Automatic blood sampling device for laboratory animal rats and mice
By designing an automatic blood collection device, using a carbon dioxide mask and an infrared sensing device to achieve rapid unconscious state and precise blood collection, the problem of complex and inefficient blood collection methods for large and small mice in the prior art is solved, and blood collection efficiency and work efficiency are significantly improved.
Patent Information
- Application Number
- CN202411609315.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2024-12-31
AI Technical Summary
In the prior art, the blood collection method for cardiac blood collection of mice is complicated and inefficient, resulting in high damage to animals and low working efficiency, especially in small experimental animals, which is difficult to achieve efficient blood collection.
An automatic blood collection device for mice with large and small experimental animals was designed, using a carbon dioxide mask to quickly make the experimental mice enter an unconscious state, and precise blood collection is achieved through infrared sensing devices and automatic collection needles of the robotic arm, and multiple simultaneous collection is achieved by combining the driving components.
The blood collection efficiency of mice of all sizes has been greatly improved, the damage to animals is reduced, and the work efficiency has been improved. The number of rats that can be collected in a single person in a day has increased from 160 per day to 860 per day (mouse) and 440 per day. (Rat).
Smart Images
Figure CN119214818A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of blood sampling devices for experimental animals, and in particular to an automatic blood sampling device for experimental animals, rats and mice. Background Art
[0002] Blood products are basic reagents for many biological experiments. In the validation of biological sample analysis methodology (PK experiments, mass spectrometry analysis, etc.), a large amount of blood blank matrix controls or blood dilution of samples are required. In experiments related to in vitro cell culture, they can replace other animal sera to provide basic nutrients or nutrients lacking in synthetic culture media. They can be used for animal immunization and serum IgG preparation. In pathological histochemical blocking and immune experiments such as ELISA, they can act as blocking and protective agents.
[0003] There are various ways to collect blood from mice and rats, including tail vein blood collection, retro-orbital venous plexus blood collection, submandibular venous plexus blood collection, abdominal aorta blood collection, and cardiac blood collection. Among them, cardiac blood collection is used by most experimenters because of its large collection volume and relative sterility. However, this method is complicated to operate. Compared with other blood collection methods, cardiac blood collection requires higher operating skills and experience, especially on small experimental animals such as mice, which is more difficult.
[0004] The above method is complicated to collect blood, and cardiac blood collection is very harmful to animals. Therefore, it is generally used for terminal blood collection in the existing technology. Specifically, before collection, a special person needs to euthanize the animal individually for 5-11 minutes (the euthanasia time is related to the number of animals); then the mouse is handed over to the technician for disinfection and collection. The disinfection + collection time is about 3 minutes for mice and 4 minutes for rats; the euthanasia time is integrated with the collection time. Taking mice and rats as an example, rats: 8 minutes for each euthanasia, 2 rats are collected each time; 8 working hours a day, the work efficiency is equal to 120 rats / person / day. Each euthanasia is 6 minutes, and 2 rats are collected each time; 8 working hours a day, the work efficiency is equal to 160 rats / person / day. However, the collection efficiency of this single transfer method is low, and there is room for further improvement. Summary of the invention
[0005] In order to improve the blood collection efficiency of mice and rats, the present application provides an automatic blood collection device for experimental animals, mice and rats.
[0006] The automatic blood sampling device for experimental animals and mice provided in this application adopts the following technical solution: An automatic blood sampling device for experimental animals, mice and rats, comprises a base plate, a support column is fixedly connected to the base plate, a turntable is rotatably connected to the support column, a plurality of fixing components for fixing experimental mice on the end surface of the turntable are installed on the turntable, a plurality of the fixing components are arranged around the axis of the turntable and are placed at the edge of the turntable, an automatic blood collection needle for collecting blood from the experimental mice on the turntable is provided on one side of the base plate, a control component for controlling the automatic blood collection needle to collect blood is provided on one side of the base plate, a plurality of carbon dioxide masks are provided on the turntable, a gas supply component for conveying carbon dioxide into the carbon dioxide masks is provided on the base plate, and a driving component for driving the turntable to rotate is provided on the support column.
[0007] By adopting the above technical solution, the experimental mice can be placed on the turntable in turn, and the experimental mice are fixed on the turntable by the fixing component, and then the carbon dioxide mask is put on the head of the experimental mouse, and then the carbon dioxide is delivered into the carbon dioxide mask by the air supply component, so that the experimental mouse quickly enters an unconscious state within 30 seconds, and then the turntable is controlled to rotate by the driving component, and the control component drives the automatic collection needle to collect the experimental mice fixed on the turntable in turn. In this way, multiple experimental mice can be collected at the same time, which greatly improves the blood collection efficiency of large and small experimental mice.
[0008] Optionally, the fixing assembly includes an underarm fixing clip, a head and neck fixing clip is provided on one side of the underarm fixing clip close to the center of the turntable, and both the underarm fixing clip and the head and neck fixing clip are installed on the turntable.
[0009] By adopting the above technical solution, two fixing clips can be set. The armpit fixing clip is larger to fix the armpit to prevent the animal from escaping, and the head and neck fixing clip is smaller and denser to fix the skin of the back of the head and neck of the animal to prevent the animal's head from swinging. In this way, the experimental mouse can be stably fixed on the turntable.
[0010] Optionally, the control component includes a robotic arm mounted on the base plate, an infrared sensing device is mounted on the upper end of the robotic arm, the infrared sensing device is electrically connected to the robotic arm and the automatic collection needle, and the automatic collection needle is fixedly connected to the upper end of the robotic arm.
[0011] By adopting the above technical solution, an infrared sensing device is provided on the top of the robotic arm to sense the infrared radiation emitted by the heart, thereby quickly determining the position of the experimental mouse. The automatic collection needle device on the robotic arm can accurately insert the automatic collection needle into the body of the experimental mouse based on the infrared recognition of the infrared sensing device.
[0012] Optionally, the gas supply assembly includes a carbon dioxide gas storage tank, and a plurality of gas supply pipes are fixedly connected to and communicated with the upper portion of the carbon dioxide gas storage tank. The gas supply pipes correspond one-to-one to the carbon dioxide masks and are communicated with the carbon dioxide masks.
[0013] By adopting the above technical solution, carbon dioxide can be stored in a carbon dioxide gas storage tank and transported to each carbon dioxide mask through an air supply pipe.
[0014] Optionally, a cavity is defined in the support column, and the carbon dioxide storage tank is fixedly connected in the cavity.
[0015] By adopting the above technical solution, the area occupied by the carbon dioxide gas storage tank can be reduced by arranging the carbon dioxide gas storage tank in the cavity, thereby reducing the occupied area of the device.
[0016] Optionally, a rotating head is installed at the upper end of the support column, and the rotating holes on the rotating head correspond one-to-one with the air supply pipe and are fixedly connected to the air supply pipe.
[0017] By adopting the above technical solution, multiple air supply pipes can be integrated through a rotating head so that they are distributed more regularly on the upper end of the turntable, thereby reducing the occurrence of mutual entanglement.
[0018] Optionally, a boss is provided at the upper end surface of the turntable, the boss is arranged in an annular shape and is located close to a side of the inner arc surface of the turntable, and the carbon dioxide mask is placed on a side of the boss away from the center of the turntable.
[0019] By adopting the above technical solution, the position of carbon dioxide on the turntable can be limited by setting a boss, thereby reducing the possibility of carbon dioxide passing through the center of the turntable and falling below the turntable.
[0020] Optionally, a soft silicone rubber for inserting into the head of an experimental mouse is fixedly connected to the inner wall of the carbon dioxide mask, and the soft silicone rubber is arranged in a ring shape and surrounds the inner wall of the carbon dioxide mask.
[0021] By adopting the above technical solution, the position of the carbon dioxide mask on the head of the test mouse can be limited by soft silicone, thereby improving the sealing performance of the carbon dioxide mask.
[0022] Optionally, the driving assembly includes a passive bevel gear ring fixedly connected to the lower end of the turntable, the passive bevel gear ring is coaxially arranged with the turntable, an active bevel gear is meshed below the passive bevel gear ring, a driving motor is fixedly connected to the support column, and the output shaft of the driving motor is fixedly connected to the active bevel gear.
[0023] By adopting the above technical solution, the driving motor can be controlled to rotate, so that the driving motor drives the active bevel gear to rotate, and then the active bevel gear drives the passive bevel gear ring to rotate, and then the turntable rotates.
[0024] In summary, the present application includes at least one of the following beneficial technical effects: 1. Carbon dioxide can be delivered to the carbon dioxide mask through the air supply component, so that the experimental mouse quickly enters an unconscious state within 30 seconds, and then the turntable is controlled by the driving component to rotate. At the same time, the control component will drive the automatic collection needle to collect the experimental mice fixed on the turntable in turn. In this way, multiple experimental mice can be collected at the same time, greatly improving the blood collection efficiency of large and small experimental mice; 2. An infrared sensing device is installed on the top of the robotic arm to sense the infrared radiation emitted by the heart, thereby quickly determining the position of the experimental mouse. The automatic collection needle on the robotic arm can accurately insert the automatic collection needle into the body of the experimental mouse based on the infrared recognition of the infrared sensing device; 3. The fixing clips can be set to two. The armpit fixing clip is larger to fix the armpit to prevent the animal from escaping, and the head and neck fixing clip is small and dense to fix the skin of the back of the head and neck of the animal to prevent the animal's head from swinging. In this way, the experimental mouse can be stably fixed on the turntable. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application; Figure 2 yes Figure 1 A partial enlarged schematic diagram of part A; Figure 3 It is a schematic diagram of the explosion structure of the cavity in the embodiment of the present application; Figure 4 It is a schematic diagram of the structure of the driving component of an embodiment of the present application.
[0026] In the figure, 1. base plate; 2. support column; 21. cavity; 3. fixing component; 31. underarm fixing clip; 32. head and neck fixing clip; 4. automatic collection needle; 5. control component; 51. mechanical arm; 52. infrared sensing device; 6. carbon dioxide mask; 7. gas supply component; 71. carbon dioxide gas storage tank; 72. gas supply pipe; 8. drive component; 81. drive motor; 82. passive bevel gear ring; 83. active bevel gear; 84. control button; 9. rotating head; 10. turntable; 101. boss; 11. soft silicone; 12. collection box. DETAILED DESCRIPTION
[0027] The following is combined with Figure 1 -Attached Figure 4 , further details of this application are given.
[0028] The embodiment of the present application is: an automatic blood collection device for experimental animals, rats and mice, referring to Figure 1 and Figure 2 , comprising a bottom plate 1, which is arranged in a rectangular parallelepiped shape. A support column 2 is fixedly connected to the bottom plate 1, and the support column 2 is arranged perpendicular to the upper end surface of the bottom plate 1. A turntable 10 is rotatably connected to the support column 2, and the end surface of the turntable 10 is arranged parallel to the bottom plate 1. The turntable 10 is equipped with a plurality of fixing components 3 for fixing the experimental mice on the end surface of the turntable 10.
[0029] A plurality of groups of fixing components 3 are arranged equidistantly around the axis of the rotating disk 10 and are placed at the edge of the rotating disk 10. In this embodiment, the fixing components 3 are arranged in 10 groups.
[0030] The fixing assembly 3 includes an underarm fixing clip 31, and a head and neck fixing clip 32 is provided on one side of the underarm fixing clip 31 close to the center of the turntable 10. The underarm fixing clip 31 and the head and neck fixing clip 32 are both installed on the turntable 10. The underarm fixing clip 31 and the head and neck fixing clip 32 are both configured as electronic claw clips and are both equipped with infrared emitting tubes, which are not shown in the figure. The infrared emitting tube is electrically connected to the underarm fixing clip 31 and the head and neck fixing clip 32, thereby enabling the underarm fixing clip 31 and the head and neck fixing clip 32 to have an infrared recognition function, wherein the underarm fixing clip 31 and the head and neck fixing clip 32 are usually in an open state, and the infrared rays are blocked by the animal before the animal is caught, so as to avoid the situation of catching the mouse with one hand and looking for the switch with the other hand, and the grabbing is more stable and convenient; the grabbing time of a mouse is 325s-330s, and the grabbing time of a rat is 9 minutes 30s-10 minutes.
[0031] On one side of the bottom plate 1, there is an automatic blood collection needle 4 for collecting blood from the laboratory mice on the turntable 10. The automatic blood collection needle 4 is a blood collection needle for collecting animal blood in the prior art. On the bottom plate 1, there is a collection box 12 for collecting dead laboratory mice after the collection is completed. The collection box 12 is set in a rectangular parallelepiped shape and its upper end is open. On one side of the bottom plate 1, there is a control component 5 for controlling the automatic blood collection needle 4 to collect blood.
[0032] The control component 5 includes a mechanical arm 51, and an infrared sensing device 52 is installed at the upper end of the mechanical arm 51. The infrared sensing device 52 is electrically connected to the mechanical arm 51 and the automatic collection needle 4, and the automatic collection needle 4 is fixedly connected to the upper end of the mechanical arm 51. Infrared technology can be used to sense the infrared radiation emitted by the target to achieve accurate identification of the target. The heart of the experimental mouse is a blood pumping organ. The strongest point of the heart beat is usually located in the apex area. The left and right ventricles in the apex area have rich blood flow, high temperature and regular beating, and the infrared radiation emitted is the strongest; the infrared radiation characteristics are used to accurately detect the position, temperature and depth of the animal's heart ventricle. The mechanical arm 51 can automatically grab and drive the automatic collection needle 4 to move, and according to the infrared detection position, the collection needle is accurately inserted into the ventricle, making the entire collection process more accurate and faster. At the same time, infrared irradiation has a broad-spectrum bactericidal effect, which can play an anti-inflammatory and bactericidal role on the irradiated part; at the same time, it can promote local blood circulation to be more abundant.
[0033] The turntable 10 is provided with a plurality of carbon dioxide masks 6. In this embodiment, the number of carbon dioxide masks 6 is ten and corresponds to the fixed component 3 one by one. The carbon dioxide mask 6 is made of PP material, which can prevent biting and facilitate high pressure and cleaning; the mask is semi-elliptical in shape, with sufficient space for comfort, and a circle of soft silicone 11 is tightly connected to the bottom. The inner layer of silicone is in a scattered shape, which can be directly put into the animal's head, and has excellent sealing performance, thereby making the carbon dioxide utilization rate high and the euthanasia speed faster. The experimental mouse can enter an unconscious state in 30 seconds, while reducing the diffusion rate of carbon dioxide and saving carbon dioxide; because mice and rats are red-green color blind, they see red as black, so the mask color is red. It can calm the animals and provide a sufficient sense of security.
[0034] Reference Figure 1 , Figure 3 and Figure 4 The bottom plate 1 is provided with an air supply assembly 7 for delivering carbon dioxide to the carbon dioxide mask 6. The gas supply assembly 7 includes a carbon dioxide gas storage tank, a cavity 21 is provided in the support column 2, and the carbon dioxide gas storage tank is fixedly connected in the cavity 21. A plurality of gas supply pipes 72 are fixedly connected and communicated with the upper portion of the carbon dioxide gas storage tank 71, and the gas supply pipes 72 correspond to and communicate with the carbon dioxide mask 6 one by one. At the same time, in order to reduce the situation where the plurality of gas supply pipes 72 are entangled with each other, a rotating head 9 is installed at the upper end of the support column 2, and the rotating head 9 is set as a ten-hole rotating head 9. The rotating holes on the rotating head 9 correspond to and are fixedly connected with the gas supply pipes 72 one by one.
[0035] At the same time, in order to limit the position of the rotating head 9 on the turntable 10, a boss 101 is provided on the upper end surface of the turntable 10. The boss 101 is arranged as an annular boss 101 coaxially with the turntable 10. The boss 101 is located close to the inner arc surface of the turntable 10, and the carbon dioxide mask 6 is placed on the side of the boss 101 away from the center of the turntable 10.
[0036] The support column 2 is provided with a driving assembly 8 for driving the turntable 10 to rotate. The driving assembly 8 includes a passive bevel gear ring 82 fixedly connected to the lower end of the turntable 10, the passive bevel gear ring 82 is coaxially arranged with the turntable 10, and an active bevel gear 83 is meshed below the passive bevel gear ring 82. A driving motor 81 is fixedly connected to the support column 2, and the output shaft of the driving motor 81 is parallel to the length direction of the support column 2. The output shaft of the driving motor 81 is coaxially arranged and fixedly connected with the active bevel gear 83. A control button 84 is installed on the support column 2, and the control button 84 is electrically connected to the driving motor 81 and is used to control the driving motor 81 to rotate.
[0037] By controlling the driving motor 81 to rotate, the driving motor 81 drives the active bevel gear 83 to rotate, and then the active bevel gear 83 drives the passive bevel gear ring 82 to rotate, and then the turntable 10 rotates. And the speed can be adjusted according to the size of the collected mice. In this embodiment, the speed is set to 30s / turn, and it pauses when moving to the 9th clamping position for blood collection. The collection time for mice is 30s / mouse; the collection time for rats is 60s / mouse. The collection efficiency is equivalent to 33s / mouse for mice, and the working efficiency is about 860 / day, which is 5.3 times higher. The collection efficiency for rats is 63s / mouse, and the working efficiency is about 440 / day, which is 3.6 times higher.
[0038] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. The same components are represented by the same figure marks. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.
Claims
1. An automatic blood sampling device for experimental animals, rats and mice, characterized in that: The invention comprises a base plate (1), a support column (2) being fixedly connected to the base plate (1), a turntable (10) being rotatably connected to the support column (2), a plurality of fixing components (3) being mounted on the turntable (10) for fixing an experimental mouse on the end surface of the turntable (10), the plurality of fixing components (3) being arranged around the axis of the turntable (10) and being placed at the edge of the turntable (10), an automatic blood collection needle (4) being arranged on one side of the base plate (1) for collecting blood from the experimental mouse on the turntable (10), a control component (5) being arranged on one side of the base plate (1) for controlling the automatic blood collection needle (4) to collect blood, a plurality of carbon dioxide masks (6) being arranged on the turntable (10), a gas supply component (7) being arranged on the base plate (1) for conveying carbon dioxide into the carbon dioxide masks (6), and a driving component (8) being arranged on the support column (2) for driving the turntable (10) to rotate.
2. The automatic blood sampling device for experimental animals and mice according to claim 1, characterized in that: The fixing assembly (3) comprises an underarm fixing clip (31), a head and neck fixing clip (32) being provided on one side of the underarm fixing clip (31) close to the center of the rotating disk (10), and both the underarm fixing clip (31) and the head and neck fixing clip (32) being mounted on the rotating disk (10).
3. The automatic blood sampling device for experimental animals and mice according to claim 1, characterized in that: The control component (5) comprises a mechanical arm (51) mounted on the base plate (1), an infrared sensing device (52) being mounted on the upper end of the mechanical arm (51), the infrared sensing device (52) being electrically connected to both the mechanical arm (51) and the automatic collection needle (4), and the automatic collection needle (4) being fixedly connected to the upper end of the mechanical arm (51).
4. The automatic blood sampling device for experimental animals and mice according to claim 1, characterized in that: The gas supply assembly (7) comprises a carbon dioxide gas storage tank, and a plurality of gas supply pipes (72) are fixedly connected to and communicated with the upper portion of the carbon dioxide gas storage tank (71), and the gas supply pipes (72) correspond one-to-one to the carbon dioxide masks (6) and are communicated with the carbon dioxide masks (6).
5. The automatic blood sampling device for experimental animals and mice according to claim 4, characterized in that: A cavity (21) is provided in the support column (2), and the carbon dioxide gas storage tank is fixedly connected in the cavity (21).
6. The automatic blood sampling device for experimental animals and mice according to claim 5, characterized in that: A rotating head (9) is mounted on the upper end of the support column (2); the rotating holes on the rotating head (9) correspond one-to-one with the air supply pipe (72) and are fixedly connected to the air supply pipe (72).
7. The automatic blood sampling device for experimental animals and mice according to claim 6, characterized in that: A boss (101) is provided at the upper end surface of the rotating disk (10); the boss (101) is arranged in an annular shape and is located close to a side of the inner arc surface of the rotating disk (10); and the carbon dioxide mask (6) is placed on a side of the boss (101) away from the center of the rotating disk (10).
8. The automatic blood sampling device for experimental animals and mice according to claim 1, characterized in that: A soft silicone (11) for inserting into the head of an experimental mouse is fixedly connected to the inner wall of the carbon dioxide mask (6); the soft silicone (11) is arranged in a ring shape and surrounds the inner wall of the carbon dioxide mask (6).
9. The automatic blood sampling device for experimental animals and mice according to claim 1, characterized in that: The driving assembly (8) comprises a passive bevel gear ring (82) fixedly connected to the lower end of the rotating disk (10), the passive bevel gear ring (82) being coaxially arranged with the rotating disk (10), an active bevel gear (83) being meshed below the passive bevel gear ring (82), a driving motor (81) being fixedly connected to the supporting column (2), and an output shaft of the driving motor (81) being fixedly connected to the active bevel gear (83).