Method for increasing content of andrographis paniculata diterpene lactone substances through ammonium ions

Abstract

The invention discloses a method for increasing the content of andrographis paniculata diterpene lactone substances through ammonium ions, which comprises the following steps: a nutrient solution is used for irrigating matrix soil for culture, and the matrix soil culture nutrient solution comprises 1 mmol. L <-1 > of NaH2PO3, 1 mmol. L <-1 > of KCl, 1 mmol. L <-1 > of MgSO4, 2 mmol. L <-1 > of CaCl2, 36 [mu] mol. L <-1 > of FeCl3, 9.5 [mu] mol. L <-1 > of MnSO4, 0.15 [mu] mol. L <-1 > of ZnSO4, 0.15 [mu] mol. L <-1 > of CuSO4, 0.52 [mu] mol. L <-1 > of (NH4) 6Mo24, 18 [mu] mol. L <-1 > According to the invention, the content of andrographolide and dehydroandrographolide is obviously increased.

Description

Technical Field

[0001] The invention belongs to the technical field of andrographolide cultivation, and particularly relates to a method for increasing andrographolide diterpenoid lactone substances by using ammonium ions. Background Art

[0002] Andrographis paniculata (Burm. f.) Nees is an annual herbaceous medicinal plant widely distributed in tropical and subtropical regions of Asia, and has been widely used in medicine all over the world. It contains rich diterpene lactone components with anti-tumor, anti-inflammatory, antiviral activities and the potential to treat liver and lung damage. Among them, andrographolide, neoandrographolide, 14-deoxyandrographolide and dehydroandrographolide are the main diterpene lactone components. Modern pharmacological studies have shown that diterpene compounds represented by andrographolide are the main pharmacological substances that exert the pharmacological activity of Andrographis paniculata.

[0003] Nitrogen is the mineral nutrient element with the largest demand in plants. It is an important component of small molecules such as plant proteins, amino acids, chlorophyll, nucleic acids, and some secondary metabolites with special functions. It affects important metabolic processes such as plant growth and development, yield quality formation, and reproduction. Insufficient supply will have an adverse effect on plant growth and development and secondary metabolic processes. Nitrogen is generally lacking in soil under natural conditions. Increasing the application of nitrogen fertilizer to increase crop yield is the most commonly used means and method in agricultural production. However, the large-scale application of nitrogen fertilizer will not only reduce the quality of medicinal plants, but also bring a series of environmental and ecological problems to the sustainable development of agriculture. In order to improve the quality of medicinal plants, cultivation methods such as ecological planting and bionic planting that reduce the application of nitrogen fertilizer have been paid attention to. However, while reducing the application of nitrogen fertilizer can improve the quality of medicinal materials, it will also limit their yield or cause the harvest time of medicinal materials to be delayed, making it difficult to meet the needs of the market and industry.

[0004] Therefore, there is an urgent need for a method that can balance the growth of Andrographis paniculata, meet the demand for Andrographis paniculata medicinal material production, and improve the quality of Andrographis paniculata. Summary of the invention

[0005] An object of the present invention is to solve at least the above-mentioned drawbacks and to provide advantages which will be described hereinafter.

[0006] In order to achieve these purposes and other advantages of the present invention, a method for increasing the production of diterpene lactones in Andrographis paniculata by using ammonium ions is provided, comprising: watering and cultivating with a nutrient solution for at least 30 days, watering once every 7 days, and watering once with 100 ml.

[0007] The nutrient solution included 1 mmol·L -1 NaH 2 PO 3 , 1 mmol·L-1 KCl, 1 mmol·L -1 MgSO 4 , 2mmol·L -1 CaCl 2 , 36 μmol·L -1 FeCl 3 , 9.5 μmol·L -1 MnSO 4 , 0.15 μmol·L -1 ZnSO 4 , 0.15 μmol·L -1 CuSO 4 , 0.52 μmol·L -1 (NH 4 ) 6 Mo 24 , 18 μmol·L -1 H 3 BO 3 and 0.2 mmol·L -1 ~6 mmol·L -1 NH 4 + ; pH of nutrient solution = 6.0.

[0008] In the above scheme, the nutrient solution is adjusted and the cultivation management is adjusted so that Andrographis paniculata can achieve better growth and significantly increase the content of andrographolide and dehydroandrographolide.

[0009] Preferably, the conditions for watering and cultivating the Andrographis paniculata seedlings are: The method for increasing the content of andrographolide lactones by ammonium ions comprises: Take andrographis paniculata seeds and sow them to obtain white andrographis paniculata seed buds; Prepare nutrient solution, which includes 1 mmol·L -1 NaH 2 PO 3 , 1 mmol·L -1 KCl, 1 mmol·L -1 MgSO 4 , 2 mmol·L -1 CaCl 2 , 36 μmol·L -1 FeCl 3 , 9.5 μmol·L -1 MnSO 4 , 0.15 μmol·L -1 ZnSO 4 , 0.15 μmol·L -1 CuSO4 , 0.52 μmol·L -1 (NH 4 ) 6 Mo 24 , 18 μmol·L -1 H 3 BO 3 and 1 mmol·L -1 NH 4 + ~6mmol·L -1 NH 4 + ; pH of nutrient solution = 6.0.

[0010] The whitened Andrographis paniculata seed buds are placed in a seedling tray and grown for 10 days, and then transplanted into a seedling hole tray and grown until they grow into a pair of true leaves and one heart, thereby obtaining Andrographis paniculata seedlings.

[0011] The Andrographis paniculata seedlings were transplanted into brown culture pots again and irrigated with nutrient solution for at least 30 days, irrigated once every 7 days, 100 ml each time.

[0012] Preferably, the culture matrix of the seedling tray is vermiculite: peat soil = 3:1; the culture matrix of the brown culture pot is vermiculite: perlite = 3:1.

[0013] Preferably, the brown culture pot has a diameter to height ratio of 12:11.

[0014] Preferably, the method of increasing the content of diterpene lactones of Andrographis paniculata by ammonium ions further comprises watering with deionized water to keep the plants moist for the first 4 days after transplanting them into the brown culture pots, and watering with nutrient solution 4 days later.

[0015] The method for increasing the content of andrographolide lactones by ammonium ions comprises: Take Andrographis paniculata seeds and sow them to obtain white Andrographis paniculata seed sprouts.

[0016] Prepare nutrient solution, which includes 1 mmol·L -1 NaH 2 PO 3 , 1 mmol·L -1 KCl, 1 mmol·L -1 MgSO 4 , 2 mmol·L -1 CaCl 2 , 36 μmol·L -1 FeCl 3 , 9.5 μmol·L -1 MnSO 4 , 0.15 μmol·L-1 ZnSO 4 , 0.15 μmol·L -1 CuSO 4 , 0.52 μmol·L -1 (NH 4 ) 6 Mo 24 , 18 μmol·L -1 H 3 BO 3 and 0.2 mmol·L -1 NH 4 + ~6mmol·L -1 NH 4 + ; pH of nutrient solution = 6.0.

[0017] Place the white Andrographis paniculata seed buds in a seedling tray and grow them for 10 days. When they grow into a pair of true leaves and one heart, transfer them to a nutrient solution for hydroponics for at least 30 days, and replace the nutrient solution every 7 days.

[0018] Advantages of the present invention: Firstly, the present invention improves the composition of the nutrient solution, thereby obtaining a method capable of balancing the growth of andrographis paniculata and improving the quality of andrographis paniculata.

[0019] Secondly, the method of the present invention significantly increases the content of andrographolide and dehydroandrographolide. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 1 mmol·L -1 KNO 3 With NH 4 Cl phenotype comparison; Figure 2 3 mmol·L -1 KNO 3 With NH 4 Cl phenotype comparison; Figure 3 6mmol·L -1 KNO 3 With NH 4 Cl phenotype comparison; Figure 4 6mmol·L -1 KNO 3 With (NH 4 ) 2 SO 4 Phenotypic comparison; Figure 5 6mmol·L-1 KNO 3 With CH 3 COONH 4 Phenotypic comparison; Figure 6 6mmol·L -1 KNO 3 With NH 4 HCO 3 Phenotypic comparison. DETAILED DESCRIPTION

[0021] The present invention is described in further detail below so that those skilled in the art can implement it according to the description.

[0022] Example 1 The method for increasing andrographolide diterpenoids by ammonium ions comprises the following steps: Step 1: Sow Andrographis paniculata seeds in a seedling tray, water them sufficiently and cover them with plastic wrap, and remove the plastic wrap after the seeds turn white on the third day to obtain the white Andrographis paniculata seed buds; wherein the culture medium of the seedling tray is vermiculite.

[0023] Step 2: Place the whitened Andrographis paniculata seed buds in a seedling tray and grow them for 10 days. Then transplant them into a seedling hole tray and grow them for 30 days until they grow into a pair of true leaves and one heart, thereby obtaining Andrographis paniculata seedlings.

[0024] Step 3: Take 1 mmol·L -1 NaH 2 PO 3 , 1 mmol·L -1 KCl, 1 mmol·L -1 MgSO 4 , 2 mmol·L - 1 CaCl 2 , 36 μmol·L -1 FeCl 3 , 9.5 μmol·L -1 MnSO 4 , 0.15 μmol·L -1 ZnSO 4 , 0.15 μmol·L - 1 CuSO 4 , 0.52 μmol·L -1 (NH 4 ) 6 Mo 24 , 18 μmol·L -1 H 3 BO 3 and 1 mmol·L-1 NH 4 Cl mixed to obtain nutrient solution.

[0025] Step 4: Transplant the Andrographis paniculata seedlings into brown culture pots and water them with nutrient solution for at least 30 days, watering them once every 7 days, 100 ml each time. The culture conditions are: a 14-hour light cycle, lights on at 7 a.m. and off at 9 p.m., LED light source, and light intensity of 200 μmol m -2 ⋅s -1 The indoor temperature is 28℃ during the day and 25℃ at night.

[0026] Comparative Example 1 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 1mmol·L -1 KNO 3 .

[0027] Example 2 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 3mmol·L -1 NH 4 Cl.

[0028] Comparative Example 2 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 3mmol·L -1 KNO 3 .

[0029] Example 3 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 NH 4 Cl.

[0030] Comparative Example 3 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 KNO 3 .

[0031] Example 4 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 (NH 4 ) 2 SO 4 .

[0032] Comparative Example 4 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 KNO 3 .

[0033] Example 5 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 CH 3 COONH 4 .

[0034] Comparative Example 5 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 KNO 3 .

[0035] Example 6 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 NH 4 HCO 3 .

[0036] Comparative Example 6 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 1, except that the composition of the nutrient solution in Step 3 is 1 mmol·L -1 NH 4 Cl is replaced by 6mmol·L -1 KNO 3 .

[0037] Example 7 The method for increasing andrographolide diterpenoids by ammonium ions comprises the following steps: Step 1: Sow Andrographis paniculata seeds in a seedling tray, water them sufficiently and cover them with plastic wrap, and remove the plastic wrap after the seeds turn white on the third day to obtain the white Andrographis paniculata seed buds; wherein the culture medium of the seedling tray is vermiculite.

[0038] Step 2: Place the white Andrographis paniculata seed buds in a seedling tray and grow them for 10 days. Then transplant them into the nutrient solution and fix them with a sponge for hydroponic culture. The specifications of the hydroponic box are 12cm×12cm×12cm, and 4 plants are cultivated in each box.

[0039] Step 3: Take 1 mmol·L -1 NaH 2 PO 3 , 1 mmol·L -1 KCl, 1 mmol·L -1 MgSO 4 , 2 mmol· L- 1 CaCl 2 , 36 μmol·L -1 FeCl 3 , 9.5 μmol·L -1 MnSO 4 , 0.15 μmol·L -1 ZnSO 4 , 0.15 μmol·L - 1 CuSO 4 , 0.52 μmol·L -1 (NH4 ) 6 Mo 24 , 18 μmol·L -1 H 3 BO 3 and 0.05mmol·L -1 NH 4 Cl mixed to obtain nutrient solution.

[0040] Step 4: Cultivate with nutrient solution water for at least 30 days from the seedling stage, and replace the nutrient solution every 7 days. The cultivation conditions are: 14-hour light cycle, lights on at 7 am and off at 21 pm, LED light source, light intensity of 200 μmol / m -2 ⋅s -1 The indoor temperature is 28℃ during the day and 25℃ at night.

[0041] Comparative Example 7 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 0.05mmol·L -1 KNO 3 .

[0042] Example 8 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 0.1mmol·L -1 NH 4 Cl.

[0043] Comparative Example 8 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 0.1mmol·L -1 KNO 3 .

[0044] Example 9 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1NH 4 Cl was replaced with 0.2mmol·L -1 NH 4 Cl.

[0045] Comparative Example 9 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 0.2mmol·L -1 KNO 3 .

[0046] Example 10 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 0.5mmol·L -1 NH 4 Cl.

[0047] Comparative Example 10 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 0.5mmol·L -1 KNO 3 .

[0048] Embodiment 11 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl is replaced by 1mmol·L -1 NH 4 Cl.

[0049] Comparative Example 11 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl is replaced by 1mmol·L -1 KNO 3 .

[0050] Example 12 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 2mmol·L -1 NH 4 Cl.

[0051] Comparative Example 12 The method for increasing the amount of andrographolide lactones by ammonium ions comprises the following steps: Step 1, Step 2, and Step 4 are the same as those in Example 7, except that the composition of the nutrient solution in Step 3 is 0.05 mmol·L -1 NH 4 Cl was replaced with 2mmol·L -1 KNO 3 .

[0052] Determination of the content of diterpenoid lactones in Andrographis paniculata The Andrographis paniculata plants were obtained by culturing according to the methods of Examples 1 to 12 and Comparative Examples 1 to 12, and the growth of each group of plants was observed and recorded. The results are as follows: Figure 1 ; Then the contents of andrographolide, neoandrographolide, 14-deoxyandrographolide, dehydroandrographolide and total andrographolide were determined respectively, and the results are shown in Table 1; the plant biomass and total nitrogen content were determined, and the results are shown in Table 2.

[0053] Table 1: Content of diterpenoid lactones in soil culture (mg·g 1 ) project Andrographolide Increase percentage % Neoandrographolide Increase percentage % 14-Deoxyandrographolide Increase percentage % Dehydroandrographolide Increase percentage % Total andrographolide Increase percentage % Comparative Example 1 27.51 / 7.28 / 52.48 / 11.40 / 98.66 / Example 1 30.69 11.55 6.71 -7.74 50.24 -4.25 10.45 -8.36 98.09 -0.58 Comparative Example 2 15.32 / 5.47 / 55.97 / 1.97 / 81.69 / Example 2 20.73 35.30 5.80 5.94 53.00 -5.30 9.36 374.44 87.73 7.39 Comparative Example 3 10.92 / 2.63 / 32.99 / 1.06 / 48.03 / Example 3 22.26 75.47 5.09 3.40 56.13 -1.10 11.20 370.03 94.68 15.98 Comparative Example 4 10.92 / 2.63 / 32.99 / 1.06 / 48.03 / Example 4 20.93 91.61 2.96 12.39 38.09 15.49 3.60 238.88 64.03 33.32 Comparative Example 5 10.92 / 2.63 / 32.99 / 1.06 / 48.03 / Example 5 14.43 32.07 2.95 12.11 38.32 16.18 1.87 76.22 57.57 19.86 Comparative Example 6 10.92 / 2.63 / 32.99 / 1.06 / 48.03 / Example 6 13.37 22.41 2.71 2.89 37.27 12.98 2.14 100.78 55.48 15.51 Table 2: Biomass of Andrographis paniculata under soil culture mode (g·plant -1 ) and total nitrogen content (mg·g 1 ) pass Figure 1-3 It can be seen that with the increase of nitrogen concentration, the growth of Andrographis paniculata is also better, 6mmol·L -1 Ammonium salts will not harm the growth of Andrographis paniculata.

[0054] According to the data in Table 1-2, when the concentration reaches 6 mmol·L -1 When KNO 3 The biomass of the treatment was significantly higher than that of NH 4 The Cl treatment was 21.63% higher than the latter, and there was no significant difference between the other two concentrations; 4The total nitrogen content of plants treated with Cl was significantly higher than that of plants treated with KNO 3 The total andrographolide content was also higher when the nitrogen concentration reached 6mmol·L -1 When NH 4 The Cl treatment was significantly higher than KNO 3 treatment, which was 15.98% higher; and when the nitrogen concentration was greater than 3mmol·L -1 When NH 4 Cl treatment significantly increased the contents of andrographolide and dehydroandrographolide. -1 and 6mmol·L -1 and 35.30% and 75.47% respectively; and dehydroandrographolide increased by 374.44% and 370.03% respectively.

[0055] Depend on Figure 4-6 From Table 1-2, we can see that KNO 3 Compared with the treatments, after 30 days of treatment with different ammonium salt types at the same concentration, there was no difference in biomass, and the total nitrogen content was only CH 3 COONH 4 The contents of total andrographolide, andrographolide, 14-deoxyandrographolide and dehydroandrographolide in the ammonium ion treatment were significantly increased. (NH 4 ) 2 SO 4 , CH 3 COONH 4 NH 4 HCO 3 The total lactone contents of KNO 3 treatments were 33.32%, 19.86% and 15.51% higher than those in KNO 3 treatments were 91.61%, 32.07% and 22.41% higher than those in KNO 3 treatments were 15.49%, 16.18% and 12.98% higher than those in KNO 3 The treatments were 238.88%, 76.22% and 100.78% higher.

[0056] Table 3: Content of diterpenoid lactones in andrographolide in hydroponic culture (mg·g 1 ) project Andrographolide Increase percentage % Neoandrographolide Increase percentage % 14-Deoxyandrographolide Increase percentage % Dehydroandrographolide Increase percentage % Total andrographolide Increase percentage % Comparative Example 7 20.33 / 3.62 / 26.61 / 1.49 / 52.04 / Example 7 22.08 8.60 3.32 -8.31 27.46 3.21 0.57 -61.34 54.02 3.80 Comparative Example 8 18.60 / 3.17 / 32.32 / 1.27 / 55.36 / Example 8 19.66 5.71 3.27 3.23 31.50 -2.56 0.54 -57.52 54.97 -0.70 Comparative Example 9 18.17 / 3.28 / 32.91 / 1.60 / 55.96 / Example 9 18.77 3.29 3.06 -6.82 40.87 24.18 0.67 -58.13 63.36 13.22 Comparative Example 10 17.72 / 2.99 / 36.29 / 1.32 / 58.33 / Example 10 18.96 7.02 3.25 8.59 40.30 11.04 0.70 -47.06 63.22 8.36 Comparative Example 11 8.62 / 2.23 / 38.45 / 0.48 / 49.79 / Embodiment 11 13.49 56.43 1.96 -11.93 41.30 7.40 1.25 157.89 58.00 16.48 Comparative Example 12 4.35 / 1.39 / 34.61 / 0.25 / 40.61 / Example 12 17.69 306.51 1.43 2.46 24.7.3 -28.55 3.83 1412.42 47.68 17.40 As shown in Table 3, under the hydroponic mode, the total lactone content in the single NH 4 + Concentration up to 0.2 mmol·L -1The above showed obvious advantages compared with NO alone 3 - The comparison reached a significant level in NH 4 + The concentration is 0.2 mmol·L -1 , 0.5 mmol·L -1 , 1 mmol·L -1 , 2 mmol·L -1 NO 3 - The results exceeded 13.22%, 8.38%, 16.48% and 17.40%. 4 + The content of andrographolide and dehydroandrographolide was significantly affected. The content of andrographolide in NH 4 + The concentration is 1 mmol·L -1 and 2 mmol·L -1 NO 3 - exceeded 56.43% and 306.51%; the content of dehydroandrographolide in NH 4 + The concentration is 1 mmol·L -1 and 2 mmol·L -1 Compared with NO 3 - Exceeded 157.89% and 1412.42%.

[0057] Although the embodiments of the present invention have been disclosed above, they are not limited to the applications listed in the description and the embodiments. It can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily realized. Therefore, without departing from the general concept defined by the claims and equivalent scope, the present invention is not limited to specific details.

Claims

1. A method for increasing andrographolide lactones by ammonium ions, comprising: The Andrographis paniculata seedlings were watered with nutrient solution for at least 30 days, once every 7 days, with 100 ml each time; The nutrient solution included 1 mmol·L -1 NaH2PO3, 1 mmol·L -1 KCl, 1 mmol·L -1 MgSO4, 2 mmol·L -1 CaCl2, 36 μmol·L -1 FeCl3, 9.5 μmol·L -1 MnSO4, 0.15 μmol·L -1 ZnSO4, 0.15 μmol·L -1 CuSO4, 0.52 μmol·L -1 (NH4)6Mo 24 , 18 μmol·L -1 H3BO3 and 0.2 mmol·L -1 ~6 mmol·L - 1 NH4 + ; pH of nutrient solution = 6.

0.

2. The method for increasing the content of andrographolide lactones by ammonium ions according to claim 1, characterized in that: The conditions for watering and cultivating Andrographis paniculata seedlings were as follows: a 14-hour photoperiod, lights on at 7 a.m. and off at 21 p.m., and a light intensity of 200 μmol m -2 ⋅s -1 The indoor temperature is 28℃ during the day and 25℃ at night, and the humidity is 80%.

3. The method for increasing the content of andrographolide lactones by ammonium ions according to claim 1, characterized in that: include: Take andrographis paniculata seeds and sow them to obtain white andrographis paniculata seed buds; Prepare nutrient solution, which includes 1 mmol·L -1 NaH2PO3, 1 mmol·L -1 KCl, 1 mmol·L -1 MgSO4, 2mmol·L -1 CaCl2, 36 μmol·L -1 FeCl3, 9.5 μmol·L -1 MnSO4, 0.15 μmol·L -1 ZnSO4, 0.15 μmol·L -1 CuSO4, 0.52 μmol·L -1 (NH4)6Mo 24 , 18 μmol·L -1 H3BO3 and 1 mmol·L -1 NH4 + ~6mmol·L -1 NH4 + ; pH of nutrient solution = 6.0; The whitened Andrographis paniculata seed buds are placed in a seedling tray and grown for 10 days, and then transplanted into a seedling hole tray and grown until they have a pair of true leaves and one heart, thereby obtaining Andrographis paniculata seedlings; The Andrographis paniculata seedlings were transplanted into brown culture pots again and irrigated with nutrient solution for at least 30 days, irrigated once every 7 days, 100 ml each time.

4. The method for increasing the content of andrographolide lactones by ammonium ions according to claim 2, characterized in that: The culture substrate of the seedling hole tray is vermiculite: peat soil = 3:1; the culture substrate of the brown culture pot is vermiculite: perlite = 3:

1.

5. The method for increasing andrographolide lactones by ammonium ions according to claim 2, characterized in that: The brown culture pot has a diameter-to-height ratio of 12:

11.

6. The method for increasing the content of andrographolide lactones by ammonium ions according to claim 2, characterized in that: After transplanting into the brown culture pot, deionized water was used for watering to keep it moist for the first 4 days, and then nutrient solution was used for watering and cultivation after 4 days.

7. The method for increasing the content of andrographolide lactones by ammonium ions according to claim 2, characterized in that: include: Take andrographis paniculata seeds and sow them to obtain white andrographis paniculata seed buds; Prepare nutrient solution, which includes 1 mmol·L -1 NaH2PO3, 1 mmol·L -1 KCl, 1 mmol·L -1 MgSO4, 2mmol·L -1 CaCl2, 36 μmol·L -1 FeCl3, 9.5 μmol·L -1 MnSO4, 0.15 μmol·L -1 ZnSO4, 0.15 μmol·L -1 CuSO4, 0.52 μmol·L -1 (NH4)6Mo 24 , 18 μmol·L -1 H3BO3 and 0.2 mmol·L -1 NH4 + or 6 mmol·L -1 NH4 + ; pH of nutrient solution = 6.0; The whitened Andrographis paniculata seed buds are placed in a seedling tray and grown for 10 days, and then transplanted into a seedling hole tray and grown until they have a pair of true leaves and one heart, thereby obtaining Andrographis paniculata seedlings; The Andrographis paniculata seedlings were transferred to nutrient solution for hydroponics for at least 30 days, and the nutrient solution was replaced every 7 days.

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