Device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time

Abstract

The invention relates to a device and a method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time. The invention relates to a device and a method for automatically extracting Coleman-fat, Nanofat and SVF-gel. Comprising a pair of discs, two linear push rod assemblies, two sets of injector assemblies, a set of connecting pipes and the like. In the first set of injector assembly, an original fat solution is filled into a first injector, a rough filtration suite is mounted at an outlet of the first injector, a three-way piece is mounted at an outlet of a second injector, and a fine filtration suite is mounted at an outlet of a third injector; in the second set of injector assembly, the original fat solution is changed into a counterweight solution, other parts are the same as those of the first set of injector assembly, and the two sets of injector assemblies are arranged in a disc; the method comprises the following steps: carrying out first-time centrifugation on fat liquid in a first injector, carrying out second-time centrifugation on fat liquid in a second injector, carrying out first-time centrifugation to obtain Coleman-fat, pushing the layered fat tissue into the second injector by a linear push rod assembly, then mutually matching the two linear push rod assemblies to push the fat liquid in the first injector and the second injector to flow back and forth, emulsifying the fat to obtain Nanofat, and carrying out second-time centrifugation to obtain SVF-gel in a third injector.

Description

Technical Field

[0001] The invention relates to a device and a method for extracting Coleman-fat, Nanofat and SVF-gel from adipose tissue, in particular to a device and a method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time. Background Art

[0002] Stem cells are primitive cells with the ability to self-replicate and multidirectionally differentiate. Stem cells can produce any cell type, such as cartilage cells, nerve cells, bone cells, and fat cells. Stem cells exist in umbilical cord blood, umbilical cord, bone marrow, and adipose tissue, especially in the stromal vascular fraction (SVF) of adipose tissue, which is sometimes also called matrix (or interstitial) vascular fragments. The presence of stem cells in adipose tissue improves the effectiveness of transplantation in conservative surgery, regenerative surgery, and tissue reconstruction. Because adipose tissue is easy to extract, it is often used as a transplant material for beauty and medical purposes.

[0003] The original adipose tissue extracted from the donor contains not only adipose tissue, but also blood and swelling fluid. The cell tissue contains adipocytes and reticular extracellular matrix (ECM), which is also called intercellular matrix. The conventional method for extracting Coleman-fat, Nanofat and SVF-gel from the original adipose tissue is as follows:

[0004] Extraction of Coleman-fat: extract original adipose tissue from the donor, cover the outlet of the syringe containing such adipose tissue with a Luer cap, break the piston shaft at the back of the syringe, put it into a centrifuge (you can also transfer such original adipose tissue to a centrifuge tube), put it into the centrifuge; run the centrifuge at 1200g×3min. Under the action of centrifugal force, due to the difference in density of different substances, the adipose tissue and blood and swelling fluid in the original adipose tissue are separated into layers. Remove the blood and swelling fluid layers. The fat obtained in this way is called Coleman-fat.

[0005] Extraction of Nanofat: Coleman-fat is transferred to a syringe, and the outlet of the syringe is connected to the outlet of a filter device. The filter device has two outlets, and the other outlet is connected to another empty syringe. A filter screen (knife) is installed inside the filter device. The size of the filter screen (knife) is changed according to the purpose of the fat. If there is no filter screen (knife) inside the filter device, it only serves as a connection. The fat flows back and forth between the two syringes at 10ml / sec×1min. The emulsified fat is called nanofat.

[0006] Extraction of SVF-gel: Push the nanofat into a syringe, cover the outlet with a Luer cap, break the piston shaft at the back of the syringe, put it into a centrifuge, and run the centrifuge at 2000g×3min. Under the action of centrifugal force, the oil, ECM / SVF-gel, blood and swelling fluid in the nanofat are separated into layers. The uppermost layer of oil and the lowermost layer of blood and swelling fluid are removed, and the fat tissue in the middle is the SVF-gel.

[0007] Collagenase can digest the connected parts in adipose tissue to make it a single cell. In the process of extracting Coleman-fat, Nanofat and SVF-gel, in order to improve the efficiency of obtaining cells, collagenase can be added to the adipose tissue in the syringe or centrifuge tube, or collagenase can be omitted. The disadvantage of adding collagenase is that it increases the risk of cell contamination.

[0008] The disadvantages of these extraction methods of Coleman-fat, Nanofat and SVF-gel are that during the extraction process, the operator is required to complete each step step by step. Due to the repeated disassembly and assembly of medical devices and the repeated transfer of fat materials, the fat tissue is repeatedly in contact with the surfaces of various devices and with the air in a non-perfectly sterilized environment, which increases the risk of bacterial or viral contamination.

[0009] Patents US2007 / 0274960, WO2009 / 073724 and CN103038333 provide several methods and devices for extracting fat cells from adipose tissue. However, since these methods are not completed automatically in one machine at one time, operators are required to participate in some of the processes or steps, and the risk of bacterial or viral contamination is still relatively high. Currently, there is a lack of a method and device for automatically extracting Coleman-fat, Nanofat and SVF-gel in one machine at one time. Summary of the invention

[0010] In order to minimize the risk of bacterial or viral contamination during the extraction of Coleman-fat, Nanofat and SVF-gel, the present invention provides a device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time. The method is simple in principle and easy to implement.

[0011] The technical solution adopted by the present invention to solve its technical problems is: the device for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time comprises a pair of discs, a fixing cap, a driving motor, a set of valve switch components, two linear push rod components, two sets of syringe components, a set of connecting pipes and two sets of photoelectric sensors. The fixing cap fixes the pair of discs on the driving motor, and the driving motor controls the rotation and positioning of the discs; the valve switch component comprises a linear motion motor, a guide rail slider and a rotary motor, and the linear motor drives the slider to perform linear motion on the guide rail, and the rotary motor drives the slider to perform linear motion on the guide rail. The rotary motor is installed on the slider; each linear push rod assembly includes a driving motor and a set of mechanical mechanisms, and the two linear push rod assemblies are used to push the fat liquid in the two syringes to flow back and forth; a pair of discs includes an upper disc and a lower disc, and the lower disc has grooves in it, and the grooves are arranged symmetrically. The structure of the upper disc is divided into two types: with grooves and without grooves. When the upper disc has grooves, the grooves of the upper disc are mirror-symmetrical with the grooves of the lower disc. The upper disc plays the role of a guard plate, and also plays a role of fixing and positioning. When the upper disc has no grooves, it acts as a guard plate, and the diameter of the disc is 400-600mm. Two sets of syringe assemblies and a set of connecting pipes are connected and installed in the grooves inside a pair of discs, and a set of valve switch assemblies are installed below the discs. The linear motion push rods of the two linear push rod assemblies are at the same horizontal position as the syringes placed in the discs, and the angles of the linear motion push rods of the two linear push rod assemblies are equal to the angles of the syringes.The first set of syringe assemblies includes four syringes, a coarse filter kit, a fine filter kit, and at least one three-way piece. The first three-way piece is one of a three-way and a three-way valve, and the other three-way pieces are three-way valves. The coarse filter kit is installed at the outlet of the first syringe, the first three-way piece is installed at the outlet of the second syringe, and the fine filter kit is installed at the outlet of the third syringe. One of the second and third syringes is filled with 0-5ml of collagenase, and no collagenase is added when 0ml, and the other is an empty tube. The coarse filter kit includes a pair of shells that can be unscrewed, 1-3 level coarse filter screens, and 1 check plate. Each check plate and coarse filter screen is equipped with a gasket. The coarse filter screen, check plate and gasket are placed in the shell. The pore size of the 1-3 level coarse filter screen is between 0.4-2mm, and the check plate is installed inside the coarse filter kit , gasket and coarse filter, a space of 0.5-2mm should be left inside in the axial direction, when the first tee is a three-way valve, the check plate can be removed, when the first tee is a three-way, the check plate should be retained, the fine filter kit includes a pair of openable shells and 1-2 level fine filters, the pore size of the 1-2 level fine filters is between 0.2-1mm, the fine filter is placed in the shell, one port of the coarse filter kit is connected to a port of the first tee, one port of the first tee is connected to the fine filter kit, when the second tee is installed at the outlet of the third syringe, one port of the second tee is connected to the fourth syringe, the fourth syringe is an empty tube, when the second tee and the fourth syringe are not installed, one outlet of the first tee is directly connected to the fine filter kit at the outlet of the third syringe. The first group of photoelectric sensors is installed under the disc. When the lower disc rotates, the part of the first syringe near the outlet passes over the first group of photoelectric sensors, and the part of the third syringe near the outlet passes over the second group of photoelectric sensors. At least one of the syringe and the connecting tube is transparent so that the photoelectric sensor can measure the color change of the internal fluid.

[0012] The second set of syringe assemblies has the same structure as the first set of syringe assemblies, except that the collagenase is replaced with other solutions of the same volume and similar density. The second set of syringe assemblies is installed in the groove of the lower disk, and the first set of syringe assemblies and the second set of syringe assemblies are placed symmetrically in the groove of the disk.

[0013] A method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time is provided. The original fat solution containing fat, blood and swelling fluid is extracted from the patient's body. All the coarse filtration kits, fine filtration kits, syringes and three-way fittings are connected with connecting tubes and placed in the groove of the lower disc; the first syringe of the first syringe assembly is filled with the original fat solution, and the pistons of the second and third syringes are pushed to the end closest to the outlet; the first syringe of the second syringe assembly is filled with a weight solution of the same volume and similar density, and the pistons of the second and third syringes are pushed to the end closest to the outlet; the first centrifugation is performed at 1200g×3min. After the centrifugation is completed, the disc is in a vertical position, the first syringe of the first syringe assembly is at the bottom, the fat layer is at the top of the first syringe, and the blood and swelling fluid layer are at the bottom of the first syringe, and the second syringe assembly is opened. A three-way valve pushes the fat tissue layer in the first syringe of the first set of syringe components into the second syringe. The first group of photoelectric sensors detects the interface between the fat layer and the blood and swelling fluid layer and records the position. After the fat layer is pushed into the second syringe, the first three-way valve is closed to obtain Coleman-fat in the second syringe. If Nanofat needs to be extracted, continue the following process; the first linear push rod assembly and the second linear push rod assembly push the piston shafts of the second syringe and the third syringe back and forth at 10ml / sec×1min to complete fat emulsification and obtain Nanofat in the third syringe. If SVF-gel needs to be extracted, continue the following process; the second set of syringe components repeats all the above actions and distances except the fat emulsification action, and centrifuges for the second time at 2000g×3min. After the centrifugation is completed, SVF-gel is obtained in the third syringe of the first set of syringe components.

[0014] The specific operation process is as follows: cover the upper disc, and use the fixing cap to press and fasten the upper and lower discs together. The driving motor rotates, and the first centrifugation is 1200g×3min. Under the action of centrifugal force, fat, blood and swelling fluid are layered due to different densities. The driving motor slows down and stops the first syringe above the position of the first group of photoelectric sensors. The first linear push rod assembly moves forward, and the fat at the front end of the first syringe near the outlet is pushed into the second syringe through the coarse filtration kit and the first three-way piece to obtain Coleman-fat; the distance moved by the first linear push rod assembly is recorded so that the second set of syringes can complete the same distance, and then the first linear push rod retreats, and then the driving motor rotates to align the second syringe in the disc with the first linear push rod assembly, and the third syringe with the second linear push rod assembly. The first linear push rod assembly moves forward, and the fat in the second syringe is pushed into the third syringe through the fine filtration kit. The first linear push rod assembly retreats, and at the same time, the second linear push rod assembly moves forward, and the fat in the third syringe is pushed back to the second syringe through the fine filtration kit. The second linear push rod assembly retreats, and the first linear push rod assembly moves forward. This process is reciprocating, 10ml / sec×1mi n, the first linear push rod assembly retreats, the second linear push rod assembly retreats, the fat is emulsified to obtain nanofat; if there is collagenase in the syringe, the single cell acquisition process can be accelerated; the driving motor rotates a pair of disks to the position of the second set of syringe assemblies, except that the emulsification process is not performed, and the above actions are completely repeated to ensure that the center of gravity of the first set of syringe assemblies and the center of gravity of the second set of syringe assemblies are basically symmetrical with the axis of the driving motor; the driving motor drives a pair of disks to rotate, 2000g×3min, under the action of centrifugal force, the SVF-gel, oil and residual liquid in the emulsified fat tissue are layered due to different densities to obtain SVF-gel; when the SVF needs to be placed in the fourth syringe alone, first use the second linear push rod assembly to push the oil closest to the outlet of the third syringe into the second syringe, the valve switch assembly rises to open the second three-way valve, the third syringe is connected to the fourth syringe, the valve switch assembly descends and resets, and the second linear push rod assembly pushes the SVF closest to the outlet of the third syringe into the fourth syringe, so that the fourth syringe only has SVF-gel.

[0015] The beneficial effect of the present invention is to automatically extract Coleman-fat, Nanofat and SVF-gel at one time without the need for multiple manual operations, thereby minimizing the risk of bacterial or viral contamination during the extraction of Coleman-fat, Nanofat and SVF-gel. This can be achieved because the technical measures adopted by the present invention are mature and feasible. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0017] Figure 1 It is a preferred embodiment of the disc of the present invention.

[0018] Figure 2 is another preferred embodiment of the disc of the present invention.

[0019] Figure 3 It is a preferred embodiment of the internal check plate, gasket and coarse filter screen of the coarse filter kit of the present invention.

[0020] Figure 4 It is a preferred embodiment of the outer shell on the side close to the check plate in the coarse filter kit of the present invention.

[0021] Figure 5 It is a preferred embodiment of the relative position of the disc and the linear push rod assembly of the present invention.

[0022] In the figure, 1. syringe, 2. syringe, 3. syringe, 4. syringe, 5. coarse filter kit, 6. connecting pipe, 7. three-way valve, 8. connecting pipe, 9. fine filter kit, 10. photoelectric hole, 11. syringe, 12. syringe, 13. syringe, 14. syringe, 15. coarse filter kit, 16. connecting pipe, 17. three-way valve, 18. connecting pipe, 19. fine filter kit, 20. photoelectric hole, 21. disk, 22. process tank, 23. connecting pipe, 24. three-way, 25. three-way valve, 26. photoelectric hole, 27. connecting pipe, 28. three-way, 29. three-way valve, 30. photoelectric hole, 31. check plate, 32. gasket, 33. coarse filter screen, 34. gasket, 35. coarse filter screen, 36. gasket, 37. coarse filter screen, 38. Gasket, 39. Housing, 40. Inner hole, 41. Cavity, 42. Linear push rod assembly, 43. Linear motor, 44. Push rod, 45. Guide rail, 46. Slider, 47. Linear push rod assembly, 48. Linear motor, 49. Push rod, 50. Guide rail, 51. Slider, 52. Upper disc. DETAILED DESCRIPTION

[0023] exist Figure 1In the embodiment, a disk (21) is provided with a process groove (22), a photoelectric hole (10), a photoelectric hole (20) and two sets of syringe assemblies. The process groove (22) is a non-transparent groove processed to reduce the inertia of the disk (21). The first set of syringe assemblies includes a syringe (1), a syringe (2), a syringe (3), a coarse filter kit (5), a connecting pipe (6), a three-way valve (7), a connecting pipe (8) and a fine filter kit (9); the second set of syringe assemblies includes a syringe (11), a syringe (12), a syringe (13), a coarse filter kit (15), a connecting pipe (16), a three-way valve (17), a connecting pipe (18) and a fine filter kit (19); the two sets of syringe assemblies are installed in the groove in the disk (21). The manufacturing material of the syringe (1), the syringe (2) and the syringe (3) is transparent medical plastic. The piston shaft behind the piston inside the syringe can be broken at the thinnest part. Before the syringe (1), the syringe (2) and the syringe (3) are placed in the disk (21), the piston shaft is broken.

[0024] exist Figure 2 In the invention, a disk (21) is provided with a process groove (22), a photoelectric hole (10), a photoelectric hole (20), a photoelectric hole (26), a photoelectric hole (30) and two sets of syringe assemblies. The process groove (22) is a non-transparent groove processed to reduce the inertia of the disk (21). The first set of syringe assemblies includes a syringe (1), a syringe (2), a syringe (3), a syringe (4), a coarse filter kit (5), a connecting pipe (6), a three-way valve (7), a connecting pipe (8), a fine filter kit (9), a connecting pipe (23), a three-way valve (24) and a three-way valve (25); the second set of syringe assemblies includes a syringe (11), a syringe (12), a syringe (13), a syringe (14), a coarse filter kit (15), a connecting pipe (16), a three-way valve (17), a connecting pipe (18), a fine filter kit (19), a connecting pipe (27), a three-way valve (28) and a three-way valve (29); the two sets of syringe assemblies are installed in the groove in the disk (21).

[0025] exist Figure 3 In the coarse filter kit, the check plate (31), the gasket (32), the coarse filter screen (33), the gasket (34), the coarse filter screen (35), the gasket (36), the coarse filter screen (37), and the gasket (38) are arranged such that the filter hole size of the coarse filter screen (33) is larger than the filter hole size of the coarse filter screen (35), the filter hole size of the coarse filter screen (35) is larger than the filter hole size of the coarse filter screen (37), and the outer diameters of the check plate (31), the gasket (32), the coarse filter screen (33), the gasket (34), the coarse filter screen (35), the gasket (36), the coarse filter screen (37), and the gasket (38) are preferably equal.

[0026] exist Figure 4In the embodiment, the outer shell (39) of the coarse filter kit near the side of the check plate has an inner hole (40) with a diameter smaller than the diameter of the central disc of the check plate (31), so that the fat tissue can only flow from the check plate (31) to the coarse filter screen (33), the coarse filter screen (35) and the coarse filter screen (37) behind it, but cannot flow in the opposite direction. The diameter of the cavity (41) is 0.15-1.5 mm larger than the outer diameter of the check plate (31).

[0027] exist Figure 5 In the embodiment, the linear push rod assembly (42) includes a linear motor (43), a push rod (44), a guide rail (45) and a slider (46), the linear push rod assembly (47) includes a linear motor (48), a push rod (49), a guide rail (50) and a slider (51), and the upper disc (52) covers the disc (21). No collagenase is placed in the syringe (1) and the syringe (2). The driving motor drives the disc (21) and the upper disc (52) to rotate, 1200g×3min, after centrifugation, the original fat solution is separated into layers, the three-way valve (7) is opened, and the oil and fat layer closest to the outlet in the syringe (1) is pushed into the syringe (2) through the coarse filter kit (5) by the linear push rod assembly (42), and Coleman-fat is obtained in the syringe (2); the push rod (44) on the linear push rod assembly (42) and the push rod (49) on the linear push rod assembly (47) cooperate with each other and reciprocate, and the fat tissue in the syringe (2) and the syringe (3) flows back and forth through the fine filter kit (9), 10ml / sec×1min, and the fat tissue can be emulsified, and Nanofat can be obtained in the syringe (3). Through the back and forth rotation of the disc (21), even if only one of the linear push rod assembly (42) and the linear push rod assembly (47) is retained, the above emulsification process can be completed, and the work efficiency will be reduced. After the above process is completed, the driving motor drives the disc (21) and the upper disc (52) to rotate at 1200g×3min. The SVF-gel, oil and residual liquid are separated into layers due to their different densities, thereby obtaining SVF-gel.

[0028] Those skilled in the art should recognize that, although only five embodiments are described above, they are not all forms of the present invention. It should be understood that many modifications can be made without departing from the spirit and scope of the present invention. Those skilled in the art can implement the present invention in other forms without departing from the concept of the present invention. Therefore, other embodiments are also within the scope of the claims of the present invention.

Claims

1. A device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time, comprising a pair of discs, a fixing cap, a driving motor, a set of valve switch components, two linear push rod components, two sets of syringe components, a set of connecting tubes and two sets of photoelectric sensors; the fixing cap fixes the pair of discs on the driving motor, and the driving motor controls the rotation and positioning of the discs; the valve switch component comprises a linear motion motor, a guide rail slider and a rotary motor, the linear motor drives the slider to perform linear motion on the guide rail, the rotary motor is installed on the slider, each linear push rod component comprises a driving motor and a set of mechanical mechanisms, and is characterized in that: A pair of discs comprises an upper disc and a lower disc, the lower disc has grooves in it, the grooves are arranged symmetrically, the upper disc has a structure with grooves and without grooves, when the upper disc has grooves, the grooves of the upper disc are mirror-symmetrical with the grooves of the lower disc, the upper disc acts as a guard plate, and also plays a role of fixing and positioning, when the upper disc has no grooves, it acts as a guard plate, and the disc diameter is 400-600mm; two sets of syringe assemblies and a set of connecting pipes are connected and installed in the grooves inside the pair of discs, a set of valve switch assemblies is installed below the discs, the linear motion push rods of the two linear push rod assemblies are at the same horizontal position as the syringes placed in the discs, and the angles of the linear motion push rods of the two linear push rod assemblies are equal to the angles of the syringes.

2. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 1, characterized in that: The first set of syringe assemblies includes four syringes, a coarse filtration kit, a fine filtration kit, and at least one three-way piece. The first three-way piece is one of a three-way and a three-way valve, and the other three-way pieces are three-way valves. The coarse filtration kit is installed at the outlet of the first syringe, the first three-way piece is installed at the outlet of the second syringe, and the fine filtration kit is installed at the outlet of the third syringe; one of the second syringe and the third syringe is filled with 0-5 ml of collagenase, no collagenase is added when 0 ml, and the other is an empty tube.

3. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 1, characterized in that: The coarse filter kit includes a pair of unscrewed shells, 1-3 level coarse filter screens, and a check plate. Each check plate and coarse filter screen is equipped with a gasket. The coarse filter screen, check plate and gasket are placed in the shell. The aperture of the 1-3 level coarse filter screen is between 0.4-2mm. After the check plate, gasket and coarse filter screen are installed in the coarse filter kit, a space of 0.5-2mm should be left in the axial direction; when the first three-way piece is a three-way valve, the above-mentioned check plate can be removed. When the first three-way piece is a three-way, the check plate is retained; the fine filter kit includes a pair of openable shells and 1-2 level fine filter screens. The aperture of the 1-2 level fine filter screens is between 0.2-1mm, and the fine filter screen is placed in the shell.

4. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 1, characterized in that: One port of the coarse filtration kit is connected to one port of the first tee piece, one port of the first tee piece is connected to the fine filtration kit, when the second tee piece is installed at the outlet of the third syringe, one port of the second tee piece is connected to the fourth syringe, the fourth syringe is an empty tube, when the second tee piece and the fourth syringe are not installed, one outlet of the first tee piece is directly connected to the fine filtration kit at the outlet of the third syringe.

5. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 1, characterized in that: The first group of photoelectric sensors is installed under the disc. When the lower disc rotates, the part of the first syringe near the outlet passes over the first group of photoelectric sensors, and the part of the third syringe near the outlet passes over the second group of photoelectric sensors. At least one of the syringe and the connecting tube is transparent so that the photoelectric sensor can measure the color change of the internal fluid.

6. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 1, characterized in that: The second set of syringe assemblies has the same structure as the first set of syringe assemblies, except that the collagenase is replaced with other solutions of the same volume and similar density. The second set of syringe assemblies is installed in the groove of the lower disk, and the first set of syringe assemblies and the second set of syringe assemblies are placed symmetrically in the groove of the disk.

7. A device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time, wherein all the coarse filtration kits, fine filtration kits, syringes and three-way fittings are connected with a connecting pipe and placed in the groove of the lower disc, wherein the device and method are characterized by: The first syringe of the first set of syringe components is filled with the original fat solution, and the pistons of the second and third syringes are pushed to the end closest to the outlet; the first syringe of the second set of syringe components is filled with a weight solution of equal volume and similar density, and the pistons of the second and third syringes are pushed to the end closest to the outlet; after the first centrifugation, the first three-way valve is opened, the fat tissue layer in the first syringe of the first set of syringe components is pushed into the second syringe, and the first three-way valve is closed to obtain Coleman-fat in the second syringe; then the first linear push rod assembly and the second linear push rod assembly push the piston shafts of the second and third syringes back and forth to complete fat emulsification, and obtain Nanofat in the third syringe; the second set of syringe components repeats all the above actions except the fat emulsification action, and after the second centrifugation, SVF-gel is obtained in one of the second and third syringes of the first set of syringe components.

8. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 7, characterized in that: After the first centrifugation, the driving motor decelerates and stops the first syringe above the position of the first group of photoelectric sensors, the first linear push rod assembly moves forward, and the oil and fat at the front end of the first syringe near the outlet are pushed into the second syringe through the coarse filter kit and the first tee piece, the movement distance of the first linear push rod assembly is recorded, the first linear push rod retreats, the driving motor rotates to align the second syringe in the disc with the first linear push rod assembly, the third syringe is aligned with the second linear push rod assembly, the first linear push rod assembly moves forward, the oil and fat in the second syringe are pushed into the third syringe through the fine filter kit, the first linear push rod assembly retreats, and the second linear push rod assembly moves forward at the same time, the oil and fat in the third syringe are pushed back to the second syringe through the fine filter kit, the second linear push rod assembly retreats, and the first linear push rod assembly moves forward to emulsify the fat.

9. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 7, characterized in that: The driving motor rotates the disc to the position of the second set of syringe assemblies, and completely repeats the above actions except that the fat emulsification process is not performed to ensure that the center of gravity of the first set of syringe assemblies and the center of gravity of the second set of syringe assemblies are basically symmetrical with the axis of the driving motor.

10. The device and method for automatically extracting Coleman-fat, Nanofat and SVF-gel at one time according to claim 7, characterized in that: The driving motor drives a pair of discs to rotate, and the second centrifugation is performed. The SVF-gel, oil and residual liquid in the fat tissue are separated into layers to obtain SVF-gel. When the SVF-gel needs to be placed in the fourth syringe alone, the oil closest to the outlet of the third syringe is first pushed into the second syringe by the second linear push rod assembly. The valve switch assembly rises to open the second three-way valve, and the third syringe is connected to the fourth syringe. The valve switch assembly retreats, and the second linear push rod assembly pushes the SVF closest to the outlet of the third syringe into the fourth syringe. In this way, there is only SVF-gel in the fourth syringe.

Images