Plant regulating agent for inhibiting thin and acerbity of litchi pulp as well as preparation method and application of plant regulating agent

Abstract

The invention provides a plant regulator for inhibiting thin and acerbity of litchi pulp as well as a preparation method and application thereof, and the plant regulator is prepared from the following raw materials in parts by weight: 30-35 parts of 6-benayl aminopurine, 8-10 parts of 6-furfurylaminopurine, 5-8 parts of gibberellic acid A4, 3-5 parts of an active agent, 3-5 parts of a dispersing agent, 2-3 parts of amino-oligosaccharin, 1-3 parts of an emulsifying agent and 1-1.5 parts of 28 homobrassinolide. The active agent is one of sodium dodecyl benzene sulfonate, sodium butyl naphthalene sulfonate, dodecylphenol polyoxyethylene ether and sodium aminobenzene sulfonate. The dispersing agent is one of sulfated castor oil, sodium lignin sulfonate and oleoyl polyamino carboxylate. And the emulsifier is one of polyethylene glycol 600 monooleate and Tween 80. The plant regulating agent is sprayed in the flower withering period of litchi trees, and compared with the method without spraying the plant regulating agent, after the plant regulating agent is sprayed, the litchi pulp thickness, the fructose content, the total sugar ratio and the solid acid ratio are remarkably increased, and the quality of litchi fruits is effectively improved.

Description

Technical Field

[0001] The invention relates to the field of planting, and in particular to a planting regulator for inhibiting the thinness and sourness of litchi flesh, and a preparation method and application thereof. Background Art

[0002] High-quality lychee flesh is white and crystal clear, juicy and sweet, rich in vitamin C and iron, especially thick flesh, small core, crisp and sweet flesh, which are deeply loved by consumers. The main factors affecting the quality of lychee are: natural environment, bad weather, such as high temperature, low temperature, strong wind or rain, which will lead to the difficulty of lychee trees to set fruit and low fruit setting rate; human intervention, in order to put lychees on the market early, makes lychees poorly colored and not sweet enough, resulting in poor market feedback. At present, the use of lychee planting regulators can reduce the economic losses caused by the natural environment and human intervention, but the commercially available planting regulators have the problems of wide application of crop types, unclear application period and application amount, poor application effect, high price and high residue, and lack of planting regulators developed for thin lychee flesh and sour flesh. Therefore, the present invention is a kind of planting regulator that inhibits the thinness of lychee flesh and sour flesh, which can improve the quality of lychee and has application prospects. Summary of the invention

[0003] Based on the prior art, the technical problem to be solved by the present invention is to provide a plant regulator for inhibiting the thinness and sourness of litchi pulp, and a preparation method and application thereof.

[0004] The technical solution of the present invention:

[0005] A plant regulator for inhibiting the thinness and sourness of litchi flesh, comprising the following raw materials by weight: 30-35 parts of 6-benzylaminopurine, 8-10 parts of 6-furfurylaminopurine, 5-8 parts of gibberellic acid A4, 3-5 parts of active agent, 3-5 parts of dispersant, 2-3 parts of amino oligosaccharide, and 1-1.5 parts of 28 homobrassinolide.

[0006] Preferably, the active agent is one of sodium dodecylbenzene sulfonate, sodium butylnaphthalene sulfonate, dodecylphenol polyoxyethylene ether and sodium aminobenzene sulfonate.

[0007] Preferably, the dispersant is one of sulfated castor oil, sodium lignin sulfonate and sodium oleoyl polyaminocarboxylate.

[0008] Preferably, the following raw materials are included by weight: 30 parts of 6-benzylaminopurine, 10 parts of 6-furfurylaminopurine, 6 parts of gibberellic acid A4, 3 parts of sodium dodecylbenzenesulfonate, 3 parts of sulfated castor oil, 2 parts of amino oligosaccharides, 2 parts of polyethylene glycol 600 monooleate, and 1 part of 28 homobrassinolide.

[0009] A method for preparing a plant regulator for inhibiting the thinness and sourness of litchi pulp comprises the following steps:

[0010] (1) Weigh 30-35 mg of 6-benzylaminopurine powder, 8-10 mg of 6-furfurylaminopurine, 5-8 mg of gibberellic acid A4, and 1-1.5 mg of 28-homobrassinolide, mix them, add 100 mL of 95-99% ethanol solution, mix at a mixing temperature of 40-45° C., and obtain solution I;

[0011] (2) Weigh 3-5 mg of sodium dodecylbenzene sulfonate powder, 2-3 mg of amino oligosaccharide, and 1-3 mg of polyethylene glycol 600 monooleate, mix them, put them in another container, add 50 mL of purified water and mix them at a mixing temperature of 35-40° C. to obtain solution II;

[0012] (3) Solution I is mixed with solution II, 3 to 5 ml of sulfated castor oil with a mass fraction of 95 to 99% is added, and the mixture is stirred evenly to obtain a finished product.

[0013] The application of this plant regulator in inhibiting the thinness and sourness of litchi flesh is at a volume ratio of 1:1000 to 1:1200 with water. 40 to 50 g of the plant regulator is sprayed per mu. The first spraying is done after the litchi flowers have faded, and the second spraying is done with an interval of 5 to 7 days between the two times.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The plant regulator of the present invention is sprayed on the flower withering period of the litchi tree, and compared with the control example without spraying the plant regulator, the thickness of the litchi pulp, the fructose content, the total sugar ratio and the solid-acid ratio are significantly increased, and the quality of the litchi fruit is increased. The average thickness, large transverse diameter, fructose content, total sugar ratio and solid-acid ratio of the litchi pulp are significantly higher than those without spraying the plant regulator. The average thickness of the pulp of the embodiment is significantly increased by 2.7mm, the large transverse diameter is significantly increased by 1.2mm, the fructose content is significantly increased by 8.8mg / g, the total sugar ratio is significantly increased by 2.1%, and the solid-acid ratio is significantly increased by 17.2%. DETAILED DESCRIPTION

[0016] In order to better understand the technical content of the present invention, the technical solution is clearly and completely described, and specific embodiments are provided below to further illustrate the present invention. The following are only some embodiments of the present invention.

[0017] The raw material components used in this embodiment are benzylaminopurine powder, benzylaminopurine mass fraction 99%, produced by Wuhan Kamik Technology Co., Ltd.; brassinolide powder, brassinolide mass fraction>99%, produced by Xinxiang Runtian Chemical Technology Co., Ltd.; 6-furfurylaminopurine powder, 6-furfurylaminopurine mass fraction 98%, thidiazuron powder, thidiazuron mass fraction ≥95%, Nanjing Dulai Biotechnology Co., Ltd.

[0018] Example 1

[0019] A preparation step of a plant regulator for inhibiting the thinness and sourness of lychee pulp:

[0020] (1) Weigh 30 mg of 6-benzylaminopurine powder, 9 mg of 6-furfurylaminopurine, 5 mg of gibberellic acid A4, and 1 mg of 28-homobrassinolide, add 100 mL of 95% ethanol solution, mix at 40° C., and obtain solution I;

[0021] (2) Weigh 3 mg of sodium dodecylbenzene sulfonate powder, 2 mg of amino oligosaccharide, and 2 mg of polyethylene glycol 600 monooleate, mix them, put them in another container, add 50 mL of purified water, mix at a mixing temperature of 40° C., and obtain solution II;

[0022] (3) Solution I is mixed with solution II, 3 ml of 95% sulfated castor oil is added, and the mixture is stirred evenly to obtain a plant preparation.

[0023] Example 2

[0024] A preparation step of a plant regulator for inhibiting the thinness and sourness of lychee pulp:

[0025] (1) Weigh 35 mg of 6-benzylaminopurine powder, 10 mg of 6-furfurylaminopurine, 7 mg of gibberellic acid A4, and 1.5 mg of 28-homobrassinolide, mix them, add 100 mL of 95% ethanol solution, mix at 40° C., and obtain solution I;

[0026] (2) Weigh 4 mg of sodium dodecylbenzene sulfonate powder, 3 mg of amino oligosaccharide, and 2 mg of Tween 80, mix them, put them in another container, add 50 mL of purified water, mix at a mixing temperature of 40° C., and obtain solution II;

[0027] (3) Solution I is mixed with solution II, 5 ml of 96% sulfated castor oil is added, and the mixture is stirred evenly to obtain a plant preparation.

[0028] Example 3

[0029] A preparation step of a plant regulator for inhibiting the thinness and sourness of lychee pulp:

[0030] (1) Weigh 35 mg of 6-benzylaminopurine powder, 10 mg of 6-furfurylaminopurine, 5 mg of gibberellic acid A4, and 1.5 mg of 28-homobrassinolide, mix them, add 100 mL of 95% ethanol solution, mix at 40° C., and obtain solution I;

[0031] (2) Weigh 3 mg of sodium dodecylbenzene sulfonate powder, 2 mg of amino oligosaccharide, and 2 mg of polyethylene glycol 600 monooleate, put them into another container, add 50 mL of purified water and mix at a mixing temperature of 40° C. to obtain solution II;

[0032] (3) Solution I is mixed with solution II, 3 ml of 95% sulfated castor oil is added, and the mixture is stirred evenly to obtain a plant preparation.

[0033] test

[0034] 16 normally growing litchi trees were randomly selected as test subjects, with 2 trees in each test. The test was sprayed with the reagents of Examples 1 to 3 and Comparative Examples 1 to 3. Control group 1 was sprayed with an equal amount of purified water, and control group 2 was sprayed with an equal mass of commercially available 6-benzylaminopurine plant regulator, produced by Shandong Changyao New Materials Co., Ltd., with a 6-benzylaminopurine content of 99%.

[0035] Application method: The plant regulators of Examples 1 to 3 and Comparative Examples 1 to 3 were diluted with water, and the dilution volume ratio of the plant regulator to water was 1:1000. 45 g was sprayed per mu, and the spraying was carried out after the litchi flowers withered. The spraying was carried out twice in total, and the interval between the first spraying and the second spraying was 7 days.

[0036] Component determination: 15 fruits were randomly selected for the test examples and comparative examples, the pulp was squeezed and the juice was filtered, and the contents of soluble solids (TSS) and titratable acid (TA) were determined. The test method was based on the "Plant Chemical Composition" written by Chen Yegao in 2005.

[0037] Solid acid ratio (%) = TSS / TA × 100%

[0038] Flesh thickness measurement: Peel the lychee and measure the distance from the inner epidermis to the outer epidermis in the cross section.

[0039] Large transverse diameter measurement: Measure with skin on, using a fruit diameter ring. Place the fruit diameter ring on the cross section of the litchi, making sure the fruit fits completely against the inner edge of the fruit diameter ring, then read the value on the fruit diameter ring.

[0040] Comparative Example 1

[0041] The difference between Comparative Example 1 and Example 1 is that the 28-homobrassinolide in the composition for spraying the plant growth regulator on the litchi trees is replaced by an equal mass portion of 24-epicrassinolide.

[0042] Comparative Example 1 The preparation method of the plant regulator comprises the following steps:

[0043] (1) Weigh 30 mg of 6-benzylaminopurine powder, 9 mg of 6-furfurylaminopurine, 5 mg of gibberellic acid A4, and 1 mg of 24-epibrassinolide, add 100 mL of 95% ethanol solution, mix at 40° C., and obtain solution I;

[0044] (2) Weigh 3 mg of sodium dodecylbenzene sulfonate powder, 2 mg of amino oligosaccharide, and 2 mg of polyethylene glycol 600 monooleate, mix them, put them in another container, add 50 mL of purified water, mix at a mixing temperature of 40° C., and obtain solution II;

[0045] (3) Solution I is mixed with solution II, 3 ml of 95% sulfated castor oil is added, and the mixture is stirred evenly to obtain a plant preparation.

[0046] Comparative Example 2

[0047] The difference between Comparative Example 2 and Example 1 is that in the composition of the plant growth regulator sprayed on litchi trees, 28 homobrassinolide and amino oligosaccharide are replaced by 6-benzylaminopurine in equal amounts.

[0048] Comparative Example 2 The preparation method of the plant regulator comprises the following steps:

[0049] (1) Weigh 33 mg of 6-benzylaminopurine powder, 9 mg of 6-furfurylaminopurine, and 5 mg of gibberellic acid A4, mix them, add 100 mL of 95% ethanol solution, mix at 40° C., and obtain solution I;

[0050] (2) Weigh 3 mg of sodium dodecylbenzene sulfonate powder and put it into another container, mix it with 2 mg of polyethylene glycol 600 monooleate, and add 50 mL of purified water and mix at a mixing temperature of 40° C. to obtain solution II;

[0051] (3) Solution I is mixed with solution II, 3 ml of 95% sulfated castor oil is added, and the mixture is stirred evenly to obtain a plant preparation.

[0052] Comparative Example 3

[0053] The difference between Comparative Example 3 and Example 1 is that 6-furfurylaminopurine is removed from the composition of the plant growth regulator sprayed on the litchi trees.

[0054] The preparation method of the plant regulator in comparative example 3 comprises the following steps:

[0055] A preparation step of a plant regulator for inhibiting the thinness and sourness of lychee pulp:

[0056] (1) Weigh 30 mg of 6-benzylaminopurine powder, 5 mg of gibberellic acid A4, and 1 mg of 28-homobrassinolide, add 100 mL of 95% ethanol solution, mix at a mixing temperature of 40° C., and obtain solution I;

[0057] (2) Weigh 3 mg of sodium dodecylbenzene sulfonate powder, 2 mg of amino oligosaccharide, and 2 mg of polyethylene glycol 600 monooleate, mix them, put them in another container, add 50 mL of purified water, mix at a mixing temperature of 40° C., and obtain solution II;

[0058] (3) Solution I is mixed with solution II, 3 ml of 95% sulfated castor oil is added, and the mixture is stirred evenly to obtain a plant preparation.

[0059] Control group

[0060] The control group 1 was subjected to a blank test, in which the litchi trees were not sprayed with any plant regulator but with an equal amount of purified water.

[0061] Control group 2 was subjected to a positive test, in which the litchi trees were sprayed with an equal amount of commercially available 6-benzylaminopurine plant regulator, and the volume ratio of the water was the same as in Example 1.

[0062] Table 1 Litchi index comparison table

[0063] Group Pulp thickness (mm) Large transverse diameter (mm) Fructose (mg / g) Total sugar (%) Solid acid ratio (%) Example 1 6.7a 37.9a 57.3a 16.7a 80.6a Example 2 7.1a 36.9a 60.1a 15.9a 81.7a Example 3 6.9a 37.3a 59.5a 16.4a 79.8a Comparative Example 1 5.2b 37.4a 51.1c 14.1b 72.9b Comparative Example 2 5.4b 37.1a 54.5b 14.9b 75.8b Comparative Example 3 5.8b 37.0a 54.0b 15.1b 76.3b Control group 1 4.2c 36.2b 50.2c 14.2b 63.5d Control group 2 4.6c 36.6b 52.3b 15.0b 70.7c

[0064] Note: a, b, c, d indicate significant differences between different groups (P<0.05)

[0065] The test results show that the average thickness, large transverse diameter, fructose content, total sugar ratio and solid acid ratio of litchi pulp of Examples 1 to 3 are significantly higher than those of Control Group 1 and Control Group 2. Compared with Control Group 1, the average thickness of the pulp of the Example significantly increased by 2.7 mm, the large transverse diameter significantly increased by 1.2 mm, the fructose content significantly increased by 8.8 mg / g, the total sugar ratio significantly increased by 2.1%, and the solid acid ratio significantly increased by 17.2%. Compared with Control Group 2, the average thickness of the pulp of the Example significantly increased by 2.3 mm, the large transverse diameter ratio significantly increased by 0.8 mm, the fructose content significantly increased by 6.7 mg / g, the total sugar ratio significantly increased by 1.3%, and the solid acid ratio significantly increased by 10.0%. The average pulp thickness, fructose content, total sugar ratio and solid acid ratio of the embodiment are also significantly higher than those of comparative examples 1 to 3. Comparison of the average pulp thickness, major transverse diameter, fructose content and total sugar ratio of the lychee in the embodiment with those of the comparative example shows that the average pulp thickness is significantly increased by 1.4 mm, the fructose content is significantly increased by 5.8 mg / g compared with the control group, the total sugar ratio is significantly increased by 1.6% compared with the control group, and the solid acid is significantly increased by 5.7% compared with the control group.

[0066] The results show:

[0067] In summary, the present invention discloses a plant regulator for inhibiting the thinness of litchi flesh and the sourness of the flesh and a method for preparing the same. The main raw materials of the product include 6-benzylaminopurine, brassinolide, 6-furfurylaminopurine and thidiazuron. The product has the effect of significantly increasing the thickness of the flesh and reducing the acidity of litchi fruit, thereby increasing the sweetness of the taste of litchi fruit. The formula of the present invention is sprayed on the litchi fruit after the flowers have faded, and the spraying is for the second time, with a time interval of 5 to 7 days between the two times. The average thickness, large transverse diameter, fructose content, total sugar ratio and solid-acid ratio of the litchi flesh sprayed with the germination agent are significantly higher than those of the control. Compared with not spraying the plant regulator, after spraying the plant regulator of the present invention, the average thickness of the flesh is significantly increased by 2.7 mm, the large transverse diameter is significantly increased by 1.2 mm, the fructose content is significantly increased by 8.8 mg / g, the total sugar ratio is significantly increased by 2.1%, and the solid-acid ratio is significantly increased by 17.2%. The plant regulator of the present invention has a simple production process and good effect, and can improve the quality of litchi fruit.

[0068] The above description is merely an exemplary embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A plant regulator for inhibiting the thinness and sourness of litchi pulp, characterized in that: The raw materials are as follows, calculated by weight: 30 to 35 parts of 6-benzylaminopurine, 8 to 10 parts of 6-furfurylaminopurine, 5 to 8 parts of gibberellic acid A4, 3 to 5 parts of active agent, 3 to 5 parts of dispersant, 2 to 3 parts of amino oligosaccharide, 1 to 3 parts of emulsifier and 1 to 1.5 parts of 28 homobrassinolide.

2. The plant growth regulator according to claim 1, characterized in that: The active agent is one of sodium dodecylbenzene sulfonate, sodium butylnaphthalene sulfonate, dodecylphenol polyoxyethylene ether and sodium aminobenzene sulfonate.

3. The plant growth regulator according to claim 1, characterized in that: The dispersant is one of sulfated castor oil, sodium lignin sulfonate and sodium oleoyl polyaminocarboxylate.

4. The plant growth regulator according to claim 1, characterized in that: The emulsifier is one of polyethylene glycol 600 monooleate and Tween 80.

5. The plant growth regulator according to claim 1, characterized in that: The invention comprises the following raw materials by weight: 30 parts of 6-benzylaminopurine, 10 parts of 6-furfurylaminopurine, 6 parts of gibberellic acid A4, 3 parts of sodium dodecylbenzenesulfonate, 3 parts of sulfated castor oil, 2 parts of amino oligosaccharides, 2 parts of polyethylene glycol 600 monooleate and 1 part of 28 homobrassinolide.

6. A method for preparing a plant regulator for inhibiting the thinness and sourness of litchi pulp, characterized in that: The preparation steps include: (1) Weigh 30-35 mg of 6-benzylaminopurine powder, 8-10 mg of 6-furfurylaminopurine, 5-8 mg of gibberellic acid A4, and 1-1.5 mg of 28-homobrassinolide, mix them, add 100 mL of 95-99% ethanol solution, mix at a mixing temperature of 40-45° C., and obtain solution I; (2) Weigh 3-5 mg of sodium dodecylbenzene sulfonate powder, 2-3 mg of amino oligosaccharide, and 1-3 mg of polyethylene glycol 600 monooleate, mix them, put them in another container, add 50 mL of purified water and mix them at a mixing temperature of 35-40° C. to obtain solution II; (3) Solution I is mixed with solution II, 3 to 5 ml of sulfated castor oil with a mass fraction of 95 to 99% is added, and the mixture is stirred evenly to obtain a finished product.

7. Use of the plant regulator according to any one of claims 1 to 4 in inhibiting the thinness and sourness of litchi flesh.

8. Use of the plant regulator prepared by the preparation method of claim 5 in inhibiting the thinness and sourness of litchi pulp.

9. The use according to claim 7 or 8, characterized in that: The volume ratio of the plant regulator to water is 1:1000-1:1200, and 40-50g of the plant regulator is sprayed per mu. The first spraying is done after the litchi flowers have withered, and the second spraying is done with an interval of 5-7 days between the two sprayings.