Online microfluidic monitoring device and use method thereof

Abstract

The invention discloses an on-line microfluidic monitoring device, comprising: a water intake pump for pumping a water sample to be detected in a water source and transferring the water sample to a water sample pool; the water sample tank is used for temporarily storing a water sample; the reagent placing area is used for storing a detection reagent; the liquid transferring assembly comprises a liquid transferring gun and a moving device, and the liquid transferring assembly is used for transferring the water sample and the reagent. According to the present invention, the water sample can be quantitatively extracted from the target water source at the fixed time, the water sample of the part is subjected to filtration treatment, the filtered water sample is transferred into the corresponding digestion bottles in batches, the corresponding reagent is added into the corresponding digestion bottle, heating digestion is performed, the reagent of the part is extracted and transferred into the disc chip for detection, and the detection data is fed back in real time; the process does not need manual intervention and can be remotely and automatically completed, manpower resources are effectively saved, and detection result errors caused by personal errors are avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of water quality monitoring equipment, and in particular to an online microfluidic monitoring device and a use method thereof. Background Art

[0002] As an emerging high-efficiency, trace, convenient, accurate and advanced technology, microfluidic water quality testing technology is very accurate. The microfluidic chip can change the flow direction of water samples in a space at the micron level. Due to its small size, low reagent consumption required for detection, and the ability to make fluids and reagents flow in one direction and quantitatively mix, it can be used to control the injection sequence and injection volume of different reagents or water sample fluids. It also has the advantage of being replaceable. For example, it can be organically combined with portable water quality testing equipment, which is particularly suitable for on-site real-time sampling and testing of water samples.

[0003] However, traditional testing in the prior art requires people to perform the testing process on site, and real-time monitoring is required for water quality sensitive areas, which requires testers to go to the site for testing frequently. The manual testing and sampling operations are cumbersome, and human errors are inevitable during the operation, affecting the accuracy of the test results. In order to solve the above problems, an online microfluidic monitoring device and a method of using the same are proposed to solve the above problems. Summary of the invention

[0004] The purpose of the present invention is to provide an online microfluidic monitoring device and a method of using the same in order to overcome the above-mentioned shortcomings.

[0005] In order to solve the above technical problems, the present invention adopts the following technical solution: an online microfluidic monitoring device, comprising:

[0006] A water pump, which is used to extract the water sample to be tested from the water source and transfer it to the water sample pool;

[0007] Water sample pool, used for temporary storage of water samples;

[0008] Reagent storage area, used to store test reagents;

[0009] A pipetting assembly, comprising a pipetting gun and a moving device, and used for transferring the water sample and the reagent;

[0010] A digestion component, the digestion component includes a digestion bottle for receiving the water sample and the reagent, the digestion component also includes a sealing device and a heating device, the heating device is used to heat and digest the water sample and the reagent in the digestion bottle, and the sealing device is used to seal the digestion bottle to prevent the reagent and water sample in the digestion bottle from evaporating during the digestion process;

[0011] A monitoring component is provided with a disc chip and a puncturing mechanism, and the monitoring component is used to detect the digested reagents and water samples and to feed back the detection data in real time.

[0012] Further, it also includes a vacuum chuck mechanism, a chip storage area and a chip placement area;

[0013] The vacuum suction cup mechanism is used to replace the used disc chip in the monitoring component and transfer it to the chip placement area, and take out a new disc chip from the chip storage area.

[0014] Furthermore, it also includes a pneumatic assembly, which includes an air compressor, a pneumatic three-way valve and a vacuum generator;

[0015] The pneumatic assembly is used to drive the vacuum suction cup mechanism to absorb the sealing device, the puncturing mechanism and the disc chip.

[0016] Furthermore, the vacuum suction cup mechanism includes a movable slide rail and a lifting seat arranged on the movable slide rail;

[0017] The output end of the lifting seat is provided with a chip suction cup and a sealing suction cup for adsorbing the sealing device, the puncturing mechanism and the disc chip;

[0018] The output end of the lifting seat is also provided with an air guide pipe and a negative pressure pipe, the negative pressure pipe is used to connect the chip suction cup and the sealing suction cup, and the negative pressure pipe is provided with an adjustment member in the middle, the adjustment member is used to control the chip suction cup or the sealing suction cup to generate negative pressure;

[0019] The adjusting member includes a driving unit and an air guide member corresponding to the driving unit.

[0020] Furthermore, the chip storage area and the chip placement area are provided with lifting drive motors;

[0021] The lifting drive motor is used to control the height of the stacked disc chips in the chip storage area and the chip placement area, so that the vacuum suction cup mechanism can transfer the used disc chips to the chip placement area and take out the new disc chips at the corresponding position in the chip storage area.

[0022] Further, the number of the digestion bottles is four groups;

[0023] The four groups of digestion bottles are respectively a COD digestion bottle, a TP digestion bottle, a TN digestion bottle and an NH4H digestion bottle.

[0024] Furthermore, the number of the pipetting components is also four, and they correspond one to one to the four groups of digestion bottles;

[0025] A flushing device is also provided in the pipetting assembly, and the flushing device is used to clean the pipette after the pipette absorbs the water sample or the reagent.

[0026] Furthermore, a filtering device is provided in the water sample pool;

[0027] The filtering device is used to filter the water sample extracted by the water pump.

[0028] Further, the pneumatic three-way valve is connected to the output end of the filtering device;

[0029] The pneumatic three-way valve is used to introduce the gas generated by the air compressor into the filter device from the output end of the filter device to backwash the filter device.

[0030] The present invention adopts another technical solution. A method for using the online microfluidic monitoring device comprises the following steps:

[0031] S1. Extract water samples from the water source at regular intervals and in fixed quantities;

[0032] S2. After filtering the water sample, transfer the water sample to the COD digestion bottle, TP digestion bottle, TN digestion bottle and NH4H digestion bottle respectively through a pipette and a mobile device for driving the transfer;

[0033] S3, rinse the pipette, and add the corresponding reagent into the corresponding digestion bottle through the pipette again;

[0034] S4, sealing the digestion bottle in step S3, and then heating and digesting;

[0035] S5. After the digestion is completed, the digested water sample mixture is finally transferred to the disc chip by a pipette, and after the liquid capsule set on the disc chip is punctured, a centrifugal test is performed;

[0036] S6. After the test is completed, the used disk chip is transferred to the chip storage area, and a new disk chip is taken out from the chip storage area and placed at the test point for subsequent testing.

[0037] The beneficial effects of the present invention are embodied in:

[0038] The present invention can extract water samples from a target water source at a regular and quantitative time, and after filtering the water samples, transfer them to corresponding digestion bottles in batches, and then add corresponding reagents to the corresponding digestion bottles and heat and digest them, extract the reagents and transfer them to a disk chip for detection, and feed back the detection data in real time to achieve the effect of remote online monitoring. The above process does not require human intervention and can be completed remotely and automatically, which effectively saves human resources and avoids errors in detection results caused by human errors. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 It is a schematic diagram of the process of the present invention;

[0040] Figure 2 It is a structural schematic diagram of the present invention;

[0041] Figure 3 It is a schematic diagram of the structure of the vacuum suction cup mechanism of the present invention.

[0042] In the figure:

[0043] 1. Disc chip; 2. Chip storage area; 3. Mobile slide rail; 4. Chip placement area; 5. Digestion component; 51. COD digestion bottle; 52. TP digestion bottle; 53. TN digestion bottle; 54. NH4H digestion bottle; 6. Piercing mechanism; 7. Sealing device; 8. Vacuum suction cup mechanism; 81. Chip suction cup; 82. Sealing suction cup; 83. Adjustment part; 84. Lifting seat; 85. Air guide tube; 86. Negative pressure tube; 87. Air guide part. DETAILED DESCRIPTION

[0044] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. In the absence of conflict, the embodiments in this application and the features in the embodiments can be combined with each other. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0045] See also Figure 1-3 The present invention discloses an online microfluidic monitoring device, comprising:

[0046] A water pump, which is used to extract the water sample to be tested from the water source and transfer it to the water sample pool;

[0047] Water sample pool, used for temporary storage of water samples;

[0048] Reagent storage area, used to store test reagents;

[0049] A pipetting assembly, comprising a pipetting gun and a moving device, and used for transferring the water sample and the reagent;

[0050] A digestion component 5, the digestion component 5 includes a digestion bottle for receiving the water sample and the reagent, the digestion component 5 also includes a sealing device 7 and a heating device, the heating device is used to heat and digest the water sample and the reagent in the digestion bottle, and the sealing device 7 is used to seal the digestion bottle to prevent the reagent and water sample in the digestion bottle from evaporating during the digestion process;

[0051] A monitoring component is provided with a disc chip 1 and a puncturing mechanism 6, and the monitoring component is used to detect the digested reagents and water samples and to feed back the detection data in real time.

[0052] Through the above-mentioned structural setting, when the device is used, water samples can be extracted from the target water source at a fixed time and quantity, and after filtering the water samples, the samples are transferred to the corresponding digestion bottles in batches, and then the corresponding reagents are added to the corresponding digestion bottles and heated for digestion, and then the reagents are extracted and transferred to the disk chip 1 for detection, and the detection data is fed back in real time to achieve the effect of remote online monitoring;

[0053] After the disk chip 1 is inspected, further, the present application also includes a vacuum suction cup mechanism 8, a chip storage area 2 and a chip placement area 4;

[0054] The vacuum suction cup mechanism 8 is used to replace the used disk chip 1 in the monitoring component and transfer it to the chip placement area 4, and take out a new disk chip 1 from the chip storage area 2 and place it at the corresponding position of the monitoring component.

[0055] Through the above-mentioned structural arrangement, the vacuum suction cup mechanism 8 can transfer the disk chip 1 after the detection is completed, and transfer the new disk chip 1 to the corresponding area for subsequent re-detection.

[0056] It should be noted that, in the present application, a pneumatic assembly is also included, and the pneumatic assembly includes an air compressor, a pneumatic three-way valve and a vacuum generator;

[0057] The pneumatic assembly is used to provide negative pressure for the vacuum suction cup mechanism 8 to absorb the sealing device 7, the piercing mechanism 6 and the disc chip 1.

[0058] Furthermore, the vacuum suction cup mechanism 8 includes a movable slide rail 3 and a lifting seat 84 disposed on the movable slide rail 3;

[0059] The output end of the lifting seat 84 is provided with a chip suction cup 81 and a sealing suction cup 82 for adsorbing the sealing device 7, the piercing mechanism 6 and the disc chip 1;

[0060] The output end of the lifting seat 84 is also provided with an air guide tube 85 and a negative pressure tube 86, and the negative pressure tube 86 is used to connect the chip suction cup 81 and the sealing suction cup 82. The negative pressure tube 86 is provided with an adjustment member 83 at the center, and the adjustment member 83 is used to control the chip suction cup 81 or the sealing suction cup 82 to generate negative pressure;

[0061] The adjusting member 83 includes a driving unit and an air guide member 87 corresponding to the driving unit.

[0062] Through the above-mentioned structural setting, the pneumatic component can be used to provide negative pressure to the air guide tube 85 through the negative pressure tube 86, and through the adjustment of the air guide part 87, the chip suction cup 81 or the sealing suction cup 82 generates negative pressure, so as to facilitate the vacuum suction cup mechanism 8 to grasp the corresponding mechanism for transfer.

[0063] It should be added that the output end of the adjusting member 83 is fixedly connected to the air guide member 87. The air guide member 87 is a semi-conical partition for connecting with the negative pressure tube 86. Secondly, a partition is arranged between the air guide tubes 85 of the chip suction cup 81 and the sealing suction cup 82. The partition is combined with the air guide member 87 so that the negative pressure of the negative pressure tube 86 can only act on the chip suction cup 81 or the sealing suction cup 82. When in use, the adjusting member 83 can be rotated 180° to adjust the negative pressure of the chip suction cup 81 and the sealing suction cup 82.

[0064] In a preferred embodiment, the sealing device 7 and the piercing mechanism 6 are respectively arranged on both sides of the digestion component 5, and the digestion component 5, the sealing device 7 and the piercing mechanism 6 are sequentially arranged on one side of the movable slide rail 3 along the setting direction of the movable slide rail 3;

[0065] Correspondingly, the monitoring component, the chip storage area 2 and the chip placement area 4 are arranged in sequence on the other side of the movable slide rail 3 along the setting direction of the movable slide rail 3 .

[0066] The movable slide rail 3 can drive the lifting seat 84 and other corresponding structures disposed thereon to move along the movable slide rail 3;

[0067] The lifting seat 84 can drive the chip suction cup 81 and the sealing suction cup 82 arranged thereon to move up and down to complete corresponding actions such as adsorption, grasping, placement, and puncture.

[0068] The specific usage is that after the pipetting component transfers the water sample to the inside of the digestion component 5, it needs to be sealed to prevent the internal water sample from evaporating during the subsequent heating and digestion process. At this time, the movable slide rail 3 drives the sealing suction cup 82 to move to the sealing device 7. At the same time, the air guide 87 connects the sealing suction cup 82 with the negative pressure tube 86, so that the sealing suction cup 82 generates an adsorption force, and then the sealing suction cup 82 descends under the drive of the lifting seat 84, thereby adsorbing the corresponding sealing device 7, and then resets, and continues to be transferred to the corresponding digestion component 5 under the drive of the movable slide rail 3, and again descends under the drive of the lifting seat 84 to buckle on the digestion component 5 for sealing;

[0069] After the digestion is completed, in order to complete the addition of the reagent, the same movable slide rail 3 drives the sealing suction cup 82 to move to the puncture mechanism 6. At the same time, the air guide 87 connects the sealing suction cup 82 with the negative pressure tube 86, so that the sealing suction cup 82 generates an adsorption force, and then the sealing suction cup 82 is driven by the lifting seat 84 to descend, thereby adsorbing the corresponding puncture mechanism 6, and then reset, and continue to be driven by the movable slide rail 3 to move to the corresponding digestion component 5, and then descend again under the drive of the lifting seat 84, and puncture the sealing structure on the digestion component 5 through the puncture mechanism 6, so as to facilitate the subsequent addition and absorption of reagents;

[0070] In the process of adding the reagent, in a preferred embodiment, the liquid transfer component can first absorb the reagent, then absorb the water sample that has been digested in the decomposition component 5, and then transfer it to the disk chip 1 synchronously. In the above transfer process, the water sample and the reagent are mixed;

[0071] In another step that needs to be explained, after the corresponding disk chip 1 is used up, the same movable slide rail 3 drives the chip suction cup 81 to be transferred to the disk chip 1 in the monitoring component. At the same time, the air guide 87 connects the chip suction cup 81 with the negative pressure tube 86, so that the chip suction cup 81 generates an adsorption force, and then the chip suction cup 81 descends under the drive of the lifting seat 84, thereby adsorbing the corresponding disk chip 1, and then resets, and continues to be transferred to the corresponding chip placement area under the drive of the movable slide rail 3, and descends again under the drive of the lifting seat 84, and the used disk chip 1 is set in the chip placement area 4, and then, the movable slide rail 3 drives the chip suction cup 81 to move to the chip storage area 1, takes out the new disk chip 1 and transfers it to the monitoring component for subsequent monitoring.

[0072] In the present application, a lifting drive motor is provided in the chip storage area 2;

[0073] The lifting drive motor is used to control the height of the stacked disk chips 1 in the chip storage area 2, so that the vacuum suction cup mechanism 8 can transfer the used disk chips 1 to the chip storage area 2 and take out the new disk chips 1 at the corresponding position in the chip storage area 2.

[0074] It is important to add that in the present application, a control unit and a photoelectric detection unit are provided. The control unit is used to coordinate the operation of the above-mentioned process, and the photoelectric detection unit is used to identify the internal monitoring information of the disk chip 1 to obtain feedback. However, the above-mentioned structure and related technical features are commonly used technical means in the prior art, and therefore, they are not described and described in detail in the present application.

[0075] It should be noted that the number of digestion bottles is four groups;

[0076] The four groups of digestion bottles are respectively a COD digestion bottle 51, a TP digestion bottle 52, a TN digestion bottle 53 and an NH4H digestion bottle 54.

[0077] By setting corresponding digestion bottles, and different chemical indicators in each digestion bottle, after adding corresponding reagents, the disk chip 1 is used to detect various indicators of total nitrogen, ammonia nitrogen, COD and total phosphorus in the water sample.

[0078] The corresponding number of the pipetting components is also four, and they correspond one to one to the four groups of digestion bottles;

[0079] A flushing device is also provided in the pipetting assembly, and the flushing device is used to clean the pipette gun after the pipette gun absorbs the water sample or reagent, so as to avoid the mixing and contamination of the water sample and the reagent, which affects the subsequent detection accuracy.

[0080] Finally, it should be noted that a filtering device is provided in the water sample pool;

[0081] The filtering device is used to filter the water sample extracted by the water pump.

[0082] At the same time, the pneumatic three-way valve is connected to the output end of the filtering device;

[0083] Through the above-mentioned structural arrangement, the pneumatic three-way valve is used to introduce the gas generated by the air compressor into the filter device from the output end of the filter device to backwash the filter device.

[0084] The present invention adopts another technical solution. A method for using the online microfluidic monitoring device comprises the following steps:

[0085] S1. Extract water samples from the water source at regular intervals and in fixed quantities;

[0086] S2, after filtering the water sample, transfer the water sample to the COD digestion bottle 51, the TP digestion bottle 52, the TN digestion bottle 53 and the NH4H digestion bottle 54 respectively through a pipette gun and a mobile device for driving the transfer;

[0087] S3, rinse the pipette, and add the corresponding reagent into the corresponding digestion bottle through the pipette again;

[0088] S4, sealing the digestion bottle in step S3, and then heating and digesting;

[0089] S5, after the digestion is completed, the digested water sample mixture is finally transferred to the disc chip 1 by a pipette, and after the liquid capsule set on the disc chip 1 is punctured, a centrifugal test is performed;

[0090] S6. After the detection is completed, the used disk chip 1 is transferred to the chip storage area 2, and a new disk chip 1 is taken out from the chip storage area 2 and placed at the detection point for subsequent detection.

[0091] When in use, this method can be connected to an existing water source, and corresponding water samples can be taken out from the water source at a regular and quantitative manner. After digestion treatment, various indicators can be tested and data can be fed back to the terminal. The above process does not require human intervention and can be completed remotely and automatically, which effectively saves human resources and avoids errors in test results caused by human errors.

[0092] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0093] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0094] In addition, "plurality" means two or more.

[0095] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An online microfluidic monitoring device, characterized in that: include: A water pump, which is used to extract the water sample to be tested from the water source and transfer it to the water sample pool; Water sample pool, used for temporary storage of water samples; Reagent storage area, used to store test reagents; A pipetting assembly, comprising a pipetting gun and a moving device, and used for transferring the water sample and the reagent; A digestion component, the digestion component includes a digestion bottle for receiving the water sample and the reagent, the digestion component also includes a sealing device and a heating device, the heating device is used to heat and digest the water sample and the reagent in the digestion bottle, and the sealing device is used to seal the digestion bottle to prevent the reagent and water sample in the digestion bottle from evaporating during the digestion process; A monitoring component is provided with a disc chip and a puncturing mechanism, and the monitoring component is used to detect the digested reagents and water samples and to feed back the detection data in real time.

2. The online microfluidic monitoring device according to claim 1, characterized in that: It also includes a vacuum chuck mechanism, a chip storage area, and a chip placement area; The vacuum suction cup mechanism is used to replace the used disc chip in the monitoring component and transfer it to the chip placement area, and take out a new disc chip from the chip storage area.

3. The online microfluidic monitoring device according to claim 2, characterized in that: Also included is a pneumatic assembly, which includes an air compressor, a pneumatic three-way valve and a vacuum generator; The pneumatic assembly is used to drive the vacuum suction cup mechanism to absorb the sealing device, the puncturing mechanism and the disc chip.

4. The online microfluidic monitoring device according to claim 3, characterized in that: The vacuum suction cup mechanism includes a movable slide rail and a lifting seat arranged on the movable slide rail; The output end of the lifting seat is provided with a chip suction cup and a sealing suction cup for adsorbing the sealing device, the puncturing mechanism and the disc chip; The output end of the lifting seat is also provided with an air guide pipe and a negative pressure pipe, the negative pressure pipe is used to connect the chip suction cup and the sealing suction cup, and the negative pressure pipe is provided with an adjustment member in the middle, the adjustment member is used to control the chip suction cup or the sealing suction cup to generate negative pressure; The adjusting member includes a driving unit and an air guide member corresponding to the driving unit.

5. The online microfluidic monitoring device according to claim 2, characterized in that: The chip storage area and the chip placement area are provided with lifting drive motors; The lifting drive motor is used to control the height of the stacked disc chips in the chip storage area and the chip placement area, so that the vacuum suction cup mechanism can transfer the used disc chips to the chip placement area and take out the new disc chips at the corresponding position in the chip storage area.

6. The online microfluidic monitoring device according to claim 1, characterized in that: The number of the digestion bottles is four groups; The four groups of digestion bottles are respectively a COD digestion bottle, a TP digestion bottle, a TN digestion bottle and an NH4H digestion bottle.

7. The online microfluidic monitoring device according to claim 6, characterized in that: The number of the pipetting components is also four, and they correspond one to one to the four groups of digestion bottles; A flushing device is also provided in the pipetting assembly, and the flushing device is used to clean the pipette after the pipette absorbs the water sample or the reagent.

8. The online microfluidic monitoring device according to claim 3, characterized in that: A filtering device is provided in the water sample pool; The filtering device is used to filter the water sample extracted by the water pump.

9. The online microfluidic monitoring device according to claim 8, characterized in that: The pneumatic three-way valve is connected to the output end of the filtering device; The pneumatic three-way valve is used to introduce the gas generated by the air compressor into the filter device from the output end of the filter device to backwash the filter device.

10. The method for using the online microfluidic monitoring device according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1. Extract water samples from the water source at regular intervals and in fixed quantities; S2. After filtering the water sample, transfer the water sample to the COD digestion bottle, TP digestion bottle, TN digestion bottle and NH4H digestion bottle respectively through a pipette and a mobile device for driving the transfer; S3, rinse the pipette, and add the corresponding reagent into the corresponding digestion bottle through the pipette again; S4, sealing the digestion bottle in step S3, and then heating and digesting; S5. After the digestion is completed, the digested water sample mixture is finally transferred to the disc chip by a pipette, and after the liquid capsule set on the disc chip is punctured, a centrifugal test is performed; S6. After the test is completed, the used disk chip is transferred to the chip storage area, and a new disk chip is taken out from the chip storage area and placed at the test point for subsequent testing.

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