Automatic sterile tray inoculation equipment

Through automated sterile shallow disc inoculation equipment, automatic vaccination is achieved using conveyor belts and pump injection systems, and combined with disinfection devices and sponge block design in the rotating cylinder, the pollution and consistency problems during manual vaccination are solved, and the production efficiency and Product stability.

CN119220390AInactive Publication Date: 2024-12-31GUANGZHOU YUANDONG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202411620986.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-14
Publication Date
2024-12-31
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

In the prior art, the process of artificial inoculation of bacterial cellulose has problems such as pollution risk, high labor intensity, low production efficiency and poor product consistency, which is difficult to meet the needs of industrialized large-scale production.

Method used

An automated sterile shallow disc inoculation equipment was designed, and automated inoculation was carried out using conveyor belt, culture medium peristaltic pump and seed liquid injection pump. It was combined with the electric heating plate and ultraviolet lamp in the rotating cylinder to disinfect the inoculation needle, and passed through the sponge block. and the setting of magnetic blocks reduces the risk of pollution.

Benefits of technology

Automatic and accurate vaccination operations in a sterile environment are achieved, which reduces the risk of pollution, improves production efficiency and product consistency, ensures uniform disinfection of vaccination needles, and reduces the uncertainty of manual operation.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses automatic sterile tray inoculation equipment which comprises a shell, fixing plates are fixedly connected to the inner walls of the front side and the rear side of the shell, the left sides and the right sides of the two fixing plates extend to the outside, feeding ports are formed in the left side and the right side of the shell, and two rotating rods are jointly and rotationally connected to the adjacent sides of the two fixing plates; the two rotating rods are fixedly connected with conveying rollers, every two matched conveying rollers are in transmission connection through a conveying belt, the two conveying belts penetrate through the two feeding ports, and the inner bottom of the shell is fixedly connected with two lifting motors. According to the equipment, automatic and accurate inoculation operation can be achieved in a sterile environment, the pollution risk is reduced, the labor intensity is reduced, the production efficiency and the product consistency are improved, and through the arrangement of the rotary disinfection assembly and the sponge block, after inoculation is completed every time, the inoculation needle can be rapidly and uniformly sterilized and disinfected, and the production efficiency is improved. Therefore, the pollution risk is reduced.
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Description

Technical Field

[0001] The invention relates to the technical field of bioengineering, and in particular to an automated aseptic shallow dish inoculation device. Background Art

[0002] Bacterial cellulose is a material with broad application prospects. In its production process, the inoculation step is crucial. At present, the traditional inoculation steps in this field are mostly completed manually. Specifically, operators need to use tools such as inoculation needles and culture media to perform manual operations under strict sterile conditions.

[0003] However, this artificial inoculation method has many disadvantages. First, it has extremely high requirements for the sterile environment. Any slight omission may lead to contamination, which seriously affects the production quality of bacterial cellulose. It is difficult and costly to maintain such a highly sterile environment in actual operation. Second, the entire operation process is extremely cumbersome, involving many steps and delicate operations, which not only consumes a lot of manpower and time, but also has high labor intensity, which greatly limits the improvement of production efficiency. Third, artificial inoculation is easily disturbed by environmental factors (such as temperature, humidity, air quality, etc.) and human factors (such as the skill level, proficiency, concentration, etc. of the operator), and it is difficult to ensure the stability and consistency of the inoculation operation. Fourth, in terms of the control of the inoculation amount, it is difficult to achieve precise control by manual operation, which makes the bacterial cellulose products produced have a large risk of differences in quality and performance, and it is difficult to ensure the consistency of the products, and it is impossible to meet the market's requirements for product quality stability.

[0004] More importantly, with the growing demand for bacterial cellulose applications in various fields, large-scale industrial production has become an inevitable trend. However, due to its own limitations, the existing artificial inoculation method cannot achieve automation and continuity of the inoculation process, and is far from meeting the urgent needs of industrial large-scale production for efficient, stable and accurate inoculation.

[0005] Therefore, it is necessary to design a new automated sterile shallow dish inoculation equipment to solve the above problems. Summary of the invention

[0006] The purpose of the present invention is to solve the shortcomings existing in the prior art and to propose an automated sterile shallow tray inoculation device, which can realize automatic and precise inoculation operations in a sterile environment, reduce the risk of contamination, reduce labor intensity, and improve production efficiency and product consistency. By setting up a rotating disinfection component and a sponge block, the inoculation needle can be quickly and evenly sterilized and disinfected after each inoculation, thereby reducing the risk of contamination.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] An automated aseptic shallow tray inoculation device comprises a shell, wherein the inner walls on both the front and rear sides of the shell are fixedly connected with fixed plates, the left and right sides of the two fixed plates extend to the outside, the left and right sides of the shell are provided with feed ports, the adjacent sides of the two fixed plates are rotatably connected with two rotating rods, the two rotating rods are fixedly connected with conveying rollers, every two matched conveying rollers are connected through conveyor belt transmission, the two conveyor belts pass through the two feed ports, the inner bottom of the shell is fixedly connected with two lifting motors, the telescopic ends of the two lifting motors are fixedly connected with a bracket, and a seed liquid storage tank and a seed liquid injection port are installed on the left inner wall of the shell. injection pump, the inner bottom of the seed liquid storage tank is connected with the liquid inlet end of the seed liquid injection pump through a short tube, the right inner wall of the shell is equipped with a culture medium storage tank and a culture medium peristaltic pump, the liquid inlet end of the culture medium peristaltic pump is connected with the inner bottom of the culture medium storage tank, the liquid outlet end of the culture medium peristaltic pump is connected with a culture medium addition tube, the left and right inner walls of the shell are connected with threaded rods for rotation together, the threaded rods are threadedly connected with a transverse block, the lower end of the transverse block is fixedly connected with an inoculation needle, the inoculation needle is connected with the liquid outlet end of the seed liquid injection pump through a hose, two sterilization lamps are installed on the inner top of the shell, and a positive pressure air supply filter assembly is installed on the right side of the shell.

[0009] Preferably, a rotating cylinder is provided in the shell, a notch is provided on the right side of the rotating cylinder, a plurality of electric heating plates and ultraviolet lamps are provided on the inner wall of the rotating cylinder, and the inoculation needle is located in the rotating cylinder.

[0010] Preferably, it also includes a rotating sterilization component, which includes a fixed rod fixed on the left inner wall of the shell, a fixed ring is fixedly connected to the right side of the fixed rod, a rotating groove is provided in the fixed ring, a rotating ring is provided in the rotating groove, the inner wall of the rotating ring is fixedly connected to the rotating cylinder, a plurality of tooth edges are fixedly connected to the outer wall of the rotating cylinder, a fixed block is fixedly connected to the right side of the seed liquid storage tank, the lower end of the fixed block is rotatably connected to a rotating rod, a second gear is provided on the rotating rod, and the second gear is meshed with the plurality of tooth edges.

[0011] Preferably, a delayed rotation component is provided on the left side of the shell, and the delayed rotation component includes a rectangular box arranged on the left side of the shell, the left side of the threaded rod extends into the rectangular box, the inner bottom and inner top of the rectangular box are jointly rotatably connected with a vertical rod, a threaded layer is provided on the vertical rod, and a first bevel gear is provided on the vertical rod and the threaded rod, a lifting block is threadedly connected on the vertical rod, the lifting block is slidably connected to the inner wall of the rectangular box, a moving plate is slidably connected in the rectangular box, the lower end of the moving plate is elastically connected to the inner bottom of the rectangular box by a first spring, the vertical rod passes through the moving plate, the right side of the transverse block is fixedly connected to a pressure rod, and the left inner wall of the shell is fixedly connected to a pressure sensor.

[0012] Preferably, a rack is fixedly connected to the lower end of the movable plate, a transmission rod is rotatably connected to the left inner wall of the outer shell, a first gear is provided on the transmission rod, the first gear and the rack are meshed with each other, and second bevel gears meshing with each other are provided on the transmission rod and the rotating rod.

[0013] Preferably, the front and rear inner walls of the shell are fixedly connected with strip boxes, the inner walls on opposite sides of the two strip boxes are embedded with second electromagnets, the two strip boxes are slidably connected with sliders, the two sliders are elastically connected to the adjacent sides of the corresponding second electromagnets through a second spring, and the adjacent sides of the two sliders are fixedly connected with sponge blocks.

[0014] Preferably, a transverse plate is fixedly connected to the left inner wall of the shell, a trapezoidal block, a magnetic block and two conductive bars are fixedly connected to the lower end of the transverse plate, a damping box is fixedly connected to the upper end of the transverse block, and an iron block is slidably connected inside the damping box.

[0015] The present invention has the following beneficial effects:

[0016] 1. Compared with the prior art, the conveyor belt, the culture medium peristaltic pump and the seed liquid injection pump are arranged, after the culture plate enters the shell, it can automatically locate and inject the seed liquid and culture medium into the culture plate, thereby realizing the automatic inoculation process, greatly improving the production efficiency and product consistency;

[0017] 2. Compared with the prior art, the electric heating plate and ultraviolet lamp in the rotating cylinder can be arranged to disinfect the inoculation needle when the inoculation needle is reset after each injection, thereby reducing the risk of contamination;

[0018] 3. Compared with the prior art, by setting the delayed rotation component and the rotary sterilization component, after each inoculation needle is reset, the downward movement of the movable plate drives the rotating cylinder to rotate, so that the ultraviolet light is evenly irradiated on various positions of the inoculation needle, and at the same time ensures that various positions of the inoculation needle are evenly heated, further improving the sterilization and disinfection effect of the inoculation needle;

[0019] 4. Compared with the prior art, by setting the second electromagnet and the sponge block, each time the inoculation needle moves back, the two sponge blocks will absorb the seed liquid remaining on the inoculation needle, so that bacteria will not be generated on the inoculation needle due to the residual seed liquid in the future;

[0020] 5. Compared with the prior art, through the arrangement of the trapezoidal blocks and the magnetic blocks, each time the lateral movement block moves to the right, the distance between the two sponge blocks is farther, and each time the lateral movement block moves to the left, the adjacent surfaces of the two sponge blocks contact each other, so that when the inoculation needle moves to the left, the two sponge blocks will absorb the excess seed liquid, and when the inoculation needle moves to the right, the sterilized inoculation needle is prevented from touching the sponge block to cause bacteria to adhere.

[0021] In summary, the present invention has many significant advantages over the prior art. First, with the help of a conveyor belt, a culture medium peristaltic pump and a seed liquid injection pump, the culture plate can be automatically positioned after entering the outer shell to complete the injection of the seed liquid and the culture medium, thereby realizing automated inoculation and greatly improving production efficiency and product consistency. Secondly, the arrangement of the electric heating plate and the ultraviolet lamp in the rotating cylinder can disinfect the inoculation needle when it is reset, reducing the risk of contamination. Furthermore, the delayed rotation component cooperates with the rotating sterilization component to evenly heat and illuminate each position of the inoculation needle, further enhancing the sterilization and disinfection effect. In addition, the second electromagnet and the sponge block can absorb the residual seed liquid in the inoculation needle to prevent bacterial growth. Finally, the design of the trapezoidal block and the magnetic block allows the sponge block to open and close as needed, avoiding contamination of the disinfection needle when moving to the right, and absorbing excess seed liquid when moving to the left, thereby optimizing the inoculation process in all directions. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the structure of an automated aseptic shallow tray inoculation device proposed by the present invention;

[0023] Figure 2 for Figure 1 A schematic diagram of the structure from another perspective;

[0024] Figure 3 for Figure 1 A partial perspective view of

[0025] Figure 4 for Figure 1 Frontal internal sectional view of;

[0026] Figure 5 for Figure 1 A side internal cross-sectional view of

[0027] Figure 6 It is a schematic diagram of the structure of the rotary sterilization component;

[0028] Figure 7 for Figure 6 Schematic diagram of the structure of the middle fixed ring;

[0029] Figure 8 Schematic diagram of the structure of 6 rotating cylinders.

[0030] In the figure: 1 housing, 2 fixed plate, 3 rotating rod, 4 conveyor belt, 5 rectangular box, 6 transmission rod, 7 feed port, 8 first gear, 9 rack, 10 drive motor, 11 positive pressure air supply filter assembly, 12 threaded rod, 13 first bevel gear, 14 vertical rod, 15 lifting block, 16 moving plate, 17 first spring, 18 sterilization lamp, 19 culture medium storage tank, 20 pressure sensor, 21 culture medium peristaltic pump, 22 culture medium addition tube, 23 seed liquid storage tank, 24 transverse block, 25 resistance 1. a nitrile box, 26 a seed liquid injection pump, 27 an inoculating needle, 28 a sponge block, 29 a fixed block, 30 a rotating rod, 31 a fixed ring, 32 a fixed rod, 33 a second gear, 34 a second bevel gear, 35 a rotating cylinder, 36 a heating plate, 37 an ultraviolet lamp, 38 a notch, 39 a tooth edge, 40 a rotating ring, 41 a hose, 42 an iron block, 43 a horizontal plate, 44 a trapezoidal block, 45 a conductive strip, 46 a magnetic block, 47 a strip box, 48 a slider, 49 a second electromagnet, 50 a lifting motor, and 51 a bracket. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.

[0032] Reference Figure 1-Figure 8, an automated aseptic shallow tray inoculation device, comprising a shell 1, the inner walls of the front and rear sides of the shell 1 are fixedly connected with fixed plates 2, the left and right sides of the two fixed plates 2 are extended to the outside, the left and right sides of the shell 1 are provided with feed ports 7, the adjacent sides of the two fixed plates 2 are connected to two rotating rods 3 for common rotation, the two rotating rods 3 are fixedly connected with conveying rollers, and every two matched conveying rollers are connected by transmission through conveyor belts 4. When inoculating, the culture tray is placed on the two conveyor belts 4, and the conveying motor of the prior art is installed on the fixed plate 2, so that the conveying motor The end of the output shaft is fixedly connected to the rotating rod 3 on the left side, so that the operation of the conveying motor drives the two conveyor belts 4 to run, and then the culture trays placed on the two conveyor belts 4 are transported to the shell 1. The two conveyor belts 4 pass through two feed ports 7. The inner bottom of the shell 1 is fixedly connected to two lifting motors 50. The telescopic ends of the two lifting motors 50 are commonly fixedly connected to a bracket 51. The bracket 51 can be replaced accordingly according to the type of culture tray selected. A seed liquid storage tank 23 and a seed liquid injection pump 26 are installed on the left inner wall of the shell 1. The seed liquid The inner bottom of the storage tank 23 is connected to the liquid inlet end of the seed liquid injection pump 26 through a short tube. The culture medium storage tank 19 and the culture medium peristaltic pump 21 are installed on the right inner wall of the shell 1. A sliding door is provided on the rear side of the shell 1 to facilitate the subsequent replacement of the bracket 51 and the addition of raw materials to the seed liquid storage tank 23 and the culture medium storage tank 19. The liquid inlet end of the culture medium peristaltic pump 21 is connected to the inner bottom of the culture medium storage tank 19, and the liquid outlet end of the culture medium peristaltic pump 21 is connected to the culture medium addition tube 22. The left and right inner walls of the shell 1 are connected to threaded rods 12 that rotate together. A transverse block 24 is threadedly connected to the threaded rod 12, and an inoculation needle 27 is fixedly connected to the lower end of the transverse block 24. The inoculation needle 27 is connected to the liquid outlet of the seed liquid injection pump 26 through a hose 41. Two sterilization lamps 18 are installed on the inner top of the outer shell 1, and a positive pressure air supply filter assembly 11 is installed on the right side of the outer shell 1. The positive pressure air supply filter assembly 11 is equipped with an H14 grade filter element, so that the operating area becomes a vertical flow clean area to prevent external pollution from entering. Two baffles are preset on the outside. After each culture plate enters the outer shell 1, the two feed ports 7 are blocked and sealed by the baffles.

[0033] Among them, a rotating cylinder 35 is provided in the outer shell 1, a notch 38 is provided on the right side of the rotating cylinder 35, a plurality of electric heating plates 36 and an ultraviolet lamp 37 are provided on the inner wall of the rotating cylinder 35, the inoculation needle 27 is located in the rotating cylinder 35, the power of the ultraviolet lamp 37 is 10W, the temperature of the electric heating plate 36 is 200°C, and the running time for each disinfection is more than 2S. It also includes a rotating sterilization component, which includes a fixed rod 32 fixed on the inner wall of the left side of the outer shell 1, a fixed ring 31 is fixedly connected to the right side of the fixed rod 32, a rotating groove is provided in the fixed ring 31, a rotating ring 40 is provided in the rotating groove, the inner wall of the rotating ring 40 is fixedly connected to the rotating cylinder 35, and a plurality of tooth edges 39 are fixedly connected to the outer wall of the rotating cylinder 35, a fixed block 29 is fixedly connected to the right side of the seed liquid storage tank 23, and the lower end of the fixed block 29 is rotatably connected to the rotating rod 30, and a second gear 33 is provided on the rotating rod 30, and the second gear 33 is meshed with the plurality of tooth edges 39.

[0034] Among them, a delayed rotation component is provided on the left side of the shell 1, and the delayed rotation component includes a rectangular box 5 arranged on the left side of the shell 1, and the left side of the threaded rod 12 extends into the rectangular box 5. The inner bottom and inner top of the rectangular box 5 are rotatably connected with a vertical rod 14 together, and a threaded layer is provided on the vertical rod 14. The vertical rod 14 and the threaded rod 12 are both provided with a first bevel gear 13. A lifting block 15 is threadedly connected to the vertical rod 14, and a first electromagnet is embedded in the lifting plate 15. The lifting block 15 is slidably connected to the inner wall of the rectangular box 5, and a moving plate 16 is slidably connected in the rectangular box 5. The moving plate 16 is made of iron material and is provided with a controller. When the transverse block 24 moves to the right to the specified position, the staff controls the first electromagnet to be energized through the controller. After the first electromagnet is energized, it generates an attractive force on the moving plate 16, and the lower end of the moving plate 16 is elastically connected to the inner bottom of the rectangular box 5 through the first spring 17 The vertical rod 14 passes through the movable plate 16, and a pressure rod is fixedly connected to the right side of the transverse block 24. A pressure sensor 20 is fixedly connected to the left inner wall of the shell 1. When the transverse block 24 moves back to its original position, the pressure sensor 20 is squeezed to generate an electrical signal transmitted to the controller. The controller controls the first electromagnet to cut off the power. A rack 9 is fixedly connected to the lower end of the movable plate 16, and a transmission rod 6 is rotatably connected to the left inner wall of the shell 1. A first gear 8 is provided on the transmission rod 6, and a one-way bearing is provided between the transmission rod 6 and the first gear 8. The one-way bearing has the characteristics of free rotation in one direction and locking in one direction, so that when the rack 9 moves up, the first gear 8 cannot rotate the transmission rod 6. When the rack 9 moves down, the transmission rod 6 is driven to rotate by the first gear 8. The first gear 8 and the rack 9 are meshed with each other. The transmission rod 6 and the rotating rod 30 are both provided with a second bevel gear 34 meshing with each other.

[0035] Among them, the inner walls of the front and rear sides of the shell 1 are fixedly connected with strip boxes 47, the inner walls of the opposite sides of the two strip boxes 47 are embedded with second electromagnets 49, and sliders 48 are slidably connected in the two strip boxes 47. A portion of the slider 48 close to the corresponding second electromagnet 49 is made of magnetic material, and the two sliders 48 are elastically connected to the adjacent sides of the corresponding second electromagnet 49 through a second spring. The adjacent sides of the two sliders 48 are fixedly connected with sponge blocks 28, and the left inner wall of the shell 1 is fixedly connected with a transverse plate 43, and the lower end of the transverse plate 43 is fixedly connected with a trapezoidal block 44, a magnetic block 46 and two conductive strips 45, and the upper end of the transverse block 24 is fixedly connected with a damping box 25. An iron block 42 is slidably connected in the damping box 25. When the iron block 42 moves to the right, it will not contact the two conductive strips 45. After the transverse block 24 moves to the right to the specified position, the magnetic block 46 attracts the iron block 42 to move upward. When the transverse block 24 moves to the left, the magnetic block 46 separates from the iron block 42. At this time, the iron block 42 will not move downward due to the large friction with the inner wall of the damping box 25, so that the iron block 42 will contact the two conductive strips 45. The two second electromagnets 49 are energized to generate a repulsive force on the two sliders 48. A power supply is provided on the outer shell 1. The iron block 42 and the two conductive strips 45 form a conductive unit. The power supply, the conductive unit and the two second electromagnets 49 form a loop through wires.

[0036] The functional principle of the present invention can be explained through the following operation mode: the culture tray is placed on two conveyor belts 4, and the operation of the conveying motor of the prior art drives the two conveyor belts 4 to operate, so as to move the culture tray into the housing 1. When the culture tray is moved to a suitable position, the two lifting motors 50 are controlled to operate, so that the bracket 51 moves up to lift the culture tray to a suitable height. The staff controls the output shaft of the driving motor 10 to rotate clockwise, drives the inoculation needle 27 to move right to a specified position through the lateral movement block 24, controls the seed liquid injection pump 26 and the culture medium peristaltic pump 21 to operate, and quantitatively adds the culture medium and seed liquid into the culture tray;

[0037] When the lateral block 24 moves to the right, the vertical rod 14 is driven to rotate by the first bevel gear 13, and the vertical rod 14 rotates to move the lifting block 15 downward, so that the lifting block 15 contacts the upper end of the moving plate 16. When injecting the culture medium and the seed liquid, the staff controls the operation of the first electromagnet through the controller, so that the first electromagnet generates an attractive force on the moving plate 16. When the injection is completed, the output shaft of the driving motor 10 is controlled to rotate counterclockwise, so that the lateral block 24 moves to the left.

[0038] When the threaded rod 12 rotates counterclockwise, the two first bevel gears 13 drive the vertical rod 14 to rotate in the opposite direction, so that the lifting block 15 drives the movable plate 16 to move up, and the movable plate 16 moves up to make the rack 9 move up. Since a one-way bearing is provided between the first gear 8 and the transmission rod 6, the one-way bearing has the performance of rotating in one direction and locking in the other direction, so that the rotation of the first gear 8 will not drive the transmission rod 6 to rotate. When the transverse block 24 moves back to its original position, the pressure rod will squeeze the pressure sensor 20, and the pressure sensor 20 will generate an electrical signal transmitted to the controller. The controller controls the first electromagnet to cut off the power. After the first electromagnet is powered off, it no longer has an attractive force on the movable plate 16. Under the action of the first spring 17, the movable plate 16 drives the rack 9 to move down. At this time, the transmission rod 6 is driven to rotate through the first gear 8, and then the rotating rod 30 is driven to rotate under the action of the two second bevel gears 34. Since the second gear 33 is in a meshing state with multiple tooth edges 39, the rotating cylinder 35 is driven to rotate.

[0039] Each time the inoculation needle 27 moves to the left after injecting the seed liquid, it will enter the rotating cylinder 35 through the notch 38, and the pressure sensor 20 will be squeezed to generate an electrical signal transmitted to the controller, and the controller will control the multiple electric heating plates 36 and the ultraviolet lamp 37 to operate for a period of time to disinfect the inoculation needle 27. Since the rotating cylinder 35 is in a rotating state, it is ensured that each position of the inoculation needle 27 can be evenly disinfected.

[0040] When the traverse block 24 moves right, the iron block 42 is far away from the cross plate 43, and the iron block 42 will not contact the two conductive strips 45, so that when the traverse block 24 moves right, the two second electromagnets 49 will not be energized. When the traverse block 24 moves to the specified position, the magnetic block 46 will attract the iron block 42, so that the iron block 42 moves up. When the traverse block 24 moves left, the iron block 42 will separate from the magnetic block 46. Since the inner wall of the damping box 25 is relatively rough, the iron block 42 will not move down and reset. When the traverse block 24 moves left, the iron block 42 will contact the two conductive strips 45, and the two second electromagnets 49 will be energized.

[0041] After the two second electromagnets 49 are energized, they exert a repulsive force on the corresponding sliders 48, so that the two sliders 48 drive the corresponding sponge blocks 28 to move relative to each other and squeeze together. When the lateral block 24 drives the inoculation needle 27 to move left, the inoculation needle 27 will move between the two sponge blocks 28, so that the sponge blocks 28 absorb the seed liquid adhered to the inoculation needle 27. When the inoculation needle 27 is separated from the two sponge blocks 28, the iron block 42 is no longer in contact with the two conductive strips 45. Under the action of the two second springs, the sliders 48 and the sponge blocks 28 are reset. As the lateral block 24 continues to move, the iron block 42 will contact the trapezoidal block 44, so that the iron block 42 enters the damping box 25 by mutual squeezing.

[0042] When the inoculation is completed, the lifting motor 50 is controlled to retract, so that the bracket 51 moves downward, and the inoculated culture tray is placed on the two conveyor belts 4, and the culture tray is transported to the right side of the housing 1 by the operation of the conveyor belts 4.

[0043] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. An automated aseptic shallow tray inoculation device, comprising a housing (1), characterized in that: The front and rear inner walls of the shell (1) are fixedly connected to fixed plates (2), and the left and right sides of the two fixed plates (2) extend to the outside. The left and right sides of the shell (1) are provided with feed ports (7). The adjacent sides of the two fixed plates (2) are rotatably connected to two rotating rods (3), and the two rotating rods (3) are fixedly connected to conveying rollers. Every two matched conveying rollers are connected by transmission through conveyor belts (4), and the two conveyor belts (4) pass through the two feed ports (7). The inner bottom of the shell (1) is fixedly connected to two lifting motors (50), and the telescopic ends of the two lifting motors (50) are fixedly connected to a bracket (51). A seed liquid storage tank (23) and a seed liquid injection pump (26) are installed on the left inner wall of the shell (1), and the inner bottom of the seed liquid storage tank (23) is connected to the seed liquid injection pump (26). The liquid inlet of the injection pump (26) is connected through a short tube. A culture medium storage tank (19) and a culture medium peristaltic pump (21) are installed on the right inner wall of the shell (1). The liquid inlet of the culture medium peristaltic pump (21) is connected to the inner bottom of the culture medium storage tank (19). The liquid outlet of the culture medium peristaltic pump (21) is connected to a culture medium addition tube (22). The left and right inner walls of the shell (1) are connected to threaded rods (12) for rotation. A transverse block (24) is threadedly connected to the threaded rods (12). The lower end of the transverse block (24) is fixedly connected to an inoculation needle (27). The inoculation needle (27) is connected to the liquid outlet of the seed liquid injection pump (26) through a hose (41). Two sterilization lamps (18) are installed on the inner top of the shell (1). A positive pressure air supply filter assembly (11) is installed on the right side of the shell (1).

2. The automated aseptic shallow tray inoculation device according to claim 1, characterized in that: A rotating cylinder (35) is arranged inside the housing (1), a notch (38) is arranged on the right side of the rotating cylinder (35), a plurality of electric heating plates (36) and ultraviolet lamps (37) are arranged on the inner wall of the rotating cylinder (35), and the inoculation needle (27) is located inside the rotating cylinder (35).

3. The automated aseptic shallow tray inoculation device according to claim 2, characterized in that: The invention also comprises a rotary sterilization component, which comprises a fixed rod (32) fixed on the left inner wall of the housing (1); a fixed ring (31) is fixedly connected to the right side of the fixed rod (32); a rotating groove is provided in the fixed ring (31); a rotating ring (40) is provided in the rotating groove; the inner wall of the rotating ring (40) is fixedly connected to a rotating cylinder (35); a plurality of tooth edges (39) are fixedly connected to the outer wall of the rotating cylinder (35); a fixed block (29) is fixedly connected to the right side of the seed liquid storage tank (23); a rotating rod (30) is rotatably connected to the lower end of the fixed block (29); a second gear (33) is provided on the rotating rod (30); the second gear (33) is meshed with the plurality of tooth edges (39).

4. The automated aseptic shallow tray inoculation device according to claim 3, characterized in that: A time-delay rotation assembly is provided on the left side of the shell (1), and the time-delay rotation assembly comprises a rectangular box (5) arranged on the left side of the shell (1), the left side of the threaded rod (12) extends into the rectangular box (5), the inner bottom and the inner top of the rectangular box (5) are rotatably connected to a vertical rod (14), a threaded layer is provided on the vertical rod (14), and the vertical rod (14) and the threaded rod (12) are both provided with a first bevel gear (13), a lifting block (15) is threadedly connected to the vertical rod (14), and the lifting block (15) is slidably connected to the inner wall of the rectangular box (5), a moving plate (16) is slidably connected inside the rectangular box (5), the lower end of the moving plate (16) is elastically connected to the inner bottom of the rectangular box (5) by a first spring (17), the vertical rod (14) penetrates the moving plate (16), the right side of the transverse block (24) is fixedly connected to a pressure rod, and the left inner wall of the shell (1) is fixedly connected to a pressure sensor (20).

5. The automated aseptic shallow tray inoculation device according to claim 4, characterized in that: The lower end of the movable plate (16) is fixedly connected to a rack (9); a transmission rod (6) is rotatably connected to the left inner wall of the housing (1); a first gear (8) is provided on the transmission rod (6); the first gear (8) and the rack (9) are meshed with each other; and second bevel gears (34) are meshed with each other on the transmission rod (6) and the rotating rod (30).

6. The automated aseptic shallow tray inoculation device according to claim 1, characterized in that: The inner walls on both the front and rear sides of the housing (1) are fixedly connected with strip boxes (47), the inner walls on the opposite sides of the two strip boxes (47) are embedded with second electromagnets (49), the two strip boxes (47) are slidably connected with sliders (48), the two sliders (48) are elastically connected to the adjacent sides of the corresponding second electromagnets (49) through second springs, and the adjacent sides of the two sliders (48) are fixedly connected with sponge blocks (28).

7. The automated aseptic shallow tray inoculation device according to claim 1, characterized in that: A transverse plate (43) is fixedly connected to the left inner wall of the housing (1); a trapezoidal block (44), a magnetic block (46) and two conductive bars (45) are fixedly connected to the lower end of the transverse plate (43); a damping box (25) is fixedly connected to the upper end of the transverse block (24); and an iron block (42) is slidably connected inside the damping box (25).