Automatic detection method, system and medium of fully automatic drug detection instrument

Abstract

The embodiments of the present application relate to an automatic detection method, system and medium of a fully automatic drug detection instrument, and belong to the field of intelligent detection technology. The automatic detection method includes: when the pipette is at the operating point position, sending a liquid level detection instruction to the liquid level detection sensor and obtaining the current liquid level information; when the current liquid level information is inconsistent with the preset empty liquid level information, calculating the vertical movement distance based on the current liquid level information, including: obtaining the first parameter of the corresponding container by looking up the table based on the current operating point position; when the current liquid extraction volume is greater than the first threshold, correcting the current liquid level information; based on the first parameter and the corrected liquid level information, calculating the vertical movement distance, and sending the movement distance to the pipette to control the depth of the pipette extending below the liquid surface; therefore, the automatic detection method can complete an accurate automatic detection process when the detection process requires a variety of different reagents and multiple tests.

Description

Technical Field

[0001] The present application relates to the field of intelligent detection technology, and more specifically, to an automatic detection method, system and medium of a fully automatic drug detection instrument. Background Art

[0002] The activity of drugs such as biological agents is an important evaluation indicator of drugs. However, the activity detection of biological agents such as heparin has complicated steps, and the repeatability and consistency of manual operation are difficult to control, which requires a high level of manual operation proficiency. In order to solve this problem, automatic detection by instruments is used, but it is more difficult to achieve automatic detection. The existing automatic detection equipment is operated by a robotic arm under the cooperation of instructions, but the operation of the robotic arm can only complete a series of operations within the length of the robotic arm, and cannot complete more complex operations. For example, reagents may include test samples, standard samples, chromogenic substrates, buffer solutions, etc. The operations that need to be completed may exceed the operating range of the robotic arm and have poor accuracy. Summary of the invention

[0003] The present application is provided to solve the above defects existing in the prior art. An automatic detection method, system and medium of a fully automatic drug detection instrument are needed, which can accurately complete the automatic detection process under various reagent conditions and improve the precision of operation and the accuracy of detection.

[0004] In a first aspect of the present application, there is provided an automatic detection method for a fully automatic drug detection instrument, the automatic detection method comprising: configuring target position information for a pipette so that the pipette moves automatically according to the target position information; obtaining real-time position information of the pipette; when the pipette is at an operating point position, issuing a liquid level detection instruction to a liquid level detection sensor and obtaining current liquid level information; based on the current liquid level information, in the case where the current liquid level information is inconsistent with preset empty liquid level information, calculating a vertical movement distance based on the current liquid level information, comprising: obtaining a first parameter of a corresponding container by looking up a table based on the current sampling point position; when taking liquid, based on the current amount of liquid taken, in the case where the current amount of liquid taken is greater than a first threshold, correcting the current liquid level information; calculating a vertical movement distance based on the first parameter and the corrected liquid level information, and sending the movement distance to the pipette to control the depth of the pipette extending below the liquid surface.

[0005] According to a second aspect of the present application, there is provided an automatic detection system for a fully automatic drug detection instrument, the automatic detection system comprising a pipette, a control module, a liquid level detection sensor, the control module being configured to: configure target position information for the pipette so that the pipette automatically moves according to the target position information; obtain real-time position information of the pipette; when the pipette is at an operating point position, send a liquid level detection instruction to the liquid level detection sensor and obtain current liquid level information; based on the current liquid level information, when the current liquid level information is inconsistent with preset empty liquid level information, calculate a vertical movement distance based on the current liquid level information, including: obtaining a first parameter of a corresponding container by looking up a table based on the current sampling point position; when taking liquid, based on the current amount of liquid taken, when the current amount of liquid taken is greater than a first threshold, correct the current liquid level information; based on the first parameter and the corrected liquid level information, calculate the vertical movement distance, and send the movement distance to the pipette to control the depth of the pipette extending below the liquid surface.

[0006] The third aspect of the present application provides a non-transitory computer-readable medium having instructions stored thereon, which, when executed by a processor, executes the steps of the automatic detection method of the fully automatic drug detection instrument as described in any embodiment of the present application.

[0007] The automatic detection method, system and medium of the fully automatic drug detection instrument provided in each embodiment of the present application configure position information for the pipette so that the pipette can obtain the target position and can automatically move to different positions, thereby completing the processes of automatically taking the pipette tip, taking the liquid, and releasing the liquid. When taking the liquid, the liquid level is first detected to avoid taking the liquid when the liquid level is empty, thereby improving accuracy; the liquid level height is determined based on the liquid amount taken last time, thereby avoiding the pipette tip from extending too far below the liquid level or not reaching the liquid level. The automatic detection process can be accurately completed when a variety of different reagents and multiple tests are required in the detection process, and the accuracy of the operation and the accuracy of the detection can also be improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] In the drawings, which are not necessarily drawn to scale, the same reference numerals may describe similar parts in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar parts. The drawings generally illustrate various embodiments by way of example and not limitation, and together with the specification, are used to illustrate the embodiments applied for. When appropriate, the same reference numerals are used throughout the drawings to refer to the same or similar parts. Such embodiments are illustrative and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.

[0009] Figure 1A flow chart showing an automatic detection method of a fully automatic drug detection instrument according to an embodiment of the present application;

[0010] Figure 2 A schematic diagram showing the structure of an automatic detection system of a fully automatic drug detection instrument according to an embodiment of the present application;

[0011] Figure 3 A schematic structural diagram of a fully automatic drug detection instrument according to an embodiment of the present application is shown. DETAILED DESCRIPTION

[0012] In order to enable those skilled in the art to better understand the technical solution of the present application, the present application is described in detail below in conjunction with the accompanying drawings and specific implementation examples. The embodiments of the present application are further described in detail below in conjunction with the accompanying drawings and specific implementation examples, but are not intended to limit the present application.

[0013] The words "first", "second" and similar words used in this application do not indicate any order, quantity or importance, but are only used to distinguish. The words "include" or "comprise" and similar words mean that the elements before the word include the elements listed after the word, and do not exclude the possibility of including other elements.

[0014] According to an embodiment of the present application, an automatic detection method of a fully automatic drug detection instrument is provided, and the automatic detection method includes the following steps.

[0015] In step 101, the target position information is configured for the pipette so that the pipette moves automatically according to the target position information. In this way, the pipette can obtain the specific target position to be moved to, and then it can move automatically to ensure that it reaches the accurate position. The pipette can be a pipette gun, and the target position can refer to the position of the gun tip, the position of each different reagent, the position of the oscillator, etc.

[0016] In step 102, the real-time position information of the pipette is obtained. In step 103, when the pipette is at the operating point, a liquid level detection instruction is sent to the liquid level detection sensor, and the current liquid level information is obtained. When the pipette reaches the liquid taking position, it means that the pipette is about to take liquid, so the liquid level detection is first performed to obtain the current liquid level information.

[0017] In step 104, based on the current liquid level information, if the current liquid level information is inconsistent with the preset empty liquid level information, the vertical moving distance is calculated based on the current liquid level information. This can avoid empty liquid collection when there is no liquid level, resulting in the false impression that liquid has been collected, and can improve the accuracy of detection.

[0018] In step 1041, the first parameter of the corresponding container is obtained by looking up the table based on the current operation point position. The containers for holding solutions at different operation points are different, so the heights are different, which facilitates determining the height of the pipette and the liquid level and subsequently determining the end point position of the movement.

[0019] In step 1042, when taking liquid, based on the current amount of liquid taken, when the current amount of liquid taken is greater than the first threshold value, the current liquid level information is corrected. Because the amount of liquid taken each time is about 5mL, if the amount of liquid taken is large, the liquid level changes greatly, and the liquid level detection sensor has a certain error. The pipette takes liquid through the pipette tip, and the pipette is connected to the pipette tip. The height of the pipette tip is about 5cm. When taking liquid or releasing liquid, the pipette tip needs to extend below the liquid level, so that the pipette tip will be stained with part of the liquid, which may affect the accuracy of the detection. Therefore, the bottom of the pipette tip is controlled to extend 3-4mm below the liquid level. In the process of taking liquid, the liquid level will also have a downward fluctuation. Therefore, in order to accurately control the pipette tip to extend below the liquid level, it is necessary to set parameters according to the change of the amount of liquid taken and the current amount of liquid taken, and correct the current liquid level information.

[0020] In step 1043, based on the first parameter and the corrected liquid level information, the vertical moving distance is calculated and sent to the pipette to control the depth of the pipette under the liquid surface. In this way, the depth of the pipette can be accurately controlled. Knowing the height of the pipette tip from the top of the container, and then combining the height of the liquid surface, the distance the pipette moves downward can be calculated.

[0021] Therefore, the processes of taking liquid, preparing liquid and testing are performed in sequence according to the position reached by the pipette to accurately complete the testing process, and the automatic testing process can be accurately completed when the testing process requires a variety of different reagents and multiple tests.

[0022] In some embodiments, based on the current liquid withdrawal amount, when the current liquid withdrawal amount is greater than a first threshold, the current liquid level information is corrected, including: using formula (1) to obtain the corrected liquid level information,

[0023] H = h - ((L × σ) / 5) (1)

[0024] Wherein, L represents the current liquid volume, h represents the currently detected liquid level, and σ represents the coefficient, and its value range is 0.8-1.

[0025] The first threshold value can be 3mL, etc. The value of σ can be selected according to the type of solution. For solutions with higher viscosity, a larger value is selected. It can also be determined according to the current amount of liquid taken. The larger the current amount of liquid taken, the larger σ is selected. For example, if 10cm is detected, 3mL was taken last time, and σ is 0.8, then H=9.62cm. In this way, the height of the pipette tip descending can be controlled, the accuracy of the liquid level height can be improved, and it can also ensure that the depth of the pipette tip inserted into the liquid surface will not fluctuate greatly, so that the liquid can be taken, and the amount taken will not be less than the predetermined amount due to the fluctuation of the liquid level.

[0026] In some embodiments, target position information is configured for the pipette so that the pipette moves automatically according to the target position information, including: obtaining the position information of each target point; using the pipette as the center point of the three-dimensional coordinate system, and based on the position information of each target point, obtaining the target coordinate point information of each target point in the three-dimensional coordinate system; sending the target coordinate point information of the liquid preparation point and each liquid collection point to the pipette, so that the pipette moves according to the target coordinate point information.

[0027] The position information of each target point can be manually input information and stored in advance. Then, the initial position of the pipette is used as the center point of the three-dimensional coordinate system to obtain the relative distance of each target point relative to the initial position of the pipette, so that the target coordinate point information of each target point in the three-dimensional coordinate system, the information in the X direction, the information in the Y direction, and the information in the Z direction can be obtained, and then the pipette can be moved conveniently. In some embodiments, the automatic detection method also includes: obtaining the X-axis coordinate information, the Y-axis coordinate information and the Z-axis coordinate information of the target point according to the target coordinate point information; and moving in sequence according to the X-axis coordinate information, the Y-axis coordinate information and the Z-axis coordinate information. For example, if the target coordinates are (1000, 300, 200), the X-axis is first moved to 1000, then the Y-axis is moved to 300, and then the Z-axis is moved to 200. Since there are many types of reagents and the dispensing points are in different positions, the precise positioning of the target position information facilitates the pipette to accurately complete the detection process. The pipette can select the middle position between the top of the pipette and the top of the container containing the solution as the end position after the move.

[0028] In some embodiments, the automatic detection method further comprises: obtaining the real-time position information of the pipette and converting it into the real-time coordinate information in the three-dimensional coordinate system, and issuing an alarm message when the real-time coordinate information is not within the range of the target coordinate point information. The position information of the pipette can be obtained by a position sensor, for example, a position sensor is provided on the pipette. The real-time position information of the pipette can be obtained at intervals of a first time interval, for example, 5s. If the real-time coordinate information is within the range of the target coordinate point information, it means that the pipette is in the normal pipetting process, and no alarm is required, and the movement can be continued. If the real-time coordinate information is not within the range of the target coordinate point information, it means that the normal path of the pipette deviates, and an alarm message is issued.

[0029] In some embodiments, when the real-time coordinate information is within the range of the target coordinate point information, issuing an alarm message includes:

[0030] The coordinate values ​​of the X, Y and Z axes in the real-time coordinate information are compared with the coordinate values ​​of the X, Y and Z axes in the target coordinate point information. When the coordinate values ​​of the X, Y and Z axes in the real-time coordinate information are greater than the coordinate values ​​of the X, Y and Z axes in the target coordinate point information, an alarm message is issued. For example, if the target coordinates are (1000, 300, 200) and the real-time position information is (1000, 200, 0), it means that the real-time coordinate information is within the range of the target coordinate point information.

[0031] In some embodiments, the pipette returns to the initial position after moving to a target point, and then moves to the next target point, so as to avoid position movement errors caused by problems such as recognition of coordinate information when moving directly from one target point to the next target point.

[0032] In some embodiments, when the current liquid level information and the preset empty liquid level information are inconsistent, a liquid taking instruction is issued to the pipette, including: the current liquid level information and the empty liquid level information are the current voltage value and the empty liquid level voltage value respectively, and when the current voltage value and the empty liquid level voltage value are inconsistent, a liquid taking instruction is issued to the pipette. A voltage converter is provided inside the liquid level detection sensor, and after the liquid level detection sensor detects the liquid level, the voltage converter will output a corresponding voltage value, so as to automatically determine the liquid level condition.

[0033] In some embodiments, the automatic detection method further comprises: when the current liquid level information is consistent with the preset empty liquid level information, issuing a reminder message for adding liquid. If the liquid level is empty, no liquid is taken and a reminder is issued so that the staff can replenish the liquid in time.

[0034] The operation points include liquid collection points, liquid preparation points and / or detection points, etc.

[0035] When the pipette is at the liquid dispensing point, a liquid dispensing instruction is sent to the liquid dispensing device. After the liquid dispensing operation is completed, a liquid taking instruction is sent to the pipette. The liquid dispensing device may include an oscillator or a heater, etc. When the pipette is at the liquid dispensing point, the oscillator oscillates according to the liquid dispensing instruction to fully mix the liquid, or the heater heats the liquid according to the liquid dispensing instruction. When the pipette is at the detection point, a detection instruction is sent to the detection equipment so that the detection equipment performs detection and obtains the detection result. The detection equipment may be an optical analysis system, which can obtain an optical analysis result.

[0036] According to an embodiment of the present application, an automatic detection system of a fully automatic drug detection instrument is also provided, the automatic detection system 200 includes a pipette 201, a control module 202, and a liquid level detection sensor 203, the control module 202 is configured to: configure target position information for the pipette so that the pipette moves automatically according to the target position information; obtain real-time position information of the pipette; when the pipette is at the operating point position, send a liquid level detection instruction to the liquid level detection sensor and obtain current liquid level information; based on the current liquid level information, when the current liquid level information is inconsistent with the preset empty liquid level information, calculate the vertical movement distance based on the current liquid level information, including: obtaining a first parameter of the corresponding container by looking up a table based on the current sampling point position; when taking liquid, based on the current liquid taking amount, when the current liquid taking amount is greater than a first threshold, correct the current liquid level information; based on the first parameter and the corrected liquid level information, calculate the vertical movement distance, and send the movement distance to the pipette to control the depth of the pipette extending below the liquid surface.

[0037] like Figure 3 As shown, different reagents, gun tips, and containers 205 (containing reagents, etc.) are placed in different positions, and the pipette is located at the top. Through the execution steps of the control module, the automatic detection process can be better completed accurately based on the fully automatic drug detection instrument. The control module 202 is connected to the pipette 201 and the liquid level detection sensor 203 to transmit signals.

[0038] In some embodiments, the automatic detection system further includes a display module 204. To display the detection results, the detection progress, etc., the display module 204 can also be configured with a touch screen to facilitate the operator to perform corresponding operations. The relevant information of the control module 202 can be output to the control module 202 for display.

[0039] In some embodiments, the automatic detection system 200 further includes a position sensor, such as a position sensor provided on a pipette, and the position information of the pipette is obtained through the position sensor.

[0040] In some embodiments, target position information is configured for a pipette so that the pipette moves automatically according to the target position information, including: obtaining position information of each target point; using the pipette as the center point of a three-dimensional coordinate system, and based on the position information of each target point, obtaining target coordinate point information of each target point in the three-dimensional coordinate system; and sending the target coordinate point information of each target point to the pipette so that the pipette moves according to the target coordinate point information.

[0041] In some embodiments, the control module 202 is further configured to obtain the real-time position information of the pipette and convert it into real-time coordinate information in a three-dimensional coordinate system, and issue an alarm message when the real-time coordinate information is not within the range of the target coordinate point information.

[0042] According to an embodiment of the present application, a non-transitory computer-readable medium is also provided, on which instructions are stored. When executed by a processor, the instructions execute the steps of the automatic detection method of the fully automatic drug detection instrument as described in any embodiment of the present application.

[0043] In addition, although exemplary embodiments have been described herein, their scope includes any and all embodiments based on the present application with equivalent elements, modifications, omissions, combinations (e.g., various embodiments intersecting schemes), adaptations or changes. Therefore, this specification and examples are intended to be considered as examples only.

[0044] The above description is intended to be illustrative rather than restrictive. For example, the above examples (or one or more of them) may be used in combination with each other. For example, a person of ordinary skill in the art may use other embodiments when reading the above description. In addition, in the above specific embodiments, various features may be grouped together to simplify the present application. On the contrary, the subject matter of the present application may be less than all the features of the embodiments of a particular application.

[0045] The above embodiments are only exemplary embodiments of the present invention and are not intended to limit the present invention. Those skilled in the art may make various modifications or equivalent substitutions to the present invention within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present invention.

Claims

1. An automatic detection method for a fully automatic drug detection instrument, characterized in that: The automatic detection method comprises: Configuring target position information for the pipette so that the pipette automatically moves according to the target position information; Get real-time pipette position information; When the pipette is at the operating point, a liquid level detection instruction is sent to the liquid level detection sensor, and current liquid level information is obtained; Based on the current liquid level information, when the current liquid level information is inconsistent with the preset empty liquid level information, the vertical moving distance is calculated based on the current liquid level information, including: obtaining the height of the corresponding container by looking up a table based on the current operation point position; when taking liquid, based on the current liquid taking amount, when the current liquid taking amount is greater than a first threshold, the current liquid level information is corrected, including: using formula (1) to obtain the corrected liquid level information, H=h-((L×σ) / 5) (1); wherein L represents the current liquid taking amount, h represents the current liquid level height, and σ represents a coefficient, with a value range of 0.8-1; based on the height of the container and the corrected liquid level information, the vertical moving distance is calculated, and the moving distance is sent to the pipette to control the depth of the pipette extending below the liquid surface.

2. The automatic detection method according to claim 1, characterized in that: Configuring target position information for a pipette so that the pipette automatically moves according to the target position information, including: Get the location information of each target point; Taking the pipette as the center point of the three-dimensional coordinate system, based on the position information of each target point, the target coordinate point information of each target point in the three-dimensional coordinate system is obtained; The target coordinate point information of each target point is sent to the pipette, so that the pipette moves according to the target coordinate point information.

3. The automatic detection method according to claim 2, characterized in that: The automatic detection method further comprises: acquiring real-time position information of the pipette and converting it into real-time coordinate information in a three-dimensional coordinate system, and issuing an alarm message when the real-time coordinate information is not within the range of the target coordinate point information.

4. The automatic detection method according to claim 3, characterized in that: When the real-time coordinate information is within the range of the target coordinate point information, an alarm message is issued, including: The coordinate values ​​of the X, Y and Z axes in the real-time coordinate information are compared with the coordinate values ​​of the X, Y and Z axes in the target coordinate point information. When the coordinate values ​​of the X, Y and Z axes in the real-time coordinate information are greater than the coordinate values ​​of the X, Y and Z axes in the target coordinate point information, an alarm message is issued.

5. The automatic detection method according to claim 1, characterized in that: When the current liquid level information is consistent with the preset empty liquid level information, a reminder message for adding liquid is issued.

6. An automatic detection system for a fully automatic drug detection instrument, characterized in that: The automatic detection system includes a pipette, a control module and a liquid level detection sensor, and the control module is configured as follows: Configuring target position information for the pipette so that the pipette automatically moves according to the target position information; Get real-time pipette position information; When the pipette is at the operating point, a liquid level detection instruction is sent to the liquid level detection sensor, and current liquid level information is obtained; Based on the current liquid level information, when the current liquid level information is inconsistent with the preset empty liquid level information, the vertical moving distance is calculated based on the current liquid level information, including: obtaining the height of the corresponding container by looking up a table based on the current operation point position; when taking liquid, based on the current liquid taking amount, when the current liquid taking amount is greater than a first threshold, the current liquid level information is corrected, including: using formula (1) to obtain the corrected liquid level information, H=h-((L×σ) / 5) (1); wherein L represents the current liquid taking amount, h represents the current liquid level height, and σ represents a coefficient, with a value range of 0.8-1; based on the height of the container and the corrected liquid level information, the vertical moving distance is calculated, and the moving distance is sent to the pipette to control the depth of the pipette extending below the liquid surface.

7. The automatic detection system according to claim 6, characterized in that: Configuring target position information for a pipette so that the pipette automatically moves according to the target position information, including: Get the location information of each target point; Taking the pipette as the center point of the three-dimensional coordinate system, based on the position information of each target point, the target coordinate point information of each target point in the three-dimensional coordinate system is obtained; The target coordinate point information of each target point is sent to the pipette, so that the pipette moves according to the target coordinate point information.

8. The automatic detection system according to claim 6, characterized in that: The control module is also configured to obtain real-time position information of the pipette and convert it into real-time coordinate information in a three-dimensional coordinate system, and issue an alarm message when the real-time coordinate information is not within the range of the target coordinate point information.

9. A non-transitory computer-readable medium having instructions stored thereon, which, when executed by a processor, execute the steps of the automatic detection method of the fully automatic drug detection instrument as described in any one of claims 1 to 5.

Images