds and 0 g, 3 g, 15 g, 150 g, or 300 g of oxidized glutathione per ton of seeds.

[0132] The soil was filled into 12 cm plastic pots, and 4 dent corn seeds were sown per pot after coating. After germination, one plant with different growth levels was thinned out to 3 plants per pot. The plants were cultivated in a glass greenhouse and investigated on the 21st day after sowing. During the investigation, plant height and aboveground fresh weight were measured. The density (aboveground fresh weight / plant height) was calculated. The test was repeated 6 times to obtain the average value.

[0133] result

[0134] Fresh weight of aboveground parts

[0135] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and oxidized glutathione are shown as the change rate (%) relative to the aboveground fresh weight of the test plot (control plot) treated with a coating solution containing no tryptophan and oxidized glutathione. Figure 1 .

[0136] according to Figure 1, when only tryptophan was applied to seeds, the aboveground fresh weight after 3 weeks of sowing was -6% to 4% relative to no treatment, and no initial growth promoting effect could be confirmed in the case of tryptophan alone. On the other hand, when only GSSG was applied to seeds, the aboveground fresh weight increased by 4% to 12%, and a certain initial growth promoting effect could be confirmed in the case of GSSG alone. In addition, by combining GSSG with tryptophan, the aboveground fresh weight increased by 9% to 29%, and the synergistic effect of GSSG and tryptophan showed an unexpected initial growth promoting effect. For example, when 0.1225g of tryptophan and 150g of GSSG per 1 ton of seeds were treated with seeds alone or in combination, the aboveground fresh weight increased by 3% in the case of tryptophan alone and 11% in the case of GSSG alone relative to no treatment. In contrast, by combining GSSG with tryptophan, it increased by 27%, showing a significant synergistic effect. It was also found that an initial growth promoting effect was observed by combining 3 g or more of GSSG and tryptophan per ton of seeds, and that a higher synergistic effect was exhibited when 15 g or more of GSSG was contained.

[0137] density

[0138] The density (aerial fresh weight / plant height) calculated for each test plot was expressed as a change rate (%) relative to the density of the control plot. Figure 2 .

[0139] Increased fresh weight of the aboveground part can also be considered as leggy growth, that is, the elongation growth of the plant is dominant without the growth of the body. If leggy growth occurs, the plant will be sickly / weak and less resistant to pests than a normally grown plant, and will also be easily affected by environmental changes such as heat and cold, so it is not preferred. Therefore, in order to evaluate the growth of short and strong quality rather than leggy growth, the density was used as an indicator for evaluation. Figure 2 It was confirmed that the density was synergistically increased in the case of the combination of GSSG and tryptophan as compared with the case of each of the two alone, and that an increase in yield due to promotion of initial growth can be expected.

[0140] <Example 2>

[0141] Maize cultivation from coated seeds under salt stress conditions

[0142] Experimental methods

[0143] Using a coating apparatus (manufactured by SATEC), 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and a coloring pigment in water was coated on 100 g of dent corn seeds (variety: Snow-dent Otoha). The coating treatment was performed individually or in combination with 0 g, 0.009 g, 0.045 g, 0.1225 g, 0.225 g, 0.45 g, or 5 g of tryptophan per ton of seeds and 0 g, 15 g, or 75 g of oxidized glutathione per ton of seeds.

[0144] The soil mixed with sodium chloride at 6.0 g / L was filled in a 7.5 cm pot, and dent corn seeds were sown 1 grain / pot after coating. The plants were cultivated in a glass greenhouse and investigated on the 21st day after sowing. During the investigation, plant height, aboveground fresh weight, and leaf number were measured, and density (aboveground fresh weight / plant height) was calculated. The test was repeated 9 times to obtain the average value.

[0145] result

[0146] Fresh weight of aboveground parts

[0147] The aboveground fresh weights of the test plots treated with different amounts of tryptophan and oxidized glutathione are shown as the change rate (%) relative to the aboveground fresh weight of the test plot (control plot) treated with the coating solution containing no tryptophan and oxidized glutathione. Figure 3 .

[0148] according to Figure 3 Under salt stress conditions, when only tryptophan was applied to seeds, the aboveground fresh weight 3 weeks after sowing was -13% to 3% relative to no treatment, and no initial growth promoting effect was confirmed in the case of tryptophan alone. In addition, when only GSSG was applied to seeds, it was 2% to 6% relative to no treatment, and no significant initial growth promoting effect was confirmed. On the other hand, by combining GSSG with tryptophan, the aboveground fresh weight increased by 3% to 29%, showing an unexpected initial growth promoting effect through the synergistic effect of GSSG and tryptophan. In particular, when GSSG and tryptophan were applied to seeds in a ratio of 0.012 parts by weight or more of tryptophan to 100 parts by weight of GSSG, a significant effect was confirmed.

[0149] Plant height, number of leaves, aboveground fresh weight, density

[0150] The change rate (%) of plant height, leaf number, aboveground fresh weight, and density (aboveground fresh weight / plant height) measured or calculated for each test plot relative to the control plot was calculated. The plant height, leaf number, aboveground fresh weight, and density of the control plot, 0.045 g tryptophan / 1 ton of seeds, 15 g oxidized glutathione / 1 ton of seeds, and 0.045 g tryptophan + 15 g oxidized glutathione / 1 ton of seeds are shown in Figure 4 .

[0151] according to Figure 4 When 0.045 g of tryptophan and 15 g of GSSG were combined per ton of seeds for seed treatment, plant height, leaf age, aboveground fresh weight, and density all increased synergistically when GSSG and tryptophan were combined compared to single treatments, and a stable growth-promoting effect brought about by seed treatment could be confirmed under stress conditions.

[0152] <Example 3>

[0153] Wheat cultivation from coated seeds under normal conditions

[0154] Experimental methods

[0155] A coating treatment was performed by coating 100 g of wheat seeds (Quintus) with 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and a coloring pigment in water using a coating device (manufactured by SATEC). The coating treatment was performed individually or in combination with 0 g, 0.01 g, 0.25 g, 5 g, or 10 g of tryptophan and 0 g, 6 g, or 300 g of oxidized glutathione per ton of seeds.

[0156] The soil was filled into a 96-hole tray, and one wheat seed per hole was sown after the coating treatment. The survey was conducted 2 weeks after sowing. During the survey, the plant height and aboveground fresh weight were measured, and the density (aboveground fresh weight / plant height) was calculated. The test was carried out with 6 individuals per test area × 4 replicates.

[0157] result

[0158] Fresh weight of aboveground parts

[0159] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and oxidized glutathione are shown as the change rate (%) relative to the aboveground fresh weight of the test plot (control plot) treated with a coating solution containing no tryptophan and oxidized glutathione. Figure 5 .

[0160] according to Figure 5In the case where only tryptophan was applied to the seeds, the aboveground fresh weight 2 weeks after sowing was -8% to 2% relative to that of no treatment, and no initial growth promoting effect was confirmed in the case of tryptophan alone. In addition, in the case where only GSSG was applied to the seeds, it was -1% to 0%, and no initial growth promoting effect was confirmed in the case of GSSG alone. On the other hand, by combining GSSG with tryptophan, the aboveground fresh weight increased by 1% to 8%, and an unexpected initial growth promoting effect was shown through the synergistic effect of GSSG and tryptophan. In particular, when GSSG and tryptophan were applied to the seeds in a ratio of 0.012 parts by weight or more of tryptophan to 100 parts by weight of GSSG, a significant effect was confirmed.

[0161] density

[0162] The density (aerial fresh weight / plant height) calculated for each test plot is shown as a change rate (%) relative to the density of the control plot. Figure 6 .

[0163] according to Figure 6 In the case of the combination of GSSG and tryptophan, the density also increased synergistically, so it was confirmed that the plants grew short and strong with good quality, and an increase in yield due to the promotion of initial growth could be expected.

[0164] <Example 4>

[0165] Wheat cultivation from coated seeds under salt stress conditions

[0166] Experimental methods

[0167] A coating treatment was performed by coating 100 g of wheat seeds (variety: Yumechikara) with 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and a coloring pigment in water using a coating device (manufactured by SATEC). The coating treatment was performed individually or in combination in a manner that tryptophan was 0 g, 0.225 g, 2.25 g, 5 g, 10 g, or 50 g per ton of seeds, and oxidized glutathione was 0 g, 15 g, or 300 g.

[0168] The soil mixed with sodium chloride at 4.0 g / L was filled in a 7.5 cm pot, and wheat seeds after coating were sown 1 grain / pot. The plants were cultivated in a glass greenhouse and investigated on the 16th day after sowing. During the investigation, plant height and aboveground fresh weight were measured, and density (aerial fresh weight / plant height) was calculated. The test was repeated 9 times to obtain an average value.

[0169] result

[0170] Fresh weight of aboveground parts

[0171] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and oxidized glutathione are shown as the change rate (%) relative to the aboveground fresh weight of the test plot (control plot) treated with a coating solution containing no tryptophan and oxidized glutathione. Figure 7 .

[0172] according to Figure 7 Under salt stress conditions, when only tryptophan was applied to seeds, the aboveground fresh weight 2 weeks after sowing was -17% to 3% relative to no treatment, and no initial growth promoting effect was confirmed in the case of tryptophan alone. In addition, when only GSSG was applied to seeds, it was -6% to 6% relative to no treatment, and no significant initial growth promoting effect was confirmed. On the other hand, by combining GSSG with tryptophan, the aboveground fresh weight increased by 5% to 16%, showing an unexpected initial growth promoting effect through the synergistic effect of GSSG and tryptophan.

[0173] Plant height, number of leaves, aboveground fresh weight, density

[0174] The change rate (%) of plant height, leaf number, aboveground fresh weight, and density (aboveground fresh weight / plant height) measured or calculated for each test plot relative to the control plot was calculated. The plant height, leaf number, aboveground fresh weight, and density of the control plot, 0.225 g tryptophan / 1 ton of seeds, 15 g oxidized glutathione / 1 ton of seeds, and 0.225 g tryptophan + 15 g oxidized glutathione / 1 ton of seeds are shown in Figure 8 .

[0175] according to Figure 8 When 0.225 g of tryptophan and 15 g of GSSG were combined per ton of seeds for seed treatment, plant height, leaf age, aboveground fresh weight, and density increased synergistically when GSSG and tryptophan were combined compared to single treatments, and a stable growth-promoting effect brought about by seed treatment could be confirmed under stress conditions.

[0176] <Example 5>

[0177] Soybean cultivation from coated seeds under normal conditions

[0178] Experimental methods

[0179] A coating treatment was performed by coating 100 g of soybean seeds (variety: Fukuyutaka or Lenka) with 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and a coloring pigment in water using a coating device (manufactured by SATEC). The coating treatment was performed individually or in combination in such a manner that tryptophan was 0 g, 0.05 g, 0.25 g, 0.5 g, or 5 g per ton of seeds, and oxidized glutathione was 0 g, 15 g, 30 g, or 300 g.

[0180] The soil was filled in a 96-hole tray, and one soybean seed per hole was sown after the coating treatment. The survey was conducted 3 weeks after sowing. During the survey, the plant height and aboveground fresh weight were measured, and the density (aboveground fresh weight / plant height) was calculated. The test was carried out with 6 individuals per test area × 4 replicates.

[0181] result

[0182] Fresh weight of aboveground parts

[0183] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and oxidized glutathione are shown as the change rate (%) of the aboveground fresh weight of the control plots treated with the coating solution containing no tryptophan and oxidized glutathione. Fig. 9 .

[0184] according to Fig. 9 Compared with tryptophan alone, the combination of GSSG and tryptophan showed a tendency of increasing the fresh weight of the aboveground parts. This shows that the synergistic effect of GSSG and tryptophan showed an unexpected initial growth promotion effect.

[0185] density

[0186] A portion of the density (aerial fresh weight / plant height) calculated for each test plot is shown as a change rate (%) relative to the density of the control plot. Fig.10 .

[0187] according to Fig.10 In the case of the combination of GSSG and tryptophan, the density also increased synergistically, so it was confirmed that the plants grew short and strong with good quality, and an increase in yield due to the promotion of initial growth could be expected.

[0188] <Example 6>

[0189] Soybean cultivation from coated seeds under salt stress conditions

[0190] Experimental methods

[0191] A coating treatment was performed by coating 100 g of soybean seeds (variety: Fukuyutaka) with 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and a coloring pigment in water using a coating device (manufactured by SATEC). The coating treatment was performed individually or in combination in such a manner that the tryptophan content was 0 g, 0.045 g, 0.225 g, 0.45 g, or 5 g per ton of seeds, and the oxidized glutathione content was 0 g, 15 g, or 75 g.

[0192] 7.5 cm pots were filled with soil mixed with sodium chloride at 1.0 g / L, and 1 soybean seed / pot was sown after the coating treatment. The plants were cultivated in a glass greenhouse and investigated on the 21st day after sowing. In the investigation, the aboveground fresh weight, density (aboveground fresh weight / plant height), and underground dry weight were measured. The test was repeated 9 times and the average value was calculated.

[0193] result

[0194] Fresh weight of aboveground parts

[0195] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and oxidized glutathione and the aboveground fresh weights of the test plots treated with the coating solution containing no tryptophan and oxidized glutathione (control plot) are shown as the change rate (%) relative to the aboveground fresh weight of the control plot. Fig.11 .

[0196] according to Fig.11 Under salt stress conditions, when only tryptophan was applied to seeds, the aboveground fresh weight 3 weeks after sowing was -2.5% to 1% relative to no treatment, and no initial growth promoting effect was confirmed in the case of tryptophan alone. In addition, when only GSSG was applied to seeds, it was -3.5% to -1% relative to no treatment, and no initial growth promoting effect was confirmed. On the other hand, by combining GSSG with tryptophan, the aboveground fresh weight increased by 1% to 10%, showing an unexpected initial growth promoting effect through the synergistic effect of GSSG and tryptophan.

[0197] Fresh weight of aboveground parts, density, dry weight of underground parts

[0198] The change rate (%) of the aboveground fresh weight, density (aboveground fresh weight / plant height) and underground dry weight measured or calculated for each test plot relative to the control plot was obtained. The aboveground fresh weight, density and underground dry weight of the control plot, tryptophan 0.225g / 1 ton of seeds, oxidized glutathione 75g / 1 ton of seeds, and tryptophan 0.225g + oxidized glutathione 75g / 1 ton of seeds are shown in Fig.12 .

[0199] according to Fig.12 When seeds were treated with a combination of 0.225 g of tryptophan and 75 g of GSSG per ton of seeds, the fresh weight of the aboveground part, density, and dry weight of the underground part all increased synergistically when GSSG and tryptophan were combined compared to single treatments, and a stable growth-promoting effect brought about by seed treatment could be confirmed under stress conditions.

[0200] <Example 7>

[0201] Rice cultivation from coated seeds under normal conditions

[0202] Experimental methods

[0203] A coating treatment was performed by coating 100 g of rice seeds (variety: Emimaru) with 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and a coloring pigment in water using a coating device (manufactured by SATEC). The coating treatment was performed individually or in combination in such a manner that the tryptophan content was 0 g, 2.25 g, or 5 g per ton of seeds, and the oxidized glutathione content was 0 g or 75 g.

[0204] 12 cm plastic pots were filled with soil, and 5 coated rice seeds were sown per pot. The rice was cultivated in a glass greenhouse, and the fresh weight of the aboveground part was investigated on the 21st day after sowing. The test was repeated 6 times, and the average value was calculated.

[0205] result

[0206] Fresh weight of aboveground parts

[0207] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and oxidized glutathione and the aboveground fresh weights of the test plots treated with the coating solution containing no tryptophan and oxidized glutathione (control plot) were expressed as the rate of change (%) relative to the aboveground fresh weight of the control plot. Fig.13 .

[0208] according to Fig.13 In the case of seed coating with tryptophan alone, the aboveground fresh weight three weeks after sowing was 23% to 26% relative to that without treatment, and in the case of seed coating with GSSG alone, it was 19% relative to that without treatment, showing a certain initial growth promotion effect. In addition, by combining GSSG with tryptophan, the aboveground fresh weight increased by 46% to 47%, indicating that the synergistic effect of GSSG and tryptophan showed an initial growth promotion effect greater than that of the single case.

[0209] <Comparative Example 1>

[0210] Cultivation of corn from coated seeds under normal conditions

[0211] Experimental methods

[0212] A coating treatment was performed using a coating apparatus (manufactured by SATEC) by coating 100 g of dent corn seeds (variety: Snow-dent Otoha) with 800 μL of a coating solution containing 0.225 g of tryptophan / 1 ton of seeds (MT-seed), 0, 1, 5, 15, and 75 g of reduced glutathione / MT-seed in water, polyvinyl alcohol, and a coloring pigment.

[0213] The soil was filled into 12 cm plastic pots, and 4 dent corn seeds were sown per pot after coating. After germination, one plant with different growth levels was thinned out to 3 plants per pot. The plants were cultivated in a glass greenhouse and investigated on the 21st day after sowing. During the investigation, the fresh weight of the aboveground parts was measured. The experiment was repeated 6 times and the average value was calculated.

[0214] result

[0215] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and reduced glutathione were expressed as a percentage change (%) relative to the aboveground fresh weight of the test plot (control plot) treated with a coating solution containing no tryptophan and reduced glutathione. Fig.14 .

[0216] according to Fig.14 In the case of seed coating with GSH (reduced glutathione) alone, the change rate of the aboveground fresh weight 3 weeks after sowing was 4% relative to that without treatment, and in the case of seed coating with GSH alone, it was 9% to 10% relative to that without treatment. In the case of combining GSH with tryptophan, it was 10% to 12%, and no synergistic effect was observed due to the combination of GSH and tryptophan.

[0217] <Comparative Example 2>

[0218] Maize cultivation from coated seeds under salt stress conditions

[0219] Experimental methods

[0220] A coating treatment was performed using a coating apparatus (manufactured by SATEC) by coating 100 g of dent corn seeds (variety: Snow-dent Otoha) with 800 μL of a coating solution containing 0.225 g / MT-seed of tryptophan, 0, 1, 5, 15, and 75 g / MT-seed of reduced glutathione, polyvinyl alcohol, and a coloring pigment in water.

[0221] 7.5 cm pots were filled with soil mixed with sodium chloride at 6.0 g / L, and dent corn seeds were sown one grain / pot after the coating treatment. The plants were cultivated in a glass greenhouse and investigated on the 21st day after sowing. During the investigation, the fresh weight of the aboveground parts was measured. The test was repeated 9 times and the average value was calculated.

[0222] result

[0223] The aboveground fresh weights of the test plots with different treatment amounts of tryptophan and reduced glutathione are shown as the change rate (%) relative to the aboveground fresh weight of the test plot (control plot) treated with a coating solution containing no tryptophan and reduced glutathione. Fig.15 .

[0224] according to Fig.15 Under salt stress conditions, when only GSH (reduced glutathione) was applied to seeds, the change rate of the aboveground fresh weight 3 weeks after sowing was 1% relative to no treatment, and when only GSH was applied to seeds, it was 5% to 10% relative to no treatment. When GSH and tryptophan were combined, it was 3% to 7%, and no synergistic effect was observed due to the combination of GSH and tryptophan.

[0225] <Example 8>

[0226] Corn field trials

[0227] Preparation of coated seeds

[0228] Corn seeds coated with 0.25 g of tryptophan per ton of seeds, corn seeds coated with 75 g of oxidized glutathione per ton of seeds, and corn seeds coated with 0.25 g of tryptophan and 75 g of oxidized glutathione per ton of seeds were prepared by the same method as in Example 1. Seeds not subjected to coating treatment were prepared as a control.

[0229] Field cultivation

[0230] Location: Fields of Wilderen MAR, Kingdom of Belgium

[0231] Type: Like IT

[0232] Planting date: April 27, 2020

[0233] Payoff date: October 12, 2020

[0234] Cultivation was carried out through routine fertilization management and pest and disease management, and a yield survey was conducted.

[0235] Yield survey: 120 plants / area, repeated 4 times

[0236] result

[0237] The ratios (%) of the ear yields of corn grown from the three types of coated seeds are shown in Table 1, with the ear yield of corn grown from uncoated seeds in the control plot set as 100%. Fig.16 .

[0238] according to Fig.16 , seeds coated with 0.25g of tryptophan, 75g of GSSG, and a combination of 0.25g of tryptophan and 75g of GSSG per ton of seeds were sown in a field and cultivated until harvest, and an investigation was conducted. Compared with the untreated area, the female ear yield in the case of tryptophan alone was 100.4%, and in the case of GSSG alone it was 100.7%, and no increase in yield was observed. On the other hand, in the case of the combination of tryptophan and GSSG, the female ear yield in the untreated area was 106.1%, showing an unexpected synergistic effect of an increase of 6.1%, and the effect of increasing the final yield brought about by the seed treatment was confirmed.

[0239] <Example 9>

[0240] Wheat cultivation from coated seeds under low temperature stress conditions

[0241] A coating treatment was carried out by coating 100 g of wheat seeds (variety: Yumechikara) with 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and coloring pigment in water using a coating device (manufactured by SATEC). The coating treatment was carried out individually or in combination with 5 g / MT-seed of tryptophan and 75 g / MT-seed of oxidized glutathione. The soil was filled in a 7.5 cm pot, and 1 grain of wheat seed after the coating treatment was sown / pot. The seeds were cultivated in a glass greenhouse until germination, and after germination, they were transferred to an artificial climate chamber set at 10°C for 12 hours during the day and 5°C for 12 hours at night for cultivation. The investigation was carried out 6 weeks after sowing. In the investigation, the dry weight of the aboveground part and the dry weight of the underground part were measured. The test was repeated 8 times and the average value was calculated.

[0242] The results are shown in Table 1. The "%" in the table represents a conversion value when the weight under the untreated condition is set to 100%. Although no initial growth promoting effect was confirmed in the case of tryptophan alone, a certain initial growth promoting effect was confirmed in the case of glutathione alone. In addition, by combining glutathione with tryptophan, an increase of 17% in the aboveground dry weight and 24% in the underground dry weight was observed relative to no treatment, both of which increased significantly. Thus, it was confirmed that glutathione and tryptophan showed a synergistic effect on growth promotion. As described above, it can be confirmed that the combination of glutathione and tryptophan shows a stable growth promoting effect even under low temperature stress conditions.

[0243]

【surface"

[0244]

[0245] <Example 10>

[0246] Cultivation of corn under dry stress conditions starting with seed coating

[0247] <Method>

[0248] A coating treatment was carried out by coating 800 μL of a coating solution containing tryptophan, oxidized glutathione, polyvinyl alcohol, and coloring pigment in water on 100 g of dent corn seeds (variety: Snow-dent Otoha) using a coating device (manufactured by SATEC). The coating treatment was carried out individually or in combination with 0.25 g / MT-seed of tryptophan and 75 g / MT-seed of oxidized glutathione. The 12 cm pot was filled with soil, and 7 dent corn seeds / pot after the coating treatment were sown. The seeds were cultivated in a glass greenhouse, and watering was stopped for 9 days after 1 week of sowing to make them dry, and then watering was resumed, and the seeds were cultivated for 4 days and investigated. The test was repeated 3 times to obtain the average value.

[0249] The results are shown in Table 2. The "%" in the table represents the conversion value when the weight under the untreated condition is set to 100%. The aboveground dry weight and underground dry weight of the test area treated with the combination of glutathione and tryptophan increased by 10% and 7%, respectively, compared with the untreated condition. Thus, it can be confirmed that the combination of glutathione and tryptophan shows a stable growth-promoting effect even under low temperature stress conditions.

[0250]

Table 2

[0251]

[0252] All publications, patents and patent applications cited in this specification are incorporated herein by direct reference.

Claims

1. A composition for coating plant seeds, comprising glutathione, amino acids, and an agriculturally acceptable carrier.

2. The composition according to claim 1, wherein The glutathione is oxidized glutathione.

3. The composition according to claim 1 or 2, wherein The amino acid is L-tryptophan.

4. The composition according to any one of claims 1 to 3, wherein The amino acid is contained in an amount of 0.012 parts by weight or more and 167 parts by weight or less relative to 100 parts by weight of the glutathione.

5. The composition according to any one of claims 1 to 4, wherein The composition contains 3 g or more of glutathione in an amount for coating 1 ton of plant seeds. 6 . The composition according to claim 1 , which is used for promoting initial growth of a plant.

7. The composition according to any one of claims 1 to 6, which is used for promoting the growth of plants under stress conditions.

8. The composition according to any one of claims 1 to 7, for use in increasing the yield of plants.

9. A coated seed comprising: Plant seeds, and A composition comprising glutathione and amino acids is present on the surface of the seeds.

10. The coated seed according to claim 9, wherein The glutathione is oxidized glutathione.

11. The coated seed according to claim 9 or 10, wherein The amino acid is L-tryptophan.

12. The coated seed according to any one of claims 9 to 11, wherein The composition includes 0.012 parts by weight or more and 167 parts by weight or less of the amino acid relative to 100 parts by weight of the glutathione.

13. The coated seed according to any one of claims 9 to 12, wherein The coated seeds contain more than 3 g of glutathione per 1 ton.

14. A method for cultivating plants, the method comprising: sowing the coated seeds according to any one of claims 9 to 13, and The plants are grown from the coated seeds after sowing.

15. The method according to claim 14, wherein: The step of growing the plant includes promoting initial growth of the plant.

16. The method according to claim 14 or 15, wherein: The step of growing the plant comprises growing the plant under stress conditions.

17. A composition for promoting initial growth of a plant, comprising glutathione and 0.012 parts by weight or more and 167 parts by weight or less of an amino acid based on 100 parts by weight of the glutathione.

18. A composition for promoting plant growth under stress conditions, comprising: Glutathione, and The amino acid is contained in an amount of 0.012 parts by weight or more and 167 parts by weight or less relative to 100 parts by weight of the glutathione.

19. A composition for increasing plant yield, comprising: Glutathione, and The amino acid is contained in an amount of 0.012 parts by weight or more and 167 parts by weight or less relative to 100 parts by weight of the glutathione.

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