Microorganism real-time tracking detection and drug sensitivity analysis system

Abstract

The invention provides a microorganism real-time tracking detection and drug sensitivity analysis system. The system comprises a transmission module, a temperature control module, a light path module, a detection module, a shell module and an XY slide rail module. Wherein the transmission module comprises a driving motor, a motor driving plate, a sliding rail, a belt rod frame, a belt clamp and a belt adjuster; the temperature control module has heating and refrigerating functions and comprises a heating film, two temperature sensors, a fan, a refrigerating piece, a copper pipe, a water pump, a radiator and a fixing assembly. The light path module comprises an LED, a filter wheel, an LED lens, an LED support, an LED drive board, a lampshade, a cooling fin, a fan and an optical fiber. The detection module comprises a photodiode receiving plate, a mainboard PCB, a clamping plate, a clamping plate cover and a bracket; the shell module comprises a bottom shell, a shell, a foot pad, a rear cover and a rear locking piece; the XY sliding rail module comprises an XY moving shaft, a driving motor, a crawler belt and an infrared sensor. The microorganism real-time detection system has good microorganism detection sensitivity and accuracy, realizes high-throughput growth curve measurement, simplifies operation of experimenters, shortens detection time and improves experiment efficiency and determination accuracy.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection, and in particular to a microbial real-time tracking detection and drug sensitivity analysis system. Background Art

[0002] Microbial detection is a key technology that is widely used in medicine, environmental science, food safety, and pharmaceuticals. Its main purpose is to detect and quantify the presence of microorganisms in samples, including bacteria, fungi, viruses and other microorganisms. This technology has important applications in different fields and provides key support for ensuring public health, food safety, and production quality in the pharmaceutical industry. Traditional microbial detection methods have problems such as long culture time and unclear characteristics, and the real-time detection is not good; the Mérieux drug sensitivity analysis system is expensive and not suitable for ordinary corporate users; the common test strip drug sensitivity analysis has a relatively low throughput and cumbersome testing. In view of this, the present invention is specially proposed. Summary of the invention

[0003] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a microbial real-time tracking detection and drug sensitivity analysis system.

[0004] The objective of the present invention is achieved through the following technical solutions: A microbial real-time tracking detection and drug sensitivity analysis system includes: a transmission module, a temperature control module, an optical path module, a detection module, and a shell module. Among them, the transmission module includes a drive motor, a motor drive board, a slide rail, a belt rod frame, a belt clamp, and a belt adjuster; the temperature control module has heating and cooling functions, including a heating film, two temperature sensors, a fan, a cooling plate, a copper pipe, a water pump, a radiator and a fixing component; the optical path module includes an LED, a filter wheel, an LED lens, an LED bracket, an LED drive board, a lampshade, a heat sink, a fan, and an optical fiber; the detection module includes a photodiode receiving board, a mainboard PCB, a card board, a card board cover, and a bracket; the shell module includes a bottom shell, a shell, a foot pad, a back cover, and a back lock; the XY slide rail module includes an XY moving axis, a drive motor, a crawler, and an infrared sensor.

[0005] In one embodiment, the housing module includes an outer shell, a bottom shell, a back cover and a foot pad, which are fixed to each other with screws to carry the real-time microbial detection system.

[0006] In one of the embodiments, the functions of the temperature control module are implemented using embedded software code, specifically including heating film control, cooling plate control, temperature reading, temperature control and fan control. The module is fixed on the bottom shell and the slide rail to directly control the temperature of the 96-well plate of the microbial incubator to keep the equipment temperature within the set range.

[0007] In one embodiment, the functions of the transmission module are implemented using embedded software code, specifically including motor operation and oscillation control, controlling the stepper motor to rotate the belt to make the 96-well plate reach the sampling position, controlling the stepper motor to rotate forward and reverse to achieve oscillation, and the motor automatically returns to the origin after power-on.

[0008] In one of the embodiments, the functions of the optical path module and the detection module are implemented using embedded code, specifically including LED light source on and off control, data acquisition and serial port communication. During the detection process, the LED light source is controlled to emit light, OD detection is performed on each channel through the LED lens and optical fiber, the photodiode collects the signal strength, and the value is sent to the host computer for display in the interface.

[0009] In one of the embodiments, the workflow of the microbial real-time system is as follows: the single-chip microcomputer receives the initialization instruction, controls the transmission module to move the 96-well plate to the detection culture chamber, determines whether the temperature reaches the set condition, and after reaching the set temperature, the motor oscillates the reagent. After being stationary for a set time, the transmission module moves the 96-well plate to the sampling point, collects data and sends the results to the host computer, repeats the collection steps to complete the collection of all channels, and collects again at the next collection time interval.

[0010] Compared with the prior art, the present invention has at least the following advantages: In the real-time microbial detection system of the present invention, a stepper motor is used to control the oscillation of a 96-well plate, a temperature control module controls the microbial culture temperature, and a light source and a detection module detect multi-channel photoelectric signals, so the system has the functions of microbial culture and real-time detection; the photoelectric detection adopts a filter wheel to select the wavelength, which expands the application range of the system; the detection is realized by using an XY-axis moving detection table, so that the system can be applied to microplate detection of different specifications; the upper and lower temperature sensors simultaneously measure and control the temperature, effectively preventing the occurrence of water mist interfering with the detection, and improving the accuracy of microbial detection; the detection of high-throughput microbial growth curves simplifies the operation of the experiment, reduces the labor intensity of the experimenters, and improves the experimental efficiency and the accuracy of microbial detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0012] Figure 1 Schematic diagram of the housing structure of an embodiment of the present invention; Figure 2 It is a schematic diagram of the structure of an incubator according to an embodiment of the present invention; Figure 3 A schematic diagram of a receiving module according to an embodiment of the present invention; Figure 4 Schematic diagram of a detection module according to an embodiment of the present invention; Figure 5 A schematic diagram of an optical path module according to an embodiment of the present invention; Figure 6 Schematic diagram of an XY slide rail module according to an embodiment of the present invention; Figure 7 Schematic diagram of a temperature control module according to an embodiment of the present invention; Figure 8 Schematic diagram of the detection optical path of an embodiment of the present invention; Fig. 9 Schematic diagram of the detection principle of an embodiment of the present invention; Fig.10 A schematic diagram of the control principle of an embodiment of the present invention; Fig.11 This is an I / V conversion circuit diagram of an optoelectronic module according to an embodiment of the present invention; Fig.12 MOS tube driving circuit diagram of an embodiment of the present invention; Fig.13 A full-bridge driving circuit diagram of an embodiment of the present invention; Fig.14 A schematic diagram of a motor drive according to an embodiment of the present invention; Fig.15 A schematic diagram of data transmission hardware according to an embodiment of the present invention; Fig.16 It is a schematic diagram of the overall architecture of the embedded software according to the embodiment of the present invention; Fig.17 It is a flow chart of temperature control implementation in an embodiment of the present invention; Fig.18 A communication flow chart of an embodiment of the present invention; Fig.19 The OD-time curve diagram of the drug sensitivity test of carbenicillin on Escherichia coli using the real-time microbial detection system according to the embodiment of the present invention; Fig. 20 The OD-time curve diagram of the drug sensitivity test of gentamicin on Staphylococcus aureus using the real-time microbial detection system according to the embodiment of the present invention; In the figure: 1. power switch; 2. rear cover; 3. detection component; 4. rear lock; 5. shell; 6. LED lock; 7. foot pad; 8. bottom shell; 9. power input board PCB; 10. receiving board assembly; 11. heating film; 12. upper temperature sensor; 13. main board PCB; 14. detection module; 15. bottom plate; 16. lower temperature sensor; 17. drive motor; 18. belt rod rack; 19. slide rail; 20. U-shaped rack; 21. temperature control module; 22. optical path module; 23. driven wheel reinforcement; 24. optical fiber fixing head; 25. driven wheel assembly; 26. U-shaped fixing plate; 27. water pump; 28. sensor; 29. ​​flip cover; 30. receiving board; 31. heating film; 32. upper temperature sensor; 33. main board PCB; 34. photoelectric fixing rack; 35 , slide rail; 36, copper tube; 37, bracket; 38, pallet; 39, pallet cover; 40, copper tube cover; 41, belt adjuster; 42, belt clamp; 43, heat sink; 44, LED; 45, filter; 46, filter wheel; 47, lampshade; 48, drive motor; 49, LED bracket; 50, optical fiber; 51, XY moving axis; 52, drive motor; 53, crawler; 54, slide bar; 55, detection base plate; 56, detection PCB; 57, lens; 58, reflector; 59, optical fiber; 60, upper cover; 61, detection table; 62, lower cover; 63, slider connecting frame; 64, crawler; 65, slider; 66, slide bar; 67, infrared sensor; 68, fan; 69, radiator; 70, cooling plate; 71, cooling plate bracket; 73, receiving end convex lens. DETAILED DESCRIPTION

[0013] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. The preferred embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thoroughly understood.

[0014] Example 1: A real-time tracking, detection and analysis system for microorganisms The present application provides a microbial real-time tracking detection and drug sensitivity analysis system. The above-mentioned microbial real-time detection system includes a housing structure and a 96-well plate, and detects 8 channels each time. Under working conditions, variables such as the temperature of each channel do not change much, which effectively improves the sensitivity of microbial detection.

[0015] See also Figure 1In one embodiment, a housing support structure of a microbial real-time tracking detection and drug sensitivity analysis system includes a power switch 1, a rear cover 2, a detection component 3, a rear lock 4, a housing 5, an LED lock 6, a foot pad 7, a bottom shell 8 and a power input board PCB 9. Specifically, the power switch 1 is stuck on the rear cover 2, the rear cover 2 and the rear lock 4 are fixed with screws, and the housing 5 and the detection component 3, the LED lock key 6, the foot pad 7, the bottom shell 8 and the power input board PCB 9 are fixed with screws. The housing shell completely covers the system incubator, provides support in a microbial real-time tracking detection and drug sensitivity analysis system, and eliminates external interference.

[0016] See also Figure 2 In one embodiment, a culture box structure of a microbial real-time tracking detection and drug sensitivity analysis system includes a receiving plate assembly 10, a heating film 11, an upper temperature sensor 12, a main board PCB 13, a detection module 14, a bottom plate 15, a lower temperature sensor 16, a drive motor 17, a belt rod rack 18, a slide rail 19, a U-shaped rack 20, a temperature control module 21, an optical path module 22, a driven wheel reinforcement 23, an optical fiber fixing head 24, a driven wheel assembly 25, a U-shaped fixing plate 26, a water pump 27, a sensor 28 and a flip cover 29.

[0017] Specifically, the U-shaped frame 20 and the detection module 14, the temperature control module 21, the optical path module 22, the optical fiber fixing head 24, the water pump 27, and the flip cover 29 are fixed with screws. The bottom plate 15 and the drive motor 17 and the sensor 28 are fixed with screws. The driven wheel assembly 25 is fixed on the belt rod frame 18, and the driven wheel reinforcement 23 and the receiving plate assembly 10 are respectively fixed on the belt rod frame 18 and the U-shaped frame 20 with screws.

[0018] Furthermore, an incubator for a microbial real-time tracking detection and drug sensitivity analysis system has the functions of constant temperature cultivation of microorganisms, oscillation and detection. The main board PCB13 controls the power adjustment temperature of the heating film 11, the temperature control module 21 and the water pump 27 through the temperature feedback of the upper temperature sensor 12 and the lower temperature sensor 16; the oscillation and detection positioning of the 96-well plate are controlled by the driving motor 17, the belt rod rack 18, the slide rail 19, the driven wheel reinforcement 23, the driven wheel assembly 25 and the sensor 28; the signal of each channel is detected by the receiving board assembly 10, the detection module 14, the optical path module 22 and the optical fiber fixing head 24.

[0019] See also Figure 3 In one embodiment, a receiving module of a microbial real-time tracking detection and drug sensitivity analysis system includes a heating film 31, an upper temperature sensor 32, and a main board PCB 33.

[0020] See also Figure 4In one embodiment, a detection module of a microbial real-time tracking detection and drug sensitivity analysis system includes a slide rail 35, a copper tube 36, a bracket 37, a card board 38, a card board cover 39, a copper tube cover 40, a belt adjuster 41 and a belt clip 42.

[0021] Specifically, the bracket 37 and the slide rail 35, the copper tube 36, the clamping plate 38, the belt adjuster 41 and the belt clamp 42 are fixed with screws.

[0022] Furthermore, the 96-well plate is placed on the bracket 37, and the 96-well plate is moved back and forth on the slide rail 35 by controlling the stepper motor to rotate and drag the belt, thereby achieving oscillation of the sample.

[0023] See also Figure 5 In one embodiment, an optical path module of a microorganism real-time tracking detection and drug sensitivity analysis system includes a heat sink 43, an LED 44, an LED lens 45, a filter wheel 46, a lampshade 47, a drive motor 48, an LED bracket 49 and an optical fiber 50.

[0024] Specifically, LED 44 is welded on the aluminum substrate of the lamp bead, and a heat sink 43 is attached to dissipate heat of the lamp bead. The LED lens 45 is fixed on the filter wheel 46. The drive motor 48 can be used to rotate and select lenses of different wavelengths for detecting microorganisms of different wavelengths. The LED 44 and the LED lens 45 are fixed at the same height and level with the light source to ensure that the filter wheel rotates and selects the same position, so that the light source is emitted into the optical fiber 50 to ensure that the light intensity of the detection channel is consistent. The lampshade 47 ensures that external light will not affect the LED light source. The drive motor 48 is fixed on the lampshade 47 to improve the reliability of the system.

[0025] See also Figure 6 In one embodiment, an XY slide rail module of a microorganism real-time tracking detection and drug sensitivity analysis system includes an XY moving axis 51, a driving motor 52, a track 53, a slide bar 54, a detection base plate 55, a detection PCB 56, a lens 57, a reflector 58, an upper cover 60, a detection table 61, a lower cover 62, a slider connecting frame 63, a track 64, a slider 65, a slide bar 66 and an infrared sensor 67.

[0026] Specifically, the driving motor 52 controls the detection platform 61 to move on the slide bar 54 through the crawler 53 to perform multi-channel detection. An infrared sensor 67 is fixed on the XY moving axis to confirm the position of the slide; the detection base plate 55, the detection PCB56, the lens 57, the reflector 58, the optical fiber 59, the upper cover 60 and the lower cover 62 are integrated and fixed, the optical fiber 59 and the optical fiber 50 are the same optical fiber, and the light source of the detection platform 61 is the light source transmitted by the LED lens 45 in the filter light wheel 46 of the optical path module LED44 of the microorganism real-time detection system. The LED lens with a suitable wavelength is selected according to the type of microorganism to be detected; the slide connecting frame 63 and the slide 65 are fixed, and the whole is moved horizontally by the slide bar for multi-channel detection; the detection signal result is transmitted from the detection board to the main board PCB33 to draw the microorganism growth curve.

[0027] See also Figure 7 In one embodiment, a temperature control module of a microbial real-time tracking detection and drug sensitivity analysis system includes a fan 68, a radiator 69, a cooling plate 70 and a cooling plate bracket 71.

[0028] Specifically, the cooling fin 70 and the cooling fin bracket 71 are fixed to the radiator 69 by screws, the fan 68 is fixed to the radiator 69 by screws, and the temperature control module is used for cooling and constant temperature control.

[0029] like Figure 8 As shown, a detection optical path of a microorganism real-time tracking detection and drug sensitivity analysis system, LED 44 generates a light signal through filter 45 to generate monochromatic light, and the monochromatic light is transmitted to one end of reflector 58 through optical fiber 59. The monochromatic light is reflected by reflector 58 to lens 57 and converged into microorganism sample 72, and the monochromatic light signal passing through microorganism sample 72 is then converged into detection PCB 56 through receiving end convex lens 73 for detecting changes in light signals.

[0030] like Fig. 9 As shown, the detection principle of a microbial real-time tracking detection and drug sensitivity analysis system is similar to the Lambert-Beer law. First, the AD converted by the detection PCB56 at time T is recorded at 0. 0， In the following moments, the data AD will be detected in real time t By the formula OD = -log 10 (AD t / AD0) is converted into OD value for curve output display. As time goes by, the growth of microorganisms causes the microorganism sample 72 to become turbid, and the monochromatic light signal received by the detection PCB 56 decreases, resulting in AD t Decreases, thereby increasing OD, and the output curve is similar to an "S"-shaped curve.

[0031] like Fig.10As shown in the figure, a control principle of a microbial real-time tracking detection and drug sensitivity analysis system is that an external power supply supplies power to each module of the system, and the transmission module, temperature control module, light source module and detection module are controlled by a microcontroller. The control circuit of the transmission module controls the step length and speed of the drive motor, and feeds back the sample position indicated by the infrared sensor to the microcontroller; the control circuit of the light source module controls the operating power of the LED; the control circuit of the detection module feeds back the collected data after photoelectric conversion to the microcontroller; the control circuit of the temperature control module controls the operating power of the heating film, water pump, cooling plate and fan, and feeds back the temperature collected data of the temperature sensor to the microcontroller.

[0032] like Fig.11 As shown, the detection module collects photoelectric data through an I / V conversion circuit. The photodiode D13 receives the light signal and generates a microampere current. A 200k ohm resistor R41 is used to convert the current signal into a voltage signal. The output D_CH8 of the entire I / V conversion circuit is received by the ADC pin of the microcontroller and converted into an AD value by the microcontroller.

[0033] like Fig.12 As shown, the control of the heating film 31, the water pump 27, the fan 68, the LED 44 and the fan at the light source end is realized by the MOS driving circuit. Taking the control circuit of the water pump as an example, an optocoupler U10 is used to isolate the microcontroller circuit part from the water pump driving circuit. The water pump is connected to the VCC_24V voltage through a PMOS tube Q5, and the G pole of Q5 is connected to GND through an NMOS tube Q6. Therefore, when the output PUMB_CNT of the microcontroller is high, the water pump is connected to VCC_24V and the water pump starts to run.

[0034] like Fig.13 As shown, the control of the cooling sheet 70 is realized by a full-bridge circuit, and the full-bridge driving circuit is composed of 4 NMOS tubes. The output signals of the microcontroller are PTC_IN1 and PTC_IN2, and two IR2104STRPBF chips are used to control the conduction and cutoff of the four MOS tubes. When PTC_IN1 is high and PTC_IN2 is low, the HO and LO pins of U9 are high and low respectively, and the HO and LO pins of U36 are opposite to them, which makes Q7 and Q10 conduct. At this time, the current is a forward current, and the cooling sheet 70 realizes the heating function, otherwise it realizes the cooling function. If PTC_IN1 and PTC_IN2 are both low, the cooling sheet 70 does not work.

[0035] like Fig.14As shown, the control of the drive motor 17, the drive motor 48, and the drive motor 52 is jointly implemented by the microcontroller and the motor driver chip TMC2660-PA. The microcontroller is connected to the TMC2660-PA via the SPI interface to implement the initialization configuration of the TMC2660-PA. At the same time, a TIMER pin of the microcontroller is used to connect the STEP pin of the TMC2660-PA, and a pulse signal is output through the TIMER pin for step control. A GPIO pin of the microcontroller is used to connect the DIR pin of the TMC2660-PA for direction control. The OA1, OA2, OB1, and OB2 pins of the TMC2660-PA chip are connected to the stepper motor. Among them, the OA1 and OA2 pins are used to control the phase of the stepper motor and adjust the rotation direction and speed of the motor. The OB1 and OB2 pins are used to set the drive current of the stepper motor to ensure that the motor obtains appropriate driving force under various load conditions.

[0036] like Fig.15 As shown in the figure, the microcontroller needs to send the system's detection data to the computer for curve display. The TTL level standard commonly used in the circuit cannot be directly recognized by the microcontroller as the RS232 level. Therefore, an SP232 chip is used to achieve communication level matching between the microcontroller and the computer. Among them, the USART_TX and USART_RX pins of the microcontroller are connected to the SP232 chip, and the level converted by SP232 is output to the standard DB9 interface. The communication connection between the microcontroller and the computer can be achieved through a DB9 to USB data cable.

[0037] like Fig.16 As shown in the figure, an embedded software architecture of a microbial real-time tracking detection and drug sensitivity analysis system is divided into a driver layer, an intermediate layer and an application layer. The driver layer initializes the GPIO, TIMER, USART, EXTI and SPI related pins and their functions of the microcontroller. The intermediate layer is the operating system layer, and the system uses the FreeRTOS real-time operating system as the intermediate layer. The application layer performs photoelectric acquisition, motor drive control, temperature control and data transceiver functions.

[0038] The temperature control of the system is as follows Fig.17 As shown in the figure, first set the temperature value T0, and perform PID operation according to the feedback value T of the temperature sensor. When the PID output is greater than 0, the cooling plate and the heating film are controlled to increase the temperature; when the PID output is less than 0, the cooling plate is controlled to cool down and the heating film is stopped; in other cases, the cooling plate and the heating film are stopped to maintain the current temperature.

[0039] A communication protocol between a microbial real-time tracking detection and drug sensitivity analysis system and a computer Fig.18As shown, when the first data bit after the frame header of the data frame sent by the computer contains data of 0x01, the microcontroller parses out the basic configuration information, such as setting the temperature T0, and starts the microbial tracking detection. After that, the microcontroller continuously sends the data frame with the first data bit after the frame header containing data of 0x30 to the computer, and the computer parses out the microbial tracking detection data of the system. At the same time, the microcontroller continuously sends the data frame with the first data bit after the frame header containing data of 0x40 to the computer, and the computer parses out the real-time reading temperature T of the system. When the first data bit after the frame header of the data frame sent by the computer contains data of 0x02, the microcontroller receives the data and controls the system to stop detection.

[0040] In one of the embodiments, the parameters that can be set include light source wavelength, growth temperature, vibration duration, vibration frequency, detection duration, and detection interval duration; the parameters displayed in real time include experimenter, experiment number, real-time temperature, operating time, instrument status, and real-time curve.

[0041] In one embodiment, the real-time curve results of the detection are automatically saved after the experiment is completed, and can be selected for viewing.

[0042] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims. Embodiment 2

[0043] Take the drug sensitivity study of Escherichia coli to carbenicillin as an example. (The configuration of each unit component of the system is the same as that of Example 1) The parameters were set as follows: light source wavelength 600nm, growth temperature 37℃, vibration duration 10s, vibration frequency 25Hz, detection duration 18h, and detection interval 3min; Fig.11 The OD curve obtained from the drug sensitivity test of Escherichia coli to carbenicillin is shown. In this experiment, the concentrations of carbenicillin were 0, 4, 8, 12, 16, 20 and 24 ug / mL, and the initial concentration of Escherichia coli was 10 6 CFU / mL. Fig.11In the experiment, the growth curve of the positive control sample (containing bacteria but no antibiotics) was almost S-shaped. The time for E. coli exposed to 8 ug / mL of carbenicillin to reach the inflection point was significantly longer than that for E. coli exposed to 4 ug / mL of carbenicillin, indicating that changes in antibiotic concentration can affect the inflection point of microbial growth. Compared with the positive control sample, the OD value of the E. coli sample decreased with the increase of carbenicillin concentration. When the carbenicillin concentration reached 16 ug / ml, the growth of E. coli was completely inhibited. According to the definition of minimum inhibitory concentration (MIC), the MIC value of E. coli susceptibility study to carbenicillin was 16 ug / mL. Embodiment 3

[0044] Take the drug sensitivity study of Staphylococcus aureus to gentamicin as an example. (The configuration of each unit component of the system is the same as that of Example 1) The parameters were set as follows: light source wavelength 600nm, growth temperature 37℃, vibration duration 10s, vibration frequency 25Hz, detection duration 18h, and detection interval 3min; Fig.12 The OD curve obtained from the drug sensitivity test of Staphylococcus aureus to gentamicin is shown. In this experiment, the concentrations of gentamicin were 0, 3, 6, 9, 12, 15 and 18 ug / mL, and the initial concentration of Staphylococcus aureus was 10 6 CFU / mL. Fig.12 In the embodiment, similar to Example 2, the growth curve of the positive control sample is almost S-shaped, and the time when the Staphylococcus aureus exposed to 9ug / mL gentamicin reaches the inflection point is significantly greater than the time when the Staphylococcus aureus exposed to 6 ug / mL gentamicin reaches the inflection point. Compared with the positive control sample, as the concentration of gentamicin increases, the OD value of the Staphylococcus aureus sample decreases. When the concentration of gentamicin reaches 15ug / ml, the growth of Staphylococcus aureus is completely inhibited, and the MIC value of the drug sensitivity study of Staphylococcus aureus to Staphylococcus aureus is 15ug / mL. However, in the time period of about 760min to 1080min, the OD value obtained by the growth of Staphylococcus aureus exposed to 9 ug / mL gentamicin is slightly higher than the OD value obtained by the growth of Staphylococcus aureus exposed to 6 ug / mL gentamicin, resulting in an abnormal phenomenon that the inhibitory effect of the antibiotic concentration increases but decreases. This shows that over time, some Staphylococcus aureus have developed resistance to gentamicin during the culture process, which has led to the appearance of this abnormal phenomenon.

Claims

1. A microbial real-time tracking detection and drug sensitivity analysis system, characterized in that: include: Transmission module, temperature control module, optical module, detection module, and housing module. The transmission module includes a drive motor, a motor drive board, a slide rail, a belt rod rack, a belt clamp, and a belt adjuster; the temperature control module has heating and cooling functions, including a heating film, two temperature sensors, a fan, a cooling plate, a copper tube, a water pump, a radiator, and a fixing component; the optical module includes an LED, a filter wheel, an LED lens, an LED bracket, an LED drive board, a lampshade, a heat sink, a fan, and an optical fiber; the detection module includes a photodiode receiving board, a mainboard PCB, a card board, a card board cover, and a bracket; the housing module includes a bottom shell, a housing, a foot pad, a rear cover, and a rear lock; the XY slide rail module includes an XY moving axis, a drive motor, a crawler, and an infrared sensor.

2. A microbial real-time tracking detection and drug sensitivity analysis system according to claim 1, characterized in that: The transmission module transmits the control signal from the main board to the motor drive board and drives the motor shaft to rotate. The shaft and the driven wheel shaft control the movement of the 96-well plate on the slide rail through the belt and the belt rod frame, and the control signal output realizes the oscillation of the 96-well plate.

3. A microbial real-time tracking detection and drug sensitivity analysis system according to claim 1, characterized in that: The temperature control module uses a heating film as a heating source, uses a lower temperature sensor to monitor the microbial culture temperature, uses air cooling and water cooling for cooling, and the temperature control range is 25-60°C. In the culture process, the temperature of each channel of the 96-well plate is controlled at 37±0.3°C, and the temperature of the upper temperature sensor is controlled to be greater than the temperature of the lower temperature sensor to ensure that no water mist is generated on the top cover.

4. A microbial real-time tracking detection and drug sensitivity analysis system according to claim 1, characterized in that: The LED light source power in the optical path module is 3W, and the heat is dissipated by an aluminum substrate and a fan to ensure the consistency and stability of the light source intensity during the detection process.

5. A microbial real-time tracking detection and drug sensitivity analysis system according to claim 1, characterized in that: A plurality of LED lenses in the optical path module are fixed on the filter wheel, and the LED lenses can be selected to filter the light source emitted by the LED and then emit it into the optical fiber.

6. A microbial real-time tracking detection and drug sensitivity analysis system according to claim 1, characterized in that: The detection module is placed on the slide rail, a 96-well plate is placed on the bracket, and there are 8 holes and grooves at the bottom to fix 8 optical fibers. The belt is driven by the shaft to move the 96-well plate and the bracket to achieve high-throughput detection. The photodiode is aligned with the optical fiber outlet and fixed to the upper end of the heating film at a certain distance with a photoelectric fixing frame. The optical signal is converted into an electrical signal through the photodiode receiving board and transmitted to the processor of the main board PCB.

7. A microbial real-time tracking detection and drug sensitivity analysis system according to claim 1, characterized in that: The shell module combines the bottom shell and the outer shell, and fixes the rear cover with a rear locking piece. Four foot pads are used under the bottom shell to support the detection system.

8. A microbial real-time tracking detection and drug sensitivity analysis system, characterized in that: In the XY slide rail module, the same optical fiber is moved by a motor to ensure the consistency of the light source and reduce the detection error.

9. A microbial real-time tracking detection and drug sensitivity analysis system, characterized in that: The main setting parameters are: light source selection, orifice plate selection, circular oscillation rate of 0-1250RPM, detection time interval of 5-360 minutes, detection time of 1-1600 hours, temperature of 15-60℃ (mid-temperature change), dynamic range of 0-5.0 OD, and resolution of 0.001 OD.

10. A microbial real-time tracking detection and drug sensitivity analysis system, characterized in that: The system can be used to evaluate the drug effects of antibiotics on different microbial strains under different environmental conditions. By monitoring the growth of microorganisms and the effects of antibiotics, the optimal dosage and treatment regimen of antibiotics can be determined to improve the treatment effect and reduce the development of antibiotic resistance, and provide important information for the rational use of antibiotics and the management of antibiotic resistance.

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