Method for correcting elbow of single-head cut-flower Chinese rose by using NPA

Abstract

The invention discloses a method for correcting a bend of a single-head cut-flower Chinese rose by using NPA, which is characterized in that auxin transport inhibitor NPA with different concentrations is applied to a bent part below a flower bud of a bent-stem plant at different development stages of the cut-flower Chinese rose, and phenotypic change and relieving of the bending degree of the bent-stem plant are observed. The result shows that the method can correct a certain bent stem angle, the longer the time of using 104 mol / L of NPA in the flower bud forming period is, the better the correction effect is, and the worst the bent stem correction effect of using 106 mol / L of NPA in the flower bud whitening period is. The method is easy and convenient to operate, economical and practical, the cost is reduced, the efficiency is improved, and the method can be used as a simple, convenient and practical bent stem correcting method.

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, in particular to a method for correcting the bend of a single-headed cut rose by utilizing NPA. Background Art

[0002] Cut rose (Rosa hybrida L.), also known as cut rose, is a perennial plant of the genus Rosa in the family Rosaceae. It usually has hooked prickles on the surface of its branches. The plant height is generally no more than two meters. It has five to seven leaflets and a well-developed root system. Because of its beautiful flowers, bright colors, rich fragrance, four-season blooming, and wide range of uses, it has a very high ornamental value and is deeply loved by people. It is the first of the four major fresh cut flowers in the world and is known as the "Queen of Flowers". Cut roses occupy an important position in the world's floriculture industry and international fresh cut flower trade. In recent years, the planting area of ​​cut roses in my country has exceeded 400,000 mu, and the annual output of fresh cut roses has reached more than 250 branches / m2, about 5 billion branches, and the annual output value has exceeded 20 billion yuan. Yunnan Province has the largest fresh cut flower planting area in China and is the world's largest fresh cut flower producing area. Cut roses account for 70% of Yunnan's fresh cut flower production. The main planting areas have gradually expanded from Kunming City to Qujing, Yuxi, Chuxiong, Honghe and other central Yunnan regions. In 2023, the Kunming Flower Auction Center announced that the output of cut roses in 2023 will be 1.1 billion branches, with an output value of 1.3 billion yuan.

[0003] The quality of cut roses depends on their flower shape, color and fragrance. However, an unsuitable growth environment can cause abnormal development of rose plants, resulting in flower deformities, physiological diseases of cut roses, and seriously affecting their ornamental quality and commercial value. Based on many domestic and foreign research results, several common physiological diseases of cut roses were sorted out, such as leaf rot, ox-heart buds and double heart flowers, and bent stems. Bent stem phenomenon (BPP) is common in cut roses during cultivation. BPP is not a neck bending phenomenon caused by water stress after harvest, but a physiological deformity. The incidence varies from variety to variety, such as 10% to 30% for single-headed variety Mercedes, 10% to 20% for Beast, about 20% for Corolla, and 15% to 25% for Peach Snow Mountain. The occurrence of bent stems leads to the production of deformed flowers, which seriously affects the ornamental quality of roses, reduces sales and thus reduces economic benefits. BPP often occurs in cultivation, but people are accustomed to this phenomenon and have not conducted systematic research or taken corresponding measures to prevent the occurrence of bent stems. Summary of the invention

[0004] The purpose of the present invention is to provide a method for correcting the bend of a single-headed cut rose using NPA, which can effectively correct the bent stems of the cut roses and provide more options for correcting the bent stems.

[0005] The above technical purpose of the present invention is achieved through the following technical scheme: a method for correcting the bend of a single-headed cut rose using NPA, using 1-N-naphthylphthalic acid solution to smear the curved part below the flower bud of a curved-stem plant during the development stage of the cut rose.

[0006] Further preferably, the concentration of the 1-N-naphthylphthalic acid solution is 10 6 -10 4 mol / L.

[0007] Further preferably, the concentration of the 1-N-naphthylphthalic acid solution is 10 4 mol / L.

[0008] Further preferably, the concentration of the 1-N-naphthylphthalic acid solution is 10 5 mol / L.

[0009] Further preferably, the concentration of the 1-N-naphthylphthalic acid solution is 10 6 mol / L.

[0010] Further preferably, the developmental stage of the cut rose is the bud stage.

[0011] Further preferably, the development stage of the cut rose is the bud formation stage.

[0012] Further preferably, the development stage of the cut rose is the bud development stage.

[0013] Further preferably, the developmental stage of the cut rose is the bud whitening stage.

[0014] In summary, the present invention has the following beneficial effects: the present invention uses three concentrations of exogenous auxin transport inhibitor NPA to apply to the curved part below the curved stalk buds at different developmental stages of roses, and screens out a more suitable concentration (10 4 mol / L) and the developmental stage (bud formation stage) of rose stem bending, and it was found that NPA had a good effect on correcting the angle and degree of rose stem bending. This method is simple, convenient, economical and practical, which not only reduces costs but also improves efficiency. It can be used as a simple and practical method to correct rose stem bending. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is the different developmental stages of the single-headed cut rose Gaosheng curved stem plant;

[0016] Figure 2 is the phenotypic changes of bent-stem plants at different developmental stages before and after NPA treatment (3 days);

[0017] Figure 3The straightening rate and significance of bent-stem plants at different developmental stages treated with NPA at different concentrations;

[0018] Figure 4 It is the change of the bending stem angle before and after (1d and 3d) different developmental stages of the bent-stem plants treated with different concentrations. DETAILED DESCRIPTION

[0019] In order to make those skilled in the art better understand the technical scheme of the present invention, the present invention is further described in detail below in conjunction with embodiments. It will be understood by those skilled in the art that the following embodiments are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention.

[0020] Example

[0021] 1 Materials and methods

[0022] 1.1 Test materials

[0023] The experimental material was single-headed 'Golden Sheng' rose (Rosa hybrida 'Gotcha'), planted in a cut rose greenhouse in Yao'an County, Chuxiong Prefecture, Yunnan Province, and cultivated and managed conventionally. Plants with healthy growth, no diseases or insect pests, and bent stems and deformities at different development stages (bud formation, bud development, and bud whitening) were selected ( Figure 1 ) and tested the exogenous auxin transport inhibitor NPA.

[0024] 1.2 Main Reagents

[0025] Test reagent: 1-N-naphthylphthalamic acid (NPA), commonly known as naphthamide, is in powder form and was purchased from Shanghai MacLean Biochemical Technology Co., Ltd. and stored at room temperature.

[0026] 1.3 Test methods

[0027] From 8:00 am to 12:00 pm, select Goldman Sachs' three development stages ( Figure 1 ) was used for the experiment. The experimental agent was the auxin transport inhibitor NPA. The concentration was adjusted appropriately based on the previous research. A single-factor completely randomized experimental design was used, with CK (pure water), 10 4 mol / L、10 5 mol / L、10 6 mol / L, a total of 4 different NPA concentrations. NPA was applied to the curved pedicels below the flower buds at different developmental stages. Fifteen curved-stem plants were treated with each concentration, and each treatment was repeated three times. Each treatment was recorded with a tag marked with concentration, developmental stage, and time. The degree of bending and the angle of the curved-stem plants were observed 1 day and 3 days after treatment. The phenotype of the curved-stem plants was photographed using a photo box, and the angle of the curved stem was measured with a protractor.

[0028] 1.4 Data Processing

[0029] PowerPoint software was used to process images, and Excel 2019 was used for data statistics and organization. SPSS26.0 was used for data processing and difference analysis, with P < 0.05 as a significant difference and P < 0.01 as an extremely significant difference. The Duncan range method was used for multiple comparisons of means. Origin 2021 software was used to draw bar graphs.

[0030] 2 Results and analysis

[0031] 2.1 Phenotypic changes of cut rose curved-stem plants at different developmental stages before and after NPA treatment

[0032] In order to observe whether the exogenous auxin transport inhibitor NPA has a straightening effect on the curved stems of 'Gaosheng' rose plants, roses at three different developmental stages were treated with different concentrations of NPA. The phenotypic changes of roses were observed. Figure 2 It can be seen that the curved stem plants have leaf changes and sepalization. One mutant sepal is similar to a leaf, falls and develops on the pedicel below the bud. The location of the mutant sepal becomes flat and the diameter becomes larger, and the direction is consistent with the bending direction of the pedicel. Before NPA treatment, the curved stem plants were more curved, but 3 days after NPA treatment, it was observed that the curvature of the curved stems at different development stages of roses was alleviated.

[0033] 2.2 Statistics of straightening rates of cut rose plants with curved stems at different stages under different NPA concentration treatments

[0034] To observe the effect of NPA on straightening bent-stem plants, different concentrations of NPA were applied to the bent peduncles of plants at different developmental stages. Figure 3 As can be seen from Table 1, the straightening rate of the bent-stem plants was 0.00% when CK (pure water) was applied, indicating that pure water had no straightening effect on the bent-stem plants. 4 mol / L NPA 3d and the other two concentrations of 10 5 , 10 6 mol / L had significant difference (P<0.05), and the straightening rate was as high as 81.67%, indicating that 4 mol / L treatment of bent stems had obvious straightening effect 3 days after treatment. The three concentrations of treatment at the bud development stage (St2) had similar effects 1 day and 3 days after treatment. 6The straightening rate of 1 d and 3 d after treatment with 10 mol / L NPA was significantly different from that of the other two concentrations (P < 0.05). The straightening rates of 1 d and 3 d were both 40.00%, indicating that the higher the NPA concentration, the better the straightening effect. 5 mol / L treatment at 3 days was significantly different from that at other concentrations at other developmental stages (P < 0.05), with a straightening rate of 61.67%. 6 The straightening effect was the worst at the budding stage (CB), with the straightening rates of 5.00% at 1d and 3d. The above results show that the straightening effect of 10-4mol / L was the best at 3d after treatment at the bud formation stage (St1).

[0035] in Figure 3 In the table, 1, 2, 3, and 4 represent the CK of the first day, 10 4 , 10 5 , 10 6 mol / L; 5, 6, 7, 8 represent CK on the third day, 10 4 , 10 5 , 10 6 mol / L, the same below.

[0036] Table 1 The straightening rate of curved stems of 'Gaosheng' cut rose plants at different developmental stages treated with different NPA concentrations

[0037]

[0038] 2.3 Changes in the bending angle of cut rose plants at different stages under different NPA concentrations

[0039] In order to observe the degree of plant bending relief by different NPA concentrations, the angle of the bent stem of plants before and after treatment at different developmental stages (1d and 3d) was measured with a protractor. Figure 4 As shown in Table 2, 10 4 3 days after the application of 10-6 mol / L NPA, the bending degree of the plants was alleviated to the greatest extent, which was 6.6°. The effect of using 10-6 mol / L in the CB period to alleviate the bending degree was the worst, which was only 0.4°. It can be seen that the use of NPA has a certain ability to alleviate the bending angle, and 10 4 The longer the treatment time of NPA in St1 was, the better the mitigation effect was.

[0040] in Figure 4 In the middle, 1 represents 10 4 mol / L; 2, represents 10 5 mol / L; 3, represents 106 mol / L. Dark gray represents the bending angle before St1 is treated with NPA, red represents the bending angle 1 day after St1 is treated with NPA, and dark blue represents the bending angle 3 days after St1 is treated with NPA; green represents the bending angle before St2 is treated with NPA, purple represents the bending angle 1 day after St2 is treated with NPA, and light yellow represents the bending angle 3 days after St2 is treated with NPA; light blue represents the bending angle before CB is treated with NPA, dark purple represents the bending angle 1 day after CB is treated with NPA, and dark yellow represents the bending angle 3 days after CB is treated with NPA.

[0041] Table 2 Changes in the bending angle of the bent-stem plants before and after (1d and 3d) different developmental stages under different NPA concentration treatments

[0042]

[0043] 3 Conclusion

[0044] The auxin of roses mainly comes from flower organs. Previous studies have shown that the appearance of curved stems of cut roses is related to auxin. Exogenous hormones NAA and IAA cause normal plants to temporarily bend their stems. 1-N-Naphthylphthalamic acid (NPA), commonly known as naphthamide, is a key inhibitor of polar auxin transport in plants. Therefore, the present invention uses three concentrations of exogenous auxin transport inhibitor NPA to apply to the curved parts below the curved stem buds of roses at different developmental stages, and screens out a more suitable concentration (10 4 mol / L) and the developmental stage (bud formation stage) of rose stem bending, and it was found that NPA had a good effect on correcting the angle and degree of rose stem bending. This method is simple, convenient, economical and practical, which not only reduces costs but also improves efficiency. It can be used as a simple and practical method to correct rose stem bending.

[0045] The above embodiments are merely explanations of the present invention and are not limitations of the present invention. After reading this specification, those skilled in the art may make modifications to the embodiments without any creative contribution as needed. However, such modifications are protected by the patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A method for correcting the elbow of a single-head cut rose using NPA, characterized in that: Use 1-N-naphthylphthalic acid solution to apply the curved part below the flower buds of curved-stemmed plants during the development stage of cut roses.

2. The method for correcting the elbow of a single-head cut rose using NPA according to claim 1, characterized in that: The concentration of the 1-N-naphthylphthalic acid solution is 10 6 -10 4 mol / L.

3. A method for correcting the elbow of a single-head cut rose using NPA according to claim 2, characterized in that: The concentration of the 1-N-naphthylphthalic acid solution is 10 4 mol / L.

4. The method for correcting the elbow of a single-head cut rose using NPA according to claim 2, characterized in that: The concentration of the 1-N-naphthylphthalic acid solution is 10 5 mol / L.

5. The method for correcting the elbow of a single-head cut rose using NPA according to claim 2, characterized in that: The concentration of the 1-N-naphthylphthalic acid solution is 10 6 mol / L.

6. A method for correcting the elbow of a single-head cut rose using NPA according to any one of claims 1 to 5, characterized in that: The development stage of the cut rose is the bud stage.

7. The method for correcting the elbow of a single-head cut rose using NPA according to claim 6, characterized in that: The development stage of the cut rose is the bud formation stage.

8. The method for correcting the elbow of a single-head cut rose using NPA according to claim 6, characterized in that: The development stage of the cut rose is the bud development stage.

9. The method for correcting the elbow of a single-head cut rose using NPA according to claim 6, characterized in that: The development stage of the cut rose is the stage when the flower buds turn white.

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