Cell incubator and humidity control method

Abstract

In order to solve the problems of unstable humidity and poor sterilization effect in the prior art, the invention provides a cell incubator and a humidity control method, the cell incubator comprises an incubator inner cavity, an outer door and a sealing structure, the incubator inner cavity is provided with a biological decontamination system, the biological decontamination system comprises an active humidification system and an airflow residue removal system, and the active humidification system is connected with the airflow residue removal system. The active humidifying system comprises a sterilizing agent input port, a water input port and an evaporator for evaporating a sterilizing agent and water, and the evaporator is mounted on the inner wall of the inner cavity of the incubator through an evaporator mounting seat. By means of the efficient sterilization function and the stable sealing structure, the sterile state of the inner cavity can be kept, the purity of the experimental environment is ensured, in addition, the humidifying device further has the capacity of accurately controlling the humidifying amount, humidity control is effectively stabilized, and more ideal experimental conditions are provided.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and in particular to a cell culture box and a humidity control method. Background Art

[0002] Cell culture incubators are commonly used experimental equipment in the biomedical field. They are mainly used to simulate the growth environment of cells in the body to study the basic physiological activities of cells, the occurrence and development of diseases, and the mechanism of action of drugs. With the continuous deepening of biomedical research, the technology of cell culture incubators is also constantly developing and improving.

[0003] At present, there are many types of cell culture incubators on the market, including constant temperature and humidity incubators, carbon dioxide incubators, sterile incubators, etc. These incubators have their own advantages and disadvantages, but in general, they all have the following basic functions: Temperature control: The cell culture incubator needs to maintain a constant temperature to simulate the growth environment of cells in the body.

[0004] Humidity control: The cell culture incubator needs to maintain a certain humidity to prevent the cells from drying out.

[0005] Gas control: For some cell types, such as mammalian cells, a certain carbon dioxide concentration needs to be provided to maintain normal cell growth.

[0006] Sterile environment: The cell culture incubator needs to be kept sterile to avoid contamination.

[0007] However, existing cell culture incubators still have some problems, such as unstable humidity and poor sterilization effect, etc. These problems may affect cell growth and the accuracy of experimental results.

[0008] In order to solve the above problems, the Chinese utility model patent with patent number CN206872848U proposes a cell culture box, including a cell culture box, which is composed of multiple parts, including: a box body, a baffle, a partition, a water tank, a humidifier, a water storage bottle, an air inlet, an air filter, a fan, an air outlet, a lighting lamp, a heating plate and an operation panel. However, the utility model does not take into account that cells need a sterile environment and may be contaminated, so there are defects. Summary of the invention

[0009] The present invention mainly solves the problems of unstable humidity and poor sterilization effect in the prior art, and provides a cell culture box and a humidity control method.

[0010] The above technical problems of the present invention are mainly solved by the following technical solutions: A cell culture box comprises an inner cavity and an outer door of the culture box, characterized in that it also comprises a sealing structure, the inner cavity of the culture box is provided with a biological decontamination system, the biological decontamination system comprises an active humidification system and an airflow residue removal system, the active humidification system comprises a sterilant input port, a water input port and an evaporator for evaporating the sterilant and water, and the evaporator is mounted on the inner wall of the inner cavity of the culture box through an evaporator mounting seat.

[0011] As a preferred solution, the active humidification system further includes a pump for providing power and a valve for adjusting the pipeline, and the sterilizing agent input port and the water input port are respectively connected to the valve through filters.

[0012] As a preferred solution, the evaporator includes a bottom support plate and an evaporator inner core, the bottom support plate is connected to the evaporator inner core, and a temperature control component for controlling the temperature is provided inside the evaporator inner core.

[0013] As a preferred solution, the temperature control component includes a temperature sensor and a heating rod.

[0014] As a preferred solution, a liquid injection pipe and a wall joint wrapping the liquid injection pipe are provided at the top of the evaporator.

[0015] As a preferred solution, the outer layer of the evaporator is provided with a thermal insulation jacket for heat insulation.

[0016] As a preferred solution, the external structure is the same as the active humidification system, and the airflow removal system includes a circulation fan for airflow circulation, a guide plate, and a catalyst arranged under the guide plate.

[0017] As a preferred solution, the sealing structure includes an inner door sealing strip for inner cavity sealing, a decorative sealing strip for outer flange surface decoration and an outer door sealing strip for heat insulation sealing.

[0018] As a preferred solution, a liquid sensor for detecting whether there is liquid in the tube is provided behind the pump.

[0019] A humidity control method comprises the following steps: S1. After inputting the target humidity, read the current humidity value and calculate the difference between the current humidity value and the target humidity; S2. Determine the amount of water to be added and perform evaporative humidification at a certain speed and quantity; S3, when the amount of water to be added remains the first set value, the water is evaporated slowly intermittently, with a standing time in between, and the humidity is read in real time through the incubator humidity sensor; S4, when the humidity reaches the target concentration, it enters the maintenance stage. When the humidity drops to the second set value, a small amount of water is added for evaporation supplement.

[0020] Therefore, the advantages of the present invention are: 1. Active humidification, humidity control is more precise and stable; 2. Efficient sterilization function; 3. Stable sealing structure. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural diagram of the present invention.

[0022] Figure 2 It is a partial cross-sectional structural diagram of the present invention.

[0023] Figure 3 It is the active humidification system of the present invention.

[0024] Figure 4 It is the biological decontamination system of the present invention.

[0025] Figure 5 It is a structural diagram of the evaporator of the present invention.

[0026] Figure 6 2 is a structural diagram of the sealing structure of the present invention.

[0027] Figure 7 It is the humidity control logic diagram of the present invention.

[0028] Figure 8 This is the test data of 90% humidity control in the incubator of the present invention.

[0029] Fig. 9 It is a sterilization effect verification diagram of the present invention.

[0030] In the figure: 1. Sterilizer filler; 2. Water tank; 3. Evaporator; 4. Sensor assembly; 5. Circulation fan; 6. Heating assembly; 7. Catalyst; 8. Inner door handle; 11. Liquid outlet joint; 12. Liquid sensor; 13. Pump; 14. Valve; 15. Filter; 16. Sterilizer inlet; 17. Water inlet; 18. Mounting bracket; 19. Incubator cavity; 21. Guide plate; 31. Insulation jacket; 32. Temperature sensor; 33. Heating rod; 34. Evaporator mounting base; 35. Bottom support plate; 36. Evaporator inner core; 37. Liquid injection pipe; 38. Wall joint; 41. Outer door; 42. Inner door glass; 43. Inner door sealing strip; 44. Decorative sealing strip; 45. Outer door sealing strip; 46. Incubator flange surface. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is further specifically described below through embodiments and in conjunction with the accompanying drawings.

[0032] In order to solve the problems of unstable humidity and poor sterilization effect in the prior art, the present invention provides a cell culture box and a humidity control method.

[0033] Embodiment 1: As an advanced biological experimental equipment, this cell culture box has many key functions and features. First of all, it is equipped with an evaporator 3, a circulating fan 5, a catalyst 7, a water tank 2 and a sterilant filler 1. The synergistic effect of these devices enables the incubator to independently perform VHP steam sterilization and cyclic decomposition and residue removal operations. Through efficient sterilization function and stable sealing structure, this incubator can maintain the sterility of the inner cavity and ensure the purity of the experimental environment. In addition, the incubator also has the ability to accurately control the humidification amount and effectively stabilize the humidity control, thereby providing more ideal experimental conditions. The outer door is provided with an inner door to ensure sealing. At the same time, the design of installing an inner door handle 8 on the inner door makes the operation more convenient. It also has an air jacket heating component 6, a constant temperature system, a sensor component 4, etc., which further improves the performance and practicality.

[0034] A cell culture box includes a culture box cavity 19, an outer door 41 with an inner door glass 42 on one side near the culture box cavity 19, and a sealing structure. The culture box cavity 19 is provided with a biological decontamination system that can be efficiently sterilized. The biological decontamination system includes an active humidification system that can make humidity control more accurate and stable and an airflow removal system for removing hydrogen peroxide residues after sterilization. The active humidification system includes an evaporator 3 connected in sequence, a pump 13 providing power, a mounting bracket 18, a valve 14 for adjusting the pipeline, a filter 15 for filtering bacteria, a sterilant input port 16 and a water input port 17, the sterilant input port 16 and the water input port 17 are connected to the valve 14 through the filter 15, respectively, and the top shape of the evaporator 3 is a concave disk. In actual operation, this culture box can not only meet the needs of cell culture, but also can be widely used in multiple fields such as biomedicine and laboratory scientific research. Its stable working performance and advanced functional design make it one of the indispensable important equipment in the laboratory. In general, the multiple functions and characteristics of this cell culture box provide convenience for scientific researchers and promote the smooth progress of experimental work.

[0035] As an important component of the cell culture chamber, the evaporator 3 plays a vital role in the laboratory. Its precise structural design and efficient functional characteristics make it one of the indispensable equipment in the experimental process. The structure of the evaporator 3 in the present invention is as follows: Figure 5As shown, the evaporator 3 is mounted on the inner wall of the incubator cavity 19 through the evaporator mounting seat 34, and a wire outlet is left in the design. Such a design is intended to ensure the stability and ease of use of the evaporator 3. The evaporator core 36 is the core. The interior of the evaporator core 36 is provided with a temperature control component for temperature control. The temperature control component includes a temperature sensor 32 and a heating rod 33. These components together constitute the main temperature control system of the evaporator 3. The design of the temperature control component plays a vital role in the performance of the evaporator 3. The temperature sensor 32 can accurately sense the temperature changes inside the evaporator 3, and the heating rod 33 can be adjusted accordingly according to the information fed back by the sensor, thereby realizing accurate control of the internal temperature of the evaporator 3. The accuracy of this temperature control can not only provide a stable experimental environment, but also ensure the accuracy and repeatability of the experimental results.

[0036] The evaporator 3 includes a bottom support plate 35 and an evaporator core 36. The bottom support plate 35 and the evaporator core 36 are connected by threads to fix the heating rod 33 and the temperature sensor 32. This connection method is not only simple and reliable, but also helps to ensure the close connection between the heating rod 33 and the temperature sensor 32 and the evaporator core 36, effectively improving the accuracy and stability of temperature control. In addition, the design of the evaporator core 36 has also been carefully considered to ensure the uniform distribution and stability of temperature, which plays a vital role in the experimental process.

[0037] In general, as a key component in the cell culture incubator, the evaporator 3 provides reliable support for laboratory work with its exquisite structural design and efficient temperature control system. Through the reasonable layout of the evaporator 3 and the precise adjustment of the internal temperature control components, scientific researchers can control the experimental conditions more accurately, thereby improving the credibility of the experimental results and the quality of scientific research results. Therefore, the optimized design and functional characteristics of the evaporator 3 make it an indispensable and important equipment in biological experiments, providing strong support for the smooth progress of scientific research.

[0038] The top of the evaporator 3 is shaped like a concave dish for receiving sterilizing agent and water, where evaporation is performed. The design of this concave dish makes the evaporation process more efficient and controllable. For the convenience of operation, an injection pipe 37 and a through-wall joint 38 wrapped around the injection pipe 37 are provided above the concave dish of the evaporator 3. Through the injection pipe 37, liquid to be evaporated, such as sterilizing agent or water, can be added to the evaporator 3. In this way, the evaporator 3 can gradually evaporate the liquid as needed to achieve the desired concentration or separation effect. The existence of the through-wall joint 38 is to ensure the isolation of the injection pipe 37 from the inside of the evaporator 3 to prevent external impurities from entering the inside of the evaporator 3. The outer layer of the evaporator 3 is provided with an insulating jacket 31 for heat insulation and heat preservation, which plays a role in heat insulation and heat preservation. It can effectively reduce the heat exchange between the evaporator 3 and the external environment, thereby reducing energy loss. The role of heat insulation and heat preservation enables the evaporator 3 to better maintain a stable operating temperature, improve evaporation efficiency and reduce energy consumption.

[0039] In summary, the design of the evaporator 3 takes into account several key factors to ensure its efficient, controllable and stable operation. Through the design of the top concave plate, the setting of the injection pipe 37 and the wall joint 38, the evaporator 3 can conveniently perform liquid injection and evaporation operations. At the same time, the application of the thermal insulation jacket 31 can effectively isolate the heat exchange between the evaporator 3 and the external environment, providing a good thermal insulation effect. These design and functional features have made important contributions to the reliability and performance optimization of the evaporator 3, making it one of the indispensable equipment in laboratories and industrial production.

[0040] Figure 3 The active humidification system of the incubator is a commonly used device in laboratories and industrial production, which is used to control the humidity in the environment and keep the environmental conditions stable. The active humidification system of the present invention has a unique design, and sterilizing agent and water are respectively input through the sterilizing agent input port 16 and the water input port 17 to ensure the hygienic safety during the humidification process. Such a design can effectively avoid the pollution problem caused by unclean water source or sterilizing agent, and improve the reliability and stability of the humidification system.

[0041] In the active humidification system, the sterilizing agent and water input through the input port will be filtered through the filter 15 to remove the microbial contaminants therein and ensure the hygienic safety of the humidification process. Afterwards, they will meet at the valve 14, and the system will automatically adjust the pipeline according to the program to achieve the operation of adding water or adding sterilizing agent. This intelligent pipeline adjustment design enables the humidification system to accurately control the input and mixing ratio of the liquid according to actual needs, thereby achieving precise adjustment of the ambient humidity.

[0042] Through this intelligent design, the active humidification system can operate more efficiently, improving humidification efficiency and stability. The system can automatically input and mix liquids according to the set parameters and procedures without manual intervention, reducing the possibility of operational errors and improving work efficiency. This intelligent design not only improves the performance of the humidification system, but also provides a more convenient and reliable environmental control solution for laboratories and industrial production.

[0043] In order to ensure the stable operation of the system, the power of the active humidification system comes from the pump 13 installed on the mounting bracket 18. The pump 13 and the evaporator 3 are connected through the liquid outlet joint 11. The function of the pump 13 is to transport water and sterilizing agent from the input port to the valve 14 to achieve liquid mixing and output. In addition, a liquid sensor 12 is set behind the pump 13 to detect whether there is liquid in the pipe. This design can detect the liquid flow in the pipeline in time and provide feedback signals to the control system to ensure the stability and accuracy of liquid delivery.

[0044] By using the detection signal provided by the liquid sensor 12, the active humidification system can accurately know the liquid discharge time. Combined with the pre-set pump flow parameters, the system can accurately calculate the amount of liquid to be added, thereby achieving quantitative filling of the liquid. This intelligent liquid filling method enables the humidification system to automatically and accurately fill the liquid according to demand without manual intervention, greatly improving the system's automation and ease of operation.

[0045] In general, the active humidification system achieves precise regulation and stable control of environmental humidity through intelligent pipeline adjustment and liquid filling control. Its intelligent design and automated operation make the humidification system play an important role in laboratories and industrial production, providing reliable support for maintaining a suitable humidity environment. This intelligent humidification system not only improves work efficiency, but also effectively reduces the complexity of manual operation, bringing convenience and reliability to laboratories and industrial production.

[0046] like Figure 7 The figure is a humidity control logic diagram. The on-off and flow rate of the pump 13 are controlled by the following logic through the real-time humidity value fed back by the humidity sensor in the incubator. Figure 7 These are test data for 90% humidity control in an incubator.

[0047] The biological decontamination system of this incubator includes the following three aspects: When adding sterilizing agent and water, sterilize through filter 15; Evaporator 3 performs evaporation and diffusion sterilization on the sterilizing agent (hydrogen peroxide solution); Strict sealing design prevents external pollutants from entering the box.

[0048] like Figure 4 The design of the biological decontamination system takes into account several key aspects to ensure the cleanliness and safety of the culture environment. First, the system sterilizes through the filter 15 when adding sterilizing agent and water. This step can effectively remove suspended microorganisms and impurities to ensure the purity of the input liquid. The sterilization effect of the filter 15 can avoid the introduction of harmful microorganisms at the initial stage, effectively ensuring the cleanliness of the internal environment of the incubator.

[0049] Secondly, evaporator 3 plays an important role in the system, evaporating and diffusing the sterilant (such as hydrogen peroxide solution) for sterilization. Evaporator 3 uses heating to evaporate the sterilant and diffuse it throughout the incubator, thereby achieving a sterilization effect on the air and surface. This evaporation and diffusion sterilization method can fully cover every corner of the incubator, ensuring the comprehensiveness and durability of the biological decontamination effect, and providing a reliable disinfection guarantee for the culture environment.

[0050] In addition, the incubator also adopts a strict sealing design to prevent external contaminants from entering the box. The sealing design can effectively isolate the entry of external air, microorganisms and particulate matter, and avoid the influence of cross-contamination and unclean factors. Such a sealing design ensures the stability and purity of the internal environment of the incubator, and provides a safe and reliable working space for biological experiments. In summary, the biological decontamination system of the present invention has efficient and comprehensive functions in sterilization, evaporative diffusion sterilization and strict sealing design. Through the combination of multiple protective measures, the system can ensure the cleanliness and safety of the culture environment, and provide reliable support and guarantee for scientific research experiments and biological culture.

[0051] The biological decontamination system of the present invention adopts VHP steam sterilization as a biological decontamination method. In addition to the active humidification system, the airflow residue removal system includes a circulation fan 5 for airflow circulation, a guide plate 21, and a catalyst 7 arranged under the guide plate 21 for accelerating the decomposition of residual hydrogen peroxide. This design is intended to ensure the cleanliness and safety of the internal environment of the incubator through efficient VHP (hydrogen peroxide vapor) sterilization technology.

[0052] The quantitative hydrogen peroxide solution passes through the filter 15, valve 14, pump 13 and liquid sensor 12 in sequence, and is accumulated in the evaporation tray 3 for evaporation. In this process, the system can accurately add and evaporate the hydrogen peroxide solution by accurately controlling the operation of the liquid sensor 12 and pump 13, thereby ensuring the stability and controllability of steam generation. This quantitative liquid addition and evaporation method can effectively improve the sterilization effect and disinfection uniformity, and provide reliable biological decontamination protection for the cell culture chamber.

[0053] Inside the incubator, the circulating fan 5 is designed to circulate the airflow, and the liquid addition is stopped after the sterilization is completed. Such a design can ensure that the steam is fully distributed and diffused throughout the incubator, thereby achieving a comprehensive sterilization effect on all surfaces and microorganisms in the air. At the same time, the circulating fan 5 continues to guide the airflow through the catalyst 7 under the guide plate 21 for accelerating the decomposition of residual hydrogen peroxide to remove the residual hydrogen peroxide. This step can further enhance the sterilization effect, ensure that the microorganisms inside the incubator are completely removed, and provide a clean and safe working environment for biological research experiments.

[0054] In general, the present invention adopts VHP steam sterilization technology, and realizes comprehensive biological decontamination of the internal environment of the incubator through the design of the circulating fan 5, the guide plate 21 and the catalyst 7 for accelerating the decomposition of residual hydrogen peroxide in the airflow removal system. The efficient and accurate sterilization method and the internal circulation structure ensure the clean environment in the incubator, providing reliable support and guarantee for scientific research experiments and biological cultivation. Such a biological decontamination system can not only meet the needs of laboratories and biological industries, but also has high safety and reliability, which is of great significance for ensuring the accuracy of experimental data and the success of biological cultivation.

[0055] like Fig. 9 To verify the sterilization effect of the biological decontamination system in this incubator. When conducting the sterilization effect verification experiment of the biological decontamination system, the staff adopted a rigorous experimental design and operation process. First of all, in this experiment, the staff quantitatively added 1 gram of 35% hydrogen peroxide to the biological decontamination system in the incubator to ensure that the expected sterilization effect can be achieved in the subsequent experimental process. Hydrogen peroxide, as a commonly used disinfectant, has strong oxidizing properties and can effectively kill bacteria, viruses and other microorganisms, thereby achieving the purpose of sterilization.

[0056] After adding hydrogen peroxide, the staff set two time points for the rehydration operation, 10 minutes and 20 minutes respectively. At these two time points, the staff added 1 gram of hydrogen peroxide to ensure that the hydrogen peroxide concentration in the incubator can continue to reach more than 400ppm and can be maintained for more than 30 minutes. This operation design is intended to ensure that the disinfection effect during the experiment can meet the expected requirements and ensure the reliability and accuracy of the experimental results.

[0057] By verifying the sterilization effect of the biological decontamination system in the incubator, the staff can evaluate the actual effect of hydrogen peroxide in the disinfection process, and adjust and optimize the disinfection plan accordingly to improve the disinfection effect and efficiency. The experimental results will provide important reference information for the staff, guide subsequent experimental work and application practice, further improve the design and application of the biological decontamination system, and promote its application and promotion in environmental governance and cleaning.

[0058] The sealing structure is the last key component of the present invention, including the inner door sealing strip 43, the decorative sealing strip 44 and the outer door sealing strip 45, each of which performs different functions. First, the inner door sealing strip 43 is located at the inner cavity sealing position, installed at the bottom of the inner door glass 42, and fits with the flange surface 46 of the incubator. Its main function is to ensure the sealing of the internal space of the equipment and prevent gas or liquid leakage, thereby keeping the internal environment clean and stable. Secondly, the decorative sealing strip 44 is mainly used for the outer flange surface, which not only plays a sealing role, but also can beautify the appearance of the equipment, enhance the overall decorative effect, and make the appearance of the equipment more elegant and professional. Finally, the outer door sealing strip 45 is mainly used for heat insulation sealing, which effectively prevents heat conduction and loss, improves the heat insulation performance of the equipment, and ensures the stability of the internal temperature when the equipment is running in a high temperature environment. These different types of sealing strips together constitute a perfect sealing structure, which provides important guarantees for the normal operation and performance of the equipment.

[0059] Embodiment 2: A humidity control method, applicable to the cell culture box in the first embodiment, comprises the following steps: S1. After inputting the target humidity, read the current humidity value and calculate the difference between the current humidity value and the target humidity.

[0060] S2. Determine the amount of water to be added and perform quantitative evaporation humidification at a certain faster speed.

[0061] S3, when the amount of water to be added remains the first set value, evaporate slowly intermittently, leave a static time in between, and read the humidity in real time through the incubator humidity sensor. The first set value of the present application can be 1g.

[0062] S4, when the humidity reaches the target concentration, enter the maintenance stage, when the humidity drops to the second set value, add a small amount of water for evaporation supplement. The second set value of the present application can be 0.5%.

[0063] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. A cell culture incubator, comprising an inner chamber and an outer door, characterized in that: It also includes a sealing structure. The inner cavity of the incubator is provided with a biological decontamination system, which includes an active humidification system and an airflow residue removal system. The active humidification system includes a sterilant input port, a water input port and an evaporator for evaporating the sterilant and water. The evaporator is installed on the inner wall of the inner cavity of the incubator through an evaporator mounting seat.

2. A cell culture incubator according to claim 1, characterized in that: The active humidification system also includes a pump for providing power and a valve for adjusting the pipeline, and the sterilant input port and the water input port are respectively connected to the valve through filters.

3. A cell culture incubator according to claim 1, characterized in that: The evaporator comprises a bottom support plate and an evaporator inner core, wherein the bottom support plate is connected to the evaporator inner core, and a temperature control component for controlling the temperature is arranged inside the evaporator inner core.

4. A cell culture incubator according to claim 3, characterized in that: The temperature control component includes a temperature sensor and a heating rod.

5. A cell culture incubator according to claim 1, characterized in that: A concave dish for receiving sterilizing agent and water is arranged on the top of the evaporator, and a liquid injection pipe and a wall-penetrating joint wrapping the liquid injection pipe are arranged above the concave dish.

6. A cell culture incubator according to claim 1, 3 or 5, characterized in that: The outer layer of the evaporator is provided with a heat-insulating jacket for heat insulation.

7. A cell culture incubator according to claim 1, characterized in that: The airflow residue removal system comprises a circulation fan for airflow circulation, a guide plate, and a catalyst arranged below the guide plate for accelerating the decomposition of residual hydrogen peroxide.

8. The cell culture incubator according to claim 1, characterized in that: The sealing structure comprises an inner door sealing strip for inner cavity sealing, a decorative sealing strip for outer flange surface decoration and an outer door sealing strip for heat insulation sealing.

9. A cell culture incubator according to claim 2, characterized in that: A liquid sensor for detecting whether there is liquid in the tube is arranged behind the pump.

10. A humidity control method, applicable to the cell culture incubator according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. After inputting the target humidity, read the current humidity value and calculate the difference between the current humidity value and the target humidity; S2. Determine the amount of water to be added and perform evaporative humidification at a certain speed and quantity; S3, when the amount of water to be added remains the first set value, the water is evaporated slowly intermittently, and a static time is left in between, and the humidity is read in real time through the incubator humidity sensor; S4, when the humidity reaches the target concentration, it enters the maintenance stage. When the humidity drops to the second set value, a small amount of water is added for evaporation supplement.

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