Disk inoculation method

Abstract

The invention discloses a disc inoculation method which comprises the following steps: connecting an inoculation pipe with one end of a hose through a buckle, and connecting the other end of the hose with a pipe to be inoculated through a buckle; transferring the bacterial liquid from the seed tank to a fermentation tank through a to-be-inoculated pipe, a hose and an inoculation pipe; after transfer is completed, a steam pump is turned on, then a corresponding steam pipe switch on the to-be-inoculated pipe is turned on, steam blowing is conducted on the pipeline, and bacterial liquid remaining in the pipeline is blown away; when bacterial liquid in other seed tanks needs to be transferred, the hoses are connected with the corresponding inoculation pipes and to-be-inoculated pipes through buckles, after connection, the bacterial liquid is transferred to the corresponding fermentation tanks, and after transferring is completed, the pipelines are purged through steam; the key point of the invention lies in that one-to-one inoculation is realized in one system, dead angles of liquid residues and liquid mixing do not occur in the process, and one-to-one accurate butt joint is completed in one system without dead angles.

Description

Technical Field

[0001] The invention belongs to the technical field of lactic acid bacteria transfer and amplification fermentation, and particularly relates to a disc inoculation method. Background Art

[0002] At present, conventional methods on the market all adopt one-to-many inoculation or valve array inoculation. The existing technical methods may cause the risk of bacterial liquid remaining in the system and being easily contaminated. It is a one-to-many transfer method. In addition, after the transfer is completed in the existing inoculation method, the residual bacterial liquid in the pipeline cannot be cleaned out, which leads to cross-infection of bacteria. In addition, the sugar concentration and acidity of the products of traditional bacterial liquid fermentation are relatively low. Therefore, exploring a better bacterial liquid transfer method is a problem with research value. Summary of the invention

[0003] In view of the deficiencies in the prior art, the present invention provides a disc inoculation method.

[0004] A method for disc inoculation comprises the following steps:

[0005] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0006] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0007] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0008] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the tube to be inoculated are connected by snaps. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam.

[0009] Preferably, the steam pressure of the steam purge is 2.0-2.5 MPa.

[0010] Preferably, the culture medium includes one or more of wolfberry, yam, liquorice and kudzu root.

[0011] Preferably, the temperature of the culture medium is 35-37 degrees.

[0012] Preferably, the hose is made of polytetrafluoroethylene.

[0013] Preferably, the inoculation tube and the tube to be inoculated are made of polypropylene.

[0014] Preferably, the culture medium further comprises cellulase.

[0015] Preferably, the culture medium further comprises one or more of adenylic acid, vitamin B, β-glucan, α-glucan, glutamic acid, lysine, serine and vitamin E.

[0016] The technical effects of the present invention are as follows:

[0017] The key point of the present invention is to realize one-to-one inoculation in one system, and there will be no dead corners of liquid residue and cross infection in the process, and the operation is convenient; the residual bacterial liquid in the pipeline can be cleared by steam blowing to avoid infection in the next transfer process. The product of the bacterial liquid fermentation of the present invention has higher acidity and the sugar concentration produced by the fermentation is higher. DETAILED DESCRIPTION

[0018] The technical solutions in the embodiments of the present invention are described clearly and completely. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] A method for disc inoculation comprises the following steps:

[0020] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0021] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0022] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0023] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the tube to be inoculated are connected by snaps. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam.

[0024] Preferably, the steam pressure of the steam purge is 2.0-2.5 MPa.

[0025] Preferably, the culture medium includes one or more of wolfberry, yam, liquorice and kudzu root.

[0026] Preferably, the temperature of the culture medium is 35-37 degrees.

[0027] Preferably, the hose is made of polytetrafluoroethylene.

[0028] Preferably, the inoculation tube and the tube to be inoculated are made of polypropylene.

[0029] Preferably, the culture medium further comprises cellulase.

[0030] The wolfberry, yam, liquorice and kudzu root can promote the fermentation of lactic acid bacteria.

[0031] Comparative Example 1

[0032] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0033] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0034] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0035] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0036] After the bacterial liquid was inoculated into the culture medium, the product of plant straw cellulose enzymatic hydrolysis was used as the carbon source and cultured at 30°C for 40 hours. After the bacterial liquid fermentation was completed, the sugar concentration in the culture medium was tested according to GB 5009.8-2016. The obtained data are shown in Table 1.

[0037] Example 1

[0038] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0039] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0040] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0041] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0042] After the bacterial solution was inoculated into the culture medium, the product of enzymatic hydrolysis of plant straw cellulose was used as the carbon source, wolfberry was added to the culture medium, and cultured at 30°C for 40 hours. After the bacterial solution fermentation was completed, the sugar concentration in the culture medium was tested according to GB 5009.8-2016. The obtained data are shown in Table 1.

[0043] Example 2

[0044] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0045] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0046] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0047] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0048] After the bacterial solution was inoculated into the culture medium, the product of enzymatic hydrolysis of plant straw cellulose was used as the carbon source, Chinese yam was added to the culture medium, and cultured at 30°C for 40 hours. After the bacterial solution fermentation was completed, the sugar concentration in the culture medium was tested according to GB 5009.8-2016, and the obtained data are shown in Table 1.

[0049] Example 3

[0050] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0051] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0052] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0053] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0054] After the bacterial solution was inoculated into the culture medium, the product of enzymatic hydrolysis of plant straw cellulose was used as the carbon source, Chinese yam was added to the culture medium, and cultured at 30°C for 40 hours. After the bacterial solution fermentation was completed, the sugar concentration in the culture medium was tested according to GB 5009.8-2016, and the obtained data are shown in Table 1.

[0055]

[0056] Table 1

[0057] Combining Examples 1-3 and Comparative Example 1 and Table 1, it can be seen that the sugar concentration produced by the bacterial liquid fermentation of the present invention is higher.

[0058] Comparative Example 2

[0059] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0060] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0061] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0062] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0063] After the bacterial liquid was inoculated into the culture medium, the product of enzymatic hydrolysis of plant straw cellulose was used as the carbon source and cultured at 30°C for 40 hours. After the bacterial liquid fermentation was completed, the obtained data were shown in Table 2.

[0064] Example 4

[0065] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0066] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0067] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0068] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0069] After the bacterial solution was inoculated into the culture medium, the product of plant straw cellulose enzymatic hydrolysis was used as the carbon source, adenylic acid was added to the culture medium, and cultured at 30°C for 40 hours. After the bacterial solution fermentation was completed, according to GB

[0070] 5009.239-2016, the measured data are shown in Table 2.

[0071] Example 5

[0072] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0073] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0074] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0075] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0076] After the bacterial solution was inoculated into the culture medium, the product of plant straw cellulose enzymatic hydrolysis was used as the carbon source, vitamin B was added to the culture medium, and cultured at 30°C for 40 hours. After the bacterial solution fermentation was completed, according to GB

[0077] 5009.239-2016, the measured data are shown in Table 2.

[0078] Example 6

[0079] Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle;

[0080] Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium;

[0081] After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe;

[0082] When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the inoculation tube are connected through the buckle. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam;

[0083] After the bacterial solution was inoculated into the culture medium, the product of plant straw cellulose enzymatic hydrolysis was used as the carbon source, β-glucan was added to the culture medium, and cultured at 30°C for 40 hours. After the bacterial solution fermentation was completed, according to GB

[0084] 5009.239-2016, the measured data are shown in Table 2.

[0085]

[0086] Table 2

[0087] It can be seen from Examples 4-6 and Comparative Example 2 and Table 2 that the product of the bacterial liquid fermentation of the present invention has higher acidity.

[0088] The beneficial effects of the present invention are that one-to-one inoculation is achieved within a system, and there will be no dead corners of liquid residue and cross infection during the process, and the operation is convenient; the residual bacterial liquid in the pipeline can be removed by steam blowing to avoid infection during the next transfer process.

[0089] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0090] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A method for disc inoculation, characterized in that: The following steps are involved: Connect the inoculation tube and one end of the hose through a buckle, and connect the other end of the hose to the tube to be inoculated through a buckle; Transferring the bacterial liquid from the seed tank through the inoculation tube, the hose and the inoculation tube to the fermentation tank, which is filled with culture medium; After the transfer is completed, turn on the steam pump, and then turn on the corresponding steam pipe switch on the inoculated tube to steam purge the pipe to clean the bacterial liquid remaining in the pipe; When the bacterial liquid in other seed tanks needs to be transferred, the hose and its corresponding inoculation tube and the tube to be inoculated are connected by snaps. After the connection is completed, the bacterial liquid is transferred to the corresponding fermentation tank. After the transfer is completed, the pipeline is purged with steam.

2. The disc inoculation method according to claim 1, characterized in that: The steam pressure of the steam purge is 2.0-2.5 MPa.

3. The disc inoculation method according to claim 1, characterized in that: The culture medium includes one or more of wolfberry, yam, liquorice and kudzu root.

4. The disc inoculation method according to claim 1, characterized in that: The temperature of the culture medium is 35-37 degrees.

5. The disc inoculation method according to claim 1, characterized in that: The material of the hose is polytetrafluoroethylene.

6. The disc inoculation method according to claim 1, characterized in that: The inoculation tube and the tube to be inoculated are made of polypropylene.

7. The disc inoculation method according to claim 1, characterized in that: The culture medium also includes cellulase.

8. The disc inoculation method according to claim 1, characterized in that: The culture medium further comprises one or more of adenylic acid, vitamin B, β-glucan, α-glucan, glutamic acid, lysine, serine and vitamin E.

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