Functional composition and complete food for kidneys of dogs and cats and preparation method of functional composition and complete food

Abstract

The invention discloses a functional composition and complete food for kidneys of dogs and cats and a preparation method of the functional composition and the complete food, and relates to the technical field of animal feed. The functional composition comprises a plant extract, prebiotics and probiotics. The plant extract consists of an astragalus extract, a rhodiola rosea extract, a centella asiatica extract, a gynostemma pentaphylla extract and a poria cocos extract. The prebiotics are composed of fucoidan, synanthrin and lactulose. The probiotics are prepared from streptococcus thermophilus, lactobacillus acidophilus and bifidobacterium longum. According to the functional composition and the complete food for the kidney of the dog and the cat and the preparation method of the functional composition and the complete food, plant extracts, prebiotics and probiotics are selected to be matched, and the functional composition and the complete food have the anti-oxidation, anti-inflammatory and anti-fibrosis effects on the kidney of the dog and the cat. The complete food containing the functional composition for the kidneys of the dogs and the cats is applied to pet food, so that the kidney injury of the dogs and the cats can be prevented or the disease progress of chronic kidney diseases of the dogs and the cats can be delayed.

Description

Technical Field

[0001] The invention relates to the technical field of animal feed, in particular to a functional composition and complete food for dog and cat kidneys and a preparation method thereof. Background Art

[0002] Kidney diseases are very common in elderly dogs and cats, among which chronic kidney disease (CKD) is a syndrome of irreversible loss of renal tissue function caused by various kidney diseases. In the pathological process of CKD, kidney damage causes the loss of nephrons. In order to repair the damage, infiltrating immune cells, proteinuria and diabetes promote tubular interstitial inflammation and fibrosis. The increase in the workload of tubular transport of residual nephrons also involves anaerobic metabolism, intracellular acidosis, endoplasmic reticulum stress and oxidative stress. Therefore, the hallmark pathological changes of CKD at the molecular level are oxidative stress, inflammation and fibrosis of renal tissue.

[0003] In addition, in recent years, the "gut-kidney axis" theory has gradually gained attention. Due to the loss of renal function, the nitrogenous waste produced by metabolism in the body cannot be excreted from the body, and accumulates in the body and becomes toxic, forming urea toxins. Among them, the precursors of protein-bound urea toxins are mostly produced by intestinal microbial metabolism, so they are also called intestinal urea toxins, which have been proven to increase kidney damage and aggravate the process of renal fibrosis.

[0004] Once fibrosis occurs, it is irreversible and CKD will gradually develop into chronic renal failure. The treatment goal of this disease is to take symptomatic supportive therapy to delay further kidney damage. Therefore, dietary management is very important for cats and dogs with CKD. A proper diet can slow down the progression of the disease and prolong life.

[0005] The diet for CKD should meet the following nutritional requirements: moderate protein restriction, low phosphorus, low sodium, high potassium, supplementation of B vitamins, ω-3 fatty acids, alkalizers, etc. In addition to the above nutrient requirements, functional ingredients with antioxidant, anti-inflammatory and anti-fibrosis properties can also be added, such as various natural plant extracts. In addition, the use of probiotics and prebiotics to target intestinal flora to regulate intestinal uremic toxins is also one of the strategies to reduce kidney damage and resist fibrosis.

[0006] Chinese patent CN107519219A discloses a nutritional supplement for pet kidney disease, including essential amino acids, vitamins, trace elements, curcumin, Java tea extract, etc., wherein curcumin has the effects of lowering blood pressure, lowering blood lipids, inhibiting bacteria and inflammation, and anti-oxidation, and Java tea extract can reduce urinary system stones and urinary tract infections. Most of the patents related to food compositions for pet kidney care, represented by Chinese patent CN107519219A, mainly focus on reducing the burden on the kidneys by adjusting the proportion of nutrients, and protecting the urinary system by adding functional ingredients to resist bacteria and inflammation, resist oxidation and reduce stones, but lack the key technical point of anti-fibrosis, and most of them only use plant extracts, with a relatively simple mechanism of action and poor effect. Summary of the invention

[0007] In view of the pathological characteristics of chronic kidney disease in dogs and cats as mentioned above and the blank spots in the existing related technologies, the purpose of the present invention is to provide a functional composition and complete food for kidneys of dogs and cats and a preparation method thereof.

[0008] The specific technical solutions include the following:

[0009] In a first aspect, a functional composition for dog and cat kidneys is provided, comprising a plant extract, a prebiotic and a probiotic.

[0010] Furthermore, the plant extract consists of astragalus extract, rhodiola rosea extract, Centella asiatica extract, Gynostemma pentaphyllum extract and Poria cocos extract.

[0011] Furthermore, the prebiotics consist of fucoidan, inulin and lactulose.

[0012] Furthermore, the probiotics consist of Streptococcus thermophilus, Lactobacillus acidophilus and Bifidobacterium longum.

[0013] Among them, the active ingredients of Astragalus extract, Rhodiola rosea extract, Centella asiatica extract, Gynostemma pentaphyllum extract and Poria cocos extract are mainly polysaccharides and saponins. These active ingredients can reduce renal fibrosis damage by regulating the classical fibrosis pathway, and can also reduce fibrosis by regulating oxidative stress and inflammatory signaling pathways.

[0014] Studies have shown that the number of beneficial bacteria (Helicobacter, Bacillus, Streptococcus, Pseudomonas and Lactobacillus, etc.) in the intestines of CKD animals is reduced, while the number of bacteria that produce uremic toxins (such as Eubacterium mucosum, Oscillococcus and Enteromonas) is increased; supplementation with Streptococcus thermophilus, Lactobacillus acidophilus and Bifidobacterium longum can improve kidney damage.

[0015] Fucoidan, inulin and lactulose as prebiotics can increase the relative abundance of beneficial bacteria such as lactic acid bacteria and bifidobacteria in the intestine, redistribute the excretion pathway of nitrogen in the body, reduce the level of urea toxins, and thus alleviate renal fibrosis.

[0016] Furthermore, the weight percentage of the plant extract in the functional composition for dog and cat kidneys is 16%-85%.

[0017] Furthermore, the weight percentage of the prebiotics in the functional composition for dog and cat kidneys is 0.9%-10%.

[0018] Furthermore, the content of the probiotics in the functional composition for dog and cat kidney is 1×10 11 -8×10 11 CFU / kg.

[0019] In a second aspect, a complete food is provided, comprising the functional composition for dog and cat kidneys described in the first aspect.

[0020] Furthermore, the functional composition for dog and cat kidney accounts for 3-5% by weight of the complete food.

[0021] Preferably, the complete food also includes meat meal, fresh meat, starch, liver, plant fiber, cellulose, mineral elements, antioxidants, chicken oil, and fish oil.

[0022] In a third aspect, a method for preparing the complete food according to the second aspect is provided, comprising the following steps:

[0023] S1. Mixing: Weigh the following components in parts by weight, mix and crush 20-40 parts of meat powder, 10-20 parts of fresh meat, 10-20 parts of starch, 5-8 parts of liver, and 2-8 parts of plant fiber, and then add 1-5 parts of premixed vitamins, 1-3 parts of mineral elements, 0.01-0.2 parts of antioxidants, and plant extracts and prebiotics, and mix them again. The coefficient of variation of the uniformity of the mixture is ≤5%, and a mixed raw material is obtained;

[0024] S2. Puffing: The mixed raw materials are put into the puffing machine at a temperature of 90-130 ° C for 1-5 min for tempering and puffing, and then cut into 4-6 mm particle thickness to obtain cut particles;

[0025] S3 drying: the cut particles at a temperature of 100-130 ℃, time for 30-60min conditions for drying, the weight content of water is controlled below 8%, to obtain dried particles;

[0026] S4. Cooling and spraying: The dried particles are placed in a cooling box and cooled by a cold air blower. When the temperature drops to 50-80 ° C, 2-8 parts by weight of chicken fat and 1-3 parts by weight of fish oil are sprayed by a liquid spraying device; continue to cool to a temperature difference of ≤5 ° C with the room temperature, and the probiotics are evenly sprayed on the surface of the particles with a powder spraying device to obtain sprayed particles;

[0027] S5. Packaging: The sprayed particles are sieved and impurities are removed before packaging to obtain complete food.

[0028] Compared with the prior art, the functional composition and complete food for dog and cat kidneys provided by the present invention and the preparation method thereof have the following beneficial effects:

[0029] The functional composition for dog and cat kidneys is selected from plant extracts, prebiotics and probiotics, and has anti-oxidation, anti-inflammatory and anti-fibrosis effects on dog and cat kidneys. The complete food including the functional composition for dog and cat kidneys is applied to pet food, which can prevent dog and cat kidney damage or delay the disease progression of dog and cat chronic kidney disease. Compared with existing pet functional foods, the present invention provides a dietary solution that provides kidney care from the perspective of anti-fibrosis. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.

[0032] It should also be understood that the terms used in this specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. As used in the specification of the present invention and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.

[0033] It should be further understood that the term "and / or" used in the present description and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes these combinations.

[0034] In order to more fully understand the technical content of the present invention, the technical solution of the present invention is further introduced and illustrated in conjunction with specific embodiments below.

[0035] Example 1

[0036] A functional composition for dog and cat kidneys, comprising 16.65% by weight of plant extracts (consisting of 3.33% astragalus extract, 3.33% rhodiola rosea extract, 3.33% centella asiatica extract, 3.33% gynostemma pentaphyllum extract and 3.33% tuckahoe extract), 0.99% by weight of prebiotics (consisting of 0.33% fucoidan, 0.33% inulin and 0.33% lactulose) and 1×10 probiotics. 11 CFU / kg (3.33×10 10 CFU / kg, Lactobacillus acidophilus 3.33×10 10 CFU / kg and Bifidobacterium longum 3.33×10 10 CFU / kg composition).

[0037] Example 2

[0038] A functional composition for dog and cat kidneys, comprising 83.35% by weight of plant extracts (consisting of 16.67% astragalus extract, 16.67% rhodiola rosea extract, 16.67% centella asiatica extract, 16.67% gynostemma pentaphyllum extract and 16.67% tuckahoe extract), 9.99% by weight of prebiotics (consisting of 3.33% fucoidan, 3.33% inulin and 3.33% lactulose) and 8×10 probiotics. 11 CFU / kg (26.67×10 10 CFU / kg, Lactobacillus acidophilus 26.67×10 10 CFU / kg and Bifidobacterium longum 26.67×10 10 CFU / kg composition).

[0039] Example 3

[0040] A functional composition for dog and cat kidneys, comprising 50% by weight of plant extract (consisting of 10% astragalus extract, 10% rhodiola rosea extract, 10% centella asiatica extract, 10% gynostemma pentaphyllum extract and 10% tuckahoe extract), 5.49% by weight of prebiotics (consisting of 1.83% fucoidan, 1.83% inulin and 1.83% lactulose) and 4.5×10 11 CFU / kg (15×10 10 CFU / kg, Lactobacillus acidophilus 15×10 10 CFU / kg and Bifidobacterium longum 15×1010 CFU / kg composition).

[0041] Comparative Example 1

[0042] The difference between Control Example 1 and Example 3 is that the functional composition for kidney of dogs and cats does not contain prebiotics and probiotics, and the other conditions are the same.

[0043] Comparative Example 2

[0044] The difference between Control Example 2 and Example 3 is that the functional composition for kidney of dogs and cats does not contain plant extracts and probiotics, and the other conditions are the same.

[0045] Comparative Example 3

[0046] The difference between Control Example 3 and Example 3 is that the functional composition for kidney of dogs and cats does not contain plant extracts and prebiotics, and the other conditions are the same.

[0047] Comparative Example 4

[0048] The difference between Control Example 4 and Example 3 is that the functional composition for kidney of dogs and cats does not contain probiotics, and the other conditions are the same.

[0049] Comparative Example 5

[0050] The difference between Control Example 5 and Example 3 is that the functional composition for kidney of dogs and cats does not contain prebiotics, and the other conditions are the same.

[0051] Comparative Example 6

[0052] The difference between Control Example 6 and Example 3 is that the functional composition for dog and cat kidney does not contain plant extracts, and the other conditions are the same.

[0053] Comparative Example 7

[0054] A functional composition for dog and cat kidneys, comprising 5% by weight of plant extract (consisting of 1% astragalus extract, 1% rhodiola rosea extract, 1% centella asiatica extract, 1% gynostemma pentaphyllum extract and 1% tuckahoe extract), 5.49% by weight of prebiotics (consisting of 1.83% fucoidan, 1.83% inulin and 1.83% lactulose) and 4.5×10 11 CFU / kg (15×10 10 CFU / kg, Lactobacillus acidophilus 15×10 10 CFU / kg and Bifidobacterium longum 15×10 10 CFU / kg composition).

[0055] Comparative Example 8

[0056] A functional composition for dog and cat kidneys, comprising 50% by weight of plant extract (consisting of 10% astragalus extract, 10% rhodiola rosea extract, 10% centella asiatica extract, 10% gynostemma pentaphyllum extract and 10% tuckahoe extract), 0.3% by weight of prebiotics (consisting of 0.1% fucoidan, 0.1% inulin and 0.1% lactulose) and 4.5×10 probiotics. 11 CFU / kg (15×10 10 CFU / kg, Lactobacillus acidophilus 15×10 10 CFU / kg and Bifidobacterium longum 15×10 10 CFU / kg composition).

[0057] Comparative Example 9

[0058] A functional composition for dog and cat kidneys, comprising 50% by weight of plant extract (consisting of 10% astragalus extract, 10% rhodiola rosea extract, 10% centella asiatica extract, 10% gynostemma pentaphyllum extract and 10% tuckahoe extract), 5.49% by weight of prebiotics (consisting of 1.83% fucoidan, 1.83% inulin and 1.83% lactulose) and 3×10 probiotics. 10 CFU / kg (1×10 10 CFU / kg, Lactobacillus acidophilus 1×10 10 CFU / kg and Bifidobacterium longum 1×10 10 CFU / kg composition).

[0059] Comparative Example 10

[0060] A functional composition for dog and cat kidneys, comprising 90% by weight of plant extracts (consisting of 18% astragalus extract, 18% rhodiola rosea extract, 18% centella asiatica extract, 18% gynostemma pentaphyllum extract and 18% tuckahoe extract), 5.49% by weight of prebiotics (consisting of 1.83% fucoidan, 1.83% inulin and 1.83% lactulose) and 4.5×10 11 CFU / kg (15×10 10 CFU / kg, Lactobacillus acidophilus 15×10 10 CFU / kg and Bifidobacterium longum 15×10 10 CFU / kg composition).

[0061] Comparative Example 11

[0062] A functional composition for dog and cat kidneys, comprising 50% by weight of plant extract (consisting of 10% astragalus extract, 10% rhodiola rosea extract, 10% centella asiatica extract, 10% gynostemma pentaphyllum extract and 10% tuckahoe extract), 15% by weight of prebiotics (consisting of 5% fucoidan, 5% inulin and 5% lactulose) and 4.5×10 probiotics. 11 CFU / kg (15×10 10 CFU / kg, Lactobacillus acidophilus 15×10 10 CFU / kg and Bifidobacterium longum 15×10 10 CFU / kg composition).

[0063] Comparative Example 12

[0064] A functional composition for dog and cat kidneys, comprising 50% by weight of plant extract (consisting of 10% astragalus extract, 10% rhodiola rosea extract, 10% centella asiatica extract, 10% gynostemma pentaphyllum extract and 10% tuckahoe extract), 5.49% by weight of prebiotics (consisting of 1.83% fucoidan, 1.83% inulin and 1.83% lactulose) and 1.5×10 12 CFU / kg (50×10 10 CFU / kg, Lactobacillus acidophilus 50×10 10 CFU / kg and Bifidobacterium longum 50×10 10 CFU / kg composition).

[0065] Test Example 1

[0066] A complete food comprises the functional composition for dog and cat kidneys of embodiment 1, wherein the functional composition for dog and cat kidneys accounts for 3% of the weight of the complete food.

[0067] The method for preparing the complete food comprises the following steps:

[0068] S1. Mixing: Weigh the following components by weight, mix and crush 30 parts of meat powder, 15 parts of fresh meat, 15 parts of starch, 6 parts of liver, and 5 parts of plant fiber, then add 3 parts of premixed vitamins, 2 parts of mineral elements, 0.1 parts of antioxidants, and plant extracts and prebiotics, and mix them again. The coefficient of variation of the uniformity of the mixture is ≤5%, and a mixed raw material is obtained;

[0069] S2. Puffing: The mixed raw materials were put into the puffing machine at a temperature of 110 ° C for 3 minutes for tempering and puffing, and then cut into 5 mm particles to obtain cut particles;

[0070] S3 drying: the cut particles were dried at a temperature of 115 ° C for 45 min, the weight content of water was controlled below 8%, to obtain dried particles;

[0071] S4. Cooling and spraying: The dried particles were placed in a cooling box and cooled by a cold air blower. When the temperature dropped to 65 ° C, 5 parts by weight of chicken fat and 2 parts by weight of fish oil were sprayed by a liquid spraying device; when the temperature difference with the room temperature was ≤5 ° C, the probiotics were evenly sprayed on the surface of the particles with a powder spraying device to obtain sprayed particles;

[0072] S5. Packaging: The sprayed particles are sieved and impurities are removed before packaging to obtain complete food.

[0073] Test Example 2

[0074] The difference between Test Example 2 and Test Example 1 is that the functional composition for dog and cat kidney of Example 2 is used to replace the functional composition for dog and cat kidney of Example 1 in Test Example 1, and the other conditions are the same.

[0075] Test Example 3

[0076] The difference between Test Example 3 and Test Example 1 is that the functional composition for dog and cat kidney of Example 3 is used to replace the functional composition for dog and cat kidney of Example 1 in Test Example 1, and the other conditions are the same.

[0077] Comparative Example 1

[0078] The difference between Comparative Example 1 and Experimental Example 1 is that the complete food does not contain the functional composition for kidneys of dogs and cats, and the other conditions are the same.

[0079] Performance Testing - Animal Experimental Protocol:

[0080] 1. Experimental results in mice

[0081] 1.1 Experimental Materials and Methods

[0082] 170 SPF male SD rats weighing 200±20 g were selected and randomly divided into 17 groups after 7 days of adaptive feeding, namely, a blank group, a CKD model group, Example 1 to 3 groups, and Control 1 to 12 groups. The experimental period was 28 days.

[0083] The specific experimental method is as follows: the blank group was gavaged with distilled water 300 mg / kg / d daily, the model group, the embodiment group and the control group were gavaged with adenine suspension 300 mg / kg / d daily, the blank group and the model group were fed with basic mouse food daily, and the embodiment group and the control group were fed with basic mouse food to which the functional composition for dog and cat kidney of the above-mentioned embodiment and the control group was added (converted to rat dosage according to body surface area, wherein the preparation method of the functional composition for dog and cat kidney of the embodiment and the control group is prepared by mixing plant extracts, prebiotics and probiotics), for 28 consecutive days. The gavage and feeding conditions of the blank group, the model group, the embodiment group and the control group are shown in Table 1 below:

[0084] Table 1. Stomach gavage and feeding of blank group, model group, embodiment group and control group

[0085] Grouping Oral gavage Feeding Blank Group Distilled water 300 mg / kg / day Basic rat food 180g / kg / d Model Group Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d Example 1 Group Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d+Example 1 Example 2 Group Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d+Example 2 Example 3 Group Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d+Example 3 Control group 1 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 1 Control group 2 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 2 Control group 3 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 3 Control group 4 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 4 Control group 5 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 5 Control group 6 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 6 Control group 7 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 7 Control group 8 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 8 Control group 9 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 9 Control group 10 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 10 Control group 11 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 11 Control group 12 Adenine suspension 300 mg / kg / d Basic rat food 180g / kg / d + control example 12

[0086] On the 29th day, blood was collected through the eye sockets to detect the levels of plasma creatinine (SCR) and blood urea nitrogen (BUN) in rats. Then the rats were killed, and the kidneys were removed to detect the contents of malondialdehyde (MDA), superoxide dismutase (SOD), interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), fibronectin (FN), and transforming growth factor β1 (TGF-β1) in kidney tissues.

[0087] 1.2 Experimental Results and Analysis

[0088] Table 2 Test results of rat plasma renal function indexes ( n=10)

[0089] Group SCR (μmol / L) BUN (mmol / L) Blank Group <![CDATA[45.26±15.12 c ]]> <![CDATA[6.51±0.98 c ]]> Model Group <![CDATA[356.19±71.45 a ]]> <![CDATA[31.68±4.08 a ]]> Example 1 Group <![CDATA[241.93±76.11 b ]]> <![CDATA[23.17±3.87 b ]]> Example 2 Group <![CDATA[235.47±62.84 b ]]> <![CDATA[21.12±3.57 b <!-- 6 -->]]> Example 3 Group <![CDATA[239.33±64.12 b ]]> <![CDATA[22.49±4.41 b ]]> Control group 1 <![CDATA[310.27±70.76 ab ]]> <![CDATA[28.12±4.83 ab ]]> Control group 2 <![CDATA[302.45±72.39 ab ]]> <![CDATA[27.18±5.13 ab ]]> Control group 3 <![CDATA[306.86±69.52 ab ]]> <![CDATA[28.09±4.28 ab ]]> Control group 4 <![CDATA[289.54±69.39 ab ]]> <![CDATA[26.62±4.18 ab ]]> Control group 5 <![CDATA[291.24±78.31 ab ]]> <![CDATA[27.03±5.04 ab ]]> Control group 6 <![CDATA[296.32±70.23 ab ]]> <![CDATA[26.15±4.87 ab ]]> Control group 7 <![CDATA[271.93±62.58 ab ]]> <![CDATA[25.19±4.05 ab ]]> Control group 8 <![CDATA[269.37±69.39 ab ]]> <![CDATA[26.83±4.69 ab ]]> Control group 9 <![CDATA[275.23±62.35 ab ]]> <![CDATA[25.75±5.09 ab ]]> Control group 10 <![CDATA[276.14±76.38 ab ]]> <![CDATA[25.24±5.32 ab ]]> Control group 11 <![CDATA[281.63±78.38 ab ]]> <![CDATA[26.12±4.21 ab ]]> Control group 12 <![CDATA[279.17±74.26 ab ]]> <![CDATA[26.03±4.59 ab ]]>

[0090] From the plasma renal function indexes in Table 2, it can be seen that compared with the blank group, the creatinine and urea nitrogen in the model group increased significantly, indicating that the modeling was successful; compared with the model group, the creatinine and urea nitrogen in the Example 1-3 groups decreased significantly, indicating that the dosages of the three example compositions all have a protective effect on the kidneys and can reduce kidney damage, and there is no significant difference between the examples. The creatinine and urea nitrogen in the reference examples 1-6 groups are not significantly different from those in the model group, indicating that at the same dose level, the functional composition for the kidneys of dogs and cats in the reference examples 1-6 is significantly lower than the functional composition for the kidneys of dogs and cats in Example 3 for the protection of the kidneys, and the reference examples 1-3 groups lacking multiple components are worse than the reference examples 4-6 groups lacking a single component; the creatinine and urea nitrogen in the reference examples 7-12 are not significantly different from those in the model group, indicating that the protective effect of the functional composition for the kidneys of dogs and cats in the reference examples 7-12 is significantly lower than the composition within the dosage range described in the examples.

[0091] Table 3 Test results of oxidative stress, inflammation and fibrosis indexes in rat kidney ( n=10)

[0092]

[0093] Table 3 shows the results of kidney tissue detection, including oxidative stress indicators MDA and SOD, inflammation indicators IL-6 and TNF-α, and fibrosis indicators FN and TGF-β1. Compared with the blank group, the MDA of the model group increased significantly, SOD decreased significantly, IL-6 and TNF-α increased significantly, and FN and TGF-β1 also increased significantly, indicating that the modeling was successful. Compared with the model group, the MDA of the embodiment group decreased significantly, the SOD activity increased significantly, IL-6 and TNF-α decreased significantly, and FN and TGF-β1 also decreased significantly, indicating that the three embodiments all play an antioxidant, anti-inflammatory and anti-fibrotic protective role on the kidney, can reduce kidney damage, and there is no significant difference between the embodiments. There were no significant differences in MDA, SOD and TNF-α between the control group 1-12 and the model group. Although IL-6, FN and TGF-β1 were significantly reduced compared with the model group, they were also significantly higher than those of the example 1-3 groups, indicating that the functional composition for canine and cat kidney in the control example had a significantly lower renal protective effect than the functional composition for canine and cat kidney in the example.

[0094] 2. Cat Experiment Effect

[0095] 2.1 Experimental Materials and Methods

[0096] 16 adult Chinese rural cats with kidney indicators higher than normal were screened at the stray cat base, half of them were of each sex and weighed 3.2-4.5 kg. After 7 days of adaptive feeding, they were randomly divided into 4 groups, namely, the control group, test example group 1, test example group 2 and test example group 3. The control group was fed with the basic diet (complete food) of comparative example 1, and the test example group was fed with the diet (complete food) of test examples 1, 2 and 3 respectively, for 28 days, during which they could freely eat and drink. On the 29th day, blood was collected through the head vein to detect plasma SCR, BUN, MDA, SOD, IL-6, TNF-α and matrix metalloproteinase 7 (MMP-7).

[0097] 2.2 Experimental Results and Analysis

[0098] Table 4 Cat plasma renal function index test results ( n=4)

[0099] Group SCR (μmol / L) BUN (mmol / L) Comparison group 225.29±30.52 24.13±4.26 Test Example 1 <![CDATA[165.47±25.32 a ]]> <![CDATA[16.56±3.28 a ]]> Test Example 2 <![CDATA[171.14±23.73 a ]]> <![CDATA[17.02±3.03 a ]]> Test Example 3 Group <![CDATA[159.93±26.27 a ]]> <![CDATA[16.18±4.22 a ]]>

[0100] Note: Compared with the control group, a P<0.05.

[0101] As can be seen from Table 4, compared with the control group, the creatinine and urea nitrogen of the test group were significantly reduced, indicating that the three test examples all have a protective effect on the kidneys and can reduce kidney damage, and there are no significant differences between the tests.

[0102] Table 5 Results of cat plasma oxidative stress, inflammation and fibrosis index detection ( n=4)

[0103]

[0104]

[0105] Note: Compared with the control group, a P<0.05.

[0106] There is currently no specific plasma biomarker for detecting fibrosis. MMP-7 is highly positively correlated with the degree of fibrosis and can reflect the level of fibrosis to a certain extent. As shown in Table 5, compared with the control group, the MDA of the test group decreased significantly, SOD increased significantly, IL-6 and TNF-α decreased significantly, and MMP-7 also decreased significantly, indicating that the three test cases have an overall antioxidant, anti-inflammatory and anti-fibrotic protective effect on cats with abnormal kidney health, and there is no significant difference between the several test cases.

[0107] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the present invention, and these modifications or replacements should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. A functional composition for dog and cat kidneys, characterized in that: Includes plant extracts, prebiotics and probiotics.

2. The functional composition for dog and cat kidneys according to claim 1, characterized in that: The plant extract consists of astragalus extract, rhodiola rosea extract, centella asiatica extract, gynostemma pentaphyllum extract and tuckahoe extract.

3. The functional composition for dog and cat kidneys according to claim 2, characterized in that: The prebiotics consist of fucoidan, inulin and lactulose.

4. The functional composition for dog and cat kidneys according to claim 3, characterized in that: The probiotics consist of thermophilic streptococcus, lactobacillus acidophilus and bifidobacterium longum.

5. The functional composition for dog and cat kidneys according to claim 1, characterized in that: The weight percentage of the plant extract in the functional composition for dog and cat kidney is 16%-85%.

6. The functional composition for dog and cat kidneys according to claim 5, characterized in that: The weight percentage of the prebiotics in the functional composition for kidneys of dogs and cats is 0.9%-10%.

7. The functional composition for dog and cat kidneys according to claim 6, characterized in that: The content of the probiotics in the functional composition for dog and cat kidney is 1×10 11 -8×10 11 CFU / kg.

8. A complete food, characterized in that: A functional composition for dog and cat kidneys comprising the composition described in any one of claims 1 to 7.

9. The complete food according to claim 6, characterized in that The weight percentage of the functional composition for dog and cat kidneys is 3-5% of the complete food.

10. The method for preparing a complete food according to any one of claims 8 to 9, characterized in that: The following steps are involved: S1. Mixing: Weigh the following components in parts by weight, mix and crush 20-40 parts of meat powder, 10-20 parts of fresh meat, 10-20 parts of starch, 5-8 parts of liver, and 2-8 parts of plant fiber, and then add 1-5 parts of premixed vitamins, 1-3 parts of mineral elements, 0.01-0.2 parts of antioxidants, and plant extracts and prebiotics, and mix them again. The coefficient of variation of the uniformity of the mixture is ≤5%, and a mixed raw material is obtained; S2. Puffing: The mixed raw materials are put into the puffing machine at a temperature of 90-130 ° C for 1-5 min for tempering and puffing, and then cut into 4-6 mm particle thickness to obtain cut particles; S3 drying: the cut particles at a temperature of 100-130 ℃, time for 30-60min conditions for drying, the weight content of water is controlled below 8%, to obtain dried particles; S4. Cooling and spraying: The dried particles are placed in a cooling box and cooled by a cold air blower. When the temperature drops to 50-80 ° C, 2-8 parts by weight of chicken fat and 1-3 parts by weight of fish oil are sprayed by a liquid spraying device; continue to cool to a temperature difference of ≤5 ° C with the room temperature, and the probiotics are evenly sprayed on the surface of the particles with a powder spraying device to obtain sprayed particles; S5. Packaging: The sprayed particles are sieved and impurities are removed before packaging to obtain complete food.

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