Formula of pet food for strengthening intestines and balancing nutrition for pets

Abstract

The invention relates to the technical field of pet food, and particularly discloses a pet food formula for strengthening intestines and balancing nutrition for pets, the pet food formula comprises the following raw materials: meat, vegetables, fruits, whole goat milk powder, Maillard reaction products, composite algae powder, yucca powder and synbiotics microcapsules; the preparation method comprises the following steps: respectively crushing the meat, the vegetables and the fruits; stirring and mixing the meat paste, the vegetable paste, the fruit paste, the whole goat milk powder, the composite algae powder, the yucca powder and the synbiotics microcapsules to obtain a mixed material; placing the mixed material in an extruder to prepare cylindrical particles; spraying the Maillard reaction product on the surfaces of the particles by adopting vacuum spraying equipment; the sprayed particles are fed into a freeze-drying bin to be freeze-dried; and packaging the frozen freeze-dried particles into bags to obtain the pet food capable of strengthening the intestines and being balanced in nutrition. The formula of the pet food capable of strengthening the intestines and balancing the nutrition has remarkable effects in the aspects of improving the health of the intestines, guaranteeing the balanced nutrition, improving the appetite of the pets and the like.

Description

Technical Field

[0001] The present application relates to the technical field of pet food, and more specifically, to a pet food formula that is intestinal-friendly and nutritionally balanced for pets. Background Art

[0002] Pets play an increasingly important role in modern society. They are not only members of the family, but also supporters of emotional sustenance and mental health for many people. The status of pets in people's hearts is becoming increasingly important, and their health has also received widespread attention. At the same time, people's requirements for the quality of pet food are becoming more and more picky and stringent.

[0003] As an important part of the overall health of pets, intestinal health has become the focus of pet owners. The main way to obtain nutrition is through the intestines, and the intestines are also more prone to problems than other organs. Pet intestinal problems mainly include gastrointestinal inflammation, food intolerance, constipation and diarrhea, etc. These problems not only affect the daily quality of life of pets, but may also cause more serious health problems.

[0004] Pet food is an indispensable part of pets' daily life. It provides pets with necessary nutrition and energy, and maintains their life activities and health. Since most pets face intestinal problems, whether pet food has intestinal health effects has attracted widespread attention. Based on the above statement, the present application provides a pet food formula for intestinal health and balanced nutrition for pets. Summary of the invention

[0005] In order to solve the above technical problems, the present application provides a pet food formula that is intestinal-strengthening and nutritionally balanced for pets.

[0006] In the first aspect, the present application provides a pet food formula for pets that is intestinal-strengthening and nutritionally balanced, using the following technical solution:

[0007] A pet food formula for strengthening the intestines of pets and having balanced nutrition, comprising the following raw materials in parts by weight: 60-80 parts of meat, 10-20 parts of vegetables, 5-10 parts of fruits, 2-4 parts of whole goat milk powder, 2-4 parts of Maillard reaction products, 2-4 parts of composite algae powder, 0.5-1.5 parts of yucca powder, and 0.4-0.8 parts of synbiotic microcapsules.

[0008] Preferably, the meat is a mixture of chicken breast, beef and shrimp in a mass ratio of 3-5:1-2:1, the vegetables are a mixture of spinach, parsley and green cabbage in a mass ratio of 1:0.8-1.2:0.8-1.2, and the fruit is a mixture of apples, cranberries and blueberries in a mass ratio of 4-8:0.8-1.2:0.8-1.2.

[0009] Preferably, the preparation method of the Maillard reaction product is as follows:

[0010] 3. The preparation method of the Maillard reaction product is as follows:

[0011] A1. Grind alfalfa and pine needles in a grinder respectively, and pass through an 80-100 mesh sieve to obtain alfalfa powder and pine needle powder;

[0012] A2, placing alfalfa powder and pine needle powder in distilled water, adding compound enzyme 1 for enzymolysis to obtain an enzymolysis solution, and concentrating the enzymolysis solution by rotary evaporation to a water content of 10-15%, thereby obtaining an enzymolysis product;

[0013] A3. Mix the enzymatic hydrolysate and reducing sugar, adjust the pH to 4-6, adjust the temperature to 80-100°C, react for 0.8-1.2h, filter and dry, and crush through a 80-120 mesh sieve to obtain the desired Maillard reaction product.

[0014] Preferably, in step A2, the mass ratio of alfalfa powder, pine needle powder, distilled water and compound enzyme 1 is 3-5:1-2:10-14:0.2-0.6; the compound enzyme 1 is a mixture of trypsin and cellulase in a mass ratio of 3-5:0.8-1.2.

[0015] Preferably, in step A3, the mass ratio of the enzymatic hydrolysate to the reducing sugar is 3-5:0.2-0.6; and the reducing sugar is at least one of glucose, fructose and galactose.

[0016] Preferably, the preparation method of the composite algae powder is as follows:

[0017] B1. Mixing Spirulina, Haematococcus pluvialis and Chlorella pyrenoidosa, and then placing the mixture in distilled water for ultrasonic cleaning at a frequency of 20-40 kHz to remove impurities, and then washing the mixture with distilled water for 3-5 times after the ultrasonic cleaning, and drying the mixture in an oven at 50-60° C. to obtain an algae mixture;

[0018] B2, crushing the algae mixture in a grinder, and passing through an 80-120 mesh sieve to obtain algae powder 1;

[0019] B3, placing algae powder 1, distilled water and compound enzyme 2 in a reaction container, adjusting the pH to 5-7, stirring and enzymolyzing at 40-50° C. for 2-4 hours, and then filtering, taking the filter cake, washing it with distilled water for 3-5 times, and then drying it to obtain algae powder 2;

[0020] B4. Place algae powder 2 in high temperature steam for sterilization for 2-3 hours, and cool to obtain the desired composite algae powder.

[0021] Preferably, in step B1, the mass ratio of Spirulina, Haematococcus pluvialis, Chlorella pyrenoidosa and distilled water is 5-8:2-4:1-2:20-30; in step B3, the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:12-16:0.06-0.08, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 2-3:1-2.

[0022] Preferably, the preparation method of the synbiotic microcapsules is as follows:

[0023] C1. Culturing probiotics in a culture medium to obtain a probiotic solution;

[0024] C2, mixing the probiotic solution, the prebiotic, a sodium carboxymethyl cellulose solution with a mass concentration of 3-5%, and insoluble calcium salt particles to obtain a mixed solution 1;

[0025] C3, dispersing the mixed solution 1 in soybean oil, stirring and mixing to form a water-in-oil emulsion, then adding a citric acid solution with a mass concentration of 5-10%, stirring and reacting for 0.5-1h, eluting, and collecting by centrifugation to obtain the synbiotic microcapsules.

[0026] Preferably, the probiotics in step C1 are composed of Lactobacillus mucosa, Saccharomyces boulardii and Bacillus coagulans, and the number of viable Lactobacillus mucosa in the probiotic solution is ≥ 1×10 8 CFU / g, the number of live bacteria of Saccharomyces boulardii ≥ 1×10 8 CFU / g, the number of live Bacillus coagulans ≥ 2×10 7 CFU / g; the prebiotic in step C2 is at least one of oligofructose, oligogalactose and inulin; and the insoluble calcium salt particles are calcium carbonate or calcium phosphate.

[0027] Preferably, in step C2, the mass ratio of the probiotic solution, prebiotics, sodium carboxymethyl cellulose solution, and insoluble calcium salt particles is 1:5-7:10-14:0.8-1.2; and in step C3, the mass ratio of the mixed solution 1, soybean oil, and citric acid solution is 1:4-6:0.4-0.8.

[0028] In a second aspect, the present application provides a method for preparing a pet food that is intestinal-strengthening and nutritionally balanced for pets, using the following technical solution:

[0029] A method for preparing intestinal-strengthening and nutritionally balanced pet food for pets comprises the following steps:

[0030] S1, crushing meat, vegetables and fruits respectively to obtain meat puree, vegetable puree and fruit puree;

[0031] S2, placing meat puree, vegetable puree, fruit puree, whole goat milk powder, compound algae powder, yucca powder, and synbiotics microcapsules in a blender, stirring and mixing to obtain a mixed material;

[0032] S3, placing the mixed material in an extruder to form cylindrical particles with a length of 3-5 cm and a diameter of 1-2 cm;

[0033] S4, using vacuum spraying equipment to spray the Maillard reaction product on the particle surface;

[0034] S5. After spraying, the cylindrical particles are sent to a freeze-drying chamber and subjected to negative pressure freeze drying at -30--50°C for 20-30 hours until the moisture content is reduced to 4-6%, and the freeze-dried particles are taken out;

[0035] S6. Pack the freeze-dried particles into bags, and vacuum and fill with nitrogen when packaging the products, so as to finally obtain pet food that is intestinal-friendly and nutritionally balanced.

[0036] In summary, this application has the following beneficial effects:

[0037] 1. The present application crushes alfalfa and pine needles and performs enzymolysis to obtain an enzymolysate, and adds reducing sugar to the enzymolysate to cause Maillard reaction to obtain a Maillard reaction product. Amino acids and peptides in alfalfa and pine needles react with reducing sugar to form a unique flavor, making the pet food richer in taste and flavor. Alfalfa and pine needles have certain synergistic effects in terms of nutrients, flavor and functionality, and their combined use can further improve the flavor quality and nutritional value of pet food.

[0038] At the same time, the Maillard reaction products contain substances with antioxidant and anti-inflammatory effects, which help protect pet cells from damage caused by oxidative stress and inflammatory responses. The Maillard reaction products also contain some substances with prebiotic properties, which help promote the growth and reproduction of beneficial bacteria in the pet's intestines and inhibit the activity of harmful bacteria, thereby maintaining the balance of intestinal microecology.

[0039] 2. In the present application, spirulina, Haematococcus pluvialis, and Chlorella vulgaris are mixed and crushed, compound enzyme 2 is added for enzymolysis, and then placed in high-temperature steam for sterilization to obtain algae powder. Compound enzyme 2 is a mixture of cellulase and pectinase. Cellulase degrades cellulose, and pectinase degrades pectin. After enzymolysis, compound enzyme 2 can release nutrients in algae cells to a greater extent, which is more conducive to subsequent digestion and absorption by pets.

[0040] Spirulina protein in spirulina can promote the repair, regeneration and normal secretion of epithelial cells in the digestive tract, effectively prevent the degeneration, apoptosis and reduction of gastric mucosal epithelial cells, protect the gastric mucosa, and polysaccharides and phycocyanin help improve the body's immune function and help resist diseases and infections. Haematococcus pluvialis is rich in astaxanthin, which has strong antioxidant capacity, and can prevent diseases caused by oxidative stress, such as cardiovascular disease; at the same time, Haematococcus pluvialis is rich in dietary fiber, which helps promote intestinal peristalsis, accelerates metabolism in the body, and helps to moisturize the intestines and relieve constipation. Protein-core chlorella can promote the absorption of nutrients and has certain effects of lowering blood lipids and blood pressure; at the same time, protein-core chlorella has prebiotic ingredients that can maintain the balance of intestinal flora and improve digestive function. Adding the algae powder prepared in this application to pet food can provide pets with comprehensive and balanced nutrition, enhance immunity, and promote intestinal health.

[0041] 3. The present application additionally adds yucca powder. The active ingredients in yucca powder can promote immune function and enhance the immune response of pets. The yucca saponins in yucca powder can promote the healthy growth of pet skin and hair and reduce hair loss. Yucca powder can greatly improve the absorption and digestibility of pets' stomachs and intestines, effectively reduce the production of ammonia in feces and urine and intestinal tract, and improve the problem of pet stool odor. Yucca powder can maintain the acid-base balance of the intestine, help protect the balance of intestinal flora and the stability of pH value, inhibit the growth of harmful bacteria in the intestine, and promote the reproduction of intestinal probiotics, thereby maintaining the balance of intestinal flora.

[0042] 4. The present application mixes probiotics and prebiotics, and then adopts microcapsule coating to obtain synbiotic microcapsules. Probiotics are composed of mucosal lactobacillus, boulardii yeast and bacillus coagulans. As a beneficial bacteria, mucosal lactobacillus can improve the imbalance of intestinal flora and restore the balance of intestinal flora. Boulardii yeast can reduce the number of harmful bacteria and promote the growth of beneficial microorganisms by competitive inhibition and production of organic acids, thereby maintaining the healthy state of the intestine. Bacillus coagulans increases the acidity of the intestinal environment by producing lactic acid, inhibits the growth of pathogens, regulates the balance of intestinal flora, and maintains intestinal health. The three are mixed together to maintain the balance of intestinal flora, promote food digestion and absorption, relieve constipation, and reduce intestinal inflammation, which has a significant effect on intestinal health. Further adopting microcapsule coating technology can ensure that the probiotics maintain a high number of live bacteria during production and storage, thereby improving the utilization rate of probiotics; at the same time, the release time and amount of probiotics can be better controlled, thereby optimizing the effect of probiotics in the intestine.

[0043] 5. The intestinal-strengthening and nutritionally balanced pet food prepared in this application has significant beneficial effects in improving intestinal health, ensuring nutritional balance, and increasing pet appetite. DETAILED DESCRIPTION

[0044] The present application is further described in detail below with reference to the embodiments.

[0045] The raw materials used in the examples and comparative examples of the present application are as follows:

[0046] Cellulase was purchased from MacLean Reagent Company, catalog number C1794;

[0047] Trypsin was purchased from MacLean Reagent Company, catalog number T2600000;

[0048] Pectinase was purchased from MacLean Reagent Company, catalog number P2401;

[0049] Lactobacillus mucosa was purchased from China General Microbiological Culture Collection Center, with the collection number CGMCC 1.15986;

[0050] Saccharomyces boulardii was purchased from Hubei Yamade Biopharmaceutical Co., Ltd.;

[0051] Bacillus coagulans was purchased from China General Microbiological Culture Collection Center with the collection number CGMCC 1.10823.

[0052] Embodiments 1-3 provide a pet food formula for pets that is intestinal-friendly and nutritionally balanced.

[0053] Example 1

[0054] A pet food formula for strengthening the pet's intestines and having balanced nutrition, comprising the following raw materials in parts by weight: 60 parts of meat, 10 parts of vegetables, 5 parts of fruit, 2 parts of whole goat milk powder, 2 parts of Maillard reaction product, 2 parts of compound algae powder, 0.5 parts of yucca powder, and 0.4 parts of synbiotic microcapsules.

[0055] Among them, the meat is a mixture of chicken breast, beef and shrimp in a mass ratio of 3:1:1, the vegetables are a mixture of spinach, parsley and green cabbage in a mass ratio of 1:0.8:0.8, and the fruit is a mixture of apples, cranberries and blueberries in a mass ratio of 4:0.8:0.8.

[0056] The preparation method of the Maillard reaction product is as follows:

[0057] A1. Grind alfalfa and pine needles in a grinder respectively, and pass through an 80-mesh sieve to obtain alfalfa powder and pine needle powder;

[0058] A2, placing alfalfa powder and pine needle powder in distilled water, stirring and dispersing at a rate of 80 rpm for 0.2 h, heating to 40°C, adjusting the pH to 5, adding compound enzyme 1, stirring and hydrolyzing at a rate of 40 rpm for 24 h to obtain an enzymolysis solution; wherein the mass ratio of alfalfa powder, pine needle powder, distilled water, and compound enzyme 1 is 3:1:10:0.2; the compound enzyme 1 is trypsin and cellulase in a mass ratio of 3:0.8; the enzymolysis solution is concentrated by rotary evaporation to a water content of 10% to obtain an enzymolysis product;

[0059] A3. Stir and disperse the enzymolysate and glucose at a rate of 80 rpm for 0.2 h, adjust the pH to 4, adjust the temperature to 80° C., react for 0.8 h, filter and dry, and crush through an 80-mesh sieve to obtain the desired Maillard reaction product; wherein the mass ratio of enzymolysate to glucose is 3:0.2.

[0060] The preparation method of the composite algae powder is as follows:

[0061] B1. Mixing Spirulina, Haematococcus pluvialis and Chlorella pyrenoidosa, placing the mixture in distilled water and ultrasonically cleaning at a frequency of 20 kHz to remove impurities, washing with distilled water for 3 times after the ultrasonic cleaning, and drying in an oven at 50° C. to obtain an algae mixture; wherein the mass ratio of Spirulina, Haematococcus pluvialis, Chlorella pyrenoidosa and distilled water is 5:2:1:20;

[0062] B2, crushing the algae mixture in a grinder, and passing through an 80-mesh sieve to obtain algae powder 1;

[0063] B3. Place algae powder 1, distilled water and compound enzyme 2 in a reaction container, adjust the pH to 5, and perform enzymolysis at 40 rpm at 40°C for 2 hours, then filter. Take the filter cake, wash it with distilled water 3 times, and dry it in an oven at 50°C to obtain algae powder 2; wherein the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:12:0.06, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 2:1.

[0064] B4. Place algae powder 2 in 100°C high-temperature steam sterilization for 2 hours, cool to room temperature, and obtain the desired composite algae powder.

[0065] The preparation method of synbiotic microcapsules is as follows:

[0066] C1. Preparation of YPD liquid medium: weigh 20 g glucose, 20 g peptone, 10 g yeast powder, add distilled water to 1 L, adjust the pH to 7, and sterilize at 115°C for 20 min;

[0067] C2. Lactobacillus mucosa, Saccharomyces boulardii and Bacillus coagulans were cultured in YPD liquid medium respectively to obtain Lactobacillus mucosa liquid, Saccharomyces boulardii liquid and Bacillus coagulans liquid, which were mixed to obtain probiotic liquid, wherein the number of viable Lactobacillus mucosa in the probiotic liquid was 1×10 8 CFU / g, the number of live bacteria of Saccharomyces boulardii is 1×10 8 CFU / g, the number of live Bacillus coagulans is 2×10 7 CFU / g;

[0068] C3. Mix the probiotic solution, oligofructose, 3% sodium carboxymethyl cellulose solution, and calcium carbonate to obtain a mixed solution 1; wherein the mass ratio of the probiotic solution, oligofructose, sodium carboxymethyl cellulose solution, and calcium carbonate is 1:5:10:0.8.

[0069] C4. Disperse the mixed solution 1 in soybean oil, stir and mix at a rate of 40 rpm for 0.2 h to form an oil-in-water emulsion, then add a citric acid solution with a mass concentration of 5%, stir and react at a rate of 40 rpm for 0.5 h, elute, and collect by centrifugation at 3000 rpm for 4 min to obtain the synbiotic microcapsules, wherein the mass ratio of the mixed solution 1, soybean oil and citric acid solution is 1:4:0.4.

[0070] A method for preparing a pet food that is intestinal-strengthening and nutritionally balanced for pets is as follows:

[0071] S1. Grind meat, vegetables and fruits respectively and pass them through a 40-mesh sieve to obtain meat puree, vegetable puree and fruit puree;

[0072] S2, placing meat puree, vegetable puree, fruit puree, whole goat milk powder, compound algae powder, yucca powder, and synbiotics microcapsules in a blender, and stirring and mixing at a speed of 200 rpm for 3 h to obtain a mixed material;

[0073] S3, placing the mixed material in an extruder to form cylindrical particles with a length of 3 cm and a diameter of 1 cm; wherein the extruder speed is 120 rpm and the extrusion temperature is 140°C;

[0074] S4, using vacuum spraying equipment to spray the Maillard reaction product on the particle surface;

[0075] S5. After spraying, the cylindrical particles are sent to a freeze-drying chamber and subjected to negative pressure freeze drying at -30°C for 20 hours until the moisture content is reduced to 4%, and the freeze-dried particles are taken out;

[0076] S6. Pack the freeze-dried particles into bags, and vacuum and fill with nitrogen when packaging the products, so as to finally obtain pet food that is intestinal-friendly and nutritionally balanced.

[0077] Example 2

[0078] A pet food formula for strengthening the pet's intestines and having balanced nutrition, comprising the following raw materials in parts by weight: 70 parts of meat, 15 parts of vegetables, 7.5 parts of fruit, 3 parts of whole goat milk powder, 3 parts of Maillard reaction product, 3 parts of compound algae powder, 1 part of yucca powder, and 0.6 parts of synbiotic microcapsules.

[0079] Among them, the meat is a mixture of chicken breast, beef and shrimp in a mass ratio of 4:1.5:1; the vegetables are a mixture of spinach, parsley and green cabbage in a mass ratio of 1:1:1; and the fruit is a mixture of apples, cranberries and blueberries in a mass ratio of 6:1:1.

[0080] The preparation method of the Maillard reaction product is as follows:

[0081] A1. Grind alfalfa and pine needles in a grinder respectively, and pass through a 90-mesh sieve to obtain alfalfa powder and pine needle powder;

[0082] A2, placing alfalfa powder and pine needle powder in distilled water, stirring and dispersing at a rate of 80 rpm for 0.2 h, heating to 40°C, adjusting the pH to 5, adding compound enzyme 1, stirring and hydrolyzing at a rate of 40 rpm for 24 h to obtain an enzymolysis solution; wherein the mass ratio of alfalfa powder, pine needle powder, distilled water, and compound enzyme 1 is 4:1.5:12:0.4; the compound enzyme 1 is trypsin and cellulase in a mass ratio of 4:1; the enzymolysis solution is concentrated by rotary evaporation to a water content of 12.5% ​​to obtain an enzymolysis product;

[0083] A3. Stir and disperse the enzymolysate and glucose at a rate of 80 rpm for 0.2 h, adjust the pH to 5, adjust the temperature to 90° C., react for 1 h, filter and dry, and crush through a 100-mesh sieve to obtain the desired Maillard reaction product; wherein the mass ratio of the enzymolysate to glucose is 4:0.4.

[0084] The preparation method of the composite algae powder is as follows:

[0085] B1. Mixing Spirulina, Haematococcus pluvialis and Chlorella pyrenoidosa, placing the mixture in distilled water and ultrasonically cleaning at a frequency of 30 kHz to remove impurities, washing with distilled water for 4 times after the ultrasonic cleaning, and drying in an oven at 55° C. to obtain an algae mixture; wherein the mass ratio of Spirulina, Haematococcus pluvialis, Chlorella pyrenoidosa and distilled water is 6.5:3:1.5:25;

[0086] B2, crushing the algae mixture in a grinder, and passing through a 100-mesh sieve to obtain algae powder 1;

[0087] B3. Place algae powder 1, distilled water and compound enzyme 2 in a reaction container, adjust the pH to 6, and perform enzymolysis at 45°C and 40 rpm for 2 hours, then filter. Take the filter cake, wash it with distilled water 3 times, and dry it in an oven at 50°C to obtain algae powder 2; wherein the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:14:0.07, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 2.5:1.5.

[0088] B4. Place algae powder 2 in 100°C high-temperature steam sterilization for 2.5 hours, cool to room temperature, and obtain the desired composite algae powder.

[0089] The preparation method of synbiotic microcapsules is as follows:

[0090] C1. Preparation of YPD liquid medium: weigh 20 g glucose, 20 g peptone, 10 g yeast powder, add distilled water to 1 L, adjust the pH to 7, and sterilize at 115°C for 20 min;

[0091] C2. Lactobacillus mucosa, Saccharomyces boulardii and Bacillus coagulans were cultured in YPD liquid medium respectively to obtain Lactobacillus mucosa liquid, Saccharomyces boulardii liquid and Bacillus coagulans liquid, which were mixed to obtain probiotic liquid, wherein the number of viable Lactobacillus mucosa in the probiotic liquid was 1×10 8 CFU / g, the number of live bacteria of Saccharomyces boulardii is 1×10 8 CFU / g, the number of live Bacillus coagulans is 2×10 7 CFU / g;

[0092] C3. Mix the probiotic solution, inulin, a sodium carboxymethyl cellulose solution with a mass concentration of 3%, and calcium carbonate to obtain a mixed solution 1; wherein the mass ratio of the probiotic solution, inulin, sodium carboxymethyl cellulose solution, and calcium carbonate is 1:6:12:1.

[0093] C4. Disperse the mixed solution 1 in soybean oil, stir and mix at a rate of 40 rpm for 0.2 h to form an oil-in-water emulsion, then add a citric acid solution with a mass concentration of 7.5%, stir and react at a rate of 40 rpm for 0.75 h, elute, and collect by centrifugation at 3000 rpm for 4 min to obtain the synbiotic microcapsules, wherein the mass ratio of the mixed solution 1, soybean oil and citric acid solution is 1:5:0.6.

[0094] A method for preparing a pet food that is intestinal-strengthening and nutritionally balanced for pets is as follows:

[0095] S1. Grind meat, vegetables and fruits respectively and pass them through a 40-mesh sieve to obtain meat puree, vegetable puree and fruit puree;

[0096] S2, placing meat puree, vegetable puree, fruit puree, whole goat milk powder, compound algae powder, yucca powder, and synbiotics microcapsules in a blender, and stirring and mixing at a speed of 200 rpm for 3 h to obtain a mixed material;

[0097] S3, placing the mixed material in an extruder to form cylindrical particles with a length of 4 cm and a diameter of 1.5 cm; wherein the extruder speed is 120 rpm and the extrusion temperature is 140°C;

[0098] S4, using vacuum spraying equipment to spray the Maillard reaction product on the particle surface;

[0099] S5. After spraying, the cylindrical particles are sent to a freeze-drying chamber and freeze-dried under negative pressure at -40°C for 25 hours until the moisture content is reduced to 5%, and the freeze-dried particles are taken out;

[0100] S6. Pack the freeze-dried particles into bags, and vacuum and fill with nitrogen when packaging the products, so as to finally obtain pet food that is intestinal-friendly and nutritionally balanced.

[0101] Example 3

[0102] A pet food formula for strengthening the pet's intestines and having balanced nutrition, comprising the following raw materials in parts by weight: 80 parts of meat, 20 parts of vegetables, 10 parts of fruit, 4 parts of whole goat milk powder, 4 parts of Maillard reaction product, 4 parts of compound algae powder, 1.5 parts of yucca powder, and 0.8 parts of synbiotic microcapsules.

[0103] Among them, the meat is a mixture of chicken breast, beef and shrimp in a mass ratio of 5:2:1; the vegetables are a mixture of spinach, parsley and green cabbage in a mass ratio of 1:1.2:1.2; and the fruit is a mixture of apples, cranberries and blueberries in a mass ratio of 8:1.2:1.2.

[0104] The preparation method of the Maillard reaction product is as follows:

[0105] A1. Grind alfalfa and pine needles in a grinder respectively, and pass through a 100-mesh sieve to obtain alfalfa powder and pine needle powder;

[0106] A2, placing alfalfa powder and pine needle powder in distilled water, stirring and dispersing at a rate of 80 rpm for 0.2 h, heating to 40°C, adjusting the pH to 5, adding compound enzyme 1, stirring and hydrolyzing at a rate of 40 rpm for 24 h to obtain an enzymolysis solution; wherein the mass ratio of alfalfa powder, pine needle powder, distilled water, and compound enzyme 1 is 5:2:14:0.6; the compound enzyme 1 is trypsin and cellulase in a mass ratio of 5:1.2; the enzymolysis solution is concentrated by rotary evaporation to a water content of 15% to obtain an enzymolysis product;

[0107] A3. Stir and disperse the hydrolysate and galactose at a rate of 80 rpm for 0.2 h, adjust the pH to 6, adjust the temperature to 100° C., react for 1.2 h, filter and dry, and crush through a 120-mesh sieve to obtain the desired Maillard reaction product; wherein the mass ratio of the hydrolysate to galactose is 5:0.6.

[0108] The preparation method of the composite algae powder is as follows:

[0109] B1. Mixing Spirulina, Haematococcus pluvialis and Chlorella pyrenoidosa, placing the mixture in distilled water and ultrasonically cleaning at a frequency of 40 kHz to remove impurities, washing with distilled water for 5 times after the ultrasonic cleaning, and drying in an oven at 60° C. to obtain an algae mixture; wherein the mass ratio of Spirulina, Haematococcus pluvialis, Chlorella pyrenoidosa and distilled water is 8:4:2:30;

[0110] B2, crushing the algae mixture in a grinder, and passing through a 120-mesh sieve to obtain algae powder 1;

[0111] B3. Place algae powder 1, distilled water and compound enzyme 2 in a reaction container, adjust the pH to 7, and perform enzymolysis at 50°C and 40 rpm for 2 hours, then filter. Take the filter cake, wash it with distilled water 3 times, and dry it in an oven at 50°C to obtain algae powder 2; wherein the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:16:0.08, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 3:2.

[0112] B4. Place algae powder 2 in 100°C high-temperature steam sterilization for 3 hours, cool to room temperature, and obtain the desired composite algae powder.

[0113] The preparation method of synbiotic microcapsules is as follows:

[0114] C1. Preparation of YPD liquid medium: weigh 20 g glucose, 20 g peptone, 10 g yeast powder, add distilled water to 1 L, adjust the pH to 7, and sterilize at 115°C for 20 min;

[0115] C2. Lactobacillus mucosa, Saccharomyces boulardii and Bacillus coagulans were cultured in YPD liquid medium respectively to obtain Lactobacillus mucosa liquid, Saccharomyces boulardii liquid and Bacillus coagulans liquid, which were mixed to obtain probiotic liquid, wherein the number of viable Lactobacillus mucosa in the probiotic liquid was 1×10 8 CFU / g, the number of live bacteria of Saccharomyces boulardii is 1×10 8 CFU / g, the number of live Bacillus coagulans is 2×10 7 CFU / g;

[0116] C3. Mix the probiotic solution, galacto-oligosaccharide, 3% sodium carboxymethyl cellulose solution, and calcium phosphate to obtain a mixed solution 1; wherein the mass ratio of the probiotic solution, galacto-oligosaccharide, sodium carboxymethyl cellulose solution, and calcium phosphate is 1:7:14:1.2.

[0117] C4. Disperse the mixed solution 1 in soybean oil, stir and mix at a rate of 40 rpm for 0.2 h to form an oil-in-water emulsion, then add a citric acid solution with a mass concentration of 10%, stir and react at a rate of 40 rpm for 1 h, elute, and collect by centrifugation at 3000 rpm for 4 min to obtain the synbiotic microcapsules, wherein the mass ratio of the mixed solution 1, soybean oil and citric acid solution is 1:6:0.8.

[0118] A method for preparing a pet food that is intestinal-strengthening and nutritionally balanced for pets is as follows:

[0119] S1. Grind meat, vegetables and fruits respectively and pass them through a 40-mesh sieve to obtain meat puree, vegetable puree and fruit puree;

[0120] S2, placing meat puree, vegetable puree, fruit puree, whole goat milk powder, compound algae powder, yucca powder, and synbiotics microcapsules in a blender, and stirring and mixing at a speed of 200 rpm for 3 h to obtain a mixed material;

[0121] S3, placing the mixed material in an extruder to form cylindrical particles with a length of 5 cm and a diameter of 2 cm; wherein the extruder speed is 120 rpm and the extrusion temperature is 140°C;

[0122] S4, using vacuum spraying equipment to spray the Maillard reaction product on the particle surface;

[0123] S5. After spraying, the cylindrical particles are sent to a freeze-drying chamber and freeze-dried under negative pressure at -50°C for 30 hours until the moisture content is reduced to 6%, and the freeze-dried particles are taken out;

[0124] S6. Pack the freeze-dried particles into bags, and vacuum and fill with nitrogen when packaging the products, so as to finally obtain pet food that is intestinal-friendly and nutritionally balanced.

[0125] In order to verify the comprehensive performance of the intestinal-strengthening and nutritionally balanced pet food prepared in Examples 1-3 of the present application, the applicant set up comparative examples 1-12, which are as follows:

[0126] Comparative Example 1

[0127] Comparative Example 1 is the same as Example 1, except that the Maillard reaction product is prepared only from alfalfa, as follows:

[0128] The preparation method of the Maillard reaction product is as follows:

[0129] A1. Grind alfalfa in a grinder and pass it through an 80-mesh sieve to obtain alfalfa powder;

[0130] A2, placing alfalfa powder in distilled water, stirring and dispersing at a rate of 80 rpm for 0.2 h, heating to 40°C, adjusting the pH to 5, adding compound enzyme 1, stirring and hydrolyzing at a rate of 40 rpm for 24 h to obtain an enzymolysis solution; wherein the mass ratio of alfalfa powder, distilled water, and compound enzyme 1 is 4:10:0.2; the compound enzyme 1 is trypsin and cellulase in a mass ratio of 3:0.8; the enzymolysis solution is concentrated by rotary evaporation to a moisture content of 10% to obtain an enzymolysis product;

[0131] A3. Stir and disperse the enzymolysate and glucose at a rate of 80 rpm for 0.2 h, adjust the pH to 4, adjust the temperature to 80° C., react for 0.8 h, filter and dry, and crush through an 80-mesh sieve to obtain the desired Maillard reaction product; wherein the mass ratio of enzymolysate to glucose is 3:0.2.

[0132] Comparative Example 2

[0133] Comparative Example 2 is the same as Example 1, except that the Maillard reaction product is prepared only from pine needles, as follows:

[0134] The preparation method of the Maillard reaction product is as follows:

[0135] A1. Pine needles were crushed in a grinder and passed through an 80-mesh sieve to obtain pine needle powder;

[0136] A2, placing pine needle powder in distilled water, stirring and dispersing at a rate of 80 rpm for 0.2 h, heating to 40°C, adjusting the pH to 5, adding compound enzyme 1, stirring and hydrolyzing at a rate of 40 rpm for 24 h to obtain an enzymolysis solution; wherein the mass ratio of pine needle powder, distilled water, and compound enzyme 1 is 4:10:0.2; the compound enzyme 1 is trypsin and cellulase in a mass ratio of 3:0.8; the enzymolysis solution is concentrated by rotary evaporation to a water content of 10% to obtain an enzymolysis product;

[0137] A3. Stir and disperse the enzymolysate and glucose at a rate of 80 rpm for 0.2 h, adjust the pH to 4, adjust the temperature to 80° C., react for 0.8 h, filter and dry, and crush through an 80-mesh sieve to obtain the desired Maillard reaction product; wherein the mass ratio of enzymolysate to glucose is 3:0.2.

[0138] Comparative Example 3

[0139] Comparative Example 3 is the same as Example 1, except that the Maillard reaction product is replaced by alfalfa pine needle hydrolysate, as follows:

[0140] The preparation method of alfalfa pine needle hydrolysate is as follows:

[0141] A1. Grind alfalfa and pine needles in a grinder respectively, and pass through an 80-mesh sieve to obtain alfalfa powder and pine needle powder;

[0142] A2. Place alfalfa powder and pine needle powder in distilled water, stir and disperse at a rate of 80 rpm for 0.2 h, heat to 40°C, adjust the pH to 5, add compound enzyme 1, stir and hydrolyze at a rate of 40 rpm for 24 h to obtain an enzymolysis solution; wherein the mass ratio of alfalfa powder, pine needle powder, distilled water and compound enzyme 1 is 3:1:10:0.2; the compound enzyme 1 is trypsin and cellulase in a mass ratio of 3:0.8; concentrate the enzymolysis solution to a moisture content of 10% by rotary evaporation to obtain an alfalfa pine needle hydrolysate.

[0143] Comparative Example 4

[0144] Comparative Example 4 is the same as Example 1, except that the Maillard reaction product is not sprayed on the particle surface by vacuum spraying, but is mixed with other raw materials to obtain a mixed material.

[0145] A method for preparing a pet food that is intestinal-strengthening and nutritionally balanced for pets is as follows:

[0146] S1. Grind meat, vegetables and fruits respectively and pass them through a 40-mesh sieve to obtain meat puree, vegetable puree and fruit puree;

[0147] S2, placing meat puree, vegetable puree, fruit puree, whole goat milk powder, composite algae powder, yucca powder, synbiotic microcapsules, and Maillard reaction products in a blender, and stirring and mixing at a speed of 200 rpm for 3 hours to obtain a mixed material;

[0148] S3, placing the mixed material in an extruder to form cylindrical particles with a length of 3 cm and a diameter of 1 cm; wherein the extruder speed is 120 rpm and the extrusion temperature is 140°C;

[0149] S4, sending the cylindrical particles into a freeze-drying chamber, performing negative pressure freeze-drying at -30°C for 20 hours, until the moisture content is reduced to 4%, and taking out the freeze-dried particles;

[0150] S5. Pack the freeze-dried particles into bags, and vacuum and fill with nitrogen when packaging the products, so as to finally obtain pet food that is intestinal-friendly and nutritionally balanced.

[0151] Comparative Example 5

[0152] Comparative Example 5 is the same as Example 1, except that the composite algae powder is prepared only from Haematococcus pluvialis and Chlorella pyrenoidosa, as follows:

[0153] The preparation method of the composite algae powder is as follows:

[0154] B1. Mixing Haematococcus pluvialis and Chlorella pyrenoidosa and placing them in distilled water for ultrasonic cleaning at a frequency of 20 kHz to remove impurities, washing them with distilled water for 3 times after the ultrasonic cleaning, and drying them in an oven at 50° C. to obtain an algae mixture; wherein the mass ratio of Haematococcus pluvialis, Chlorella pyrenoidosa and distilled water is 2:1:20;

[0155] B2, crushing the algae mixture in a grinder, and passing through an 80-mesh sieve to obtain algae powder 1;

[0156] B3. Place algae powder 1, distilled water and compound enzyme 2 in a reaction container, adjust the pH to 5, and perform enzymolysis at 40 rpm at 40°C for 2 hours, then filter. Take the filter cake, wash it with distilled water 3 times, and dry it in an oven at 50°C to obtain algae powder 2; wherein the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:12:0.06, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 2:1.

[0157] B4. Place algae powder 2 in 100°C high-temperature steam sterilization for 2 hours, cool to room temperature, and obtain the desired composite algae powder.

[0158] Comparative Example 6

[0159] Comparative Example 6 is the same as Example 1, except that the composite algae powder is prepared only from Spirulina and Chlorella pyrenoidosa, as follows:

[0160] The preparation method of the composite algae powder is as follows:

[0161] B1. Mixing Spirulina and Chlorella vulgaris and placing them in distilled water for ultrasonic cleaning at a frequency of 20 kHz to remove impurities, washing them with distilled water for 3 times after the ultrasonic cleaning, and drying them in an oven at 50° C. to obtain an algae mixture; wherein the mass ratio of Spirulina, Chlorella vulgaris and distilled water is 5:1:20;

[0162] B2, crushing the algae mixture in a grinder, and passing through an 80-mesh sieve to obtain algae powder 1;

[0163] B3. Place algae powder 1, distilled water and compound enzyme 2 in a reaction container, adjust the pH to 5, and perform enzymolysis at 40 rpm at 40°C for 2 hours, then filter. Take the filter cake, wash it with distilled water 3 times, and dry it in an oven at 50°C to obtain algae powder 2; wherein the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:12:0.06, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 2:1.

[0164] B4. Place algae powder 2 in 100°C high-temperature steam sterilization for 2 hours, cool to room temperature, and obtain the desired composite algae powder.

[0165] Comparative Example 7

[0166] Comparative Example 7 is the same as Example 1, except that the composite algae powder is prepared only from Spirulina and Haematococcus pluvialis, as follows:

[0167] The preparation method of the composite algae powder is as follows:

[0168] B1. Mixing Spirulina and Haematococcus pluvialis, placing the mixture in distilled water and ultrasonically cleaning at a frequency of 20 kHz to remove impurities, washing with distilled water for 3 times after the ultrasonic cleaning, and drying in an oven at 50° C. to obtain an algae mixture; wherein the mass ratio of Spirulina, Haematococcus pluvialis, and distilled water is 5:2:20;

[0169] B2, crushing the algae mixture in a grinder, and passing through an 80-mesh sieve to obtain algae powder 1;

[0170] B3. Place algae powder 1, distilled water and compound enzyme 2 in a reaction container, adjust the pH to 5, and perform enzymolysis at 40 rpm at 40°C for 2 hours, then filter. Take the filter cake, wash it with distilled water 3 times, and dry it in an oven at 50°C to obtain algae powder 2; wherein the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:12:0.06, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 2:1.

[0171] B4. Place algae powder 2 in 100°C high-temperature steam sterilization for 2 hours, cool to room temperature, and obtain the desired composite algae powder.

[0172] Comparative Example 8

[0173] Comparative Example 8 is the same as Example 1, except that no compound enzyme 2 is added for enzymatic hydrolysis during the preparation of the composite algae powder, as follows:

[0174] The preparation method of the composite algae powder is as follows:

[0175] B1. Mixing Spirulina, Haematococcus pluvialis and Chlorella pyrenoidosa, placing the mixture in distilled water and ultrasonically cleaning at a frequency of 20 kHz to remove impurities, washing with distilled water for 3 times after the ultrasonic cleaning, and drying in an oven at 50° C. to obtain an algae mixture; wherein the mass ratio of Spirulina, Haematococcus pluvialis, Chlorella pyrenoidosa and distilled water is 5:2:1:20;

[0176] B2, crushing the algae mixture in a grinder, and passing through an 80-mesh sieve to obtain algae powder 1;

[0177] B3. Place the algae powder 1 in a 100°C high-temperature steam sterilizer for 2 hours, cool it to room temperature, and obtain the desired composite algae powder.

[0178] Comparative Example 9

[0179] Comparative Example 9 is the same as Example 1, except that, in the preparation process of the synbiotic microcapsules, the probiotic bacterial liquid is prepared only from Saccharomyces boulardii and Bacillus coagulans, as follows:

[0180] The preparation method of synbiotic microcapsules is as follows:

[0181] C1. Preparation of YPD liquid medium: weigh 20 g glucose, 20 g peptone, 10 g yeast powder, add distilled water to 1 L, adjust the pH to 7, and sterilize at 115°C for 20 min;

[0182] C2. Saccharomyces boulardii and Bacillus coagulans were cultured in YPD liquid culture medium respectively to obtain Saccharomyces boulardii culture liquid and Bacillus coagulans culture liquid, and the two were mixed to obtain probiotic culture liquid, wherein the number of live bacteria of Saccharomyces boulardii in the probiotic culture liquid was 1×10 8 CFU / g, the number of live Bacillus coagulans is 2×10 7 CFU / g;

[0183] C3. Mix the probiotic solution, oligofructose, 3% sodium carboxymethyl cellulose solution, and calcium carbonate to obtain a mixed solution 1; wherein the mass ratio of the probiotic solution, oligofructose, sodium carboxymethyl cellulose solution, and calcium carbonate is 1:5:10:0.8.

[0184] C4. Disperse the mixed solution 1 in soybean oil, stir and mix at a rate of 40 rpm for 0.2 h to form an oil-in-water emulsion, then add a citric acid solution with a mass concentration of 5%, stir and react at a rate of 40 rpm for 0.5 h, elute, and collect by centrifugation at 3000 rpm for 4 min to obtain the synbiotic microcapsules, wherein the mass ratio of the mixed solution 1, soybean oil and citric acid solution is 1:4:0.4.

[0185] Comparative Example 10

[0186] Comparative Example 10 is the same as Example 1, except that, in the preparation process of the synbiotic microcapsules, the probiotic bacterial liquid is prepared only from Lactobacillus mucosa and Bacillus coagulans, as follows:

[0187] The preparation method of synbiotic microcapsules is as follows:

[0188] C1. Preparation of YPD liquid medium: weigh 20 g glucose, 20 g peptone, 10 g yeast powder, add distilled water to 1 L, adjust the pH to 7, and sterilize at 115°C for 20 min;

[0189] C2. Lactobacillus mucosa and Bacillus coagulans were cultured in YPD liquid medium respectively to obtain Lactobacillus mucosa liquid and Bacillus coagulans liquid, which were mixed to obtain probiotic liquid, wherein the number of viable Lactobacillus mucosa in the probiotic liquid was 1×10 8 CFU / g, the number of live Bacillus coagulans is 2×10 7 CFU / g;

[0190] C3. Mix the probiotic solution, oligofructose, 3% sodium carboxymethyl cellulose solution, and calcium carbonate to obtain a mixed solution 1; wherein the mass ratio of the probiotic solution, oligofructose, sodium carboxymethyl cellulose solution, and calcium carbonate is 1:5:10:0.8.

[0191] C4. Disperse the mixed solution 1 in soybean oil, stir and mix at a rate of 40 rpm for 0.2 h to form an oil-in-water emulsion, then add a citric acid solution with a mass concentration of 5%, stir and react at a rate of 40 rpm for 0.5 h, elute, and collect by centrifugation at 3000 rpm for 4 min to obtain the synbiotic microcapsules, wherein the mass ratio of the mixed solution 1, soybean oil and citric acid solution is 1:4:0.4.

[0192] Comparative Example 11

[0193] Comparative Example 11 is the same as Example 1, except that, in the preparation process of the synbiotic microcapsules, the probiotic bacterial liquid is prepared only from Lactobacillus mucosa and Saccharomyces boulardii, as follows:

[0194] The preparation method of synbiotic microcapsules is as follows:

[0195] C1. Preparation of YPD liquid medium: weigh 20 g glucose, 20 g peptone, 10 g yeast powder, add distilled water to 1 L, adjust the pH to 7, and sterilize at 115°C for 20 min;

[0196] C2. Lactobacillus mucosa and Saccharomyces boulardii were cultured in YPD liquid medium respectively to obtain Lactobacillus mucosa liquid and Saccharomyces boulardii liquid, which were mixed to obtain probiotic liquid, wherein the number of viable Lactobacillus mucosa in the probiotic liquid was 1×10 8 CFU / g, the number of live bacteria of Saccharomyces boulardii is 1×10 8 CFU / g;

[0197] C3. Mix the probiotic solution, oligofructose, 3% sodium carboxymethyl cellulose solution, and calcium carbonate to obtain a mixed solution 1; wherein the mass ratio of the probiotic solution, oligofructose, sodium carboxymethyl cellulose solution, and calcium carbonate is 1:5:10:0.8.

[0198] C4. Disperse the mixed solution 1 in soybean oil, stir and mix at a rate of 40 rpm for 0.2 h to form an oil-in-water emulsion, then add a citric acid solution with a mass concentration of 5%, stir and react at a rate of 40 rpm for 0.5 h, elute, and collect by centrifugation at 3000 rpm for 4 min to obtain the synbiotic microcapsules, wherein the mass ratio of the mixed solution 1, soybean oil and citric acid solution is 1:4:0.4.

[0199] Comparative Example 12

[0200] Comparative Example 12 is the same as Example 1, except that the synbiotic microcapsules are replaced with synbiotics, as follows:

[0201] The preparation method of Synbiotics is as follows:

[0202] C1. Preparation of YPD liquid medium: weigh 20 g glucose, 20 g peptone, 10 g yeast powder, add distilled water to 1 L, adjust the pH to 7, and sterilize at 115°C for 20 min;

[0203] C2. Lactobacillus mucosa, Saccharomyces boulardii and Bacillus coagulans were cultured in YPD liquid medium respectively to obtain Lactobacillus mucosa liquid, Saccharomyces boulardii liquid and Bacillus coagulans liquid, which were mixed to obtain probiotic liquid, wherein the number of viable Lactobacillus mucosa in the probiotic liquid was 1×10 8 CFU / g, the number of live bacteria of Saccharomyces boulardii is 1×10 8 CFU / g, the number of live Bacillus coagulans is 2×10 7 CFU / g;

[0204] C3. Mixing the probiotic liquid and oligofructose to obtain the synbiotic, wherein the mass ratio of the probiotic liquid to the oligofructose is 1:5.

[0205] Performance Testing

[0206] The comprehensive performance of the pet intestinal-strengthening and nutritionally balanced pet food formulas prepared in Examples 1-3 of the present application and Comparative Examples 1-12 were tested respectively.

[0207] 1. Palatability test

[0208] During the test, 90 sterilized dogs of the same breed were randomly divided into 15 groups (each group included 3 female dogs and 3 male dogs, and the dogs selected for the experiment were all aged 1-3 years old)

[0209] Selective feeding rate test: Two feeding bowls were provided for the test dogs at the same time every day during the test period (9:30 a.m. and 4:30 p.m.), one of which was filled with the pet food of the test example (i.e., the pet food for strengthening the intestine and having balanced nutrition prepared in Examples 1-3 and Comparative Examples 1-12). The other feeding bowl was filled with commercially available pet freeze-dried food (Blue's squab freeze-dried dog food). The same amount was placed in each feeding bowl, and the feeding amount of each test dog on the two pet foods during one feeding process was weighed, and the average selective feeding rate of the group was calculated with a test period of 5 days, as well as the selectivity of the test dogs during one feeding process.

[0210] Feeding rate (%) = the amount of pet food consumed by the test dog during this feeding process / the total amount of food consumed by the test dog during this feeding process × 100%, the feeding bowls were swapped every other day. The results are shown in Table 1.

[0211] Table 1

[0212]

[0213]

[0214] It can be seen from the data shown in Table 1 that the comprehensive performance of the pet food prepared in Examples 1-3 is far better than that in Comparative Examples 1-12, and the pet food in Examples 1-3 has better palatability, and pets like to eat it more.

[0215] 2. Stool characteristics test

[0216] 90 Chinese rural cats, aged 6-12 months, with an average weight of (3.42±0.86) kg, were collected and randomly divided into 15 groups (Examples 1-3, Comparative Examples 1-12), with 6 Chinese rural cats in each group, and the ratio of male cats to female cats in each group was 1:1. Each group was fed with the pet food prepared in Examples 1-3 and Comparative Examples 1-12, respectively.

[0217] The fecal status of each group of cats was observed daily, and the fecal scores were performed according to the standards in Table 2. The diarrhea rate and soft stool rate were calculated based on the fecal scores. The calculation formula is as follows:

[0218] Diarrhea rate (%) = number of diarrhea occurrences per cat / total number of feces scoring statistics per cat × 100%; soft stool rate (%) = number of soft stool occurrences per cat / total number of feces scoring statistics per cat × 100%. The results are shown in Table 3.

[0219] Table 2

[0220]

[0221]

[0222] Note: 1≤stool score <2 indicates constipation, 2≤stool score ≤3 indicates normal, 3<stool score <4 indicates soft stool, and 4≤stool score ≤5 indicates diarrhea.

[0223] Table 3

[0224] Fecal scoring Diarrhea rate (%) Soft stool rate (%) Example 1 2.51 1.32 3.28 Example 2 2.50 1.08 3.09 Example 3 2.54 1.68 3.98 Comparative Example 1 2.64 4.56 8.61 Comparative Example 2 2.62 4.72 8.63 Comparative Example 3 2.69 4.83 8.67 Comparative Example 4 2.64 4.32 8.84 Comparative Example 5 2.95 8.7 17.3 Comparative Example 6 2.94 8.3 18.1 Comparative Example 7 2.89 7.9 15.9 Comparative Example 8 2.71 7.4 13.4 Comparative Example 9 2.98 9.1 17.6 Comparative Example 10 2.94 8.9 17.1 Comparative Example 11 2.95 9.2 17.4 Comparative Example 12 2.85 8.8 16.3

[0225] From the data shown in Table 3, it can be seen that the comprehensive performance of the pet food prepared in Examples 1-3 is far superior to that of Comparative Examples 1-12. After the pets eat the pet food prepared in Examples 1-3, the feces are healthy, the diarrhea rate and the soft stool rate are low, and the pets' intestines are healthy.

[0226] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A pet food formula for pets with intestinal health and balanced nutrition, characterized in that: The invention comprises the following raw materials in parts by weight: 60-80 parts of meat, 10-20 parts of vegetables, 5-10 parts of fruits, 2-4 parts of whole goat milk powder, 2-4 parts of Maillard reaction products, 2-4 parts of compound algae powder, 0.5-1.5 parts of yucca powder and 0.4-0.8 parts of synbiotic microcapsules.

2. The pet food formula for strengthening the pet intestine and having balanced nutrition according to claim 1, characterized in that: The preparation method of the Maillard reaction product is as follows: A1. Grind alfalfa and pine needles in a grinder respectively, and pass through an 80-100 mesh sieve to obtain alfalfa powder and pine needle powder; A2, placing alfalfa powder and pine needle powder in distilled water, adding compound enzyme 1 for enzymolysis to obtain an enzymolysis solution, and concentrating the enzymolysis solution by rotary evaporation to a water content of 10-15%, thereby obtaining an enzymolysis product; A3. Mix the enzymatic hydrolysate and reducing sugar, adjust the pH to 4-6, adjust the temperature to 80-100°C, react for 0.8-1.2h, filter and dry, and crush through a 80-120 mesh sieve to obtain the desired Maillard reaction product.

3. The pet food formula for strengthening the intestines and having balanced nutrition according to claim 2, characterized in that: In the step A2, the mass ratio of alfalfa powder, pine needle powder, distilled water and compound enzyme 1 is 3-5:1-2:10-14:0.2-0.6; the compound enzyme 1 is a mixture of trypsin and cellulase in a mass ratio of 3-5:0.8-1.

2.

4. The pet food formula for strengthening the pet intestine and having balanced nutrition according to claim 2, characterized in that: In the step A3, the mass ratio of the enzymatic hydrolysate to the reducing sugar is 3-5:0.2-0.6; the reducing sugar is at least one of glucose, fructose and galactose.

5. The pet food formula for strengthening the pet intestine and having balanced nutrition according to claim 1, characterized in that: The preparation method of the composite algae powder is as follows: B1. Mixing Spirulina, Haematococcus pluvialis and Chlorella pyrenoidosa, and then placing the mixture in distilled water for ultrasonic cleaning at a frequency of 20-40 kHz to remove impurities, and then washing the mixture with distilled water for 3-5 times after the ultrasonic cleaning, and drying the mixture in an oven at 50-60° C. to obtain an algae mixture; B2, crushing the algae mixture in a grinder, and passing through an 80-120 mesh sieve to obtain algae powder 1; B3, placing algae powder 1, distilled water and complex enzyme 2 in a reaction container, adjusting the pH to 5-7, stirring and enzymolyzing at 40-50° C. for 2-4 hours, and then filtering, taking the filter cake, washing it with distilled water for 3-5 times, and then drying it to obtain algae powder 2; B4. Place algae powder 2 in high temperature steam for sterilization for 2-3 hours, and cool to obtain the desired composite algae powder.

6. The pet food formula for strengthening the intestines and having balanced nutrition according to claim 5, characterized in that: In the step B1, the mass ratio of Spirulina, Haematococcus pluvialis, Chlorella pyrenoidosa and distilled water is 5-8:2-4:1-2:20-30; in the step B3, the mass ratio of algae powder 1, distilled water and compound enzyme 2 is 1:12-16:0.06-0.08, and the compound enzyme 2 is a mixture of cellulase and pectinase in a mass ratio of 2-3:1-2.

7. The pet food formula for strengthening the intestines and having balanced nutrition according to claim 1, characterized in that: The preparation method of the synbiotic microcapsule is as follows: C1. Culturing probiotics in a culture medium to obtain a probiotic solution; C2, mixing the probiotic solution, the prebiotic, a sodium carboxymethyl cellulose solution with a mass concentration of 3-5%, and insoluble calcium salt particles to obtain a mixed solution 1; C3, dispersing the mixed solution 1 in soybean oil, stirring and mixing to form a water-in-oil emulsion, then adding a citric acid solution with a mass concentration of 5-10%, stirring and reacting for 0.5-1h, eluting, and collecting by centrifugation to obtain the synbiotic microcapsules.

8. The pet food formula for strengthening the intestines and having balanced nutrition according to claim 7, characterized in that: In step C1, the probiotics are composed of Lactobacillus mucosa, Saccharomyces boulardii and Bacillus coagulans, and the number of viable Lactobacillus mucosa in the probiotic solution is ≥ 1×10 8 CFU / g, the number of live bacteria of Saccharomyces boulardii ≥ 1×10 8 CFU / g, the number of live Bacillus coagulans ≥ 2×10 7 CFU / g; the prebiotic in step C2 is at least one of oligofructose, oligogalactose and inulin; and the insoluble calcium salt particles are calcium carbonate or calcium phosphate.

9. The pet food formula for strengthening the pet intestine and having balanced nutrition according to claim 7, characterized in that: The mass ratio of the probiotic solution, prebiotics, sodium carboxymethyl cellulose solution and insoluble calcium salt particles in step C2 is 1:5-7:10-14:0.8-1.2; the mass ratio of the mixed solution 1, soybean oil and citric acid solution in step C3 is 1:4-6:0.4-0.

8.

10. A method for preparing the intestinal-strengthening and nutritionally balanced pet food according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1, crushing meat, vegetables and fruits respectively to obtain meat puree, vegetable puree and fruit puree; S2, placing meat puree, vegetable puree, fruit puree, whole goat milk powder, compound algae powder, yucca powder, and synbiotics microcapsules in a blender, stirring and mixing to obtain a mixed material; S3, placing the mixed material in an extruder to form cylindrical particles with a length of 3-5 cm and a diameter of 1-2 cm; S4, using vacuum spraying equipment to spray the Maillard reaction product on the particle surface; S5. After spraying, the cylindrical particles are sent to a freeze-drying chamber and subjected to negative pressure freeze drying at -30--50°C for 20-30 hours until the moisture content is reduced to 4-6%, and the freeze-dried particles are taken out; S6. Pack the freeze-dried particles into bags, and vacuum and fill with nitrogen when packaging the products, so as to finally obtain pet food that is intestinal-friendly and nutritionally balanced.

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