Method for determining soil moisture necessary for germination of solanum rostratum seeds

Abstract

The invention provides a method for determining soil moisture necessary for germination of solanum rostratum seeds. The method comprises the specific steps of soil sample preparation, soil moisture content determination, different soil moisture content setting treatment, seed sowing and culture, observation and recording, and data analysis and threshold determination. The universality and applicability of the method are verified by selecting different types of representative soil in different regions, the research result is reliable, and a scientific basis can be provided for prevention and control of invasion of solanum rostratum.

Description

Technical Field

[0001] The invention relates to the technical field of prevention and control of alien invasive species, in particular to a method for determining necessary soil moisture for germination of Solanum serrata seeds. Background Art

[0002] Solanum serratum is an invasive alien plant that poses a serious threat to the ecological environment. The germination of its seeds is affected by many factors, among which soil moisture content and soil type are important factors. Accurately understanding the germination of Solanum serratum seeds under different soil moisture contents and soil types is crucial for formulating effective prevention and control measures.

[0003] At present, there are still deficiencies in the research on the relationship between Solanum serrata seed germination and soil moisture content and soil type. There is a lack of research on Solanum serrata seed germination based on representative soil types in different regions and different soil moisture contents. This makes it difficult to accurately grasp the key factors of Solanum serrata seed germination in actual ecological prevention and control work, and it is impossible to formulate targeted and efficient prevention and control strategies.

[0004] Therefore, in order to better prevent and control the invasion of Solanum spinulosa, it is necessary to conduct research on the effects of different soil types and moisture contents on the germination of Solanum spinulosa seeds, so as to determine the soil moisture threshold for seed germination and provide a scientific basis for the prevention and control of the alien invasive plant Solanum spinulosa. Summary of the invention

[0005] In view of the unclear soil type and soil moisture content that affect the germination of Solanum serratum seeds, the present invention provides a method for determining the soil moisture required for the germination of Solanum serratum seeds, comprising the following steps: S100: soil sample preparation; S200: soil moisture content determination; S300: Set different soil moisture content treatments; S400: Seed sowing and cultivation; S500: Observation and recording; S600: Data analysis and threshold determination.

[0006] The soil samples described in S100 refer to representative soil samples of different types collected from different regions.

[0007] The soil moisture content determination described in S200 refers to determining the air-dried soil moisture content and saturated moisture content of different soil samples.

[0008] The different soil moisture content treatments described in S300 are set according to the saturated moisture content of each soil sample. Distilled water is added to the air-dried soil, and a number of equal-difference moisture content gradients are set for each soil to form sample treatments with different humidity gradients. Each treatment is repeated multiple times.

[0009] The seed sowing and cultivation described in S400 refers to evenly sowing Solanum serrata seeds in culture dishes treated with different soils and covering them with lids, placing them in an artificial climate box with a suitable temperature, setting a light / dark photoperiod, and cultivating them continuously.

[0010] The observation and recording in S500 refers to taking the germination standard as the seed radicle slightly protruding from the seed coat, regularly observing and recording the seed germination every day, taking out the germinated seeds, and continuously observing and recording for a period of time until no more seeds germinate.

[0011] The data analysis and threshold determination in S600 refers to analyzing the effects of different soil treatments on seed germination rates and determining different soil moisture content thresholds that restrict seed germination.

[0012] The beneficial effects of the present invention are: 1. Selecting different types of soils that are representative of different regions in my country covers a wide range, making the research method more universal and applicable; 2. By setting up different soil moisture content gradient treatments, the germination rate of Solanum serratum seeds under different moisture conditions was systematically measured to determine the soil moisture threshold required for Solanum serratum seeds to germinate; 3. Each treatment was repeated multiple times to ensure the reliability of the soil moisture threshold for seed germination. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a flow chart of the present invention. DETAILED DESCRIPTION

[0014] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the present invention. It should be understood that the specific implementation methods described here are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0015] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0016] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0017] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments. Example

[0018] As a preferred embodiment of the present invention, the S100 soil sample preparation includes: S110: Soil selection Considering that different soil types may have different effects on the germination of Solanum serrata seeds, four representative red-yellow soils (obtained from the National Field Scientific Observation and Research Station of Huitong Forest Ecosystem in Hunan Province), aeolian sandy soil (obtained from the mobile sandy land in Zhangwu County, Fuxin City, Liaoning Province), meadow soil (obtained from the Wulan Aodu Desertification Control Ecological Experimental Station in Wengniute Banner, Chifeng City, Inner Mongolia Autonomous Region), and black soil (obtained from the National Field Scientific Observation and Research Station of Farmland Ecosystem in Hailun, Heilongjiang Province) were selected as experimental soils; S120: Soil treatment The soil lumps were broken up and then passed through a 1 mm sieve to remove larger particles and impurities.

[0019] As a preferred embodiment of the present invention, in the S200 soil moisture content determination, it includes: S210: Determination of moisture content of air-dried soil Use a scoop to mix the air-dried soil sample carefully, scoop out about 5 grams, spread it evenly in an aluminum pan, cover it and weigh it to an accuracy of 0.001 grams. Open the lid of the aluminum box and bake it in an oven preheated to 105±2℃ for 6 hours. Take it out and quickly cover it, move it into a desiccator to cool it to room temperature (about 20 minutes), and weigh it immediately. Repeat the measurement of the moisture content of each air-dried soil sample 5 times to reduce the error; The moisture content of different soil samples was calculated using the formula: soil moisture content (weight %) = (original soil weight - drying soil weight) / drying soil weight × 100% = water weight / drying soil weight × 100%. The results of the experiment showed that the air-dried soil moisture content of red-yellow soil was 1.6%; the air-dried soil moisture content of sandy soil was 0.26%; the air-dried soil moisture content of meadow soil was 0.7%; the air-dried soil moisture content of black soil was 2.98%; S220: Determination of soil saturated water content For each soil type, the ring knife method was used to obtain soil samples. Filter paper was added to the bottom of the ring knife, and then it was soaked in a container of water for 6 hours to ensure that the soil was fully soaked. After that, the soil soaked with water was placed in an aluminum box and weighed and recorded. Next, the aluminum box was placed in an oven and dried at 105°C for 24 hours. After cooling, it was weighed again and the saturated water content was calculated according to the formula. The test results showed that the saturated water content of red-yellow soil was 41.8%; the saturated water content of sandy soil was 18.8%; the saturated water content of meadow soil was 26%; and the saturated water content of black soil was 46.2%.

[0020] As a preferred solution of the present invention, in S300, different soil moisture content processing is set, including: S310: According to the maximum water holding capacity (i.e. saturated water content) of each soil sample, five sample water content treatments are set as follows: ① maximum water holding capacity (FMC); ② 4 / 5 FMC; ③ 3 / 5 FMC; ④ 2 / 5 FMC; ⑤ 1 / 5 FMC; S320: Weigh an equal amount of soil samples, add different masses of distilled water to each set soil moisture content gradient, mix carefully to form samples with different moisture content gradients, and set 5 repetitions for each treatment.

[0021] As a preferred embodiment of the present invention, in the S400 seed sowing and cultivation, it includes: S410: Prepare experimental materials Prepare 100 plastic culture dishes, 3,000 mature Solanum serrata seeds and a constant temperature incubator; S420: Seeding 30 seeds of Solanum serrata were randomly selected and evenly sown in the soil of plastic culture dishes with different treatments to ensure that the seeds were fully in contact with the soil and provide good initial conditions for seed germination; S430: Cultivation The culture dishes were weighed and recorded after sowing, so as to monitor the moisture changes later and add water appropriately. The culture dishes were covered with lids to prevent moisture evaporation. They were then placed in an artificial climate box at 25°C with a photoperiod of 12 hours light / 12 hours dark.

[0022] As a preferred solution of the present invention, in S500 observation and recording, it includes: S510: The germination standard is that the radicle protrudes 2 mm from the seed coat. Seed germination is observed and recorded once every 24 hours. The number of germinated seeds is recorded. After recording, the germinated seeds are removed to avoid confusion. S520: Observe the seed germination for 14 consecutive days until there is no continuous germination.

[0023] As a preferred solution of the present invention, in S600 data analysis and threshold determination, it includes: S610: Analyze and record data, calculate seed germination rates under different types of soil and water content, conduct in-depth analysis of the soil moisture threshold that affects seed germination, and clarify the effects of different moisture content and soil types on seed germination.

[0024] This method can more accurately determine the soil moisture threshold required for Solanum spinulosa seed germination, providing a more accurate theoretical basis for the prevention and control of Solanum spinulosa.

[0025] The above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention.

Claims

1. A method for determining the necessary soil moisture for Solanum serrata seed germination, characterized in that: The following steps are involved: S100: soil sample preparation; S200: soil moisture content determination; S300: Set different soil moisture content treatments; S400: Seed sowing and cultivation; S500: Observation and recording; S600: Data analysis and threshold determination.

2. A method for determining the necessary soil moisture for Solanum serrata seed germination according to claim 1, characterized in that: The soil samples described in S100 refer to representative soil samples of different types collected from different regions.

3. A method for determining the necessary soil moisture for Solanum serrata seed germination according to claim 1, characterized in that: The soil moisture content determination described in S200 refers to determining the air-dried soil moisture content and saturated moisture content of different soil samples.

4. The method for determining the necessary soil moisture for Solanum serrata seed germination according to claim 1, characterized in that: The different soil moisture content treatments described in S300 are set according to the saturated moisture content of each soil sample. Distilled water is added to the air-dried soil, and a number of equal-difference moisture content gradients are set for each soil to form sample treatments with different humidity gradients. Each treatment is repeated multiple times.

5. The method for determining the necessary soil moisture for germination of Solanum serrata seeds according to claim 1, characterized in that: The seed sowing and cultivation described in S400 refers to evenly sowing Solanum serrata seeds in culture dishes treated with different soils and covering them with lids, placing them in an artificial climate box with a suitable temperature, setting a light / dark photoperiod, and cultivating them continuously.

6. The method for determining the necessary soil moisture for Solanum serrata seed germination according to claim 1, characterized in that: The observation and recording in S500 refers to taking the germination standard as the seed radicle slightly protruding from the seed coat, regularly observing and recording the seed germination every day, taking out the germinated seeds, and continuously observing and recording for a period of time until no more seeds germinate.

7. The method for determining the necessary soil moisture for Solanum serrata seed germination according to claim 1, characterized in that: The data analysis and threshold determination in S600 refers to analyzing the effects of different soil treatments on seed germination rates and determining different soil moisture content thresholds that restrict seed germination.

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