Composite plant extract with function of relieving physical fatigue as well as preparation method and application of composite plant extract

Abstract

The invention discloses a composite plant extract and application thereof in preparation of a product for relieving physical fatigue, and the composite plant extract comprises the following raw materials in parts by weight: 25-35 parts of a polygonatum sibiricum extract, 15-25 parts of a honeysuckle flower extract, 10-15 parts of a polygonatum odoratum extract, 5-10 parts of an okra extract and 5-10 parts of a mulberry extract. The raw materials including the rhizoma polygonati extract, the honeysuckle flower extract, the radix polygonati officinalis extract, the okra extract and the mulberry fruit extract used by the composite plant extract are all medicinal and edible plants, are compounded from pure natural composite plant extracts, are wide in source, are safe and healthy, and have a synergistic effect through the raw materials; therefore, the composition has an excellent effect of relieving physical fatigue.

Description

Technical Field

[0001] The invention belongs to the technical field of functional foods, and specifically relates to a composite plant extract having the function of relieving physical fatigue, and a preparation method and application thereof. Background Art

[0002] Fatigue is a normal physiological phenomenon. For normal people, it is a self-protection mechanism of the body and a signal sent by the body. If it is ignored and allowed to develop, it will develop into chronic fatigue syndrome (CFS), which is mainly manifested as severe fatigue. Even after sufficient rest, the fatigue cannot be relieved and can last for more than 6 months, resulting in a decrease in daily activity ability, accompanied by a series of manifestations such as low fever, throat discomfort and swelling and pain, joint and muscle soreness, lymph swelling and pain, and mental and neurological symptoms.

[0004] Fatigue has become a common health problem. Physical fatigue, mental fatigue, mental fatigue and pathological fatigue can seriously affect people's work efficiency and endanger human health. The main symptoms are inattention, drowsiness, depression, reduced ability to engage in normal activities, physical exhaustion and other symptoms. What's worse, it can increase the incidence of diseases such as multiple sclerosis, cerebral hemorrhage, neurasthenia and other diseases. Long-term fatigue can reduce the body's immunity, promote pathogenic microorganisms to invade the body and cause diseases. Excessive fatigue can even endanger life.

[0005] Chinese patent application CN112841654A discloses an anti-fatigue composition and an anti-fatigue health food containing the same. The anti-fatigue composition comprises 20 to 30 parts of a mixed extract and 2 to 6 parts of taurine; the mixed extract is obtained by mixing and extracting raw materials including polygonatum, wolfberry and ginseng. The anti-fatigue health food comprises the above-mentioned anti-fatigue composition and auxiliary materials. The present invention utilizes the synergistic effect of polygonatum, wolfberry, ginseng and taurine in a specific combination and ratio, so that the anti-fatigue composition and anti-fatigue health food achieve better anti-fatigue effects than some components, and the raw materials of the anti-fatigue health food are natural and have no toxic side effects. However, the composition contains a certain amount of taurine, and some people may be allergic to taurine. Taking too much may excite the nerve center, cause activation and abnormal discharge of brain cells, lead to excessive nerve excitement, and may cause insomnia, decreased concentration, etc.

[0006] Western medicine has not yet found an effective treatment for chronic fatigue syndrome, but small doses of steroids and antidepressants are still an effective treatment method. However, these drugs can only temporarily relieve the symptoms of chronic fatigue. Once they are stopped, most of the effects will disappear, and they may also cause side effects such as emotional instability.

[0007] Therefore, developing a pure natural composite plant extract with anti-fatigue effect and no toxic side effects has extremely important practical and social significance for the field. Summary of the invention

[0008] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a composite plant extract with the function of relieving physical fatigue, and a preparation method and application thereof. The raw materials used in the composite plant extract, honeysuckle extract, polygonatum extract, polygonatum odoratum extract, okra extract, and mulberry extract, are all medicinal and edible plants. They are compounded with pure natural composite plant extracts, have a wide range of sources, are safe and healthy, and through the synergistic effect between the raw materials, the composition has an excellent effect of relieving physical fatigue.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0011] 25-35 parts of polygonatum sibiricum extract, 15-25 parts of honeysuckle extract, 10-15 parts of polygonatum odoratum extract, 5-10 parts of okra extract, and 5-10 parts of mulberry extract.

[0012] Preferably, a composite plant extract having the function of relieving physical fatigue comprises the following raw materials by weight:

[0013] 30-35 parts of polygonatum sibiricum extract, 15-20 parts of honeysuckle extract, 10-13 parts of polygonatum odoratum extract, 5-8 parts of okra extract, and 5-7 parts of mulberry extract.

[0014] In the present invention, Polygonatum sibiricum, also known as tiger ginger, sun grass, and chicken head ginseng, is the dried rhizome of a perennial herbaceous plant of the genus Polygonatum of the Liliaceae family. Its chemical components mainly include Polygonatum sibiricum polysaccharide, steroidal saponins, anthraquinone compounds, alkaloids, cardiac glycosides, vitamins and various amino acids and other compounds useful to the human body; Polygonatum sibiricum polysaccharide is an important component of the chemical composition of Polygonatum sibiricum and is also one of the main biologically active ingredients of Polygonatum sibiricum. It has the effects of relaxing the stomach and replenishing qi, replenishing the kidney and filling the essence, nourishing yin and moistening the lungs, promoting fluid and replenishing the spleen, calming the five internal organs, and replenishing the essence, and has good effects in antibacterial, detoxifying, anti-fatigue, anti-aging and other aspects.

[0015] Honeysuckle, also known as honeysuckle, contains a variety of plant active ingredients that are beneficial to the human body, such as organic acids, flavonoids, volatile oils, cyclopentane glycosides, etc. Among them, organic acid components can be separated into chlorogenic acid and isochlorogenic acid with heat-clearing, detoxifying and antibacterial effects. Flavonoid components, such as luteolin and honeysuckle glycosides, are often used as the main active substances with antiviral, anti-tumor and antioxidant activities. Volatile oil components have antibacterial, anti-inflammatory and hemostatic effects. Cyclopentane glycosides play a role in protecting the liver and gallbladder.

[0016] Polygonatum odoratum is the dried rhizome of Polygonatum odoratum of the Liliaceae family. It is a dual-purpose medicine and food substance, and its main functions are to promote the production of body fluids and quench thirst, nourish yin and moisten dryness. The chemical components of Polygonatum odoratum mainly include Polygonatum odoratum polysaccharides, steroidal saponins, flavonoids, volatile oils, etc., which have pharmacological effects such as lowering blood sugar, immunomodulation, anti-tumor, anti-oxidation, anti-fatigue, and delaying skin aging.

[0017] Okra is an annual herbaceous plant of the genus Malva, also known as okra, rich in protein, free amino acids, multiple vitamins, mineral elements such as calcium, phosphorus, iron, manganese, zinc, and mucus substances composed of pectin and polysaccharides. It has the effects of anti-fatigue, anti-tumor, liver protection, and physical enhancement. It is a traditional medicinal and edible plant with high edible and medicinal value. Okra is rich in dietary fiber, among which pectin and cellulose are the highest. Studies have shown that okra contains active ingredients such as flavonoids, polysaccharides, pectins, proteins, and soluble dietary fiber.

[0018] Mulberry is the dried fruit spike of the mulberry plant of the Moraceae family. It tastes sweet and sour, is cold in nature, and enters the heart, liver, and kidney meridians. It has the functions of nourishing the liver and kidney, nourishing blood and producing body fluid, nourishing fluid and extinguishing wind, and moistening the intestines and relieving constipation. It is mainly used to treat liver and kidney yin deficiency, dark eyes and tinnitus, insufficient body fluid, blood deficiency and constipation, internal heat and thirst, scrofula, joint discomfort, neurasthenia, etc. Mulberry is rich in amino acids, vitamins, minerals, flavonoids, sugars, alkaloids and other biologically active ingredients. Polysaccharides are an important material basis for mulberry's tonic functions such as nourishing yin and blood, and have the effects of promoting immunity, lowering blood sugar, anti-aging and anti-fatigue.

[0019] Preferably, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0020] The polygonatum powder is added to petroleum ether, refluxed at 50-60°C for 3-5h for degreasing, and then dried to obtain defatted polygonatum powder; the defatted polygonatum powder is added to ethanol aqueous solution for ultrasonic extraction, filtered after the extraction is completed to obtain an extract, and the filter residue is dried for later use; the filter residue is added to deionized water, followed by cellulase and papain for enzymolysis, after the enzymolysis is completed, the enzyme is inactivated, and then water extraction is performed, after the water extraction is completed, it is filtered to obtain a water extract; the extract and the water extract are combined, and reduced pressure rotary evaporation is performed to obtain the polygonatum extract.

[0021] Preferably, the method for preparing the honeysuckle extract comprises the following steps:

[0022] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution; then add the dihydroquercetin solution to the γ-cyclodextrin solution, stir at a constant temperature to obtain a mixed solution; add honeysuckle pollen to the ethanol-water solution, soak at room temperature, add 70-80°C hot water after the soaking, then add tea theanine and the mixed solution, extract under reduced pressure under ultrasonic conditions, filter after the extraction, and perform reduced pressure rotary evaporation on the filtrate to obtain a honeysuckle extract.

[0023] In the present invention, the dihydroquercetin solution is first compounded with γ-cyclodextrin to form an inclusion solution, and then the inclusion solution is added during the extraction process of honeysuckle. The γ-cyclodextrin in the inclusion solution can improve the solubility of chlorogenic acid, increase the extraction rate and stability of chlorogenic acid, and dihydroquercetin can promote the body's absorption of chlorogenic acid, increase the bioavailability of chlorogenic acid, and synergize with the active substances in the composite plant extraction to relieve physical fatigue. At the same time, tea theanine is added during the extraction process, and the carboxyl group in theanine combines with the aromatic ring in the chlorogenic acid, which can cooperate with the chlorogenic acid in the honeysuckle extract to exert better biological activity.

[0024] Preferably, the mass concentration of the dihydroquercetin solution is 0.5-1.5%, the mass concentration of the γ-cyclodextrin solution is 4-6%, the mass ratio of the dihydroquercetin solution and the γ-cyclodextrin solution is 20-30:40-60, the temperature of the constant temperature stirring is 38-45°C, and the time is 5-8h; the mass concentration of ethanol in the ethanol-water solution is 70-80%, the mass ratio of the honeysuckle pollen, ethanol-water solution, hot water, tea theanine, and mixed solution is 40-50:100-140:900-1200:0.5-1:60-90, the immersion time is 30-40min, the power of the ultrasound is 200-300W, the pressure of the reduced pressure extraction is 20-30kpa, and the time is 10-20min.

[0025] In the present invention, after the polygonatum is defatted, it is extracted once by an ethanol-ultrasound method to extract part of the polygonatum polysaccharide and polygonatum saponin, and then the filter residue is enzymatically hydrolyzed and then water extracted, which can hydrolyze or degrade the components of the filter residue cell wall and destroy the cell wall, thereby reducing the mass transfer resistance of the polysaccharide diffusing from the cell to the extraction medium, and improving the extraction rate of the polygonatum polysaccharide and saponin.

[0026] Preferably, the volume ratio of ethanol to water in the ethanol-water solution is 7-8:2-3, the solid-liquid ratio of the defatted polygonatum and the ethanol-water solution is 1:8-12, the power of the ultrasonic extraction is 150-200W, the temperature is 55-65°C, and the time is 20-40min; the solid-liquid ratio of the filter residue and deionized water is 1:10-15, the amount of cellulase added is 2-4% of the mass of the filter residue, the amount of papain added is 1-3% of the mass of the filter residue, the temperature of the enzymatic hydrolysis is 40-45°C, and the time is 30-50min; the temperature of the water extraction is 60-70°C, and the time is 1-2h; the pressure of the vacuum rotary evaporation is 10-20kpa.

[0027] Preferably, the method for preparing the mulberry extract comprises the following steps:

[0028] The mulberry powder is added to petroleum ether, refluxed at 50-60°C for 3-5h, and then dried to obtain defatted mulberry powder; the defatted mulberry powder is added to water, and cellulase and pectinase are added after immersion to perform enzymolysis, and the enzyme is inactivated after the enzymolysis is completed, followed by microwave treatment, and after the treatment is completed, the powder is filtered, and the filtrate is subjected to reduced pressure rotary evaporation to obtain a mulberry extract.

[0029] In the present invention, mulberry powder is first defatted and then extracted by enzyme method and microwave method. On the one hand, water-soluble substances such as polysaccharides in mulberries can be extracted, thereby improving the extraction rate of active substances. On the other hand, microwaves greatly shorten the heating time. The internal temperature of the system caused by microwave radiation rises rapidly, making the heat transfer and mass transfer directions consistent, thereby improving the solubility and stability of mulberry anthocyanins, and making the obtained mulberry extract have better biological activity.

[0030] Preferably, the solid-liquid ratio of the defatted mulberry powder to water is 1:10-15; the immersion temperature is 30-40°C, and the time is 2-3h; the amount of the cellulase added is 2-4% of the mass of the defatted mulberry powder, the amount of the pectinase added is 1-3% of the mass of the defatted mulberry powder, the temperature of the enzymolysis is 40-45°C, and the time is 30-50min; the power of the microwave treatment is 300-400W, and the time is 4-8min; the pressure of the vacuum rotary evaporation is 10-20kpa.

[0031] A method for preparing the composite plant extract having the function of relieving physical fatigue as described above comprises the following steps:

[0032] The raw materials are weighed according to the formula, and the polygonatum sibiricum extract, honeysuckle extract, polygonatum odoratum extract, okra extract and mulberry extract are evenly mixed to obtain the composite plant extract.

[0033] The invention relates to an application of the composite plant extract having the function of relieving physical fatigue in food or medicine.

[0034] Preferably, the food is a health food or a functional food.

[0035] Compared with the prior art, the present invention has the following beneficial effects:

[0036] (1) The composite plant extract provided by the present invention is prepared by compounding polygonatum extract, honeysuckle extract, polygonatum odoratum extract, okra extract and mulberry extract in a specific ratio. The chlorogenic acid in the honeysuckle extract can significantly increase the liver glycogen and muscle glycogen content of mice and improve their exercise capacity. The flavonoids in the polygonatum odoratum and mulberry extracts can synergistically relieve the fatigue of mice with chlorogenic acid. The polysaccharides in the polygonatum and okra extracts can also form a complex with the chlorogenic acid in the honeysuckle, thereby significantly improving the efficacy of the composite plant composition.

[0037] (2) The composite plant extract provided by the present invention includes a polygonatum extract, wherein the polygonatum is first defatted, and then extracted once by an ethanol-ultrasound method to extract part of the polygonatum polysaccharides and polygonatum saponins, and then the residue is enzymatically hydrolyzed and then water-extracted for a second extraction, thereby improving the extraction rate of the polygonatum polysaccharides and saponins; the added mulberry extract is prepared by a specific method, and since anthocyanins in mulberries are heat-sensitive substances, they will undergo oxidative degradation under high temperature for a long time, resulting in the loss of effective ingredients and reducing the extraction rate. The present invention uses an enzyme method and a microwave method for extraction, which can, on the one hand, extract water-soluble substances such as polysaccharides in mulberries, and on the other hand, greatly shorten the heating time. The microwave radiation causes the system to heat up rapidly, so that the heat transfer and mass transfer directions are consistent, thereby improving the solubility and stability of mulberry anthocyanins, and making the obtained mulberry extract have better biological activity.

[0038] (3) The composite plant extract provided by the present invention is added with a honeysuckle extract. First, γ-cyclodextrin is reacted with dihydroquercetin to form an inclusion compound, thereby improving the water solubility of dihydroquercetin. Subsequently, the reduced pressure internal boiling method is combined with an ultrasonic method to extract the active substances such as chlorogenic acid in the honeysuckle. The chlorogenic acid in the honeysuckle can be extracted at a lower temperature and in a shorter time. At the same time, tea theanine and a mixed solution are added during the extraction process. The dihydroquercetin inclusion compound in the mixed solution can not only improve the extraction rate of chlorogenic acid during the extraction process, but also improve the stability of chlorogenic acid together with tea theanine. Theanine can also synergistically improve the anti-fatigue effect of chlorogenic acid in honeysuckle extract; the inventors of the present application unexpectedly discovered that the γ-cyclodextrin is first formed into an inclusion complex and then added into the honeysuckle extraction process, which can effectively improve the extraction rate and stability of chlorogenic acid in honeysuckle extract, so that the obtained honeysuckle extract has a stronger fatigue relieving effect; at the same time, the dihydroquercetin inclusion complex can also promote the absorption of active substances in the composition, improve the bioavailability of the composition, and make the composite plant extract have better effect of relieving physical fatigue. DETAILED DESCRIPTION

[0039] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] In the present invention, the polygonatum odoratum extract and okra extract were purchased from Xi'an Ruierli Bioengineering Co., Ltd., both of which are water extracts with a specification of 10:1.

[0041] Example 1

[0042] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0043] 30 parts of polygonatum sibiricum extract, 20 parts of honeysuckle extract, 13 parts of polygonatum odoratum extract, 8 parts of okra extract, and 7 parts of mulberry extract.

[0044] Wherein, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0045] 45g of polygonatum powder was added to 1L of petroleum ether, refluxed at 55°C for 4h for degreasing, and then dried to obtain defatted polygonatum powder; 35g of defatted polygonatum powder was added to 350g of ethanol aqueous solution (the volume ratio of ethanol to water was 7.5:2.5), and extracted for 30min at an ultrasonic power of 180W and a temperature of 60°C. After the extraction was completed, it was filtered to obtain an extract, and the filter residue was dried for later use; the filter residue was added to deionized water (the material-liquid ratio was 1:13), and then 3% of cellulase by weight of the filter residue and 2% of papain by weight of the filter residue were added, and enzymolysis was carried out at 43°C for 40min. After the enzymolysis was completed, the enzyme was inactivated, and water extraction was carried out at 65°C for 1.5h. After the water extraction was completed, it was filtered to obtain a water extract; the extract and the water extract were combined, and the solvent was removed by reduced pressure rotary evaporation at 15kpa to obtain a polygonatum extract.

[0046] The preparation method of the honeysuckle extract comprises the following steps:

[0047] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0048] The preparation method of the mulberry extract comprises the following steps:

[0049] Add 45g of mulberry powder to 1L of petroleum ether, reflux at 55°C for 4h, and then dry to obtain defatted mulberry powder; add 35g of defatted mulberry powder to 420g of water, soak at 35°C for 2.5h, then add 1g of cellulase and 0.7g of pectinase, and perform enzymolysis at 43°C for 40min. After the enzymolysis is completed, inactivate the enzyme, treat at a microwave power of 350W for 6min, filter after the treatment, and evaporate the filtrate under reduced pressure at 15kpa to obtain mulberry extract.

[0050] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0051] The raw materials are weighed according to the above formula, and the polygonatum sibiricum extract, honeysuckle extract, polygonatum odoratum extract, okra extract and mulberry extract are mixed evenly to obtain the product.

[0052] Example 2

[0053] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0054] 25 parts of polygonatum sibiricum extract, 15 parts of honeysuckle extract, 10 parts of polygonatum odoratum extract, 5 parts of okra extract, and 5 parts of mulberry extract.

[0055] Wherein, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0056] 40g of polygonatum powder was added to 1L of petroleum ether, refluxed at 50°C for 5h for degreasing, and then dried to obtain defatted polygonatum powder; 30g of defatted polygonatum powder was added to 240g of ethanol aqueous solution (the volume ratio of ethanol to water was 8:2), and extracted for 40min at an ultrasonic power of 150W and a temperature of 55°C. After the extraction was completed, the extract was filtered to obtain an extract, and the filter residue was dried for later use; the filter residue was added to deionized water (the material-liquid ratio was 1:10), and then 2% of cellulase by weight of the filter residue and 1% of papain by weight of the filter residue were added, and enzymolysis was carried out at 40°C for 50min. After the enzymolysis was completed, the enzyme was inactivated, and the extract was extracted at 60°C for 2h. After the extraction was completed, the extract was filtered to obtain a water extract; the extract and the water extract were combined, and the solvent was removed by reduced pressure rotary evaporation at 10kPa to obtain a polygonatum extract.

[0057] The preparation method of the honeysuckle extract comprises the following steps:

[0058] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 0.5%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 4%; then add 30g of dihydroquercetin solution to 60g of γ-cyclodextrin solution, stir at a constant temperature of 38°C for 8h to obtain a mixed solution; add 40g of honeysuckle pollen to 100g of ethanol aqueous solution (the mass concentration of ethanol is 80%), soak for 30min at room temperature, add 900g of hot water at 80°C after the soaking, then add 0.5g of tea theanine and 90g of the mixed solution, extract under reduced pressure for 10min at an ultrasonic power of 200W and a pressure of 20kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 20kpa to obtain a honeysuckle extract.

[0059] The preparation method of the mulberry extract comprises the following steps:

[0060] 40 g of mulberry powder was added to 1 L of petroleum ether, refluxed at 50 ° C for 5 h, and then dried to obtain defatted mulberry powder; 30 g of defatted mulberry powder was added to 300 g of water, soaked at 30 ° C for 3 h, then 0.6 g of cellulase and 0.1 g of pectinase were added, and enzymolysis was performed at 40 ° C for 50 min. After the enzymolysis was completed, the enzyme was inactivated, and the mixture was treated at a microwave power of 300 W for 8 min. After the treatment was completed, it was filtered, and the filtrate was evaporated under reduced pressure at 10 kPa to obtain mulberry extract.

[0061] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0062] The raw materials are weighed according to the above formula, and the polygonatum sibiricum extract, honeysuckle extract, polygonatum odoratum extract, okra extract and mulberry extract are mixed evenly to obtain the product.

[0063] Example 3

[0064] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0065] 35 parts of polygonatum sibiricum extract, 25 parts of honeysuckle extract, 15 parts of polygonatum odoratum extract, 10 parts of okra extract, and 10 parts of mulberry extract.

[0066] Wherein, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0067] 50g of polygonatum powder was added to 1L of petroleum ether, refluxed at 60°C for 3h for degreasing, and then dried to obtain defatted polygonatum powder; 40g of defatted polygonatum powder was added to 600g of ethanol aqueous solution (the volume ratio of ethanol to water was 7:3), and extracted for 20min at an ultrasonic power of 200W and a temperature of 65°C. After the extraction was completed, the extract was filtered to obtain an extract, and the filter residue was dried for later use; the filter residue was added to deionized water (the material-liquid ratio was 1:15), and then 4% of cellulase by weight of the filter residue and 3% of papain by weight of the filter residue were added, and enzymolysis was carried out at 45°C for 30min. After the enzymolysis was completed, the enzyme was inactivated, and the solution was extracted at 70°C for 1h. After the extraction was completed, the solution was filtered to obtain a water extract; the extract and the water extract were combined, and the solvent was removed by reduced pressure rotary evaporation at 20kPa to obtain a polygonatum extract.

[0068] The preparation method of the honeysuckle extract comprises the following steps:

[0069] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1.5%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 6%; then add 20g of dihydroquercetin solution to 40g of γ-cyclodextrin solution, stir at a constant temperature of 45°C for 5h to obtain a mixed solution; add 50g of honeysuckle pollen to 140g of ethanol aqueous solution (the mass concentration of ethanol is 70%), soak at room temperature for 40min, add 1200g of hot water with a temperature of 70°C after the soaking, then add 1g of tea theanine and 60g of the mixed solution, extract under reduced pressure at an ultrasonic power of 300W and a pressure of 30kpa for 10min, filter after the extraction, and evaporate the filtrate under reduced pressure at 30kpa to obtain a honeysuckle extract.

[0070] The preparation method of the mulberry extract comprises the following steps:

[0071] Add 50g of mulberry powder to 1L of petroleum ether, reflux at 60℃ for 3h, and then dry to obtain defatted mulberry powder; add 40g of defatted mulberry powder to 600g of water, soak at 40℃ for 2h, then add 1.6g of cellulase and 1.2g of pectinase, and perform enzymolysis at 45℃ for 30min. After the enzymolysis is completed, inactivate the enzyme, treat at a microwave power of 400W for 4min, filter after the treatment, and evaporate the filtrate under reduced pressure at 20kPa to obtain mulberry extract.

[0072] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0073] The raw materials are weighed according to the above formula, and the polygonatum sibiricum extract, honeysuckle extract, polygonatum odoratum extract, okra extract and mulberry extract are mixed evenly to obtain the product.

[0074] Comparative Example 1

[0075] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0076] 30 parts of polygonatum sibiricum extract, 20 parts of honeysuckle extract, 13 parts of polygonatum odoratum extract, 8 parts of okra extract, and 7 parts of mulberry extract.

[0077] Wherein, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0078] 45g of polygonatum powder was added to 1L of petroleum ether, refluxed at 55°C for 4h for degreasing, and then dried to obtain defatted polygonatum powder; 35g of defatted polygonatum powder was added to 350g of ethanol aqueous solution (the volume ratio of ethanol to water was 7.5:2.5), and extracted for 30min at an ultrasonic power of 180W and a temperature of 60°C. After the extraction was completed, it was filtered to obtain an extract, and the filter residue was dried for later use; the filter residue was added to deionized water (the material-liquid ratio was 1:13), and then 3% of cellulase by weight of the filter residue and 2% of papain by weight of the filter residue were added, and enzymolysis was carried out at 43°C for 40min. After the enzymolysis was completed, the enzyme was inactivated, and water extraction was carried out at 65°C for 1.5h. After the water extraction was completed, it was filtered to obtain a water extract; the extract and the water extract were combined, and the solvent was removed by reduced pressure rotary evaporation at 15kpa to obtain a polygonatum extract.

[0079] The preparation method of the honeysuckle extract comprises the following steps:

[0080] Add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35 minutes, add 1100g of hot water at 75°C after soaking, then add 0.8g of tea theanine, 0.25g of dihydroquercetin, and 2.5g of γ-cyclodextrin, and extract under reduced pressure for 15 minutes at an ultrasonic power of 250W and a pressure of 25kpa. After the extraction is completed, filter and evaporate the filtrate under reduced pressure at 25kpa to obtain the honeysuckle extract.

[0081] The preparation method of the mulberry extract comprises the following steps:

[0082] Add 45g of mulberry powder to 1L of petroleum ether, reflux at 55°C for 4h, and then dry to obtain defatted mulberry powder; add 35g of defatted mulberry powder to 420g of water, soak at 35°C for 2.5h, then add 1g of cellulase and 0.7g of pectinase, and perform enzymolysis at 43°C for 40min. After the enzymolysis is completed, inactivate the enzyme, treat at a microwave power of 350W for 6min, filter after the treatment, and evaporate the filtrate under reduced pressure at 15kpa to obtain mulberry extract.

[0083] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0084] The raw materials are weighed according to the above formula, and the polygonatum sibiricum extract, honeysuckle extract, polygonatum odoratum extract, okra extract and mulberry extract are mixed evenly to obtain the product.

[0085] Compared with Example 1, in this comparative example, dihydroquercetin and γ-cyclodextrin are not included to form an inclusion complex, but the two are directly added to the honeysuckle extract.

[0086] Comparative Example 2

[0087] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0088] 20 parts of honeysuckle extract, 13 parts of polygonatum odoratum extract, 8 parts of okra extract, and 7 parts of mulberry extract.

[0089] Wherein, the preparation method of the honeysuckle extract comprises the following steps:

[0090] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0091] The preparation method of the mulberry extract comprises the following steps:

[0092] Add 45g of mulberry powder to 1L of petroleum ether, reflux at 55°C for 4h, and then dry to obtain defatted mulberry powder; add 35g of defatted mulberry powder to 420g of water, soak at 35°C for 2.5h, then add 1g of cellulase and 0.7g of pectinase, and perform enzymolysis at 43°C for 40min. After the enzymolysis is completed, inactivate the enzyme, treat at a microwave power of 350W for 6min, filter after the treatment, and evaporate the filtrate under reduced pressure at 15kpa to obtain mulberry extract.

[0093] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0094] Weigh the raw materials according to the above formula, and evenly mix the honeysuckle extract, polygonatum odoratum extract, okra extract and mulberry extract to obtain the product.

[0095] Compared with Example 1, the present comparative example did not add the polygonatum sibiricum extract.

[0096] Comparative Example 3

[0097] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0098] 30 parts of polygonatum sibiricum extract, 20 parts of honeysuckle extract, 8 parts of okra extract, and 7 parts of mulberry extract.

[0099] Wherein, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0100] 45g of polygonatum powder was added to 1L of petroleum ether, refluxed at 55°C for 4h for degreasing, and then dried to obtain defatted polygonatum powder; 35g of defatted polygonatum powder was added to 350g of ethanol aqueous solution (the volume ratio of ethanol to water was 7.5:2.5), and extracted for 30min at an ultrasonic power of 180W and a temperature of 60°C. After the extraction was completed, it was filtered to obtain an extract, and the filter residue was dried for later use; the filter residue was added to deionized water (the material-liquid ratio was 1:13), and then 3% of cellulase by weight of the filter residue and 2% of papain by weight of the filter residue were added, and enzymolysis was carried out at 43°C for 40min. After the enzymolysis was completed, the enzyme was inactivated, and water extraction was carried out at 65°C for 1.5h. After the water extraction was completed, it was filtered to obtain a water extract; the extract and the water extract were combined, and the solvent was removed by reduced pressure rotary evaporation at 15kpa to obtain a polygonatum extract.

[0101] The preparation method of the honeysuckle extract comprises the following steps:

[0102] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0103] The preparation method of the mulberry extract comprises the following steps:

[0104] Add 45g of mulberry powder to 1L of petroleum ether, reflux at 55°C for 4h, and then dry to obtain defatted mulberry powder; add 35g of defatted mulberry powder to 420g of water, soak at 35°C for 2.5h, then add 1g of cellulase and 0.7g of pectinase, and perform enzymolysis at 43°C for 40min. After the enzymolysis is completed, inactivate the enzyme, treat at a microwave power of 350W for 6min, filter after the treatment, and evaporate the filtrate under reduced pressure at 15kpa to obtain mulberry extract.

[0105] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0106] Weigh the raw materials according to the above formula, and evenly mix the polygonatum sibiricum extract, honeysuckle extract, okra extract and mulberry extract to obtain the product.

[0107] Compared with Example 1, the present comparative example does not add the Polygonatum odoratum extract.

[0108] Comparative Example 4

[0109] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0110] 30 parts of polygonatum sibiricum extract, 20 parts of honeysuckle extract, 13 parts of polygonatum odoratum extract, and 7 parts of mulberry extract.

[0111] Wherein, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0112] 45g of polygonatum powder was added to 1L of petroleum ether, refluxed at 55°C for 4h for degreasing, and then dried to obtain defatted polygonatum powder; 35g of defatted polygonatum powder was added to 350g of ethanol aqueous solution (the volume ratio of ethanol to water was 7.5:2.5), and extracted for 30min at an ultrasonic power of 180W and a temperature of 60°C. After the extraction was completed, it was filtered to obtain an extract, and the filter residue was dried for later use; the filter residue was added to deionized water (the material-liquid ratio was 1:13), and then 3% of cellulase by weight of the filter residue and 2% of papain by weight of the filter residue were added, and enzymolysis was carried out at 43°C for 40min. After the enzymolysis was completed, the enzyme was inactivated, and water extraction was carried out at 65°C for 1.5h. After the water extraction was completed, it was filtered to obtain a water extract; the extract and the water extract were combined, and the solvent was removed by reduced pressure rotary evaporation at 15kpa to obtain a polygonatum extract.

[0113] The preparation method of the honeysuckle extract comprises the following steps:

[0114] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0115] The preparation method of the mulberry extract comprises the following steps:

[0116] Add 45g of mulberry powder to 1L of petroleum ether, reflux at 55°C for 4h, and then dry to obtain defatted mulberry powder; add 35g of defatted mulberry powder to 420g of water, soak at 35°C for 2.5h, then add 1g of cellulase and 0.7g of pectinase, and perform enzymolysis at 43°C for 40min. After the enzymolysis is completed, inactivate the enzyme, treat at a microwave power of 350W for 6min, filter after the treatment, and evaporate the filtrate under reduced pressure at 15kpa to obtain mulberry extract.

[0117] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0118] Weigh the raw materials according to the above formula, and evenly mix the polygonatum sibiricum extract, honeysuckle extract, polygonatum odoratum extract and mulberry extract to obtain the product.

[0119] Compared with Example 1, no okra extract was added in this comparative example.

[0120] Comparative Example 5

[0121] A composite plant extract having the function of relieving physical fatigue, comprising the following raw materials by weight:

[0122] 30 parts of polygonatum sibiricum extract, 20 parts of honeysuckle extract, 13 parts of polygonatum odoratum extract, and 8 parts of okra extract.

[0123] Wherein, the preparation method of the polygonatum sibiricum extract comprises the following steps:

[0124] 45g of polygonatum powder was added to 1L of petroleum ether, refluxed at 55°C for 4h for degreasing, and then dried to obtain defatted polygonatum powder; 35g of defatted polygonatum powder was added to 350g of ethanol aqueous solution (the volume ratio of ethanol to water was 7.5:2.5), and extracted for 30min at an ultrasonic power of 180W and a temperature of 60°C. After the extraction was completed, it was filtered to obtain an extract, and the filter residue was dried for later use; the filter residue was added to deionized water (the material-liquid ratio was 1:13), and then 3% of cellulase by weight of the filter residue and 2% of papain by weight of the filter residue were added, and enzymolysis was carried out at 43°C for 40min. After the enzymolysis was completed, the enzyme was inactivated, and water extraction was carried out at 65°C for 1.5h. After the water extraction was completed, it was filtered to obtain a water extract; the extract and the water extract were combined, and the solvent was removed by reduced pressure rotary evaporation at 15kpa to obtain a polygonatum extract.

[0125] The preparation method of the honeysuckle extract comprises the following steps:

[0126] Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution with a mass concentration of 1%; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution with a mass concentration of 5%; then add 25g of dihydroquercetin solution to 50g of γ-cyclodextrin solution, stir at a constant temperature of 42°C for 7h to obtain a mixed solution; add 45g of honeysuckle pollen to 120g of ethanol aqueous solution (the mass concentration of ethanol is 75%), soak at room temperature for 35min, add 1100g of hot water with a temperature of 75°C after the soaking, then add 0.8g of tea theanine and 75g of the mixed solution, extract under reduced pressure for 15min at an ultrasonic power of 250W and a pressure of 25kpa, filter after the extraction, and evaporate the filtrate under reduced pressure at 25kpa to obtain a honeysuckle extract.

[0127] A method for preparing a composite plant extract having the function of relieving physical fatigue comprises the following steps:

[0128] The raw materials are weighed according to the above formula, and the polygonatum sibiricum extract, honeysuckle extract, polygonatum odoratum extract and okra extract are evenly mixed to obtain the product.

[0129] Compared with Example 1, no mulberry extract was added in this comparative example.

[0130] The extraction rates of the honeysuckle extracts in Examples 1-3 and Comparative Example 1 were calculated and the chlorogenic acid content was determined. The results are shown in Table 1 below:

[0131] Extraction rate = (weight of honeysuckle extract - weight of dihydroquercetin - weight of γ-cyclodextrin - weight of theanine) / weight of honeysuckle powder × 100%

[0132] Table 1 Extraction rate and chlorogenic acid content of honeysuckle extract

[0133] Group Dihydroquercetin, γ-cyclodextrin and theanine weight / g Extraction rate / % Chlorogenic acid content / % Example 1 0.25g / 2.5g / 0.8g 15.8 31.7 Example 2 0.15g / 2.4g / 0.5g 14.6 29.4 Example 3 0.3g / 2.4g / 1g 15.5 30.9 Comparative Example 1 0.25g / 2.5g / 0.8g 13.3 24.6

[0134] It can be seen from Table 1 above that the preparation method of the honeysuckle extract provided by the present invention can significantly increase the content of chlorogenic acid in the extract, make the chlorogenic acid in the honeysuckle extract have better stability, and improve the quality of the honeysuckle extract.

[0135] The composite plant extracts prepared in Examples 1-3 and Comparative Examples 1-5 were subjected to mouse tests to verify their efficacy in relieving physical fatigue, as follows:

[0136] Experimental animal grouping: 270 SPF grade Kunming male mice were randomly divided into a blank group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, 30 mice in each group. SPF grade Kunming male mice, 6 to 8 weeks old, weighing 20 ± 2.0g. The experimental animals were raised in a barrier environment animal room with a temperature of 22 ° C, a humidity of 40-70%, 12h light and dark alternation, fed with standard feed, free drinking water and food, and adaptive feeding for 1 week. After the adaptive feeding was completed, the composite plant extracts in Examples 1-3 were gavaged respectively, and the composite plant extracts in Comparative Examples 1-5 were gavaged respectively, with a gavage dose of 300 mg / kg / day, and the blank group was gavaged with an equal amount of physiological saline for 30 consecutive days.

[0137] 1. Weighted swimming test: On the 30th day of the test, 30 minutes after the last oral administration of the mice, 8 mice were randomly selected from each group, and a lead block of 5% of their body weight was placed at the base of the mouse's tail. The mice were placed in a swimming box with a water depth of 30 cm and a water temperature of 25±1.0℃, and the time from the beginning of the mouse's swimming to its death was recorded. The test results are shown in Table 2 below:

[0138] 2. Serum urea determination: On the 30th day of the experiment, 30 minutes after the last oral administration of the mice, 8 mice were randomly selected from each group and swam for 90 minutes without weight in water at a temperature of 30°C. Blood was collected after resting for 60 minutes. The eyeballs were removed and about 0.5 mL of whole blood (without anticoagulant) was collected. The blood was placed in a 4°C refrigerator for about 3 hours. After the blood coagulated, it was centrifuged at 2000r / min for 15 minutes, and the serum was collected. The mouse serum urea was determined using a kit. The test results are shown in Table 2:

[0139] 3. Determination of liver glycogen: On the 30th day of the experiment, 30 minutes after the last oral administration of the mice, 7 mice were randomly selected from each group, and the mice were killed. The liver was rinsed with physiological saline and dried with filter paper. 100 mg of liver was accurately weighed, and 8 mL of TCA was added. Each tube was homogenized for 1 minute, and the homogenate was poured into a centrifuge tube, centrifuged at 3000 r / min for 15 minutes, and the supernatant was transferred to another test tube. The supernatant was taken to determine the liver glycogen content by the anthrone method. The test results are shown in Table 3 below:

[0140] 4. Blood lactate determination: On the 30th day of the experiment, 7 mice were randomly selected from each group, and blood was collected 30 minutes after the last gavage. Blood was collected from the inner canthus with a capillary tube, and then they swam in water at 30°C without weight for 10 minutes before stopping. Lactate meter determination method: Before swimming, 20μL of blood was collected and added to 40μL of membrane permeabilization solution, and the cells were immediately and fully shaken; immediately after swimming, 20μL of blood was collected and added to 40μL of membrane permeabilization solution and shaken; after resting for 20 minutes, 20μL of blood was collected and added to 40μL of membrane permeabilization solution and shaken, and measured with a lactate meter. The area under the blood lactate curve was calculated according to the following formula, and the test results are shown in Table 3:

[0141] The area under the blood lactate curve = 5 × (blood lactate value before swimming + 3 × blood lactate value 0 min after swimming + 2 × blood lactate value 20 min after resting after swimming)

[0142] Table 2 Effects of compound plant extracts on weight-bearing swimming time and serum urea in mice

[0143] Group Number of animals Weighted swimming time (s) Serum urea nitrogen (mmol / L) Blank Group 8 329±93 8.25±0.87 Example 1 Group 8 495±126** 6.71±0.58** Example 2 Group 8 478±102** 6.97±0.72** Example 3 Group 8 490±114** 6.80±0.64** Comparative Example 1 8 461±107* 7.08±0.69** Comparative Example 2 8 432±95* 7.29±0.81* Comparative Example 3 8 438±99* 7.36±0.78* Comparative Example 4 8 445±106* 7.14±0.73* Comparative Example 5 8 457±115* 7.22±0.67*

[0144] Note: * indicates P < 0.05 compared with the blank control group, ** indicates P < 0.01 compared with the blank control group

[0145] As can be seen from Table 2 above, compared with the blank group, the weighted swimming time of mice in the Example 1-3 group and the Comparative Example 1-5 group was prolonged, with significant differences (P < 0.05), indicating that the composite plant extract prepared by the present invention has the effect of prolonging the weighted swimming time of mice; and the Example 1-3 group is better than the Comparative Example 1-5 group, with extremely significant differences compared with the blank group (P < 0.01), indicating that the raw materials of the composite plant extract of the present invention have a synergistic effect. Compared with the blank group, the serum urea nitrogen of mice in the Example 1-3 group and the Comparative Example 1-5 group was significantly decreased, with significant differences (P < 0.05), indicating that the composite plant extract prepared by the present invention can inhibit the accumulation of serum urea nitrogen in mice after exercise, and the effect of the Example 1-3 group is better than the Comparative Example 1-5 group, indicating that the raw materials of the composite plant extract of the present invention have a synergistic effect.

[0146] Table 3 Effects of compound plant extracts on liver glycogen and blood lactate in mice

[0147] Group Number of animals Glycogen (mg / g liver) Area under the blood lactate curve (mmol / L) Blank Group 7 8.31±1.04 125.3±18.6 Example 1 Group 7 11.28±1.45** 96.5±13.2** Example 2 Group 7 10.85±1.32** 101.6±14.5* Example 3 Group 7 11.02±1.26** 99.4±14.8* Comparative Example 1 7 10.26±1.37* 101.8±15.2* Comparative Example 2 7 9.67±1.09* 107.6±16.7 Comparative Example 3 7 9.83±1.12* 104.1±14.3* Comparative Example 4 7 10.05±1.28* 102.9±13.6* Comparative Example 5 7 10.19±1.41* 103.7±15.9*

[0148] Note: * indicates P < 0.05 compared with the blank control group, ** indicates P < 0.01 compared with the blank control group

[0149] As can be seen from Table 3, compared with the blank group, the liver glycogen of mice in the Example 1-3 group and the Comparative Example 1-5 group increased, with significant differences (P < 0.05), indicating that the composite plant extract prepared by the present invention has the effect of improving the glycogen reserve level; and the Example 1-3 group is better than the Comparative Example 1-5 group, with a very significant difference compared with the blank group (P < 0.01), indicating that the raw materials of the composite plant extract of the present invention have a synergistic effect. Compared with the blank group, the area under the lactic acid curve of mice in the Example 1-3 group and the Comparative Example 1-5 group decreased significantly, with significant differences (P < 0.05), indicating that the composite plant extract prepared by the present invention can relieve physical fatigue.

[0150] According to the results of the physical fatigue relief test method in the "Technical Specifications for Inspection and Evaluation of Health Foods", the composite plant extract prepared by the present invention was positive in the weighted swimming test, and was positive in the three biochemical indicators of blood lactate, serum urea, and liver glycogen / muscle glycogen. It can be determined that the composite plant extract prepared by the present invention has the effect of relieving physical fatigue.

[0151] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A composite plant extract having the function of relieving physical fatigue, characterized in that: By weight, it includes the following raw materials: 25-35 parts of honeysuckle extract, 15-25 parts of polygonatum extract, 10-15 parts of polygonatum odoratum extract, 5-10 parts of okra extract, and 5-10 parts of mulberry extract.

2. The composite plant extract according to claim 1, characterized in that By weight, it includes the following raw materials: 30-35 parts of honeysuckle extract, 15-20 parts of polygonatum sibiricum extract, 10-13 parts of polygonatum odoratum extract, 5-8 parts of okra extract, and 5-7 parts of mulberry extract.

3. The composite plant extract according to claim 1, characterized in that The preparation method of the honeysuckle extract comprises the following steps: Add dihydroquercetin to anhydrous ethanol, stir evenly to obtain a dihydroquercetin solution; add γ-cyclodextrin to deionized water, stir evenly to obtain a γ-cyclodextrin solution; then add the dihydroquercetin solution to the γ-cyclodextrin solution, stir at a constant temperature to obtain a mixed solution; add honeysuckle pollen to the ethanol-water solution, soak at room temperature, add 70-80°C hot water after the soaking, then add tea theanine and the mixed solution, extract under reduced pressure under ultrasonic conditions, filter after the extraction, and perform reduced pressure rotary evaporation on the filtrate to obtain a honeysuckle extract.

4. The composite plant extract according to claim 3, characterized in that The mass concentration of the dihydroquercetin solution is 0.5-1.5%, the mass concentration of the γ-cyclodextrin solution is 4-6%, the mass ratio of the dihydroquercetin solution to the γ-cyclodextrin solution is 20-30:40-60, the temperature of the constant temperature stirring is 38-45°C, and the time is 5-8h; the mass concentration of ethanol in the ethanol-water solution is 70-80%, the mass ratio of the honeysuckle pollen, the ethanol-water solution, hot water, tea theanine, and the mixed solution is 40-50:100-140:900-1200:0.5-1:60-90, the immersion time is 30-40min, the power of the ultrasound is 200-300W, the pressure of the reduced pressure extraction is 20-30kpa, and the time is 10-20min.

5. The composite plant extract according to claim 1, characterized in that The preparation method of the polygonatum sibiricum extract comprises the following steps: The polygonatum powder is added to petroleum ether, refluxed at 50-60°C for 3-5h for degreasing, and then dried to obtain defatted polygonatum powder; the defatted polygonatum powder is added to ethanol aqueous solution for ultrasonic extraction, filtered after the extraction is completed to obtain an extract, and the filter residue is dried for later use; the filter residue is added to deionized water, followed by cellulase and papain for enzymolysis, after the enzymolysis is completed, the enzyme is inactivated, and then water extraction is performed, after the water extraction is completed, it is filtered to obtain a water extract; the extract and the water extract are combined, and reduced pressure rotary evaporation is performed to obtain the polygonatum extract.

6. The composite plant extract according to claim 5, characterized in that The volume ratio of ethanol to water in the ethanol aqueous solution is 7-8:2-3, the solid-liquid ratio of the defatted polygonatum and the ethanol aqueous solution is 1:8-12, the power of the ultrasonic extraction is 150-200W, the temperature is 55-65°C, and the time is 20-40min; the solid-liquid ratio of the filter residue and deionized water is 1:10-15, the added amount of the cellulase is 2-4% of the mass of the filter residue, the added amount of the papain is 1-3% of the mass of the filter residue, the temperature of the enzymatic hydrolysis is 40-45°C, and the time is 30-50min; the temperature of the water extraction is 60-70°C, and the time is 1-2h; the pressure of the vacuum rotary evaporation is 10-20kpa.

7. The composite plant extract according to claim 1, characterized in that The preparation method of the mulberry extract comprises the following steps: The mulberry powder is added to petroleum ether, refluxed at 50-60°C for 3-5h, and then dried to obtain defatted mulberry powder; the defatted mulberry powder is added to water, and cellulase and pectinase are added after immersion to perform enzymolysis, and the enzyme is inactivated after the enzymolysis is completed, followed by microwave treatment, and after the treatment is completed, the powder is filtered, and the filtrate is subjected to reduced pressure rotary evaporation to obtain a mulberry extract.

8. The composite plant extract according to claim 7, characterized in that The solid-liquid ratio of the defatted mulberry powder to water is 1:10-15; the immersion temperature is 30-40°C, and the time is 2-3h; the amount of the cellulase added is 2-4% of the mass of the defatted mulberry powder, the amount of the pectinase added is 1-3% of the mass of the defatted mulberry powder, the temperature of the enzymolysis is 40-45°C, and the time is 30-50min; the power of the microwave treatment is 300-400W, and the time is 4-8min; the pressure of the reduced pressure rotary evaporation is 10-20kpa.

9. A method for preparing the composite plant extract having the function of relieving physical fatigue as claimed in any one of claims 1 to 8, characterized in that: The following steps are involved: The raw materials are weighed according to the formula, and the honeysuckle extract, polygonatum sibiricum extract, polygonatum odoratum extract, okra extract and mulberry extract are evenly mixed to obtain the composite plant extract.

10. Use of the composite plant extract having the function of relieving physical fatigue as claimed in any one of claims 1 to 8 in food or medicine.