Skin repair liquid dressing as well as preparation method and application thereof

Abstract

The invention discloses a skin repair liquid dressing, which is prepared by reasonably proportioning carbomer, hyaluronic acid, glycerol, tridecapeptide, allantoin, nano-scale beta-tricalcium phosphate, a natural polysaccharide mixture and purified water, and the prepared skin repair liquid dressing has the advantages of fast film forming time, good repair effect on skin injury, fast skin healing speed, high repair efficiency, no toxic or side effect, and no toxic or side effect. And the traditional Chinese medicine has a good antibacterial effect, and can prevent early-stage pigmentation after wound healing and reduce scars. Wherein the natural polysaccharide mixture is prepared from pomegranate flower polysaccharide, panax japonicus polysaccharide and polygonatum cyrtonema polysaccharide according to a reasonable ratio, and can achieve a synergistic effect to remarkably improve the repair efficiency and inhibit pigmentation and scar formation. The beta-tricalcium phosphate has excellent biocompatibility, biological activity and capability of promoting formation of new tissues, so that healing of wounds is accelerated, in addition, the nano-scale particles have larger specific surface areas, bioactive ions are favorably released, and the repair process is further promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of medical dressings, and in particular to a skin repair liquid dressing and a preparation method and application thereof. Background Art

[0002] The skin is the largest organ in the human body. It is in direct contact with the outside world. It prevents the loss of water, electrolytes and nutrients through physical, chemical and biological damage protection mechanisms, becoming the first barrier covering the surface of the human body. The structure of the skin consists of three layers. The outermost layer is the epidermis, followed by the dermis and subcutaneous tissue. Although the structure of the skin has given it a strong ability to resist external invasion, the complex and changeable external environment means that even the skin will still be damaged. Skin damage is very common in daily life, including mild, moderate and severe acne, dermatitis, eczema and other skin problems, as well as skin abrasions, mild burns, sunburn and mild trauma after medical aesthetics. These injuries can cause damage to the skin barrier function, reduce the skin's ability to resist external bacteria, and easily induce inflammatory reactions such as itching, redness, swelling and stinging. In some cases, the injury may also be accompanied by a small amount of bleeding and tissue fluid exudation. Medical dressings play a key role in this process, helping the skin barrier to recover by protecting the wound surface, preventing infection, promoting repair and accelerating healing. When the skin is damaged, the human body loses its barrier. It is crucial to repair the skin quickly and with high quality. Ideally, the skin healing process is a complex and orderly physiological reaction that covers four core stages: hemostasis, inflammatory response, cell proliferation, and tissue remodeling. However, due to various adverse external factors and the patient's own interference, wound repair cannot proceed perfectly according to this process, resulting in the formation of chronic wounds.

[0003] Chronic wounds usually face challenges such as high inflammation, easy infection and irregular wound surface, so it is particularly important to develop targeted medical dressings. Since the "moist healing" theory was proposed, liquid dressings have become the focus of wound dressing research due to their superior water retention and cytoplasmic matrix structure. Natural polymer polysaccharides are widely used in the development of skin wound dressings due to their natural sources, high biocompatibility and low cost. However, most of these dressings are still relatively simple in function and cannot meet the treatment needs of complex chronic wounds. Therefore, the present invention aims to design a liquid dressing specifically for skin repair by combining natural polysaccharides with other functional components, in order to provide new ideas and methods for solving the problem of chronic wounds being difficult to heal. Summary of the invention

[0004] In view of this, the present invention proposes a skin repair liquid dressing and a preparation method and application thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A skin repair liquid dressing comprises the following raw material components by weight percentage: 0.1%-1% carbomer, 0.05%-0.25% hyaluronic acid, 1%-5% glycerol, 0.05%-0.1% tridecapeptide, 0.1%-0.2% allantoin, 0.05%-0.2% nano-scale beta-tricalcium phosphate, 0.4%-0.8% natural polysaccharide mixture, and the balance purified water.

[0007] Preferably, the natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, Panax japonicus polysaccharide and Polygonatum cyrtonema polysaccharide.

[0008] Preferably, the mass ratio of the pomegranate flower polysaccharide, the panax japonicus polysaccharide and the polygonatum cyrtonema polysaccharide is: (2-4): (1-3): 1.

[0009] Preferably, the preparation method of the pomegranate flower polysaccharide is: extracting the dried pomegranate flowers with hot water, filtering, adding ethanol to the filtrate after concentrating the filtrate, standing and centrifuging to separate the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying to obtain the pomegranate flower polysaccharide; the hot water extraction conditions include: temperature control at 80-100°C, water-to-material ratio of 20-30:1 (mL / g), and extraction time of 2-3 hours.

[0010] More preferably, in the preparation process of the pomegranate flower polysaccharide, the hot water extraction conditions are: temperature 100° C., water-to-solid ratio 25:1 (mL / g), and extraction time 160 min.

[0011] Preferably, the preparation method of the polysaccharide of the bamboo joint is as follows: extract the dried bamboo joint ginseng by enzymatic hydrolysis with cellulase, filter, add ethanol to the filtrate after concentrating the filtrate, centrifuge after standing, separate the precipitate, deproteinize and depigment the precipitate, dialyze and freeze-dry to obtain the polysaccharide of the bamboo joint ginseng; the conditions for enzymatic hydrolysis with the cellulase include: enzyme dosage of 0.04-0.06g / g, enzymatic hydrolysis temperature of 40-50℃, water-to-material ratio of 30-50:1 (mL / g), and enzymatic hydrolysis time of 2-3 hours.

[0012] More preferably, in the preparation process of the Panax notoginseng polysaccharide, the conditions for cellulase hydrolysis are: enzyme dosage of 0.05 g / g, hydrolysis temperature of 45°C, water-to-material ratio of 40:1 (mL / g), and extraction time of 150 min.

[0013] Preferably, the preparation method of the Polygonatum cyrtonema polysaccharide is: extracting the dried Polygonatum cyrtonema with hot water, filtering, concentrating the filtrate, adding ethanol to the filtrate, standing and centrifuging to separate the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying to obtain the Polygonatum cyrtonema polysaccharide; the hot water extraction conditions include: temperature control at 65-80°C, water-to-material ratio of 30-40:1 (mL / g), and extraction time of 2-3 hours.

[0014] More preferably, in the preparation process of the Polygonatum cyrtonema, the hot water extraction conditions are: temperature 70° C., water-to-solid ratio of 35:1 (mL / g), and extraction time of 120 min.

[0015] Preferably, the average particle size of the nano-scale β-tricalcium phosphate is 100-600 nm.

[0016] More preferably, the average particle size of the nano-scale β-tricalcium phosphate is 300 nm.

[0017] The present invention also provides a method for preparing a skin repair liquid dressing, comprising the following steps:

[0018] S1. Take 60% to 80% of the total amount of purified water, slowly add carbomer, hyaluronic acid and glycerin in proportion, stir evenly, and let stand for 1 to 2 hours to allow it to fully soak and swell, to obtain premix A;

[0019] S2. Take 20% to 40% of the total amount of purified water, add the tridecapeptide and natural polysaccharide mixture in proportion, and stir at room temperature at a stirring speed of 100 to 150 r / min for 20 to 30 minutes to obtain a premixed solution B;

[0020] S3. Slowly pour the premixed liquid B into the container containing the premixed liquid A, stir evenly, then add allantoin and nano-scale β-tricalcium phosphate, stir evenly, and obtain a skin repair liquid dressing with uniform texture.

[0021] The present invention provides application of the skin repair liquid dressing in preparing skin antibacterial products.

[0022] The present invention provides application of the skin repair liquid dressing in preparing products for preventing pigmentation after skin wound healing, reducing scars and promoting wound healing.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] A skin repair liquid dressing provided by the present invention, carbomer, hyaluronic acid, glycerol, tridecapeptide, allantoin, nano-scale β-tricalcium phosphate, natural polysaccharide mixture, purified water are reasonably proportioned, and the prepared skin repair liquid dressing has a good repair effect on skin damage, fast skin healing speed, high repair efficiency, and good antibacterial effect, and can also prevent early pigmentation after wound healing and reduce scars. Wherein the natural polysaccharide mixture is composed of pomegranate flower polysaccharide, bamboo ginseng polysaccharide, and multi-flowered Polygonatum cyrtonema polysaccharide in a reasonable proportion, which can synergistically significantly improve the repair efficiency, shorten the healing time, and increase the proportion of good repair. Natural polysaccharides have excellent moisturizing and adhesion properties and anti-inflammatory effects, help to form a protective film, prevent wound drying and infection, accelerate wound healing, and inhibit scar formation. β-TCP has excellent biocompatibility, biological activity and the ability to promote the formation of new tissue, thereby accelerating the healing of the wound, and in addition, nano-scale particles have a larger specific surface area, which helps to release bioactive ions and further promote the repair process. In addition, based on the formula of the present invention, the preparation method of the present invention is adopted to obtain a skin repair liquid dressing product with stable effect, in which the effective ingredient natural polysaccharide mixture has higher activity. DETAILED DESCRIPTION

[0025] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0026] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0027] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0028] The preparation method of pomegranate flower polysaccharide is as follows:

[0029] S1. Crush the dried pomegranate flowers, add deionized water to the powder at a solid-liquid ratio of 25:1 (mL / g), extract at 100 degrees Celsius for 160 minutes, filter through gauze, and concentrate the filtrate;

[0030] S2. Add 3 volumes of 90% ethanol to the filtrate, let it stand at 5 degrees Celsius for 24 hours, and then centrifuge to separate the precipitate;

[0031] S3. Redissolve the precipitate, add 1 / 5 volume of Sevage solution (the mass ratio of chloroform to n-butanol is 4:1) to remove protein, shake on a shaker for 30 minutes, let it stand and centrifuge to remove the upper polysaccharide solution, stir it continuously in a fume hood for 20 minutes, let it stand for a while and then separate the layers (if no layers are separated, centrifuge again), take the supernatant and continue to add sevage to remove impurities, repeat several times until there is no protein layer;

[0032] S4. The solution after protein removal is passed through D101 macroporous adsorption resin, then rotary concentrated, dialyzed (molecular weight cutoff is 2000Da) and freeze-dried for storage to obtain pomegranate flower polysaccharide.

[0033] The preparation method of Panax japonicus polysaccharide is as follows:

[0034] S1. Grind the dried ginseng, put the ginseng powder in a container, add deionized water at a solid-liquid ratio of 1:40 g / mL, add cellulase at a ratio of 0.05 g cellulase / g ginseng, add a certain amount of citric acid / sodium citrate buffer solution (pH = 5.0), enzymolyze at 45 degrees Celsius for 2.5 hours, inactivate at 100 degrees Celsius for 2 minutes, filter, and concentrate the filtrate;

[0035] S2. Add 4 times the volume of 90% ethanol to the filtrate, let it stand at room temperature for 24 hours, and then centrifuge to separate the precipitate;

[0036] S3. Redissolve the precipitate, add 1 / 5 volume of Sevage solution (the mass ratio of chloroform to n-butanol is 4:1) to remove protein, shake on a shaker for 30 minutes, let it stand and centrifuge to remove the upper polysaccharide solution, stir it continuously in a fume hood for 20 minutes, let it stand for a while and then separate the layers (if no layers are separated, centrifuge again), take the supernatant and continue to add sevage to remove impurities, repeat several times until there is no protein layer;

[0037] S4. The solution after protein removal is passed through D101 macroporous adsorption resin, then rotary concentrated, dialyzed (with a molecular weight cutoff of 1000Da), and freeze-dried for storage to obtain Panax japonicus polysaccharide.

[0038] The preparation method of Polygonatum cyrtonema polysaccharide is as follows:

[0039] S1. Grind the dried Polygonatum cyrtonema, add deionized water to the powder at a solid-liquid ratio of 1:35, extract at 70 degrees Celsius for 120 minutes, filter with gauze, and concentrate the filtrate;

[0040] S2. Add 4 times the volume of 90% ethanol to the filtrate, let it stand at 5 degrees Celsius for 24 hours, and then centrifuge to separate the precipitate;

[0041] S3. Redissolve the precipitate, add 1 / 5 volume of Sevage solution (the mass ratio of chloroform to n-butanol is 4:1) to remove protein, shake on a shaker for 30 minutes, let it stand and centrifuge to remove the upper polysaccharide solution, stir it continuously in a fume hood for 20 minutes, let it stand for a while and then separate the layers (if no layers are separated, centrifuge again), take the supernatant and continue to add sevage to remove impurities, repeat several times until there is no protein layer;

[0042] S4. The solution after protein removal is passed through D101 macroporous adsorption resin, then rotary concentrated, dialyzed (molecular weight cutoff is 3000Da), and then freeze-dried and stored to obtain Polygonatum cyrtonema polysaccharide.

[0043] Example 1

[0044] A skin repair liquid dressing, comprising the following raw material components by weight percentage: 0.1% carbomer, 0.25% hyaluronic acid, 1% glycerol, 0.05% tridecapeptide, 0.2% allantoin, 0.05% nano-scale β-tricalcium phosphate, 0.4% natural polysaccharide mixture, and the balance purified water. The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, Panax notoginseng polysaccharide, and Polygonatum cyrtonema polysaccharide; the mass ratio of pomegranate flower polysaccharide, Panax notoginseng polysaccharide, and Polygonatum cyrtonema polysaccharide is 2:1:1; the average particle size of nano-scale β-tricalcium phosphate is 300nm.

[0045] The present invention also provides a method for preparing a skin repair liquid dressing, comprising the following steps:

[0046] S1. Take 80% of the total amount of purified water, slowly add carbomer, hyaluronic acid and glycerin in proportion, stir evenly, and let stand for 1 hour to allow it to fully soak and swell, to obtain premix A;

[0047] S2. Take 20% of the total amount of purified water, add the tridecapeptide and natural polysaccharide mixture in proportion, and stir at room temperature at a stirring speed of 100 r / min for 30 minutes to obtain a premixed solution B;

[0048] S3. Slowly pour the premixed liquid B into the container containing the premixed liquid A, stir evenly, then add allantoin and hydroxyapatite, stir evenly, and obtain a skin repair liquid dressing with a uniform texture.

[0049] Example 2

[0050] A skin repair liquid dressing, comprising the following raw material components by weight percentage: 0.5% carbomer, 0.2% hyaluronic acid, 3% glycerol, 0.08% tridecapeptide, 0.15% allantoin, 0.15% nano-scale β-tricalcium phosphate, 0.6% natural polysaccharide mixture, and the balance purified water. The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, Panax notoginseng polysaccharide, and Polygonatum cyrtonema polysaccharide; the mass ratio of pomegranate flower polysaccharide, Panax notoginseng polysaccharide, and Polygonatum cyrtonema polysaccharide is 3:2:1; the average particle size of nano-scale β-tricalcium phosphate is 300nm.

[0051] The present invention also provides a method for preparing a skin repair liquid dressing, comprising the following steps:

[0052] S1. Take 60% of the total amount of purified water, slowly add carbomer, hyaluronic acid and glycerin in proportion, stir evenly, and let stand for 2 hours to allow it to fully soak and swell, to obtain premix A;

[0053] S2. Take 40% of the total amount of purified water, add the tridecapeptide and natural polysaccharide mixture in proportion, and stir at room temperature at a stirring speed of 120 r / min for 30 minutes to obtain a premixed solution B;

[0054] S3. Slowly pour the premixed liquid B into the container containing the premixed liquid A, stir evenly, then add allantoin and hydroxyapatite, stir evenly, and obtain a skin repair liquid dressing with a uniform texture.

[0055] Example 3

[0056] A skin repair liquid dressing, comprising the following raw material components by weight percentage: 1% carbomer, 0.05% hyaluronic acid, 5% glycerol, 0.1% tridecapeptide, 0.1% allantoin, 0.2% nano-scale β-tricalcium phosphate, 0.8% natural polysaccharide mixture, and the balance purified water. The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, Panax notoginseng polysaccharide, and Polygonatum cyrtonema polysaccharide; the mass ratio of pomegranate flower polysaccharide, Panax notoginseng polysaccharide, and Polygonatum cyrtonema polysaccharide is 4:3:1; the average particle size of nano-scale β-tricalcium phosphate is 300nm.

[0057] The present invention also provides a method for preparing a skin repair liquid dressing, comprising the following steps:

[0058] S1. Take 80% of the total amount of purified water, slowly add carbomer, hyaluronic acid and glycerin in proportion, stir evenly, and let stand for 1 hour to allow it to fully soak and swell, to obtain premix A;

[0059] S2. Take 20% of the total amount of purified water, add the tridecapeptide and natural polysaccharide mixture in proportion, and stir at room temperature at a stirring speed of 150 r / min for 20 minutes to obtain a premixed solution B;

[0060] S3. Slowly pour the premixed liquid B into the container containing the premixed liquid A, stir evenly, then add allantoin and hydroxyapatite, stir evenly, and obtain a skin repair liquid dressing with a uniform texture.

[0061] Comparative Example 1

[0062] The difference between this comparative example and Example 2 is that the skin repair liquid dressing in this example does not contain nano-scale β-tricalcium phosphate; the preparation method of the skin repair liquid dressing in this example is basically the same as that of Example 2.

[0063] Comparative Example 2

[0064] The difference between this comparative example and Example 2 is that the skin repair liquid dressing in this example does not contain the natural polysaccharide mixture; the preparation method of the skin repair liquid dressing in this example is basically the same as that of Example 2.

[0065] Comparative Example 3

[0066] The difference between this comparative example and Example 2 is that the natural polysaccharide mixture in the skin repair liquid dressing of this example does not contain pomegranate flower polysaccharide, and the natural polysaccharide mixture is a mixture of Panax notoginseng polysaccharide and Polygonatum cyrtonema polysaccharide; the mass ratio of Panax notoginseng polysaccharide and Polygonatum cyrtonema polysaccharide is 2:1; the preparation method of the skin repair liquid dressing of this example is basically the same as that of Example 2.

[0067] Comparative Example 4

[0068] The difference between this comparative example and Example 2 is that the natural polysaccharide mixture in the skin repair liquid dressing of this example does not contain Panax notoginseng polysaccharide, and the natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide and Polygonatum cyrtonema polysaccharide; the mass ratio of pomegranate flower polysaccharide to Polygonatum cyrtonema polysaccharide is 3:1; the preparation method of the skin repair liquid dressing of this example is basically the same as that of Example 2.

[0069] Comparative Example 5

[0070] The difference between this comparative example and Example 2 is that the Polygonatum sibiricum polysaccharide is replaced by Pueraria lobata polysaccharide in the natural polysaccharide mixture in the skin repair liquid dressing of this example, and the natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, Panax notoginseng polysaccharide and Pueraria lobata polysaccharide; the mass ratio of pomegranate flower polysaccharide, Panax notoginseng polysaccharide and Pueraria lobata polysaccharide is 3:2:1; the preparation methods of Pueraria lobata polysaccharide and Polygonatum sibiricum polysaccharide are the same.

[0071] Comparative Example 6

[0072] The difference between this comparative example and Example 2 is that the average particle size of the nano-scale β-tricalcium phosphate in the skin repair liquid dressing of this example is 30 μm; the preparation method of the skin repair liquid dressing of this example is basically the same as that of Example 2.

[0073] Comparative Example 7

[0074] The difference between this comparative example and Example 2 is that in the preparation process of the Polygonatum cyrtonema polysaccharide in the skin repair liquid dressing of this example, the hot water extraction conditions are consistent with the hot water extraction conditions in the preparation process of pomegranate flower polysaccharide: temperature 100°C, water-to-solid ratio of 25:1 (mL / g), and extraction time is 160min.

[0075] Test Example 1 Safety Experiment

[0076] The skin repair liquid dressings of Examples 1-3 and Comparative Examples 1-7 were subjected to animal skin irritation tests, skin sensitization tests, and in vitro cytotoxicity tests, respectively, and the results showed that they were safe and non-toxic.

[0077] Test Example 2 Antibacterial Experiment

[0078] The antibacterial efficacy was determined according to the "Antibacterial Efficacy Test Method" of the 2020 edition of the Chinese Pharmacopoeia, and the number of surviving colonies in the test solution Sabouraud grape liquid culture medium for 24 hours was determined. The test bacteria were: Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, Propionibacterium acnes. The antibacterial rates of Examples 1 to 3 and Comparative Examples 1 to 7 are shown in Table 1 below.

[0079] Table 1 Antibacterial effect of skin repair liquid dressing

[0080]

[0081] It can be seen from the above table that the skin repair liquid dressing prepared by the present invention has an inhibition rate of greater than >99.9% on Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Candida albicans, Aspergillus niger, and Propionibacterium acnes; while in Comparative Example 1, nano-scale β-tricalcium phosphate was not added, and its antibacterial effect was slightly weakened, indicating that the addition of nano-scale β-tricalcium phosphate has a good barrier effect, can more effectively isolate bacteria, and provide a better closed environment for wound repair of skin tissue; Comparative Example 2 did not add the natural polysaccharide mixture, and its antibacterial effect was significantly weakened. The results of Comparative Examples 3 to 5 and Comparative Example 7 show that pomegranate flower polysaccharide, Panax japonicus polysaccharide, and Polygonatum cyrtonema polysaccharide have a synergistic effect, and the extraction method of the polysaccharide also has a certain influence on its antibacterial activity.

[0082] Test Example 3 Wound repair performance

[0083] 110 SPF SD rats were randomly divided into 11 groups, each with 10 rats, and the skin repair liquid dressings prepared in Examples 1-3 and Comparative Examples 1-7 and the commercially available recombinant collagen skin repair dressing (brand: Kefumei) were applied respectively. Each rat was anesthetized by intraperitoneal injection of 1.5 ml / kg 10% chloral hydrate, and then the dorsal hair was removed and the skin was disinfected. A 1.5 cm2 area was cut off from the back of each rat. 2A wound of full thickness. Then, the skin repair liquid dressings of Examples 1-3, Comparative Examples 1-7 and the commercially available recombinant collagen skin repair dressing were applied to the wound surface, once in the morning and once in the evening every day. The healing of the rat wounds and the scars after complete healing were observed on the 2nd, 4th and 6th days after spraying. Wound healing rate = (initial wound area - wound area measured on the nth day) / initial wound area × 100%; after complete healing, the wound surface is closed, no scars are seen, and the skin surface is smooth, which is judged to be well repaired, and the proportion of well-repaired rats is counted; the experimental results are shown in Table 2.

[0084] Table 2 Wound repair performance of skin repair liquid dressing

[0085]

[0086] The above results show that the skin repair liquid dressing prepared in Examples 1-3 of the present invention has a fast film-forming time, has a good repair effect on skin damage, has a fast skin healing speed, and has a high repair efficiency. Comparative Example 1 does not add nano-scale β-tricalcium phosphate (β-TCP), and its wound healing rate is significantly slowed down. Comparative Example 6 adds micron-scale β-tricalcium phosphate and the repair effect is also weakened to a certain extent, indicating that β-TCP can significantly promote skin wound repair, β-TCP has excellent biocompatibility, biological activity and the ability to promote new tissue formation, stimulate cell proliferation and migration, thereby accelerating wound healing, in addition, nano-scale particles have a larger specific surface area, which helps to release bioactive ions and further promote the repair process. β-TCP can also regulate the wound microenvironment, reduce inflammatory response, reduce the degree of fibrosis, and then reduce scar formation. These mechanisms work together to help better healing and reduce the generation of scars. Example 2 Compared with Comparative Examples 2-5 and Comparative Example 7, adding a certain ratio of the homemade pomegranate flower polysaccharide, Panax notoginseng polysaccharide, and Polygonatum multiflorum polysaccharide of the present invention can synergistically significantly improve the repair efficiency, shorten the healing time, and increase the proportion of good repairs. Natural polysaccharides have excellent moisturizing and adhesion properties, help to form a protective film, prevent wound drying and infection, and can also stimulate cell proliferation and migration, accelerate wound healing. In addition, natural polysaccharides usually have anti-inflammatory effects, can reduce inflammatory reactions, and thus scar formation.

[0087] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A skin repair liquid dressing, characterized in that: The invention comprises the following raw material components by weight percentage: 0.1% to 1% of carbomer, 0.05% to 0.25% of hyaluronic acid, 1% to 5% of glycerol, 0.05% to 0.1% of tridecapeptide, 0.1% to 0.2% of allantoin, 0.05% to 0.2% of nano-scale beta-tricalcium phosphate, 0.4% to 0.8% of natural polysaccharide mixture, and the balance of purified water.

2. A skin repair liquid dressing according to claim 1, characterized in that: The natural polysaccharide mixture is a mixture of pomegranate flower polysaccharide, Panax notoginseng polysaccharide and Polygonatum cyrtonema polysaccharide.

3. A skin repair liquid dressing according to claim 2, characterized in that: The mass ratio of the pomegranate flower polysaccharide, the panax japonicus polysaccharide and the polygonatum cyrtonema polysaccharide is: (2-4): (1-3):

1.

4. The skin repair liquid dressing according to claim 1, characterized in that: The preparation method of the pomegranate flower polysaccharide comprises the following steps: extracting the dried pomegranate flowers with hot water, filtering, concentrating the filtrate, adding ethanol to the filtrate, centrifuging after standing, separating the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying to obtain the pomegranate flower polysaccharide; the hot water extraction conditions include: controlling the temperature to 80-100°C, the water-to-material ratio to 20-30:1 (mL / g), and the extraction time to 2-3 hours.

5. The skin repair liquid dressing according to claim 1, characterized in that: The preparation method of the polysaccharide of Panax japonicus is as follows: extracting the dried Panax japonicus by enzymatic hydrolysis with cellulase, filtering, concentrating the filtrate, adding ethanol to the filtrate, standing it and then centrifuging it to separate the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying it to obtain the polysaccharide of Panax japonicus; The conditions for enzymolysis of the cellulase include: enzyme dosage of 0.04-0.06 g / g, enzymolysis temperature of 40-50° C., water-to-material ratio of 30-50:1 (mL / g), and enzymolysis time of 2-3 hours.

6. The skin repair liquid dressing according to claim 1, characterized in that: The preparation method of the polygonatum cyrtonema polysaccharide is as follows: extracting the dried polygonatum cyrtonema with hot water, filtering, concentrating the filtrate, adding ethanol to the filtrate, standing and centrifuging to separate the precipitate, deproteinizing and depigmenting the precipitate, dialyzing and freeze-drying to obtain the polygonatum cyrtonema polysaccharide; the hot water extraction conditions include: controlling the temperature to 65-80°C, the water-to-material ratio to 30-40:1 (mL / g), and the extraction time to 2-3 hours.

7. The skin repair liquid dressing according to claim 1, characterized in that: The average particle size of the nano-scale β-tricalcium phosphate is 100-600nm.

8. The method for preparing a skin repair liquid dressing according to any one of claims 1 to 7, characterized in that: The following steps are involved: S1. Take 60% to 80% of the total amount of purified water, slowly add carbomer, hyaluronic acid and glycerin in proportion, stir evenly, and let stand for 1 to 2 hours to allow it to fully soak and swell, to obtain premix A; S2. Take 20% to 40% of the total amount of purified water, add the tridecapeptide and natural polysaccharide mixture in proportion, and stir at room temperature at a stirring speed of 100 to 150 r / min for 20 to 30 minutes to obtain a premixed solution B; S3. Slowly pour the premixed liquid B into the container containing the premixed liquid A, stir evenly, then add allantoin and nano-scale β-tricalcium phosphate, stir evenly, and obtain a skin repair liquid dressing with uniform texture.

9. Use of the skin repair liquid dressing according to any one of claims 1 to 7 in the preparation of skin antibacterial products.

10. Use of the skin repair liquid dressing according to any one of claims 1 to 7 in the preparation of products for preventing pigmentation after skin wound healing, reducing scars and promoting wound healing.

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