Acaricidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticides, and relates to an acaricidal composition and application thereof, the acaricidal composition comprises an active component A and an active component B, the active ingredient A is a compound shown in a formula (I), and the active ingredient B is any one of lufenuron and fluazinam. The acaricidal composition can be used for efficiently preventing and treating phytophagous harmful mites, obviously reducing harmful mite populations, improving the quick-acting property and delaying the development of drug resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to a mite-killing composition and application thereof. Background Art

[0002] The two-spotted spider mite (Tetranychus urticae Koch) belongs to the genus Tetranychus of the family Tetranychidae. It is an important omnivorous pest mite with a wide range of host plants. Currently, more than 140 families and 1,100 species of hosts have been discovered. The two-spotted spider mite is rampant from mid-June to mid-August. It often harms vegetables, flowers and fruit trees with juveniles, nymphs and adults, causing the leaves to lose green and scorch and fall off, seriously affecting photosynthesis, inhibiting the growth of vegetables and flowers and fruit development, and ultimately affecting crop yield and quality. In recent years, the two-spotted spider mite has changed from a minor pest mite to a major pest mite, and has now become one of the important pest mites for crops such as vegetables, flowers and fruit trees.

[0003] Lufenuron is a chitin synthesis inhibitor. The agent kills pests by acting on insect larvae and preventing the molting process. It has stomach poison and contact killing, multiple egg-killing and high-efficiency insecticidal effects. It has little effect on the natural enemies of pests and is very safe for humans and animals.

[0004] Fluazinam belongs to the dinitroaniline fungicide. It is a broad-spectrum and highly effective protective fungicide. It is highly effective against fungi such as Alternaria, Immunomyces, Monoparana, and Venturia. In addition, it also has the function of controlling phytophagous mites.

[0005] Due to the irrational use of pesticides and the characteristics of two-spotted spider mites with fast reproduction speed and serious overlapping generations, two-spotted spider mites have developed serious resistance to a variety of insecticides such as lufenuron and fluazinam. Therefore, in order to reduce the resistance of two-spotted spider mites to lufenuron and fluazinam as much as possible and improve the control effect, the present application compounded the compound of formula (I) with lufenuron and fluazinam, which can significantly enhance the effect within a certain mixing range, reduce costs, reduce the amount of pesticides, prolong the duration of effect and delay the development of resistance. Summary of the invention

[0006] In order to solve the above problems existing in the prior art, the present invention provides a miticide composition, which can effectively control herbivorous mites, show a synergistic effect within a certain proportion range, reduce the cost of use, reduce the amount of medicine used, prolong the lasting effect and delay the development of drug resistance.

[0007] In order to solve the above technical problems, the present invention provides the following technical solution: an acaricidal composition, the acaricidal composition comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound of formula (I):

[0008] The active ingredient B is any one of lufenuron and fluazifop;

[0009] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:1;

[0010] Furthermore, the mass ratio of the compound of formula (I) to lufenuron is 1:24 to 24:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to lufenuron is 1:24, 1:18, 1:14, 1:7, 5:2, 7:1, 15:1, 20:1, 24:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to fluazinam is 1:20 to 24:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to fluazinam is 1:20, 1:15, 1:10, 1:5, 3:2, 5:1, 12:1, 24:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to lufenuron is 1:24 to 7:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to fluazinam is 1:15 to 5:1;

[0016] Furthermore, based on the total weight of the acaricidal composition being 100 wt%, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 1 to 95 wt%;

[0017] Furthermore, the sum of the content of the active ingredient A and the active ingredient B in the acaricidal composition is 5 to 80 wt %;

[0018] Furthermore, the acaricide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers;

[0019] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, alpha olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0020] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acid, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0021] The emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0022] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0023] Disintegrants: The disintegrants are selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0024] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0025] The defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0026] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0027] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0028] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0029] The synergist is selected from synergist phosphite, synergist ether; and / or

[0030] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0031] Furthermore, the acaricide composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation;

[0032] The solid preparations include powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0033] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions or microcapsule suspension-suspoemulsions;

[0034] Further, the powder is a free-flowing powder preparation containing active ingredients suitable for dusting or spreading;

[0035] Furthermore, the granules are granular preparations containing active ingredients that can flow freely within a certain particle size range;

[0036] Furthermore, the spherical preparation is a spherical preparation (generally with a diameter greater than 6 mm) containing an active ingredient;

[0037] Furthermore, the tablet is a sheet-like preparation containing an active ingredient with a certain shape and size (usually having two flat or convex surfaces, with the distance between the two surfaces being less than the diameter);

[0038] Further, the strip is a strip or rod-shaped preparation containing an active ingredient (generally a few centimeters in length and a few millimeters in width / diameter, i.e., the length is greater than the diameter / width);

[0039] Furthermore, the wettable powder is a powdered preparation in which the active ingredient is dispersed in water into a suspension;

[0040] Furthermore, the oil dispersible powder is a powdered preparation in which the active ingredient is dispersed into a suspension in an organic solvent;

[0041] Furthermore, the emulsion powder is a powdered preparation in which the active ingredient is dissolved in an organic solvent, encapsulated in a soluble or insoluble inert ingredient, and dispersed in water to form an oil-in-water emulsion;

[0042] Further, the water dispersible granules are granular preparations that disintegrate in water and disperse the active ingredients into a suspension;

[0043] Furthermore, the emulsifiable granules are granular preparations in which the active ingredients are dissolved in an organic solvent, encapsulated in a soluble or insoluble inert ingredient, and dispersed in water to form an oil-in-water emulsion;

[0044] Furthermore, the water-dispersible tablet is a sheet-like preparation that disintegrates in water and disperses the active ingredients into a suspension;

[0045] Furthermore, the soluble powder is a powdered preparation in which the active ingredient forms a true solution in water and may contain inert ingredients that are insoluble in water;

[0046] Furthermore, the soluble granules are granular preparations in which the active ingredients form a true solution in water and may contain inert ingredients that are insoluble in water;

[0047] Furthermore, the soluble tablet is a sheet-like preparation in which the active ingredient forms a true solution in water and may contain an inert ingredient that is insoluble in water;

[0048] Furthermore, the soluble agent is a transparent or translucent liquid preparation containing active ingredients that is diluted with water and may contain inert ingredients that are insoluble in water;

[0049] Furthermore, the soluble gel is a gel preparation containing active ingredients that is diluted with water to form a true solution;

[0050] Furthermore, the oil preparation is a liquid preparation diluted with an organic solvent (or not diluted) to form a homogeneous phase and containing an active ingredient;

[0051] Furthermore, the film-spreading oil is an oil containing active ingredients that automatically spreads on the water surface to form an oil film;

[0052] Furthermore, the emulsifiable concentrate is a homogeneous liquid preparation containing active ingredients that is diluted and dispersed into an emulsion with water;

[0053] Furthermore, the latex is a latex preparation containing active ingredients that is diluted and dispersed into an emulsion with water;

[0054] Furthermore, the dispersible liquid preparation is diluted with water and dispersed into a homogeneous liquid preparation containing the active ingredient in suspension;

[0055] Furthermore, the ointment is a water-based ointment containing active ingredients and capable of forming a film, and is generally used directly;

[0056] Furthermore, the aqueous emulsion is an emulsion liquid preparation formed by the active ingredient (or its organic solution) in water;

[0057] Furthermore, the oil emulsion is an emulsion liquid preparation in which the active ingredient (or its aqueous solution) is in oil;

[0058] Furthermore, the microemulsion is a transparent or translucent microemulsion liquid preparation in which the active ingredient is dissolved in water and is used directly or after being diluted with water;

[0059] Furthermore, the fat agent is an oil or fat-based viscous preparation containing active ingredients, which is generally used directly;

[0060] Furthermore, the suspension concentrate is a stable suspension liquid preparation in which the active ingredient is dispersed in water as solid particles, and is generally diluted with water for use;

[0061] Furthermore, the microcapsule suspension is a stable suspension liquid preparation formed by microcapsules containing active ingredients dispersed in a liquid;

[0062] Furthermore, the oil suspension is a stable suspension liquid preparation in which the active ingredient is dispersed in a liquid as solid particles, and is generally diluted with an organic solvent for use;

[0063] Furthermore, the dispersible oil suspension is a stable suspension liquid preparation in which the active ingredient is dispersed in a non-aqueous medium as solid particles, and is generally diluted with water for use;

[0064] Furthermore, the suspoemulsion is a heterogeneous liquid preparation in which the active ingredient is stably dispersed in a continuous aqueous phase in the form of solid particles and water-insoluble tiny droplets;

[0065] Furthermore, the microcapsule suspension-suspension concentrate is a stable suspension liquid preparation in which the active ingredients are dispersed in water in the form of microcapsules and solid particles;

[0066] Furthermore, the microcapsule suspension-water emulsion is a heterogeneous liquid preparation in which the active ingredients are stably dispersed in the form of microcapsules and tiny droplets in a continuous aqueous phase;

[0067] Furthermore, the microcapsule suspension-suspoemulsion is a heterogeneous liquid preparation in which the active ingredients are stably dispersed in the form of microcapsules, solid particles and tiny droplets in a continuous aqueous phase;

[0068] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from soluble solutions, emulsifiable concentrates, aqueous emulsions, suspensions, suspoemulsions, microemulsions, and dispersible oil suspensions;

[0069] Furthermore, the solid preparation is selected from wettable powders and water dispersible granules; the liquid preparation is selected from emulsifiable concentrates, aqueous emulsions, and suspensions;

[0070] Furthermore, the wettable powder preparation method is to pre-crush the active ingredient and other adjuvants and mix them uniformly, and then grind them by a jet mill to a fineness that satisfies the requirement that at least 98wt% passes through a 45μm test sieve to obtain a wettable powder product;

[0071] Furthermore, the water dispersible granule preparation method is to add the active ingredient to the carrier according to the formula ratio, add the surfactant and other functional additives thereto, mix, add 10-25% water after air flow pulverization, and then knead, granulate, dry and sieve to obtain the water dispersible granule product;

[0072] Furthermore, the emulsifiable concentrate preparation method is to add the active ingredients into a carrier according to the formula ratio of the embodiment, add a surfactant and other functional additives thereto, and stir and mix them evenly in a stirring mixing kettle to obtain an emulsifiable concentrate product;

[0073] Furthermore, the preparation method of the aqueous emulsion is as follows: according to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase, and deionized water and antifreeze are mixed together to form a uniform water phase; the water phase is added to the oil phase, and after stirring evenly, a defoaming agent is added and subjected to high-speed shearing to form a well-dispersed aqueous emulsion product;

[0074] Furthermore, the suspension preparation method is to place the active ingredient, surfactant and other functional additives in a reaction kettle in order according to the formula ratio, add water to mix evenly, and then perform high-speed shearing, wet sand grinding, and finally homogenization filtration to obtain the suspension product;

[0075] The present invention also discloses the use of the above-mentioned pesticide composition in preventing and controlling harmful mites;

[0076] Furthermore, the harmful mites are herbivorous harmful mites;

[0077] Furthermore, the phytophagous pest mites are: Panonychus citri McGregor, Eotetranychus kankitus (Ehara), Phyllocoptes oleivora, Tetranychus cinnabarinus (Boisduval), Tetranychus urticae Koch;

[0078] Furthermore, the pesticide composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled or to the medium in which they grow.

[0079] Beneficial effects of the present invention:

[0080] (1) The acaricidal composition of the present invention has a significant synergistic effect at a certain ratio, especially having a good control effect on two-spotted spider mites;

[0081] (2) It can significantly reduce the population of phytophagous mites, improve the rapidity of efficacy and delay the development of pesticide resistance;

[0082] (3) It is safe and efficient, safe for crops, non-target organisms, beneficial organisms and natural enemies, and has the effect of increasing production and ensuring income. DETAILED DESCRIPTION

[0083] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0084] Preparation Example:

[0085] Preparation Example 1: 14% compound of formula (I)·lufenuron suspension (10:4)

[0086] Preparation formula: 10% compound of formula (I), 4% lufenuron, 2% calcium dodecylbenzene sulfonate, 3% fatty amine polyoxyethylene ether, 2% polycarboxylate, 5% sodium naphthalene sulfonate, 0.5% sodium p-hydroxybenzoate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4% ethylene glycol, 0.25% dimethyl silicone oil, and deionized water to make up the balance;

[0087] Preparation method: according to the formula ratio of the embodiment, the active ingredients, surfactants and other functional additives are placed in a reaction kettle in sequence, deionized water is added to mix evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0088] Preparation Example 2: 20% compound of formula (I)·fluazinam suspension (12:8)

[0089] Preparation formula: 12% compound of formula (I), 8% fluazinam, 4% calcium dodecylbenzene sulfonate, 1.5% polyether, 4% sodium lignin sulfonate, 0.2% sodium sorbate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 4.5% propylene glycol, 0.25% dimethyl silicone oil, and deionized water to make up the balance;

[0090] Preparation method: Same as Preparation Example 1.

[0091] Preparation Example 3: 4% compound of formula (I)·lufenuron emulsifiable concentrate (2.5:1.5)

[0092] Preparation formula: 2.5% compound of formula (I), 1.5% lufenuron, 15% propylene carbonate, 4.5% sodium lauryl sulfate, 12% fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0093] Preparation method: Add the active ingredients into the solvent according to the formula ratio of the embodiment, add the surfactant and other functional additives thereto, stir and mix them evenly in a stirring mixing kettle to obtain the emulsifiable concentrate product.

[0094] Preparation Example 4: 7.5% compound of formula (I)·lufenuron aqueous emulsion (4.5:3)

[0095] Preparation formula: 4.5% compound of formula (I), 3% lufenuron, 5% xylene, 12% cyclohexanone, 4% sodium dodecylbenzene sulfonate, 6.5% ethylene oxide-propylene oxide copolymer, 4% ethylene glycol, 0.5% glycerol, 0.2% silicone defoamer, 0.1% xanthan gum, 0.2% benzoic acid, and deionized water to make up the balance;

[0096] Preparation method: according to the formula ratio of the embodiment, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase, and deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shearing, the water phase is added to the oil phase to form a well-dispersed water emulsion product.

[0097] Preparation Example 5: 36% compound of formula (I)·fluazinam wettable powder (18:18)

[0098] Preparation formula: 18% compound of formula (I), 18% fluazinam, 15% sodium lignosulfonate, 6% castor oil polyoxyethylene ether phosphate, 5% naphthalene sulfonate, 6% bentonite, 4.5% white carbon black, and kaolin makes up the balance;

[0099] Preparation method: according to the formula ratio of the embodiment, the active ingredient is added into the carrier, and the surfactant and other functional additives are added thereto, mixed, and then air flow pulverized and mixed again to obtain the wettable powder product.

[0100] Preparation Example 6: 40% compound of formula (I)·lufenuron wettable powder (16:24)

[0101] Preparation formula: 16% compound of formula (I), 24% fluazinam, 10% sodium lignosulfonate, 2% castor oil polyoxyethylene ether phosphate, 5% naphthalene sulfonate, 10% starch, 5% white carbon black, and kaolin to make up the balance;

[0102] Preparation method: Same as Preparation Example 5.

[0103] Indoor toxicity example 1

[0104] Examples Reference is made to the Guidelines for Indoor Bioassay Tests for Pesticides Part 7: Determination of Combined Effects of Mixtures NY / T1154.7-2006.

[0105] Test insect source: Two-spotted spider mites (raised in the R&D center) were selected. Female adult mites were raised indoors and in the same physiological state. The temperature was (25±1)℃. The relative humidity was 65%±5%, and the photoperiod was 16 / 8h (L / D).

[0106] Preparation of the drug: Use acetone to dissolve the original drug, and then dilute it with 0.1% Tween-80 aqueous solution. Prepare the mother liquor of each single dose separately, and design the proportion of each group according to the purpose of mixing and the activity of the drug. Each single dose and each group of mixed drugs are prepared in 5 series of mass concentrations according to the equal ratio method.

[0107] The test method for determining the sublethal concentration of each treatment on adult mites is as follows: Select female adult mites with the same growth, stick the back of the mites on a glass slide with double-sided tape, and examine them under a microscope after 1 hour, and keep 20 individuals with good survival status. Then immerse the glass slide in each concentration of the drug for 5 seconds and then take it out, absorb the excess liquid with filter paper, and place it in a light constant temperature incubator with a temperature of (25±1)℃, a relative humidity of 65%±5%, and a light cycle of 16 / 8h (L / D). After 36 hours of drug treatment, count the number of deaths of adult mites.

[0108] Each treatment was repeated at least 4 times, and a treatment without drug (including all organic solvents and emulsifiers) was set as a blank control.

[0109] Data statistics and analysis: The total number of mites and the number of dead mites were recorded separately (death standard: use a needle tip to poke the mite's appendages, those that do not move are dead, and those that move are alive).

[0110] Determination of sublethal effects of each treatment on adult mites Test method: Take a number of fresh cowpea leaves, put them with the back side facing up in a petri dish containing a sponge, and inoculate 200 female adult mites that have just emerged within 24 hours on each leaf, let them rest for 30 minutes, and then use the LC of each treatment after the adult mites are stable. 10 Spray treatment with the dosage.

[0111] Use a spray tower for quantitative spraying, take 2 mL of the solution to be used, spray for 60 seconds, and continue to raise the fish in the light incubator after the solution has dried.

[0112] After 24 hours, healthy and strong adult mites were picked and placed in a 2 cm diameter fresh cowpea leaf dish without pesticides for single feeding. The number of insects observed in each treatment was not less than 100, and the egg laying amount of adult mites was recorded once every 24 hours until all adult mites died. Clean water was used as the control.

[0113] Data Analysis:

[0114] According to the survey data, the adjusted mortality rate of each treatment was calculated using the following formula, with the results retained to two decimal places:

[0115]

[0116] Where:

[0117] P——mortality rate, in percentage (%);

[0118] K——indicates the number of dead insects, in units of heads;

[0119] N——represents the total number of insects treated, in heads.

[0120]

[0121] Where:

[0122] P1——adjusted mortality rate, in percentage (%);

[0123] P t ——Treatment mortality rate, expressed as percentage (%);

[0124] P0——blank control mortality rate, in percentage (%).

[0125] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0126] The data were processed using the probability value analysis method. The DPS statistical analysis system can be used to analyze and find the LC of the toxicity regression line. 10 Value, LC 50 The activity of the test agent on the biological test material was evaluated by using the b value and its 95% confidence limit.

[0127] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0128]

[0129] Where:

[0130] ATI——Actual Toxicity Index of Mixture;

[0131] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0132] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0133] TTI=TI A *P A +TI B *P B

[0134] Where:

[0135] TTI – Theoretical Toxicity Index of Mixtures;

[0136] TI A ——A: toxicity index of the agent;

[0137] P A ——The percentage of agent A in the mixture, in percentage (%);

[0138] TI B ——Toxicity index of agent B;

[0139] P B ——The percentage of agent B in the mixture, in percentage (%).

[0140]

[0141] Where:

[0142] CTC – Co-toxicity coefficient;

[0143] ATI——Actual Toxicity Index of Mixture;

[0144] TTI - Theoretical Toxicity Index of Mixture.

[0145] The co-toxicity coefficient of the combination CTC≥120 shows a synergistic effect; CTC≤80 shows an antagonistic effect; 80<CTC<120 shows an additive effect.

[0146] Test results:

[0147] The test results in Table 1 and Table 2 show that lufenuron and fluazinam show high toxicity to two-spotted spider mites. 50 The concentrations of the compound of formula (I) and lufenuron were 10.541 mg / L and 9.139 mg / L respectively. The compound of formula (I) and fluazinam showed a synergistic effect in the range of 1:24 to 24:1; the compound of formula (I) and fluazinam showed a synergistic effect in the range of 1:20 to 24:1.

[0148] Table 1 Combined toxicity test of different ratios of the compound of formula (I) and lufenuron against Tetranychus urticae

[0149]

[0150] Table 2 Combined toxicity test of different ratios of the compound of formula (I) and fluazinam against Tetranychus urticae

[0151]

[0152]

[0153] Sublethal doses of the compound of formula (I) and lufenuron and fluazinam in different ratios 10The effects of treating adult spider mites on their lifespan and egg hatching rate are shown in Table 3. It can be seen that the lifespan and egg laying rate of contemporary adult mites are reduced to varying degrees. Among them, the sublethal dose LC when the mass ratio of the compound of formula (I) to lufenuron is 5:2 10 After treating adult spider mites, compared with the blank control and a single dose, the life span, egg laying and egg hatching rate of the current generation of adult mites were most significantly reduced; the sublethal dose LC when the mass ratio of the compound of formula (Ⅰ) to fluazinam was 3:2 10 After treating adult two-spotted spider mites, the lifespan, egg laying amount and egg hatching rate of the current generation of adult mites were most significantly reduced compared with the blank control and a single dose.

[0154] Table 3 Effects of different combinations of the compound of formula (I) with lufenuron and fluazinam on the life span, egg laying and egg hatching rate of adult spider mites treated with sublethal doses

[0155]

[0156] Note: The above data are the average of the individual data of two-spotted spider mites observed in each treatment and the integer is retained.

[0157] Field test example 1 Field control test of two-spotted spider mite on greenhouse beans

[0158] Tested crops: kidney bean, variety: kidney bean;

[0159] Test subjects: Two-spotted spider mite;

[0160] Test location: Greenhouse in Pingdu, Qingdao, Shandong Province. The management level is good and the growth is uniform. No pesticides were used from emergence to application. The application period was the early flowering period, and the occurrence of two-spotted spider mites was severe.

[0161] Test reagents:

[0162] Table 4 Experimental treatment and dosage

[0163] Experimental treatment Experimental Drugs Dilution multiple 1 14% Formula (Ⅰ) compound·lufenuron suspension (10:4) 4000 2 4% Formula (Ⅰ) compound·lufenuron emulsifiable concentrate (2.5:1.5) 4000 3 20% Formula (I) compound·Fluazinam suspension (12:8) 4000 4 36% Formula (Ⅰ) compound·Fluazinam wettable powder (18:18) 4000 5 10% Lufenuron Suspension 2000 6 40% Fluazifop suspension 2000 7 Clean water treatment (CK) /

[0164] Experimental design: The experiment set up 6 pesticide treatments and 1 clean water treatment (CK), which were randomly arranged and repeated 3 times for each treatment. There were 15 beans in each treatment. The whole bean plant was sprayed with an electric sprayer, and both the front and back of the leaves were sprayed. The water consumption per mu was 45L.

[0165] Number and time of surveys: Investigate the basic number of two-spotted spider mites on each bean plant before applying the pesticide, and investigate the number of residual mites on the plants 1d, 3d, 14d, and 28d after applying the pesticide, and calculate the mite population reduction rate and control effect.

[0166] Data statistics and analysis: Duncan's new multiple range test was used to analyze the significance of differences.

[0167] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0168]

[0169]

[0170] Effects of different treatments on the control of Tetranychus urticae

[0171] Test results:

[0172] The control effects of each treatment on two-spotted spider mites are shown in Tables 5 and 6. The four mixed preparations all showed high rapid and long-lasting effects on two-spotted spider mites, and the control effects were all maintained above 80%. Among them, the control effect of 20% formula (Ⅰ) compound·fluazinam suspension (12:8) reached 93.01% 3 days after application, which was significant.

[0173] Table 5 Control effect of different treatments on bean two-spotted spider mite (fast effect)

[0174]

[0175] Note: The two-spotted spider mite (head) and control effect (%) in the above table are the average values ​​of each replicate. Lowercase letters represent significant differences at the 5% level.

[0176] Table 6 Control effect of different treatments on bean two-spotted spider mite (persistent effect)

[0177]

[0178] Note: The two-spotted spider mite (head) and control effect (%) in the above table are the average values ​​of each replicate. Lowercase letters represent significant differences at the 5% level.

[0179] In addition, no compound pesticides were found to cause phytotoxicity to crops in the test. The acaricidal composition or preparation can reduce production costs and use costs and is safe for crops. The above test shows that each mixed treatment has a good control effect on two-spotted spider mites, with good rapidity and persistence. During the test, it was found that each mixed treatment had no toxicity to natural enemies, and the host plants did not show symptoms of phytotoxicity such as leaf curling and leaf burning during the application process.

[0180] Field test example 2 Field control test of two-spotted spider mite on eggplant

[0181] Test crops: Eggplant, variety: Dalong;

[0182] Test subjects: Two-spotted spider mite;

[0183] Test location: Vegetable planting base in Qingzhou City, Weifang City, Shandong Province, with good management level and uniform growth. No pesticides were used from emergence to application. The application period was the fruit setting period, and the occurrence of two-spotted spider mites was severe.

[0184] Test reagents:

[0185] Table 7 Experimental treatment and dosage

[0186]

[0187]

[0188] Experimental design: The experiment set up 6 pesticide treatments and 1 clean water treatment (CK), which were randomly arranged and repeated 3 times for each treatment. There were 10 eggplant plants in each treatment. The whole eggplant plant was sprayed with a sprayer, and both the front and back of the leaves were sprayed. The water consumption per mu was 45L.

[0189] Survey times and times: Before applying the pesticide, investigate the base number of two-spotted spider mites on 4 mature leaves of each eggplant plant. Investigate the number of residual mites on the plants 1d and 7d after applying the pesticide, and calculate the mite population reduction rate and control effect.

[0190] Data statistics and analysis: Duncan's new multiple range test was used to analyze the significance of differences.

[0191] Calculation method of drug efficacy: The drug efficacy is calculated according to the following formula:

[0192]

[0193]

[0194] Effects of different treatments on the control of Tetranychus urticae on eggplant

[0195] Test results:

[0196] The control effects of each treatment on eggplant two-spotted spider mite are shown in Table 8. 14% formula (Ⅰ) compound·lufenuron suspension concentrate (10:4), 4% formula (Ⅰ) compound·lufenuron emulsifiable concentrate (2.5:1.5), 20% formula (Ⅰ) compound·fluazind suspension concentrate (12:8), and 36% formula (Ⅰ) compound·fluazind wettable powder (18:18) all showed high control effects on eggplant two-spotted spider mite. One day after application, the control effects were 86.33%, 84.15%, 87.38%, and 81.04%, respectively; 7 days after application, the control effects were 89.02%, 85.36%, 86.44%, and 83.73%, respectively; compared with the control, they all showed better rapidity and persistence.

[0197] Table 8 Control effect of different treatments on eggplant two-spotted spider mite

[0198]

[0199] Note: The two-spotted spider mite (head) and control effect (%) in the above table are the average values ​​of each replicate. Lowercase letters represent significant differences at the 5% level.

[0200] During the test, it was found that each mixed treatment had no toxicity to natural enemies and was safe to eggplant during application, with no symptoms of phytotoxicity such as leaf curling or leaf burning.

[0201] Although the present invention has been described in detail above with general instructions and specific implementation schemes, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, characterized in that: The acaricidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is a compound of formula (I): The active ingredient B is any one of lufenuron and fluazifop.

2. The acaricidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:30 to 30:

1.

3. The acaricidal composition according to claim 1, characterized in that: The mass ratio of the compound of formula (I) to lufenuron is 1:24 to 24:1; the mass ratio of the compound of formula (I) to fluazinam is 1:20 to 24:1; Preferably, the mass ratio of the compound of formula (I) to lufenuron is 1:24 to 7:1; the mass ratio of the compound of formula (I) to fluazinam is 1:15 to 5:

1.

4. The acaricidal composition according to claim 1, characterized in that: Based on the total weight of the acaricidal composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the acaricidal composition is 5 to 80 wt %.

5. The acaricidal composition according to claim 1, characterized in that: The acaricide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

6. The acaricidal composition according to claim 5, characterized in that: The acaricidal composition can be prepared into a formulation form permitted in agriculture, and the formulation form is selected from a solid formulation and / or a liquid formulation.

7. The acaricidal composition according to claim 6, characterized in that: The solid preparation is selected from wettable powders and water-dispersible granules; the liquid preparation is selected from soluble solutions, emulsifiable concentrates, aqueous emulsions, suspensions, suspoemulsions, microemulsions, and dispersible oil suspensions.

8. Use of the acaricidal composition according to any one of claims 1 to 7 for controlling harmful mites.

9. The use according to claim 8, characterized in that: The harmful mites are herbivorous harmful mites; Preferably, the phytophagous pest mites are Panonychus citri, Tetranychus citri, Tetranychus cinnabarinus, and Tetranychus urticae.

10. The use according to claim 9, characterized in that: The acaricidal composition and / or its preparation is applied at an effective dose to the harmful mites to be controlled or the medium where they grow.

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