Multi-layer platelet-rich plasma separation extractor and separation extraction method

Abstract

The invention discloses a multi-layer platelet-rich plasma separation extractor and a separation extraction method, and relates to the technical field of medical instruments. In order to solve the problems that each layer is not easy to completely separate and red blood cells are easy to pour out in an inclined pouring-out process when each layer is extracted after layering of the existing separator, and the recovery rate and the efficiency are relatively low, the following technical scheme is provided: the separation device comprises a drawing rod, a first separation layer, a second separation layer, a third separation layer and a bottom plug, the drawing rod is movably connected in the first separating layer, the first separating layer is movably connected with the second separating layer through the connecting sleeve, and the first separating layer is communicated with the second separating layer; the second separation layer is movably connected with the third separation layer through the supporting sleeve, the second separation layer is communicated with the third separation layer, and the bottom plug is detachably arranged at the bottom of the third separation layer in a sleeving mode. The platelet-rich layer, the plasma layer and the red blood cell layer are efficiently, stably and completely separated in the same extractor, the operation is convenient, and the recovery rate and efficiency of platelets are improved.

Description

Technical Field

[0001] The invention relates to the technical field of medical devices, and in particular to a multilayer platelet-rich plasma separation and extraction device and a separation and extraction method. Background Art

[0002] With the rapid development of biomedical technology, the research on human blood has become more and more complete. Blood is mainly composed of plasma, platelets, white blood cells, and red blood cells. Different components have their corresponding important roles and different medical effects, especially Platelet Rich Plasma (PRP), which can quickly stop bleeding, relieve pain, accelerate wound healing or accelerate local growth, and can greatly reduce the chance of postoperative scar formation. Therefore, PRP is widely used in various surgical operations, heart surgeries, and medical plastic surgeries. However, if red blood cells are mixed in platelets, it will cause inflammation in the affected area; therefore, if these components contained in the blood can be successfully separated to achieve the effect of auxiliary treatment in medicine, it will be able to greatly improve the medical results.

[0003] At present, the common method for separating various components in blood is mainly centrifugation. During centrifugation, blood will be separated from top to bottom into a plasma layer, a buffy coat layer rich in platelets and white blood cells, and a red blood cell layer under high-speed rotation. The prior art often uses a test tube with a flip cover as a container. After separation, the layers are mostly extracted by tilting and pouring. However, during the pouring process, the layers are not easy to be completely separated and the red blood cells are easily poured out, resulting in red blood cells mixed in the buffy coat layer. Alternatively, a syringe is inserted into each layer for extraction. At present, these two methods are inconvenient to operate, and the recovery rate and efficiency of the upper plasma and platelets are low, and there is also a pollution problem. Summary of the invention

[0004] The purpose of the present invention is to provide a multi-layer platelet-rich plasma separation and extraction device and a separation and extraction method to solve the problem that when extracting each layer after stratification using the existing separator, the layers are not easily separated completely and red blood cells are easily poured out during the tilted pouring process, resulting in low recovery rate and efficiency.

[0005] The technical solution of the present invention to solve the above technical problems is as follows:

[0006] A multi-layer platelet-rich plasma separation and extraction device, comprising: a pumping rod, a first separation layer, a second separation layer, a third separation layer and a bottom plug, wherein the pumping rod is movably connected in the first separation layer, the first separation layer is movably connected to the second separation layer through a connecting sleeve, and the first separation layer is communicated with the second separation layer; the second separation layer is movably connected to the third separation layer through a supporting sleeve, and the second separation layer is communicated with the third separation layer, and the bottom plug is detachably sleeved on the bottom of the third separation layer;

[0007] The first separation layer extracts and stores the plasma layer from the third separation layer through a pumping rod; the second separation layer extracts and stores the platelet buffy coat layer from the third separation layer; the third separation layer extracts whole blood that has not been centrifuged and stores the plasma layer, platelet buffy coat layer and red blood cell layer that are sequentially layered from top to bottom after centrifugation.

[0008] Preferably, the first separation layer, the second separation layer and the third separation layer all include a support plate arranged at the upper end and a connecting nozzle arranged at the bottom, a suction tube is penetrated through the connecting nozzles of the first separation layer and the second separation layer, and an injection port for extracting whole blood that has not been centrifuged is provided in the connecting nozzle of the third separation layer, and the connecting nozzle, the suction tube and the injection port are all hollow tubular.

[0009] Preferably, the first separation layer comprises a first separation cylinder, the inner bottom of the first separation cylinder is provided with a first separation plug, a top plug is clamped inside the first separation plug, and the top plug is detachably connected to the pumping rod.

[0010] Preferably, the second separation layer comprises a second separation cylinder, the inner bottom of the second separation cylinder is provided with a second separation plug, and the second separation plug is detachably connected to the connecting sleeve.

[0011] Preferably, hollow external threaded columns are respectively provided in the bottom of the supporting sleeve and the connecting sleeve, the supporting sleeve is threadedly connected to the second separation cylinder via the external threaded column, and the connecting sleeve is threadedly connected to the first separation cylinder via the external threaded column.

[0012] Preferably, the third separation layer comprises a third separation cylinder, and a third separation plug is provided at the inner bottom of the third separation cylinder, and the third separation plug is detachably connected to the lower end surface of the support sleeve.

[0013] A separation and extraction method using a multi-layer platelet-rich plasma separation and extraction device comprises the following steps:

[0014] S1. Unscrew the bottom plug at the lower end of the third separation cylinder, and insert the syringe needle containing whole blood into the injection port at the connecting nozzle at the lower end of the third separation cylinder;

[0015] S2, pull up the support plate of the second separation cylinder, drive the support sleeve threadedly connected to the second separation cylinder to rise, form a storage chamber in the third separation cylinder and push the syringe to inject all the whole blood into the storage chamber;

[0016] S3. The bottom plug is tightened to the connection mouth of the third separation cylinder to form a closed space, and the separation extractor is placed in a centrifuge to perform whole blood centrifugal stratification;

[0017] S4. After centrifugation, remove the separation extractor and extract each layer after centrifugation separately.

[0018] Preferably, step S4 is extracted by one centrifugation, comprising the following steps:

[0019] S41, pulling the pumping rod upward, driving the top plug and the first separation plug connected to the pumping rod to rise to form a cavity, and the passage formed by the extraction tube connected to the second separation cylinder and the first separation cylinder extracts the uppermost plasma in the third separation cylinder into the cavity of the first separation cylinder;

[0020] S42, pulling up the support plate of the first separation cylinder, driving the connecting sleeve and the second separation plug connected to the first separation cylinder to rise to form a cavity, and extracting the platelet layer in the third separation cylinder into the cavity of the second separation cylinder through the extraction tube of the second separation cylinder;

[0021] S43, rotate the support plate of the second separation cylinder, separate the second separation cylinder and the supporting sleeve, take out the first separation cylinder and the second separation cylinder, use the bottom plug to seal the connecting mouth of the second separation cylinder, rotate the support plate of the first separation cylinder, separate the first separation cylinder and the connecting sleeve, take out the first separation cylinder and the pumping rod, and use the bottom plug to seal the connecting mouth of the first separation cylinder.

[0022] Preferably, step S4 is performed by centrifugation twice to extract, comprising the following steps:

[0023] S41, lifting the support plate of the first separation cylinder, driving the connecting sleeve and the second separation plug connected to the first separation cylinder to rise to form a cavity, extracting the plasma layer and the platelet layer in the third separation cylinder into the cavity of the second separation cylinder through the extraction tube of the second separation cylinder, and sealing and separating the red blood cell layer in the third separation cylinder;

[0024] S42, rotating the support plate of the second separation cylinder, separating the second separation cylinder and the supporting sleeve, taking out the first separation cylinder and the second separation cylinder, and sealing the connecting mouth of the second separation cylinder with a bottom plug;

[0025] S43, placing the first separation cylinder and the second separation cylinder into a centrifuge for secondary centrifugation and stratification; after the secondary centrifugation, the first separation cylinder and the second separation cylinder are removed, and the blood is stratified into an upper layer of plasma and a lower layer of platelets in the second separation cylinder;

[0026] S44, pulling the pumping rod, driving the top plug connected to the pumping rod and the first separation plug to rise to form a cavity, and extracting the upper plasma in the second separation cylinder into the cavity of the first separation cylinder through the extraction tube, and the second separation cylinder contains the platelet layer;

[0027] S45, rotating the support plate of the first separation cylinder, separating the first separation cylinder and the connecting sleeve, taking out the first separation cylinder and the pumping rod, and sealing the connecting mouth of the first separation cylinder with a bottom plug.

[0028] Step S44 can also be performed in the following manner: rotating the draw rod to remove the draw rod from the first separation cylinder, using a syringe to puncture the top plug and the first separation plug, and extracting the second layer of platelets separated in the second separation cylinder.

[0029] The present invention has the following beneficial effects:

[0030] Efficient separation and extraction: Through the multi-layer separation design, the plasma layer, platelet buffy coat layer and red blood cell layer can be efficiently separated and extracted. This layered extraction method not only improves the purity of the extraction, but also reduces cross contamination between layers.

[0031] Easy to operate: The separation and extraction device in this technical solution has a reasonable structural design, and the connection and disassembly of various components are achieved through simple mechanical operations, such as lifting the support plate, rotating the support plate, etc., making the entire separation and extraction process easy to operate and easy to master.

[0032] Strong flexibility: This technical solution provides two centrifugal extraction methods (one centrifugation and two centrifugation), and users can choose the appropriate extraction method according to actual needs. One centrifugation is suitable for rapid extraction, while two centrifugation can further improve the accuracy and purity of the extraction.

[0033] Good sealing: Removable sealing connection is adopted between each separation layer and between the separation layer and the bottom plug, which ensures the sealing during the centrifugation process and prevents leakage and contamination of blood during the centrifugation process.

[0034] Reusable: All parts of the separation extractor are detachable, easy to clean and disinfect, so they can be reused, reducing the cost of use.

[0035] Wide scope of application: This technical solution is not only suitable for the separation and extraction of platelet-rich plasma, but can also be used to separate and extract other blood components as needed, and has a wide scope of application.

[0036] Improve medical efficiency: Through the automated separation and extraction process, the complexity and time cost of manual operations are reduced, and the efficiency of medical work is improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 It is a schematic diagram of the overall structure of the multi-layer platelet-rich plasma separation and extraction device of the present invention;

[0038] Figure 2 for Figure 1 Overall structural section diagram;

[0039] Figure 3 This is the overall structural diagram of the first separation layer of the present invention;

[0040] Figure 4 This is a schematic diagram of the overall structure of the second separation layer of the present invention;

[0041] Figure 5 for Figure 4 Schematic diagram of the overall structure of the middle connecting sleeve;

[0042] Figure 6 This is a schematic diagram of the overall structure of the third separation layer of the present invention;

[0043] Figure 7 for Figure 6 mid-section view;

[0044] Figure 8 for Figure 6 Schematic diagram of the overall structure of the middle support sleeve;

[0045] Figures 1 to 8 The reference numerals shown in the figure respectively represent: pumping rod 1, first separation layer 2, first separation cylinder 21, first separation plug 22, top plug 23, second separation layer 3, second separation cylinder 31, second separation plug 32, connecting sleeve 33, third separation layer 4, third separation cylinder 41, third separation plug 42, supporting sleeve 43, bottom plug 5, connecting nozzle 101, suction tube 102, injection port 103, support plate 104, partition plate 105, supporting side plate 106, and external threaded column 107. DETAILED DESCRIPTION

[0046] The technical solution of the present invention is described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0047] Example 1

[0048] Please refer to Figure 1-2 The present invention provides a multi-layer platelet-rich plasma separation and extraction device, which is generally divided into a pumping rod 1, a first separation layer 2, a second separation layer 3, a third separation layer 4 and a bottom plug 5. The first separation layer 2 and the second separation layer 3 are movably connected and interconnected through a connecting sleeve 33, and the second separation layer 3 and the third separation layer 4 are movably connected and interconnected through a supporting sleeve 43. The pumping rod 1 is arranged in the first separation layer 2 and can move therein, and the bottom plug 5 is detachably sleeved on the bottom of the third separation layer 4;

[0049] The third separation layer 4 is used to extract whole blood that has not been centrifuged through the supporting sleeve 43 and to store the plasma layer, platelet buffy coat layer and red blood cell layer that are layered from top to bottom after centrifugation. The first separation layer 2 is used to extract and store the plasma layer from the third separation layer 4 through the pumping rod 1. The second separation layer 3 is used to extract and store the platelet buffy coat layer from the third separation layer 4 through the connecting sleeve 33, thereby achieving complete separation and extraction of each layer in one instrument at the same time, which is easy to operate, improves the separation and extraction efficiency of platelets and plasma, prevents blood from being invaded by bacteria and avoids environmental pollution.

[0050] like Figure 2-Figure 8As shown, the first separation layer 2 includes a first separation cylinder 21, a first separation plug 22 and a top plug 23, the second separation layer 3 includes a second separation cylinder 31, a second separation plug 32 and a connecting sleeve 33, and the third separation layer 4 includes a third separation cylinder 41, a third separation plug 42 and a supporting sleeve 43;

[0051] Among them, a support plate 104 for easy handholding is provided at the upper end of each separation cylinder, a connecting nozzle 101 and a suction tube 102 are provided at the lower ends of the first separation cylinder 21 and the second separation cylinder 31, and a connecting nozzle 101 and an injection port 103 for extracting whole blood that has not been centrifuged are provided at the lower end of the third separation cylinder 41. The connecting nozzle 101, the suction tube 102 and the injection port 103 are all hollow tubular, and the diameter of the connecting nozzle 101 is relatively smaller than that of the separation cylinder, and the diameters of the suction tube 102 and the injection port 103 are smaller than those of the connecting nozzle 101 and are penetrated in the connecting nozzle 101. Each connecting nozzle 101, suction tube 102 or injection port 103 is provided at the vertebral top of the conical partition 105 on the lower end surface of the separation cylinder, and an internal thread is provided on the inner wall of the connecting nozzle 101;

[0052] The walls of each separation cylinder, the conical partition plate 105 and the separation plug arranged inside the separation cylinder and axially movable therein form a chamber for storing each layer of blood; wherein the third separation plug 42 and the conical lower end surface of the support sleeve 43, the second separation plug 32 and the conical lower end surface of the connecting sleeve 33 are movably connected through internal and external threads, and a hollow external threaded column 107 is arranged in the conical bottom of the support sleeve 43 and the connecting sleeve 33, which is respectively threadedly connected with the second separation cylinder 31 and the connecting nozzle 101 of the first separation cylinder 21, and the top plug 23 is clamped in the first separation plug 22 and movably connected with the pumping rod 1 through threads, so that each separation layer can be disassembled and separated, and at the same time, the suction tube 102 of each separation cylinder is aligned with the axis of the injection port 103 and connected, so as to facilitate the sequential extraction of each layer of plasma and platelets after centrifugal separation in the third separation cylinder 41;

[0053] A plurality of reinforced support side plates 106 are arranged on the outer walls of the support sleeve 43 and the connecting sleeve 33. The support side plates 106 can abut against the walls of the third separation cylinder 41 and the second separation cylinder 31, thereby strengthening and stabilizing the sleeves to prevent collisions due to centrifugal force during the centrifugation process, and at the same time facilitate improving stability when extracting blood layers.

[0054] Example 2

[0055] Based on the multi-layer platelet-rich plasma separation and extraction device provided in Example 1, this embodiment provides a method for separating and extracting platelet-rich plasma, and the specific steps are as follows:

[0056] 1. Unscrew the bottom plug 5 at the lower end of the third separation cylinder 41, and insert the syringe needle containing whole blood into the injection port 103 at the connecting nozzle 101 at the lower end of the third separation cylinder 41;

[0057] 2. Pull up the support plate 104 of the second separation cylinder 31, drive the support sleeve 43 threadedly connected to the second separation cylinder 31 to rise, so that a storage chamber is formed in the third separation cylinder 41 and push the syringe to inject all the whole blood into the storage chamber;

[0058] 3. Tighten the bottom plug 5 to the connection nozzle 101 of the third separation cylinder 41 to form a closed space, and then put the entire separation extractor into a centrifuge to perform whole blood centrifugal stratification;

[0059] 4. After centrifugation, remove the entire extractor. There are two ways to extract the layers after centrifugation:

[0060] (1) This method only requires one centrifugation:

[0061] a. Pull the pumping rod 1 upwards, driving the top plug 23 and the first separation plug 22 connected to the pumping rod 1 to rise to form a cavity, and extract the uppermost plasma in the third separation cylinder 41 into the cavity of the first separation cylinder 21 through the passage formed by the extraction tube connected to the second separation cylinder 31 and the first separation cylinder 21;

[0062] b. Pull up the support plate 104 of the first separation cylinder 21, drive the connecting sleeve 33 and the second separation plug 32 threadedly connected with the first separation cylinder 21 to rise to form a cavity, and extract the platelet layer in the third separation cylinder 41 into the cavity of the second separation cylinder 31 through the extraction tube of the second separation cylinder 31;

[0063] c. Rotate the support plate 104 of the second separation cylinder 31 to separate the second separation cylinder 31 from the supporting sleeve 43, and take out the first separation cylinder 21 and the second separation cylinder 31 as a whole, and use the same bottom plug 5 to tighten the connection nozzle 101 of the second separation cylinder 31 for sealing, and similarly rotate the support plate 104 of the first separation cylinder 21 to separate the first separation cylinder 21 from the connecting sleeve 33 and take out the first separation cylinder 21 and the pumping rod 1, and use the same bottom plug 5 to tighten the connection nozzle 101 of the first separation cylinder 21 for sealing, thereby realizing the separation and extraction of platelets and plasma at the same time, and finally push or extract them when needed;

[0064] (2) This method requires two centrifugations to completely separate the red blood cells, plasma, and platelet layers; the first centrifugation mainly separates the red blood cells, and the second centrifugation mainly separates the plasma and platelets:

[0065] a. Pull up the support plate 104 of the first separation cylinder 21, drive the connecting sleeve 33 and the second separation plug 32 threadedly connected to the first separation cylinder 21 to rise to form a cavity, and extract the plasma layer and the platelet layer in the third separation cylinder 41 into the cavity of the second separation cylinder 31 through the extraction tube of the second separation cylinder 31, and the red blood cell layer remains in the third separation cylinder 41 for sealed separation;

[0066] b. Rotate the support plate 104 of the second separation cylinder 31 to separate the second separation cylinder 31 from the support sleeve 43, and take out the first separation cylinder 21 and the second separation cylinder 31 as a whole, and use the same bottom plug 5 to tighten the connection nozzle 101 of the second separation cylinder 31 to seal it;

[0067] c. Put the first separation cylinder 21 and the second separation cylinder 31 into a centrifuge as a whole for secondary centrifugation and stratification; after the secondary centrifugation, remove the first separation cylinder 21 and the second separation cylinder 31 as a whole, and the blood is stratified into an upper layer of plasma and a lower layer of platelets in the second separation cylinder 31;

[0068] d. Pull the pumping rod 1 upwards, driving the top plug 23 and the first separation plug 22 connected to the pumping rod 1 to rise to form a cavity, and extract the upper plasma in the second separation cylinder 31 into the cavity of the first separation cylinder 21 through the extraction tube, and the remaining in the second separation cylinder 31 is the platelet layer;

[0069] e. Rotate the support plate 104 of the first separation cylinder 21 to separate the first separation cylinder 21 and the connecting sleeve 33, and take out the first separation cylinder 21 and the pumping rod 1. At the same time, use the same bottom plug 5 to tighten the connecting mouth 101 of the first separation cylinder 21 to seal it, so as to separate and extract platelets and plasma at the same time, and push or pump them out when needed.

[0070] It should be noted that step d can also be implemented in the following manner: in order to reduce the contamination of platelet extraction, the pumping rod 1 can be rotated separately to be taken out of the first separation cylinder 21 separately, and a syringe with a needle is taken out to puncture the top plug 23 and the first separation plug 22 and then directly extract the second layer of platelets separated in the second separation cylinder 31. Of course, the first layer of plasma can be extracted first, and then the second layer of platelets can be extracted.

[0071] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A multilayer platelet-rich plasma separation and extraction device, characterized in that: include: A pumping rod (1), a first separation layer (2), a second separation layer (3), a third separation layer (4) and a bottom plug (5), wherein the pumping rod (1) is movably connected in the first separation layer (2), the first separation layer (2) is movably connected to the second separation layer (3) via a connecting sleeve (33), and the first separation layer (2) and the second separation layer (3) are in communication; the second separation layer (3) is movably connected to the third separation layer (4) via a supporting sleeve (43), and the second separation layer (3) and the third separation layer (4) are in communication, and the bottom plug (5) is detachably sleeved on the bottom of the third separation layer (4); The first separation layer (2) extracts and stores the plasma layer from the third separation layer (4) through the extraction rod (1); the second separation layer (3) extracts and stores the platelet buffy coat layer from the third separation layer (4); the third separation layer (4) extracts whole blood that has not been centrifuged and stores the plasma layer, platelet buffy coat layer and red blood cell layer that are sequentially layered from top to bottom after centrifugation.

2. The multilayer platelet-rich plasma separation and extraction device according to claim 1, characterized in that: The first separation layer (2), the second separation layer (3) and the third separation layer (4) all comprise a support plate (104) arranged at the upper end and a connecting nozzle (101) arranged at the bottom; a suction tube (102) is provided through the connecting nozzles (101) of the first separation layer (2) and the second separation layer (3); an injection port (103) for extracting whole blood that has not been centrifuged is provided in the connecting nozzle (101) of the third separation layer (4); the connecting nozzle (101), the suction tube (102) and the injection port (103) are all hollow tubular.

3. The multilayer platelet-rich plasma separation and extraction device according to claim 1, characterized in that: The first separation layer (2) comprises a first separation cylinder (21), the inner bottom of the first separation cylinder (21) is provided with a first separation plug (22), a top plug (23) is clamped inside the first separation plug (22), and the top plug (23) is detachably connected to the pumping rod (1).

4. The multilayer platelet-rich plasma separation and extraction device according to claim 3, characterized in that: The second separation layer (3) comprises a second separation cylinder (31), the inner bottom of the second separation cylinder (31) is provided with a second separation plug (32), and the second separation plug (32) is detachably connected to the connecting sleeve (33).

5. The multilayer platelet-rich plasma separation and extraction device according to claim 4, characterized in that: A hollow external thread column (107) is respectively arranged at the bottom of the supporting sleeve (43) and the connecting sleeve (33); the supporting sleeve (43) is threadedly connected to the second separation cylinder (31) via the external thread column (107); and the connecting sleeve (33) is threadedly connected to the first separation cylinder (21) via the external thread column (107).

6. The multi-layer platelet-rich plasma separation and extraction device according to claim 1, characterized in that: The third separation layer (4) comprises a third separation cylinder (41), the inner bottom of the third separation cylinder (41) is provided with a third separation plug (42), and the third separation plug (42) is detachably connected to the lower end surface of the support sleeve (43).

7. A separation and extraction method using the multilayer platelet-rich plasma separation and extraction device according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1, unscrewing the bottom plug (5) at the lower end of the third separation cylinder (41), and inserting the syringe needle containing whole blood into the injection port (103) at the connecting nozzle (101) at the lower end of the third separation cylinder (41); S2, lifting the support plate (104) of the second separation cylinder (31), driving the support sleeve (43) threadedly connected to the second separation cylinder (31) to rise, forming a storage chamber in the third separation cylinder (41) and pushing the syringe to inject all the whole blood into the storage chamber; S3, the bottom plug (5) is tightened to the connection mouth (101) of the third separation cylinder (41) to form a closed space, and the separation extractor is placed in a centrifuge to perform whole blood centrifugal stratification; S4. After centrifugation, remove the separation extractor and extract each layer after centrifugation separately.

8. The multi-layer platelet-rich plasma separation and extraction method according to claim 7, characterized in that: Step S4 is to extract by centrifugation once, comprising the following steps: S41, pulling the pumping rod (1) upwards, driving the top plug (23) and the first separation plug (22) connected to the pumping rod (1) to rise to form a cavity, and the passage formed by the extraction tube connected to the second separation cylinder (31) and the first separation cylinder (21) extracts the uppermost layer of plasma in the third separation cylinder (41) into the cavity of the first separation cylinder (21); S42, pulling up the support plate (104) of the first separation cylinder (21), driving the connecting sleeve (33) and the second separation plug (32) connected to the first separation cylinder (21) to rise to form a cavity, and extracting the platelet layer in the third separation cylinder (41) into the cavity of the second separation cylinder (31) through the extraction tube of the second separation cylinder (31); S43, rotate the support plate (104) of the second separation cylinder (31), separate the second separation cylinder (31) and the supporting sleeve (43), take out the first separation cylinder (21) and the second separation cylinder (31), use the bottom plug (5) to seal the connecting mouth (101) of the second separation cylinder (31), rotate the support plate (104) of the first separation cylinder (21), separate the first separation cylinder (21) and the connecting sleeve (33), take out the first separation cylinder (21) and the pumping rod (1), and use the bottom plug (5) to seal the connecting mouth (101) of the first separation cylinder (21).

9. The multi-layer platelet-rich plasma separation and extraction method according to claim 7, characterized in that: Step S4 is extracted by two centrifugations, comprising the following steps: S41, lifting the support plate (104) of the first separation cylinder (21), driving the connecting sleeve (33) and the second separation plug (32) connected to the first separation cylinder (21) to rise to form a cavity, extracting the plasma layer and the platelet layer in the third separation cylinder (41) into the cavity of the second separation cylinder (31) through the extraction tube of the second separation cylinder (31), and sealing and separating the red blood cell layer in the third separation cylinder (41); S42, rotating the support plate (104) of the second separation cylinder (31), separating the second separation cylinder (31) and the supporting sleeve (43), taking out the first separation cylinder (21) and the second separation cylinder (31), and sealing the connecting nozzle (101) of the second separation cylinder (31) with the bottom plug (5); S43, placing the first separation cylinder (21) and the second separation cylinder (31) into a centrifuge for secondary centrifugation and stratification; after the secondary centrifugation, removing the first separation cylinder (21) and the second separation cylinder (31), and stratifying the blood in the second separation cylinder (31) into an upper layer of plasma and a lower layer of platelets; S44, pulling the pumping rod (1), driving the top plug (23) and the first separation plug (22) connected to the pumping rod (1) to rise to form a cavity, and extracting the upper plasma in the second separation cylinder (31) into the cavity of the first separation cylinder (21) through the extraction tube, and the second separation cylinder (31) contains the platelet layer; S45, rotate the support plate (104) of the first separation cylinder (21), separate the first separation cylinder (21) and the connecting sleeve (33), take out the first separation cylinder (21) and the pumping rod (1), and use the bottom plug (5) to seal the connecting mouth (101) of the first separation cylinder (21).

10. The multi-layer platelet-rich plasma separation and extraction method according to claim 9, characterized in that: Step S44 can also be performed in the following manner: rotating the draw rod (1) to remove the draw rod (1) from the first separation cylinder (21), using a syringe to puncture the top plug (23) and the first separation plug (22), and extracting the second layer of platelets separated in the second separation cylinder (31).

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