White gourd extract and preparation method thereof
By optimizing the enzymatic ultrasonic extraction process, the seasonal limitation of winter melon and the unused skin components are solved, the effective ingredient content and utilization rate of winter melon extract are improved, and efficient winter melon extraction and processing are achieved.
Patent Information
- Application Number
- CN202411592104.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-08
- Publication Date
- 2025-01-03
Smart Images
Figure CN119235012A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of natural plant extracts, and particularly relates to a wax gourd extract and a preparation method thereof. Background Art
[0002] Winter melon is a plant of the genus Winter melon in the Cucurbitaceae family that is widely distributed in Asia. It has a fresh and elegant flavor, is rich in vitamins, potassium, niacin, dietary fiber and other ingredients, and has a very low fat content (about 0.2%). In the daily diet of residents, there are a variety of dishes developed with winter melon as an ingredient, such as fried winter melon, winter melon and pork ribs soup, and dried shrimp and winter melon, which are popular among consumers as common vegetables. In addition, winter melon has many recognized health benefits, such as clearing heat, protecting the kidneys and promoting diuresis, and reducing fat and weight. For this reason, winter melon has also been developed into foods such as winter melon tea, winter melon candy and dried winter melon. As for the function of reducing fat and weight, it mainly comes from the presence of succinic acid in the flesh of winter melon, and the richness of cucurbitacin in the flesh of winter melon. Succinic acid can effectively control the conversion of sugars into fat in the body and prevent fat accumulation in the body; while cucurbitacin can promote metabolism and inhibit the conversion of sugars into fat.
[0003] However, as a seasonal vegetable in summer and autumn, the time of picking and eating wax gourd limits its processing purposes in a wider range of fields. In addition, the current utilization of wax gourd is mainly concentrated on wax gourd pulp, wax gourd pulp and seeds, and wax gourd peel is often discarded due to its darker color and hard texture. However, wax gourd peel contains a variety of nutrients, such as vitamin C and B vitamins, and also contains bioactive substances such as polysaccharides and flavonoids, with effects such as detumescence, diuresis and antioxidant, which is undoubtedly a waste of resources. Chinese patent CN103316068A discloses a method for preparing a wax gourd peel water extract, which processes wax gourd peel to prepare an extract by ultrasonic extraction and water extraction and alcohol precipitation, but the method only uses ultrasonic extraction and is not combined with enzymatic treatment, which will cause the release rate of the effective ingredients of the wax gourd peel to be low.
[0004] Therefore, in order to resolve the seasonal restrictions on the application of wax gourd processing industry, improve the overall utilization rate of wax gourd, and promote the release of effective active ingredients in the wax gourd extraction process, the present invention is committed to providing a parameter-optimized enzymatic hydrolysis-ultrasonic extraction process technology to prepare wax gourd extract. The wax gourd extract obtained by the present invention retains the unique sweet fragrance of wax gourd, greatly retains the effective active ingredients in the wax gourd, and has antioxidant activity, and can be directly applied to many fields of food processing. The present invention has practical significance for promoting the processing and application of wax gourd extract, and has a broad market application space. Summary of the invention
[0005] The purpose of the present invention is to provide a wax gourd extract and a preparation method thereof. In view of the current processing status of wax gourd extract and to solve the above problems, the present invention aims to fully extract wax gourd peel, pulp and pulp through enzymatic hydrolysis and ultrasonic extraction technology after optimizing parameters, retain the effective active ingredients in the wax gourd, and improve the utilization rate of the wax gourd.
[0006] In order to achieve the above object, the present invention provides the following technical solutions: A method for preparing wax gourd extract comprises the following steps: S1. Cleaning: Select fresh and disease-free wax gourds, clean them, peel them and remove the seeds to obtain wax gourd skin, wax gourd pulp and wax gourd flesh; S2, slurry preparation: adding purified water to the wax gourd peel obtained in step S1, and using a plant grinder to grind and pulp the wax gourd peel to obtain wax gourd peel pulp; The wax gourd pulp and wax gourd flesh obtained in step S1 are crushed and pulped with a plant crusher, and then boiled, and wax gourd pulp slurry is obtained after cooling, and a portion of the cooled wax gourd pulp slurry is centrifuged to obtain wax gourd pulp liquid; S3, enzymatic extraction: adding a composite enzyme preparation to the wax gourd peel pulp obtained in step S2, stirring evenly and performing composite enzymatic hydrolysis, and obtaining a wax gourd peel pulp enzymatic hydrolyzate after the enzymatic hydrolysis is completed; Add the wax gourd peel pulp enzymatic hydrolyzate to the wax gourd pulp obtained in step S2, stir and mix, and then perform secondary enzymatic hydrolysis. After the enzymatic hydrolysis is completed, the system is a wax gourd enzymatic hydrolyzate mixed liquid; S4, ultrasonic extraction: placing the wax gourd enzymatic hydrolyzed mixed solution obtained in step S3 in an ultrasonic device for ultrasonic extraction, and obtaining an ultrasonic extraction mixed solution after the extraction is completed; S5, centrifugal filtration: centrifuge and filter the ultrasonic extraction mixed solution obtained in step S4 to obtain an ultrasonic extraction solution; S6, mixing and modulating: adding the wax gourd pulp liquid obtained in step S2 to the ultrasonic extract obtained in step S5, mixing and modulating, and obtaining a wax gourd extract; S7, concentration and drying: the wax gourd extract obtained in step S6 is concentrated and freeze-dried to obtain a wax gourd extract.
[0007] Furthermore, in step S2, the ratio of wax gourd peel to pure water is 1:1.5~1:2 (m / v).
[0008] Furthermore, the cooking conditions in step S2 are: temperature of 60-80°C, time of 40-60 min; cooling to a temperature of 45-55°C after cooking.
[0009] Furthermore, in step S3, the composite enzyme preparation is cellulase (100,000 U / g) and pectinase (500,000 U / g), the ratio of the two enzymes is 1:0.5~1:2, the total addition amount of the two enzymes is 1~2% (g / g wax gourd peel), the enzymolysis temperature is 45~50°C, the pH is 4.5~5.5, and the enzymolysis time is 50~70 min.
[0010] Furthermore, in step S3, the mass ratio of wax gourd peel pulp enzymatic hydrolyzate to wax gourd pulp pulp is 1:2-1:2.5; the secondary enzymatic hydrolysis time is 60 min, the pH is 4.5-5.0, and the enzymatic hydrolysis temperature is 45-55°C.
[0011] Furthermore, the conditions for ultrasonic extraction in step S4 are as follows: ultrasonic frequency 30-40 kHz, ultrasonic power 150-300 W, ultrasonic time 60-90 min, and temperature 50-70°C.
[0012] Furthermore, in step S6, the ratio of the ultrasonic extract to the wax gourd pulp liquid is 1:1-1.5:1 (v / v).
[0013] Furthermore, the wax gourd extract obtained in step S7 is a slightly yellow solid powder, and the extraction rate is 9.13% to 9.22%.
[0014] A wax gourd extract prepared by a method for preparing a wax gourd extract, characterized in that the wax gourd extract has a succinic acid content of 14.83-15.77 mg / g, a trigonelline content of 0.23-0.25 mg / g, and a DPPH free radical scavenging ability IC50 value of 10.31-12.75 mg / mL.
[0015] The beneficial effects of the present invention are: The invention provides an efficient preparation process of wax gourd extract, which uses enzymatic hydrolysis and ultrasonic extraction technology with optimized parameters to fully extract the effective components of wax gourd peel, pulp and flesh, effectively promote the release of effective components succinic acid and trigonelline in wax gourd, and improve the extraction rate of active components in wax gourd and the utilization rate of wax gourd. The wax gourd extract prepared by the invention has a unique sweet fragrance of wax gourd, and meets the market demand for natural and healthy products.
[0016] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1 The present invention is a flowchart of a wax gourd extract and a preparation method thereof according to an embodiment of the present invention. DETAILED DESCRIPTION
[0018] The technical scheme of the present invention is further described below by specific implementation methods. Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. In addition, the embodiments should be understood as illustrative rather than limiting the scope of the present invention. The essence and scope of the present invention are limited only by the claims. For those skilled in the art, without departing from the essence and scope of the present invention, various changes or modifications to the material composition and dosage in these embodiments also belong to the protection scope of the present invention.
[0019] The reagents or instruments used in the examples without indicating the manufacturer are all regular products that can be purchased through regular channels. Example 1
[0020] A wax gourd extract and a preparation method thereof, comprising the following steps: S1. Cleaning: Select fresh and disease-free wax gourds, clean them, peel them and remove the seeds to obtain wax gourd skin, wax gourd pulp and wax gourd flesh; S2. Preparation of slurry: Add an appropriate amount of water to the wax gourd peel obtained above at a ratio of 1:1.5 (m / v), and use a plant grinder to grind and pulp to obtain wax gourd peel pulp. Use a plant grinder to grind and pulp the wax gourd pulp and the wax gourd pulp obtained above together, and then cook the pulped product at 60°C for 60 min, and cool to 45°C to obtain wax gourd pulp slurry. Take a portion of the cooled wax gourd pulp slurry and centrifuge to obtain wax gourd pulp liquid; S3, enzymatic extraction: add a composite enzyme preparation of cellulase and pectinase to the wax gourd peel pulp obtained above, the addition ratio is 2:1, the total addition amount is 1.0% (g / g wax gourd peel), stir evenly and perform composite enzymatic hydrolysis, the enzymatic hydrolysis temperature is 45°C, the pH is 5.0, the enzymatic hydrolysis time is 50 min, and the wax gourd peel pulp enzymatic hydrolysate is obtained after the enzymatic hydrolysis. Add an appropriate amount of wax gourd peel pulp enzymatic hydrolysate to the other part of the wax gourd pulp pulp after cooling, the addition amount is the mass ratio of wax gourd peel enzymatic hydrolysate: wax gourd pulp pulp is 1:2. After stirring and mixing, perform enzymatic hydrolysis again, the enzymatic hydrolysis time is 60 min, the pH is 4.5, and the enzymatic hydrolysis temperature is 50°C. After the enzymatic hydrolysis is completed, the system is a wax gourd enzymatic hydrolysate mixed solution; S4, ultrasonic extraction: Place the wax gourd enzymatic hydrolyzed mixed solution in an ultrasonic device for ultrasonic extraction, with an ultrasonic frequency of 30 kHz, a power of 300 W, a time of 60 min, and a temperature of 50°C. After the extraction, an ultrasonic extraction mixed solution is obtained; S5, centrifugal filtration: centrifuge and filter the ultrasonic extraction mixture obtained above, and the obtained filtrate is the ultrasonic extraction solution; S6, mixing and modulating: adding a certain proportion of wax gourd pulp liquid into the ultrasonic extract at a ratio of 1:1 (v / v), and mixing and modulating to obtain a wax gourd extract; S7, concentration and drying: the wax gourd extract is concentrated and freeze-dried to obtain a dry product, i.e., the wax gourd extract. Example 2
[0021] A wax gourd extract and a preparation method thereof, comprising the following steps: S1. Cleaning: Select fresh and disease-free wax gourds, clean them, peel them and remove the seeds to obtain wax gourd skin, wax gourd pulp and wax gourd flesh; S2. Preparation of slurry: Add an appropriate amount of water to the wax gourd peel obtained above in a ratio of 1:2 (m / v), and use a plant grinder to grind and pulp to obtain wax gourd peel pulp. Use a plant grinder to grind and pulp the wax gourd pulp and the wax gourd pulp obtained above together, and then cook the pulped product at 70°C for 50 min, and cool to 50°C to obtain wax gourd pulp slurry. Take a portion of the cooled wax gourd pulp slurry and centrifuge to obtain wax gourd pulp liquid; S3, enzymatic extraction: add a composite enzyme preparation of cellulase and pectinase to the wax gourd peel pulp obtained above, the addition ratio is 1:1, the total addition amount is 1.5% (g / g wax gourd peel), stir evenly and perform composite enzymatic hydrolysis, the enzymatic hydrolysis temperature is 50°C, the pH is 5.5, the enzymatic hydrolysis time is 60 min, and the wax gourd peel pulp enzymatic hydrolysate is obtained after the enzymatic hydrolysis. Add an appropriate amount of wax gourd peel pulp enzymatic hydrolysate to the other part of the wax gourd pulp pulp after cooling, the addition amount is the mass ratio of wax gourd peel enzymatic hydrolysate: wax gourd pulp pulp is 1:2.5. After stirring and mixing, perform enzymatic hydrolysis again, the enzymatic hydrolysis time is 60 min, the pH is 5.0, and the enzymatic hydrolysis temperature is 45°C. After the enzymatic hydrolysis is completed, the system is a wax gourd enzymatic hydrolysate mixed solution; S4, ultrasonic extraction: Place the wax gourd enzymatic hydrolyzed mixed solution in an ultrasonic device for ultrasonic extraction, with an ultrasonic frequency of 40kHz, a power of 150W, a time of 70min, and a temperature of 60°C. After the extraction, an ultrasonic extraction mixed solution is obtained; S5, centrifugal filtration: centrifuge and filter the ultrasonic extraction mixture obtained above, and the obtained filtrate is the ultrasonic extraction solution; S6, mixing and modulating: adding a certain proportion of wax gourd pulp liquid into the ultrasonic extract, the ratio is 1:1.5 (v / v), mixing and modulating, and obtaining wax gourd extract; S7, concentration and drying: the wax gourd extract is concentrated and freeze-dried to obtain a dry product, i.e., the wax gourd extract. Example 3
[0022] A wax gourd extract and a preparation method thereof, comprising the following steps: S1. Cleaning: Select fresh and disease-free wax gourds, clean them, peel them and remove the seeds to obtain wax gourd skin, wax gourd pulp and wax gourd flesh; S2. Preparation of slurry: Add an appropriate amount of water to the wax gourd peel obtained above in a ratio of 1:2 (m / v), and use a plant grinder to grind and pulp to obtain wax gourd peel pulp. Use a plant grinder to grind and pulp the wax gourd pulp and the wax gourd pulp obtained above together, and then cook the pulped product at 80°C for 40 minutes, and cool it to 55°C to obtain wax gourd pulp slurry. Take a portion of the cooled wax gourd pulp slurry and centrifuge it to obtain wax gourd pulp liquid; S3, enzymatic extraction: add cellulase and pectinase complex enzyme preparation to the wax gourd peel pulp obtained above, the addition ratio is 1:2, the total addition amount is 2.0% (g / g wax gourd peel), stir evenly and perform complex enzymatic hydrolysis, the enzymatic hydrolysis temperature is 50°C, the pH is 5.0, the enzymatic hydrolysis time is 70 min, and the wax gourd peel pulp enzymatic hydrolysate is obtained after the enzymatic hydrolysis. Add an appropriate amount of wax gourd peel pulp enzymatic hydrolysate to the other part of the wax gourd pulp pulp after cooling, the addition amount is the mass ratio of wax gourd peel pulp enzymatic hydrolysate: wax gourd pulp pulp is 1:2.5. After stirring and mixing, perform enzymatic hydrolysis again, the enzymatic hydrolysis time is 60 min, the pH is 4.5, and the enzymatic hydrolysis temperature is 50°C. After the enzymatic hydrolysis is completed, the system is a wax gourd enzymatic hydrolysate mixed solution; S4, ultrasonic extraction: Place the wax gourd enzymatic hydrolyzed mixed solution in an ultrasonic device for ultrasonic extraction, with an ultrasonic frequency of 35kHz, a power of 250W, a time of 80min, and a temperature of 60°C. After the extraction, an ultrasonic extraction mixed solution is obtained; S5, centrifugal filtration: centrifuge and filter the ultrasonic extraction mixture obtained above, and the obtained filtrate is the ultrasonic extraction solution; S6, mixing and modulating: adding a certain proportion of wax gourd pulp liquid into the ultrasonic extract, the ratio is 1:1.2 (v / v), and mixing and modulating to obtain wax gourd extract; S7, concentration and drying: the wax gourd extract is concentrated and freeze-dried to obtain a dry product, i.e., the wax gourd extract.
[0023] Furthermore, in order to further illustrate the advantages of the technical solution of the present application and the difference from the currently disclosed technology, the present invention also provides the following comparative examples. Comparative Example
[0024] A wax gourd extract and a preparation method thereof, comprising the following steps: S1. Preparing wax gourd pulp: selecting fresh and disease-free wax gourd, cleaning it, removing the seeds, slicing the wax gourd peel, wax gourd pulp and wax gourd flesh, and then crushing and pulping them using a plant crusher to obtain wax gourd pulp; S2, enzymatic hydrolysis: add cellulase and pectinase complex enzyme preparation to wax gourd pulp, the addition ratio is 1:1, the total addition amount is 2.0% (g / g wax gourd pulp), stir evenly and perform complex enzymatic hydrolysis, the enzymatic hydrolysis temperature is 50℃, pH is 5.0, and the enzymatic hydrolysis time is 70 min. After the enzymatic hydrolysis is completed, the wax gourd enzymatic hydrolyzate is obtained; S3, ultrasonic extraction: Place the wax gourd enzymatic hydrolysate in an ultrasonic device for ultrasonic extraction, with an ultrasonic frequency of 40 kHz, a power of 250 W, a time of 70 min, and a temperature of 70°C. After the extraction, an ultrasonic extract is obtained; S4, centrifugal filtration: centrifuge and filter the ultrasonic extraction mixture obtained above, and the obtained filtrate is the wax gourd extract; S5, concentration and drying: the wax gourd extract is concentrated and freeze-dried to obtain the wax gourd extract.
[0025] The wax gourd extracts obtained in Example 1, Example 2, Example 3 and the comparative example were sampled and tested. The present invention uses high performance liquid chromatography to determine the content of tartronic acid and trigonelline in the wax gourd extract, and uses a DPPH free radical scavenging experiment to determine the antioxidant activity of the extract, thereby determining the extraction rate, effective component content and antioxidant activity of the above four wax gourd extracts, and the specific results are shown in Tables 1 and 2 below.
[0026] Table 1 Yield of wax gourd extract Group Example 1 Example 2 Example 3 Comparative Example Yield (%) 9.13±0.04** 9.14±0.12** 9.22±0.09** 8.59±0.12 Note: ** indicates that the embodiment has a very significant difference compared with the comparative example ( P <0.01).
[0027] Table 2 Determination of active ingredient content of wax gourd extract Group Malonic acid (mg / g) Trigonelline (mg / g) IC50(mg / mL) Example 1 14.83±0.05 0.25±0.01* 10.31±0.44** Example 2 15.23±0.20 0.23±0.02 12.75±0.39** Example 3 15.77±0.43 0.25±0.01* 11.93±0.35** Comparative Example 13.87±1.12 0.19±0.01 16.04±0.45 Note: The scavenging ability of DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radicals is expressed by the half scavenging concentration IC50. * indicates that the example has a significant difference compared with the comparative example ( P <0.05, ** indicates that the embodiment has a very significant difference compared with the comparative example ( P <0.01).
[0028] As shown in Table 1, the yield of wax gourd extract in the embodiments of the present invention is between 9.13% and 9.22%. Compared with the comparative example, the yield of each embodiment is significantly higher (P<0.01).
[0029] As shown in Table 2, the content of tartronic acid in the wax gourd extract of the embodiments of the present invention is 14.83-15.77 mg / g, and the content of trigonelline is 0.23-0.25 mg / g, which are higher than those in the comparative example. Among them, the content of trigonelline in the wax gourd extract of embodiments 1 and 3 is significantly different from that in the comparative example ( P<0.05). The antioxidant capacity of wax gourd extract is expressed as the half scavenging concentration IC50 of DPPH (1,1-diphenyl-2-trinitrophenylhydrazine) free radical scavenging capacity. The IC50 of wax gourd extract in the comparative example is 16.04 mg / mL, while the IC50 of wax gourd extract in the embodiment is 10.31~12.75 mg / mL, which is significantly lower than that of the comparative example ( P <0.01).
[0030] In summary, the preparation method of the present invention not only improves the yield of wax gourd extract, but also increases the content and antioxidant capacity of the effective ingredients succinic acid and trigonelline in the extract, and also promotes the overall utilization of wax gourd, which has practical significance for the processing and application of wax gourd extract.
[0031] The above content is a further detailed description of the present invention in combination with a specific preferred embodiment. It cannot be determined that the specific embodiments of the present invention are limited to this. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the present invention, which should be regarded as belonging to the present invention and the scope of patent protection determined by the submitted claims.
Claims
1. A method for preparing wax gourd extract, characterized in that: The following steps are involved: S1. Cleaning: Select fresh and disease-free wax gourds, clean them, peel them and remove the seeds to obtain wax gourd skin, wax gourd pulp and wax gourd flesh; S2, slurry preparation: adding purified water to the wax gourd peel obtained in step S1, and using a plant grinder to grind and pulp the wax gourd peel to obtain wax gourd peel pulp; The wax gourd pulp and wax gourd flesh obtained in step S1 are crushed and pulped with a plant crusher, and then boiled, and wax gourd pulp slurry is obtained after cooling, and a portion of the cooled wax gourd pulp slurry is centrifuged to obtain wax gourd pulp liquid; S3, enzymatic extraction: adding a composite enzyme preparation to the wax gourd peel pulp obtained in step S2, stirring evenly and performing composite enzymatic hydrolysis, and obtaining a wax gourd peel pulp enzymatic hydrolyzate after the enzymatic hydrolysis is completed; Add the wax gourd peel pulp enzymatic hydrolyzate to the wax gourd pulp obtained in step S2, stir and mix, and then perform secondary enzymatic hydrolysis. After the enzymatic hydrolysis is completed, the system is a wax gourd enzymatic hydrolyzate mixed liquid; S4, ultrasonic extraction: placing the wax gourd enzymatic hydrolyzed mixed solution obtained in step S3 in an ultrasonic device for ultrasonic extraction, and obtaining an ultrasonic extraction mixed solution after the extraction is completed; S5, centrifugal filtration: centrifuge and filter the ultrasonic extraction mixed solution obtained in step S4 to obtain an ultrasonic extraction solution; S6, mixing and modulating: adding the wax gourd pulp liquid obtained in step S2 to the ultrasonic extract obtained in step S5, mixing and modulating, and obtaining a wax gourd extract; S7, concentration and drying: the wax gourd extract obtained in step S6 is concentrated and freeze-dried to obtain a wax gourd extract.
2. The method for preparing the wax gourd extract according to claim 1, characterized in that: In step S2, the ratio of wax gourd peel to pure water is 1:1.5-1:2 (m / v).
3. The method for preparing the wax gourd extract according to claim 1, characterized in that: The cooking conditions in step S2 are: temperature of 60-80° C., time of 40-60 min; and cooling to a temperature of 45-55° C. after cooking.
4. The method for preparing the wax gourd extract according to claim 1, wherein In step S3, the composite enzyme preparation is cellulase and pectinase, the ratio of the two enzymes is 1:0.5-1:2, the total addition amount of the two enzymes is 1-2% (g / g wax gourd peel), the enzymolysis temperature is 45-50°C, the pH is 4.5-5.5, and the enzymolysis time is 50-70 min.
5. The method for preparing the wax gourd extract according to claim 1, characterized in that: In step S3, the mass ratio of wax gourd peel pulp enzymatic hydrolyzate to wax gourd pulp pulp is 1:2-1:2.5; the secondary enzymatic hydrolysis time is 60 min, the pH is 4.5-5.0, and the enzymatic hydrolysis temperature is 45-55°C.
6. The method for preparing the wax gourd extract according to claim 1, characterized in that: The conditions for ultrasonic extraction in step S4 are as follows: ultrasonic frequency 30-40 kHz, ultrasonic power 150-300 W, ultrasonic time 60-90 min, and temperature 50-70°C.
7. The method for preparing the wax gourd extract according to claim 1, characterized in that: In step S6, the ratio of the ultrasonic extraction liquid to the wax gourd pulp liquid is 1:1-1.5:1 (v / v).
8. The method for preparing the wax gourd extract according to claim 1, characterized in that: The wax gourd extract obtained in step S7 is a slightly yellow solid powder, and the extraction rate is 9.13% to 9.22%.
9. A wax gourd extract obtained by the method for preparing the wax gourd extract according to any one of claims 1 to 8, characterized in that: The wax gourd extract has a succinic acid content of 14.83-15.77 mg / g, a trigonelline content of 0.23-0.25 mg / g, and a DPPH free radical (1,1-diphenyl-2-trinitrophenylhydrazine) scavenging ability IC50 value of 10.31-12.75 mg / mL.