Plant source-derived volatile mosquito repellent liquid for cats and preparation method of plant source-derived volatile mosquito repellent liquid

Abstract

The invention discloses a plant-derived volatile mosquito repellent liquid for cats and a preparation method thereof. The mosquito repellent liquid comprises the following components in percentage by mass: 15-40% of plant essential oil, 0.02-0.2% of an antioxidant and 60-85% of a solvent. According to the cat mosquito repellent, the plant essential oil which is safe and harmless to cats and people is adopted to replace pyrethrin which is common in the market and serves as an effective mosquito repellent ingredient, the mosquito repellent effect is guaranteed, meanwhile, adverse effects on cat health are avoided, the plant essential oil is low in price and easy to obtain, the preparation method is simple, and the cat mosquito repellent is suitable for industrial production.

Description

Technical Field

[0001] The invention belongs to the field of pet health care, and in particular relates to a plant-derived volatile mosquito repellent liquid for cats and a preparation method thereof. Background Art

[0002] Summer is the period when mosquitoes are most active. People's normal lives are affected by mosquitoes, and mosquito bites can also spread various diseases, posing a serious threat to people's health. Currently, widely used mosquito repellent products include mosquito coils, electric mosquito coils, electric mosquito repellent liquids, etc. However, the active ingredients of these mosquito repellent products are all pyrethroids, which are slightly toxic to humans. At the same time, for pet cats in cat-raising families, since cats lack glucuronyl transferase in their bodies and cannot metabolize pyrethroids by themselves, long-term use will have varying degrees of impact on the health of cats, ranging from vomiting to poisoning. Therefore, it is necessary to develop a mosquito repellent that will not affect the health of cats and humans. Summary of the invention

[0003] The technical problem to be solved by the present invention is to address the deficiencies of the above-mentioned prior art and to provide a plant-derived volatile mosquito repellent liquid for cats and a preparation method thereof. Plant essential oils that are safe and harmless to cats and humans are used to replace the common pyrethroids on the market as the effective ingredient for mosquito repellents. While ensuring the mosquito repellent effect, it will not have an adverse effect on the health of cats. In addition, plant essential oils are cheap and easily available, and the preparation method is simple, which is suitable for industrial production.

[0004] The technical scheme adopted by the present invention is: a plant-derived volatile mosquito repellent liquid for cats, which comprises the following components by mass percentage: 15% to 40% of plant essential oil, 0.02% to 0.2% of antioxidant and 60% to 85% of solvent.

[0005] In one of the embodiments, the mosquito repellent liquid comprises the following components by mass percentage: 30% plant essential oil, 0.1% antioxidant and 69.9% solvent.

[0006] In one embodiment, the plant essential oil is at least one of catnip essential oil, wormwood essential oil or perilla leaf essential oil.

[0007] In one embodiment, the plant essential oil comprises the following components by weight: 15-40 parts of catnip, 10-30 parts of wormwood leaves and 13-30 parts of perilla leaves.

[0008] In one embodiment, the plant essential oil comprises the following components by weight: 33 parts of catnip, 30 parts of wormwood leaves and 27 parts of perilla leaves.

[0009] In one embodiment, the antioxidant is at least one of BHT, BHA, TBHQ or tocopherol.

[0010] The present invention also discloses a method for preparing a plant-derived volatile mosquito repellent liquid for cats, which is specifically as follows:

[0011] Step 1, weighing the raw materials required for the plant essential oil according to parts by weight for later use, and weighing the antioxidant and solvent according to mass percentage for later use;

[0012] Step 2, respectively extracting catnip essential oil, wormwood essential oil and perilla leaf essential oil;

[0013] Step 3, mixing the plant essential oil extracted in step 2 with the antioxidant, stirring evenly to completely dissolve the antioxidant, and obtaining a semi-finished product solution;

[0014] Step 4: Add a solvent to the semi-finished solution obtained in step 3, stir evenly, and obtain a plant-derived volatile mosquito repellent liquid for cats.

[0015] In one embodiment, the extraction of catnip essential oil is as follows:

[0016] The catnip is washed with distilled water and then chopped, placed in a volatile oil extraction container, and steam distilled for 4 hours. The upper oil layer in the extraction container is the catnip volatile oil. The catnip essential oil is obtained by collecting the upper oil layer.

[0017] In one embodiment, the extraction of wormwood essential oil is as follows:

[0018] Take the wormwood leaves, grind them and place them in a 2000 ml round-bottom flask, add 5 to 8 times the amount of water and soak them for 8 hours. After boiling, adjust the heat to medium and keep it slightly boiling. Stop heating after 6 to 10 hours, let it stand for 20 minutes, collect the oil layer in the round-bottom flask, and obtain wormwood essential oil.

[0019] In one embodiment, the extraction of perilla leaf essential oil is as follows:

[0020] Dry the perilla leaves naturally, crush them, pass through an 80-mesh sieve, place them in a 1000ml round-bottom distillation flask, add 600ml of distilled water, add a few glass beads, mix thoroughly, and soak them in a 50℃ water bath for 2h;

[0021] After soaking in a water bath, transfer to an extraction bottle, place in a 45°C constant temperature water bath, and use petroleum ether as an extractant for 4 hours for distillation extraction to obtain a petroleum ether extract;

[0022] The petroleum ether extract was taken out, and the oil and water were separated using a separatory funnel. About 15 g of anhydrous sodium sulfate was added, and the extract was allowed to stand for dehydration, and then concentrated under reduced pressure at 40° C. After the solvent was evaporated, the perilla leaf essential oil was obtained.

[0023] The beneficial effects of the present invention are as follows: the plant for cats of the present invention is derived from a volatile mosquito repellent liquid, and the plant essential oil that is safe and harmless to cats and humans is used to replace the common pyrethroids as the effective ingredient for mosquito repellent on the market, while ensuring the mosquito repellent effect without having an adverse effect on the health of cats, and the plant essential oil is cheap and easy to obtain, the preparation method is simple, and it is suitable for industrial production. DETAILED DESCRIPTION

[0024] The present invention will be further described in detail below with reference to specific embodiments.

[0025] Embodiment 1:

[0026] The invention discloses a plant-derived volatile mosquito repellent liquid for cats. The mosquito repellent liquid comprises the following components by mass percentage: 30% of plant essential oil, 0.1% of antioxidant and 69.9% of solvent.

[0027] The invention discloses a method for preparing a plant-derived volatile mosquito repellent liquid for cats, comprising the following steps:

[0028] Weigh the raw materials required for the plant essential oil according to the weight percentage and set aside, and weigh the antioxidant and the solvent according to the mass percentage and set aside;

[0029] Extracting catnip essential oil: Wash catnip with distilled water and chop it into small pieces, put it in a volatile oil extraction container, and use steam distillation for 4 hours. The upper oil layer in the extraction container is catnip volatile oil. Collect the upper oil layer to obtain catnip essential oil;

[0030] Extracting wormwood essential oil: crush wormwood and place it in a 2000 ml round-bottom flask, add 5 to 8 times water and soak for 8 hours, boil and then adjust the heat to keep it slightly boiling, stop heating after 6 to 10 hours, let it stand for 20 minutes, collect the oil layer in the round-bottom flask, and obtain wormwood essential oil;

[0031] Extracting perilla leaf essential oil: drying the perilla leaf naturally, crushing it, passing it through an 80-mesh sieve, placing it in a 1000 ml round-bottom distillation bottle, adding 600 ml of distilled water, adding a few glass beads, and soaking it in a 50° C. water bath for 2 hours after fully mixing; after soaking in the water bath, moving it to an extraction bottle, placing it in a 45° C. constant temperature water bath, and distilling and extracting it with petroleum ether as an extractant for 4 hours to obtain a petroleum ether extract; taking out the petroleum ether extract, using a separatory funnel to separate oil and water, adding about 15 g of anhydrous sodium sulfate, standing it for dehydration, and concentrating it under reduced pressure at 40° C. After the solvent is evaporated, the perilla leaf essential oil is obtained;

[0032] The extracted plant essential oil is mixed with the antioxidant, and stirred evenly to completely dissolve the antioxidant to obtain a semi-finished product solution;

[0033] A solvent is added to the obtained semi-finished product solution, and the mixture is stirred evenly to obtain a plant-derived volatile mosquito repellent liquid for cats.

[0034] Embodiment 2:

[0035] The invention discloses a plant-derived volatile mosquito repellent liquid for cats. The mosquito repellent liquid comprises the following components by mass percentage: 25% of plant essential oil, 0.1% of antioxidant and 74.9% of solvent.

[0036] The preparation method is the same as that of Example 1.

[0037] Embodiment 3:

[0038] The invention discloses a plant-derived volatile mosquito repellent liquid for cats. The mosquito repellent liquid comprises the following components by mass percentage: 35% of plant essential oil, 0.1% of antioxidant and 64.9% of solvent.

[0039] The preparation method is the same as that of Example 1.

[0040] In the above technical scheme, catnip, also known as camphor grass, small nepeta, etc., has volatile oils mainly composed of terpenes, from which a mosquito repellent active ingredient, nepetalactone, can be separated and extracted. Studies have shown that the mosquito repellent activity of nepetalactone is 10 times that of DEET. At the same time, toxicity evaluation tests have shown that catnip volatile oil is non-toxic, non-irritating to the skin, and non-allergenic. Perilla contains perilla aldehyde, limonene, linalool, menthol, perilla oil, etc., which have the effects of repelling mosquitoes and insects, clearing away heat and detoxifying. Artemisia argyi is the leaf of the wormwood plant of the Asteraceae family, and its special smell has the effect of repelling mosquitoes and insects.

[0041] The present invention adopts plant essential oil which is safe and harmless to cats as the effective ingredient for repelling mosquitoes, and will not affect the health of cats and humans. Compared with mosquito repellent products circulating in the market which use pyrethroids as mosquito repellent ingredients, the present invention is safer. In addition, by utilizing the volatility of essential oil, compared with mosquito repellent products which need to be powered on and ignited, the present invention is more energy-saving and environmentally friendly while ensuring the mosquito repellent effect.

[0042] The method for testing the above cat-use plant-derived volatile mosquito repellent is as follows:

[0043] Test 1: Indoor efficacy

[0044] The test was conducted using the repellent method in Part 9 of GB / T 13917.9-2009. Indoor conditions: 26±1°C, relative humidity 65±10%. The comparative example was a commercially available mosquito repellent. The results are shown in Table 1.

[0045] Table 1 Repelling schedule of different embodiments

[0046] Group Aedes albopictus Embodiment 1 6.5h Embodiment 2 6h Embodiment 3 7.5h Comparative Example 9.5h

[0047] Conclusion: From Table 1, it can be seen that the effective protection time of the plant-derived volatile mosquito repellent for cats of the present invention increases with the increase of the essential oil concentration. When the concentration reaches 25%, it reaches the minimum protection time of Class A in the national standard of 6 hours.

[0048] Test 2: Security

[0049] (1) Acute inhalation toxicity test:

[0050] The mosquito repellent liquids of the above three embodiments were placed in a static poisoning cabinet and electrically volatilized, and then three mice were put in respectively. The acute inhalation toxicity experiment on mice in accordance with GB15670-1995 Toxicology Test Method for Pesticide Registration was carried out. The test results are as follows: after poisoning, the mice all showed symptoms such as sweating, slow breathing, reduced activity, and sluggish reaction, and no mice died. The LD50 was greater than 10g / m3. According to the Acute Inhalation Toxicity Grading Standard for Pesticides, the toxicity and grading standards of 1.2 and 1.2.8 of the Ministry of Agriculture were all judged to be non-toxic.

[0051] The above-mentioned embodiments only express the specific implementation of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A plant-derived volatile mosquito repellent for cats, characterized in that: The mosquito repellent liquid comprises the following components by mass percentage: 15% to 40% of plant essential oil, 0.02% to 0.2% of antioxidant and 60% to 85% of solvent.

2. The plant-derived volatile mosquito repellent for cats according to claim 1, characterized in that: The mosquito repellent liquid comprises the following components by mass percentage: 30% of plant essential oil, 0.1% of antioxidant and 69.9% of solvent.

3. A plant-derived volatile mosquito repellent for cats according to claim 1 or 2, characterized in that: The plant essential oil is at least one of catnip essential oil, wormwood essential oil or perilla leaf essential oil.

4. The plant-derived volatile mosquito repellent for cats according to claim 3, characterized in that: The plant essential oil comprises the following components by weight: 15-40 parts of catnip, 10-30 parts of wormwood leaves and 13-30 parts of perilla leaves.

5. The plant-derived volatile mosquito repellent for cats according to claim 4, characterized in that: The plant essential oil comprises the following components by weight: 33 parts of catnip, 30 parts of wormwood leaves and 27 parts of perilla leaves.

6. A plant-derived volatile mosquito repellent for cats according to claim 1, 2, 4 or 5, characterized in that: The antioxidant is at least one of BHT, BHA, TBHQ or tocopherol.

7. A method for preparing a plant-derived volatile mosquito repellent for cats, characterized in that: The details are as follows: Step 1, weighing the raw materials required for the plant essential oil according to parts by weight for later use, and weighing the antioxidant and solvent according to mass percentage for later use; Step 2, respectively extracting catnip essential oil, wormwood essential oil and perilla leaf essential oil; Step 3, mixing the plant essential oil extracted in step 2 with the antioxidant, stirring evenly to completely dissolve the antioxidant, and obtaining a semi-finished product solution; Step 4: Add a solvent to the semi-finished solution obtained in step 3, stir evenly, and obtain a plant-derived volatile mosquito repellent liquid for cats.

8. The method for preparing a plant-derived volatile mosquito repellent for cats according to claim 7, characterized in that: The extraction of catnip essential oil is specifically as follows: The catnip is washed with distilled water and then chopped, placed in a volatile oil extraction container, and steam distilled for 4 hours. The upper oil layer in the extraction container is the catnip volatile oil. The catnip essential oil is obtained by collecting the upper oil layer.

9. The method for preparing a plant-derived volatile mosquito repellent for cats according to claim 7, characterized in that: The extraction of wormwood essential oil is as follows: Take the wormwood leaves, grind them and place them in a 2000 ml round-bottom flask, add 5 to 8 times the amount of water and soak them for 8 hours. After boiling, adjust the heat to medium and keep it slightly boiling. Stop heating after 6 to 10 hours, let it stand for 20 minutes, collect the oil layer in the round-bottom flask, and obtain wormwood essential oil.

10. The method for preparing a plant-derived volatile mosquito repellent for cats according to claim 7, characterized in that: The extraction of perilla leaf essential oil is specifically as follows: Dry the perilla leaves naturally, crush them, pass through an 80-mesh sieve, place them in a 1000ml round-bottom distillation flask, add 600ml of distilled water, add a few glass beads, mix thoroughly, and soak them in a 50℃ water bath for 2h; After soaking in a water bath, transfer to an extraction bottle, place in a 45°C constant temperature water bath, and use petroleum ether as an extractant for 4 hours for distillation extraction to obtain a petroleum ether extract; The petroleum ether extract was taken out, and the oil and water were separated using a separatory funnel. About 15 g of anhydrous sodium sulfate was added, and the extract was allowed to stand for dehydration, and then concentrated under reduced pressure at 40° C. After the solvent was evaporated, the perilla leaf essential oil was obtained.