Chinese herbal medicine rhubarb preparation for inhibiting tomato basal stem rot and use method of Chinese herbal medicine rhubarb preparation

Abstract

The invention discloses a Chinese herbal medicine rhubarb preparation for inhibiting tomato stem rot and a use method thereof.The Chinese herbal medicine preparation is a rhubarb preparation, and the preparation method comprises the steps that a proper amount of rhubarb is weighed and put into a glass container, water with the volume 10 times that of Chinese herbal medicine is added, and soaking is conducted for 1 hour; heating for 30 minutes, filtering and collecting liquid medicine, adding the same amount of water, and heating for 30 minutes; filtering and collecting the medicine liquid, combining the medicine liquid obtained twice, and diluting to the required concentration; the application method comprises the following steps: after tomato plants are attacked, tomato seedlings are treated in a root irrigation manner once every three days, and treatment is carried out for three times in total. As a novel natural, green and pollution-free biostimulant, the rhubarb preparation can improve the resistance of tomatoes to stem rot, does not cause the residues of heavy metals and pesticides in tomato plants, and is high in safety; the formula provided by the invention is a pure traditional Chinese medicine formula, can be effectively absorbed by organisms, is not easy to cause soil environment pollution, is safe, pollution-free, residue-free, green, healthy and environment-friendly, and can effectively improve the tomato planting benefit.

Description

Technical Field

[0001] The invention relates to the technical field of plant biostimulants, in particular to a Chinese herbal rhubarb preparation for inhibiting tomato stem base rot and a use method thereof. Background Art

[0002] Tomato is one of the most widely grown nightshade vegetables in the world. It is deeply loved by people because it is rich in nutrients such as vitamins, organic acids, and carotenoids. Stem rot caused by Fusarium solani is one of the most common soil-borne diseases in greenhouse tomato growth in northern China in recent years, causing great losses to the tomato industry.

[0003] At present, chemical control is the most commonly used means to prevent and control tomato stem rot. However, long-term use of chemical pesticides will cause a series of environmental problems. Biostimulants have excellent effects on reducing weight and increasing efficiency, improving plant resistance, etc. and are widely used in agricultural production. Biostimulants mainly include humic acid, protein hydrolyzate and other nitrogen-containing compounds, seaweed extracts, chitosan and other biopolymers, inorganic compounds, microbial agents, etc. When applied to plant leaves or rhizosphere, it can stimulate plant physiological responses, thereby promoting plant absorption and utilization of nutrients, improving the ecological environment of crop rhizosphere, and improving the ability of crops to resist biological stress. In recent years, biostimulants have the effects of fertilizer enhancement and soil improvement, which have attracted the attention and choice of more and more fertilizer companies and farmer consumers.

[0004] my country is rich in Chinese herbal medicine resources. Natural Chinese herbal medicines have the characteristics of high efficiency, antibacterial, safety and no residue that ordinary chemical agents do not have. They can solve problems such as pathogen resistance, environmental pollution and pesticide residues. With the development of green agriculture, Chinese herbal medicines are increasingly widely used in biological control. Rhubarb is a perennial herb of the Polygonaceae family. Its medicinal part is the rhizome. It is a good medicine for clearing heat and detoxifying, and has a good antibacterial effect. It is cheap and rich in resources, and is an excellent resource for the development of new biostimulants. Therefore, choosing moderately priced Chinese herbal medicines, preparing them into environmentally friendly Chinese herbal medicine extracts, and applying them in the field of crop disease resistance will open up new ways for the research and development of natural, green and pollution-free biostimulants.

[0005] Therefore, we propose a Chinese herbal rhubarb preparation for inhibiting tomato stem base rot and a method for using the same. Summary of the invention

[0006] The purpose of the present invention is to provide a Chinese herbal rhubarb preparation for inhibiting tomato stem base rot and a use method thereof, which can effectively solve the problems in the background technology.

[0007] To achieve the above object, the technical solution adopted by the present invention is: In a first aspect, the present invention provides a Chinese herbal medicine preparation for inhibiting tomato stem base rot, wherein a component of the Chinese herbal medicine preparation is a rhubarb preparation.

[0008] In a second aspect, the present invention also provides a method for preparing a Chinese herbal medicine preparation for inhibiting tomato stem rot, comprising the following steps: (1) Weigh an appropriate amount of rhubarb into a glass container, add 10 times the volume of water as the Chinese herbal medicine, and soak for 1 hour; (2) Heat in a 100°C water bath for 30 minutes, filter and collect the solution using a funnel and filter paper, add an equal amount of water to the residue, and heat in a 100°C water bath for 30 minutes; (3) Use a funnel and filter paper to filter and collect the drug solution, combine the two solutions, and dilute to the required treatment concentration, which are 4, 8, and 12 mg / mL respectively.

[0009] Preferably, the water used in step 1 and step 2 is distilled water.

[0010] In a third aspect, the present invention also provides a method for inoculating tomato stem base rot, comprising the following steps: (1) When tomatoes have three leaves and one heart, use a sterilized cotton swab to pick up Fusarium solani ( Fusarium solani ) bacterial liquid, scratch the base of the stem; (2) using a hole puncher to punch holes in the PDA culture medium infested with Fusarium solani; (3) Place the mycelium block directly over the abrasion site and wrap the infected site with sterilized cotton soaked in the Fusarium solani liquid; (4) Place a transparent cover on the tray, spray water on the cover to provide moist conditions for disease, and place in a low-light location for cultivation.

[0011] In a fourth aspect, the present invention also provides a method for using a Chinese herbal medicine preparation for inhibiting tomato stem base rot: the target crop is tomato, and the target site is the base of the stem; after the tomato plants become diseased, the tomato seedlings are treated by root irrigation, once every three days, for a total of three times.

[0012] The beneficial effects of the Chinese herbal rhubarb preparation for inhibiting tomato stem base rot and the use method thereof of the present invention are specifically as follows: 1. The present invention uses a natural Chinese herbal medicine rhubarb preparation as a biostimulant, and treats tomato plants by root irrigation, which significantly reduces the symptoms of tomato stem base rot, promotes plant growth, enhances leaf photosynthetic capacity and improves antioxidant enzyme activity. Therefore, the rhubarb preparation, as a natural, green and pollution-free new biostimulant, can not only improve the resistance of tomatoes to stem base rot, but also does not cause heavy metal and pesticide residues in tomato plants, and has higher safety; 2. The formula of the present invention is a pure Chinese medicine formula, which is a natural substance with little toxic and side effects, mild efficacy, and no drug resistance. It can not only be effectively absorbed by organisms, but also is not easy to cause soil environmental pollution. It is safe, pollution-free, residue-free, green, healthy and environmentally friendly, and can effectively improve the benefits of tomato planting. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] Figure 1 Schematic diagram of the effect of different concentrations of rhubarb preparations provided by the present invention on the aboveground phenotype of tomato seedlings after grafting; wherein, Figure A is a diagram of the overall growth phenotype of the plant; Figure B is a diagram of the lesions at the base of the plant stem; Figure C is the area of ​​the lesions on the plant; Figure D is the height of the plant; Figure E is the thickness of the plant stem; sterile water indicates that the plant has no grafted stem base rot; Figure 2 Schematic diagram of the effect of rhubarb preparations with different treatment concentrations provided by the present invention on the root size of tomato after grafting; wherein, Figure A is the root phenotype of tomato; Figure B is the total root length; C is the root surface area; D is the root volume; E is the average root diameter; F is the number of root tips; G is the fractal dimension; sterile water indicates that the plant has no grafted stem rot; Figure 3 Schematic diagram of the effect of rhubarb preparations of different treatment concentrations provided by the present invention on the biomass of tomato plants after grafting; wherein, Figure A is the fresh weight of the aboveground part of the plant; Figure B is the fresh weight of the underground part; Figure C is the dry weight of the aboveground part; Figure D is the dry weight of the underground part; Figure E is the fresh root-crown ratio; Figure F is the dry root-crown ratio; sterile water indicates that the plant has no grafted stem base rot; Figure 4 Schematic diagram of the effect of rhubarb preparations of different treatment concentrations provided by the present invention on the photosynthetic parameters of tomato leaves; wherein, Figure A is the net photosynthetic rate of plant leaves; Figure B is the intercellular carbon dioxide concentration; Figure C is the stomatal conductance; Figure D is the transpiration rate; Figure E is the actual photochemical efficiency of photosystem II; Figure F is the maximum photochemical efficiency of photosystem II; sterile water indicates that the plant has no graft base rot; Figure 5 Schematic diagram of the effect of rhubarb preparations of different treatment concentrations provided by the present invention on photosynthetic pigments of tomato leaves after grafting; wherein, Figure A is the chlorophyll a content; Figure B is the chlorophyll b content; Figure C is the total chlorophyll content; Figure D is the carotenoid content; sterile water indicates that the plant has no grafted stem base rot; Figure 6Schematic diagram of the effect of rhubarb preparations with different treatment concentrations provided by the present invention on the antioxidant enzyme activity and malondialdehyde content of tomato leaves after grafting disease; Figure A is superoxide dismutase activity; Figure B is peroxidase activity; Figure C is catalase activity; Figure D is malondialdehyde content; sterile water indicates that the plant has no grafted stem base rot. DETAILED DESCRIPTION

[0015] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example

[0016] like Figures 1 to 6 As shown, the present invention provides a Chinese herbal rhubarb preparation for inhibiting tomato stem base rot, and the component of the Chinese herbal preparation is rhubarb preparation.

[0017] The Chinese herbal medicine rhubarb preparation in this embodiment is obtained by the following steps: (1) Weigh an appropriate amount of rhubarb into a glass container, add 10 times the volume of water as the Chinese herbal medicine, and soak for 1 hour; (2) Heat in a 100°C water bath for 30 minutes, filter and collect the solution using a funnel and filter paper, add an equal amount of water to the residue, and heat in a 100°C water bath for 30 minutes; (3) Use a funnel and filter paper to filter and collect the drug solution, combine the two solutions, and dilute to the required treatment concentration, which are 4, 8, and 12 mg / mL respectively.

[0018] The tomato stem base rot inoculation method in the present embodiment is as follows: (1) When tomatoes have three leaves and one heart, use a sterilized cotton swab to soak in the fungus solution of Fusarium solani and scratch the base of the stem; (2) using a hole puncher to punch holes in the PDA culture medium infested with Fusarium solani; (3) Place the mycelium block directly over the abrasion site and wrap the infected site with sterilized cotton soaked in the Fusarium solani liquid; (4) Place a transparent cover on the tray, spray water on the cover to provide moist conditions for disease, and place in a low-light location for cultivation.

[0019] The method of using the Chinese herbal medicine preparation in this embodiment is as follows: After the tomato plants become sick, treat the tomato seedlings by root irrigation, once every three days, for a total of three treatments.

[0020] in: The targeted crop is tomato, and the targeted part is the base of the stem.

[0021] Test example: The tomato variety used for the test was "Heinz 1706".

[0022] Experimental grouping: The experimental material of this study was the tomato cultivar "Heinz 1706". The seeds were first washed with 75% anhydrous ethanol on a clean bench for 1 minute, then soaked with 25% sodium hypochlorite, shaken in a shaker at 28 ℃ for 20 minutes, washed 10 times with sterile water, and the seed coat was washed until it turned slightly white before being sown in MS medium. After culturing in the dark at 28 °C for 3 days, the tomato was transferred to the light for cultivation. After the first true leaf grew out, it was transplanted into a mixed matrix of vermiculite, perlite and soil in a ratio of 1:1:2. The mixture was watered thoroughly and kept in a light cycle of 16 h (light) and 8 h (dark), a day and night temperature cycle of 25 °C / 20 °C, and a light intensity of 150 μmol·m −2 ·s −1 and grown under conditions of 50% relative humidity; After the base of the stem became diseased, the cotton was removed and the base of the stem was irrigated with rhubarb preparations at concentrations of 0, 4, 8, and 12 mg / mL, respectively. Sterile water indicated that the plants were not infected with stem base rot pathogens and were treated with sterile water. Each tomato seedling was irrigated with 30 mL of the Chinese herbal medicine preparation, and the treatment was performed once every 3 days for a total of 3 times.

[0023] Experimental method: Completely randomized block design with 6 biological replicates.

[0024] Experimental results: Refer to the attached Figure 1 Compared with the treatment of 0 mg / mL, the lesion area under the treatment of 12 mg / mL was significantly reduced by 78%; compared with the treatment of 0 mg / mL, the plant height and stem thickness of the infected tomatoes were increased to a certain extent after the rhubarb preparation was treated, and increased with the increase of treatment concentration, reaching the maximum at 12 mg / mL, which was significantly increased by 24.72% and 32.42% respectively compared with 0 mg / mL; Refer to the attached Figure 2 Compared with the treatment with 0 mg / mL, the underground root length, root surface area, root volume, and root tip number of infected tomatoes increased after being treated with rhubarb preparation, and increased with the increase of treatment concentration, reaching the maximum at the concentration of 12 mg / mL. Refer to the attached Figure 3Compared with the treatment of 0 mg / mL, the dry and fresh weights of the aboveground and underground parts of the infected tomatoes after treatment with different concentrations of rhubarb preparations were significantly increased, and increased with the increase of treatment concentration, reaching a peak at the treatment concentration of 12 mg / mL; there was no significant difference in the dry-root-crown ratio of the plants at the statistical level under different treatment concentrations, but the uninoculated control group was significantly higher than the treatment group; the fresh-root-crown ratio was the largest at the treatment concentration of 4 mg / mL, which was significantly increased by 12.89% compared with the 0 mg / mL treatment group; Refer to the attached Figure 4 The net photosynthetic rate, stomatal conductance, transpiration rate, and Fv / Fm of infected tomato leaves changed most significantly at the treatment concentration of 12 mg / mL, which were significantly increased by 41.57%, 60%, 27%, and 4.37% compared with the 0 mg / mL treatment group, respectively; the intercellular carbon dioxide concentration decreased with the increase of treatment concentration, and was the lowest at the 12 mg / mL treatment concentration, which was significantly reduced by 12.68% compared with the untreated group after infection; Refer to the attached Figure 5 The contents of chlorophyll a, chlorophyll b and total chlorophyll reached their peak values ​​at the treatment concentration of 12 mg / mL, which were significantly increased by 37.35%, 45.81% and 37.49% compared with the control, respectively; the content of carotenoids reached the highest value at the treatment concentration of 8 mg / mL, which was significantly increased by 25.44% compared with the control; Refer to the attached Figure 6 The 8 mg / mL treatment significantly increased the antioxidant enzyme activity of tomato leaves after infection, and the 12 mg / mL treatment significantly reduced the MDA (malondialdehyde) content, enhancing the stress resistance of tomato plants after infection.

[0025] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. The application of Chinese herbal medicine preparation in inhibiting tomato stem rot, characterized in that: The component of the Chinese herbal medicine preparation is rhubarb preparation; the preparation method of the Chinese herbal medicine rhubarb preparation comprises the following steps: (1) Weigh an appropriate amount of rhubarb into a glass container, add 10 times the volume of water as the Chinese herbal medicine, and soak for 1 hour; (2) Heat in a 100°C water bath for 30 minutes, filter and collect the solution using a funnel and filter paper, add an equal amount of water to the residue, and heat in a 100°C water bath for 30 minutes; (3) Use a funnel and filter paper to filter and collect the drug solution, combine the two solutions, and dilute to the required treatment concentration, which are 4, 8, and 12 mg / mL respectively.

2. The method for preparing a Chinese herbal medicine preparation for inhibiting tomato stem rot according to claim 1, characterized in that: The water used in step (1) and step (2) is distilled water.

3. A use of the Chinese herbal rhubarb preparation as claimed in claim 1 in inhibiting tomato stem rot, characterized in that: The following steps are involved: (1) When tomatoes have three leaves and one heart, treat the tomato seedlings with stem base rot; (2) After the tomato plants become diseased, the tomato seedlings are treated with the Chinese herbal rhubarb preparation once every three days for a total of three times.

4. The use of the Chinese herbal rhubarb preparation in inhibiting tomato stem rot according to claim 3, characterized in that: In step (1), the inoculation treatment of tomato seedlings against stem base rot comprises the following steps: (1) When tomatoes have three leaves and one heart, use a sterilized cotton swab to pick up the fungus Fusarium solani ( Fusarium solani ) bacterial liquid, scratch the base of the stem; (2) using a hole puncher to punch holes in the PDA culture medium infested with Fusarium solani; (3) Place the mycelium block directly over the abrasion site and wrap the infected site with sterilized cotton soaked in the Fusarium solani liquid; (4) Place a transparent cover on the tray and spray water on the cover to provide moist conditions for the disease to occur. Place the tray in a low-light location and wait for the tomato plants to become diseased.

5. The use of the Chinese herbal rhubarb preparation in inhibiting tomato stem rot according to claim 3, characterized in that: In step (2), the part of the tomato seedlings that is treated is the base of the stem, and the treatment method is root irrigation.

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