Application of isovaleraldehyde and / or cis-2-pentenol in luring diaphorina citri

Abstract

The invention discloses application of isovaleraldehyde and / or cis-2-pentenol in luring diaphorina citri. The isovaleraldehyde and the cis-2-pentenol have luring effects on the diaphorina citri when the two compounds are independently used and jointly used. Research results show that compared with liquid paraffin, the attraction efficiency of 0.1-10 [mu] L / mL of isovaleraldehyde, 1-10 [mu] L / mL of cis-2-pentenol and a mixed solution of isovaleraldehyde and cis-2-pentenol with the final concentration of 4-6 [mu] L / mL on diaphorina citri are obviously different, and the isovaleraldehyde and the cis-2-pentenol are simple, convenient, efficient and easy to implement in the diaphorina citri attraction aspect. Therefore, isovaleraldehyde and / or cis-2-pentenol have / has a good application prospect in the aspect of luring diaphorina citri.

Description

Technical Field

[0001] The invention belongs to the technical field of plant disease and insect pest control, and specifically relates to the application of isovaleraldehyde and / or cis-2-pentenol in attracting citrus psyllids. Background Art

[0002] Asian citrus psyllid (Diaphorina citri Kuwayama, ACP), belonging to the order Hemiptera, family Psyllidae, is an important vector of citrus Huanglongbing. The feeding of citrus psyllid will cause the plant's tender shoots to shrink and dry up, and the new leaves to twist and deform, hindering the growth and development of the host plant, causing serious losses to the citrus industry. The wide distribution of citrus psyllid not only affects the health of the plant, but also accelerates the spread of Huanglongbing, which is not conducive to the cultivation and management of citrus. At present, the main methods used are plant quarantine, physical control and chemical control. Among them, chemical control technology is currently the most widely used type of control method. However, due to the long-term use of pesticides, it has caused serious harm to the environment. The secondary metabolites of plants have a regulatory effect on the behavior of insects. Therefore, research and development of green and environmentally friendly control technologies are very important for the prevention and control of citrus psyllid.

[0003] At present, patent CN 110122487 A discloses the use of alcohol in attracting citrus psyllids and a citrus psyllid attractant, wherein the alcohol is n-octanol or trans-1,2-cyclohexanediol. Therefore, providing more volatile compounds that can attract citrus psyllids can increase the selectivity of citrus psyllid control. Summary of the invention

[0004] The purpose of the present invention is to overcome the above defects and shortcomings in the prior art and provide the use of isovaleraldehyde and / or cis-2-pentenol in attracting citrus psyllids.

[0005] The second object of the present invention is to provide the use of isovaleraldehyde and / or cis-2-pentenol as citrus psyllid attractants.

[0006] The third object of the present invention is to provide the use of isovaleraldehyde and / or cis-2-pentenol in the preparation of citrus psyllid attractant products.

[0007] The fourth object of the present invention is to provide the use of isovaleraldehyde and / or cis-2-pentenol in the preparation of a preparation for preventing and controlling citrus Huanglongbing caused by citrus psyllid.

[0008] A fifth object of the present invention is to provide a method for controlling citrus psyllids.

[0009] A sixth object of the present invention is to provide a device for controlling citrus psyllids.

[0010] The above-mentioned object of the present invention is achieved through the following technical solutions:

[0011] In the process of testing volatile substances, the present invention screened and found that isovaleraldehyde and / or cis-2-pentenol have an attracting effect on adult citrus psyllids, and a Y-type olfactometer behavior test experiment is carried out on the two substances, one side of the Y-type tube is connected to the gas of the substance to be tested, and the other side is connected to the gas of the control, and the adult citrus psyllids are placed in the tube. The test finds that most of the citrus psyllids enter the tube arm connected to the gas of the substance to be tested, and a small amount enters the control tube arm. By comparison, it is found that isovaleraldehyde, cis-2-pentenol or a mixed solution of isovaleraldehyde and cis-2-pentenol has an attracting effect on citrus psyllids.

[0012] Therefore, the present invention provides the use of isovaleraldehyde and / or cis-2-pentenol in attracting citrus psyllids.

[0013] Furthermore, the application is the application of isovaleraldehyde and / or cis-2-pentenol in attracting citrus psyllids in agriculture. The above two volatile compounds or their combination can attract citrus psyllids and the attracting effect is significant. At a certain concentration, the attracting efficiency of citrus psyllids is above 55%, and can be used as citrus psyllid attractants in citrus production.

[0014] Therefore, the present invention also provides the use of isovaleraldehyde and / or cis-2-pentenol as citrus psyllid attractants.

[0015] The present invention also provides the use of isovaleraldehyde and / or cis-2-pentenol in preparing citrus psyllid attracting products.

[0016] Furthermore, when isovaleraldehyde is used alone, the concentration of isovaleraldehyde in the attractant is 0.1 to 10 μL / mL.

[0017] Preferably, when isovaleraldehyde is used alone, the concentration of isovaleraldehyde in the attractant is 0.1-1 μL / mL.

[0018] More preferably, when isovaleraldehyde is used alone, the concentration of isovaleraldehyde in the attractant is 0.1 μL / mL.

[0019] Furthermore, when cis-2-pentenol is used alone, the concentration of cis-2-pentenol in the attractant is 1 to 10 μL / mL.

[0020] Preferably, when cis-2-pentenol is used alone, the concentration of cis-2-pentenol in the attractant is 10 μL / mL.

[0021] Furthermore, when isovaleraldehyde and cis-2-pentenol are used in combination, the final concentrations of isovaleraldehyde and cis-2-pentenol in the attractant are both 4-6 μL / mL, that is, the attractant is a mixed solution of 0.4-0.6% cis-2-pentenol and 0.4-0.6% isovaleraldehyde.

[0022] Furthermore, the solvent of the attractant is liquid paraffin.

[0023] Furthermore, when isovaleraldehyde and cis-2-pentenol are used in combination, the volume percentages of isovaleraldehyde and cis-2-pentenol in liquid paraffin are both 0.4% to 0.6%.

[0024] Preferably, when isovaleraldehyde and cis-2-pentenol are used in combination, the volume percentages of isovaleraldehyde and cis-2-pentenol in liquid paraffin are both 0.5%.

[0025] Citrus psyllids are important vectors of citrus Huanglongbing. Feeding by citrus psyllids can cause the plant's tender shoots to shrink and dry up, and new leaves to twist and deform, hindering the growth and development of host plants and causing serious losses to the citrus industry. The wide distribution of citrus psyllids not only affects the health of the plants, but also accelerates the spread of Huanglongbing, which is not conducive to the cultivation and management of citrus. Therefore, the present invention also provides the use of isovaleraldehyde and / or cis-2-pentenol in the preparation of a preparation for preventing and treating citrus Huanglongbing caused by citrus psyllids.

[0026] The present invention also provides a method for preventing and controlling citrus psyllids, which comprises using isovaleraldehyde and / or cis-2-pentenol to lure and kill the citrus psyllids.

[0027] The present invention also provides a device for controlling citrus psyllids, which comprises a carrier and isovaleraldehyde or / and cis-2-pentenol loaded on the carrier; the carrier is an insecticidal lamp, a trapping barrel or an insect trapping station.

[0028] Furthermore, the citrus psyllid is an adult citrus psyllid.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The present invention provides the use of isovaleraldehyde and / or cis-2-pentenol in attracting citrus psyllids. The isovaleraldehyde and cis-2-pentenol involved are both plant volatile substances, belonging to plant secondary metabolites, and have an attracting effect on citrus psyllids when used alone or together. The research results in the present invention show that the attracting efficiency of 0.1-10 μL / mL of isovaleraldehyde, 1-10 μL / mL of cis-2-pentenol, and a mixed solution of isovaleraldehyde and cis-2-pentenol with a final concentration of 4-6 μL / mL respectively on citrus psyllids is significantly different from that of liquid paraffin. Both are simple, efficient and easy to implement in attracting citrus psyllids, and are easy to degrade in the environment without causing long-term environmental pollution. Isovaleraldehyde and cis-2-pentenol are cheap in market and easy to obtain, providing an effective method for further preventing and controlling citrus Huanglongbing. Therefore, both have good application prospects in attracting citrus psyllids. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 Schematic diagram of behavioral testing of citrus psyllids using a Y-shaped olfactometer.

[0032] Figure 2 This is the selective response of citrus psyllid to isovaleraldehyde. Note: ** in the figure indicates that the data are extremely significantly different (P<0.01) after t-test, and * indicates that the data are significantly different (0.01 <P<0.05)。

[0033] Figure 3 This is the selective response of citrus psyllid to cis-2-pentenol. Note: ** in the figure indicates that the data are significantly different after t-test (P<0.01).

[0034] Figure 4 This is the selective response of citrus psyllid to the mixed solution of isovaleraldehyde and cis-2-pentenol. Note: ** in the figure indicates that the data are extremely significantly different after t-test (P<0.01). DETAILED DESCRIPTION

[0035] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0036] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0037] Isovaleraldehyde: molecular formula C5H 10 O, CAS No. 590-86-3, purity ≥ 98%; cis-2-pentenol: molecular formula C5H 10 O, CAS No. 1576-95-0, purity ≥ 95%. All were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0038] Liquid paraffin was purchased from Guangzhou Dingguo Biotechnology Co., Ltd.

[0039] The Y-type olfactometer was purchased from Nanjing Shelley Biotechnology Co., Ltd.

[0040] Example 1 Testing of the Olfactory Response of Isovaleraldehyde to Citrus Psyllid Adults

[0041] The test of the olfactory response of isovaleraldehyde to citrus psyllid adults includes the following steps:

[0042] (1) Use a pipette to accurately pipette 10 μL of the volatiles required for the test into a 2 mL centrifuge tube, then add 990 μL of liquid paraffin, mix thoroughly on an oscillator to make a 10 μL / mL solution, and then dilute with liquid paraffin in a 10-fold gradient to make solutions with concentrations of 10 μL / mL, 1 μL / mL, 0.1 μL / mL, 0.01 μL / mL, and 0.001 μL / mL, and test in the laboratory.

[0043] (2) Diluted isovaleraldehyde and control reagent liquid paraffin were placed on both sides of the Y-shaped arm. The citrus psyllid adults were placed at the bottom of the central tube of the Y-shaped olfactometer and their choice of the two arms was observed. The experiment was conducted indoors using a Y-shaped olfactometer, and the test time was between 10:00 and 17:00.

[0044] (3) Use polytetrafluoroethylene tubes to connect the vacuum pump, meter, activated carbon filter, distilled water, two odor source bottles, and Y-tube on both sides of the Y-shaped olfactometer. Connect the power supply and let in air (such as Figure 1 As shown). Before the experiment, qualitative filter paper (9 cm in diameter) was placed in two odor source bottles, and 20 μL of the test solution was added to the filter paper as the treatment group, and 20 μL of liquid paraffin was added to the control group. The power was turned on and the air was connected. The air passed through the vacuum pump and the gas flow rate was controlled at 300-400 mL / min. Before each test, the power was turned on and the Y-type olfactometer was ventilated for 10 minutes. When the volatile odor of the test object filled the entire Y-type tube, the test was started. The psyllids were starved for 4 hours, and then citrus psyllids were placed at the entrance of the central tube of the Y-type tube. Each psyllid had 10 minutes to respond. If the citrus psyllid crawled to one side of the Y-type tube, crawled over 1 / 3 of the side wall, and stayed for more than 30 seconds, it was considered to have made a choice. If it did not enter any side of the Y-type tube within the specified time, it was considered to have no response. The unresponsive psyllids were not included in the statistics. Each experiment was repeated 5 times, and 15 female insects and 15 male insects were used in each repetition. The psyllids that had been tested were no longer used. After each experimental repetition, the device was disinfected with 75% ethanol, then washed with clean water and air-dried. The two odor source bottles of the olfactometer were exchanged every 15 psyllids tested to eliminate the influence of position.

[0045] The selection results of citrus psyllids to different concentrations of isovaleraldehyde are shown in Table 1.

[0046] Table 1 Selection of citrus psyllids for different concentrations of isovaleraldehyde

[0047]

[0048] Note: The data in column N in the table are the total number of insects tested (the total number of insects that made a choice); ** indicates that the difference between the treatment and the control is extremely significant after the independent sample t test (P < 0.01); * indicates that the difference between the treatment and the control is significant after the independent sample t test (0.01 < P < 0.05). Reaction rate (%) = total number of insects that made a choice / total number of insects tested × 100%; selection rate (%) = total number of insects that chose the treatment flavor source bottle / total number of insects that made a choice.

[0049] The results are as follows Figure 2 As shown, when the concentration of isovaleraldehyde was 10 μL / mL, 1 μL / mL, and 0.1 μL / mL, there were significant differences between the treatment group and the control group. Isovaleraldehyde had a significant attracting effect on citrus psyllids, and the selection rates for citrus psyllids were 56%, 55%, and 56%, respectively.

[0050] Example 2 Test of the Olfactory Response of Cis-2-Pentenol to Adult Citrus Psyllids

[0051] The test of the olfactory response of cis-2-pentenol to citrus psyllid adults comprises the following steps:

[0052] (1) According to the method of Example 1, 10 μL of cis-2-pentenol required for the test was accurately pipetted into a 2 mL centrifuge tube with a pipette, and then 990 μL of liquid paraffin was added. The mixture was thoroughly mixed on an oscillator to prepare a 10 μL / mL solution. The concentration of the solution was then decreased by 10 times and diluted with liquid paraffin to prepare solutions with concentrations of 10 μL / mL, 1 μL / mL, 0.1 μL / mL, 0.01 μL / mL, and 0.001 μL / mL, and the solution was tested in the laboratory.

[0053] (2) Diluted cis-2-pentenol and control reagent liquid paraffin were placed on both sides of the Y-shaped arm. The citrus psyllid adults were placed at the bottom of the central tube of the Y-shaped olfactometer and their choice of the two arms was observed. The experiment was conducted indoors using a Y-shaped olfactometer, and the test time was between 10:00 and 17:00.

[0054] (3) Use polytetrafluoroethylene tubes to connect the vacuum pump, meter, activated carbon filter, distilled water, two odor source bottles, and Y-tube on both sides of the Y-shaped olfactometer. Connect the power supply and let in air (such as Figure 1As shown). Before the experiment, qualitative filter paper (9 cm in diameter) was placed in two odor source bottles, and 20 μL of the test solution was added to the filter paper as the treatment group, and 20 μL of liquid paraffin was added to the control group. The power was turned on and the air was connected. The air passed through the vacuum pump and the gas flow rate was controlled at 300-400 mL / min. Before each test, the power was turned on and the Y-type olfactometer was ventilated for 10 minutes. When the volatile odor of the test object filled the entire Y-type tube, the test was started. The psyllids were starved for 4 hours, and then citrus psyllids were placed at the entrance of the central tube of the Y-type tube. Each psyllid had 10 minutes to respond. If the citrus psyllid crawled to one side of the Y-type tube, crawled over 1 / 3 of the side wall, and stayed for more than 30 seconds, it was considered to have made a choice. If it did not enter any side of the Y-type tube within the specified time, it was considered to have no response. The unresponsive psyllids were not included in the statistics. Each experiment was repeated 5 times, and 15 female insects and 15 male insects were used in each repetition. The psyllids that had been tested were no longer used. After each experimental repetition, the device was disinfected with 75% ethanol, then washed with clean water and air-dried. The two odor source bottles of the olfactometer were exchanged every 15 psyllids tested to eliminate the influence of position.

[0055] The selection results of citrus psyllids on different concentrations of cis-2-pentenol are shown in Table 2.

[0056] Table 2 Selection of citrus psyllids for different concentrations of cis-2-pentenol

[0057]

[0058] Note: The data in column N in the table are the total number of insects tested (the total number of insects that made a choice); ** indicates that the difference between the treatment and the control is extremely significant after the independent sample t test (P < 0.01); * indicates that the difference between the treatment and the control is significant after the independent sample t test (0.01 < P < 0.05). Reaction rate (%) = total number of insects that made a choice / total number of insects tested × 100%; selection rate (%) = total number of insects that chose the treatment flavor source bottle / total number of insects that made a choice.

[0059] The results are as follows Figure 3 As shown, when the concentration of cis-2-pentenol was 10 μL / mL and 1 μL / mL, there were significant differences between the treatment group and the control group. Cis-2-pentenol had a significant attractant effect on citrus psyllids, and the selection rates for citrus psyllids were 60% and 55%, respectively.

[0060] Example 3 Testing of the Olfactory Response of Isovaleraldehyde and Cis-2-Pentenol to Citrus Psyllid Adults

[0061] The test of the olfactory response of the mixed solution of isovaleraldehyde and cis-2-pentenol to the citrus psyllid adults comprises the following steps:

[0062] (1) According to the method of Example 1, the isovaleraldehyde and cis-2-pentenol required for the test were mixed with a pipette, placed in a 2 mL centrifuge tube, fully mixed on an oscillator, and configured into mixed solutions of different concentrations, and tested in the laboratory. Combination 1 is a mixed solution of 0.5% cis-2-pentenol and 0.5% isovaleraldehyde (the balance is liquid paraffin), combination 2 is a mixed solution of 0.5% cis-2-pentenol and 0.05% isovaleraldehyde (the balance is liquid paraffin), combination 3 is a mixed solution of 0.05% cis-2-pentenol and 0.5% isovaleraldehyde (the balance is liquid paraffin), and combination 4 is a mixed solution of 0.05% cis-2-pentenol and 0.05% isovaleraldehyde (the balance is liquid paraffin).

[0063] (2) A diluted solution of isovaleraldehyde and cis-2-pentenol and a control reagent, liquid paraffin, were placed on both sides of the Y-shaped arm. Adult citrus psyllids were placed at the bottom of the central tube of the Y-shaped olfactometer to observe their choice of the two arms. The experiment was conducted indoors using a Y-shaped olfactometer, and the test time was between 10:00 and 17:00.

[0064] (3) Use polytetrafluoroethylene tubes to connect the vacuum pump, meter, activated carbon filter, distilled water, two odor source bottles, and Y-tube on both sides of the Y-shaped olfactometer. Connect the power supply and let in air (such as Figure 1 As shown). Before the experiment, qualitative filter paper (9 cm in diameter) was placed in two odor source bottles, and 20 μL of the test solution was added to the filter paper as the treatment group, and 20 μL of liquid paraffin was added to the control group. The power was turned on and the air was connected. The air passed through the vacuum pump and the gas flow rate was controlled at 300-400 mL / min. Before each test, the power was turned on and the Y-type olfactometer was ventilated for 10 minutes. When the volatile odor of the test object filled the entire Y-type tube, the test was started. The psyllids were starved for 4 hours, and then citrus psyllids were placed at the entrance of the central tube of the Y-type tube. Each psyllid had 10 minutes to respond. If the citrus psyllid crawled to one side of the Y-type tube, crawled over 1 / 3 of the side wall, and stayed for more than 30 seconds, it was considered to have made a choice. If it did not enter any side of the Y-type tube within the specified time, it was considered to have no response. The unresponsive psyllids were not included in the statistics. Each experiment was repeated 5 times, and 15 female insects and 15 male insects were used in each repetition. The psyllids that had been tested were no longer used. After each experimental repetition, the device was disinfected with 75% ethanol, then washed with clean water and air-dried. The two odor source bottles of the olfactometer were exchanged every 15 psyllids tested to eliminate the influence of position.

[0065] The selection results of citrus psyllids on mixed solutions of isovaleraldehyde and cis-2-pentenol at different concentrations are shown in Table 3.

[0066] Table 3 Selection of citrus psyllids for different mixed solutions

[0067]

[0068] Note: The data in column N in the table are the total number of insects tested (the total number of insects that made a choice); ** indicates that the difference between the treatment and the control is extremely significant after the independent sample t test (P < 0.01). Reaction rate (%) = total number of insects that made a choice / total number of insects tested × 100%; selection rate (%) = total number of insects that chose the treatment odor source bottle / total number of insects that made a choice.

[0069] The results are as follows Figure 4 As shown, when the solution is a mixture of 0.5% isovaleraldehyde and 0.5% cis-2-pentenol (the balance is liquid paraffin), there is a significant difference between the treatment group and the control group, the mixed solution has a significant attraction to citrus psyllid, and the selection rate of citrus psyllid is 63%; at the same time, when the solution of the treatment group is a mixture of 0.4% isovaleraldehyde and 0.4% cis-2-pentenol (the balance is liquid paraffin), 0.4% isovaleraldehyde and 0.6% cis-2-pentenol (the balance is liquid paraffin), 0.6% isovaleraldehyde and 0.4% cis-2-pentenol (the balance is liquid paraffin) or 0.6% isovaleraldehyde and 0.6% cis-2-pentenol (the balance is liquid paraffin), there is a significant difference between the treatment group and the control group, the mixed solution has a significant attraction to citrus psyllid, and the selection rate of citrus psyllid is greater than 55%.

[0070] The above description is only a preferred embodiment of the present invention, and not a limitation on the protection scope of the present invention. For ordinary technicians in this field, other forms of changes can be made based on the above description and ideas. Without departing from the principle of the present invention, several improvements and modifications can be made. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. The use of isovaleraldehyde and / or cis-2-pentenol in attracting citrus psyllids.

2. Use of isovaleraldehyde and / or cis-2-pentenol as attractants for citrus psyllids.

3. Application of isovaleraldehyde and / or cis-2-pentenol in the preparation of citrus psyllid attractant products.

4. The use according to any one of claims 1 to 3, characterized in that: The concentration of the isovaleraldehyde is 0.1-10 μL / mL.

5. The use according to any one of claims 1 to 3, characterized in that: The concentration of the cis-2-pentenol is 1 to 10 μL / mL.

6. The use according to any one of claims 1 to 3, characterized in that: The final concentrations of isovaleraldehyde and cis-2-pentenol are 4-6 μL / mL, respectively.

7. The use according to any one of claims 1 to 3, characterized in that: The citrus psyllid is an adult citrus psyllid.

8. Use of isovaleraldehyde and / or cis-2-pentenol in the preparation of a preparation for preventing and controlling citrus Huanglongbing caused by citrus psyllid.

9. A method for controlling citrus psyllids, characterized in that: Isovaleraldehyde and / or cis-2-pentenol are used to lure and kill citrus psyllids.

10. A device for controlling citrus psyllids, characterized in that: The invention comprises a carrier and isovaleraldehyde or / and cis-2-pentenol loaded on the carrier; the carrier is an insecticidal lamp, a trapping barrel or an insect trapping station.

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