Recombinant collagen ionic liquid composition as well as preparation method and application thereof

Abstract

The invention provides a recombinant collagen ionic liquid composition as well as a preparation method and application thereof, and relates to the technical field of cosmetics. The recombinant collagen ionic liquid composition provided by the invention is prepared from the following components in parts by mass: 19.8 to 79.99 parts of lysine citric acid ionic liquid, 20 to 80 parts of glycerol and 0.01 to 0.2 part of recombinant collagen, the lysine citric acid ionic liquid is mainly composed of lysine and citric acid, and the molar ratio of the lysine to the citric acid is (4-1): (2-1). The recombinant collagen ionic liquid composition is good in solubility, high in transdermal efficiency, small in irritation, short in preparation period, simple in process and capable of being continuously produced.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a recombinant collagen ionic liquid composition, a preparation method and application thereof. Background Art

[0002] Hair loss is the phenomenon of hair falling from the scalp. Physiological hair loss is the normal loss of hair caused by human metabolism; pathological hair loss refers to abnormal or excessive hair loss caused by multiple factors such as genetics, hormonal changes, medical conditions, and malnutrition, resulting in sparse hair or even baldness. Alopecia is not only related to personal self-image, but may also have a profound impact on psychological and social aspects. Drug treatment is a common way to improve and treat hair loss due to its advantages such as convenience, ease of use, and sustained effect. Among them, drugs such as minoxidil and finasteride have been proven to have an effective effect on improving hair loss, but there are problems such as long use cycle, adverse reactions (such as rash, headache, hirsutism, lower limb edema, increased blood pressure), and restrictions on use (such as finasteride is not suitable for women). Therefore, it is necessary to develop functional ingredients with high safety and few side effects to promote hair growth to make up for the shortcomings of existing methods.

[0003] Studies have shown that hair loss is mainly caused by the aging of hair follicle stem cells. Type 17 collagen (ⅩⅦ collagen) has high biocompatibility and good efficacy. It is a key molecule to prevent the aging of hair follicle stem cells (Science, 2016, 351, 6273.), which can maintain the dry characteristics of hair follicles and promote hair regeneration. Among them, recombinant collagen can achieve large-scale, high-purity production and effectively avoid pathogen risks. It has broad application prospects in the fields of cosmetics, biology and biomedicine. However, recombinant collagen has a large relative molecular mass and complex structure. It is difficult to pass through multiple biological barriers in the body and is easily metabolized and decomposed through gastric acid, enzymes and enterohepatic circulation. When used to promote hair growth, more attention should be paid to the effective delivery of type 17 collagen in the target skin area to maximize the effect. Microneedles are a common method to improve the transdermal delivery efficiency of macromolecular active ingredients such as proteins and peptides, but they are highly destructive to the skin and may cause local tissue damage and pain. There is also a risk of cross-infection and allergic reactions. Ionic liquids have the advantages of strong solubility and good percutaneous absorption. After being combined with active ingredients, they can be directly applied to the skin surface to deliver active ingredients to increase targeting and reduce adverse reactions, providing a new idea for efficient delivery of collagen and promotion of hair growth. However, current ionic liquid formulations may have strong irritation and sensitization, and poor skin applicability. Therefore, there is an urgent need for a system with high percutaneous drug delivery rate and low skin irritation, which can reduce users' allergic reactions and discomfort while ensuring efficient percutaneous delivery of collagen.

[0004] Based on this, it is of great significance to provide a method for preparing a recombinant collagen ionic liquid composition with good protein solubility, high skin penetration efficiency, low skin irritation, short preparation cycle, simple process, and continuous production. Summary of the invention

[0005] The first object of the present invention is to provide a recombinant collagen ionic liquid composition to solve the above technical problems.

[0006] The second object of the present invention is to provide a method for preparing the above-mentioned recombinant collagen ionic liquid composition.

[0007] The third object of the present invention is to provide the use of the above-mentioned recombinant collagen ionic liquid composition in promoting hair follicle repair and hair growth.

[0008] In order to achieve the above objectives, the following technical solutions are adopted: In a first aspect, the present invention provides a recombinant collagen ionic liquid composition, comprising, by weight, 19.8-79.99 parts of lysine citrate ionic liquid, 20-80 parts of glycerol, and 0.01-0.2 parts of recombinant collagen; The lysine citrate ionic liquid is mainly composed of lysine and citric acid, and the molar ratio of lysine to citric acid is 4-1:2-1.

[0009] As a further technical solution, the invention comprises, by weight, 29.85-49.95 parts of lysine citrate ionic liquid, 50-70 parts of glycerol and 0.05-0.15 parts of recombinant collagen.

[0010] As a further technical solution, the preparation method of the lysine citrate ion liquid comprises: mixing lysine, citric acid and water for reaction, and removing water after the reaction to prepare the lysine citrate ion liquid.

[0011] As a further technical solution, the reaction temperature is 25-100°C; The reaction time is 12-24h.

[0012] As a further technical solution, the method of removing moisture includes reduced pressure distillation.

[0013] As a further technical solution, the recombinant collagen includes type XVII recombinant collagen.

[0014] As a further technical solution, the mixture further comprises 0-30 parts of water by weight.

[0015] In a second aspect, the present invention provides a method for preparing the above-mentioned recombinant collagen ionic liquid composition, comprising: mixing the various components in the formula amount to prepare the recombinant collagen ionic liquid composition.

[0016] As a further technical solution, the components other than the recombinant collagen are first mixed and then the recombinant collagen is added and mixed.

[0017] In a third aspect, the present invention provides the use of the above-mentioned recombinant collagen ionic liquid composition in promoting hair follicle repair and hair growth.

[0018] Compared with the prior art, the present invention has the following beneficial effects: The recombinant collagen ionic liquid composition provided by the present invention is mainly composed of lysine citrate ionic liquid, glycerol and recombinant collagen in a specific ratio, wherein the lysine citrate ionic liquid is a pure natural ingredient product, has good biocompatibility and safety, has the advantages of good skin permeability, no irritation, convenient administration, etc. The composition can effectively reduce the user's allergic and irritation reactions; contains active ingredients that promote hair growth, is easy to use, can be directly applied to the scalp area for administration, has strong targeting and high hair growth efficiency.

[0019] The method for preparing the recombinant collagen ionic liquid composition provided by the present invention has the advantages of short preparation cycle, simple process, continuous production and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1 This is the H-NMR spectrum of lysine citrate ionic liquid; Figure 2 This is the H-NMR spectrum of arginine octanoate ionic liquid; Figure 3 This is the H-NMR spectrum of proline citrate ionic liquid; Figure 4 It is a lysine citrate ionic liquid; Figure 5 It is a recombinant collagen ionic liquid composition; Figure 6 The results of the mouse hair growth experiment are as follows; Figure 7 It is a control before and after the subjects in Test Example 3 used the recombinant collagen ionic liquid composition; Figure 8 This is a control before and after test of the subjects in Test Example 4 using the recombinant collagen ionic liquid composition. DETAILED DESCRIPTION

[0022] The embodiments of the present invention will be described in detail below in conjunction with the embodiments and examples, but it will be appreciated by those skilled in the art that the following embodiments and examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work are within the scope of protection of the present invention. If specific conditions are not specified, proceed according to normal conditions or conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0023] In a first aspect, the present invention provides a recombinant collagen ionic liquid composition, which is mainly composed of lysine citrate ionic liquid, glycerol and recombinant collagen, and the mass fraction of lysine citrate ionic liquid can be, for example, but not limited to, 19.8 parts, 40 parts, 60 parts or 79.99 parts; The recombinant collagen ionic liquid composition preferably uses glycerol as a solubilizer to increase the dissolution rate of collagen, optimize the color of the composition and reduce the viscosity of the composition. The mass fraction of glycerol can be, for example, but not limited to, 20 parts, 40 parts, 60 parts or 80 parts; The mass fraction of the recombinant collagen may be, for example, but not limited to, 0.01, 0.05, 0.1, 0.15 or 0.2; The lysine citrate ionic liquid is mainly composed of lysine and citric acid, and the molar ratio of lysine to citric acid can be, for example, but not limited to, 4:1, 3:2, 2:3 or 1:2.

[0024] The recombinant collagen ionic liquid composition provided by the present invention can effectively reduce the user's allergic and irritant reactions, promote the scalp absorption of recombinant collagen, and has a good effect of promoting hair follicle growth and hair growth. In some optional embodiments, it includes 29.85-49.95 parts of lysine citrate ionic liquid, 50-70 parts of glycerol and 0.05-0.15 parts of recombinant collagen by weight.

[0025] In some optional embodiments, the method for preparing the lysine citrate ion liquid comprises: mixing lysine, citric acid and water for reaction, and removing water after the reaction to prepare the lysine citrate ion liquid.

[0026] The preparation method of the lysine citrate ion liquid is simple, convenient and low in cost.

[0027] In some optional embodiments, the reaction temperature may be, for example, but not limited to, 25°C, 50°C, 75°C or 100°C; The reaction time may be, for example, but not limited to, 12 h, 18 h or 24 h.

[0028] In some optional embodiments, the method of removing moisture includes but is not limited to vacuum distillation, or other methods of removing moisture known to those skilled in the art.

[0029] In some optional embodiments, the recombinant collagen includes type XVII recombinant collagen.

[0030] In some optional embodiments, 0-30 parts of water are further included by weight, for example, it can be, but is not limited to, 0 part, 5 parts, 10 parts, 20 parts, 25 parts or 30 parts.

[0031] In a second aspect, the present invention provides a method for preparing the above-mentioned recombinant collagen ionic liquid composition, comprising: mixing the various components in the formula amount to prepare the recombinant collagen ionic liquid composition.

[0032] The preparation method has the advantages of short cycle, simple process, continuous production and low cost.

[0033] In order to ensure the uniformity of raw material mixing and the solubility and stability of collagen in the composition, in some optional embodiments, the components other than the recombinant collagen are mixed first and then the recombinant collagen is added and mixed.

[0034] In a third aspect, the present invention provides the use of the above-mentioned recombinant collagen ionic liquid composition in promoting hair follicle repair and hair growth.

[0035] The recombinant collagen ionic liquid composition provided by the present invention can effectively reduce the user's allergic and irritation reactions, promote the scalp absorption of the recombinant collagen, and has a good effect of promoting the growth of hair follicles and hair.

[0036] The present invention is further described below by means of specific examples and comparative examples. However, it should be understood that these examples are only used for more detailed description and should not be construed as limiting the present invention in any form.

[0037] Example 1 This embodiment provides an amino acid ionic liquid, which has an appearance as Figure 4 As shown, it is mainly prepared by the following steps: Under nitrogen atmosphere, 0.4 mol of lysine was added into a round-bottom flask, and then 50 mL of deionized water was added and stirred thoroughly at 800 rpm; Dissolve 0.1 mol of citric acid in 50 mL of deionized water, add dropwise to the reaction vessel and mix well, and react at 80°C with magnetic stirring for 12 hours; After the reaction was completed, most of the water was removed by rotary evaporation at 70° C., and then the mixture was dried in a vacuum oven at 60° C. for 48 hours to remove the remaining water, thereby obtaining a lysine citrate ionic liquid (4:1).

[0038] Example 2 This embodiment provides an amino acid ionic liquid, which is mainly prepared by the following steps: Under nitrogen atmosphere, add 0.3 mol of lysine into a round-bottom flask, then add 50 mL of deionized water and stir well to mix at a speed of 800 rpm; Dissolve 0.1 mol of citric acid in 50 mL of deionized water, then add dropwise into the reaction vessel and mix well. React at 80°C with magnetic stirring for 12 hours. After the reaction was completed, most of the water was removed by rotary evaporation at 70° C., and then the residual water was removed by drying in a vacuum oven at 60° C. for 48 hours to obtain lysine citrate ionic liquid (3:1).

[0039] Example 3 This embodiment provides an amino acid ionic liquid, which is mainly prepared by the following steps: Under nitrogen atmosphere, 0.2 mol of lysine was added into a round-bottom flask, and then 50 mL of deionized water was added and stirred thoroughly at 800 rpm; Dissolve 0.1 mol of citric acid in 50 mL of deionized water, then add dropwise into the reaction vessel and mix well. React at 80°C with magnetic stirring for 12 hours. After the reaction was completed, most of the water was removed by rotary evaporation at 70° C., and then the residual water was removed by drying in a vacuum oven at 60° C. for 48 hours to obtain lysine citrate ionic liquid (2:1).

[0040] Example 4 This embodiment provides an amino acid ionic liquid, which is mainly prepared by the following steps: Under nitrogen atmosphere, 0.1 mol of lysine was added into a round-bottom flask, and then 50 mL of deionized water was added and stirred thoroughly at 800 rpm; Dissolve 0.1 mol of citric acid in 50 mL of deionized water, then add dropwise into the reaction vessel and mix well. React at 80°C with magnetic stirring for 12 hours. After the reaction was completed, most of the water was removed by rotary evaporation at 70° C., and then the residual water was removed by drying in a vacuum oven at 60° C. for 48 hours to obtain lysine citrate ionic liquid (1:1).

[0041] Example 5 This embodiment provides an amino acid ionic liquid, which is mainly prepared by the following steps: Under nitrogen atmosphere, 0.1 mol of lysine was added into a round-bottom flask, and then 50 mL of deionized water was added and stirred thoroughly at 800 rpm; Dissolve 0.15 mol of citric acid in 50 mL of deionized water, then add dropwise into the reaction vessel and mix well. React with magnetic stirring at 25°C for 24 hours. After the reaction was completed, most of the water was removed by rotary evaporation at 70°C, and then the residual water was removed by drying in a vacuum oven at 60°C for 48 hours to obtain lysine citrate ionic liquid (1:1.5).

[0042] Example 6 This embodiment provides an amino acid ionic liquid, which is mainly prepared by the following steps: Under nitrogen atmosphere, 0.1 mol of lysine was added into a round-bottom flask, and then 50 mL of deionized water was added and stirred thoroughly at 800 rpm; Dissolve 0.2 mol of citric acid in 50 mL of deionized water, then add dropwise into the reaction vessel and mix well. React at 100°C with magnetic stirring for 18 hours. After the reaction was completed, most of the water was removed by rotary evaporation at 70° C., and then the residual water was removed by drying in a vacuum oven at 60° C. for 48 hours to obtain lysine citrate ionic liquid (1:2).

[0043] The amino acid ionic liquids prepared above were experimentally verified, and the results showed that Examples 1-6 all successfully prepared ionic liquids, and the experimental results of Example 3 were as follows: H NMR spectrum Figure 1 As shown, the lysine citrate ionic liquid 1 The H NMR spectrum showed signals corresponding to methylene groups at 1.95-1.43ppm (m, -CH2), 3.029 (m, -CH2) and 3.756 (m, -CH2), and a signal corresponding to amino groups was detected at 7.46ppm (s, -NH2), representing fragments of L-lysine. The signals corresponding to methylene groups at 2.757 (q, -CH2) and 2.662 (q, -CH2) were observed to be citric acid-related fragments. The chemical shift change of 8.51 (s, -COOH) of the carboxyl group of citric acid confirmed the successful synthesis of [L-Lys][CA]. These results indicate that the [L-Lys][CA] ionic liquid was successfully prepared.

[0044] Comparative Example 1 An arginine octanoate ionic liquid (H NMR spectrum as Figure 2 ), which is different from Example 2 in that lysine is replaced by arginine, and citric acid is replaced by caprylic acid.

[0045] Comparative Example 2 A proline citrate ionic liquid (H NMR spectrum as Figure 3 ), which is different from Example 2 in that lysine is replaced by proline.

[0046] Comparative Example 3 An amino acid ionic liquid, which is different from Example 2 in that citric acid is replaced by butyric acid.

[0047] Example 7 A recombinant collagen ionic liquid composition is mainly prepared by the following steps: Water, glycerol and the amino acid ionic liquid prepared in Example 1 were uniformly mixed to obtain a mixed solution, and then type XVII recombinant collagen was dissolved in the mixed solution, and stirred at 30° C. for 10 hours to obtain a recombinant collagen ionic liquid composition.

[0048] Among them, the mass ratio of water, glycerol, amino acid ionic liquid and type XVII recombinant collagen is 10:70:20:0.1.

[0049] Example 8 A recombinant collagen ionic liquid composition is mainly prepared by the following steps: Water, glycerol and the amino acid ionic liquid prepared in Example 2 were uniformly mixed to obtain a mixed solution, and then type XVII recombinant collagen was dissolved in the mixed solution, and stirred at 30° C. for 10 hours to obtain a recombinant collagen ionic liquid composition.

[0050] Among them, the mass ratio of water, glycerol, amino acid ionic liquid and type XVII recombinant collagen is 5:60:35:0.1.

[0051] Example 9 A recombinant collagen ionic liquid composition is mainly prepared by the following steps: Water, glycerol and the amino acid ionic liquid prepared in Example 3 were uniformly mixed to obtain a mixed solution, and then type XVII recombinant collagen was dissolved in the mixed solution, and stirred at 30° C. for 10 hours to obtain a recombinant collagen ionic liquid composition.

[0052] Among them, the mass ratio of water, glycerol, amino acid ionic liquid and type XVII recombinant collagen is 30:50:19.8:0.2.

[0053] Example 10 A recombinant collagen ionic liquid composition is mainly prepared by the following steps: Glycerol and the amino acid ionic liquid prepared in Example 2 were uniformly mixed to obtain a mixed solution, and then type XVII recombinant collagen was dissolved in the mixed solution, and stirred at 30° C. for 10 hours to obtain a recombinant collagen ionic liquid composition.

[0054] Among them, the mass ratio of glycerol, amino acid ionic liquid and type XVII recombinant collagen is 20:79.99:0.01.

[0055] Embodiment 11 A recombinant collagen ionic liquid composition is mainly prepared by the following steps: Glycerol and the amino acid ionic liquid prepared in Example 3 were uniformly mixed to obtain a mixed solution, and then type XVII recombinant collagen was dissolved in the mixed solution, and stirred at 30° C. for 10 hours to obtain a recombinant collagen ionic liquid composition.

[0056] Among them, the mass ratio of glycerol, amino acid ionic liquid and type XVII recombinant collagen is 80:19.9:0.1.

[0057] Comparative Example 4 The difference from Example 8 is that the amino acid ionic liquid of Comparative Example 1 is used.

[0058] Comparative Example 5 The difference from Example 8 is that the amino acid ionic liquid of Comparative Example 2 is used.

[0059] Comparative Example 6 The difference from Example 8 is that the amino acid ionic liquid of Comparative Example 3 is used.

[0060] Comparative Example 7 The difference from Example 8 is that the amino acid ionic liquid used is different. The preparation method of the amino acid ionic liquid is as follows: Under nitrogen atmosphere, 0.1 mol of lysine was added into a round-bottom flask, and then 50 mL of deionized water was added and stirred thoroughly at 800 rpm; Dissolve 0.4 mol of citric acid in 50 mL of deionized water, then add dropwise into the reaction vessel and mix well. React at 100°C with magnetic stirring for 18 hours. After the reaction was completed, most of the water was removed by rotary evaporation at 70° C., and then the residual water was removed by drying in a vacuum oven at 60° C. for 48 hours to obtain lysine citrate ionic liquid (1:4).

[0061] Test Example 1 The effect of the recombinant collagen ionic liquid composition prepared by the present invention on promoting hair growth in mice was evaluated. Female C57BL / 6J mice (6-8 weeks old, 18-20g) were selected, and the hair on the back was cut short with an electric clipper. An appropriate amount of depilatory cream was evenly applied to the haircut area to remove residual hair, and the mice were washed with warm water and dried. The application area was about 3cm×2cm. The skin on the back of the mouse was smooth, without damage or residual hair. Afterwards, the depilatory area and the surrounding parts were disinfected with 75% ethanol. Mice with pink skin and hair in the resting phase were selected to establish a mouse hair loss model. The next day, the mice were randomly divided into groups, with 3 mice in each group, namely a blank group (given normal saline), a collagen group (the concentration of recombinant collagen was consistent with Example 8), Example 7-11 groups, and Comparative Example 4-5 groups. The appearance of the products of Example 8, Comparative Example 4, Comparative Example 5, and Comparative Example 6 is as follows Figure 5 As shown. Each group was given medication and applied to the hair loss area on the back of mice, once a day, with a dosage of 0.5 mL, covering the hair loss area, for 14 consecutive days. The hair changes of mice were observed from 1 to 21 days.

[0062] After 7 days of administration, the backs of mice in the blank group were still pink, and the hair follicles were in the resting phase; the backs of mice in the collagen group and Example 10 group (the composition had the least recombinant collagen content) showed a small amount of light gray, indicating that collagen can promote the hair resting phase to enter the growth phase, and a small amount of hair follicles grew; the Example 7, 8, 9, 11 groups and the comparative example 4-5 groups showed different degrees of hair follicle growth, among which the Example 7, 8, 11 groups and the comparative example 4, 5 groups had no significant difference, and the Example 9 group had a larger and more uniform light gray area on the back of the mice, mainly because the composition contained a higher content of recombinant protein. Figure 6As shown, after 12 days of administration, the back skin of mice in the blank group, collagen group, and Example 10 group showed light gray to gray, and shorter hair grew in some areas, among which the collagen group and Example 10 group were better than the blank group; Example 7, 8, 9, 11 groups and Comparative Example 4, 5 groups showed better hair growth, and the mice were dark gray to black, among which the hair growth of Example 9 group was the best, and there was no significant difference in hair growth in Example 7, 8, 11 groups, but better than Comparative Example 4, 5 groups; there was no significant difference in hair in Comparative Example 4, 5 groups, but better than the blank group and collagen group. After 14 days of administration, the hair of mice in the blank group and collagen group was shorter and did not fully recover to the level before depilation. The hair of some mice in Example 10 group recovered to normal levels, and the mice in Example 7, 8, 9, 11 groups and Comparative Example 4, 5 groups all recovered to normal levels, and the hair was dark gray. After stopping the administration, the mice were observed, and it was found that some mice in the comparative example 5 group began to lose hair, and the hair on the back was sparse, while the mice in the examples 7-11 group and the comparative example 4 group did not lose hair. The above results show that ionic liquids can promote hair growth in mice, but their composition affects the hair growth effect and sustainability. The ionic liquid composition of the present invention (examples 7-11 groups) has a better effect of promoting hair growth.

[0063] Test Example 2 The hair growth promoting effect of the recombinant collagen ionic liquid composition prepared by the present invention was evaluated. 80 volunteers with hair loss problems, aged 25-55 years old, were selected and divided into 4 groups, each with 20 people in one group. The compositions of comparative example 4 and embodiments 8, 9, and 11 were used respectively (the pH of comparative examples 5 and 7 could not meet the requirements of skin care products (≥pH4.5) and could not be used for human experiments; the odor of comparative example 6 was strong, causing nausea and other reactions in the subjects, and the subjects were unwilling to accept the trial) for 14 days or more, applied once a day, evenly covering the test area, and staying for 15-30 minutes; the use results and feelings were evaluated.

[0064] Table 1 .

[0065] According to the test results in Table 1, it can be seen that the recombinant collagen ionic liquid composition examples 8, 9, and 11 provided by the present invention have obvious hair growth promotion, and the effective rate is 80% or more. Compared with comparative example 4, examples 8, 9, and 11 are more excellent in safety and use experience, and the acceptance rate of the smell of the composition by the subjects is 100%, and no adverse reactions occur during use.

[0066] Test Example 3 Male, 45 years old, used the recombinant collagen ionic liquid composition in Example 8 7 times a week, applied it to the scalp, and it was completely absorbed without pressing for 15 minutes. After continuous use for 14 days, short hair grew out and the hair density increased ( Figure 7 ).

[0067] Test Example 4 Female, 50 years old, used the recombinant collagen ionic liquid composition of Example 8 7 times a week, applied it to the scalp, and it was completely absorbed without pressing for 15 minutes. After continuous use for 60 days, the hair loss phenomenon was significantly improved and the hair density was significantly increased ( Figure 8 ).

[0068] From the above results, it can be seen that the recombinant collagen ionic liquid composition provided by the present invention has good solubility, high transdermal efficiency and low irritation, short preparation cycle, simple process, and can be continuously produced. The ionic liquid composition and its preparation method are of great significance.

[0069] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A recombinant collagen ionic liquid composition, characterized in that: Calculated by weight, it includes 19.8-79.99 parts of lysine citrate ionic liquid, 20-80 parts of glycerol and 0.01-0.2 parts of recombinant collagen; The lysine citrate ionic liquid is mainly composed of lysine and citric acid, and the molar ratio of lysine to citric acid is 4-1:2-1.

2. The recombinant collagen ionic liquid composition according to claim 1, characterized in that: Calculated by weight, the invention comprises 29.85-49.95 parts of lysine citrate ionic liquid, 50-70 parts of glycerol and 0.05-0.15 parts of recombinant collagen.

3. The recombinant collagen ionic liquid composition according to claim 1, characterized in that: The preparation method of the lysine citrate ion liquid comprises: mixing lysine, citric acid and water for reaction, and removing water after the reaction is completed to prepare the lysine citrate ion liquid.

4. The recombinant collagen ionic liquid composition according to claim 3, characterized in that: The reaction temperature is 25-100°C; The reaction time is 12-24h.

5. The recombinant collagen ionic liquid composition according to claim 3, characterized in that: The method of removing moisture includes distillation under reduced pressure.

6. The recombinant collagen ionic liquid composition according to claim 1, characterized in that: The recombinant collagen includes type XVII recombinant collagen.

7. The recombinant collagen ionic liquid composition according to claim 1, characterized in that: Calculated by weight, it also includes 0-30 parts of water.

8. The method for preparing the recombinant collagen ionic liquid composition according to any one of claims 1 to 7, characterized in that: include: After mixing the various components in the formula, a recombinant collagen ionic liquid composition is prepared.

9. The preparation method according to claim 8, characterized in that: The components other than the recombinant collagen are mixed first and then the recombinant collagen is added and mixed.

10. Use of the recombinant collagen ionic liquid composition according to any one of claims 1 to 7 in promoting hair follicle repair and hair growth.

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